SitesBLAST
Comparing GFF3754 FitnessBrowser__Marino:GFF3754 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A3SI50 3-methylmercaptopropionyl-CoA dehydrogenase; MMPA-CoA dehydrogenase; EC 1.3.99.41 from Roseovarius nubinhibens (strain ATCC BAA-591 / DSM 15170 / ISM) (see paper)
44% identity, 87% coverage: 84:683/689 of query aligns to 1:589/591 of A3SI50
- M161 (= M246) mutation to A: Retains 37% of wild-type activity.
- T170 (= T255) mutation to A: Retains 8.8% of wild-type activity.
- F195 (= F280) mutation to A: Almost completely abolishes the activity.
- S197 (= S282) mutation to A: Retains 3.6% of wild-type activity.
- K223 (≠ R308) mutation to A: Retains 9.4% of wild-type activity.
- H280 (≠ N365) mutation to A: Retains 18% of wild-type activity.
- K281 (≠ D366) mutation to A: Retains 54% of wild-type activity.
- R284 (≠ N369) mutation to A: Retains 97% of wild-type activity.
- F287 (= F372) mutation to A: Retains 76% of wild-type activity.
- Y434 (= Y526) mutation to A: Retains 51% of wild-type activity.
- E435 (= E527) mutation to A: Loss of activity.
- R448 (= R540) mutation to A: Retains 44% of wild-type activity.
Q3L887 Broad-specificity linear acyl-CoA dehydrogenase FadE5; Long-chain-acyl-CoA dehydrogenase; Medium-chain-acyl-CoA dehydrogenase; Short-chain-acyl-CoA dehydrogenase; EC 1.3.8.8; EC 1.3.8.7; EC 1.3.8.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
34% identity, 87% coverage: 84:681/689 of query aligns to 1:607/611 of Q3L887
6ksaB Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c18coa (see paper)
34% identity, 87% coverage: 83:681/689 of query aligns to 3:607/611 of 6ksaB
- binding flavin-adenine dinucleotide: M165 (= M246), L167 (= L248), T168 (= T249), G173 (= G254), S174 (≠ T255), F199 (= F280), I200 (= I281), T201 (≠ S282), R329 (= R404), I348 (≠ L423), H351 (= H426), Q423 (= Q500), T424 (≠ V501), G426 (= G503), G427 (= G504), I445 (= I522), Y449 (= Y526), T452 (= T529)
- binding magnesium ion: H388 (≠ E463), Y475 (≠ E553), E479 (≠ N557)
- binding stearoyl-coenzyme a: W115 (≠ L194), M133 (= M209), M137 (= M218), A163 (≠ G244), M165 (= M246), L167 (= L248), S174 (≠ T255), V176 (≠ L257), T227 (= T307), K228 (≠ R308), I293 (≠ L368), F297 (= F372), Q302 (≠ T377), R304 (= R379), Y449 (= Y526), A450 (≠ E527), I455 (= I532), D459 (= D536), R463 (= R540), K464 (= K541)
6lq8A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c22coa (see paper)
34% identity, 87% coverage: 84:681/689 of query aligns to 1:603/607 of 6lq8A
- binding coenzyme a: M162 (= M246), L164 (= L248), S171 (≠ T255), V173 (≠ L257), T224 (= T307), K225 (≠ R308), I290 (≠ L368), F294 (= F372), R301 (= R379), A447 (≠ E527), G448 (= G528), I452 (= I532), D456 (= D536), R460 (= R540), K461 (= K541)
- binding docosanoic acid: G96 (≠ S178), L97 (≠ A179), Q111 (≠ G193), M130 (= M209), G133 (= G212), M134 (= M218), E136 (≠ T220), I137 (= I221), M162 (= M246), T198 (≠ S282), Q299 (≠ T377), A300 (= A378), Y446 (= Y526), A447 (≠ E527)
- binding flavin-adenine dinucleotide: M162 (= M246), L164 (= L248), T165 (= T249), G170 (= G254), S171 (≠ T255), F196 (= F280), I197 (= I281), T198 (≠ S282), R326 (= R404), I345 (≠ L423), H348 (= H426), Q420 (= Q500), T421 (≠ V501), G423 (= G503), G424 (= G504), I442 (= I522), Y446 (= Y526), T449 (= T529)
6lq7A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c17coa (see paper)
34% identity, 87% coverage: 84:681/689 of query aligns to 1:603/607 of 6lq7A
- binding coenzyme a: M162 (= M246), L164 (= L248), S171 (≠ T255), V173 (≠ L257), T224 (= T307), K225 (≠ R308), I290 (≠ L368), F294 (= F372), R301 (= R379), A447 (≠ E527), G448 (= G528), I452 (= I532), D456 (= D536), R460 (= R540), K461 (= K541)
- binding flavin-adenine dinucleotide: M162 (= M246), L164 (= L248), T165 (= T249), G170 (= G254), S171 (≠ T255), F196 (= F280), I197 (= I281), T198 (≠ S282), R326 (= R404), I345 (≠ L423), H348 (= H426), Q420 (= Q500), T421 (≠ V501), G423 (= G503), G424 (= G504), I442 (= I522), Y446 (= Y526), T449 (= T529)
- binding heptadecanoic acid: M130 (= M209), A160 (≠ G244), Q299 (≠ T377), Y446 (= Y526), A447 (≠ E527)
6lq6A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c20coa (see paper)
34% identity, 87% coverage: 84:681/689 of query aligns to 1:603/607 of 6lq6A
- binding coenzyme a: M162 (= M246), L164 (= L248), S171 (≠ T255), V173 (≠ L257), T224 (= T307), K225 (≠ R308), I290 (≠ L368), F294 (= F372), R301 (= R379), A447 (≠ E527), G448 (= G528), I452 (= I532), D456 (= D536), R460 (= R540), K461 (= K541)
- binding icosanoic acid: G96 (≠ S178), M130 (= M209), G133 (= G212), M134 (= M218), E136 (≠ T220), A160 (≠ G244), Q299 (≠ T377), M303 (≠ G381), A447 (≠ E527)
- binding flavin-adenine dinucleotide: M162 (= M246), L164 (= L248), T165 (= T249), G170 (= G254), S171 (≠ T255), F196 (= F280), I197 (= I281), T198 (≠ S282), T266 (= T348), R326 (= R404), H348 (= H426), Q420 (= Q500), T421 (≠ V501), G423 (= G503), G424 (= G504), I442 (= I522), Y446 (= Y526), T449 (= T529)
6lq1A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c8coa (see paper)
34% identity, 87% coverage: 84:681/689 of query aligns to 1:603/607 of 6lq1A
- binding coenzyme a: M162 (= M246), S171 (≠ T255), V173 (≠ L257), T224 (= T307), I290 (≠ L368), F294 (= F372), R301 (= R379), A447 (≠ E527), I452 (= I532), D456 (= D536), R460 (= R540), K461 (= K541)
- binding flavin-adenine dinucleotide: L164 (= L248), T165 (= T249), G170 (= G254), S171 (≠ T255), F196 (= F280), I197 (= I281), T198 (≠ S282), T266 (= T348), R326 (= R404), H348 (= H426), Q420 (= Q500), T421 (≠ V501), G423 (= G503), G424 (= G504), I442 (= I522), Y446 (= Y526), T449 (= T529)
- binding octanoic acid (caprylic acid): M130 (= M209), M162 (= M246), Y446 (= Y526), A447 (≠ E527)
6lq0A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c6coa (see paper)
34% identity, 87% coverage: 84:681/689 of query aligns to 1:603/607 of 6lq0A
- binding hexanoic acid: M303 (≠ G381), Y446 (= Y526), A447 (≠ E527)
- binding coenzyme a: S171 (≠ T255), V173 (≠ L257), T224 (= T307), K225 (≠ R308), I290 (≠ L368), F294 (= F372), R301 (= R379), A447 (≠ E527), G448 (= G528), I452 (= I532), D456 (= D536), R460 (= R540), K461 (= K541)
- binding flavin-adenine dinucleotide: M162 (= M246), L164 (= L248), T165 (= T249), G170 (= G254), S171 (≠ T255), F196 (= F280), I197 (= I281), T198 (≠ S282), R326 (= R404), I345 (≠ L423), H348 (= H426), Q420 (= Q500), T421 (≠ V501), G423 (= G503), G424 (= G504), I442 (= I522), Y446 (= Y526), T449 (= T529), Q455 (≠ L535)
6lpyA Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c4coa (see paper)
34% identity, 87% coverage: 84:681/689 of query aligns to 1:603/607 of 6lpyA
- binding butanoic acid: Y446 (= Y526), A447 (≠ E527)
- binding coenzyme a: M162 (= M246), L164 (= L248), S171 (≠ T255), V173 (≠ L257), T224 (= T307), K225 (≠ R308), I290 (≠ L368), F294 (= F372), R301 (= R379), A447 (≠ E527), I452 (= I532), D456 (= D536), R460 (= R540), K461 (= K541), R464 (≠ M545)
- binding flavin-adenine dinucleotide: M162 (= M246), L164 (= L248), T165 (= T249), G170 (= G254), S171 (≠ T255), F196 (= F280), I197 (= I281), T198 (≠ S282), R326 (= R404), I345 (≠ L423), H348 (= H426), Q420 (= Q500), T421 (≠ V501), G423 (= G503), G424 (= G504), I442 (= I522), Y446 (= Y526), T449 (= T529)
6ksbA Crystal structure of e447a m130g acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c16coa (see paper)
34% identity, 87% coverage: 84:681/689 of query aligns to 1:604/608 of 6ksbA
- binding coenzyme a: M162 (= M246), S171 (≠ T255), V173 (≠ L257), T224 (= T307), K225 (≠ R308), I290 (≠ L368), F294 (= F372), E298 (≠ N376), R301 (= R379), A447 (≠ E527), G448 (= G528), I452 (= I532), D456 (= D536), R460 (= R540), K461 (= K541)
- binding flavin-adenine dinucleotide: L164 (= L248), T165 (= T249), G170 (= G254), S171 (≠ T255), F196 (= F280), I197 (= I281), T198 (≠ S282), T266 (= T348), R326 (= R404), I345 (≠ L423), H348 (= H426), Q420 (= Q500), T421 (≠ V501), G423 (= G503), G424 (= G504), I442 (= I522), Y446 (= Y526), T449 (= T529), Q455 (≠ L535)
O53666 Broad-specificity linear acyl-CoA dehydrogenase FadE5; Long-chain-acyl-CoA dehydrogenase; Medium-chain-acyl-CoA dehydrogenase; Short-chain-acyl-CoA dehydrogenase; EC 1.3.8.8; EC 1.3.8.7; EC 1.3.8.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 87% coverage: 84:681/689 of query aligns to 1:607/611 of O53666
- MVLT 162:165 (≠ MCLT 246:249) binding
- S171 (≠ T255) binding ; binding ; mutation to A: Decreases affinity for eicosanoyl-CoA; when associated with A-447.
- T198 (≠ S282) binding
- TK 224:225 (≠ TR 307:308) binding
- K225 (≠ R308) mutation to A: Decreases affinity for eicosanoyl-CoA; when associated with A-447.
- F294 (= F372) mutation to A: Increases affinity for eicosanoyl-CoA; when associated with A-447.
- R301 (= R379) binding ; mutation to A: Increases affinity for eicosanoyl-CoA; when associated with A-447.
- R326 (= R404) binding
- K338 (≠ P416) binding
- QTLGG 420:424 (≠ QVFGG 500:504) binding
- E447 (= E527) binding ; mutation to A: Loss of activity.
- T449 (= T529) binding
- D456 (= D536) binding
- R460 (= R540) mutation to A: Decreases affinity for eicosanoyl-CoA; when associated with A-447.
- RK 460:461 (= RK 540:541) binding
6kseA Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria tuberculosisin complex with c18coa (see paper)
34% identity, 87% coverage: 84:681/689 of query aligns to 1:603/607 of 6kseA
- active site: L164 (= L248), T165 (= T249), A300 (= A378), R460 (= R540)
- binding flavin-adenine dinucleotide: M162 (= M246), L164 (= L248), T165 (= T249), G170 (= G254), S171 (≠ T255), F196 (= F280), T198 (≠ S282), R326 (= R404), Q328 (≠ S406), I345 (≠ L423), H348 (= H426), Q420 (= Q500), T421 (≠ V501), G423 (= G503), G424 (= G504), I442 (= I522), Y446 (= Y526), T449 (= T529)
- binding stearoyl-coenzyme a: D93 (≠ Q175), H115 (≠ Q197), W126 (≠ S208), G130 (= G212), G133 (= G216), F134 (≠ A217), M162 (= M246), S171 (≠ T255), T224 (= T307), K225 (≠ R308), I290 (≠ L368), F294 (= F372), A300 (= A378), R301 (= R379), Y446 (= Y526), A447 (≠ E527), I452 (= I532), D456 (= D536), R460 (= R540), K461 (= K541)
4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
31% identity, 54% coverage: 173:544/689 of query aligns to 49:378/380 of 4l1fA
- active site: L125 (= L248), T126 (= T249), G242 (≠ A378), E363 (= E527), R375 (≠ K541)
- binding coenzyme a persulfide: T132 (= T255), H179 (≠ R308), F232 (≠ L368), M236 (≠ F372), E237 (≠ T373), L239 (≠ M375), D240 (≠ N376), R243 (= R379), Y362 (= Y526), E363 (= E527), G364 (= G528), R375 (≠ K541)
- binding flavin-adenine dinucleotide: F123 (≠ M246), L125 (= L248), T126 (= T249), G131 (= G254), T132 (= T255), F156 (= F280), I157 (= I281), T158 (≠ S282), R268 (= R404), Q270 (= Q418), F271 (≠ V419), I275 (≠ L423), F278 (≠ H426), L281 (≠ V429), Q336 (= Q500), I337 (≠ V501), G340 (= G504), I358 (= I522), Y362 (= Y526), T365 (= T529), Q367 (= Q533)
Sites not aligning to the query:
5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
30% identity, 53% coverage: 173:535/689 of query aligns to 46:366/374 of 5lnxD
- active site: L122 (= L248), T123 (= T249), G239 (≠ A378), E358 (= E527)
- binding flavin-adenine dinucleotide: L122 (= L248), T123 (= T249), G128 (= G254), S129 (≠ T255), F153 (= F280), T155 (≠ S282), R265 (= R404), Q267 (= Q418), F268 (≠ V419), I272 (≠ L423), N275 (≠ H426), I278 (≠ V429), Q331 (= Q500), I332 (≠ V501), G335 (= G504), Y357 (= Y526), T360 (= T529), E362 (≠ G531)
Sites not aligning to the query:
5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
29% identity, 59% coverage: 135:540/689 of query aligns to 9:375/378 of 5ol2F
- active site: L124 (= L248), T125 (= T249), G241 (≠ A378), G374 (= G539)
- binding calcium ion: E29 (= E155), E33 (≠ P159), R35 (≠ G161)
- binding coenzyme a persulfide: L238 (≠ M375), R242 (= R379), E362 (= E527), G363 (= G528)
- binding flavin-adenine dinucleotide: F122 (≠ M246), L124 (= L248), T125 (= T249), P127 (= P251), T131 (= T255), F155 (= F280), I156 (= I281), T157 (≠ S282), E198 (= E338), R267 (= R404), F270 (≠ V419), L274 (= L423), F277 (≠ H426), Q335 (= Q500), L336 (≠ V501), G338 (= G503), G339 (= G504), Y361 (= Y526), T364 (= T529), E366 (≠ G531)
4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
29% identity, 53% coverage: 173:535/689 of query aligns to 50:372/378 of 4n5fA
- active site: L126 (= L248), T127 (= T249), G243 (≠ A378), E364 (= E527)
- binding dihydroflavine-adenine dinucleotide: L126 (= L248), T127 (= T249), G132 (= G254), S133 (≠ T255), F157 (= F280), T159 (≠ S282), T210 (= T348), Y363 (= Y526), T366 (= T529), E368 (≠ G531), M372 (≠ L535)
Sites not aligning to the query:
2dvlA Crystal structure of project tt0160 from thermus thermophilus hb8
29% identity, 53% coverage: 173:535/689 of query aligns to 45:362/370 of 2dvlA
- active site: L121 (= L248), T122 (= T249), G233 (≠ A378), E354 (= E527)
- binding flavin-adenine dinucleotide: L121 (= L248), T122 (= T249), G127 (= G254), S128 (≠ T255), W152 (≠ F280), I153 (= I281), T154 (≠ S282), T356 (= T529), E358 (≠ G531)
Sites not aligning to the query:
3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
29% identity, 54% coverage: 173:543/689 of query aligns to 41:368/369 of 3pfdC
- active site: L116 (= L248), S117 (≠ T249), T233 (≠ A378), E353 (= E527), R365 (= R540)
- binding dihydroflavine-adenine dinucleotide: Y114 (≠ M246), L116 (= L248), S117 (≠ T249), G122 (= G254), S123 (≠ T255), W147 (≠ F280), I148 (= I281), T149 (≠ S282), R259 (= R404), F262 (≠ V419), V266 (≠ L423), N269 (≠ H426), Q326 (= Q500), L327 (≠ V501), G330 (= G504), I348 (= I522), Y352 (= Y526), T355 (= T529), Q357 (≠ G531)
P50544 Very long-chain specific acyl-CoA dehydrogenase, mitochondrial; MVLCAD; VLCAD; EC 1.3.8.9 from Mus musculus (Mouse) (see paper)
29% identity, 64% coverage: 95:535/689 of query aligns to 82:471/656 of P50544
- C238 (≠ D269) modified: S-nitrosocysteine
8ca1B Cryo-em structure of the acadvl dimer from mus musculus. (see paper)
29% identity, 64% coverage: 95:535/689 of query aligns to 15:404/589 of 8ca1B
- binding flavin-adenine dinucleotide: F148 (≠ M246), T151 (= T249), G156 (= G254), S157 (≠ T255), W183 (≠ F280), S185 (= S282), R300 (= R408), Q302 (≠ L410), F303 (≠ S411), I307 (= I424), V312 (= V429), I313 (≠ R430), Q369 (= Q500), I370 (≠ V501), F395 (≠ Y526), D400 (≠ G531), I401 (= I532)
Query Sequence
>GFF3754 FitnessBrowser__Marino:GFF3754
MTFTLSEQENVGVLGARKYAETLFSDHYPESTISATSSRPIVHSDSLPGAFRQWQCPGIG
VKHKATNGGCPPKIVEKDTEDSAMPVYKAPLRDMKFLLNEVFDYPGHYATLTSGENATPD
IVDAILGECGKFCEEVLSPLYLSGDEEGCKLDNGEVTTPAGYRDAYQQYAMGGWQGLSAP
EEYGGQGLPASMGLLKQEMMGTANWPFSMYPGLSLGAMNTIFLHGTEDQKQTYLAPLTEG
RWAGTMCLTEPQCGTDLGQVKTKAEPQADGSYRLTGTKIFISSGDHDLTENIVHIVLARL
PDAPKGTRGISLFIVPKFLPDSNGDVGERNGVVCGSLEKKMGIKASATCVLNFDGATGFL
IGPENDGLNCMFTFMNTARIGTAIQGVGPAELSYQWALAYAKDRRSMRALSGKKEPDQVA
DSLIHHADVRRMLLTQKAIAEGGRAMLYYAARLADHMVEGFTEGDEKKADKYDDKLGFLT
PILKGFLTELGCEAANLGVQVFGGHGYIREHGMEQIVRDTRIATLYEGTTGIQALDLLGR
KVLLMTQGGAVREFTLNIANFARKHLTDKRLRPWALELLKLTGQWNLLTVRIMLAARKDR
DVVSSASYDFLMYSGYVTMAYIWLRQAAVAVDKLDNGGEESTGFYQAKLATTEFYFERLL
PRAQSHATSMLSPSKNLMQLDAEDLAFTG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory