SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Dsui_3224 FitnessBrowser__PS:Dsui_3224 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

P61459 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Rattus norvegicus (Rat) (see 2 papers)
39% identity, 86% coverage: 14:109/111 of query aligns to 1:100/104 of P61459

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M1 (≠ L14) modified: Initiator methionine, Removed
A2 (≠ S15) modified: N-acetylalanine
C82 (≠ V91) mutation to R: Reduced dehydratase activity. No impact on hydroxytetrahydrobiopterin-binding.

P61457 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Homo sapiens (Human) (see 6 papers)
39% identity, 86% coverage: 14:109/111 of query aligns to 1:100/104 of P61457

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M1 (≠ L14) modified: Initiator methionine, Removed
A2 (≠ S15) modified: N-acetylalanine
T79 (= T88) to I: in HPABH4D; increased proteolytic degradation; reduced interaction with HNF1B; loss of HNF1B-coactivator activity; dbSNP:rs121913014
C82 (≠ V91) to R: in HPABH4D; increased proteolytic degradation; reduced dehydratase activity; no impact on hydroxytetrahydrobiopterin-binding; reduced interaction with HNF1B; partial impact on HNF1B-coactivator activity; dbSNP:rs104894177
R88 (≠ N97) to Q: in HPABH4D; when associated in cis with 27-E--T-104 del; uncertain significance; no impact on HNF1B interaction; no impact on HNF1B-coactivator activity; dbSNP:rs115117837
E97 (= E106) to K: in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs397518416

Sites not aligning to the query:

27:104 natural variant: Missing (in HPABH4D; when associated in cis with Q-88; reduced protein levels; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs727505360)
87:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs104894172)
98:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs121913015)

Q02I31 Putative pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
31% identity, 94% coverage: 6:109/111 of query aligns to 4:111/118 of Q02I31

query
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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: Phosphothreonine

1f93A Crystal structure of a complex between the dimerization domain of hnf-1 alpha and the coactivator dcoh (see paper)
39% identity, 86% coverage: 15:109/111 of query aligns to 1:99/103 of 1f93A

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active site: E57 (≠ Q67), H61 (= H71), H62 (= H72), H79 (= H89), E80 (≠ D90), D88 (= D98)
binding : N43 (≠ E53), R44 (≠ A54), G47 (≠ L57), R51 (≠ G61), L54 (≠ W64)

1dcpA Dcoh, a bifunctional protein-binding transcriptional coactivator, complexed with biopterin (see paper)
40% identity, 83% coverage: 19:110/111 of query aligns to 1:96/99 of 1dcpA

query
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1dcpA

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active site: E53 (≠ Q67), H57 (= H71), H58 (= H72), H75 (= H89), E76 (≠ D90), D84 (= D98)
binding 7,8-dihydrobiopterin: D56 (≠ N70), H57 (= H71), H58 (= H72), T74 (= T88), H75 (= H89)

2v6tB Crystal structure of a complex of pterin-4a-carbinolamine dehydratase from toxoplasma gondii with 7,8-dihydrobiopterin (see paper)
40% identity, 71% coverage: 24:102/111 of query aligns to 6:89/100 of 2v6tB

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active site: D54 (≠ Q67), H58 (= H71), H59 (= H72), H76 (= H89), D77 (= D90), D85 (= D98)
binding 2-amino-6-(1,2-dihydroxy-propyl)-7,8-dihydro-6h-pteridin-4-one: D57 (≠ N70), H58 (= H71), H59 (= H72), D77 (= D90)

Query Sequence

>Dsui_3224 FitnessBrowser__PS:Dsui_3224
MLLDDLARDTCRHLSGPQHRLDDATVAMLLPLLPGWSREDGHIAKTFHFTGYEAAMLFAN
GVAWLAQRQNHHPELQVNYNTVRVTYGTHDVGGLSRNDFICAAKIEALAAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory