SitesBLAST – Find functional sites

 

SitesBLAST

Comparing EX31_RS25170 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8rd8Ba Small ribosomal subunit protein uS5 (see paper)
84% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 8rd8Ba

query
sites
8rd8Ba
M
|
M
K
|
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
|
P
S
|
S
V
|
V
L
x
I
K
|
K
R
|
R
N
x
K
R
|
R
S
 
T
H
|
H
G
 
G
F
|
F
R
|
R
A
 
A
R
|
R
M
|
M
A
 
A
T
 
T
K
|
K
N
x
K
G
 
G
R
 
R
Q
|
Q
V
 
V
L
 
L
A
|
A
R
|
R
R
|
R
R
|
R
A
 
A
K
|
K
G
|
G
R
|
R
T
x
H
R
|
R
L
 
L
S
 
T
A
 
V

8cd14 phikz014 (see paper)
84% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 8cd14

query
sites
8cd14
M
|
M
K
|
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
 
P
S
|
S
V
x
T
L
|
L
K
|
K
R
|
R
N
x
A
R
|
R
S
x
V
H
|
H
G
 
G
F
|
F
R
|
R
A
 
A
R
|
R
M
|
M
A
 
A
T
 
T
K
 
K
N
|
N
G
 
G
R
 
R
Q
 
Q
V
|
V
L
 
L
A
 
S
R
|
R
R
|
R
R
|
R
A
 
A
K
|
K
G
|
G
R
|
R
T
x
K
R
|
R
L
 
L
S
x
T
A
x
V

7unw6 50S ribosomal protein L34
84% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 7unw6

query
sites
7unw6
M
|
M
K
 
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
 
P
S
|
S
V
x
T
L
|
L
K
|
K
R
|
R
N
x
A
R
|
R
S
 
V
H
|
H
G
 
G
F
|
F
R
|
R
A
 
A
R
|
R
M
 
M
A
 
A
T
 
T
K
|
K
N
|
N
G
 
G
R
 
R
Q
 
Q
V
 
V
L
 
L
A
x
S
R
|
R
R
|
R
R
|
R
A
 
A
K
|
K
G
|
G
R
|
R
T
x
K
R
|
R
L
 
L
S
 
T
A
 
V

7m4v1 A. Baumannii ribosome-eravacycline complex: 50s (see paper)
82% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 7m4v1

query
sites
7m4v1
M
|
M
K
|
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
 
P
S
|
S
V
x
E
L
 
L
K
|
K
R
|
R
N
x
K
R
|
R
S
 
V
H
|
H
G
 
G
F
|
F
R
|
R
A
 
A
R
|
R
M
 
M
A
 
A
T
 
T
K
|
K
N
x
A
G
 
G
R
 
R
Q
|
Q
V
 
V
L
 
L
A
 
A
R
|
R
R
|
R
R
|
R
A
 
A
K
|
K
G
|
G
R
|
R
T
x
H
R
 
S
L
 
L
S
 
T
A
 
V

8a3l1 50S ribosomal protein L36 (see paper)
93% identity, 100% coverage: 1:46/46 of query aligns to 1:46/46 of 8a3l1

query
sites
8a3l1
M
|
M
K
|
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
|
P
S
|
S
V
|
V
L
|
L
K
|
K
R
|
R
N
|
N
R
|
R
S
 
S
H
|
H
G
 
G
F
|
F
R
|
R
A
 
A
R
|
R
M
 
M
A
 
A
T
 
T
K
|
K
N
|
N
G
 
G
R
 
R
Q
 
Q
V
 
V
L
 
L
A
 
A
R
|
R
R
|
R
R
|
R
A
 
A
K
|
K
G
|
G
R
|
R
T
x
A
R
|
R
L
 
L
S
 
T
A
 
V
S
|
S
K
|
K

3bbx2 The hsp15 protein fitted into the low resolution cryo-em map of the 50s.Nc-tRNA.Hsp15 complex (see paper)
93% identity, 100% coverage: 1:46/46 of query aligns to 1:46/46 of 3bbx2

query
sites
3bbx2
M
|
M
K
|
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
 
P
S
|
S
V
|
V
L
|
L
K
|
K
R
|
R
N
 
N
R
 
R
S
|
S
H
|
H
G
|
G
F
 
F
R
|
R
A
 
A
R
|
R
M
 
M
A
 
A
T
|
T
K
 
K
N
|
N
G
|
G
R
 
R
Q
 
Q
V
|
V
L
 
L
A
 
A
R
 
R
R
|
R
R
 
R
A
 
A
K
 
K
G
|
G
R
|
R
T
x
A
R
|
R
L
|
L
S
x
T
A
x
V
S
 
S
K
 
K

7a0r2 50s deinococcus radiodurans ribosome bounded with mycinamicin i (see paper)
67% identity, 98% coverage: 1:45/46 of query aligns to 1:45/47 of 7a0r2

query
sites
7a0r2
M
|
M
K
|
K
R
|
R
T
|
T
F
x
Y
Q
|
Q
P
|
P
S
x
N
V
x
N
L
x
R
K
|
K
R
|
R
N
x
A
R
x
K
S
 
T
H
|
H
G
|
G
F
|
F
R
|
R
A
 
A
R
|
R
M
|
M
A
 
K
T
 
T
K
 
K
N
x
S
G
 
G
R
 
R
Q
 
N
V
x
I
L
 
L
A
 
A
R
|
R
R
 
R
R
|
R
A
 
A
K
|
K
G
|
G
R
|
R
T
x
H
R
 
Q
L
|
L
S
 
T
A
 
V
S
|
S

Sites not aligning to the query:

3j3v2 Atomic model of the immature 50s subunit from bacillus subtilis (state i-a) (see paper)
70% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 3j3v2

query
sites
3j3v2
M
|
M
K
|
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
 
P
S
x
N
V
x
N
L
x
R
K
|
K
R
|
R
N
 
S
R
x
K
S
x
V
H
|
H
G
|
G
F
|
F
R
|
R
A
x
S
R
 
R
M
|
M
A
 
S
T
 
S
K
|
K
N
 
N
G
 
G
R
|
R
Q
 
L
V
 
V
L
 
L
A
 
A
R
|
R
R
|
R
R
|
R
A
x
R
K
|
K
G
|
G
R
|
R
T
x
K
R
x
V
L
 
L
S
 
S
A
|
A

8uua6 Cryo-em structure of the listeria innocua 50s ribosomal subunit in complex with hflxr (structure iii) (see paper)
70% identity, 93% coverage: 1:43/46 of query aligns to 1:43/43 of 8uua6

query
sites
8uua6
M
|
M
K
 
K
R
|
R
T
|
T
F
x
Y
Q
|
Q
P
|
P
S
|
S
V
x
K
L
x
R
K
|
K
R
|
R
N
x
K
R
x
K
S
 
V
H
|
H
G
 
G
F
|
F
R
|
R
A
 
T
R
|
R
M
 
M
A
 
S
T
 
T
K
|
K
N
|
N
G
 
G
R
 
R
Q
x
R
V
 
V
L
 
L
A
 
A
R
 
S
R
|
R
R
|
R
A
 
R
K
|
K
G
|
G
R
|
R
T
 
K
R
 
V
L
 
L
S
 
S

6dddP Structure of the 50s ribosomal subunit from methicillin resistant staphylococcus aureus in complex with the oxazolidinone antibiotic lzd-5 (see paper)
66% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 6dddP

query
sites
6dddP
M
x
V
K
 
K
R
|
R
T
|
T
F
x
Y
Q
|
Q
P
|
P
S
x
N
V
x
K
L
x
R
K
|
K
R
x
H
N
x
S
R
 
K
S
 
V
H
|
H
G
 
G
F
|
F
R
|
R
A
 
K
R
|
R
M
 
M
A
 
S
T
 
T
K
|
K
N
|
N
G
 
G
R
 
R
Q
x
K
V
 
V
L
 
L
A
 
A
R
|
R
R
|
R
R
 
R
A
x
R
K
|
K
G
|
G
R
|
R
T
x
K
R
 
V
L
 
L
S
 
S
A
 
A

6o8w4 30S ribosomal protein S10 (see paper)
66% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 6o8w4

query
sites
6o8w4
M
|
M
K
 
K
R
|
R
T
|
T
F
x
Y
Q
|
Q
P
|
P
S
x
N
V
x
K
L
x
R
K
|
K
R
|
R
N
x
Q
R
x
K
S
 
V
H
|
H
G
 
G
F
|
F
R
|
R
A
 
K
R
|
R
M
 
M
A
 
S
T
 
T
K
|
K
N
|
N
G
 
G
R
 
R
Q
x
R
V
 
V
L
 
L
A
 
A
R
 
S
R
|
R
R
|
R
A
x
R
K
|
K
G
 
G
R
|
R
T
 
K
R
 
V
L
 
I
S
 
S
A
 
A

9c4g1 Cutibacterium acnes 50s ribosomal subunit with clindamycin bound (see paper)
66% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 9c4g1

query
sites
9c4g1
M
|
M
K
|
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
|
P
S
|
S
V
x
N
L
x
R
K
x
R
R
|
R
N
x
A
R
|
R
S
x
N
H
|
H
G
|
G
F
|
F
R
|
R
A
 
S
R
|
R
M
 
M
A
 
S
T
 
T
K
x
R
N
x
A
G
 
G
R
 
R
Q
 
S
V
 
I
L
 
L
A
 
A
R
 
A
R
|
R
R
|
R
A
x
R
K
|
K
G
|
G
R
|
R
T
x
V
R
 
N
L
 
L
S
 
S
A
 
A

8crx1 30S ribosomal protein S17 (see paper)
66% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 8crx1

query
sites
8crx1
M
|
M
K
|
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
|
P
S
|
S
V
x
N
L
x
R
K
x
R
R
|
R
N
x
A
R
|
R
S
x
N
H
|
H
G
|
G
F
|
F
R
|
R
A
 
S
R
|
R
M
 
M
A
 
S
T
 
T
K
x
R
N
x
A
G
 
G
R
 
R
Q
 
S
V
 
I
L
 
L
A
 
A
R
 
A
R
|
R
R
|
R
A
x
R
K
|
K
G
|
G
R
|
R
T
x
V
R
 
N
L
 
L
S
 
S
A
 
A

8p7x0 50S ribosomal protein L29 (see paper)
62% identity, 98% coverage: 1:45/46 of query aligns to 1:45/48 of 8p7x0

query
sites
8p7x0
M
|
M
K
|
K
R
|
R
T
|
T
F
x
Y
Q
|
Q
P
|
P
S
|
S
V
x
K
L
 
L
K
|
K
R
|
R
N
 
A
R
 
K
S
x
T
H
|
H
G
|
G
F
|
F
R
x
L
A
 
A
R
|
R
M
 
M
A
 
A
T
 
T
K
 
A
N
x
S
G
 
G
R
 
R
Q
 
K
V
 
V
L
 
L
A
x
K
R
 
L
R
|
R
R
|
R
A
x
K
K
|
K
G
x
Q
R
|
R
T
x
A
R
 
Q
L
 
L
S
 
T
A
 
V
S
|
S

Sites not aligning to the query:

5a9zAd of Thermous thermophilus ribosome bound to BipA-GDPCP (see paper)
62% identity, 98% coverage: 1:45/46 of query aligns to 1:45/49 of 5a9zAd

query
sites
5a9zAd
M
|
M
K
|
K
R
|
R
T
|
T
F
x
W
Q
|
Q
P
|
P
S
x
N
V
x
R
L
x
R
K
|
K
R
|
R
N
x
A
R
x
K
S
 
T
H
|
H
G
|
G
F
|
F
R
|
R
A
 
A
R
 
R
M
|
M
A
x
R
T
 
T
K
 
P
N
 
G
G
 
G
R
|
R
Q
x
K
V
|
V
L
 
L
A
x
K
R
|
R
R
|
R
R
 
R
A
 
Q
K
 
K
G
|
G
R
|
R
T
x
W
R
 
R
L
 
L
S
 
T
A
 
P
S
 
A

Sites not aligning to the query:

1vy4B7 50S ribosomal protein L36 (see paper)
62% identity, 98% coverage: 1:45/46 of query aligns to 1:45/48 of 1vy4B7

query
sites
1vy4B7
M
|
M
K
|
K
R
|
R
T
|
T
F
x
W
Q
|
Q
P
|
P
S
x
N
V
x
R
L
x
R
K
|
K
R
|
R
N
x
A
R
x
K
S
 
T
H
|
H
G
|
G
F
|
F
R
|
R
A
|
A
R
|
R
M
|
M
A
 
R
T
 
T
K
x
P
N
x
G
G
 
G
R
 
R
Q
x
K
V
 
V
L
 
L
A
x
K
R
|
R
R
|
R
R
|
R
A
x
Q
K
|
K
G
|
G
R
|
R
T
x
W
R
|
R
L
 
L
S
 
T
A
 
P
S
 
A

Sites not aligning to the query:

5myjB6 of 70S ribosome from Lactococcus lactis (see paper)
64% identity, 96% coverage: 1:44/46 of query aligns to 1:44/44 of 5myjB6

query
sites
5myjB6
M
|
M
K
 
K
R
|
R
T
|
T
F
x
Y
Q
|
Q
P
|
P
S
x
H
V
x
K
L
x
K
K
x
S
R
|
R
N
x
K
R
 
T
S
 
T
H
|
H
G
|
G
F
|
F
R
|
R
A
 
S
R
|
R
M
 
M
A
 
A
T
 
T
K
|
K
N
|
N
G
 
G
R
|
R
Q
x
R
V
|
V
L
 
L
A
 
A
R
 
A
R
|
R
R
|
R
A
x
R
K
|
K
G
 
G
R
|
R
T
x
A
R
 
S
L
 
L
S
 
T
A
 
V

8c972 Cryo-em captures early ribosome assembly in action (see paper)
100% identity, 59% coverage: 10:36/46 of query aligns to 1:27/32 of 8c972

query
sites
8c972
L
 
L
K
 
K
R
 
R
N
 
N
R
 
R
S
|
S
H
|
H
G
 
G
F
|
F
R
 
R
A
 
A
R
 
R
M
 
M
A
 
A
T
 
T
K
 
K
N
|
N
G
 
G
R
 
R
Q
 
Q
V
 
V
L
 
L
A
 
A
R
|
R
R
|
R
R
|
R
A
 
A

Sites not aligning to the query:

5o60d Structure of the 50s large ribosomal subunit from mycobacterium smegmatis (see paper)
58% identity, 93% coverage: 2:44/46 of query aligns to 4:46/46 of 5o60d

query
sites
5o60d
K
 
K
R
|
R
T
|
T
F
|
F
Q
|
Q
P
|
P
S
x
N
V
x
N
L
x
R
K
x
R
R
|
R
N
x
A
R
|
R
S
 
V
H
|
H
G
|
G
F
|
F
R
|
R
A
 
L
R
|
R
M
 
M
A
 
R
T
 
T
K
x
R
N
 
A
G
 
G
R
 
R
Q
 
A
V
x
I
L
 
V
A
 
A
R
 
N
R
|
R
R
|
R
A
 
S
K
|
K
G
|
G
R
|
R
T
x
R
R
 
A
L
 
L
S
 
T
A
 
A

Sites not aligning to the query:

5mrcY of the yeast mitochondrial ribosome - Class A (see paper)
55% identity, 87% coverage: 4:43/46 of query aligns to 6:45/46 of 5mrcY

query
sites
5mrcY
T
|
T
F
x
Y
Q
|
Q
P
|
P
S
 
S
V
x
T
L
|
L
K
|
K
R
|
R
N
x
K
R
|
R
S
 
T
H
x
F
G
|
G
F
|
F
R
x
L
A
 
A
R
|
R
M
x
A
A
x
K
T
 
S
K
|
K
N
x
Q
G
 
G
R
x
S
Q
x
K
V
x
I
L
 
L
A
 
K
R
|
R
R
|
R
R
 
K
A
x
L
K
|
K
G
|
G
R
|
R
T
x
W
R
 
F
L
 
L
S
 
S

Sites not aligning to the query:

Query Sequence

>EX31_RS25170
MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRTRLSASK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory