SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF325 FitnessBrowser__WCS417:GFF325 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A9C5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Escherichia coli (strain K12) (see 3 papers)
69% identity, 99% coverage: 5:468/468 of query aligns to 7:469/469 of P0A9C5

query
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Y398 (= Y397) modified: O-AMP-tyrosine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0A1P6 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
68% identity, 99% coverage: 5:468/468 of query aligns to 7:469/469 of P0A1P6

query
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E130 (= E129) binding Mn(2+)
E132 (= E131) binding Mn(2+)
E208 (= E207) binding ATP
E213 (= E212) binding Mn(2+)
E221 (= E220) binding Mn(2+)
NG 265:266 (= NG 264:265) binding L-glutamate
H270 (= H269) binding Mn(2+)
HMS 272:274 (≠ HLS 271:273) binding ATP
R322 (= R321) binding L-glutamate
E358 (= E357) binding Mn(2+)
R360 (= R359) binding L-glutamate

1fpyA Crystal structure of glutamine synthetase from salmonella typhimurium with inhibitor phosphinothricin (see paper)
68% identity, 99% coverage: 5:468/468 of query aligns to 6:468/468 of 1fpyA

query
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1fpyA

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A

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

S

A

S

S

P

P

R

K

A

A

R

R

R
|
R

I

I

E
|
E

A

V

R
|
R

F

F

P

P

D

D

P

P

A

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

L

L

V

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

D

E

A

P

A

M

D

D

K

K

N

N

L

L

Y

Y

D

D

L

L

P

P

P

P

E

E

E

E

A

A

K

K

E

E

I

I

P

P

Q

Q

V

V

C

A

G

G

S

S

L

L

K

E

E

E

A

A

L

L

E

N

E

A

L

L

D

D

K

L

G

D

R

R

A

E

F

F

L

L

T

K

K

A

G

G

G

G

V

V

F

F

S

T

D

D

D

E

F

A

I

I

D

D

A

A

Y

Y

I

I

G

A

L

L

K

R

S

R

E

E

E

E

E

D

I

D

K

R

V

V

R

R

T

M

F

T

V

P

H

H

P

P

L

V

E

E

Y

F

E

E

L

L

Y

Y

Y

Y

S

S

C

V

binding adenosine-5'-diphosphate: G127 (= G127), E129 (= E129), E207 (= E207), T223 (≠ V223), F225 (= F225), H271 (= H271), S273 (= S273), R355 (= R355), E357 (= E357)
binding manganese (ii) ion: E129 (= E129), E131 (= E131), E212 (= E212), E220 (= E220), H269 (= H269), E357 (= E357)
binding phosphinothricin: E131 (= E131), E212 (= E212), G265 (= G265), H269 (= H269), R321 (= R321), E327 (= E327), R359 (= R359)

1f1hA Crystal structure of glutamine synthetase from salmonella typhimurium with thallium ions (see paper)
68% identity, 99% coverage: 5:468/468 of query aligns to 6:468/468 of 1f1hA

query
sites
1f1hA

V

L

Q

T

L

M

I

L

K

N

D

E

H

H

D

E

V

V

K

K

W

F

I

V

D

D

L

L

R

R

F

F

T

T

D

D

T

T

K

K

G

G

T

K

Q

E

H

Q

H

H

V

V

T

T

M

I

P

P

A

A

R

H

D

Q

A

-

L

V

D

N

E

A

A

E

F

F

F

F

E

E

E

E

G

G

K

K

M

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

K

G

G

I

I

E

N

A

E

S

S

D

D

M

M

I

V

L

L

M

M

P

P

D

D

D

A

S

S

T

T

A

A

V

V

L

I

D

D

P

P

F

F

T

F

E

A

E

D

P

S

T

T

L

L

I

I

L

I

V

R

C

C

D

D

V

I

I

L

E

E

P

P

S

G

T

T

M

L

Q

Q

G

G

Y

Y

D

D

R

R

D

D

P

P

R

R

A

S

I

I

A

A

K

K

R

R

A

A

E

E

E

D

Y

Y

L

L

K

R

S

A

T

T

G

G

I

I

G

A

D

D

T

T

V

V

F

L

V

F

G

G

P

P

E
|
E

P

P

E
|
E

F

F

F

F

I

L

F

F

D

D

Q

D

V

I

K

R

F

F

K

G

S

A

D

S

I

I

S

S

G

G

S

S

M

H

F

V

K

A

I

I

Y

D

S

D

E

I

Q

E

G

G

S

A

W

W

M

N

S

S

D

S

Q

T

D

K

V

Y

E

E

G

G

G

G

N

N

H

K

G

G

H

H

R

R

P

P

G

G

I

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

P

P

P

F

V

D

D

H

S

D

A

H

Q

E

D

I

I

R

R

T

S

S

E

M

M

C

C

N

L

A

V

M

M

E

E

D

Q

M

M

G

G

L

L

T

V

I

V

E
|
E

V

A

H

H

H
|
H

H

H

E
|
E

V

V

A

A

T

T

A

A

G

G

Q

Q

N

N

E
|
E

I

V

G

A

V
x
T

K

R

F
|
F

N

N

T

T

L

M

V

T

A

K

K

K

A

A

D

D

E

E

V

I

Q

Q

T

I

L

Y

K

K

Y

Y

C

V

V

V

H

H

N

N

T

V

A

A

V

H

A

R

Y

F

G

G

R

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

M

Y

F

G

G

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

C

H
|
H

L

M

S
|
S

I

L

A

A

K

K

E

N

G

G

K

T

N

N

T

L

F

F

A

S

G

G

E

D

G

K

Y

Y

A

A

G

G

L

L

S

S

D

E

T

Q

A

A

L

L

Y

Y

F

Y

I

I

G

G

G

G

I

V

I

I

K

K

H

H

G

A

K

K

A

A

L

I

N

N

G

A

F

L

T

A

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

Y

E

E

A

A

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

S

A

S

S

P

P

R

K

A

A

R

R

R
|
R

I

I

E
|
E

A

V

R

R

F

F

P

P

D

D

P

P

A

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

L

L

V

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

D

E

A

P

A

M

D

D

K

K

N

N

L

L

Y

Y

D

D

L

L

P

P

P

P

E

E

E

E

A

A

K

K

E

E

I

I

P

P

Q

Q

V

V

C

A

G

G

S

S

L

L

K

E

E

E

A

A

L

L

E

N

E

A

L

L

D

D

K

L

G

D

R

R

A

E

F

F

L

L

T

K

K

A

G

G

G

G

V

V

F

F

S

T

D

D

D

E

F

A

I

I

D

D

A

A

Y

Y

I

I

G

A

L

L

K

R

S

R

E

E

E

E

E

D

I

D

K

R

V

V

R

R

T

M

F

T

V

P

H

H

P

P

L

V

E

E

Y

F

E

E

L

L

Y

Y

Y

Y

S

S

C

V

binding adenosine-5'-diphosphate: E129 (= E129), E207 (= E207), H210 (= H210), E220 (= E220), T223 (≠ V223), F225 (= F225), H271 (= H271), S273 (= S273), R355 (= R355)
binding manganese (ii) ion: E129 (= E129), E131 (= E131), E212 (= E212), E220 (= E220), H269 (= H269), E357 (= E357)

2lgsA Feedback inhibition of fully unadenylylated glutamine synthetase from salmonella typhimurium by glycine, alanine, and serine (see paper)
63% identity, 99% coverage: 5:468/468 of query aligns to 6:445/445 of 2lgsA

query
sites
2lgsA

V

L

Q

T

L

M

I

L

K

N

D

E

H

H

D

E

V

V

K

K

W

F

I

V

D

D

L

L

R

R

F

F

T

T

D

D

T

T

K

K

G

G

T

K

Q

E

H

Q

H

H

V

V

T

T

M

I

P

P

A

A

R

H

D

Q

A

-

L

V

D

N

E

A

A

E

F

F

F

F

E

E

E

E

G

G

K

K

M

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

-

G

-

I

-

E

-

A

-

S

-

D

D

M

M

I

V

L

L

M

M

P

P

D

D

D

A

S

S

T

T

A

A

V

V

L

I

D

D

P

P

F

F

T

F

E

A

E

D

P

S

T

T

L

L

I

I

L

I

V

R

C

C

D

D

V

I

I

L

E

E

P

P

S

G

T

T

M

L

Q

Q

G

G

Y

Y

D

D

R

R

D

D

P

P

R

R

A

S

I

I

A

A

K

K

R

R

A

A

E

E

E

D

Y

Y

L

L

K

R

S

A

T

T

G

G

I

I

G

A

D

D

T

T

V

V

F

L

V

F

G

G

P

P

E
|
E

P

P

E
|
E

F

F

F

F

I

L

F

F

D

D

Q

D

V

I

K

R

F

F

K

G

S

A

D

S

I

I

S

S

G

G

S

S

M

H

F

V

K

A

I

I

Y

D

S

D

E

I

Q

E

G

G

S

A

W

W

M

N

S

S

D

S

Q

T

D

K

V

Y

E

E

G

G

G

G

N

N

H

K

G

G

H

H

R

R

P

P

G

G

I

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

P

P

P

F

V

D

D

H

S

D

A

H

Q

E

D

I

I

R

R

T

S

S

E

M

M

C

C

N

L

A

V

M

M

E

E

D

Q

M

M

G

G

L

L

T

V

I

V

E

E

V

A

H

H

H

H

H

H

E
|
E

V

V

A

A

T

T

A

A

G

G

Q

Q

N

N

E
|
E

I

V

G

A

V

T

K

R

F

F

N

N

T

T

L

M

V

T

A

K

K

K

A

A

D

D

E

E

V

I

Q

Q

T

I

L

Y

K

K

Y

Y

C

V

V

V

H

H

N

N

T

V

A

A

V

H

A

R

Y

F

G

G

R

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

M

Y

F

G

G

D

D

N
|
N

G
|
G

S

S

G
|
G

M

M

H
|
H

V

C

H

H

L

M

S

S

I

L

A

A

K

K

E

N

G

G

K

T

N

N

T

L

F

F

A

S

G

G

E

D

G

K

Y

Y

A

A

G

G

L

L

S

S

D

E

T

Q

A

A

L

L

Y

Y

F

Y

I

I

G

G

G

G

I

V

I

I

K

K

H

H

G

A

K

K

A

A

L

I

N

N

G

A

F

L

T

A

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

-

F

-

E

-

A

-

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

S

A

S

S

P

P

R

K

A

A

R

R

R

R

I

I

E
|
E

A

V

R
|
R

F

F

P

P

D

D

P

P

A

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

L

L

V

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

D

E

A

P

A

M

D

D

K

-

N

-

L

-

Y

-

D

-

L

-

P

-

P

-

E

-

E

-

A

-

K

-

E

-

I

I

P

P

Q

Q

V

V

C

A

G

G

S

S

L

L

K

E

E

E

A

A

L

L

E

N

E

A

L

L

D

D

K

L

G

D

R

R

A

E

F

F

L

L

T

K

K

A

G

G

G

G

V

V

F

F

S

T

D

D

D

E

F

A

I

I

D

D

A

A

Y

Y

I

I

G

A

L

L

K

R

S

R

E

E

E

E

E

D

I

D

K

R

V

V

R

R

T

M

F

T

V

P

H

H

P

P

L

V

E

E

Y

F

E

E

L

L

Y

Y

Y

Y

S

S

C

V

binding glutamic acid: E125 (= E131), N258 (= N264), G259 (= G265), G261 (= G267), H263 (= H269), R315 (= R321), R349 (= R359)
binding manganese (ii) ion: E123 (= E129), E125 (= E131), E206 (= E212), E214 (= E220), H263 (= H269), E347 (= E357)

1lgrA Interactions of nucleotides with fully unadenylylated glutamine synthetase from salmonella typhimurium (see paper)
63% identity, 99% coverage: 5:468/468 of query aligns to 6:445/445 of 1lgrA

query
sites
1lgrA

V

L

Q

T

L

M

I

L

K

N

D

E

H

H

D

E

V

V

K

K

W

F

I

V

D

D

L

L

R

R

F

F

T

T

D

D

T

T

K

K

G

G

T

K

Q

E

H

Q

H

H

V

V

T

T

M

I

P

P

A

A

R

H

D

Q

A

-

L

V

D

N

E

A

A

E

F

F

F

F

E

E

E

E

G

G

K

K

M

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

-

G

-

I

-

E

-

A

-

S

-

D

D

M

M

I

V

L

L

M

M

P

P

D

D

D

A

S

S

T

T

A

A

V

V

L

I

D

D

P

P

F

F

T

F

E

A

E

D

P

S

T

T

L

L

I

I

L

I

V

R

C

C

D

D

V

I

I

L

E

E

P

P

S

G

T

T

M

L

Q

Q

G

G

Y

Y

D

D

R

R

D

D

P

P

R

R

A

S

I

I

A

A

K

K

R

R

A

A

E

E

E

D

Y

Y

L

L

K

R

S

A

T

T

G

G

I

I

G

A

D

D

T

T

V

V

F

L

V

F

G

G

P

P

E
|
E

P

P

E
|
E

F

F

F

F

I

L

F

F

D

D

Q

D

V

I

K

R

F

F

K

G

S

A

D

S

I

I

S

S

G

G

S

S

M

H

F

V

K

A

I

I

Y

D

S

D

E

I

Q

E

G

G

S

A

W

W

M

N

S

S

D

S

Q

T

D

K

V

Y

E

E

G

G

G

G

N

N

H

K

G

G

H

H

R

R

P

P

G

G

I

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

P

P

P

F

V

D

D

H

S

D

A

H

Q

E

D

I

I

R

R

T

S

S

E

M

M

C

C

N

L

A

V

M

M

E

E

D

Q

M

M

G

G

L

L

T

V

I

V

E
|
E

V

A

H

H

H

H

H

H

E
|
E

V

V

A

A

T

T

A

A

G

G

Q

Q

N

N

E
|
E

I

V

G

A

V
x
T

K

R

F
|
F

N

N

T

T

L

M

V

T

A

K

K

K

A

A

D

D

E

E

V

I

Q

Q

T

I

L

Y

K

K

Y

Y

C

V

V

V

H

H

N

N

T

V

A

A

V

H

A

R

Y

F

G

G

R

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

M

Y

F

G

G

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

C

H
|
H

L

M

S
|
S

I

L

A

A

K

K

E

N

G

G

K

T

N

N

T

L

F

F

A

S

G

G

E

D

G

K

Y

Y

A

A

G

G

L

L

S

S

D

E

T

Q

A

A

L

L

Y

Y

F

Y

I

I

G

G

G

G

I

V

I

I

K

K

H

H

G

A

K

K

A

A

L

I

N

N

G

A

F

L

T

A

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

-

F

-

E

-

A

-

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

S

A

S

S

P

P

R

K

A

A

R

R

R
|
R

I

I

E
|
E

A

V

R

R

F

F

P

P

D

D

P

P

A

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

L

L

V

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

D

E

A

P

A

M

D

D

K

-

N

-

L

-

Y

-

D

-

L

-

P

-

P

-

E

-

E

-

A

-

K

-

E

-

I

I

P

P

Q

Q

V

V

C

A

G

G

S

S

L

L

K

E

E

E

A

A

L

L

E

N

E

A

L

L

D

D

K

L

G

D

R

R

A

E

F

F

L

L

T

K

K

A

G

G

G

G

V

V

F

F

S

T

D

D

D

E

F

A

I

I

D

D

A

A

Y

Y

I

I

G

A

L

L

K

R

S

R

E

E

E

E

E

D

I

D

K

R

V

V

R

R

T

M

F

T

V

P

H

H

P

P

L

V

E

E

Y

F

E

E

L

L

Y

Y

Y

Y

S

S

C

V

binding adenosine monophosphate: E201 (= E207), T217 (≠ V223), F219 (= F225), H265 (= H271), S267 (= S273), R345 (= R355)
binding manganese (ii) ion: E123 (= E129), E125 (= E131), E206 (= E212), E214 (= E220), H263 (= H269), E347 (= E357)

P77961 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Synechocystis sp. (strain PCC 6803 / Kazusa)
53% identity, 99% coverage: 5:468/468 of query aligns to 9:473/473 of P77961

query
sites
P77961

V

L

Q

K

L

W

I

I

K

Q

D

D

H

E

D

N

V

I

K

K

W

I

I

I

D

D

L

L

R

K

F

F

T

I

D

D

T

T

K

P

G

G

T

I

Q

W

H

Q

H

H

V

C

T

S

M

F

P

-

A

Y

R

Y

D

D

A

Q

L

L

D

D

E

E

A

N

F

S

F

F

E

T

E

E

G

G

K

I

M

P

F

F

D

D

G

G

S

S

S

S

I

I

A

R

G

G

W

W

K

K

G

A

I

I

E

N

A

E

S

S

D

D

M

M

I

C

L

M

M

V

P

P

D

D

D

P

S

N

T

T

A

A

V

T

L

I

D

D

P

P

F

F

T

C

E

K

E

E

P

P

T

T

L

L

I

S

L

M

V

I

C

C

D

S

V

I

I

K

E

E

P

P

S

R

T

T

M

G

Q

E

G

W

Y

Y

D

N

R

R

D

D

P

P

R

R

A

T

I

I

A

A

K

A

R

K

A

A

E

V

E

E

Y

Y

L

L

K

R

S

G

T

T

G

G

I

I

G

A

D

D

T

T

V

V

F

Y

V

F

G

G

P

P

E

E

P

A

E
|
E

F

F

F

F

I

L

F

F

D

D

Q

D

V

I

K

R

F

F

K

G

S

Q

D

T

I

E

S

N

G

S

S

S

M

Y

F

Y

K

F

I

A

Y

D

S

S

E

V

Q

E

G

G

S

R

W

W

M

N

S

T

D

G

Q

R

D

E

V

E

E

E

G

G

G

G

N

N

H

L

G

G

H

Y

R

K

P

P

G

G

I

Y

K

K

G

Q

G

G

Y

Y

F

F

P

P

V

V

P

A

P

P

F

T

D

D

H

T

D

A

H

Q

E

D

I

I

R

R

T

T

S

E

M

M

C

L

N

L

A

T

M

M

E

A

D

G

M

L

G

C

L

V

T

P

I

I

E

E

V

K

H

H

H

H

H

H

E
|
E

V

V

A

A

T

S

A

G

G

G

Q

Q

N

N

E
|
E

I

L

G

G

V

I

K

K

F

F

N

D

T

K

L

L

V

V

A

N

K

S

A

A

D

D

E

N

V

L

Q

M

T

I

L

Y

K

K

Y

Y

C

V

V

I

H

K

N

N

T

V

A

A

V

K

A

K

Y

Y

G

G

R

K

T

T

A

V

T

T

F

F

M

M

P

P

K

K

P

P

L

I

Y

F

G

N

D

D

N

N

G

G

S

S

G

G

M

M

H

H

V

V

H

H

L

Q

S

S

I

L

A

W

K

K

E

D

G

G

K

Q

N

P

T

L

F

F

A

A

G

G

E

D

G

K

Y

Y

A

A

G

G

L

F

S

S

D

Q

T

M

A

G

L

L

Y

W

F

Y

I

I

G

G

G

G

I

I

I

L

K

K

H

H

G

A

K

P

A

A

L

L

N

L

G

A

F

F

T

T

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

F

E

E

A

A

P

P

V

V

M

N

L

L

A

A

Y

Y

S

S

A

Q

R

G

N

N

R

R

S

S

A

A

S

S

I

V

R

R

I

I

P

P

Y

L

V

S

-

G

S

G

S

N

P

P

R

K

A

A

R

K

R

R

I

L

E
|
E

A

F

R

R

F

C

P

P

D

D

P

A

A

T

A

S

N

N

P

P

Y

Y

L

L

A

A

F

F

A

A

A

A

L

M

V

L

M

C

A

A

G

G

L

I

D

D

G

G

I

I

Q

K

N

N

K

Q

I

I

H

D

P

P

G

G

D

E

A

P

A

L

D

D

K

V

N

D

L

I

Y

Y

D

D

L

L

P

S

P

P

E

E

E

E

A

L

K

A

E

K

I

I

P

P

Q

S

V

T

C

P

G

G

S

S

L

L

K

E

E

A

A

A

L

L

E

E

E

A

L

L

D

E

K

K

G

D

R

H

A

E

F

F

L

L

T

T

K

G

G

T

G

G

V

V

F

F

S

S

D

P

D

D

F

F

I

V

D

E

A

S

Y

W

I

I

G

E

L

Y

K

K

S

L

E

D

E

N

E

E

I

V

K

N

-

P

V

M

R

R

T

L

F

R

V

P

H

H

P

P

L

Y

E

E

Y

F

E

S

L

L

Y

Y

Y

Y

S

D

C

C

E134 (= E131) binding Mg(2+)
E215 (= E212) binding Mg(2+)
E223 (= E220) binding Mg(2+)
E361 (= E357) binding Mn(2+)

3ng0A Crystal structure of glutamine synthetase from synechocystis sp. Pcc 6803
52% identity, 99% coverage: 5:468/468 of query aligns to 7:465/465 of 3ng0A

query
sites
3ng0A

V

L

Q

K

L

W

I

I

K

Q

D

D

H

E

D

N

V

I

K

K

W

I

I

I

D

D

L

L

R

K

F

F

T

I

D

D

T

T

K

P

G

G

T

I

Q

W

H

Q

H

H

V

C

T

S

M

F

P

-

A

Y

R

Y

D

D

A

Q

L

L

D

D

E

E

A

N

F

S

F

F

E

T

E

E

G

G

K

I

M

P

F

F

D
|
D

G

G

S

S

S

S

I

I

A

R

G

G

W

W

K

K

G

A

I

I

E

N

A

E

S

S

D

D

M

M

I

C

L

M

M

V

P

P

D

D

D

P

S

N

T

T

A

A

V

T

L

I

D

D

P

P

F

F

T

C

E

K

E

E

P

P

T

T

L

L

I

S

L

M

V

I

C

C

D

S

V

I

I

K

E

E

P

P

S

R

T

T

M

G

Q

E

G

W

Y

Y

D

N

R

R

D

D

P

P

R

R

A

T

I

I

A

A

K

A

R

K

A

A

E

A

E

E

Y

Y

L

L

K

R

S

G

T

T

G

G

I

I

G

A

D

D

T

T

V

V

F
x
Y

V

F

G

G

P

P

E
|
E

P

A

E
|
E

F

F

F

F

I

L

F

F

D

D

Q

D

V

I

K

R

F

F

K

G

S

Q

D

T

I

E

S

N

G

S

S

S

M

Y

F

Y

K

F

I

A

Y

D

S

S

E

V

Q

E

G

G

S

R

W

W

M

N

S

T

D

G

Q

R

D

E

V

E

E

E

G

G

G

G

N

N

H

L

G

G

H

Y

R

K

P

P

G

G

I

Y

K

K

G

Q

G

G

Y

Y

F

F

P

P

V

V

P

A

P

P

F

T

D

D

H

T

D

A

H

Q

E

D

I

I

R

R

T

T

S

E

M

M

C

L

N

L

A

T

M

M

E

A

D

A

M

F

G

G

L

V

T

P

I

I

E

E

V
x
K

H

H

H

H

H

H

E
|
E

V

V

A

A

T

S

A

G

G

G

Q

Q

N

N

E
|
E

I

L

G

G

V
x
I

K

K

F
|
F

N

D

T

K

L

L

V

V

A

N

K

S

A

A

D

D

E

N

V

L

Q

M

T

I

L

Y

K

K

Y

Y

C

V

V

I

H

K

N

N

T

V

A

A

V

K

A

K

Y

Y

G

G

R

K

T

T

A

V

T

T

F

F

M

M

P

P

K

K

P

P

L

I

Y

F

G

N

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

V

H
|
H

L

Q

S
|
S

I

L

A

W

K

K

E

D

G

G

K

Q

N

P

T

L

F

F

A

A

G

G

E

D

G

K

Y

Y

A

A

G

G

L

F

S

S

D

Q

T

M

A

G

L

L

Y

W

F

Y

I

I

G

G

G

G

I

I

I

L

K

K

H

H

G

A

K

P

A

A

L

L

N

L

G

A

F

F

T

T

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

F

E

E

A

A

P

P

V

V

M

N

L

L

A

A

Y

Y

S

S

A

Q

R

G

N

N

R
|
R

S

S

A

A

S

S

I

V

R
|
R

I

I

P

P

Y

L

V

S

-

G

S

G

S

N

P

P

R

K

A

A

R

K

R
|
R

I

L

E
|
E

A

F

R
|
R

F

C

P

P

D

D

P

A

A

T

A

S

N

N

P

P

Y

Y

L

L

A

A

F

F

A

A

A

A

L

M

V

L

M

C

A

A

G

G

L

I

D

D

G

G

I

I

Q

K

N

N

K

Q

I

I

H

D

P

P

G

G

D

E

A

P

A

L

D

D

K

V

N

D

L

I

Y

-

D

-

L

-

P

-

P

-

E

-

E

E

A

L

K

A

E

K

I

I

P

P

Q

S

V

T

C

P

G

G

S

S

L

L

K

E

E

A

A

A

L

L

E

E

E

A

L

L

D

E

K

K

G

D

R

H

A

E

F

F

L

L

T

T

K

G

G

T

G

G

V

V

F

F

S

S

D

P

D

D

F

F

I

V

D

E

A

S

Y

W

I

I

G

E

L

Y

K

K

S

L

E

D

E

N

E

E

I

V

K

N

-

P

V

M

R

R

T

L

F

R

V

P

H

H

P

P

L

Y

E

E

Y

F

E

S

L

L

Y

Y

Y

Y

S

D

C

C

active site: D51 (= D50), E130 (= E129), E132 (= E131), E213 (= E212), E221 (= E220), H270 (= H269), R340 (= R339), E359 (= E357), R361 (= R359)
binding phosphoaminophosphonic acid-adenylate ester: Y126 (≠ F125), E130 (= E129), K209 (≠ V208), I224 (≠ V223), F226 (= F225), H272 (= H271), S274 (= S273), R340 (= R339), R345 (= R344), R357 (= R355)
binding manganese (ii) ion: E130 (= E129), E132 (= E131), E213 (= E212), E221 (= E220), H270 (= H269), E359 (= E357), R361 (= R359)

A0R079 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
51% identity, 99% coverage: 6:466/468 of query aligns to 11:476/478 of A0R079

query
sites
A0R079

Q

K

L

L

I

I

K
|
K

D

D

H

E

D

N

V

V

K

E

W

Y

I

V

D

D

L

I

R

R

F

F

T

C

D

D

T

L

K

P

G

G

T

V

Q

V

H

Q

H

H

V

F

T

S

M

I

P

P

A

A

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-

D

S

A

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D

D

E

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L

Y

Y

Y

Y

K14 (= K9) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

5zlpL Crystal structure of glutamine synthetase from helicobacter pylori (see paper)
49% identity, 99% coverage: 6:468/468 of query aligns to 12:476/476 of 5zlpL

query
sites
5zlpL

Q

E

L

F

I

C

K

K

D

E

H

N

D

E

V

V

K

E

W

F

I

V

D

D

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R

R

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D

D

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G

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M

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F

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G

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G

G

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W

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G

G

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I

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D

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M

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L

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C

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Q

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I

I

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K

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K

A

A

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K

S

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T

S

G

G

I

L

G

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D

D

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V

V

A

F

Y

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G
|
G

P

A

E
|
E

P

N

E
|
E

F

F

F

F

I

I

F

F

D

D

Q

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I

K

K

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K

K

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D

A

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N

G

S

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M

Y

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G

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W

W

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N

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D

D

Q

R

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N

G

G

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V

N

N

H

F

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G

H

H

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P

G

G

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K

K

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G

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M

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C

V

N

K

A

V

M

L

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N

D

Q

M

V

G

G

L

L

T

E

I

T

E
x
F

V

V

H

V

H

H

H

H

E
|
E

V

V

A

A

T

Q

A

A

G

-

Q

Q

N

G

E
|
E

I

V

G

G

V

V

K

K

F
|
F

N

G

T

D

L

L

V

V

A

E

K

A

A

A

D

D

E

N

V

V

Q

Q

T

K

L

L

K

K

Y

Y

C

V

V

V

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K

N

M

T

V

A

A

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A

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Y

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G

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K

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T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

Y

Y

G

G

D

D

N

N

G
|
G

S

S

G

G

M

M

H
|
H

V

T

H
|
H

L

V

S
|
S

I

V

A

W

K

K

E

N

G

N

K

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N

N

T

L

F

F

A

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G

G

E

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G

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Y

Y

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G

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G

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L

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A

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F

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N

N

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A

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S

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

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P

P

G

G

F

Y

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|
E

A

A

P

P

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S

M

I

L

L

A

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Y

Y

S

S

A

A

R

N

N

N

R
|
R

S

S

A

A

S

S

I

V

R
|
R

I

I

P

P

Y

Y

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G

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I

S

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K

R

N

A

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A

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|
R

I

F

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A

F

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|
R

F

F

P

P

D

D

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A

S

A

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N

N

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P

Y

Y

L

L

A

A

F

F

A

A

A

A

L

I

V

L

M

M

A

A

G

G

L

M

D

D

G

G

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N

N

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K

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D