SitesBLAST

2:32 mutation Missing: Constitutively active and results in reduced lin-28 protein levels. Rescues the seam cell number and vulval developmental defects of the lin-28 n719 loss of function mutant.
11 D→A: Reduced ced-3-mediated cleavage in vitro.
23 E→A: Reduced ced-3-mediated cleavage in vitro.
24 D→A: Reduced ced-3-mediated cleavage in vitro.
31 D→A: Loss of ced-3-mediated cleavage in vitro. Partially prevents reduction of lin-28 protein levels in vivo. About 20 percent of mutants fail to reach adulthood. Defects in alae morphology.
31:32 Cleavage; by ced-3
32 N→M: Loss of ced-3-mediated cleavage in vitro. Prevents reduction of lin-28 protein levels in vitro.
128 G→E: In ma157.; G→R: In sy283.
133 P→S: In ve9.

Q9Y2V2 Calcium-regulated heat-stable protein 1; Calcium-regulated heat-stable protein of 24 kDa; CRHSP-24 from Homo sapiens (Human) (see 2 papers)
50% identity, 72% coverage: 3:52/69 of query aligns to 64:111/147 of Q9Y2V2

query
sites
Q9Y2V2

N

K

G

G

V

V

V

C

K

K

W

C

F

F

N

C

P

R

A

S

K

K

G

G

F
x
H

G

G

F

F

I

I

Q

T

P

P

E

A

D

D

G

G

Q

P

D

D

V

I

F

F

V

L

H

H

I

I

A

S

A

D

V

V

E

E

R

-

S

-

G

G

L

E

S

Y

G

V

L

P

N

V

E

E

G

G

D

D

Q

E

V

V

A

T

Y

Y

E

K

L

M

H76 (≠ F15) mutation to Q: Reduced affinity for single-stranded DNA.

Sites not aligning to the query:

30 modified: Phosphoserine
32 modified: Phosphoserine
41 modified: Phosphoserine; S→D: Reduced affinity for single-stranded DNA. Abolishes location at cytoplasmic stress granules.
52 modified: Phosphoserine

Q9WU49 Calcium-regulated heat stable protein 1; Calcium-regulated heat-stable protein of 24 kDa; CRHSP-24 from Rattus norvegicus (Rat) (see paper)
50% identity, 72% coverage: 3:52/69 of query aligns to 64:111/147 of Q9WU49

query
sites
Q9WU49

N

K

G

G

V

V

V

C

K

K

W

C

F

F

N

C

P

R

A

S

K

K

G

G

F

H

G

G

F

F

I

I

Q

T

P

P

E

A

D

D

G

G

Q

P

D

D

V

I

F

F

V

L

H

H

I

I

A

S

A

D

V

V

E

E

R

-

S

-

G

G

L

E

S

Y

G

V

L

P

N

V

E

E

G

G

D

D

Q

E

V

V

A

T

Y

Y

E

K

L

M

Sites not aligning to the query:

30 modified: Phosphoserine
32 modified: Phosphoserine
52 modified: Phosphoserine

7f3iA Crystal structure of human ybx2 csd in complex with m5c RNA in space group p212121
40% identity, 74% coverage: 4:54/69 of query aligns to 12:66/93 of 7f3iA

query
sites
7f3iA

G

G

V

T

V

V

K
|
K

W
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W

F

F

N
|
N

P
x
V

A
x
R

K
x
N

G

G

F
x
Y

G

G

F
|
F

I

I

Q

N

P

R

E

N

D

D

G

T

G

K

Q

E

D
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D

V

V

F
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F

V

V

H
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H

I

Q

A

T

A

A

V

I

E

K

R

R

S

N

G

N

-

P

-

R

-

K

-

F

L

L

S

R

G

S

L

V

N

G

E

D

G

G

D

E

Q

T

V

V

A

E

Y

F

E

D

L

V

E

V

E

E

binding : K15 (= K7), W16 (= W8), N18 (= N10), V19 (≠ P11), R20 (≠ A12), N21 (≠ K13), Y23 (≠ F15), F25 (= F17), D34 (= D26), F36 (= F28), H38 (= H30)

Sites not aligning to the query:

binding : 69, 70, 89

Q9VVA0 Cold shock domain-containing protein CG9705 from Drosophila melanogaster (Fruit fly) (see 2 papers)
44% identity, 75% coverage: 1:52/69 of query aligns to 54:103/143 of Q9VVA0

query
sites
Q9VVA0

M

V

A

V

N

T

G

G

V

M

V

V

K

K

W

S

F

F

N

S

P

R

A

T

K

K

G

G

F

H

G

G

F

F

I

I

Q

T

P

P

E

N

D

A

G

G

Q

E

D

D

V

V

F

F

V

C

H

H

I

V

A

S

A

D

V

I

E

E

R

-

S

-

G

G

L

E

S

Y

G

V

L

P

N

M

E

P

G

G

D

D

Q

E

V

V

A

K

Y

Y

E

R

L

L

Sites not aligning to the query:

22 modified: Phosphoserine
24 modified: Phosphoserine
28 modified: Phosphoserine
33 modified: Phosphoserine
139 modified: Phosphoserine
140 modified: Phosphoserine

6a6jA Crystal structure of zebra fish y-box protein1 (yb-1) cold-shock domain in complex with 6mer m5c RNA (see paper)
40% identity, 74% coverage: 4:54/69 of query aligns to 9:63/90 of 6a6jA

query
sites
6a6jA

G

G

V

T

V

V

K

K

W
|
W

F

F

N
|
N

P

V

A

R

K

N

G

G

F

Y

G

G

F
|
F

I

I

Q

N

P

R

E

N

D

D

G

T

G

K

Q

E

D

D

V

V

F
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F

V

V

H
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H

I

Q

A

T

A

A

V

I

E

K

-

K

-

N

-

P

R

R

S

K

G

Y

L

L

S

R

G

S

L

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N

G

E

D

G

G

D

E

Q

T

V

V

A

E

Y

F

E

D

L

V

E

V

E

E

binding : W13 (= W8), N15 (= N10), F22 (= F17), F33 (= F28), H35 (= H30)

Sites not aligning to the query:

binding zinc ion: 65, 69
binding : 65, 66, 67, 68

6lmrA Solution structure of cold shock domain and ssdna complex (see paper)
40% identity, 74% coverage: 4:54/69 of query aligns to 11:65/90 of 6lmrA

query
sites
6lmrA

G

G

V

T

V

V

K

K

W
|
W

F

F

N
|
N

P

V

A
x
R

K

N

G

G

F

Y

G

G

F
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F

I

I

Q

N

P

R

E

N

D

D

G

T

G

K

Q

E

D

D

V

V

F
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F

V

V

H
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H

I

Q

A

T

A

A

V

I

E

K

-

K

-

N

-

P

R

R

S

K

G

Y

L

L

S

R

G

S

L

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N

G

E

D

G

G

D

E

Q

T

V

V

A

E

Y

F

E

D

L

V

E

V

E

E

binding : W15 (= W8), N17 (= N10), R19 (≠ A12), F24 (= F17), F35 (= F28), H37 (= H30)

Sites not aligning to the query:

binding : 67, 68, 71, 87, 88

Q9Z2C8 Y-box-binding protein 2; FRGY2 homolog; Germ cell-specific Y-box-binding protein from Mus musculus (Mouse) (see paper)
40% identity, 74% coverage: 4:54/69 of query aligns to 98:152/360 of Q9Z2C8

query
sites
Q9Z2C8

G
|
G

V
x
T

V
|
V

K
|
K

W
|
W

F
|
F

N
|
N

P
x
V

A
x
R

K
x
N

G
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G

F
x
Y

G
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G

F
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F

I
|
I

Q
x
N

P
x
R

E
x
N

D
|
D

G
x
T

G
x
K

Q
x
E

D
|
D

V
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V

F
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F

V
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V

H
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H

I
x
Q

A
x
T

A
|
A

V
x
I

E
x
K

R
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R

S
x
N

G
x
N

-
x
P

-
x
R

-
x
K

-
x
F

L
|
L

S
x
R

G
x
S

L
x
V

N
x
G

E
x
D

G
|
G

D
x
E

Q
x
T

V
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V

A
x
E

Y
x
F

E
x
D

L
x
V

E
x
V

E
|
E

Y109 (≠ F15) mutation to A: Abolishes cytoplasmic retention and mRNA-binding affinity; when associated with A-111.
F111 (= F17) mutation to A: Abolishes cytoplasmic retention and mRNA-binding affinity; when associated with A-109.

Sites not aligning to the query:

89:171 Required for cytoplasmic retention
219:360 Required for mRNA-binding

B5DE31 Y-box-binding protein 1; YB-1; ZfY1; Y-box transcription factor from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
40% identity, 71% coverage: 4:52/69 of query aligns to 41:93/310 of B5DE31

query
sites
B5DE31

G

G

V

T

V

V

K

K

W
|
W

F
|
F

N
|
N

P
x
V

A
x
R

K
x
N

G

G

F

Y

G

G

F

F

I

I

Q

N

P

R

E

N

D

D

G

T

G

K

Q

E

D

D

V

V

F

F

V

V

H

H

I

Q

A

T

A

A

V

I

E

K

-

K

-

N

-

P

R

R

S

K

G

Y

L

L

S

R

G

S

L

V

N

G

E

D

G

G

D

E

Q

T

V

V

A

E

Y

F

E

D

L

V

W45 (= W8) Important for C5-methylcytosine-recognition; mutation to A: Abolished binding to C5-methylcytosine (m5C)-containing mRNAs.; mutation to F: Decreased binding to C5-methylcytosine (m5C)-containing mRNAs. Decreased ability to discriminate between m5C-containing and unmethylated mRNAs.
WFNVRN 45:50 (≠ WFNPAK 8:13) C5-methylcytosine binding

Q28618 Y-box-binding protein 1; YB-1; Nuclease-sensitive element-binding protein 1; Y-box transcription factor; p50 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
38% identity, 71% coverage: 4:52/69 of query aligns to 61:113/324 of Q28618

query
sites
Q28618

G

G

V

T

V

V

K

K

W

W

F

F

N

N

P

V

A

R

K

N

G

G

F

Y

G

G

F

F

I

I

Q

N

P

R

E

N

D

D

G

T

G

K

Q

E

D

D

V

V

F

F

V

V

H

H

I

Q

A

T

A

A

V

I

E

K

R

K

S

N

G

N

-

P

-

R

-

K

-

Y

L

L

S

R

G
x
S

L

V

N

G

E

D

G

G

D

E

Q

T

V

V

A

E

Y

F

E

D

L

V

S102 (≠ G41) modified: Phosphoserine; by PKB/AKT1; mutation to A: Abolishes phosphorylation by PKB/AKT1.

Sites not aligning to the query:

219:220 Cleavage; by 20S proteasomal protease

P67809 Y-box-binding protein 1; YB-1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Nuclease-sensitive element-binding protein 1; Y-box transcription factor from Homo sapiens (Human) (see 5 papers)
38% identity, 71% coverage: 4:52/69 of query aligns to 61:113/324 of P67809

query
sites
P67809

G
|
G

V
x
T

V
|
V

K
|
K

W
|
W

F
|
F

N
|
N

P
x
V

A
x
R

K
x
N

G
|
G

F

Y

G

G

F

F

I

I

Q

N

P

R

E

N

D

D

G

T

G

K

Q

E

D

D

V

V

F

F

V

V

H

H

I

Q

A

T

A

A

V

I

E

K

R

K

S

N

G

N

-

P

-

R

-

K

-

Y

L

L

S

R

G
x
S

L

V

N

G

E

D

G

G

D

E

Q

T

V

V

A

E

Y

F

E

D

L

V

W65 (= W8) Important for C5-methylcytosine-recognition; mutation to A: Abolished binding to C5-methylcytosine (m5C)-containing mRNAs.; mutation to F: Decreased binding to C5-methylcytosine (m5C)-containing mRNAs. Decreased ability to discriminate between m5C-containing and unmethylated mRNAs.
WFNVRN 65:70 (≠ WFNPAK 8:13) C5-methylcytosine binding
S102 (≠ G41) modified: Phosphoserine; by PKB/AKT1; mutation to A: Loss of phosphorylation by PKB/AKT1. Inhibits translocation to the nucleus and tumor cell growth.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine
15:71 Interaction with ss-DNA
137 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
165 modified: Phosphoserine
301 modified: N6-acetyllysine; K→A: Abrogates unconventional secretion.
304 modified: N6-acetyllysine; K→A: Abrogates unconventional secretion.

Query Sequence

>CCNA_02705
MANGVVKWFNPAKGFGFIQPEDGGQDVFVHIAAVERSGLSGLNEGDQVAYELEEDRRSGK
TSAGNLRIL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory