SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF260 FitnessBrowser__WCS417:GFF260 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ho9A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-galactose and utp
70% identity, 98% coverage: 2:287/291 of query aligns to 1:286/294 of 4ho9A

query
sites
4ho9A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

H

Y

P

P

I

L

T

T

L

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L
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L

A

A

G

G

I

I

K

K

D

D

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

E

D

D

L

T

P

P

Q

R

Y

F

R

E

N

Q

L

L

G

G

D

G

G

G

S

S

Q

E

F

L

G

G

V

I

R

S

F

L

S

S

Y

Y

A

A

E

V

Q
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Q

P

S

T

S

P
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P

D

D

G
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G

L
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L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

D

D

P

N

V

V

C

A

L

L

I

V

L
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L

G

G

D
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D

N

N

I

I

F

F

H

Y

G
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G

Q
x
H

H
x
G

F

F

G
x
T

E

E

Q

L

L

L

L

Q

G

R

A

A

K

N

R

R

A

K

S

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

K

K

D

D

P

P

E

Q

R

R

F

F

G
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G

V

V

I

V

D

E

F

F

D

D

S

E

E

K

G

G

R

K

A

V

L

I

S

S

I

I

E

E

E
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E

K
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K

P

P

A

E

K

E

P

P

K

K

S

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

D

R

V

V

I

V

K

D

I

I

A

A

K

K

A

N

V

I

K

T

P

P

S

S

P

A

R
|
R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

R

L

G

G

D

E

L

L

H

H

V

V

E

E

R

L

F

L

G
|
G

R
|
R

G
|
G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

E

Q

A

A

S

S

Q

Q

Y

F

V

I

Q

E

T

T

I

I

E

E

H

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

V
x
I

A

A

Y

Y

E

R

N

M

G

G

W

Y

I

I

N

S

R

R

E

E

H

Q

L

L

L

I

E

K

R

L

A

A

K

E

Y

P

F

L

G

M

K

K

T

N

G

E

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

M

S

N

L

L

A

A

binding galactose-uridine-5'-diphosphate: L6 (= L7), G8 (= G9), G9 (= G10), Q80 (= Q81), P83 (= P84), G85 (= G86), L86 (= L87), L106 (= L107), D108 (= D109), G144 (= G145), E159 (= E160), K160 (= K161), V170 (= V171), R192 (= R193), W221 (= W222)
binding uridine 5'-triphosphate: L43 (= L44), G113 (= G114), H114 (≠ Q115), G115 (≠ H116), T117 (≠ G118), G216 (= G217), R217 (= R218), G218 (= G219), I254 (≠ V255)

4ho4A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine and glucose-1-phosphate
70% identity, 98% coverage: 2:287/291 of query aligns to 1:286/289 of 4ho4A

query
sites
4ho4A

M

M

K

K

G

G

I

I

V

I

L

L

A

S

G

G

S

S

G

G

T

T

R

R

L

L

H

Y

P

P

I

L

T

T

L

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

K

K

D

D

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

E

D

D

L

T

P

P

Q

R

Y

F

R

E

N

Q

L

L

G

G

D

G

G

G

S

S

Q

E

F

L

G

G

V

I

R

S

F

L

S

S

Y

Y

A

A

E

V

Q

Q

P

S

T

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

D

D

P

N

V

V

C

A

L

L

I

V

L
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L

G

G

D

D

N

N

I

I

F

F

H

Y

G

G

Q

H

H

G

F

F

G

T

E

E

Q

L

L

L

L

Q

G

R

A

A

K

N

R

R

A

K

S

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

K

K

D

D

P

P

E

Q

R

R

F
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F

G
|
G

V

V

I

V

D

E

F

F

D

D

S

E

E

K

G

G

R

K

A

V

L

I

S

S

I

I

E

E

E
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E

K
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K

P

P

A

E

K

E

P

P

K

K

S

S

Y

Y

A

A

V
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V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

D

R

V

V

I

V

K

D

I

I

A

A

K

K

A

N

V

I

K

T

P

P

S

S

P

A

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

R

L

G

G

D

E

L

L

H

H

V

V

E

E

R

L

F

L

G

G

R

R

G

G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

E

Q

A

A

S

S

Q

Q

Y

F

V

I

Q

E

T

T

I

I

E

E

H

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

V

I

A

A

Y

Y

E

R

N

M

G

G

W

Y

I

I

N

S

R

R

E

E

H

Q

L

L

L

I

E

K

R

L

A

A

K

E

Y

P

F

L

G

M

K

K

T

N

G

E

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

M

S

N

L

L

A

A

binding 1-O-phosphono-alpha-D-glucopyranose: L106 (= L107), F143 (= F144), G144 (= G145), E159 (= E160), K160 (= K161), V170 (= V171), W221 (= W222)

4ho6A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-glucose and utp
70% identity, 98% coverage: 2:287/291 of query aligns to 1:286/288 of 4ho6A

query
sites
4ho6A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

H

Y

P

P

I

L

T

T

L

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L
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L

A

A

G

G

I

I

K

K

D

D

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

E

D

D

L

T

P

P

Q

R

Y

F

R

E

N

Q

L

L

G

G

D

G

G

G

S

S

Q

E

F

L

G

G

V

I

R

S

F

L

S

S

Y

Y

A

A

E

V

Q
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Q

P

S

T

S

P
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P

D

D

G
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G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

D

D

P

N

V

V

C

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

H
x
Y

G
|
G

Q
x
H

H
x
G

F

F

G
x
T

E

E

Q

L

L

L

L

Q

G

R

A

A

K

N

R

R

A

K

S

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

K

K

D

D

P

P

E

Q

R

R

F

F

G
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G

V

V

I

V

D

E

F

F

D

D

S

E

E

K

G

G

R

K

A

V

L

I

S

S

I

I

E

E

E
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E

K
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K

P

P

A

E

K

E

P

P

K

K

S

S

Y

Y

A

A

V
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V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

D

R

V

V

I

V

K

D

I

I

A

A

K

K

A

N

V

I

K

T

P

P

S

S

P

A

R
|
R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

R

L

G

G

D

E

L

L

H

H

V

V

E

E

R

L

F

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

E

Q

A

A

S

S

Q

Q

Y

F

V

I

Q

E

T

T

I

I

E

E

H

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

E
|
E

V
x
I

A

A

Y

Y

E

R

N

M

G

G

W

Y

I

I

N

S

R

R

E

E

H

Q

L

L

L

I

E

K

R

L

A

A

K

E

Y

P

F

L

G

M

K

K

T

N

G

E

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

M

S

N

L

L

A

A

binding uridine-5'-diphosphate-glucose: L6 (= L7), G8 (= G9), G9 (= G10), Q80 (= Q81), P83 (= P84), G85 (= G86), D108 (= D109), G144 (= G145), E159 (= E160), K160 (= K161), V170 (= V171), R192 (= R193)
binding uridine 5'-triphosphate: L43 (= L44), Y112 (≠ H113), G113 (= G114), H114 (≠ Q115), G115 (≠ H116), T117 (≠ G118), E253 (= E254), I254 (≠ V255)

4ho5A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with tdp-glucose
70% identity, 98% coverage: 2:287/291 of query aligns to 1:286/288 of 4ho5A

query
sites
4ho5A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

H

Y

P

P

I

L

T

T

L

K

G

V

V

V

S

S

K

K

Q
|
Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

K

K

D

D

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

E

D

D

L

T

P

P

Q

R

Y

F

R

E

N

Q

L

L

G

G

D

G

G

G

S

S

Q

E

F

L

G

G

V

I

R

S

F

L

S

S

Y

Y

A

A

E

V

Q
|
Q

P

S

T

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

D

D

P

N

V

V

C

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

H
x
Y

G
|
G

Q
x
H

H
x
G

F
|
F

G
x
T

E

E

Q

L

L

L

L

Q

G

R

A

A

K

N

R

R

A

K

S

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

K

K

D

D

P

P

E

Q

R

R

F
|
F

G
|
G

V

V

I

V

D

E

F

F

D

D

S

E

E

K

G

G

R

K

A

V

L

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

A

E

K

E

P

P

K

K

S

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

D

R

V

V

I

V

K

D

I

I

A

A

K

K

A

N

V

I

K

T

P

P

S

S

P

A

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

R

L

G

G

D

E

L

L

H

H

V

V

E

E

R

L

F

L

G
|
G

R
|
R

G
|
G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

E

Q

A

A

S

S

Q

Q

Y

F

V

I

Q

E

T

T

I

I

E

E

H

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

E
|
E

V
x
I

A

A

Y

Y

E

R

N

M

G

G

W

Y

I

I

N

S

R

R

E

E

H

Q

L

L

L

I

E

K

R

L

A

A

K

E

Y

P

F

L

G

M

K

K

T

N

G

E

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

M

S

N

L

L

A

A

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L6 (= L7), G8 (= G9), G9 (= G10), Q24 (= Q25), Q80 (= Q81), P83 (= P84), G85 (= G86), L86 (= L87), L106 (= L107), D108 (= D109), Y112 (≠ H113), G113 (= G114), H114 (≠ Q115), G115 (≠ H116), F116 (= F117), T117 (≠ G118), F143 (= F144), G144 (= G145), E159 (= E160), K160 (= K161), V170 (= V171), G216 (= G217), R217 (= R218), G218 (= G219), W221 (= W222), E253 (= E254), I254 (≠ V255)

4ho3A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine triphosphate
70% identity, 98% coverage: 2:287/291 of query aligns to 1:286/288 of 4ho3A

query
sites
4ho3A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G
|
G

T
|
T

R
|
R

L

L

H

Y

P

P

I

L

T

T

L

K

G

V

V

V

S

S

K

K

Q
|
Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L
|
L

A

A

G

G

I

I

K

K

D

D

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

E

D

D

L

T

P

P

Q

R

Y

F

R

E

N

Q

L

L

G

G

D

G

G

G

S

S

Q

E

F

L

G

G

V

I

R

S

F

L

S

S

Y

Y

A

A

E

V

Q
|
Q

P

S

T

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

D

D

P

N

V

V

C

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

H
x
Y

G
|
G

Q
x
H

H
x
G

F
|
F

G
x
T

E

E

Q

L

L

L

L

Q

G

R

A

A

K

N

R

R

A

K

S

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

K

K

D

D

P

P

E

Q

R

R

F

F

G

G

V

V

I

V

D

E

F

F

D

D

S

E

E

K

G

G

R

K

A

V

L

I

S

S

I

I

E

E

K

K

P

P

A

E

K

E

P

P

K

K

S

S

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

D

R

V

V

I

V

K

D

I

I

A

A

K

K

A

N

V

I

K

T

P

P

S

S

P

A

R

R

G

G

E
|
E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

R

L

G

G

D

E

L

L

H

H

V

V

E

E

R

L

F

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

E

Q

A

A

S

S

Q

Q

Y

F

V

I

Q

E

T

T

I

I

E

E

H

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

E
|
E

V
x
I

A

A

Y

Y

E
x
R

N

M

G

G

W

Y

I

I

N

S

R

R

E

E

H

Q

L

L

L

I

E

K

R

L

A

A

K

E

Y

P

F

L

G

M

K

K

T

N

G

E

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

M

S

N

L

L

A

A

binding thymidine-5'-triphosphate: L6 (= L7), G8 (= G9), G9 (= G10), G11 (= G12), T12 (= T13), R13 (= R14), Q24 (= Q25), L43 (= L44), Q80 (= Q81), P83 (= P84), G85 (= G86), L86 (= L87), D108 (= D109), Y112 (≠ H113), G113 (= G114), H114 (≠ Q115), G115 (≠ H116), F116 (= F117), T117 (≠ G118), E194 (= E195), E253 (= E254), I254 (≠ V255), R257 (≠ E258)

4hocA Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-n- acetylglucosamine
70% identity, 98% coverage: 2:287/291 of query aligns to 1:284/286 of 4hocA

query
sites
4hocA

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

H

Y

P

P

I

L

T

T

L

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

K

K

D

D

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

E

D

D

L

T

P

P

Q

R

Y

F

R

E

N

Q

L

L

G

G

D

G

G

G

S

S

Q

E

F

L

G

G

V

I

R

S

F

L

S

S

Y

Y

A

A

E

V

Q
|
Q

P

S

T

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

D

D

P

N

V

V

C

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

H

Y

G

G

Q

H

H

G

F

F

G

T

E

E

Q

L

L

L

L

Q

G

R

A

A

K

N

R

R

A

K

S

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

K

K

D

D

P

P

E

Q

R

R

F

F

G
|
G

V

V

I

V

D

E

F

F

D

D

S

E

E

K

G

G

R

K

A

V

L

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

A

E

K

E

P

P

K

K

S

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

D

R

V

V

I

V

K

D

I

I

A

A

K

K

A

N

V

I

K

T

P

P

S

S

P

-

R

-

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

R

L

G

G

D

E

L

L

H

H

V

V

E

E

R

L

F

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

E

Q

A

A

S

S

Q

Q

Y

F

V

I

Q

E

T

T

I

I

E

E

H

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

V

I

A

A

Y

Y

E

R

N

M

G

G

W

Y

I

I

N

S

R

R

E

E

H

Q

L

L

L

I

E

K

R

L

A

A

K

E

Y

P

F

L

G

M

K

K

T

N

G

E

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

M

S

N

L

L

A

A

binding uridine-diphosphate-n-acetylglucosamine: L6 (= L7), G8 (= G9), G9 (= G10), Q80 (= Q81), P83 (= P84), G85 (= G86), L86 (= L87), D108 (= D109), G144 (= G145), E159 (= E160), K160 (= K161), V170 (= V171)

P61887 Glucose-1-phosphate thymidylyltransferase 2; G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2; EC 2.7.7.24 from Escherichia coli (strain K12) (see paper)
67% identity, 98% coverage: 2:286/291 of query aligns to 1:285/293 of P61887

query
sites
P61887

M

M

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

I

T

T

L

R

G

G

V

V

S

S

K

K

Q

Q

L

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

T

T

T

P

P

V

E

D

D

L

K

P

G

Q

Y

Y

F

R

Q

N

R

L

L

G

G

D

D

G

G

S

S

Q

E

F

F

G

G

V

I

R

Q

F

L

S

E

Y

Y

A

A

E

E

Q

Q

P

P

T

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

E

T

F

F

I

L

G

N

D

G

D

E

P

P

V

S

C

C

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

H

F

G

G

Q

Q

H

G

F

F

G

S

E

P

Q

K

L

L

L

R

G

H

A

V

A

A

K

A

R

R

A

T

S

E

G

G

A

A

T

T

V

V

F

F

G

G

Y

Y

W

Q

V

V

K

M

D

D

P

P

E

E

R

R

F

F

G

G

V

V

I

V

D

E

F

F

D

D

S

D

E

N

G

F

R

R

A

A

L

I

S

S

I

L

E

E

K

K

P

P

A

K

K

Q

P

P

K

K

S

S

S

N

Y

W

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

S

D

K

V

V

I

V

K

E

I

Y

A

A

K

K

A

Q

V

V

K

K

P

P

S

S

P

E

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

S

V

I

N

N

N

Q

A

M

Y

Y

L

L

K

E

R

A

G

G

D

N

L

L

H

T

V

V

E

E

R

L

F

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

L

I

E

E

A

A

S

S

Q

T

Y

F

V

V

Q

Q

T

T

I

V

E

E

H

K

R

R

Q

Q

G

G

L

F

K

K

V

I

A

A

C

C

L

L

E

E

V

I

A

A

Y

W

E

R

N

N

G

G

W

W

I

L

N

D

R

D

E

E

H

G

L

V

L

K

E

R

R

A

A

A

K

S

Y

S

F

L

G

A

K

K

T

T

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

L

S

E

L

L

D108 (= D109) binding
D223 (= D224) binding

1mc3A Crystal structure of rffh (see paper)
67% identity, 98% coverage: 2:286/291 of query aligns to 2:286/291 of 1mc3A

query
sites
1mc3A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

S

S

G
|
G

T
|
T

R
|
R

L

L

H

H

P

P

I

I

T

T

L

R

G

G

V

V

S

S

K
|
K

Q
|
Q

L

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

T

T

T

P

P

V

E

D

D

L

K

P

G

Q

Y

Y

F

R

Q

N

R

L

L

G

G

D

D

G

G

S

S

Q

E

F

F

G

G

V

I

R

Q

F

L

S

E

Y

Y

A

A

E

E

Q
|
Q

P

P

T

S

P
|
P

D

D

G

G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

E

T

F

F

I

L

G

N

D

G

D

E

P

P

V

S

C

C

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

H

F

G

G

Q

Q

H

G

F

F

G

S

E

P

Q

K

L

L

L

R

G

H

A

V

A

A

K

A

R

R

A

T

S

E

G

G

A

A

T

T

V

V

F

F

G

G

Y

Y

W

Q

V

V

K

M

D

D

P

P

E

E

R

R

F

F

G

G

V

V

I

V

D

E

F

F

D

D

S

D

E

N

G

F

R

R

A

A

L

I

S

S

I

L

E

E

K

K

P

P

A

K

K

Q

P

P

K

K

S

S

S

N

Y

W

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

S

D

K

V

V

I

V

K

E

I

Y

A

A

K

K

A

Q

V

V

K

K

P

P

S

S

P

E

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

S

V

I

N

N

N

Q

A

M

Y

Y

L

L

K

E

R

A

G

G

D

N

L

L

H

T

V

V

E

E

R

L

F

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

L

I

E

E

A

A

S

S

Q

T

Y

F

V

V

Q

Q

T

T

I

V

E

E

H

K

R

R

Q

Q

G

G

L

F

K

K

V

I

A

A

C

C

L

L

E

E

V

I

A

A

Y

W

E

R

N

N

G

G

W

W

I

L

N

D

R

D

E

E

H

G

L

V

L

K

E

R

R

A

A

A

K

S

Y

S

F

L

G

A

K

K

T

T

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

L

S

E

L

L

binding magnesium ion: D109 (= D109), D224 (= D224)
binding thymidine-5'-triphosphate: L7 (= L7), G9 (= G9), G10 (= G10), G12 (= G12), T13 (= T13), R14 (= R14), K24 (= K24), Q25 (= Q25), Q81 (= Q81), P84 (= P84), L87 (= L87), D109 (= D109)

1lvwA Crystal structure of glucose-1-phosphate thymidylyltransferase, rmla, complex with dtdp
67% identity, 99% coverage: 2:290/291 of query aligns to 4:292/295 of 1lvwA

query
sites
1lvwA

M

M

K

K

G

G

I

I

V

V

L
|
L

A

A

G
|
G

G

G

S

S

G

G

T

T

R

R

L

L

H

Y

P

P

I

I

T

T

L

R

G

A

V

V

S

S

K

K

Q
|
Q

L

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

K

R

D

D

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

R

D

D

L

L

P

P

Q

L

Y

Y

R

R

N

D

L

L

G

G

D

D

G

G

S

S

Q

Q

F

F

G

G

V

V

R

R

F

F

S

S

Y

Y

A

R

E

V

Q
|
Q

P

E

T

E

P

P

D

R

G
|
G

L

I

A

A

Q

D

A

A

F

F

I

I

I

V

G

G

E

K

E

D

F

F

I

I

G

G

D

D

D

S

P

K

V

V

C

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

V

F

F

H
x
Y

G
|
G

Q
x
H

H
x
R

F
|
F

G
x
S

E

E

Q

I

L

L

L

R

G

R

A

A

K

S

R

L

A

E

S

D

G

G

A

A

T

V

V

I

F

F

G

G

Y

Y

W

Y

V

V

K

R

D

D

P

P

E

R

R

P

F

F

G

G

V

V

I

V

D

E

F

F

D

D

S

S

E

E

G

G

R

R

A

V

L

I

S

S

I

I

E

E

K

K

P

P

A

S

K

R

P

P

K

K

S

S

S

N

Y

Y

A

V

V

V

T

P

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

D

Q

V

V

I

V

K

E

I

I

A

A

K

R

A

R

V

I

K

E

P

P

S

S

P

D

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

S

V

V

N

N

N

E

A

E

Y

Y

L

L

K

R

R

M

G

G

D

K

L

L

H

R

V

V

E

E

R

L

F

M

G

G

R

R

G

G

F

M

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

G

L

L

E

E

A

A

S

S

Q

S

Y

F

V

I

Q

E

T

T

I

I

E

Q

H

K

R

R

Q

Q

G

G

L

F

K

Y

V
x
I

A

A

C

C

L

L

E

E

V
x
I

A

A

Y

Y

E

N

N

N

G

G

W

W

I

I

N

T

R

R

E

E

H

D

L

V

L

L

E

E

R

M

A

A

K

E

Y

K

F

L

G

E

K

K

T

T

G

D

Y

Y

G

G

Q

K

Y

Y

L

L

Y

R

S

D

L

L

A

A

G

E

E

G

N

N

binding thymidine-5'-diphosphate: L9 (= L7), G11 (= G9), Q27 (= Q25), Q83 (= Q81), G88 (= G86), L109 (= L107), D111 (= D109), Y115 (≠ H113), G116 (= G114), H117 (≠ Q115), R118 (≠ H116), F119 (= F117), S120 (≠ G118), I251 (≠ V249), I257 (≠ V255)

4b5bA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 4:291/293 of 4b5bA

query
sites
4b5bA

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q

Q

P

P

T

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

H

Y

G
|
G

Q

H

H

D

F

F

G

H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

K

K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A
|
A

C

C

L

P

E

E

E
|
E

V
x
I

A

A

Y

Y

E

R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N,3-dimethylbenzenesulfonamide: S41 (= S40), L45 (= L44), Y113 (≠ F112), G115 (= G114), V250 (= V249), A251 (= A250), E255 (= E254), I256 (≠ V255)

4b4gA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 4:291/293 of 4b4gA

query
sites
4b4gA

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q

Q

P

P

T

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

H

Y

G
|
G

Q

H

H

D

F
|
F

G

H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

K

K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A
|
A

C

C

L

P

E

E

E
|
E

V
x
I

A

A

Y

Y

E
x
R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbutane-1-sulfonamide: L45 (= L44), Y113 (≠ F112), G115 (= G114), F118 (= F117), V250 (= V249), A251 (= A250), E255 (= E254), I256 (≠ V255), R259 (≠ E258)

4b42A Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 4:291/293 of 4b42A

query
sites
4b42A

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S
|
S

V
x
T

L

L

M

M

L

L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q

Q

P

P

T

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

H

Y

G

G

Q

H

H

D

F
|
F

G

H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

K

K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A

A

C

C

L

P

E

E

E
|
E

V
x
I

A

A

Y

Y

E

R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding N-[6-azanyl-1-butyl-2,4-bis(oxidanylidene)pyrimidin-5-yl]-N-methyl-benzamide: S41 (= S40), T42 (≠ V41), Y113 (≠ F112), F118 (= F117), V250 (= V249), E255 (= E254), I256 (≠ V255)

4b3uA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 10:297/299 of 4b3uA

query
sites
4b3uA

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q

Q

P

P

T

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F

Y

H

Y

G

G

Q

H

H

D

F

F

G

H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

K

K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V

V

A

A

C

C

L

P

E

E

E
|
E

V
x
I

A

A

Y

Y

E
x
R

N
x
Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding 6-amino-1-benzyl-5-(ethylamino)pyrimidine-2,4(1H,3H)-dione: L51 (= L44), E261 (= E254), I262 (≠ V255), R265 (≠ E258), Q266 (≠ N259)

4asyA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 4:291/293 of 4asyA

query
sites
4asyA

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S
|
S

V
x
T

L

L

M

M

L
|
L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q

Q

P

P

T

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

H

Y

G
|
G

Q

H

H

D

F

F

G

H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

K

K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A

A

C

C

L

P

E

E

E
|
E

V

I

A

A

Y

Y

E

R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzamide: S41 (= S40), T42 (≠ V41), L45 (= L44), Y113 (≠ F112), G115 (= G114), V250 (= V249), E255 (= E254)

4b4mA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 7:294/296 of 4b4mA

query
sites
4b4mA

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S
|
S

V
x
T

L

L

M

M

L
|
L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q

Q

P

P

T

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

H

Y

G
|
G

Q

H

H

D

F

F

G

H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

K

K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A
|
A

C

C

L

P

E

E

E
|
E

V
x
I

A

A

Y

Y

E
x
R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-4-fluoro-N-methylbenzenesulfonamide: S44 (= S40), T45 (≠ V41), L48 (= L44), Y116 (≠ F112), G118 (= G114), V253 (= V249), A254 (= A250), E258 (= E254), I259 (≠ V255), R262 (≠ E258)

4asjA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 3:290/292 of 4asjA

query
sites
4asjA

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q

Q

P

P

T

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

H

Y

G
|
G

Q

H

H

D

F
|
F

G

H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A
|
A

L

I

S

S

I

L

E

E

K

K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V
|
V

E

E

R

I

F

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A

A

C

C

L

P

E

E

E
|
E

V

I

A

A

Y

Y

E

R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide: S40 (= S40), L44 (= L44), Y112 (≠ F112), G114 (= G114), F117 (= F117), V249 (= V249), E254 (= E254)
binding phosphate ion: A155 (= A155), V213 (= V213)

1g3lA The structural basis of the catalytic mechanism and regulation of glucose-1-phosphate thymidylyltransferase (rmla). Tdp-l-rhamnose complex. (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 3:290/292 of 1g3lA

query
sites
1g3lA

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q
|
Q

P

P

T

S

P
|
P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

L

F

Y

H
x
Y

G
|
G

Q
x
H

H
x
D

F
|
F

G
x
H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F
x
Y

G
|
G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

E
|
E

K
|
K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V
|
V

T
|
T

G
|
G

L

L

Y
|
Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R
|
R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G
|
G

R
|
R

G
|
G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V

V

A

A

C

C

L

P

E

E

E
|
E

V
x
I

A

A

Y

Y

E
x
R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding 2'-deoxy-thymidine-beta-l-rhamnose: L7 (= L7), G9 (= G9), G10 (= G10), Q81 (= Q81), P84 (= P84), D109 (= D109), Y113 (≠ H113), G114 (= G114), H115 (≠ Q115), D116 (≠ H116), F117 (= F117), H118 (≠ G118), Y144 (≠ F144), G145 (= G145), E160 (= E160), K161 (= K161), V171 (= V171), T172 (= T172), G173 (= G173), Y175 (= Y175), R193 (= R193), G217 (= G217), R218 (= R218), G219 (= G219), E254 (= E254), I255 (≠ V255), R258 (≠ E258)

1g2vA The structural basis of the catalytic mechanism and regulation of glucose-1-phosphate thymidylyltransferase (rmla). Ttp complex. (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 3:290/292 of 1g2vA

query
sites
1g2vA

K

K

G

G

I

I

V

I

L
|
L

A
|
A

G
|
G

S

S

G
|
G

T
|
T

R
|
R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q
|
Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q
|
Q

P

P

T

S

P
|
P

D

D

G

G

L
|
L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

L

F

Y

H
x
Y

G
|
G

Q
x
H

H
x
D

F
|
F

G
x
H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

K

K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G
|
G

R
|
R

G
|
G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V

V

A

A

C

C

L

P

E

E

E
|
E

V
x
I

A

A

Y

Y

E

R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding thymidine-5'-triphosphate: L7 (= L7), A8 (= A8), G9 (= G9), G10 (= G10), G12 (= G12), T13 (= T13), R14 (= R14), Q25 (= Q25), Q81 (= Q81), P84 (= P84), L87 (= L87), D109 (= D109), Y113 (≠ H113), G114 (= G114), H115 (≠ Q115), D116 (≠ H116), F117 (= F117), H118 (≠ G118), G217 (= G217), R218 (= R218), G219 (= G219), E254 (= E254), I255 (≠ V255)

1g1lA The structural basis of the catalytic mechanism and regulation of glucose-1-phosphate thymidylyltransferase (rmla). Tdp-glucose complex. (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 3:290/292 of 1g1lA

query
sites
1g1lA

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q
|
Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q
|
Q

P

P

T

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

L

F

Y

H
x
Y

G
|
G

Q
x
H

H
x
D

F
|
F

G
x
H

E
|
E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F
x
Y

G
|
G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

E
|
E

K
|
K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G
|
G

R
|
R

G
|
G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A
|
A

C

C

L

P

E

E

E
|
E

V
x
I

A

A

Y

Y

E
x
R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L7 (= L7), G9 (= G9), G10 (= G10), Q25 (= Q25), Q81 (= Q81), P84 (= P84), G86 (= G86), L87 (= L87), L107 (= L107), D109 (= D109), Y113 (≠ H113), G114 (= G114), H115 (≠ Q115), D116 (≠ H116), F117 (= F117), H118 (≠ G118), E119 (= E119), Y144 (≠ F144), G145 (= G145), E160 (= E160), K161 (= K161), V171 (= V171), G217 (= G217), R218 (= R218), G219 (= G219), V249 (= V249), A250 (= A250), E254 (= E254), I255 (≠ V255), R258 (≠ E258)

Sites not aligning to the query:

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: 292

1g0rA The structural basis of the catalytic mechanism and regulation of glucose-1-phosphate thymidylyltransferase (rmla). Thymidine/glucose- 1-phosphate complex. (see paper)
65% identity, 99% coverage: 3:290/291 of query aligns to 3:290/292 of 1g0rA

query
sites
1g0rA

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

L

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

K

R

D

E

I

I

L

L

V

I

I

I

S

S

T

T

P

P

V

Q

D

D

L

T

P

P

Q

R

Y

F

R

Q

N

Q

L

L

G

G

D

D

G

G

S

S

Q

N

F

W

G

G

V

L

R

D

F

L

S

Q

Y

Y

A

A

E

V

Q

Q

P

P

T

S

P

P

D

D

G

G

L
|
L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

P

L

V

S

C

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F

Y

H

Y

G

G

Q

H

H

D

F

F

G

H

E

E

Q

L

L

L

L

G

G

S

A

A

A

S

K

Q

R

R

A

Q

S

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

K

L

D

D

P

P

E

E

R

R

F
x
Y

G
|
G

V

V

I

V

D

E

F

F

D

D

S

Q

E

G

G

G

R

K

A

A

L

I

S

S

I

L

E

E

E
|
E

K
|
K

P

P

A

L

K

E

P

P

K

K

S

S

S

N

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

D

Q

V

V

I

V

K

D

I

I

A

A

K

R

A

D

V

L

K

K

P

P

S

S

P

P

R

R

G

G

E

E

Y

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

R

R

G

G

D

Q

L

L

H

S

V

V

E

E

R

I

F

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

E

E

A

A

S

G

Q

Q

Y

F

V

I

Q

A

T

T

I

L

E

E

H

N

R

R

Q

Q

G

G

L

L

K

K

V

V

A

A

C

C

L

P

E

E

V

I

A

A

Y

Y

E

R

N

Q

G

K

W

W

I

I

N

D

R

A

E

A

H

Q

L

L

L

E

E

K

R

L

A

A

K

A

Y

P

F

L

G

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

Y

K

S

R

L

L

A

L

G

T

E

E

N

T

binding 1-O-phosphono-alpha-D-glucopyranose: L87 (= L87), Y144 (≠ F144), G145 (= G145), E160 (= E160), K161 (= K161), V171 (= V171)

Query Sequence

>GFF260 FitnessBrowser__WCS417:GFF260
MMKGIVLAGGSGTRLHPITLGVSKQLLPVYDKPMIYYPISVLMLAGIKDILVISTPVDLP
QYRNLLGDGSQFGVRFSYAEQPTPDGLAQAFIIGEEFIGDDPVCLILGDNIFHGQHFGEQ
LLGAAKRASGATVFGYWVKDPERFGVIDFDSEGRALSIEEKPAKPKSSYAVTGLYFYDND
VIKIAKAVKPSPRGEYEITDVNNAYLKRGDLHVERFGRGFAWLDTGTHDSLLEASQYVQT
IEHRQGLKVACLEEVAYENGWINREHLLERAKYFGKTGYGQYLYSLAGENQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory