SitesBLAST
Comparing GFF1129 FitnessBrowser__Marino:GFF1129 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
51% identity, 99% coverage: 2:337/338 of query aligns to 3:337/337 of 1krhA
- active site: C306 (= C306)
- binding flavin-adenine dinucleotide: Y143 (= Y140), R155 (= R152), S156 (≠ A153), Y157 (= Y154), S158 (= S155), V171 (≠ L170), V172 (≠ M171), G178 (= G177), K179 (≠ L178), M180 (= M179), S181 (= S180), T219 (= T218), E332 (= E332), F334 (= F334), S335 (≠ T335), A336 (= A336), N337 (= N337)
- binding fe2/s2 (inorganic) cluster: M38 (≠ L37), D39 (= D38), C40 (= C39), R41 (= R40), G43 (= G42), C45 (= C44), G46 (= G45), C48 (= C47), C82 (= C79)
1tvcA Fad and nadh binding domain of methane monooxygenase reductase from methylococcus capsulatus (bath) (see paper)
34% identity, 67% coverage: 108:334/338 of query aligns to 13:244/250 of 1tvcA
- active site: Y63 (= Y154), S64 (= S155), L215 (= L305)
- binding dihydroflavine-adenine dinucleotide: N24 (≠ T119), F49 (≠ Y140), R61 (= R152), S62 (≠ A153), Y63 (= Y154), S64 (= S155), L78 (= L170), I79 (≠ M171), R80 (= R172), L82 (≠ V174), F87 (≠ M179), G128 (= G219), N155 (≠ T246), E159 (≠ D250), S186 (≠ C278), G187 (= G279), E242 (= E332), F244 (= F334)
Sites not aligning to the query:
5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
28% identity, 95% coverage: 14:335/338 of query aligns to 9:330/333 of 5ogxA
- binding flavin-adenine dinucleotide: Y132 (= Y140), R144 (= R152), Q145 (≠ A153), Y146 (= Y154), S147 (= S155), H161 (≠ L170), V162 (≠ M171), V165 (= V174), G168 (= G177), V169 (≠ L178), A170 (≠ M179), T171 (≠ S180), T214 (= T218), F329 (= F334)
- binding fe2/s2 (inorganic) cluster: C34 (= C39), N35 (≠ R40), G37 (= G42), C39 (= C44), G40 (= G45), C42 (= C47), C74 (= C79)
Sites not aligning to the query:
P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
28% identity, 95% coverage: 14:335/338 of query aligns to 10:331/334 of P0DPQ8
- C35 (= C39) binding [2Fe-2S] cluster
- C40 (= C44) binding [2Fe-2S] cluster
- C43 (= C47) binding [2Fe-2S] cluster
- C75 (= C79) binding [2Fe-2S] cluster
- RQYS 145:148 (≠ RAYS 152:155) binding FAD
- HVR 162:164 (≠ LMR 170:172) binding FAD
- VAT 170:172 (≠ LMS 178:180) binding FAD
- T215 (= T218) binding FAD
- F330 (= F334) binding FAD
Sites not aligning to the query:
4wqmA Structure of the toluene 4-monooxygenase nadh oxidoreductase t4mof, k270s k271s variant (see paper)
29% identity, 96% coverage: 10:334/338 of query aligns to 7:325/326 of 4wqmA
- binding flavin-adenine dinucleotide: C36 (= C39), S38 (≠ D41), W57 (≠ I62), Y135 (= Y140), R146 (= R152), A147 (= A153), Y148 (= Y154), S149 (= S155), I162 (≠ L170), V163 (≠ M171), G169 (= G177), K170 (≠ L178), V171 (≠ M179), S172 (= S180), S209 (≠ T218), F325 (= F334)
- binding fe2/s2 (inorganic) cluster: Y34 (≠ L37), C36 (= C39), N37 (≠ R40), G39 (= G42), C41 (= C44), G42 (= G45), C44 (= C47), C76 (= C79)
Q03304 Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component; T4MO; Ferredoxin--NAD(+) reductase; Toluene-4-monooxygenase systme, electron transfer component; EC 1.18.1.3 from Ectopseudomonas mendocina (Pseudomonas mendocina) (see paper)
29% identity, 96% coverage: 10:334/338 of query aligns to 7:325/326 of Q03304
- C36 (= C39) binding [2Fe-2S] cluster
- C41 (= C44) binding [2Fe-2S] cluster
- C44 (= C47) binding [2Fe-2S] cluster
- C76 (= C79) binding [2Fe-2S] cluster
- RAYS 146:149 (= RAYS 152:155) binding FAD
- IVK 162:164 (≠ LMR 170:172) binding FAD
- KVS 170:172 (≠ LMS 178:180) binding FAD
7c3aA Ferredoxin reductase in carbazole 1,9a-dioxygenase (see paper)
29% identity, 91% coverage: 27:334/338 of query aligns to 27:320/321 of 7c3aA
- binding flavin-adenine dinucleotide: C39 (= C39), S41 (≠ D41), W60 (≠ G60), R152 (= R152), A153 (= A153), Y154 (= Y154), S155 (= S155), Y169 (≠ L170), I170 (≠ M171), G176 (= G177), R177 (≠ L178), F178 (≠ M179), S179 (= S180), A218 (≠ T218), F320 (= F334)
- binding fe2/s2 (inorganic) cluster: Y37 (≠ L37), C39 (= C39), A40 (≠ R40), G42 (= G42), C44 (= C44), G45 (= G45), C47 (= C47), C80 (= C79)
Sites not aligning to the query:
7c3bC Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
28% identity, 91% coverage: 27:334/338 of query aligns to 28:333/334 of 7c3bC
7romA Crystal structure of saccharomyces cerevisiae nadh-cytochrome b5 reductase 1 (cbr1) fragment (residues 28-284) bound to fad
33% identity, 51% coverage: 142:315/338 of query aligns to 51:230/255 of 7romA
- binding flavin-adenine dinucleotide: R61 (= R152), S62 (≠ A153), Y63 (= Y154), T64 (≠ S155), L79 (= L170), V80 (≠ M171), Y83 (≠ V174), T85 (≠ E176), G86 (= G177), N87 (≠ L178), V88 (≠ M179), S89 (= S180), T127 (= T218), A130 (= A221)
7c3bB Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
27% identity, 91% coverage: 27:334/338 of query aligns to 27:305/306 of 7c3bB
3ozwA The crystal structure of flavohemoglobin from r. Eutrophus in complex with ketoconazole (see paper)
27% identity, 67% coverage: 110:334/338 of query aligns to 159:396/403 of 3ozwA
- binding flavin-adenine dinucleotide: R206 (= R152), Q207 (≠ A153), Y208 (= Y154), S209 (= S155), S222 (= S168), V223 (≠ F169), K224 (≠ L170), E226 (≠ R172), P232 (= P175), G234 (= G177), Y235 (≠ L178), V236 (≠ M179), S237 (= S180), V276 (≠ T218), T279 (≠ A221), V395 (≠ K333), F396 (= F334)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 50
- binding protoporphyrin ix containing fe: 42, 43, 81, 85, 88, 90, 94, 95, 98, 126, 130, 133
- binding 1-acetyl-4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazine: 53, 56, 102, 398
3ozvA The crystal structure of flavohemoglobin from r. Eutrophus in complex with econazole (see paper)
27% identity, 67% coverage: 110:334/338 of query aligns to 159:396/403 of 3ozvA
- binding flavin-adenine dinucleotide: R206 (= R152), Q207 (≠ A153), Y208 (= Y154), S209 (= S155), S222 (= S168), V223 (≠ F169), K224 (≠ L170), E226 (≠ R172), P232 (= P175), G234 (= G177), Y235 (≠ L178), V236 (≠ M179), S237 (= S180), V276 (≠ T218), T279 (≠ A221), F396 (= F334)
Sites not aligning to the query:
- binding 1-[(2s)-2-[(4-chlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1h-imidazole: 24, 28, 57, 102, 106
- binding flavin-adenine dinucleotide: 44, 46, 48
- binding protoporphyrin ix containing fe: 43, 81, 84, 85, 88, 90, 94, 95, 98, 126, 130, 133
3ozuA The crystal structure of flavohemoglobin from r. Eutrophus in complex with miconazole (see paper)
27% identity, 67% coverage: 110:334/338 of query aligns to 159:396/403 of 3ozuA
- binding flavin-adenine dinucleotide: R206 (= R152), Q207 (≠ A153), Y208 (= Y154), S209 (= S155), S222 (= S168), V223 (≠ F169), K224 (≠ L170), E226 (≠ R172), P232 (= P175), G234 (= G177), Y235 (≠ L178), V236 (≠ M179), S237 (= S180), E394 (= E332), V395 (≠ K333)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 47, 397, 398
- binding protoporphyrin ix containing fe: 43, 44, 81, 85, 88, 90, 94, 95, 98, 126, 129, 130, 133
- binding 1-[(2R)-2-[(2,4-dichlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1H-imidazole: 25, 28, 43, 56, 57, 102, 122, 125, 126
P39662 Flavohemoprotein; FHP; Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (Ralstonia eutropha) (see paper)
27% identity, 67% coverage: 110:334/338 of query aligns to 159:396/403 of P39662
Sites not aligning to the query:
- 60 A→Y: Does not affect phospholipid-binding.
- 98 V→F: Blocks phospholipid-binding.
1cqxA Crystal structure of the flavohemoglobin from alcaligenes eutrophus at 1.75 a resolution (see paper)
28% identity, 67% coverage: 110:334/338 of query aligns to 159:396/403 of 1cqxA
- binding flavin-adenine dinucleotide: Y190 (= Y140), R206 (= R152), Q207 (≠ A153), Y208 (= Y154), S209 (= S155), S222 (= S168), E226 (≠ R172), Q231 (≠ V174), P232 (= P175), G234 (= G177), Y235 (≠ L178), V236 (≠ M179), S237 (= S180), V395 (≠ K333)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 47, 397, 398
- binding protoporphyrin ix containing fe: 43, 44, 81, 85, 88, 90, 94, 95, 98, 126, 129, 130, 133
6l2uA Soluble methane monooxygenase reductase fad-binding domain from methylosinus sporium. (see paper)
34% identity, 29% coverage: 104:202/338 of query aligns to 2:103/103 of 6l2uA
- binding flavin-adenine dinucleotide: F43 (≠ Y140), R55 (= R152), S56 (≠ A153), Y57 (= Y154), S58 (= S155), F71 (≠ L170), F71 (≠ L170), I72 (≠ M171), R73 (= R172), L75 (≠ V174), P76 (= P175), P76 (= P175), G78 (= G177), A79 (≠ L178), F80 (≠ M179), S81 (= S180)
2eixA The structure of physarum polycephalum cytochrome b5 reductase (see paper)
26% identity, 57% coverage: 138:330/338 of query aligns to 45:241/243 of 2eixA
- active site: H47 (≠ Y140), Y63 (= Y154), T64 (≠ S155), C217 (= C306)
- binding flavin-adenine dinucleotide: R61 (= R152), P62 (≠ A153), Y63 (= Y154), T64 (≠ S155), I78 (≠ L170), Y82 (≠ V174), K84 (≠ E176), G85 (= G177), M87 (= M179), S88 (= S180), T126 (= T218), T129 (≠ A221), F195 (≠ Y286), M221 (≠ P310), A225 (= A314)
1rfkB Crystal structure of 2fe2s ferredoxin from thermophilic cyanobacterium mastigocladus laminosus (see paper)
37% identity, 28% coverage: 2:97/338 of query aligns to 3:97/97 of 1rfkB
7qu0A X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
25% identity, 47% coverage: 177:334/338 of query aligns to 116:279/279 of 7qu0A
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 40, 83, 84, 85, 86, 104, 105, 106, 107, 113, 114, 115
7qtyA X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
25% identity, 47% coverage: 177:334/338 of query aligns to 116:279/279 of 7qtyA
- binding 1-(furan-2-ylmethyl)-3-(2-methylphenyl)thiourea: A184 (≠ V245), R185 (≠ T246), S213 (≠ A274), F227 (≠ Y286), H229 (≠ T288), V255 (≠ P310), M256 (= M311)
- binding flavin-adenine dinucleotide: G116 (= G177), I117 (≠ L178), M118 (= M179), S119 (= S180), F279 (= F334)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 40, 83, 84, 85, 86, 100, 104, 105, 107, 113, 114
Query Sequence
>GFF1129 FitnessBrowser__Marino:GFF1129
MYKVALNFEDGITRFITTSPMETVANAAYRSGINIPLDCRDGACGTCKCHCESGTYEMEG
FIEDALSDEEVRLGYVLTCQMIPESDCVVTVPASSEVCLKGGASDIFAELSEVKQLSRTA
LSFVVSGEGVRAMQFLPGQYAKIQVPGTDEFRAYSFSSMVNHANGLVSFLMRVVPEGLMS
GYMTHKSSVGDSIALRGPFGSFYLREVKRPVLMLAGGTGLAPFLAMLEKIQLEGSDYPIH
MVYGVTHEVDLVALDQLEAFAEAIGNFTFSACVASSECGSWPQKGYVTHHIEPEHLNDGN
VDIYLCGPPPMVDAVSSFIQEKGIQPVSFYYEKFTANK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory