SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF6023 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

D3RPC0 Sulfur carrier protein TusA from Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) (Chromatium vinosum) (see paper)
63% identity, 95% coverage: 4:74/75 of query aligns to 5:75/76 of D3RPC0

query
sites
D3RPC0

D

D

R

Q

E

E

L

L

D

D

T

A

R

S

G

G

L

L

N

N

C
|
C

P

P

L

L

P

P

I

I

L

L

K

R

A

A

K

K

S

T

L

L

N

N

E

A

M

M

A

S

S

S

G

G

Q

Q

L

V

L

L

K

H

V

V

V

I

S

A

T

T

D

D

P

P

G

G

S

S

I

V

R

K

D

D

F

F

Q

D

A

A

F

F

A

A

R

K

Q

Q

T

T

G

G

N

N

E

E

L

L

M

M

E

E

Q

S

E

K

T

E

V

E

G

G

T

G

D

K

F

F

I

H

H

F

V

L

L

I

K

K

R

K

C15 (= C14) active site, Cysteine persulfide intermediate; mutation to S: Loss of sulfur binding in the presence of sulfide. Loss of interaction with DsrEFH proteins.

P0A890 Sulfur carrier protein TusA; Sulfur mediator TusA; Sulfur transfer protein TusA; tRNA 2-thiouridine synthesizing protein A from Escherichia coli (strain K12) (see 2 papers)
31% identity, 95% coverage: 4:74/75 of query aligns to 9:79/81 of P0A890

query
sites
P0A890

D

D

R

H

E

T

L

L

D

D

T

A

R

L

G

G

L

L

N

R

C
|
C

P

P

L
x
E

P

P

I

V

L

M

K

M

A

V

K

R

K

K

S

T

L

V

N

R

E

N

M

M

A

Q

S

P

G

G

Q

E

L

T

L

L

K

L

V

I

S

A

T

D

D

D

P

P

G

A

S

T

I

T

R

R

D

D

F

I

Q

P

A

G

F

F

A
x
C

R

T

Q

F

T

M

G

E

N

H

E

E

L

L

M

V

E

A

Q

K

E

E

T

T

V

D

G

G

T

L

D

P

F

Y

I

R

H

Y

V

L

L

I

K

R

R

K

C19 (= C14) active site, Cysteine persulfide intermediate; mutation to S: Unable to restore mnm5s2U formation in a tusA-deficient mutant. Unable to accept sulfur from IscS.
E21 (≠ L16) mutation to K: In sirA1; affects the stability of sigma-S during the logarithmic growth phase.
C56 (≠ A51) mutation to S: Able to restore mnm5s2U formation in a tusA-deficient mutant.

P0A892 Sulfur carrier protein TusA; Sulfur mediator TusA; Sulfur transfer protein TusA; tRNA 2-thiouridine synthesizing protein A from Escherichia coli O157:H7 (see paper)
31% identity, 95% coverage: 4:74/75 of query aligns to 9:79/81 of P0A892

query
sites
P0A892

D

D

R

H

E

T

L

L

D

D

T

A

R

L

G

G

L

L

N

R

C

C

P

P

L
x
E

P

P

I

V

L
x
M

K

M

A

V

K
x
R

K
|
K

S
x
T

L
x
V

N
x
R

E
x
N

M
|
M

A
x
Q

S
x
P

G

G

Q

E

L

T

L

L

K

L

V

I

S

A

T

D

D
|
D

P

P

G

A

S

T

I

T

R
|
R

D
|
D

F

I

Q

P

A

G

F

F

A

C

R

T

Q
x
F

T

M

G

E

N

H

E

E

L

L

M

V

E

A

Q

K

E

E

T

T

V

D

G

G

T

L

D

P

F

Y

I

R

H

Y

V

L

L

I

K

R

R

K

E21 (≠ L16) mutation to A: Decreased binding of IscS.
M24 (≠ L19) mutation to R: No binding of IscS, 50% modified tRNA produced.
R27 (≠ K22) mutation R->D,E: No binding of IscS, 19% modified tRNA produced (for R27E).
27:35 (vs. 22:30, 22% identical) mutation to DKTVRNMQS: No modified tRNA produced.
R31 (≠ N26) mutation to A: No binding of IscS, 30% modified tRNA produced.
D45 (= D40) mutation to A: Binds IscS, 56% modified tRNA produced.
R50 (= R45) mutation to A: Binds IscS, 67% modified tRNA produced.
D51 (= D46) mutation to A: Binds IscS, 67% modified tRNA produced.
F58 (≠ Q53) mutation to A: No binding of IscS, 67% modified tRNA produced.

Query Sequence

>GFF6023
VQVDRELDTRGLNCPLPILKAKKSLNEMASGQLLKVVSTDPGSIRDFQAFARQTGNELME
QETVGTDFIHVLKRR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory