SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2540 FitnessBrowser__WCS417:GFF2540 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

A0A0H2ZQB9 Ergothioneine transporter EgtUBC from Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (see paper)
30% identity, 57% coverage: 116:270/273 of query aligns to 61:212/506 of A0A0H2ZQB9

query
sites
A0A0H2ZQB9

L

F

R

Q

S

T

I

I

P

P

S

S

L

L

A

A

W

L

V

L

P

G

L

L

L

F

L

I

L

P

W

L

L

M

G

G

I

I

D

G

E

T

T

L

S

P

K

A

I

L

V

T

L

A

I

L

A

V

I

I

G

Y

A

A

F

I

F

F

P

P

V

I

Y

L

L

Q

N

N

G

T

V

I

A

T

A

G

I

L

R

K

D

G

I

I

D

D

R

P

K

S

L

L

V

Q

E

E

V

A

G

G

Q

I

M

A

Y

F

G

G

F

M

S

T

R

R

W

W

R

E

L

R

V

L

R

K

R

K

I

F

L

E

L

I

P

P

A

L

A

A

L

M

P

P

G

V

L

I

F

M

T

S

G

G

L

I

R

R

S

T

G

A

L

A

S

V

L

L

A

I

W

I

M

G

F

T

L

A

V

T

A

L

A

A

E

T

L

L

I

I

A

G

A

A

T

G

K

-

G

G

L

L

G

G

Y

S

L

F

L

I

S

L

D

L

G

G

R

I

E

D

T

R

S

N

R

N

P

A

D

S

I

L

V

I

L

L

A

I

A

G

I

A

I

L

V

S

L

S

A

A

S

V

L

L

G

A

K

I

V

A

S

F

D

N

G

F

L

L

L

L

A

K

T

V

L

M

E

E

K

K

R

A

C

K

L

L

A

-

W

-

R

R

D

T

T

I

F

F

Q

S

G

G

Sites not aligning to the query:

244 G→F: Disrupts ergothioneine (EGT) binding. Binds EGT 100-fold more weakly. May have a global impact on overall structure.
293 F→Y: Disrupts ergothioneine (EGT) binding.
375 E→Q: Disrupts ergothioneine (EGT) binding. Binds EGT 500-fold more weakly.
419 Y→F: Disrupts ergothioneine (EGT) binding.

Query Sequence

>GFF2540 FitnessBrowser__WCS417:GFF2540
MTSKTQALPLVEPVAINRSAWPRRLKGLVLPVLILLVLEVVVRVGWLPSYQMPAPSEIAV
TLTDLAEGSLWKHISASLGRVLLGFAIGASLALVFAAWVGLSREAEAYLEPTFAGLRSIP
SLAWVPLLLLWLGIDETSKIVLIAIGAFFPVYLNGVAAIRDIDRKLVEVGQMYGFSRWRL
VRRILLPAALPGLFTGLRSGLSLAWMFLVAAELIAATKGLGYLLSDGRETSRPDIVLAAI
IVLASLGKVSDGLLATLEKRCLAWRDTFQGAGQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory