SitesBLAST

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
26% identity, 53% coverage: 47:292/467 of query aligns to 96:317/495 of Q9NRA2

query
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Q9NRA2

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K136 (= K87) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ I152) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AT 167:168) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ IR 234:235) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ SDQTV 236:240) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
328 G → E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
334 P → R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
371 G → V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 35% coverage: 130:291/467 of query aligns to 161:316/495 of Q5Q0U0

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Q5Q0U0

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R168 (= R137) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L140) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (≠ A141) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E144) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A145) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (= F148) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P149) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ I152) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ T155) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ SDQTV 236:240) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
136 K→E: Markedly decreases H(+)-coupled sialic acid transporter activity.
334 P→R: Abolishes H(+)-coupled sialic acid transporter activity.
371 G→V: Remains in the endoplasmic reticulum.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
29% identity, 35% coverage: 156:320/467 of query aligns to 145:309/452 of 7t3nA

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binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y165 (≠ N176), R264 (≠ T276), F268 (≠ W280), Y269 (≠ W281)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E163), G163 (= G174)

Sites not aligning to the query:

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
28% identity, 33% coverage: 137:291/467 of query aligns to 168:316/495 of Q8BN82

query
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Q8BN82

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H183 (≠ I152) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
28% identity, 39% coverage: 137:320/467 of query aligns to 184:367/582 of Q9JI12

query
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Q9JI12

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R184 (= R137) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E144) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ T276) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
128 H→A: Greatly lowers L-glutamate transport.

Q6ZSM3 Monocarboxylate transporter 12; MCT 12; Creatine transporter 2; CRT2; Solute carrier family 16 member 12 from Homo sapiens (Human) (see 3 papers)
23% identity, 66% coverage: 17:323/467 of query aligns to 45:339/516 of Q6ZSM3

query
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Q6ZSM3

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G

-

A

-

I

-

G

-

L

-

A

-

V

-

I

-

P

-

V

-

S

S

I

L

A

K

G

H

F

L

M

Y

L

L

S

T

R

L

A

G

V

V

L

L

A

T

-

G

I

L

G

G

E

F

A

A

G

L

N

C

F

Y

P

S

I

P

A

A

I

I

K

A

A

M

T

V

A

G

E

K

Y

Y

F

F

P

S

K

R

E

-

R

K

S

A

L

L

A

A

T

Y

G

G

I

I

F

A

N

M

S

S

G

G

A

S

N

G

V

I

G

G

A

T

-

F

I

I

L

L

A

A

P

P

I

V

C

-

V

V

P

Q

L

L

I

L

A

I

G

E

L

Q

W

F

G

S

W

W

E

R

A

G

A

A

F

L

I

L

V

I

I

L

G

G

L

F

G

V

F

L

V

N

W

L

V

C

V

V

V

C

W

G

I

A

A

L

L

M

Y

R

Q

P

K

I

P

T

E

L

E

K

Q

E

K

D

R

H

L

T

S

T

P

P

E

E

E

Q

L

N

A

H

Y

V

I

C

R

R

S

T

D
x
Q

-
x
K

Q
x
E

T
x
D

V
x
I

Q
x
K

P
x
R

F
x
V

T
x
S

P
|
P

A
x
Y

P
x
S

A
x
S

G
x
L

A
x
T

P
x
K

E
|
E

K
x
W

K
x
A

V
x
Q

S
x
T

W
x
C

F
x
L

K

-

L
x
C

T
x
C

Y
x
L

R
x
Q

Q
|
Q

T
x
E

W
x
Y

A
x
S

F
|
F

A
x
L

F
x
L

G
x
M

K
x
S

-
x
D

-
x
F

-
x
V

-
x
L

-
x
A

-
x
V

-
x
S

-
x
V

-
x
L

F
|
F

M
|
M

T
x
A

D
x
Y

G
|
G

V
x
C

W
x
S

W
x
P

F
x
L

F
|
F

L
x
V

F
x
Y

W
x
L

L
x
V

P
|
P

T
x
Y

Y
x
A

L
|
L

S
|
S

A
x
V

Q
x
G

Y
x
V

G
x
S

M
x
H

K
x
Q

G
x
Q

A
|
A

D
x
A

I
x
F

V
x
L

M
|
M

P

-

L

-

A
x
S

V
x
I

L
|
L

Y
x
G

S
x
V

M
x
I

T
x
D

M
x
I

V
x
I

G
|
G

S
x
N

I
|
I

G
x
T

G
x
F

G
|
G

W
|
W

F
x
L

R67 (≠ Q39) mutation to A: Abolishes creatine efflux activity. Does not affect plasma membrane localization.
D95 (≠ Y70) mutation to A: Decreases in the creatine efflux activity. Does not affect plasma membrane localization.
D329 (≠ T313) mutation to A: Decreases creatine efflux activity. Loss of localization to the plasma membrane.

Sites not aligning to the query:

245:516 natural variant: Missing (in CTRCT47; loss of localization to the plasma membrane; retained in the endoplasmic reticulum where it undergoes degradation by the proteasome; has no dominant effect on wild-type protein expression and localization)
360 D→A: Does not affect creatine efflux activity. Does not affect plasma membrane localization.
387 D→A: Does not affect creatine efflux activity.
437 G → S: found in a patient with age-related cataract; uncertain significance; decreases creatine transport; dbSNP:rs759863805

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
23% identity, 82% coverage: 37:420/467 of query aligns to 57:397/444 of Q8NLB7

query
sites
Q8NLB7

D
|
D

R

L

Q

I

V

V

L

Y

S

G

L

T

L

V

A

Q

P

S

Q

A

L

L

S

A

T

K

Q

E

F

W

G

N

W

L

S

S

N

S

T

A

D

T

Y

L

A

G

N

T

I

I

A

G

A

S

V

T

F

A

Q

F

F

F

V

G

Y

M

A

A

I

I

S

G

M

A

L

V

F

F

A

I

G

G

R

R

F

L

V

S

D

D

K
x
R

I

V

G

G

T

R

K

K

A

A

Y

V

V

I

V

G

A

S

I

V

A

L

I

I

W

L

S

S

T

V

G

F

A

T

I

M

M

L

H

C

A

A

F

F

S

-

V

-

P

-

M

-

G

-

E

-

G

-

I

-

A

-

I

-

S

-

G

-

A

-

I

-

G

-

L

-

A

A

V

P

I

N

P

P

V

W

S

V

I

F

A

G

G

A

F

F

M

-

L

-

S

-

R

R

A

F

V

I

L

A

A

G

I

L

G

G

E

L

A

G

G

G

N

L

F

V

P

P

I

S

A

V

I

N

K

A

A

M

T

T

A

S

E

D

Y

L

F

V

P

P

K

R

K

K

E

T

R

M

S

S

L

A

A

W

T

A

G

T

I

V

F

M

N

M

S

S

G

G

A

V

N

P

V

I

G

G

-

G

-

S

-

I

-

A

A

A

I

V

L

L

A

A

P

L

I

V

C

V

V

V

P

P

L

-

I

S

A

S

G

E

L

E

W

W

G

G

W

W

E

R

A

-

F

-

I

-

V

-

I

-

G

-

L

-

G

-

F

F

V

M

W

F

V

L

V

I

V

A

W

L

I

I

A

P

L

L

Y

V

Q

V

K

G

P

L

E

P

E

I

Q

A

K

M

R

K

L

V

S

I

P

P

E

S

E

D

L

K

A

A

Y

-

I

I

R

K

S

A

D

D

Q

H

T

D

V

I

Q

R

P

E

F

G

T

H

P

D

A

E

P

P

A

A

G

G

A

F

P

K

E

D

K

-

V

-

S

-

W

-

F

-

K

-

L

L

T

V

Y

D

R

R

Q

Y

T

R

W

W

A

I

-

S

-

I

-

W

F

F

A

A

F

L

G

A

K

T

F

F

M

V

T

T

D

L

G

L

V

A

W

W

W

Y

F

G

F

L

L

G

F

T

W

W

L

L

P

P

T

R

Y

L

L

M

S

E

A

T

Q

A

Y

G

G

Y

M

E

K

F

G

G

A

H

D

A

I

L

V

M

M

F

P

T

L

L

A

A

V

-

L

L

Y

N

S

L

M

G

T

A

M

V

V

I

G

G

S

S

I

V

G

V

G

T

G

A

W
|
W

F

A

P

G

S
x
D

Y
x
R

F

F

-

G

-

P

M
x
I

A

R

R

S

G

G

D

V

A

I

P

A

Y

A

D

G

G

I

R

A

M

G

K

I

A

A

M

L

L

L

V

L

I

L

A

L

L

T

F

Y

P

P

L

P

V

V

V

T

L

-

A

-

Q

-

P

-

L

-

G

-

Y

-

I

-

S

A

F

V

W

Y

V

V

P

I

V

L

L

I

L

L

I

A

G

G

V

V

G

G

A

T

S

H

A

G

H

T

Q

Q

A

-

W

-

S

-

C

I

N

L

I

I

F

I

T

A

V

V

S

A

D

N

M

F

F

Y

P

P

Q

S

K

N

T

L

V

R

A

G

S

T

V

A

V

L

G

G

I

W

G

A

G

L

M

G

A

V

G

G

G
x
R

L

I

G

G

G

A

V

V

M

A

T

P

K

Q

I

L

G

A

G

G

D57 (= D37) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (≠ K83) mutation to A: Loss of transport activity.
W309 (= W322) mutation to V: Loss of transport activity.
D312 (≠ S325) mutation to A: Loss of transport activity.
R313 (≠ Y326) mutation to A: Loss of transport activity.
I317 (≠ M328) mutation I->H,Y: Loss of transport activity.
R386 (≠ G409) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
21% identity, 52% coverage: 14:254/467 of query aligns to 21:245/448 of Q51955

query
sites
Q51955

Q

Q

A

P

V

L

G

S

N

R

Y

Y

R

Q

W

W

T

R

I

V

C

V

A

L

M

L

L

C

F

F

F

L

A

I

T

V

T

F

V

L

N
x
D

Y

G

L

L

D
|
D

R

T

Q

A

V

A

L

M

S

G

L

F

L

I

A

A

P

P

Q

A

L

L

S

S

T

Q

Q

E

F

W

G

G

W

I

S

D

N

R

T

A

D

S

Y

L

A

G

N

P

I

V

A

M

A

S

V

A

F

A

Q

L

F

I

V

G

Y

M

A

V

I

F

S

G

M

A

L

L

F

G

A

S

G
|
G

R

P

F

L

V

A

D
|
D

K

R

I

F

G
|
G

T

R

K

K

A

G

A

V

Y

L

V

V

V

G

A

A

I

V

A

L

I

V

W

F

S

G

T

G

G

F

A

S

I

L

M

A

H

S

A

A

F

Y

S

A

V

T

P

N

M

V

G

D

E

Q

G

-

I

-

A

-

I

-

S

-

G

-

A

-

I

-

G

-

L

-

A

-

V

-

I

-

P

-

V

-

S

-

I

-

A

-

G

-

F

L

M

L

L

V

S

L

R
|
R

A

F

V

L

L

T

A

G

I

L

G

G

E

L

A

G

G

A

N

G

F

M

P

P

I

N

A

A

I

T

K

T

A

L

T

L

A

S

E
|
E

Y

Y

F

T

P

P

K

E

K

R

E

L

R

K

S

S

-

L

L

L

A

V

T

T

G

S

I

M

-

F

-

C

-

G

F

F

N

N

S

L

G

G

A

M

N

A

V

G

G

G

A

G

I

F

L

I

A

S

P

A

I

K

C

M

V

I

P

P

L

-

I

-

A

-

G

-

L

A

W

Y

G

G

W

W

E
x
H

A

-

A

S

F

L

I

L

V

V

I

I

G

G

L

V

G

-

F

L

V

P

W

L

V

L

V

A

W

L

I

V

A

L

L

M

Y

V

Q

W

K

L

P

P

E

E

E

S

Q

A

K

R

R

F

L

L

S

V

P

V

E

R

E

N

L

R

A

G

Y

T

I

D

R

K

S

I

D

R

Q

K

T

T

V

L

Q

S

P

P

F

I

T

A

P

P

-

Q

-

V

A

A

P

E

A

A

G

G

A

S

-

F

-

S

-

V

P

P

E

E

K

Q

K

K

D41 (≠ N34) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D37) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (= G78) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (= D82) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G85) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R137) mutation to A: Abolishes 4-HBA transport.
E144 (= E157) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ E196) mutation to A: Decrease in 4-HBA transport and chemotaxis.

Sites not aligning to the query:

323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
24% identity, 42% coverage: 160:357/467 of query aligns to 193:386/497 of Q9Y2C5

query
sites
Q9Y2C5

P

P

K

P

K

L

E

E

R

R

S

S

L

Q

A

L

T

T

G

T

I

I

F

A

N

G

S

S

G

G

A

S

N

M

V

L

G

G

A

S

I

F

L

I

A

V

P

L

I

L

C

A

V

G

P

G

L

L

I

L

A

C

G

Q

L

T

W

I

G

G

W

W

E

P

A

Y

A

V

F

F

I

Y

V

I

I

F

G

G

L

I

G

G

F

C

V

A

W

C

V

C

V

P

V

L

W

W

I

F

A

P

L

L

-

I

Y

Y

Q

D

K

D

P

P

E

V

E

N

Q

H

K

P

R

F

L

I

S

S

P

A

E

G

E

E

L

K

A

R

Y

Y

I

I

R

V

S

C

D

S

Q

L

T

A

V

Q

Q

Q

P

D

F

C

T

S

P

P

A

G

P

W

A

S

G

L

A

P

P

I

E

R

K

A

K

M

V

I

S

K

W

S

F

L

K

P

L

L

L

-

T

-

Y

-

R

-

Q

-

T

-

W

W

A

A

F

I

A

L

F

V

G

S

K

Y

F

F

M

C

T

E

D

Y

G

W

V

L

W

F

W

Y

F

T

F

I

L

M

F

A

W

Y

L

T

P

P

T

T

Y

Y

L

I

S

S

A

S

-

V

-

L

Q

Q

Y

A

G

N

M

L

K

R

G

D

A

S

D

G

I

I

V

L

M

S

P

A

L

L

A

P

V

F

L

V

Y

V

S

G

M

C

T

I

M

C

V

I

G

-

S

-

I

I

G

L

G

G

W

L

F

L

P

A

S

D

Y

F

F

L

M

L

A

S

R

R

G

K

D

I

A

L

P

R

Y

L

D

I

G

T

R

I

M

R

K

K

A

L

M

F

L

T

V
x
A

I

I

A

G

-

V

L

L

F

F

P

P

L

S

V

V

V

I

L

L

L

V

A

S

Q

L

P

P

A372 (≠ V344) to T: in dbSNP:rs11754288

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
20% identity, 51% coverage: 1:236/467 of query aligns to 5:217/452 of Q5EXK5

query
sites
Q5EXK5

M

L

N

E

L

L

N

Q

E

K

P

L

I

L

N

N

A

A

H

-

R

-

V

-

G

-

Q

A

A

P

V

V

G

G

N

A

Y

R

R

Q

W

W

T

R

I

V

C

I

A

I

M

C

L

C

F

F

F

L

A

V

T

V

T

M

V

L

N

D

Y

G

L

F

D

D

R

T

Q

A

V

A

L

I

S

G

L

F

L

I

A

A

P

P

Q

D

L

I

S

R

T

S

Q

H

F

W

G

Q

W

L

S

T

N

A

T

G

D

D

Y

L

A

A

N

P

I

L

A

F

A

G

V

A

F

G

Q

L

F

L

V

G

Y

L

A

T

I

A

S

G

M

A

L

L

F

L

A

C

G

G

R

P

F

L

V

S

D
|
D

K

R

I

F

G

G

T

R

K

K

A

R

A

V

Y

I

V

E

V

F

A

C

I

V

A

A

I

L

W

F

S

G

T

L

G

F

A

S

I

L

M

L

H

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A

A

F

F

S

A

V

P

P

-

M

-

G

-

E

-

G

-

I

-

A

-

I

-

S

-

G

-

A

-

I

-

G

-

L

-

A

-

V

-

I

-

P

-

V

-

S

D

I

L

A

Q

G

M

F

L

M

V

L

F

S

L

R

R

A

F

V

L

L

T

A

G

I

L

G

G

E

L

A

G

G

G

N

A

F

M

P

P

I

N

A

T

I

I

K

T

A

M

T

T

A

S

E

E

Y

Y

F

L

P

P

K

A

K

R

E

R

R

R

-

G

-

A

-

L

-

V

S

T

L

L

A

M

T

F

G

C

I

G

F

F

N

T

S

L

G

G

A

S

N

A

V

F

G

G

A

G

I

V

L

V

A

S

P

A

I

Q

C

L

V

V

P

P

L

L

I

I

A

-

G

-

L

-

W

-

G

G

W

W

E

H

A

G

A

I

F

L

I

V

V

L

I

G

G

G

G

V

L

L

G

P

F

L

V

I

W

L

V

A

V

V

V

A

W

L

I

V

A

P

L

L

Y

L

-

P

Q

E

K

S

P

P

E

R

E

W

Q

Q

K

I

R

R

L

R

S

Q

P

L

E

P

E

Q

L

A

A

T

Y

I

I

A

R

R

S

T

D82 (= D82) mutation to A: Loss of activity.

Sites not aligning to the query:

311 V→W: Loss of activity.
314 D→A: Loss of activity.

Query Sequence

>GFF2889 FitnessBrowser__WCS417:GFF2889
MNLNEPINAHRVGQAVGNYRWTICAMLFFATTVNYLDRQVLSLLAPQLSTQFGWSNTDYA
NIAAVFQFVYAISMLFAGRFVDKIGTKAAYVVAIAIWSTGAIMHAFSVPMGEGIAAISGA
IGLAVIPVSIAGFMLSRAVLAIGEAGNFPIAIKATAEYFPKKERSLATGIFNSGANVGAI
LAPICVPLIAGLWGWEAAFIVIGGLGFVWVVVWIALYQKPEEQKRLSPEELAYIRSDQTV
QPFTPAPAGAPEKKVSWFKLLTYRQTWAFAFGKFMTDGVWWFFLFWLPTYLSAQYGMKGA
DIVMPLAVLYSMTMVGSIGGGWFPSYFMARGDAPYDGRMKAMLVIALFPLVVLLAQPLGY
ISFWVPVLLIGVGASAHQAWSCNIFTTVSDMFPQKTVASVVGIGGMAGGLGGVVMTKIGG
WVFDYYKSVGDIHTGYMIMFAICALAYLVAWSVMKTLVPRHKEITDL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory