SitesBLAST

T49 (= T46) mutation T->F,W: Increases uptake of both methylarsenite and methylarsenate and exhibits higher sensitivity to methylarsenite.
V177 (= V175) mutation to I: Decreases methylarsenite transport activity and results in higher resistance to methylarsenite. Slight decrease in methylarsenate transport activity.; mutation to R: Increases methylarsenite transport activity, leading to lower resistance to methylarsenite. Nearly complete loss of methylarsenate transport activity.

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
39% identity, 63% coverage: 4:141/218 of query aligns to 38:173/271 of P08995

query
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P08995

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Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

Q9SAI4 Aquaporin NIP6-1; NOD26-like intrinsic protein 6-1; AtNIP6;1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 62% coverage: 4:139/218 of query aligns to 80:212/305 of Q9SAI4

query
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Q9SAI4

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A119 (≠ T46) mutation to W: 6-fold increase in water transport activity, but impaired in urea transport.

Sites not aligning to the query:

252 V→A: No effect.

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
31% identity, 85% coverage: 4:189/218 of query aligns to 49:237/298 of Q6Z2T3

query
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Q6Z2T3

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A132 (= A90) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

O94778 Aquaporin-8; AQP-8 from Homo sapiens (Human) (see 4 papers)
43% identity, 44% coverage: 4:98/218 of query aligns to 36:124/261 of O94778

query
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O94778

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C38 (≠ L6) mutation to S: Does not affect loss of hydrogen peroxide transport after stress.
F48 (= F15) mutation to A: Loss of hydrogen peroxide transport activity under stress condition.
C53 (≠ V20) modified: Cysteine persulfide; modified: Cysteine sulfenic acid (-SOH); mutation to S: Does not affect hydrogen peroxide transport under stress condition.
H72 (≠ T46) mutation to A: Does not affect hydrogen peroxide transport activity under stress condition.

Sites not aligning to the query:

8 C→S: Does not affect loss of hydrogen peroxide transport after stress.
173 C→A: Loss of hydrogen peroxide transporter activity.; C→S: Slightly affect hydrogen peroxide transport after stress.
208 C→S: Does not affect loss of hydrogen peroxide transport after stress.
213 R→A: Does not affect hydrogen peroxide transport activity under stress condition.
229 I → M: in a breast cancer sample; somatic mutation
247 C→S: Does not affect loss of hydrogen peroxide transport after stress.

3nkaA Crystal structure of aqpz h174g,t183f (see paper)
37% identity, 43% coverage: 4:97/218 of query aligns to 5:96/230 of 3nkaA

query
sites
3nkaA

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binding glycerol: A25 (≠ G24), V41 (≠ N42)

Sites not aligning to the query:

binding glycerol: 180, 184

P60844 Aquaporin Z; Bacterial nodulin-like intrinsic protein; Water channel AqpZ from Escherichia coli (strain K12) (see paper)
37% identity, 43% coverage: 4:97/218 of query aligns to 3:94/231 of P60844

query
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P60844

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C9 (≠ A10) mutation to S: No effect.
C20 (≠ V21) mutation to S: Loss of oligomerization; no alteration of water permeability.

Sites not aligning to the query:

183 T→C: No effect.
189 mutation R->V,S: Loss of function.

2o9eA Crystal structure of aqpz mutant t183c complexed with mercury (see paper)
37% identity, 43% coverage: 4:97/218 of query aligns to 5:96/232 of 2o9eA

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2o9eA

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Sites not aligning to the query:

binding mercury (ii) ion: 140, 179

P30302 Aquaporin PIP2-3; Plasma membrane intrinsic protein 2-3; AtPIP2;3; Plasma membrane intrinsic protein 2c; PIP2c; RD28-PIP; TMP2C; Water stress-induced tonoplast intrinsic protein; WSI-TIP from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 69% coverage: 4:153/218 of query aligns to 37:201/285 of P30302

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P30302

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Sites not aligning to the query:

223 T→C: Creates some mercury-sensitivity.

I1CR68 Aquaporin-1 from Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar) (see paper)
27% identity, 62% coverage: 4:138/218 of query aligns to 59:191/306 of I1CR68

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I1CR68

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E

Q

K

W

S

F

T

S

A

E

G

I

I

L

F

A

G

T

T

F

Y

G

P

L

P

L

L

F

Y

V

V

Sites not aligning to the query:

275 H→A: Affects pH sensing; when associated with A-85.

P43287 Aquaporin PIP2-2; Plasma membrane intrinsic protein 2-2; AtPIP2;2; Plasma membrane intrinsic protein 2b; PIP2b; TMP2b from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 69% coverage: 4:153/218 of query aligns to 37:201/285 of P43287

query
sites
P43287

Q

R

K

A

L

V

I

I

A

A

E

E

A

F

L

V

G

A

T

T

G

L

F

L

L

F

L

L

-

Y

-

I

-

T

-

V

I

L

G

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V

I

G

G

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Y

G

K

I

I

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A

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G

N

V

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D

-

C

-

G

G

G

L

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A

G

L

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L

L

A

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N

I

A

A

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W

A

A

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F

G

G

A

G

M

M

L

I

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F

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I

I

L

I

V

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Y

T

C

L

T

G

A

P

G

I

I

S

S

G

G

A

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

A

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F

L

A

F

L

L

R

A

G

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D

K

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L

A

I

A

R

V

A

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P

L

Y

Y

V

M

A

V

A

A

Q

Q

I

C

I

L

G

G

G

A

I

I

L

C

G

G

V

V

W

G

A

F

S

-

H

-

V

-

M

-

F

-

D

-

L

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T

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I

A

L

F

Q

Q

T

S

S

S

T

Y

M

D

H

R

R

Y

T

G

G

G

A

A

Q

N

W

S

F

L

-

A

-

D

-

G

-

Y

-

N

-

T

-

G

-

T

-

G

-

L

-

A

S

A

E

E

I

I

L

I

A

G

T

T

F

F

G

V

L

L

L

V

F

Y

V

T

I

V

F

F

G

S

G

A

-

T

-

D

-

P

-

K

L

R

R

N

S

A

R
|
R

P
x
D

E

S

A
x
H

V

V

P

P

A

V

L

L

R194 (= R146) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
D195 (≠ P147) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
H197 (≠ A149) mutation to A: Reduced water transport activity. Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-194 or A-195.; mutation to D: Reduced water transport activity. Insensitive to cytosolic acidification.; mutation to K: Very low water transport activity. Insensitive to cytosolic acidification.

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial
264 H→A: No effect.

P43286 Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 69% coverage: 4:153/218 of query aligns to 39:203/287 of P43286

query
sites
P43286

Q

R

K

A

L

V

I

I

A

A

E

E

A

F

L

V

G

A

T

T

G

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F

L

L

F

L

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-

Y

-

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-

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-

V

I

L

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M

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T

C

L

T

G

A

P

G

I

I

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S

G

G

A

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

A

G

F

L

A

F

L

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R

A

G

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D

K

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L

A

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A

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L

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Y

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I

A

I

A

A

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Q

I

C

I

L

G

G

G

A

I

I

L

C

G

G

V

V

W

G

A

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S

-

H

-

V

-

M

-

F

-

D

-

L

V

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K

I

A

L

F

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Q

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S

S

T

Y

M

T

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R

R

Y

T

G

G

G

A

A

Q

N

W

S

F

L

-

A

-

D

-

G

-

Y

-

S

-

T

-

G

-

T

-

G

-

L

-

A

S

A

E

E

I

I

L

I

A

G

T

T

F

F

G

V

L

L

L

V

F

Y

V

T

I

V

F

F

G

S

G

A

L

T

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D

-

P

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K

-

R

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S

A

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D

E

S

A

H

V

V

P

P

A

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L

L

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial; K→A: 2-fold decrease in water transport activity.; K→R: No effect.
6 E→A: No effect.
280 modified: Phosphoserine; S→A: Normal subcellular localization.
283 modified: Phosphoserine; S→A: Intracellular reticulation pattern, probably corresponding to the endoplasmic reticulum.; S→D: Normal subcellular localization.

P55064 Aquaporin-5; AQP-5 from Homo sapiens (Human) (see 2 papers)
31% identity, 65% coverage: 39:179/218 of query aligns to 42:198/265 of P55064

query
sites
P55064

L

I

L

L

A

Q

N
x
I

A

A

V

L

A

A

T

F

G

G

A

L

M

A

L

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Y

G

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T

A

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L

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G

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P

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S

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G

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G

H

H

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I

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N

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L

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L

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G

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S

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L

A

L

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F

Y

Y

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A

A

Q

Q

I

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I

V

G

G

G

A

I

I

L

A

G

G

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-

W

-

A

-

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A

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G

V

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M

L

F

Y

D

G

L

V

T

A

I

P

L

L

Q

N

T

A

S

R

-

G

-

N

-

L

-

A

-

V

-

N

-

A

T

L

T
x
N

M

N

H

N

R

T

T

T

G

Q

G

G

A

Q

Q

A

W

M

F

V

S

V

E

E

I

L

L

I

A

L

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T

F

F

G

Q

L

L

L

A

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L

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C

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F

G

A

G

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L

T

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D

S

S

R

R

-

R

-

T

-
x
S

P

P

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V

A

G

V

S

P

P

A

A

-

L

-

S

-

I

-

G

-

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S

-

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-

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-

L

-

G

-

H

L

L

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V

A

G

F
x
I

Y

Y

I

F

T

T

G

G

A

C

Y

S

W

-

F

M

T

N

A

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S

A

T
x
R

S

S

F

F

A

-

N

G

P

P

A

A

V

V

T

V

V

M

A

N

R

R

I45 (≠ N42) to S: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123055
N123 (≠ T116) to D: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123057
S156 (vs. gap) mutation to A: No effect on location at the cell membrane.; mutation to E: Increased location at the cell membrane.
I177 (≠ F156) to F: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123056
R188 (≠ T168) to C: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs368292687

Sites not aligning to the query:

38 A → E: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123054

5dyeD Crystal structure of the full length s156e mutant of human aquaporin 5 (see paper)
31% identity, 65% coverage: 39:179/218 of query aligns to 41:197/253 of 5dyeD

query
sites
5dyeD

L

I

L

L

A

Q

N

I

A

A

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G

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L

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-

G

-

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-

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-

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-

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-

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G

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Q

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C

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T

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D

S

S

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R

-

R

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T

-

E

P

P

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A
x
G

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x
S

P

P

A

A

-

L

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-

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L

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L

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Y

I

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G

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W

-

F

M

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S

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F

A

-

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G

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M

A

N

R

R

binding O-[(S)-{[(2S)-2-(hexanoyloxy)-3-(tetradecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-D-serine: G158 (≠ A149), S159 (≠ V150)

P47863 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Rattus norvegicus (Rat) (see 4 papers)
32% identity, 51% coverage: 47:158/218 of query aligns to 78:193/323 of P47863

query
sites
P47863

G

G

A

L

M

S

L

I

Y

A

V

T

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M

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T

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L

F

G

G

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S

G

G

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G

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H

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N

N

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P

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A

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A

A

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Q

I

C

I

L

G

G

G

A

I

I

L

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G

G

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A

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G

A

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Y

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L

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S

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V

L

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Q

G

-

G

-

L

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G

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T

M

V

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H

-

G

-

N

R

L

T

T

G

A

G

G

A

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G

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L

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V

E

E

I

L

L

I

A

I

T

T

F

F

G

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L

L

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G

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|
S

R

K

P

R

E

T

A

D

V

V

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G

L

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V

A

A

F

L

Y

A

I

I

S180 (= S145) modified: Phosphoserine; by PKC; mutation to A: Decreases internalization from the cell membrane in response to PKC activation.

Sites not aligning to the query:

13 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-17.
17 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-13.
201 H→P: Partial loss of transport activity.
285 modified: Phosphoserine

P09011 Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26 from Rattus norvegicus (Rat) (see 2 papers)
44% identity, 28% coverage: 37:98/218 of query aligns to 37:98/261 of P09011

query
sites
P09011

L

L

A

H

L

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L

L

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A

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A

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Q

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L

G

G

G

A

I

V

L

A

G

G

Sites not aligning to the query:

233 modified: Phosphoserine
244 N→D: No effects.

P55088 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Mus musculus (Mouse) (see 2 papers)
31% identity, 51% coverage: 47:158/218 of query aligns to 78:193/323 of P55088

query
sites
P55088

G

G

A

L

M

S

L

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A

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G

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G

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N

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S

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I

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G

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L

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A

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L

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A

I

I

S111 (= S80) modified: Phosphoserine; by PKG; mutation to A: Loss of phosphorylation by PKG (in vitro). No effect on location at cell membrane.

Sites not aligning to the query:

4 natural variant: G -> R

P23645 Neurogenic protein big brain from Drosophila melanogaster (Fruit fly) (see 3 papers)
33% identity, 46% coverage: 39:139/218 of query aligns to 99:196/696 of P23645

query
sites
P23645

L

L

A

A

N

T

A

A

V

L

A

A

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G

G

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M

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G

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A

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N

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L

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D

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-

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-

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-

G

-

N

L

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Q

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S

A

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I

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S

M

-

H

H

R

S

T

A

G

A

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L

A

A

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A

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W

F

-

S

-

E

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I

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L

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A

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F

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G

G

L

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L

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I

L

Sites not aligning to the query:

46 modified: Phosphoserine
47 modified: Phosphothreonine
71 E→D: Partial loss of transport activity and increased sensitivity to blocking by the magnesium ion.; E→K: Loss of expression in cell membrane.; E→N: Loss of transport activity.; E→Q: Loss of transport activity.
253 D→N: No effect on transport activity.
274 E→Q: No effect on transport activity.
300 modified: Phosphoserine
394 modified: Phosphoserine
576 modified: Phosphoserine

6f7hC Crystal structure of human aqp10 (see paper)
27% identity, 60% coverage: 4:133/218 of query aligns to 7:134/253 of 6f7hC

query
sites
6f7hC

Q

R

K

Q

L

C

I

L

A

A

E

E

A

F

L

L

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G

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G

F

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L

L

M

I

L

G

L

V

T

V

Q

G

G

S

A

G

V

I

A

M

Q

A

A

E

V

T

T

-

S

-

G

L

E

S

T

G

K

G

G

N

N

I

F

G

F

L

T

A

M

L

F

L

L

A

A

N

G

A

S

V

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A

A

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A

I

M

A

L
x
I

Y

Y

V

V

I

G

I

-

T

-

L

-

G

G

P

N

I

V

S

S

G

G

A

A

H

H

F

L

N

N

P

P

A

A

V

F

T

S

L

L

A

A

F

M

A

C

L

I

R

V

G

G

D

R

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L

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P

W

W

A

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A

K

V

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P

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Y

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A

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A

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Q

I

L

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S

G

A

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F

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A

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G

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A

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H

Y

V

V

M

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F

Y

D

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L

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I

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L

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Q

N

T

Y

S

T

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T

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G

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T

T

F

Y

binding glycerol: I55 (≠ L50)

Sites not aligning to the query:

binding glycerol: 199

P37451 Propanediol uptake facilitator PduF from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
33% identity, 53% coverage: 8:123/218 of query aligns to 11:123/264 of P37451

query
sites
P37451

A

A

E

E

A

F

L

L

G

G

T

T

G

G

F

L

L

F

L

L

I

F

G

F

V

G

V

I

G

G

S

C

G

-

I

L

M

S

A

A

E

L

T

K

L

V

S

A

G

G

G

A

N

S

I

L

G

G

L

L

A

W

L

E

L

I

A

C

N

I

A

I

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W

A

G

T

L

G

G

A

I

M

S

L

L

Y

A

V

V

I

Y

I

L

T

T

T

A

L

-

G

-

P

G

I

I

S

S

G

G

A

G

H

H

F

L

N
|
N

P
|
P

A
|
A

V

V

T

T

L

I

A

A

F

L

A

W

L

L

R

F

G

A

D

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P

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A

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A

K

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V

P

L

P

P

Y

Y

V

I

A

I

A

A

Q

Q

I

F

I

A

G

G

G

A

I

F

L

G

G

G

V

A

W

L

A

L

S

A

H

Y

V

V

M

L

F

Y

D

S

L

S

T

L

I

F

L

T

Q

E

T

F

S

E

T

T

T

A

M

H

H

H

R

M

T

V

G

R

G

G

A

S

NPA 66:68 (= NPA 66:68) NPA 1

Sites not aligning to the query:

201:203 NPA 2

Query Sequence

>GFF1479 FitnessBrowser__Phaeo:GFF1479
MTTQKLIAEALGTGFLLIGVVGSGIMAETLSGGNIGLALLANAVATGAMLYVIITTLGPI
SGAHFNPAVTLAFALRGDHSWAAVPPYVAAQIIGGILGVWASHVMFDLTILQTSTTMHRT
GGAQWFSEILATFGLLFVIFGGLRSRPEAVPALVAFYITGAYWFTASTSFANPAVTVARG
FSDTFAGIYPGHILMFIVMQFIGVGLAHLILPRLFRPS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory