SitesBLAST

CV 59:60 (≠ YT 38:39) binding
EK 80:81 (≠ EA 58:59) binding
87:95 (vs. 65:73, 33% identical) binding
H91 (≠ R69) modified: Tele-8alpha-FAD histidine
H109 (≠ E91) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
V219 (= V208) binding

Sites not aligning to the query:

279 S → P: in dbSNP:rs532964
397:402 binding
530 A → G: in dbSNP:rs1805073
646 S → P: in dbSNP:rs1805074

Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 55% coverage: 29:265/433 of query aligns to 43:272/857 of Q63342

query
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Q63342

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CV 52:53 (≠ YT 38:39) binding
EK 73:74 (≠ EA 58:59) binding
80:88 (vs. 65:73, 33% identical) binding
H84 (≠ R69) modified: Tele-8alpha-FAD histidine
V212 (= V208) binding
W244 (≠ Y239) binding

Sites not aligning to the query:

390:395 binding
573:575 binding
669 binding
676:678 binding
737 binding

4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
26% identity, 55% coverage: 29:265/433 of query aligns to 6:235/824 of 4pabB

query
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4pabB

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active site: T53 (≠ N75), E102 (≠ T126), H226 (≠ Y256)
binding flavin-adenine dinucleotide: I11 (≠ V34), G12 (= G35), G14 (= G37), C15 (≠ Y38), V16 (≠ T39), L35 (= L57), E36 (= E58), K37 (≠ A59), G43 (= G65), S44 (≠ G66), T45 (≠ S67), H47 (≠ R69), A48 (≠ N70), A49 (≠ V71), G50 (= G72), L51 (= L73), V175 (= V208), A204 (≠ T236), G205 (≠ N237), W207 (≠ Y239), H226 (≠ Y256), Y228 (≠ Q258)

Sites not aligning to the query:

active site: 255, 536
binding flavin-adenine dinucleotide: 326, 328, 353, 355, 356, 357, 358
binding (6s)-5,6,7,8-tetrahydrofolate: 523, 536, 538, 550, 612, 613, 632, 639, 680, 700

1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
21% identity, 64% coverage: 123:400/433 of query aligns to 83:368/374 of 1y56B

query
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1y56B

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active site: H224 (vs. gap), P239 (≠ R271), G305 (= G339), M338 (≠ G369)
binding flavin-adenine dinucleotide: E170 (≠ P207), V171 (= V208), T200 (= T236), N201 (= N237), W203 (≠ Y239), G305 (= G339), Y306 (≠ K340), Y307 (≠ I341), G334 (= G365), H335 (≠ R366), G336 (= G367), F337 (≠ I368), M338 (≠ G369)
binding flavin mononucleotide: I260 (≠ A294), R301 (≠ A335), W303 (= W337)

Sites not aligning to the query:

active site: 44, 47, 48
binding flavin-adenine dinucleotide: 11, 13, 14, 15, 33, 34, 35, 42, 43, 45, 46, 47, 49
binding flavin mononucleotide: 44, 45

Query Sequence

>GFF3817 FitnessBrowser__Phaeo:GFF3817
MTPDRNRSLWQATCREDLDAAALHDDVSADLVVVGGGYTGCSAALRAAELGADVRLLEAE
TFGAGGSGRNVGLVNAGLWLPPKKINSVLGEVNGTRLSTLLAAAPAAVFELIKTHQIDCE
PIQTGTLHCAHARSGLKDLRKRHQQLTAIGAPVTLLSRGDAIARVGSDAVYGALFDPRAG
TIQPLAYAIGLARAAASQGARLHASSPVVGLTRETDGWRVSTPGGTVRARHLIMATNAYP
LPITGYTAPATIPVSYFQAATAPLPAGLAGRILPQAEGCWDTGLIMSSWRRDRAGRLIIG
GMGDLSHPARSVHTNWLRRKLAVMFPALADHPFEAEWAGKIAMTTEHIPKIIDLGQGFAC
FGYSGRGIGPGTVFGRRMAEALMHGDRSLLPIPVSDDHRLPFAGVRGTYYETGATLVHLL
AGRVGQTPTPTDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory