SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_02250 FitnessBrowser__Caulo:CCNA_02250 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WPQ3 Biotin-dependent 3-methylcrotonyl-coenzyme A carboxylase alpha1 subunit; EC 6.3.4.14 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
47% identity, 99% coverage: 1:650/654 of query aligns to 1:653/654 of P9WPQ3

query
sites
P9WPQ3

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K322 (≠ P325) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

3u9sA Crystal structure of p. Aeruginosa 3-methylcrotonyl-coa carboxylase (mcc) 750 kd holoenzyme, coa complex (see paper)
46% identity, 99% coverage: 2:650/654 of query aligns to 4:621/621 of 3u9sA

query
sites
3u9sA

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V

A

K

E

A

L

L

S

Y

A

C

V

S

A

E

G

G

G

E

Q

L

V

V

S

E

E

E

G

G

V

T

V

P

L

L

A

V

R

E

L

L

E

D

active site: E263 (= E275), E276 (= E293), N278 (= N295), E284 (= E301), R326 (= R343), L502 (= L515)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: M586 (= M615), K587 (= K616)

Q5LUF3 Propionyl-CoA carboxylase alpha chain; EC 6.4.1.3 from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) (see paper)
49% identity, 68% coverage: 1:445/654 of query aligns to 1:461/681 of Q5LUF3

query
sites
Q5LUF3

M

M

L

F

S

N

S

K

V

I

L

L

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

R

C

R

R

I

V

I

I

R

K

T

T

A

A

R

R

E

K

L

M

G

G

V

I

R

S

T

T

I

V

A

A

V

I

Y

Y

S

S

E

D

A

A

D

D

A

K

N

Q

A

A

P

L

F

H

V

V

M

Q

E

M

A

A

D

D

A

E

A

A

I

V

L

H

I

I

G

G

P

P

A

P

P

P

A

A

K

N

E

Q

S

S

Y

Y

L

I

D

V

P

I

R

D

K

K

I

V

L

M

A

A

A

A

A

I

R

R

Q

A

M

T

G

G

A

A

E

Q

A

A

I

V

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

S

E

K

F

F

A

A

Q

E

S

A

V

L

I

E

D

A

A

E

G

G

L

V

V

I

W

F

I

V

G

G

P

P

P

P

P

K

S

G

A

A

I

I

R

E

A

A

M

M

G

G

L

D

K

K

D

I

A

T

A

S

K

K

A

K

V

I

M

A

I

Q

K

E

A

A

G

N

V

V

P

S

T

T

T

V

P

P

G

G

Y

Y

L

M

G

G

E

L

D

I

Q

E

S

D

V

A

E

D

R

E

L

A

T

V

V

K

E

I

A

S

A

N

K

Q

I

I

G

G

Y

Y

P

P

V

V

L

M

I

I

K

K

A

A

V

S

A

A

G

G

G

G

G

G

G

G

K

K

G

G

M

M

R

R

K

I

V

A

E

W

R

N

A

D

E

Q

D

E

F

A

E

R

A

E

A

G

L

F

G

Q

S

S

C

S

R

K

R

N

E

E

A

A

S

A

A

N

A

S

F

F

G

G

D

D

D

D

R

R

V

I

L

F

L

I

E

E

K

K

Y

F

V

V

T

T

R

Q

P

P

R

R

H

H

I

I

E

E

V

I

Q

Q

V

V

F

L

G

C

D

D

S

S

H

H

G

G

N

N

V

G

V

I

H

Y

L

L

F

G

E

E

R

R

D

E

C

C

S

S

L

I

Q

Q

R

R

R

R

H

N

Q

Q

K

K

V

V

I

V

E

E

E

E

A

A

P

P

A

S

P

P

G

F

M

L

D

D

E

E

A

A

T

T

R

R

E

R

A

A

V

M

C

G

A

E

A

Q

A

A

V

V

K

A

A

L

A

A

Q

K

A

A

V

V

N

G

Y

Y

V

A

G

S

A

A

G

G

T

T

V

V

E

E

F

F

I

I

A

V

D

D

A

G

S

Q

E

K

G

N

L

F

R

-

A

-

D

-

R

-

I

-

W

Y

F

F

M

L

E

E

M

M

N

N

T

T

R

R

L

L

Q

Q

V

V

E

E

H

H

P

P

V

V

T

T

E

E

M

L

V

I

T

T

G

G

Q

V

D

D

L

L

V

V

E

E

W

Q

Q

M

L

I

R

R

V

V

A

A

S

A

G

G

E

E

P

P

L

L

P

S

L

I

E

T

Q

Q

D

G

E

D

I

V

T

K

L

L

D

T

G

G

W

W

A

A

M

I

E

E

A

N

R

R

L

L

Y

Y

A

A

E

E

N

D

P

P

A

Y

T

R

G

G

F
|
F

L

L

P

P

S

S

T

I

G

G

K

R

L

L

K

T

H

R

F

Y

R

R

L

P

P

P

-

A

-

E

-

T

-

A

-

A

-

G

-

P

-

L

-

L

-

V

-

N

-

G

-

K

-

W

E

Q

G

G

D

D

-

A

-

P

-

S

-

G

-

E

-

A

-

A

V

V

R

R

V

N

D

D

S

T

A

G

V

V

E

Y

E

E

G

G

G

G

E

E

V

I

T

S

P

M

F

Y

Y

Y

D

D

P

P

M

M

I

I

A

A

K

K

L

L

I

C

A

T

H

W

G

A

A

P

D

T

R

R

E

A

D

A

A

A

A

I

Q

E

R

A

L

M

A

R

E

I

A

A

C

L

A

D

L

S

V

F

E

E

V

V

W

E

P

G

V

I

K

G

T

H

N

N

A

L

A

P

F

F

L

L

A

S

K

A

C

V

A

M

S

D

H

H

P

P

D

K

F

F

V

I

D

S

G

G

A

D

V

M

D

T

T

T

G

A

F

F

I

I

F348 (= F353) binding

Sites not aligning to the query:

515 W→L: No effect on holoenzyme formation.
599 L→A: Loss of holoenzyme formation; when associated with A-602 and A-603.
602 L→A: Loss of holoenzyme formation; when associated with A-602 and A-603.
603 M→A: No effect on holoenzyme formation. Loss of holoenzyme formation; when associated with A-602 and A-603.
647 modified: N6-biotinyllysine

7ybuA Propionyl-CoA carboxylase beta chain, mitochondrial
40% identity, 99% coverage: 2:650/654 of query aligns to 5:670/670 of 7ybuA

query
sites
7ybuA

L

F

S

D

S

K

V

I

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

R

C

R

R

I

V

I

I

R

R

T

T

A

C

R

K

E

K

L

M

G

G

V

I

R

K

T

T

I

V

A

A

V

I

Y

H

S

S

E

D

A

V

D

D

A

A

N

S

A

S

P

V

F

H

V

V

M

K

E

M

A

A

D

D

A

E

A

A

I

V

L

C

I

V

G

G

P

P

A

A

P

P

A

T

K

S

E

K

S

S

Y

Y

L

L

D

N

P

M

R

D

K

A

I

I

L

M

A

E

A

A

A

I

R

K

Q

K

M

T

G

R

A

A

E

Q

A

A

I

V

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

K

E

E

F

F

A

A

Q

R

S

C

V

L

I

A

D

A

A

E

G

D

L

V

V

V

W

F

I

I

G

G

P

P

P

D

P

T

S

H

A

A

I

I

R

Q

A

A

M

M

G

G

L

D

K

K

D

I

A

E

A

S

K

K

A

L

V

L

M

A

I

K

K

K

A

A

G

E

V

V

P

N

T

T

T

I

P

P

G

G

Y

F

L

D

G

G

E

V

D

V

Q

K

S

D

V

A

E

E

R

E

L

A

T

V

V

R

E

I

A

A

A

R

K

E

I

I

G

G

Y

Y

P

P

V

V

L

M

I

I

K

K

A

A

V

S

A

A

G

G

G

G

G

G

G

G

K

K

G

G

M

M

R

R

K

I

V

A

E

W

R

D

A

D

E

E

D

E

F

T

E

R

A

D

A

G

L

F

G

R

S

L

C

S

R

S

R

Q

E

E

A

A

S

A

A

S

A

S

F

F

G

G

D

D

D

D

R

R

V

L

L

L

L

I

E

E

K

K

Y

F

V

I

T

D

R

N

P

P

R

R

H

H

I

I

E

E

V

I

Q

Q

V

V

F

L

G

G

D

D

S

K

H

H

G

G

N

N

V

A

V

L

H

W

L

L

F

N

E

E

R

R

D

E

C

C

S

S

L

I

Q

Q

R

R

R

R

H

N

Q

Q

K

K

V

V

I

V

E

E

E

E

A

A

P

P

A

S

P

I

G

F

M

L

D

D

E

A

A

E

T

T

R

R

E

R

A

A

V

M

C

G

A

E

A

Q

A

A

V

V

K

A

A

L

A

A

Q

R

A

A

V

V

N

K

Y

Y

V

S

G

S

A

A

G

G

T

T

V

V

E

E

F

F

I

L

A

V

D

D

A

S

S

K

E

K

G

N

L

F

R

-

A

-

D

-

R

-

I

-

W

Y

F

F

M

L

E

E

M

M

N

N

T

T

R

R

L

L

Q

Q

V

V

E

E

H

H

P

P

V

V

T

T

E

E

M

C

V

I

T

T

G

G

Q

L

D

D

L

L

V

V

E

Q

W

E

Q

M

L

I

R

R

V

V

A

A

S

K

G

G

E

Y

P

P

L

L

P

R

L

H

E

K

Q

Q

D

A

E

D

I

I

T

R

L

I

D

N

G

G

W

W

A

A

M

V

E

E

A

C

R

R

L

V

Y

Y

A

A

E

E

N

D

P

P

A

Y

T

K

G

S

F

F

-

G

L

L

P

P

S

S

T

I

G

G

K

R

L

L

K

S

H

Q

F

Y

R

Q

L

E

P

P

E

L

G

H

-

L

-

P

D

G

V

V

R

R

V

V

D

D

S

S

A

G

V

I

E

Q

E

P

G

G

G

S

E

D

V

I

T

S

P

I

F

Y

Y

Y

D

D

P

P

M

M

I

I

A

S

K

K

L

L

I

I

A

T

H

Y

G

G

A

S

D

D

R

R

E

T

D

E

A

A

A

L

Q

K

R

R

L

M

A

A

E

D

A

A

C

L

A

D

L

N

V

Y

E

V

V

I

W

R

P

G

V

V

K

T

T

H

N

N

A

I

A

A

F

L

L

L

A

R

K

E

C

V

A

I

S

I

H

N

P

S

D

R

F

F

V

V

D

K

G

G

A

D

V

I

D

S

T

T

G

K

F

F

I

L

E

S

A

D

R

V

L

Y

D

P

E

D

-

G

-

F

-

K

-

G

-

H

-

M

L

L

T

T

E

K

R

S

A

E

F

K

S

N

D

Q

E

L

P

L

A

A

M

I

A

A

A

S

-

S

-

L

-

F

I

V

G

A

W

F

R

Q

L

L

D

R

S

A

F

Q

L

H

E

F

A

Q

E

E

A

N

R

S

R

R

D

M

P

P

-

V

-

I

-

K

-

P

-

D

-

I

-

A

-

N

W

W

E

E

G

-

A

-

P

L

S

S

K

V

L

K

L

L

G

H

F

D

R

K

M

V

N

H

A

T

P

V

R

V

A

A

S

S

M

N

H

N

-

G

-

S

-

V

L

F

P

S

M

V

S

E

T

V

D

D

G

G

K

S

-

K

-

L

-

N

-

V

-

T

-

S

-

T

-

W

-

N

-

L

A

A

T

S

P

P

L

L

R

L

V

S

A

V

L

S

I

V

G

D

G

G

G

T

T

Q

E

R

D

T

W

V

S

Q

W

C

D

L

I

S

R

R

H

E

A

A

D

G

G

G

S

N

T

M

F

-

D

-

E

S

V

I

T

Q

R

F

L

L

P

G

T

T

T

V

Y

Y

G

-

K

K

G

V

P

N

I

I

Q

L

V

T

F

R

E

L

G

A

G

A

D

E

V

L

Q

N

E

K

F

F

D

-

F

-

V

-

A

-

K

M

I

L

G

E

G

K

A

V

G

T

E

E

G

D

G

T

A

S

S

S

D

-

G

-

A

V

I

L

L

R

S

S

P

P

M

M

P

P

G

G

K

V

I

V

V

V

S

A

V

V

S

S

V

V

S

K

A

P

G

G

Q

D

T

A

V

V

S

A

K

E

G

G

Q

Q

T

E

L

I

L

C

T

V

L

I

E

E

A

A

M
|
M

K
|
K

M

M

E

Q

H

N

A

S

M

M

A

T

A

A

P

G

F

K

D

T

G

G

V

T

V

V

A

K

E

S

L

V

S

H

A

C

V

Q

A

A

G

G

G

D

Q

T

V

V

S

G

E

E

G

G

V

D

V

L

L

L

A

V

R

E

L

L

E

E

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: M635 (= M615), K636 (= K616)

P05165 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Homo sapiens (Human) (see 6 papers)
40% identity, 99% coverage: 2:650/654 of query aligns to 63:728/728 of P05165

query
sites
P05165

L

F

S

D

S

K

V

I

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A
|
A

R

C

R
|
R

I

V

I

I

R

R

T

T

A

C

R

K

E

K

L

M

G

G

V

I

R

K

T

T

I

V

A

A

V

I

Y

H

S

S

E

D

A

V

D

D

A

A

N

S

A

S

P

V

F

H

V

V

M

K

E

M

A

A

D

D

A

E

A

A

I

V

L

C

I

V

G

G

P

P

A

A

P

P

A

T

K

S

E

K

S

S

Y

Y

L

L

D

N

P

M

R

D

K

A

I

I

L

M

A

E

A

A

A

I

R

K

Q

K

M

T

G

R

A

A

E

Q

A
|
A

I

V

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

K

E

E

F

F

A

A

Q

R

S

C

V

L

I

A

D

A

A

E

G

D

L

V

V

V

W

F

I
|
I

G

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A75 (= A14) to P: in PA-1; dbSNP:rs794727479
R77 (= R16) to W: in PA-1; loss of function; dbSNP:rs141371306
A138 (= A77) to T: in PA-1; loss of function; dbSNP:rs202247814
I164 (= I103) to T: in PA-1; loss of function; dbSNP:rs202247815
G197 (= G136) to E: in PA-1
M229 (= M168) to K: in PA-1; dbSNP:rs375628794
Q297 (= Q236) to R: in PA-1
D368 (= D312) to G: in PA-1
M373 (≠ Q317) to K: in PA-1; unstable protein; loss of function; dbSNP:rs121964958
G379 (= G323) to V: in PA-1; dbSNP:rs794727087
C398 (≠ A342) to R: in PA-1
R399 (= R343) to Q: in PA-1; dbSNP:rs1301904623
P423 (= P366) to L: in PA-1; dbSNP:rs1443858896
L532 (vs. gap) natural variant: Missing (in PA-1)
V551 (vs. gap) to F: in dbSNP:rs61749895
W559 (= W483) to L: in PA-1; dbSNP:rs118169528
G631 (≠ P546) to R: in PA-1; loss of function; dbSNP:rs796052018
G668 (= G590) to R: in PA-1; loss of biotinylation; dbSNP:rs771438170
K694 (= K616) modified: N6-biotinyllysine; by HLCS
C712 (≠ A634) natural variant: Missing (in PA-1; loss of biotinylation)

Sites not aligning to the query:

1:52 modified: transit peptide, Mitochondrion

3n6rG Crystal structure of the holoenzyme of propionyl-coa carboxylase (pcc) (see paper)
50% identity, 68% coverage: 2:445/654 of query aligns to 1:426/646 of 3n6rG

query
sites
3n6rG

L

F

S

N

S

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A

A

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G

A

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A

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A

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I

A

A

C

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A

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F

E

E

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E

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G

V

I

K

G

T

H

N

N

A

L

A

P

F

F

L

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A

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K

A

C

V

A

M

S

D

H

H

P

P

D

K

F

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active site: K115 (= K116), K157 (= K158), D180 (= D195), H193 (= H208), R219 (= R234), T258 (= T273), E260 (= E275), E273 (= E293), N275 (= N295), R277 (= R297), E281 (= E301), R323 (= R343)

Sites not aligning to the query:

active site: 519
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: 611, 612

8sgxX Leishmania tarentolae propionyl-coa carboxylase (alpha-4-beta-6) (see paper)
38% identity, 99% coverage: 5:650/654 of query aligns to 1:657/657 of 8sgxX

query
sites
8sgxX

V

V

L

L

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N

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V

E

E

K

R

Y

F

V

I

T

D

R

H

P

P

R

R

H

H

I

I

E

E

V

I

Q

Q

V

V

F

I

G

A

D

D

S

R

H

R

G

G

N

N

V

T

V

V

H

Y

L

L

F

P

E

E

R

R

D

E

C

C

S

S

L

I

Q

Q

R

R

R

R

H

N

Q

Q

K

K

V

V

I

I

E

E

E

E

A

A

P

P

A

S

P

V

G

L

M

L

D

D

E

A

A

T

T

T

R

R

E

K

A

A

V

M

C

G

A

E

A

E

A

A

V

V

K

A

A

M

A

A

Q

R

A

A

V

V

N

Q

Y

Y

V

V

G

S

A

A

G

G

T

T

V

V

E

E

F

N

I

V

A

V

D

N

A

P

S

Q

E

K

G

-

L

-

R

-

A

-

D

-

R

Q

I

F

W

Y

F

F

M

L

E

E

M

M

N

N

T

T

R

R

L

L

Q

Q

V

V

E

E

H

H

P

P

V

I

T

T

E

E

M

E

V

I

T

T

G

G

Q

V

D

D

L

L

V

V

E

E

W

Q

Q

M

L

L

R

R

V

A

A

A

S

A

G

D

E

L

P

P

L

L

P

S

L

I

E

T

Q

Q

D

D

E

D

I

I

T

T

L

I

D

N

G

G

W

H

A

A

M

T

E

E

A

C

R

R

L

V

Y

Y

A

A

E

E

N

D

P

P

A

M

T

K

G

N

F

Y

L

F

P

P

S

S

T

I

G

G

K

R

L

L

K

T

H

M

F

Y

R

Q

L

E

P

P

E

T

G

G

-

A

D

G

V

V

R

R

V

C

D

D

S

S

A

G

V

I

E

I

E

E

G

G

G

S

E

Q

V

I

T

S

P

V

F

Y

Y

Y

D

D

P

P

M

L

I

I

A

C

K

K

L

L

I

S

A

T

H

W

G

G

A

R

D

D

R

R

E

A

D

E

A

C

A

I

Q

G

R

R

L

M

A

E

E

K

A

A

C

L

A

D

L

E

V

Y

E

V

V

I

W

R

P

G

V

L

K

R

T

H

N

N

A

I

A

C

F

L

L

L

A

R

K

D

C

V

A

V

S

T

H

E

P

P

D

R

F

Y

V

R

D

S

G

G

A

S

V

I

D

T

T

T

G

N

F

Y

I

-

E

-

A

-

R

-

L

L

D

Q

E

E

L

Q

T

Y

E

P

R

N

A

G

F

F

S

K

D

K

E

-

P

-

A

-

M

-

A

-

A

-

I

-

G

-

W

-

R

-

L

-

D

-

S

A

F

E

L

L

E

T

A

A

E

E

A

E

R

M

R

Q

D

L

P

M

W

Y

E

E

G

V

A

A

P

A

S

C

K

-

L

-

L

-

G

-

F

-

R

-

M

V

N

H

A

L

P

K

R

R

A

E

S

R

M

L

H

H

L

Y

P

T

M

Q

S

G

T

T

D

A

G

P

K

S

A

E

T

R

P

Q

L

L

R

Y

V

L

A

S

L

V

I

G

G

A

G

G

G

Q

T

E

E

G

D

E

W

T

S

P

W

V

D

Y

I

V

R

R

H

Y

A

L

D

D

G

D

S

S

T

H

F

F

D

E

-

I

-

G

-

A

-

S

-

K

-

H

-

G

-

P

-

F

-

R

-

K

-

M

-

E

-

V

-

V

-

W

-

K

-

A

-

S

-

Y

E

P

V

I

T

I

R

R

L

V

P

K

T

D

T

G

Y

E

G

A

K

E

G

T

P

V

I

L

Q

Q

V

F

F

W

E

G

G

T

G

N

D

E

V

V

Q

T

-

Y

-

G

-

M

-

Q

-

M

-

R

-

G

-

T

E

T

F

F

D

D

F

V

-

N

-

V

-

M

-

S

-

D

-

L

-

Q

-

S

-

T

V

L

A

A

K

H

I

F

G

V

G

P

A

I

G

T

E

E

G

A

G

T

A

T

S

N

D

T

G

K

A

Q

I

I

L

L

S

S

P

P

M

M

P

P

G

G

K

V

I

I

V

V

S

A

V

I

S

K

V

V

S

Q

A

P

G

G

Q

Q

T

M

V

V

S

V

K

A

G

G

Q

E

T

E

L

L

L

L

T

T

L

L

E

E

A

A

M
|
M

K
|
K

M

M

E

R

H

N

A

K

M

I

A

H

A

A

P

Q

F

A

D

D

G

G

V

K

V

V

A

K

E

E

L

V

S

K

A

V

V

K

A

L

G

G

G

A

Q

T

V

V

S

E

E

D

G

N

V

E

V

V

L

L

A

V

R

E

L

L

E

E

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: M622 (= M615), K623 (= K616)

4mv4A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and mg2 (see paper)
48% identity, 69% coverage: 1:449/654 of query aligns to 1:441/442 of 4mv4A

query
sites
4mv4A

M

M

L

L

S

E

S

K

V

V

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

R

L

R

R

I

I

I

L

R

R

T

A

A

C

R

K

E

E

L

L

G

G

V

I

R

K

T

T

I

V

A

A

V

V

Y

H

S

S

E

T

A

A

D

D

A

R

N

D

A

L

P

K

F

H

V

V

M

L

E

L

A

A

D

D

A

E

A

T

I

I

L

C

I

I

G

G

P

P

A

A

P

P

A

S

K

A

E

K

S

S

Y

Y

L

L

D

N

P

I

R

P

K

A

I

I

L

I

A

A

A

A

A

A

R

E

Q

V

M

T

G

G

A

A

E

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

A

E

D

F

F

A

A

Q

E

S

Q

V

V

I

E

D

R

A

S

G

G

L

F

V

T

W

F

I

I

G

G

P

P

P

T

P

A

S

D

A

V

I

I

R

R

A

L

M

M

G

G

L

D

K
|
K

D

V

A

S

A

A

K

I

A

K

V

A

M

M

I

K

K

K

A

A

G

G

V

V

P

P

T

C

T

V

P

P

G

G

Y

S

L

D

G

G

E

P

-

V

D

S

Q

N

S

D

V

I

E

A

R

K

L

N

T

K

V

E

E

I

A

A

A

K

K

R

I

I

G

G

Y

Y

P

P

V

I

L
x
I

I

I

K
|
K

A

A

V

S

A

G

G

G

G
|
G

G

-

G

-

K

-

G

G

M
|
M

R

R

K

V

V

V

E

R

R

S

A

E

E

D

D

A

F

L

E

E

A

E

A

S

L

I

G

A

S

M

C

T

R

K

R

A

E

E

A

A

S

K

A

A

A

A

F

F

G

N

D

N

D
|
D

R

M

V

V

L

Y

L

M

E
|
E

K
|
K

Y
|
Y

V
x
L

T

E

R

N

P

P

R

R

H
|
H

I

V

E

E

V

I

Q

Q

V

V

F

L

G

A

D

D

S

T

H

H

G

G

N

N

V

A

V

V

H

Y

L

L

F

A

E

E

R

R

D

D

C

C

S

S

L

M

Q

Q

R

R

R
|
R

H
|
H

Q

Q

K

K

V

V

I

V

E

E

E

E

A

A

P

P

A

A

P

P

G

G

M

I

D

T

E

E

A

E

T

V

R

R

E

R

A

D

V

I

C

G

A

S

A

R

A

C

V

A

K

N

A

A

A

C

Q

V

A

E

V

I

N

G

Y

Y

V

R

G

G

A

A

G

G

T
|
T

V

F

E
|
E

F

F

I
x
L

A

Y

D

E

A

N

S

G

E

E

G

-

L

-

R

-

A

-

D

-

R

-

I

F

W

Y

F

F

M

I

E
|
E

M

M

N
|
N

T

T

R
|
R

L

I

Q

Q

V

V

E
|
E

H

H

P

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

Q

V

D

D

L

L

V

V

E

K

W

E

Q

Q

L

L

R

R

V

I

A

A

S

A

G

G

E

L

P

P

L

I

P

S

L

F

E

K

Q

Q

D

E

E

D

I

I

T

K

L

V

D

K

G

G

W

H

A

A

M

M

E

E

A

C

R
|
R

L

I

Y

N

A

A

E

E

N

D

P

P

A

K

T

T

G

-

F

F

L

L

P

P

S

S

T

P

G

G

K

K

L

V

K

N

H

H

F

L

R

H

L

S

P

P

E

G

G

G

-

L

D

G

V

V

R

R

V

W

D

D

S

S

A

H

V

V

E

Y

E

G

G

G

G

Y

E

T

V

V

T

P

P

P

F

H

Y

Y

D

D

P

S

M

M

I

I

A

A

K

K

L

L

I

I

A

T

H

Y

G

G

A

D

D

T

R

R

E

E

D

V

A

A

A

I

Q

R

R

R

L

M

A

Q

E

N

A

A

C

L

A

S

L

E

V

T

E

I

V

I

W

D

P

G

V

I

K

K

T

T

N

N

A

I

A

P

F

L

L

H

A

E

K

L

C

I

A

L

S

E

H

D

P

E

D

N

F

F

V

Q

D

K

G

G

A

G

V

T

D

N

T

I

G

H

F

Y

I

L

E

E

A

K

R

K

L

L

active site: K116 (= K116), K159 (= K158), D193 (= D195), H206 (= H208), R232 (= R234), T271 (= T273), E273 (= E275), E285 (= E293), N287 (= N295), R289 (= R297), E293 (= E301), R335 (= R343)
binding phosphomethylphosphonic acid adenylate ester: I157 (≠ L156),