SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2493 FitnessBrowser__WCS417:GFF2493 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ryaA Crystal structure of abc transporter solute binding protein avi_3567 from agrobacterium vitis s4, target efi-510645, with bound d-mannitol
65% identity, 94% coverage: 27:440/440 of query aligns to 1:417/417 of 4ryaA

query
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4ryaA

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binding d-mannitol: E40 (= E66), R44 (= R70), Y114 (= Y138), E116 (= E140), R163 (= R187), G169 (= G193), F224 (= F248), W279 (≠ Y303), W281 (= W305), Q367 (= Q390)

5iaiA Crystal structure of abc transporter solute binding protein arad_9887 from agrobacterium radiobacter k84, target efi-510945 in complex with ribitol
27% identity, 92% coverage: 29:431/440 of query aligns to 7:397/399 of 5iaiA

query
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5iaiA

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binding D-ribitol: D15 (≠ N37), D67 (≠ G90), Q176 (≠ M196), W227 (≠ F248), W245 (≠ A266), G283 (≠ S304), W284 (= W305), R357 (≠ L391)

1eu8A Structure of trehalose maltose binding protein from thermococcus litoralis (see paper)
27% identity, 88% coverage: 50:435/440 of query aligns to 27:407/407 of 1eu8A

query
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1eu8A

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binding alpha-D-glucopyranose: T43 (≠ E66), T45 (≠ V68), R48 (≠ Q71), D69 (≠ G90), Y120 (= Y138), D122 (≠ E140), G178 (= G193), E238 (≠ N249), W256 (≠ A266), Y258 (≠ V268), G292 (≠ Y303), G293 (≠ S304), W294 (= W305), R363 (≠ K382)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 12, 16

Q7LYW7 Trehalose/maltose-binding protein MalE; TMBP from Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) (see paper)
26% identity, 98% coverage: 7:435/440 of query aligns to 19:448/450 of Q7LYW7

query
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Q7LYW7

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F

T

G

P

A

T

Q

E

T

T

-

S

-

L

-

Q

-

G

-

K

L

I

T

V

I

F

A

A

T

V

V

G

N

G

N

A

S

P

D

N

M
x
E

I

I

R

E

M

Y

Q

W

K

K

-

G

L

V

S

I

K

A

T

E

F

F

E

E

A

K

E

K

H

Y

P

P

E

G

I

V

K

T

L

V

N

E

W

L

V

K

V

R

L

Q

E

A

E
x
T

N

D

V

T

L

E

R

Q

Q
x
R

R

R

L

L

T

D

T

L

D

V

I

N

A

A

T

L

Q

R

G

G

-

K

-

S

G

S

Q

D

F

P

D

D

V

V

L

F

T

L

I

M

G
x
D

M

V

Y

A

E

W

A

L

A

G

L

Q

W

F

G

I

A

A

K

S

G

G

W

W

L

L

E

E

P

P

M

L

K

D

D

D

L

Y

-

V

-

Q

P

K

A

D

S

N

Y

Y

D

D

L

L

D

S

D

V

V

F

F

F

P

Q

S

S

V

V

R

I

D

N

G

-

L

L

S

A

V

D

K

K

-

Q

-

G

G

G

S

K

L

L

Y

Y

A

A

L

L

P

P

F

V

Y
|
Y

A

I

E
x
D

S

A

S

G

I

L

T

L

Y

Y

R

R

T

K

D

D

L

L

F

L

K

E

D

K

A

Y

G

G

L

Y

T

S

M

K

P

P

E

P

H

E

P

-

T

T

W

W

T

Q

Q

E

I

L

G

V

E

E

F

M

A

A

S

Q

K

K

L

I

T

Q

D

S

K

G

T

E

K

R

E

E

Q

T

-

N

-

P

-

N

-

F

Y

W

G

G

L

F

C

V

L

W

R

Q

G

G

K

K

A

Q

G
x
Y

W

E

G

S

E

L

N

V

M

C

A

D

L

F

I

V

T

E

T

Y

L

V

A

Y

N

S

G

N

Y

G

G

G

A

S

-

L

-

G

R

E

W

F

F

K

D

D

E

G

K

K

W

W

Q

V

P

P

E

T

F

L

N

N

G

K

P

P

E

E

W

N

K

V

D

E

A

A

L

L

N

Q

F

F

Y

M

V

V

D

D

N

L

M

I

K

H

K

K

S

Y

-

K

-

I

G

S

P

P

G

N

A

T

S

Y

S

T

N

E

G

M

F

T

N
x
E

E

E

N

P

L

V

A

R

L

L

-

M

F

F

N

Q

S

Q

G

G

K

N

C

A

A

A

I

F

W

E

V

R

D

N

A
x
W

S

P

V
x
Y

A

A

G

W

S

G

F

-

V

L

T

H

D

N

K

A

T

D

Q

D

S

S

K

P

V

V

A

K

D

G

H

K

V

V

G

G

F

V

T

A

F

P

A

L

P

P

H

H

E

F

K

P

T

G

D

H

K

K

G

S

T

A

S

A

W

T

L

L

Y

G

S
x
G

W
|
W

S

H

L

I

A

G

I

I

P

S

T

K

S

Y

S

S

K

D

A

N

K

K

D

A

A

L

A

A

K

W

V

E

F

F

T

V

S

K

W

F

A

V

T

E

S

S

K

Y

E

S

Y

V

G

Q

E

K

L

G

V

F

A

A

K

M

T

N

D

L

G

G

I
x
W

A

N

N

-

V

-

P

-

P

P

G

G

T

-

R

R

K

V

S

D

T

V

Y

Y

N

D

D

D

-

P

E

A

Y

V

M

V

K

S

A

K

A

S

P

P

F

H

A

L

K

K

V

E

T

L

L

R

E

A

S

V

L

F

K

E

V

N

A

A

D

V

P

P

T

-

K

-

P

-

T

-

L

-

K
x
R

P

P

V

I

P

-

Y

-

I

-

G

-

I

-

Q

-

L

-

V

-

T

-

I

V

P

P

E

Y

F

Y

Q

P

A

Q

I

L

G

S

T

E

Q

I

V

I

G

Q

K

K

F

Y

F

V

S

N

G

S

A

A

L

L

T

A

G

G

Q

K

Q

I

T

S

V

P

D

Q

A

E

A

A

L

L

T

D

A

K

A

A

Q

Q

T

K

T

E

T

A

E

E

R

E

E

L

M

V

K

K

R

Q

E57 (≠ M40) binding alpha,alpha-trehalose
T84 (≠ E66) binding alpha,alpha-trehalose
R89 (≠ Q71) binding alpha,alpha-trehalose
D110 (≠ G90) binding alpha,alpha-trehalose
Y161 (= Y138) binding alpha,alpha-trehalose
D163 (≠ E140) binding alpha,alpha-trehalose
Y217 (≠ G191) binding alpha,alpha-trehalose
E279 (≠ N249) binding alpha,alpha-trehalose
W297 (≠ A266) binding alpha,alpha-trehalose
Y299 (≠ V268) binding alpha,alpha-trehalose
G334 (≠ S304) binding alpha,alpha-trehalose
W335 (= W305) binding alpha,alpha-trehalose
W371 (≠ I341) binding alpha,alpha-trehalose
R404 (≠ K382) binding alpha,alpha-trehalose

A9CEY9 Sulfoquinovosyl glycerol-binding protein SmoF; SQGro-binding protein SmoF; SQ monooxygenase cluster protein F from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see 2 papers)
27% identity, 75% coverage: 6:336/440 of query aligns to 9:335/416 of A9CEY9

query
sites
A9CEY9

K

K

A

A

L

L

L

M

A

G

S

A

T

A

C

L

M

C

M

A

T

T

C

M

M

L

T

T

L

F

S

S

A

G

V

Q

S

A

F

F

G

A

A

D

Q

A

T

E

L

L

T

K

I

I

A

F

T

V

V

S

N

S

N
x
Q

S
x
H

D

Q

M

P

I

D

R

I

M

W

Q

R

K

K

L

A

S

L

K

D

T

Q

F

Y

E

E

A

A

E

K

H

T

P

P

E

G

I

V

K

K

L

-

N

-

W

-

V

V

L

I

E

E

-

T

-

G

-

N

-

T

E
x
S

N

E

V

M

L

Q

R

A

Q

Q

R

Y

L

L

T

N

T

T

D

V

I

M

A

S

T

A

Q

K

G

D

G

S

Q

S

F

L

D

D

V

V

L

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

A

P

A

A

L

Q

W

F

G

A

A

T

K

A

G

G

W

W

L

T

E

S

P

D

M

F

K

-

D

-

L

-

P

-

A

S

S

G

Y

K

D

D

L

L

D

S

D

A

V

Y

F

L

P

P

S

T

V

Y

R

A

D

E

G

A

L

N

S

T

V

V

K

N

G

G

S

K

L

I

Y

V

A

A

L

L

P

P

F

A

Y

F

A

A

E
x
D

S

S

S

M

I

F

T

L

Y

Y

R

R

T

K

D

D

L

L

F

L

K

D

D

K

A

Y

G

G

L

I

T

K

M

P

P

P

E

-

H

-

P

T

T

T

W

W

T

D

Q

E

I

L

G

K

E

E

F

A

A

S

S

K

K

K

L

V

T

M

-

E

-

G

D

E

K

K

T

N

K

P

E

E

Q

L

Y

Q

G

G

L

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W

E

G
|
G

E

-

N

-

M

-

A

A

L

V

I

C

T

T

T

F

L

L

A

L

N

P

G

Y

Y

W

G

S

-

E

A

G

R

K

W

S

F

L

D

V

E

E

K

N

W

G

Q

K

P

L

E

N

F

F

N

D

G

N

P

K

E

A

W

A

K

V

D

D

A

S

L

L

N

K

F

L

-

W

-

K

-

S

Y

F

V

V

D

D

N

D

-

G

-

I

M

S

K

K

S

N

G

I

P

S

P

E

G

V

A

A

S
x
T

S

D

N

D

G

T

F

R

N

K

E

E

N

-

L

-

A

-

L

-

F

F

N

Q

S

A

G

G

K

K

C

V

A

L

I

F

W

A

V

V

D

N

A

W

S

S

V

Y

A

A

G

W

S

T

F

H

V

F

T

Q

D

G

K

K

T

-

Q

E

S

S

K

Q

V

V

A

N

D

D

H

K

V

V

G

G

F

V

T

A

F

R

A

L

P

P

H

A

E

V

K

K

T

G

D

G

K

E

G

Q

T

T

S

T

W

C

L

L

Y

G

S
x
G

W
|
W

S

E

L

F

A

G

I

V

P

S

T

A

S

Y

S

S

K

K

A

Q

K

Q

D

D

A

E

A

A

K

K

V

K

F

L

T

V

S

E

W

Y

A

L

T

S

S

S

K

Q

E

D

Y

V

G

S

E

K

L

F

V

M

A

A

Q40 (≠ N37) binding 3-(6-sulfo-alpha-D-quinovosyl)glycerol
H41 (≠ S38) binding 6-sulfo-D-quinovose
S71 (≠ E66) binding 3-(6-sulfo-alpha-D-quinovosyl)glycerol
D95 (≠ G90) binding 3-(6-sulfo-alpha-D-quinovosyl)glycerol
D141 (≠ E140) binding 3-(6-sulfo-alpha-D-quinovosyl)glycerol
G194 (= G193) binding 3-(6-sulfo-alpha-D-quinovosyl)glycerol
T248 (≠ S244) binding 3-(6-sulfo-alpha-D-quinovosyl)glycerol
G303 (≠ S304) binding 3-(6-sulfo-alpha-D-quinovosyl)glycerol; binding 6-sulfo-D-quinovose
W304 (= W305) binding 3-(6-sulfo-alpha-D-quinovosyl)glycerol; binding 6-sulfo-D-quinovose

Sites not aligning to the query:

373 binding 3-(6-sulfo-alpha-D-quinovosyl)glycerol; binding 6-sulfo-D-quinovose

7qhvAAA Sulfoquinovosyl binding protein (see paper)
28% identity, 65% coverage: 50:336/440 of query aligns to 24:305/390 of 7qhvAAA

query
sites
7qhvAAA

F

Y

E

E

A

A

E

K

H

T

P

P

E

G

I

V

K

K

L

-

N

-

W

-

V

V

L

I

E

E

-

T

-

G

-
x
N

-
x
T

E
x
S

N

E

V

M

L

Q

R

A

Q

Q

R

Y

L

L

T

N

T

T

D

V

I

M

A

S

T

A

Q

K

G

D

G

S

Q

S

F

L

D

D

V

V

L

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

A

P

A

A

L

Q

W

F

G

A

A

T

K

A

G

G

W

W

L

T

E

S

P

D

M

F

K

-

D

-

L

-

P

-

A

-

S

S

Y

K

D

D

L

L

D

S

D

A

V

Y

F

L

P

P

S

T

V

Y

R

A

D

E

G

A

L

N

S

T

V

V

K

N

G

G

S

K

L

I

Y

V

A

A

L

L

P

P

F

A

Y
x
F

A

A

E
x
D

S

S

S

M

I

F

T

L

Y

Y

R

R

T

K

D

D

L

L

F

L

K

D

D

K

A

Y

G

G

L

I

T

K

M

P

P

P

E

-

H

-

P

T

T

T

W

W

T

D

Q

E

I

L

G

K

E

E

F

A

A

S

S

K

K

K

L

V

T

M

-

E

-

G

D

E

K

K

T

N

K

P

E

E

Q

L

Y

Q

G

G

L

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W
x
E

G
|
G

E

-

N

-

M

-

A

A

L

V

I

C

T

T

T

F

L

L

A

L

N

P

G

Y

Y

W

G

S

-

E

A

G

R

K

W

S

F

L

D

V

E

E

K

N

W

G

Q

K

P

L

E

N

F

F

N

D

G

N

P

K

E

A

W

A

K

V

D

D

A

S

L

L

N

K

F

L

-

W

-

K

-

S

Y

F

V

V

D

D

N

D

-

G

-

I

M

S

K

K

S

N

G

I

P

S

P

E

G

V

A
|
A

S
x
T

S
x
D

N

D

G

T

F

R

N

K

E

E

N

-

L

-

A

-

L

-

F

F

N

Q

S

A

G

G

K

K

C

V

A

L

I

F

W

A

V

V

D

N

A
x
W

S

S

V

Y

A

A

G

W

S

T

F

H

V

F

T

Q

D

G

K

K

T

-

Q

E

S

S

K

Q

V

V

A

N

D

D

H

K

V

V

G

G

F

V

T

A

F

R

A

L

P

P

H

A

E

V

K

K

T

G

D

G

K

E

G

Q

T

T

S

T

W

C

L

L

Y
x
G

S
x
G

W
|
W

S

E

L

F

A

G

I

V

P

S

T

A

S

Y

S

S

K

K

A

Q

K

Q

D

D

A

E

A

A

K

K

V

K

F

L

T

V

S

E

W

Y

A

L

T

S

S

S

K

Q

E

D

Y

V

G

S

E

K

L

F

V

M

A

A

binding [(2~{S},3~{S},4~{S},5~{R},6~{S})-6-[(2~{S})-3-butanoyloxy-2-heptanoyloxy-propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: N40 (vs. gap), T41 (vs. gap), S42 (≠ E66), D66 (≠ G90), F109 (≠ Y138), D111 (≠ E140), E163 (≠ W192), G164 (= G193), A217 (= A243), T218 (≠ S244), D219 (≠ S245), W236 (≠ A266), G272 (≠ Y303), G273 (≠ S304), W274 (= W305)

Sites not aligning to the query:

binding [(2~{S},3~{S},4~{S},5~{R},6~{S})-6-[(2~{S})-3-butanoyloxy-2-heptanoyloxy-propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 9, 10, 343

7ofyA Crystal structure of sq binding protein from agrobacterium tumefaciens in complex with sulfoquinovosyl glycerol (sqgro) (see paper)
27% identity, 65% coverage: 50:336/440 of query aligns to 25:307/389 of 7ofyA

query
sites
7ofyA

F

Y

E

E

A

A

E

K

H

T

P

P

E

G

I

V

K

K

L

-

N

-

W

-

V

V

L

I

E

E

-

T

-

G

-

N

-
x
T

E
x
S

N

E

V

M

L

Q

R

A

Q

Q

R

Y

L

L

T

N

T

T

D

V

I

M

A

S

T

A

Q

K

G

D

G

S

Q

S

F

L

D

D

V

V

L

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

A

P

A

A

L

Q

W

F

G

A

A

T

K

A

G

G

W

W

L

T

E

S

P

D

M

F

K

-

D

-

L

-

P

-

A

S

S

G

Y

K

D

D

L

L

D

S

D

A

V

Y

F

L

P

P

S

T

V

Y

R

A

D

E

G

A

L

N

S

T

V

V

K

N

G

G

S

K

L

I

Y

V

A

A

L

L

P

P

F

A

Y

F

A

A

E
x
D

S

S

S

M

I

F

T

L

Y

Y

R

R

T

K

D

D

L

L

F

L

K

D

D

K

A

Y

G

G

L

I

T

K

M

P

P

P

E

-

H

-

P

T

T

T

W

W

T

D

Q

E

I

L

G

K

E

E

F

A

A

S

S

K

K

K

L

V

T

M

-

E

-

G

D

E

K

K

T

N

K

P

E

E

Q

L

Y

Q

G

G

L

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W

E

G
|
G

E

-

N

-

M

-

A

A

L

V

I

C

T

T

T

F

L

L

A

L

N

P

G

Y

Y

W

G

S

-

E

A

G

R

K

W

S

F

L

D

V

E

E

K

N

W

G

Q

K

P

L

E

N

F

F

N

D

G

N

P

K

E

A

W

A

K

V

D

D

A

S

L

L

N

K

F

L

-

W

-

K

-

S

Y

F

V

V

D

D

N

D

-

G

-

I

M

S

K

K

S

N

G

I

P

S

P

E

G

V

A

A

S
x
T

S

D

N

D

G

T

F

R

N

K

E

E

N

-

L

-

A

-

L

-

F

F

N

Q

S

A

G

G

K

K

C

V

A

L

I

F

W

A

V

V

D

N

A
x
W

S

S

V
x
Y

A

A

G

W

S

T

F

H

V

F

T

Q

D

G

K

K

T

-

Q

E

S

S

K

Q

V

V

A

N

D

D

H

K

V

V

G

G

F

V

T

A

F

R

A

L

P

P

H

A

E

V

K

K

T

G

D

G

K

E

G

Q

T

T

S

T

W

C

L

L

Y
x
G

S
x
G

W
|
W

S

E

L

F

A

G

I

V

P

S

T

A

S

Y

S

S

K

K

A

Q

K

Q

D

D

A

E

A

A

K

K

V

K

F

L

T

V

S

E

W

Y

A

L

T

S

S

S

K

Q

E

D

Y

V

G

S

E

K

L

F

V

M

A

A

binding [(2S,3S,4S,5R,6S)-6-[(2R)-2,3-bis(oxidanyl)propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: T42 (vs. gap), S43 (≠ E66), D67 (≠ G90), D113 (≠ E140), G166 (= G193), T220 (≠ S244), W238 (≠ A266), Y240 (≠ V268), G274 (≠ Y303), G275 (≠ S304), W276 (= W305)

Sites not aligning to the query:

binding [(2S,3S,4S,5R,6S)-6-[(2R)-2,3-bis(oxidanyl)propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 12, 345

7yzsAAA Sulfoquinovosyl binding protein (see paper)
27% identity, 65% coverage: 50:336/440 of query aligns to 23:305/384 of 7yzsAAA

query
sites
7yzsAAA

F

Y

E

E

A

A

E

K

H

T

P

P

E

G

I

V

K

K

L

-

N

-

W

-

V

V

L

I

E

E

-

T

-

G

-

N

-
x
T

E
x
S

N

E

V

M

L

Q

R

A

Q

Q

R

Y

L

L

T

N

T

T

D

V

I

M

A

S

T

A

Q

K

G

D

G

S

Q

S

F

L

D

D

V

V

L

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

A

P

A

A

L

Q

W

F

G

A

A

T

K

A

G

G

W

W

L

T

E

S

P

D

M

F

K

-

D

-

L

-

P

-

A

S

S

G

Y

K

D

D

L

L

D

S

D

A

V

Y

F

L

P

P

S

T

V

Y

R

A

D

E

G

A

L

N

S

T

V

V

K

N

G

G

S

K

L

I

Y

V

A

A

L

L

P

P

F

A

Y

F

A

A

E
x
D

S

S

S

M

I

F

T

L

Y

Y

R

R

T

K

D

D

L

L

F

L

K

D

D

K

A

Y

G

G

L

I

T

K

M

P

P

P

E

-

H

-

P

T

T

T

W

W

T

D

Q

E

I

L

G

K

E

E

F

A

A

S

S

K

K

K

L

V

T

M

-

E

-

G

D

E

K

K

T

N

K

P

E

E

Q

L

Y

Q

G

G

L

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W
x
E

G
|
G

E

-

N

-

M

-

A

A

L

V

I

C

T

T

T

F

L

L

A

L

N

P

G

Y

Y

W

G

S

-

E

A

G

R

K

W

S

F

L

D

V

E

E

K

N

W

G

Q

K

P

L

E

N

F

F

N

D

G

N

P

K

E

A

W

A

K

V

D

D

A

S

L

L

N

K

F

L

-

W

-

K

-

S

Y

F

V

V

D

D

N

D

-

G

-

I

M

S

K

K

S

N

G

I

P

S

P

E

G

V

A

A

S
x
T

S

D

N

D

G

T

F

R

N

K

E

E

N

-

L

-

A

-

L

-

F

F

N

Q

S

A

G

G

K

K

C

V

A

L

I

F

W

A

V

V

D

N

A
x
W

S

S

V

Y

A

A

G

W

S

T

F

H

V

F

T

Q

D

G

K

K

T

-

Q

E

S

S

K

Q

V

V

A

N

D

D

H

K

V

V

G

G

F

V

T

A

F

R

A

L

P

P

H

A

E

V

K

K

T

G

D

G

K

E

G

Q

T

T

S

T

W

C

L

L

Y
x
G

S
x
G

W
|
W

S

E

L

F

A

G

I

V

P

S

T

A

S

Y

S

S

K

K

A

Q

K

Q

D

D

A

E

A

A

K

K

V

K

F

L

T

V

S

E

W

Y

A

L

T

S

S

S

K

Q

E

D

Y

V

G

S

E

K

L

F

V

M

A

A

binding 6-deoxy-6-sulfo-beta-D-glucopyranose: T40 (vs. gap), S41 (≠ E66), D65 (≠ G90), D111 (≠ E140), E163 (≠ W192), G164 (= G193), T218 (≠ S244), W236 (≠ A266), G272 (≠ Y303), G273 (≠ S304), W274 (= W305)

Sites not aligning to the query:

binding 6-deoxy-6-sulfo-beta-D-glucopyranose: 11, 343

7yzuA Crystal structure of the sulfoquinovosyl binding protein smof complexed with sqme (see paper)
26% identity, 65% coverage: 50:336/440 of query aligns to 22:302/382 of 7yzuA

query
sites
7yzuA

F

Y

E

E

A

A

E

K

H

T

P

P

E

G

I

V

K

K

L

-

N

-

W

-

V

V

L

I

E

E

-

T

-

G

-

N

-
x
T

E
x
S

N

E

V

M

L

Q

R

A

Q

Q

R

Y

L

L

T

N

T

T

D

V

I

M

A

S

T

A

Q

K

G

D

G

S

Q

S

F

L

D

D

V

V

L

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

A

P

A

A

L

Q

W

F

G

A

A

T

K

A

G

G

W

W

L

T

E

S

P

D

M

F

K

-

D

-

L

-

P

-

A

S

S

G

Y

K

D

D

L

L

D

S

D

A

V

Y

F

L

P

P

S

T

V

Y

R

A

D

E

G

A

L

N

S

T

V

V

K

N

G

G

S

K

L

I

Y

V

A

A

L

L

P

P

F

A

Y

F

A

A

E
x
D

S

S

S

M

I

F

T

L

Y

Y

R

R

T

K

D

D

L

L

F

L

K

D

D

K

A

Y

G

G

L

I

T

K

M

P

P

P

E

-

H

-

P

T

T

T

W

W

T

D

Q

E

I

L

G

K

E

E

F

A

A

S

S

K

K

K

L

V

T

M

-

E

-

G

D

E

K

K

T

N

K

P

E

E

Q

L

Y

Q

G

G

L

L

C

S

L

F

R

Q

G

G

K

K

A

A

-

I

-
x
E

-
x
G

-

A

-

V

-

C

-

T

-

F

-

L

-

P

G

Y

W

W

G

S

E

E

N

G

M

K

A

S

L

L

I

V

T

G

T

K

L

L

A

N

N

-

G

-

Y

-

G

-

A

-

R

-

W

-

F

F

D

D

E

N

K

K

W

A

Q

A

P

V

E

D

F

-

N

S

G

L

P

K

E

L

W

W

K

K

D

-

A

-

L

-

N

S

F

F

Y

V

V

D

D

D

N

G

M

I

K

S

K

K

S

K

G

N

P

I

P

S

G

E

A

V

S

A

S
x
T

N

D

G

D

F

T

N

R

E

K

N

E

L

-

A

-

L

-

F

F

N

Q

S

A

G

G

K

K

C

V

A

L

I

F

W

A

V

V

D

N

A
x
W

S

S

V

Y

A

A

G

W

S

T

F

H

V

F

T

Q

D

G

K

K

T

-

Q

E

S

S

K

Q

V

V

A

N

D

D

H

K

V

V

G

G

F

V

T

A

F

R

A

L

P

P

H

A

E

V

K

K

T

G

D

G

K

E

G

Q

T

T

S

T

W

C

L

L

Y
x
G

S
x
G

W
|
W

S

E

L

F

A

G

I

V

P

S

T

A

S

Y

S

S

K

K

A

Q

K

Q

D

D

A

E

A

A

K

K

V

K

F

L

T

V

S

E

W

Y

A

L

T

S

S

S

K

Q

E

D

Y

V

G

S

E

K

L

F

V

M

A

A

binding [(2S,3S,4S,5R,6S)-6-methoxy-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: T39 (vs. gap), S40 (≠ E66), D64 (≠ G90), D110 (≠ E140), E162 (vs. gap), G163 (vs. gap), T215 (≠ S245), W233 (≠ A266), G269 (≠ Y303), G270 (≠ S304), W271 (= W305)

Sites not aligning to the query:

binding [(2S,3S,4S,5R,6S)-6-methoxy-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 9, 340

8s5bA Sulfoquinovosyl glycerol-binding protein SmoF (see paper)
28% identity, 65% coverage: 50:336/440 of query aligns to 18:297/377 of 8s5bA

query
sites
8s5bA

F

Y

E

E

A

A

E

K

H

T

P

P

E

G

I

V

K

K

L

-

N

-

W

-

V

V

L

I

E

E

-

T

-

G

-

N

-
x
T

E
x
S

N

E

V

M

L

Q

R

A

Q

Q

R

Y

L

L

T

N

T

T

D

V

I

M

A

S

T

A

Q

K

G

D

G

S

Q

S

F

L

D

D

V

V

L

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

A

P

A

A

L

Q

W

F

G

A

A

T

K

A

G

G

W

W

L

T

E

S

P

D

M

F

K

-

D

-

L

-

P

-

A

S

S

G

Y

K

D

D

L

L

D

S

D

A

V

Y

F

L

P

P

S

T

V

Y

R

A

D

E

G

A

L

N

S

T

V

V

K

N

G

G

S

K

L

I

Y

V

A

A

L

L

P

P

F

A

Y
x
F

A

A

E
x
D

S

S

S

M

I

F

T

L

Y

Y

R

R

T

K

D

D

L

L

F

L

K

D

D

K

A

Y

G

G

L

I

T

K

M

P

P

P

E

-

H

-

P

T

T

T

W

W

T

D

Q

E

I

L

G

K

E

E

F

A

A

S

S

K

K

K

L

V

T

M

-

E

-

G

D

E

K

K

T

N

K

P

E

E

Q

L

Y

Q

G

G

L

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W
x
E

G
|
G

E

-

N

-

M

-

A

A

L

V

I

C

T

T

T

F

L

L

A

L

N

-

G

-

Y

-

G

-

A

P

R

Y

W

W

F

S

D

E

E

G

K

K

-

S

-

L

W

V

Q

K

P

L

E

N

F

F

N

D

G

N

P

K

E

A

W

A

K

V

D

D

A

S

L

L

N

K

F

L

-

W

-

K

-

S

Y

F

V

V

D

D

N

D

-

G

-

I

M

S

K

K

S

N

G

I

P

S

P

E

G

V

A

A

S
x
T

S

D

N

D

G

T

F

R

N

K

E

E

N

-

L

-

A

-

L

-

F

F

N

Q

S

A

G

G

K

K

C

V

A

L

I

F

W

A

V

V

D

N

A
x
W

S

S

V
x
Y

A

A

G

W

S

T

F

H

V

F

T

Q

D

G

K

K

T

-

Q

E

S

S

K

Q

V

V

A

N

D

D

H

K

V

V

G

G

F

V

T

A

F

R

A

L

P

P

H

A

E

V

K

K

T

G

D

G

K

E

G

Q

T

T

S

T

W

C

L

L

Y
x
G

S
x
G

W
|
W

S

E

L

F

A

G

I

V

P

S

T

A

S

Y

S

S

K

K

A

Q

K

Q

D

D

A

E

A

A

K

K

V

K

F

L

T

V

S

E

W

Y

A

L

T

S

S

S

K

Q

E

D

Y

V

G

S

E

K

L

F

V

M

A

A

binding [(2~{S},3~{S},4~{S},5~{R},6~{S})-6-octoxy-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: T35 (vs. gap), S36 (≠ E66), D60 (≠ G90), F104 (≠ Y138), D106 (≠ E140), E158 (≠ W192), G159 (= G193), T210 (≠ S244), W228 (≠ A266), Y230 (≠ V268), G264 (≠ Y303), G265 (≠ S304), W266 (= W305)

Sites not aligning to the query:

binding [(2~{S},3~{S},4~{S},5~{R},6~{S})-6-octoxy-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 335

5ysdB Crystal structure of beta-1,2-glucooligosaccharide binding protein in complex with sophorotriose (see paper)
25% identity, 83% coverage: 70:435/440 of query aligns to 42:381/389 of 5ysdB

query
sites
5ysdB

R

H

Q

D

R

K

L

L

T

L

T

T

D

A

I

V

A

A

T

S

Q

K

G

S

G

G

Q

P

F

-

D

D

V

V

L

V

T

Q

I

M

G
|
G

-
x
T

-
x
W

M

M

Y

P

E

E

A

F

A

V

L

E

W

A

G

G

A

A

K

-

G

-

W

L

L

L

E

D

P

I

M

T

K

K

D

D

L

V

P

E

A

K

S

S

Y

K

D

N

L

M

-

N

-

S

D

D

D

L

V

F

F

F

P

P

S

G

V

S

R

V

D

K

G

T

L

T

S

Q

V

F

K

D

G

G

S

K

L

T

Y

Y

A

G

L

V

P

P

F

W

Y
|
Y

A

A

E
|
E

S

T

S

R

I

V

T

L

Y

F

Y

Y

R

R

T

T

D

D

L

L

F

L

K

K

D

K

A

V

G

G

L

Y

T

N

M

-

P

-

E

E

H

A

P

P

-

K

T

T

W

W

T

D

Q

E

I

L

G

S

E

D

F

A

A

A

S

L

K

K

L

L

T

S

D

K

K

R

T

G

K

K

E

D

Q

M

Y

Y

G

G

L

F

C

A

L

I

R
x
D

G

P

K

N

A

E

G

-

W

-

G

-

E
x
Q

N

T

M

T

A

G

L

F

I

I

T

F

T

G

L

R

A

Q

N

N

G

-

Y

-

G

G

A

S

R

P

W

L

F

F

D

D

E

K

K

D

W

G

Q

T

P

P

E

V

F

F

N

N

G

K

P

K

E

P

W

F

K

V

D

D

A

T

L

V

N

T

F

-

Y

Y

V

L

D

D

N

S

M

F

K

I

K

K

S

N

G

G

P

S

P

A

G

P

A

D

S

T

S

D

N

L

G

G

F

L

N

D

E

A

N

S

L

Q

A

S

L

F

F

G

N

G

S

D

G

G

K

I

C

V

A

P

I

M

W

F

V

-

D

-

A

-

S

-

V

M

A

S

G

G

S

P

F
x
W

V

M

T

V

D

N

K

T

T

L

Q

K

S

D

K

T

V

A

A

P

D

D

H

I

V

D

G

G

-

K

F

W

T

A

F

T

A

A

P

V

H

L

E

P

K

K

T

K

D

E

K

N

G

N

T

E

S

S

W

S

L

L

Y

G

S
x
G

W

A

S

N

L

L

A

S

I

I

P

F

T

K

S

Y

S

S

K

N

A

K

K

K

D

D

A

D

A

A

K

L

V

K

F

F

T

M

S

D

W

Y

A

M

T

S

S

Q

K

P

E

D

Y

V

G

-

E

Q

L

L

V

S

A

W

K

L

T

K

D

D

G

-

I

-

A

T

N

N

V

S

P

M

P

P

G

A

T

-

R

R

K

M

S

D

T

A

Y

W

N

E

D

D

E

D

Y

M

M

L

K

K

A

N

A

D

P

P

F

Y

A

Y

K

K

V

V

T

F

L

G

E

E

S

Q

L

M

K

K

V

T

A

A

D

E

P

P

T

-

K

-

P

-

T

-

L

-

K

-

P

-

V

-

P

-

Y

-

I

-

G

-

I

-

Q

-

L

M

V

P

T

L

I

I

P

P

E

Q

F

F

Q

E

A

E

I

I

G

A

T

Q

Q

L

V

Y

G

G

K

K

F

S

F

W

S

E

G

Q

A

I

L

Y

T

R

G

G

Q

G

Q

A

T

D

V

V

D

Q

A

T

A

Q

L

M

T

D

A

T

A

F

Q

N

T

D

T

Q

T

V

E

E

R

A

E

L

M

L

K

K

R

K

binding beta-D-glucopyranose: G61 (= G90), T62 (vs. gap), T63 (vs. gap), W64 (vs. gap), Y112 (= Y138), E114 (= E140), W235 (≠ F272), G268 (≠ S304)
binding alpha-D-glucopyranose: D160 (≠ R187), Q164 (≠ E194)

Sites not aligning to the query:

5ysbA Crystal structure of beta-1,2-glucooligosaccharide binding protein in ligand-free form (see paper)
25% identity, 83% coverage: 70:435/440 of query aligns to 41:380/386 of 5ysbA

query
sites
5ysbA

R
x
H

Q

D

R

K

L

L

T

L

T

T

D

A

I

V

A

A

T

S

Q

K

G

S

G

G

Q

P

F

-

D

D

V

V

L

V

T

Q

I

M

G

G

-

T

-

W

M

M

Y

P

E
|
E

A

F

A

V

L
x
E

W

A

G

G

A

A

K

-

G

-

W

L

L

L

E

D

P

I

M

T

K

K

D

D

L

V

P

E

A

K

S

S

Y

K

D

N

L

M

-

N

-

S

D

D

D

L

V

F

F

F

P

P

S

G

V

S

R

V

D

K

G

T

L

T

S

Q

V

F

K

D

G

G

S

K

L

T

Y

Y

A

G

L

V

P

P

F

W

Y

Y

A

A

E

E

S

T

S

R

I

V

T

L

Y

F

Y

Y

R

R

T

T

D

D

L

L

F

L

K

K

D

K

A

V

G

G

L

Y

T

N

M

-

P

-

E

E

H

A

P

P

-

K

T

T

W

W

T

D

Q

E

I

L

G

S

E

D

F

A

A

A

S

L

K

K

L

L

T

S

D

K

K

R

T

G

K

K

E

D

Q

M

Y

Y

G

G

L

F

C

A

L

I

R

D

G

P

K

N

A

E

G

-

W

-

G

-

E

Q

N

T

M

T

A

G

L

F

I

I

T

F

T

G

L

R

A

Q

N

N

G

-

Y

-

G

G

A

S

R

P

W

L

F

F

D

D

E

K

K

D

W

G

Q

T

P

P

E

V

F

F

N

N

G

K

P

K

E

P

W

F

K

V

D

D

A

T

L

V

N

T

F

-

Y

Y

V

L

D

D

N

S

M

F

K

I

K

K

S

N

G

G

P

S

P

A

G

P

A

D

S

T

S

D

N

L

G

G

F

L

N

D

E

A

N

S

L

Q

A

S

L

F

F

G

N

G

S

D

G

G

K

I

C

V

A

P

I

M

W

F

V

-

D

-

A

-

S

-

V

M

A

S

G

G

S

P

F

W

V

M

T

V

D
x
N

K

T

T

L

Q

K

S
x
D

K

T

V

A

A

P

D

D

H

I

V

D

G

G

-

K

F

W

T

A

F

T

A

A

P

V

H

L

E

P

K

K

T

K

D

E

K

N

G

N

T

E

S

S

W

S

L

L

Y

G

S

G

W

A

S

N

L

L

A

S

I

I

P

F

T

K

S

Y

S

S

K

N

A

K

K

K

D

D

A

D

A

A

K

L

V

K

F

F

T

M

S

D

W

Y

A

M

T

S

S

Q

K

P

E

D

Y

V

G

-

E

Q

L

L

V

S

A

W

K

L

T

K

D
|
D

G

-

I

-

A

T

N

N

V

S

P

M

P

P

G

A

T

-

R

R

K

M

S

D

T

A

Y

W

N

E

D

D

E

D

Y

M

M

L

K

K

A

N

A

D

P

P

F

Y

A

Y

K

K

V

V

T

F

L

G

E

E

S

Q

L

M

K

K

V

T

A

A

D

E

P

P

T

-

K

-

P

-

T

-

L

-

K

-

P

-

V

-

P

-

Y

-

I

-

G

-

I

-

Q

-

L

M

V

P

T

L

I

I

P

P

E

Q

F

F

Q

E

A

E

I

I

G

A

T

Q

Q

L

V

Y

G

G

K

K

F

S

F

W

S

E

G

Q

A

I

L

Y

T

R

G

G

Q

G

Q

A

T

D

V

V

D

Q

A

T

A

Q

L

M

T

D

A

T

A

F

Q

N

T

D

T

Q

T

V

E

E

R

A

E

L

M

L

K

K

R

K

binding zinc ion: H41 (≠ R70), E66 (= E93), E69 (≠ L96), N237 (≠ D275), D241 (≠ S279), D301 (= D339)

Sites not aligning to the query:

binding zinc ion: 17, 384, 385

5ci5A Crystal structure of an abc transporter solute binding protein from thermotoga lettingae tmo (tlet_1705, target efi-510544) bound with alpha-d-tagatose
24% identity, 75% coverage: 100:431/440 of query aligns to 75:389/393 of 5ci5A

query
sites
5ci5A

K

K

G

G

W

W

L

L

E

E

P

P

M

M

K

E

D

E

L

H

P

L

A

K

S

N

Y

W

D

E

-

G

L

T

D

A

D

Q

V

I

F

W

P

P

S

N

V

L

R

W

D

P

G

T

L

V

S

T

V

Y

K

K

G

K

S

I

L

R

Y

Y

A

G

L

L

P

P

F

W

Y
|
Y

A

T

E
x
D

S

C

S
x
R

I

L

T

L

Y

L

Y

Y

R

N

T

K

D

A

L

M

F

F

K

E

D

K

A

A

G

G

L

L

T

N

M

-

P

P

E

D

H

N

P

P

-

P

-

K

T

T

W

W

T

D

Q

E

I

L

G

L

E

D

F

A

A

A

S

L

K

K

L

I

T

T

D

D

K

T

T

K

K

N

E

R

Q

I

Y

Y

G

G

L

Y

C

G

L

V

R

S

G

G

K

T

A

K

G

T

W

E

G

H

E

T

N

T

M

L

A

G

L

Y

I

M

T

M

T

F

L

L

A

Y

N

A

G

A

Y

G

G

G

A

K

R

L

W

L

F

T

D

D

E

D

K

Y

W

S

Q

K

P

A

E

A

F

F

N

D

G

S

P

P

E

E

W

G

K

L

D

K

A

A

L

L

N

K

F

F

Y

Y

V

T

D

D

N

L

M

A

K

K

S

Y

G

-

P

-

G

N

A

V

S

S

S

P

N

N

G

A

F

I

N

Q

E

Y

N

H

L

E

A

D

L

D

F

Y

N

R

S

N

G

M

K

M

C

A

A

Q

I

N

W

R

V

V

D

A

A

M

S

A

V

I

A

G

G

G

S

P

F
x
W

V

S

T

F

D

P

K

L

T

I

Q

E

S

A

K

A

V

N

A

P

D

D

H

I

V

A

G

G

-

K

F

Y

T

S

F

V

A

A

P

L

H

H

E

P

K

Y

T

D

D

A

K

K

G

P

T

A

S

S

W

V

L

L

Y
x
G

S
x
G

W
|
W

S

A

L

L

A

V

I

I

P

P

T

S

S

S

K

P

A

N

K

K

D

E

A

D

A

A

K

W

V

K

F

L

T

A

S

E

W

Y

A

L

T

T

S

S

K

F

E

D

-

V

Y

W

G

M

E

K

L

W

V

V

A

E

K

E

T

K

D

G

G

G

I

-

A

-

N

-

V

-

P

P

P

M

G

P

T

T

R

R

K

M

S

D

T

V

Y

C

N

K

D

K

E

S

Y

K

M

L

K

A

A

N