SitesBLAST
Comparing Echvi_3219 FitnessBrowser__Cola:Echvi_3219 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6n7lC Crystal structure of an alcohol dehydrogenase from elizabethkingia anophelis nuhp1
72% identity, 99% coverage: 1:341/343 of query aligns to 3:342/344 of 6n7lC
- active site: C45 (= C43), T47 (= T45), H50 (= H48), H68 (= H66), C154 (= C153)
- binding nicotinamide-adenine-dinucleotide: C45 (= C43), H46 (= H44), T47 (= T45), H50 (= H48), C154 (= C153), T158 (= T157), G178 (= G177), G180 (= G179), G181 (= G180), L182 (= L181), D201 (= D200), V202 (= V201), K206 (= K205), T243 (= T242), A244 (= A243), V245 (= V244), S246 (= S245), A249 (= A248), N266 (= N265), G267 (= G266), L268 (= L267), I291 (= I290), V292 (= V291)
- binding zinc ion: C45 (= C43), H68 (= H66), C98 (= C97), C101 (= C100), C104 (= C103), C112 (= C111), C154 (= C153)
1lluA The ternary complex of pseudomonas aeruginosa alcohol dehydrogenase with its coenzyme and weak substrate (see paper)
67% identity, 99% coverage: 2:341/343 of query aligns to 2:341/341 of 1lluA
- active site: C43 (= C43), H44 (= H44), T45 (= T45), H48 (= H48), H66 (= H66), E67 (= E67), C97 (= C97), C100 (= C100), C103 (= C103), C111 (= C111), Q115 (≠ V115), C153 (= C153), T157 (= T157), R336 (= R336)
- binding 1,2-ethanediol: H44 (= H44), T45 (= T45), L47 (= L47), D53 (= D53), W92 (= W92), C153 (= C153)
- binding nicotinamide-adenine-dinucleotide: C43 (= C43), H44 (= H44), T45 (= T45), H48 (= H48), C153 (= C153), T157 (= T157), G179 (= G179), G180 (= G180), L181 (= L181), D200 (= D200), I201 (≠ V201), K205 (= K205), A243 (= A243), V244 (= V244), S245 (= S245), A248 (= A248), V265 (≠ N265), L267 (= L267), I290 (= I290), V291 (= V291), R336 (= R336)
- binding zinc ion: C43 (= C43), H66 (= H66), C100 (= C100), C103 (= C103), C111 (= C111), C153 (= C153)
3s2fE Crystal structure of furx nadh:furfural
66% identity, 98% coverage: 6:341/343 of query aligns to 3:338/340 of 3s2fE
- active site: C40 (= C43), H41 (= H44), T42 (= T45), H45 (= H48), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), Q112 (≠ V115), C150 (= C153), T154 (= T157), R333 (= R336)
- binding furfural: T42 (= T45), W51 (= W54), H63 (= H66), W89 (= W92), C150 (= C153), I287 (= I290)
- binding nicotinamide-adenine-dinucleotide: C40 (= C43), H41 (= H44), T42 (= T45), C150 (= C153), T154 (= T157), G174 (= G177), G176 (= G179), G177 (= G180), L178 (= L181), D197 (= D200), I198 (≠ V201), K202 (= K205), T239 (= T242), A240 (= A243), V241 (= V244), N262 (= N265), G263 (= G266), L264 (= L267), I287 (= I290), V288 (= V291), R333 (= R336)
- binding zinc ion: C40 (= C43), H63 (= H66), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
3s2fA Crystal structure of furx nadh:furfural
66% identity, 98% coverage: 6:341/343 of query aligns to 3:338/340 of 3s2fA
- active site: C40 (= C43), H41 (= H44), T42 (= T45), H45 (= H48), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), Q112 (≠ V115), C150 (= C153), T154 (= T157), R333 (= R336)
- binding phosphorylisopropane: T42 (= T45), H63 (= H66), W89 (= W92), I287 (= I290)
- binding zinc ion: C40 (= C43), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
3s2eE Crystal structure of furx nadh complex 1
66% identity, 98% coverage: 6:341/343 of query aligns to 3:338/340 of 3s2eE
- active site: C40 (= C43), H41 (= H44), T42 (= T45), H45 (= H48), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), Q112 (≠ V115), C150 (= C153), T154 (= T157), R333 (= R336)
- binding nicotinamide-adenine-dinucleotide: C40 (= C43), H41 (= H44), T42 (= T45), C150 (= C153), T154 (= T157), G176 (= G179), G177 (= G180), L178 (= L181), D197 (= D200), I198 (≠ V201), K202 (= K205), T239 (= T242), A240 (= A243), V241 (= V244), S242 (= S245), A245 (= A248), N262 (= N265), G263 (= G266), L264 (= L267), I287 (= I290), V288 (= V291)
- binding zinc ion: C40 (= C43), H63 (= H66), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
3s2eA Crystal structure of furx nadh complex 1
66% identity, 98% coverage: 6:341/343 of query aligns to 3:338/340 of 3s2eA
- active site: C40 (= C43), H41 (= H44), T42 (= T45), H45 (= H48), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), Q112 (≠ V115), C150 (= C153), T154 (= T157), R333 (= R336)
- binding zinc ion: C40 (= C43), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
3meqA Crystal structure of alcohol dehydrogenase from brucella melitensis
66% identity, 99% coverage: 4:341/343 of query aligns to 1:340/341 of 3meqA
- active site: C40 (= C43), H41 (= H44), T42 (= T45), H45 (= H48), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), L112 (≠ V115), C150 (= C153), T154 (= T157), R335 (= R336)
- binding 1,4-dihydronicotinamide adenine dinucleotide: C40 (= C43), H41 (= H44), T42 (= T45), H45 (= H48), C150 (= C153), T154 (= T157), G176 (= G179), G177 (= G180), L178 (= L181), D197 (= D200), I198 (≠ V201), K202 (= K205), T241 (= T242), A242 (= A243), V243 (= V244), S244 (= S245), A247 (= A248), N264 (= N265), G265 (= G266), L266 (= L267), I289 (= I290), V290 (= V291)
- binding zinc ion: C40 (= C43), H63 (= H66), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
Q8GIX7 Alcohol dehydrogenase; ADH; EC 1.1.1.1 from Moraxella sp. (strain TAE123) (see paper)
65% identity, 98% coverage: 6:340/343 of query aligns to 1:335/338 of Q8GIX7
- C38 (= C43) binding
- H61 (= H66) binding
- E62 (= E67) binding
- C92 (= C97) binding
- C95 (= C100) binding
- C98 (= C103) binding
- C106 (= C111) binding
- C148 (= C153) binding
4z6kA Alcohol dehydrogenase from the antarctic psychrophile moraxella sp. Tae 123
65% identity, 98% coverage: 6:340/343 of query aligns to 1:335/345 of 4z6kA
- active site: C38 (= C43), H39 (= H44), T40 (= T45), H43 (= H48), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), Q110 (≠ V115), C148 (= C153), T152 (= T157), R331 (= R336)
- binding zinc ion: C38 (= C43), H61 (= H66), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), C148 (= C153)
6z42A The low resolution structure of a zinc-dependent alcohol dehydrogenase from halomonas elongata.
66% identity, 99% coverage: 4:341/343 of query aligns to 2:335/336 of 6z42A
- active site: C41 (= C43), T43 (= T45), H46 (= H48), H64 (= H66), C148 (= C153)
- binding zinc ion: C41 (= C43), H64 (= H66), E65 (= E67), C95 (= C97), C98 (= C100), C101 (= C103), C109 (= C111), C148 (= C153)
1rjwA Crystal structure of NAD(+)-dependent alcohol dehydrogenase from bacillus stearothermophilus strain lld-r (see paper)
60% identity, 98% coverage: 6:341/343 of query aligns to 1:336/339 of 1rjwA
- active site: C38 (= C43), H39 (= H44), T40 (= T45), H43 (= H48), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), K110 (≠ V115), C148 (= C153), T152 (= T157), R331 (= R336)
- binding trifluoroethanol: T40 (= T45), C148 (= C153), I285 (= I290)
- binding zinc ion: C38 (= C43), H61 (= H66), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111)
P12311 Alcohol dehydrogenase; ADH-T; EC 1.1.1.1 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
60% identity, 98% coverage: 6:341/343 of query aligns to 1:336/337 of P12311
- C38 (= C43) mutation to S: No activity.
- T40 (= T45) mutation to A: No activity.; mutation to S: Little decrease in activity.
- H43 (= H48) mutation to A: No activity.; mutation to R: Higher level of activity at pH 9.
3piiA Crystal structure of mutant of ht- alcohol dehydrogenase with substrate analogue butyramide
60% identity, 98% coverage: 6:341/343 of query aligns to 1:336/337 of 3piiA
- active site: C38 (= C43), H39 (= H44), T40 (= T45), H43 (= H48), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), K110 (≠ V115), C148 (= C153), T152 (= T157), R331 (= R336)
- binding butyramide: T40 (= T45), H61 (= H66), W87 (= W92), C148 (= C153)
- binding zinc ion: C38 (= C43), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), C148 (= C153)
6iqdA Crystal structure of alcohol dehydrogenase from geobacillus stearothermophilus (see paper)
59% identity, 98% coverage: 6:341/343 of query aligns to 1:336/336 of 6iqdA
- active site: C38 (= C43), T40 (= T45), H43 (= H48), H61 (= H66), C148 (= C153)
- binding zinc ion: C38 (= C43), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), C148 (= C153)
4eezB Crystal structure of lactococcus lactis alcohol dehydrogenase variant re1 (see paper)
45% identity, 98% coverage: 6:341/343 of query aligns to 1:338/342 of 4eezB
- active site: C39 (= C43), H40 (= H44), T41 (= T45), H44 (= H48), H60 (= H66), E61 (= E67), C91 (= C97), C94 (= C100), C97 (= C103), C105 (= C111), K109 (≠ V115), C147 (= C153), T151 (= T157), R333 (= R336)
- binding zinc ion: C39 (= C43), H60 (= H66), E61 (= E67), C91 (= C97), C94 (= C100), C97 (= C103), C105 (= C111), C147 (= C153)
P00331 Alcohol dehydrogenase 2; Alcohol dehydrogenase II; ADHII; YADH-2; EC 1.1.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
42% identity, 99% coverage: 2:340/343 of query aligns to 3:345/348 of P00331
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylserine
5yatA Crystal structure of mitochondrial alcohol dehydrogenase isozyme iii from komagataella phaffii gs115 (see paper)
44% identity, 99% coverage: 2:340/343 of query aligns to 2:344/347 of 5yatA
- active site: C43 (= C43), T45 (= T45), H48 (= H48), H66 (= H66), C153 (= C153)
- binding zinc ion: C43 (= C43), H66 (= H66), E67 (= E67), C97 (= C97), C100 (= C100), C103 (= C103), C111 (= C111), C153 (= C153)
5envA Yeast alcohol dehydrogenase with bound coenzyme (see paper)
41% identity, 99% coverage: 2:340/343 of query aligns to 2:344/347 of 5envA
- active site: C43 (= C43), H44 (= H44), T45 (= T45), H48 (= H48), H66 (= H66), E67 (= E67), C97 (= C97), C100 (= C100), C103 (= C103), C111 (= C111), D115 (≠ V115), C153 (= C153), R340 (= R336)
- binding trifluoroethanol: T45 (= T45), W54 (= W54), H66 (= H66), W92 (= W92), C153 (= C153), M270 (≠ L267), Y294 (≠ I290)
- binding nicotinamide-adenine-dinucleotide: H44 (= H44), T45 (= T45), H48 (= H48), T157 (= T157), G177 (= G177), G180 (= G179), G181 (= G180), L182 (= L181), D201 (= D200), K206 (= K205), F221 (≠ G220), S246 (≠ A243), V268 (≠ N265), G269 (= G266), V295 (= V291)
- binding zinc ion: C43 (= C43), H66 (= H66), C97 (= C97), C100 (= C100), C103 (= C103), C111 (= C111), C153 (= C153)
4w6zA Yeast alcohol dehydrogenase i, saccharomyces cerevisiae fermentative enzyme (see paper)
41% identity, 99% coverage: 2:340/343 of query aligns to 2:344/347 of 4w6zA
- active site: C43 (= C43), H44 (= H44), T45 (= T45), H48 (= H48), H66 (= H66), E67 (= E67), C97 (= C97), C100 (= C100), C103 (= C103), C111 (= C111), D115 (≠ V115), C153 (= C153), R340 (= R336)
- binding nicotinamide-8-iodo-adenine-dinucleotide: C43 (= C43), H44 (= H44), T45 (= T45), H48 (= H48), W54 (= W54), C153 (= C153), T157 (= T157), G177 (= G177), G180 (= G179), G181 (= G180), L182 (= L181), I200 (≠ V199), D201 (= D200), K206 (= K205), F221 (≠ G220), S246 (≠ A243), S248 (= S245), A251 (= A248), V268 (≠ N265), G269 (= G266), M270 (≠ L267), S293 (= S289), Y294 (≠ I290), V295 (= V291), R340 (= R336)
- binding trifluoroethanol: T45 (= T45), W54 (= W54), H66 (= H66), W92 (= W92), C153 (= C153)
- binding zinc ion: C43 (= C43), H66 (= H66), C97 (= C97), C100 (= C100), C103 (= C103), C111 (= C111), C153 (= C153)
P00330 Alcohol dehydrogenase 1; Alcohol dehydrogenase I; ADHI; NADH-dependent methylglyoxal reductase; YADH-1; EC 1.1.1.1; EC 1.1.1.54; EC 1.1.1.78 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
41% identity, 99% coverage: 2:340/343 of query aligns to 3:345/348 of P00330
- C44 (= C43) binding
- H45 (= H44) binding ; mutation to R: Decreases dissociation constants by 4-fold for NAD(+) and 2-fold for NADH, while turnover numbers were decreased by 4-fold for ethanol oxidation and 6-fold for acetaldehyde reduction.
- T46 (= T45) binding ; mutation to S: Has the same pattern of activity as the wild-type enzyme for linear primary alcohols.
- H49 (= H48) binding
- W55 (= W54) mutation to M: Has lowered reactivity with primary and secondary alcohols.
- H67 (= H66) binding
- E68 (= E67) binding in the open conformation
- W93 (= W92) mutation to A: Has an inverted specificity pattern for primary alcohols, being 3- and 10-fold more active on hexanol and 350- and 540-fold less active on ethanol. Also acquires weak activity on branched chain alcohols and cyclohexanol.
- C98 (= C97) binding
- C101 (= C100) binding
- C104 (= C103) binding
- C112 (= C111) binding
- C154 (= C153) binding
- G181 (= G179) binding
- G182 (= G180) binding
- L183 (= L181) binding
- D202 (= D200) binding
- K207 (= K205) binding
- F222 (≠ G220) binding
- T236 (≠ E232) natural variant: T -> I
- V269 (≠ N265) binding
- M271 (≠ L267) binding ; mutation to L: Produces a 7 to 10-fold increase in reactivity with butanol, pentanol, and hexanol.
- S294 (= S289) binding
- V296 (= V291) binding
- R341 (= R336) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylserine
Query Sequence
>Echvi_3219 FitnessBrowser__Cola:Echvi_3219
MLPKTMKAAVVTEFGQPLKIEEIPVKAPNENQILVQVMASGVCHTDLHAADGDWPVKPRL
PLIPGHEGIGYVAAVGSNVKNTKEGDIVGVPWLYSACGHCEHCITGWETLCESQVNGGYS
VDGGYAEYVLADPNYVGRFSGAIDFVQMAPILCAGVTVYKGLKETEVRPGQWVAISGIGG
LGHVAVQYAKAMGLHVLAVDVSDDKLNLAKKLGADRVVNGKNPDEVMNARKETGGVHGVL
VTAVSPVAFRQALDLLRRKGTLVMNGLPPGSFDLPIFETVLNRYTVRGSIVGTRKDLQEA
IDFAMEGKVTTTVKSAPLEDINLIFDQMKKGQIEGRMVLDIGH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory