SitesBLAST
Comparing GFF4170 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6ztlAP coli 70S-RNAP expressome complex in collided state bound to NusG (see paper)
98% identity, 100% coverage: 1:82/82 of query aligns to 1:82/82 of 6ztlAP
- binding magnesium ion: E34 (= E34), R35 (= R35), V36 (= V36)
- binding : M1 (= M1), R5 (= R5), L6 (= L6), R8 (= R8), G10 (= G10), K12 (= K12), K13 (= K13), R14 (= R14), F16 (= F16), S24 (= S24), R25 (= R25), R28 (= R28), G30 (= G30), R31 (= R31), R35 (= R35), F38 (= F38), I42 (= I42), R51 (= R51), Q63 (= Q63), G64 (= G64), S68 (= S68), R70 (= R70)
4a2iP Cryo-electron microscopy structure of the 30s subunit in complex with the yjeq biogenesis factor (see paper)
98% identity, 100% coverage: 1:82/82 of query aligns to 1:82/82 of 4a2iP
- binding : M1 (= M1), R5 (= R5), L6 (= L6), R8 (= R8), H9 (= H9), K12 (= K12), K13 (= K13), R14 (= R14), F16 (= F16), Y17 (= Y17), D23 (= D23), S24 (= S24), R25 (= R25), R28 (= R28), G30 (= G30), R31 (= R31), R35 (= R35), P41 (= P41), W60 (= W60), G64 (= G64), S68 (= S68), R70 (= R70), E77 (= E77)
7m4up A. Baumannii ribosome-eravacycline complex: 30s (see paper)
72% identity, 99% coverage: 1:81/82 of query aligns to 1:81/83 of 7m4up
- binding : M1 (= M1), V2 (= V2), R5 (= R5), L6 (= L6), G10 (= G10), K12 (= K12), K13 (= K13), R14 (= R14), F16 (= F16), Y17 (= Y17), R25 (= R25), R28 (= R28), G30 (= G30), R31 (= R31), R35 (= R35), Q44 (≠ S44), Q46 (≠ K46), Q63 (= Q63), G64 (= G64), Q66 (≠ T66), S68 (= S68), R70 (= R70)
7unrp 30S ribosomal protein S6
60% identity, 99% coverage: 1:81/82 of query aligns to 1:81/81 of 7unrp
- binding : M1 (= M1), R5 (= R5), L6 (= L6), R8 (= R8), G10 (= G10), K12 (= K12), K13 (= K13), R14 (= R14), Y17 (= Y17), N23 (≠ D23), R25 (= R25), R28 (= R28), G30 (= G30), R31 (= R31), E47 (= E47), Q63 (= Q63), G64 (= G64), R70 (= R70), Q73 (≠ A73)
8rd8Pj Small ribosomal subunit protein uS5 (see paper)
60% identity, 99% coverage: 1:81/82 of query aligns to 1:81/81 of 8rd8Pj
- binding : M1 (= M1), V2 (= V2), R5 (= R5), L6 (= L6), R8 (= R8), G10 (= G10), K12 (= K12), K13 (= K13), R14 (= R14), F16 (= F16), Y17 (= Y17), Q18 (= Q18), D23 (= D23), Q24 (≠ S24), R25 (= R25), R26 (≠ N26), R28 (= R28), G30 (= G30), R31 (= R31), F38 (= F38), K44 (≠ S44), E47 (= E47), R51 (= R51), K63 (≠ Q63), G64 (= G64), Q66 (≠ T66), S68 (= S68), R70 (= R70), S73 (≠ A73), R76 (≠ K76)
8fmwP 30S ribosomal protein S10 (see paper)
46% identity, 90% coverage: 2:75/82 of query aligns to 3:74/83 of 8fmwP
- binding : V3 (= V2), K4 (≠ T3), R6 (= R5), M10 (≠ H9), G11 (= G10), K13 (= K12), K14 (= K13), R15 (= R14), Y17 (≠ F16), Y18 (= Y17), R19 (≠ Q18), N24 (≠ D23), T26 (≠ R25), P28 (≠ A27), R29 (= R28), G31 (= G30), R32 (= R31), E44 (= E45), K62 (≠ Q63), G63 (= G64), I65 (≠ T66), M72 (≠ A73)
Sites not aligning to the query:
3j9wAP 30S ribosomal protein uS9 (see paper)
50% identity, 90% coverage: 2:75/82 of query aligns to 2:73/89 of 3j9wAP
Sites not aligning to the query:
5myjAP of 70S ribosome from Lactococcus lactis (see paper)
46% identity, 90% coverage: 2:75/82 of query aligns to 2:73/86 of 5myjAP
- binding : K3 (≠ T3), R5 (= R5), L6 (= L6), T7 (≠ A7), R8 (= R8), M9 (≠ H9), S11 (≠ A11), K12 (= K12), K13 (= K13), K14 (≠ R14), F16 (= F16), Y17 (= Y17), R18 (≠ Q18), D23 (= D23), R25 (= R25), R28 (= R28), G30 (= G30), K31 (≠ R31), F32 (= F32), I33 (= I33), T38 (≠ F38), L42 (≠ I42), W58 (= W60), S60 (≠ G62), N61 (≠ Q63), G62 (= G64), T68 (≠ R70), R70 (≠ A72), N71 (≠ A73)
Sites not aligning to the query:
7pntM 28S ribosomal protein S11, mitochondrial (see paper)
43% identity, 90% coverage: 2:75/82 of query aligns to 8:82/126 of 7pntM
- binding : R11 (= R5), L12 (= L6), T18 (≠ K12), N19 (≠ K13), Y23 (= Y17), H29 (≠ D23), N30 (≠ S24), K31 (≠ R25), R34 (= R28), G36 (= G30), R37 (= R31), F38 (= F32), W67 (= W60), C70 (≠ Q63), G71 (= G64), H73 (≠ T66), S75 (= S68), P77 (≠ R70)
Sites not aligning to the query:
6o8wp 30S ribosomal protein S10 (see paper)
47% identity, 90% coverage: 2:75/82 of query aligns to 2:73/89 of 6o8wp
- binding : K3 (≠ T3), R5 (= R5), L6 (= L6), R8 (= R8), M9 (≠ H9), S11 (≠ A11), K12 (= K12), R18 (≠ Q18), D23 (= D23), R25 (= R25), P27 (≠ A27), R28 (= R28), R31 (= R31), P41 (= P41), L42 (≠ I42), K43 (≠ A43), D44 (≠ S44), S66 (= S68), T68 (≠ R70), R70 (≠ A72), N71 (≠ A73)
Sites not aligning to the query:
8oisAM 28S ribosomal protein S35, mitochondrial (see paper)
43% identity, 90% coverage: 2:75/82 of query aligns to 8:82/119 of 8oisAM
- binding fe-s-o hybrid cluster: G16 (= G10), C17 (≠ A11)
- binding : R11 (= R5), L12 (= L6), T18 (≠ K12), N19 (≠ K13), Y23 (= Y17), H29 (≠ D23), N30 (≠ S24), K31 (≠ R25), P33 (≠ A27), R34 (= R28), R37 (= R31), F38 (= F32), W67 (= W60), C70 (≠ Q63), G71 (= G64), H73 (≠ T66), S75 (= S68), K76 (≠ D69), P77 (≠ R70)
Sites not aligning to the query:
7p2eM 28S ribosomal protein S9, mitochondrial (see paper)
43% identity, 90% coverage: 2:75/82 of query aligns to 8:82/119 of 7p2eM
- binding fe2/s2 (inorganic) cluster: C17 (≠ A11), R20 (= R14)
- binding : R11 (= R5), L12 (= L6), T18 (≠ K12), N19 (≠ K13), Y23 (= Y17), H29 (≠ D23), N30 (≠ S24), K31 (≠ R25), P33 (≠ A27), R34 (= R28), G36 (= G30), R37 (= R31), F38 (= F32), W67 (= W60), C70 (≠ Q63), G71 (= G64), H73 (≠ T66), S75 (= S68), P77 (≠ R70)
Sites not aligning to the query:
6xywBo At4g35490 (see paper)
44% identity, 91% coverage: 1:75/82 of query aligns to 1:76/99 of 6xywBo
- binding : R3 (≠ T3), R5 (= R5), L6 (= L6), R8 (= R8), F9 (≠ H9), C11 (≠ A11), K12 (= K12), N13 (≠ K13), R14 (= R14), F16 (= F16), F17 (≠ Y17), R18 (≠ Q18), D23 (= D23), S24 (= S24), R25 (= R25), R28 (= R28), H32 (≠ F32), Y38 (≠ F38), P43 (≠ A43), R50 (= R51), V64 (≠ Q63), G65 (= G64), Q67 (≠ T66), P71 (≠ R70), R74 (≠ A73)
Sites not aligning to the query:
1i94P Crystal structures of the small ribosomal subunit with tetracycline, edeine and if3 (see paper)
38% identity, 94% coverage: 1:77/82 of query aligns to 1:76/88 of 1i94P
- binding magnesium ion: K27 (≠ A27), R28 (= R28), D29 (≠ N29)
- binding : L6 (= L6), F9 (≠ H9), G10 (= G10), S11 (≠ A11), K12 (= K12), H16 (≠ F16), A24 (≠ S24), R25 (= R25), G30 (= G30), K31 (≠ R31), P41 (= P41), R42 (≠ I42), V62 (≠ Q63), G63 (= G64)
Sites not aligning to the query:
1hnwP Structure of the thermus thermophilus 30s ribosomal subunit in complex with tetracycline (see paper)
38% identity, 94% coverage: 1:77/82 of query aligns to 1:76/88 of 1hnwP
- binding : M1 (= M1), K3 (≠ T3), R5 (= R5), L6 (= L6), R8 (= R8), F9 (≠ H9), G10 (= G10), K12 (= K12), H13 (≠ K13), N14 (≠ R14), H16 (≠ F16), Y17 (= Y17), R18 (≠ Q18), D23 (= D23), R25 (= R25), K27 (≠ A27), R28 (= R28), K31 (≠ R31), K35 (≠ R35), P41 (= P41), R42 (≠ I42), K43 (≠ A43), T44 (≠ S44), T45 (≠ E45), W59 (= W60), V62 (≠ Q63), G63 (= G64), Q65 (≠ T66), T67 (≠ S68), T69 (≠ R70), R72 (≠ A73), R75 (≠ K76)
Sites not aligning to the query:
7nhnq 50S ribosomal protein L31 type B (see paper)
45% identity, 90% coverage: 2:75/82 of query aligns to 2:73/88 of 7nhnq
- binding : V2 (= V2), K3 (≠ T3), R5 (= R5), L6 (= L6), R8 (= R8), I9 (≠ H9), K12 (= K12), K13 (= K13), F16 (= F16), Y17 (= Y17), R18 (≠ Q18), D23 (= D23), S24 (= S24), R25 (= R25), R28 (= R28), G30 (= G30), R31 (= R31), L42 (≠ I42), N61 (≠ Q63), G62 (= G64), K64 (≠ T66), S66 (= S68), T68 (≠ R70), R70 (≠ A72), N71 (≠ A73)
Sites not aligning to the query:
7bgdp Staphylococcus aureus 30s ribosomal subunit in presence of spermidine (body only)
39% identity, 90% coverage: 2:75/82 of query aligns to 2:74/90 of 7bgdp
- binding : K3 (≠ T3), R5 (= R5), L6 (= L6), R8 (= R8), G10 (= G10), K12 (= K12), R13 (≠ K13), F16 (= F16), Y17 (= Y17), R18 (≠ Q18), D23 (= D23), R25 (= R25), P27 (≠ A27), R28 (= R28), G30 (= G30), R31 (= R31), P41 (= P41), W59 (= W60), D62 (≠ Q63), G63 (= G64), K65 (≠ T66), T67 (≠ S68), T69 (≠ R70), H71 (≠ A72), N72 (≠ A73)
Sites not aligning to the query:
8om2P 37S ribosomal protein S9, mitochondrial (see paper)
39% identity, 94% coverage: 1:77/82 of query aligns to 4:91/120 of 8om2P
- binding : L4 (≠ M1), V5 (= V2), R6 (≠ T3), R8 (= R5), L9 (= L6), R11 (= R8), R14 (≠ A11), K15 (= K12), N16 (≠ K13), Y20 (= Y17), N26 (≠ D23), R28 (= R25), R31 (= R28), A33 (≠ G30), K34 (≠ R31), P46 (≠ A43), K51 (vs. gap), R52 (vs. gap), K62 (vs. gap), G78 (= G64), Q80 (≠ T66), S82 (= S68), T84 (≠ R70), K87 (≠ A73)
Sites not aligning to the query:
8crxP 30S ribosomal protein S17 (see paper)
42% identity, 89% coverage: 4:76/82 of query aligns to 4:74/128 of 8crxP
- binding : R5 (= R5), L6 (= L6), G10 (= G10), K11 (≠ A11), I12 (≠ K12), R13 (≠ K13), H16 (≠ F16), Y17 (= Y17), R18 (≠ Q18), D23 (= D23), S24 (= S24), R25 (= R25), K27 (≠ A27), R28 (= R28), R31 (= R31), I33 (= I33), H40 (≠ N40), K42 (≠ I42), S46 (≠ K46), I48 (≠ T50), V61 (≠ Q63), G62 (= G64), Q64 (≠ T66), T66 (≠ S68)
Sites not aligning to the query:
6yw5PP structure of the small subunit of the mitoribosome from Neurospora crassa (see paper)
34% identity, 95% coverage: 1:78/82 of query aligns to 1:86/98 of 6yw5PP
- binding : L2 (≠ V2), K3 (≠ T3), R5 (= R5), L6 (= L6), R8 (= R8), R11 (≠ A11), T12 (≠ K12), N13 (≠ K13), Y17 (= Y17), H23 (≠ D23), A24 (≠ S24), R25 (= R25), A27 (= A27), R28 (= R28), K31 (≠ R31), P41 (= P41), P43 (≠ A43), K56 (vs. gap), V71 (≠ Q63), G72 (= G64), Q74 (≠ T66), T76 (≠ S68), T78 (≠ R70), R81 (≠ A73)
Sites not aligning to the query:
Query Sequence
>GFF4170
MVTIRLARHGAKKRPFYQVVVTDSRNARNGRFIERVGFFNPIASEKEEGTRLDLDRIAHW
VGQGATISDRVAALIKEVKKAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory