SitesBLAST

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 44% coverage: 122:309/432 of query aligns to 172:353/495 of Q5Q0U0

query
sites
Q5Q0U0

G
|
G

I

L

G

G

E
|
E

S
x
G

G

V

N

T

F
|
F

P
|
P

A

A

G

M

I
x
H

K

A

A

M

V
x
W

T

S

E

S

W

W

F

A

P

P

A

P

K

L

E

E

R

R

A

S

F

K

A

L

I

L

G

T

V

I

F

S

N

Y

A

A

G

G

A

A

N

Q

I

L

G

G

A

T

V

V

I

I

T

S

P

L

L

P

V

L

V

S

P

G

L

I

M

I

V

C

I

Y

A

Y

W

M

G

N

W

W

R

T

M

Y

A

V

F

F

V

Y

V

L

T

F

G

G

V

L

I

V

S

G

F

I

V

V

W

W

L

F

I

I

A

L

W

W

-

M

W

W

A

I

M

V

Y

S

R

D

S

T

P

P

R

E

Q

T

H

H

P

K

K

T

V

I

S

S

E

H

G

Y

E

E

L

K

A

E

W

Y

I

I

E

V

-
x
S

-
x
L

Q
x
K

D
x
N

P

Q

A

L

D

S

P

S

V

Q

K

K

P

V

I

V

G

P

Y

W

A

V

T

S

V

I

L

L

T

K

R

S

K

L

E

P

T

L

W

W

A

A

Y

I

S

V

L

V

G

A

K

H

F

F

F

S

I

Y

D

N

P

W

I

T

W

F

W

Y

F

T

F

L

L

L

F

T

W

L

L

L

P

P

G

T

Y

Y

L

M

F

K

K

E

Q

I

Y

L

D

R

L

F

N

N

L

V

L

Q

T

E

F

N

G

G

I

F

P

L

L

S

A

A

A

L

I
x
P

Y

Y

L

-

I

-

S

-

D

-

V

-

G

-

S

-

V

-

F

F

G

G

G

C

W

W

M

L

S

C

S

-

K

-

L

M

M

I

K

V

A

C

G

G

K

Q

S

A

A

A

N

D

Y

Y

A

L

R

R

G172 (= G122) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E125) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ S126) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (= F129) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P130) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ I133) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ V136) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ ---QD 217:218) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (≠ I280) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
136 K→E: Markedly decreases H(+)-coupled sialic acid transporter activity.
168 R→C: Abolishes H(+)-coupled sialic acid transporter activity.
171 E→C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
371 G→V: Remains in the endoplasmic reticulum.

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
26% identity, 44% coverage: 122:309/432 of query aligns to 172:353/495 of Q8BN82

query
sites
Q8BN82

G

G

I

L

G

G

E

E

S

G

G

V

N

T

F

F

P

P

A

A

G

M

I
x
H

K

A

A

M

V

W

T

S

E

S

W

W

F

A

P

P

A

P

K

L

E

E

R

R

A

S

F

K

A

L

I

L

G

T

V

I

F

S

N

Y

A

A

G

G

A

A

N

Q

I

L

G

G

A

T

V

V

I

I

T

S

P

L

L

P

V

L

V

S

P

G

L

I

M

I

V

C

I

Y

A

Y

W

M

G

N

W

W

R

T

M

Y

A

V

F

F

V

Y

V

L

T

F

G

G

V

I

I

V

S

G

F

I

V

V

W

W

L

F

I

I

A

L

W

W

-

M

W

W

A

I

M

V

Y

S

R

D

S

T

P

P

R

E

Q

T

H

H

P

K

K

T

V

I

S

S

E

H

G

Y

E

E

L

K

A

E

W

Y

I

I

E

V

-

S

-

L

Q

K

D

N

P

Q

A

L

D

S

P

S

V

Q

K

K

P

V

I

V

G

P

Y

W

A

G

T

S

V

I

L

L

T

K

R

S

K

L

E

P

T

L

W

W

A

A

Y

I

S

V

L

V

G

A

K

H

F

F

F

S

I

Y

D

N

P

W

I

S

W

F

W

Y

F

T

F

L

L

L

F

T

W

L

L

L

P

P

G

T

Y

Y

L

M

F

K

K

E

Q

I

Y

L

D

R

L

F

N

N

L

V

L

Q

T

E

F

N

G

G

I

F

P

L

L

S

A

A

A

L

I

P

Y

Y

L

-

I

-

S

-

D

-

V

-

G

-

S

-

V

-

F

F

G

G

G

C

W

W

M

L

S

C

S

-

K

-

L

M

M

I

K

L

A

C

G

G

K

Q

S

A

A

A

N

D

Y

Y

A

L

R

R

H183 (≠ I133) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
24% identity, 78% coverage: 46:383/432 of query aligns to 69:422/493 of Q03567

query
sites
Q03567

K
x
N

P

S

T

S

L

V

M

I

Q

G

D

E

L

F

H

D

W

W

S

D

E

K

R

Q

D

T

F

T

A

G

G

M

V

V

I

L

I

S

W

S

F

F

Q

F

I

Y

A

G

Y

Y

A

I

I

G

G

S

Y

Q

I

I

G

I

F

G

G

G

K

H

F

L

V

A

D

S

M

R

V

Y

G

G

A

G

R

K

V

R

G

V

Y

V

A

F

V

V

A

T

F

I

V

L

I

G

W

S

Q

A

L

L

A

L

H

T

I

L

A

L

H

N

G

P

G

V

A

A

Y

A

S

R

M

T

T

S

Q

E

F

Y

A

A

M

L

A

A

-

I

-

L

R

R

F

A

G

A

L

I

G

G

I

F

G

L

E

Q

S

G

G

A

N

T

F

F

P

P

A

A

G

M

I

H

K

T

A

M

V

W

T

S

E

V

W

W

F

G

P

P

A

P

K

L

E

E

R

L

A

S

F

V

A

L

I

T

G

G

V

V

F

T

N

Y

A

A

G

G

A

A

N

Q

I

I

G

G

A

N

V

V

I

I

T

-

P

-

L

-

V

V

V

L

P

P

L

L

M

S

V

G

I

F

A

L

W

C

-

E

-

Y

-

G

-

F

-

D

-

G

G

G

W

W

R

P

M

S

A

I

F

F

V

Y

V

I

T

I

G

G

V

V

I

F

S

G

F

V

V

L

W

W

L

T

I

A

A

V

W

W

A

Y

M

V

Y

S

R

S

S

D

-

K

P

P

R

A

Q

T

H

H

P

P

K

R

V

I

S

T

E

P

G

E

E

E

L

K

A

Q

W

Y

I

I

-

V

-

T

-

A

-

V

-

E

-

A

-

S

-

M

-

G

E

K

Q

D

D

T

P

G

A

K

D

V

P

P

V

S

K

T

P

P

I

-

G

-

Y

W

A

I

T

K

V

I

L

L

T

T

R

S

K

P

E

A

T

V

W

W

A

A

Y

C

S

W

L

A

G

G

K

H

F

F

F

A

I

G

D

D

P

W

I

G

W

A

W

Y

F

T

F

M

L

L

F

V

W

S

L

L

P

P

G

S

Y

F

L

L

F

K

K

D

Q

V

Y

L

D

G

L

L

N

N

L

L

L

S

T

S

F

L

G

G

-

A

-

V

-

A

-

S

I

I

P

P

L

Y

A

I

A

A

I

Y

Y

F

L

L

I

A

S

I

D

N

V

A

G

G

S

G

V

V

F

L

G

A

G

D

W

T

M

L

S

R

S

S

K

K

L

G

M

I

K

L

A

S

G

T

K

L

S

N

A

T

N

R

Y

R

A

A

R

A

K

M

L

L

T

V

M

A

F

L

V

I

-

G

-

Q

-

G

-

I

-

F

-

L

-

V

-

A

-

S

-

G

-

Y

C

C

A

G

C

C

-

G

-

Q

L

D

V

V

L

L

P

V

V

I

F

F

A

-

E

-

S

-

I

-

T

-

S

-

V

-

W

-

V

-

A

-

V

-

L

I

V

T

I

C

G

G

V

M

A

A

T

I

A

S

G

G

H

L

Q

Q

-

Y

-

A

A

G

F

F

S

V

A

V

N

N

L

Y

Y

L

A

E

L

I

P

A

S

P

D

P

L

F

F

-

P

-

R

-

G

-

A

-

V

S

G

G

S

T

V

V

V

M

G

G

I

T

G

G

G

N

T

T

V

I

G

S

A

A

V

L

G

A

G

G

I

I

L

I

N69 (≠ K46) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
23% identity, 66% coverage: 122:407/432 of query aligns to 172:465/495 of Q9NRA2

query
sites
Q9NRA2

G

G

I

L

G

G

E

E

S

G

G

V

N

T

F

F

P

P

A

A

G

M

I
x
H

K

A

A

M

V

W

T

S

E

S

W

W

F

A

P

P

A

P

K

L

E

E

R

R

A

S

F

K

A
x
L

I
x
L

G

S

V

I

F

S

N

Y

A

A

G

G

A

A

N

Q

I

L

G

G

A

T

V

V

I

I

T

S

P

L

L

P

V

L

V

S

P

G

L

I

M

I

V

C

I

Y

A

Y

W

M

G

N

W

W

R

T

M

Y

A

V

F

F

V

Y

V

F

T

F

G

G

V

T

I

I

S

G

F

I

V

F

W

W

L

F

I

L

A

L

W

W

-

I

W

W

A

L

M

V

Y

S

R

D

S

T

P

P

R

Q

Q

K

H

H

P

K

K

R

V

I

S

S

E

H

G

Y

E

E

L

K

A

E

W

Y

I
|
I

-
x
L

-
x
S

E
x
L

Q
x
R

D
x
N

P

Q

A

L

D

S

P

S

V

Q

K

K

P

S

I

V

G

P

Y

W

A

V

T

P

V

I

L

L

T

K

R

S

K

L

E

P

T

L

W

W

A

A

Y

I

S

V

L

V

G

A

K

H

F

F

F

S

I

Y

D

N

P

W

I

T

W

F

W

Y

F

T

F

L

L

L

F

T

W

L

L

L

P

P

G

T

Y

Y

L

M

F

K

K

E

Q

I

Y

L

D

R

L

F

N

N

L

V

L

Q

T

E

F

N

G
|
G

I

F

P

L

L

S

A

S

A

L

I
x
P

Y

Y

L

L

I

-

S

-

D

-

V

-

G

-

S

-

V

-

F

-

G

G

G

S

W

W

M

L

S

C

S

-

K

-

L

M

M

I

K

L

A

S

G

G

K

Q

S

A

A

A

N

D

Y

N

A

L

R

R

K

A

L

K

T

W

M

N

F

F

V

S

C

T

A

L

C

C

-

V

-

R

-

I

-

F

-

S

-

L

-

I

-
x
G

L

M

V

I

L

G

P

P

V

A

V

V

F

F

A

L

E

V

S

A

I

A

-

G

-

F

-

I

-

G

-

C

-

D

T

Y

S

S

V

L

W

A

V

V

A

A

V

F

L

L

V

T

I

I

G

S

V

T

A

T

T

L

A

G

G

G

-

F

-

C

H

S

Q

S

A

G

F

F

S

S

A

I

N

N

L

H

Y

L

A

-

L

-

P

-

S

-

D

D

L

I

F

A

P

P

R

S

G

Y

A

A

V

-

G

G

S

I

V

L

G

G

I

I

G

T

G

N

T

T

V

F

G

A

A

T

V

I

G

P

G

G

I

M

L

V

M

G

A

P

F

V

Y

I

A

A

G

K

E

S

I

L

L

T

-

P

-

D

D

N

R

T

L

V

G

G

V

E

Y

W

W

Q

P

T

L

V

F

F

W

Y

V

I

A

A

G

A

S

A

H183 (≠ I133) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AI 148:149) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ I- 215) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ --EQD 216:218) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G274) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (≠ I280) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (vs. gap) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
136 K → E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795

8u3hA Structure of fmoc-leu-oh bound sialin
24% identity, 46% coverage: 122:319/432 of query aligns to 109:300/425 of 8u3hA

query
sites
8u3hA

G

G

I

L

G

G

E

E

S

G

G

V

N

T

F

F

P

P

A

A

G

M

I

H

K

A

A

M

V

W

T

S

E

S

W

W

F

A

P

P

A

P

K

L

E

E

R

R

A

S

F

K

A

L

I

L

G

S

V

I

F

S

N

Y

A

A

G

G

A

A

N

Q

I

L

G

G

A

T

V

V

I

I

T

S

P

L

L

P

V

L

V

S

P

G

L

I

M

I

V

C

I

Y

A

Y

W

M

G

N

W

W

R

T

M

Y

A

V

F

F

V

Y

V

F

T

F

G

G

V

T

I

I

S

G

F

I

V

F

W

W

L

F

I

L

A

L

W

W

-

I

W

W

A

L

M

V

Y

S

R

D

S

T

P

P

R

Q

Q

K

H

H

P

K

K

R

V

I

S

S

E

H

G

Y

E

E

L

K

A

E

W

Y

I

I

-

L

-

S

E

L

Q

R

D

N

P

Q

A

L

D

S

P

S

V

Q

K

K

P

S

I

V

G

P

Y

W

A

V

T

P

V

I

L

L

T

K

R

S

K

L

E

P

T

L

W

W

A

A

Y

I

S

V

L

V

G

A

K
x
H

F

F

F

S

I
x
Y

D

N

P

W

I

T

W

F

W
x
Y

F

T

F

L

L

L

F

T

W

L

L

L

P

P

G

T

Y

Y

L

M

F

K

K

E

Q

I

Y

L

D

R

L

F

N

N

L

V

L

Q

T

E

F

N

G

G

I

F

P

L

L

S

A

S

A

L

I

P

Y

Y

L

L

I

-

S

-

D

-

V

-

G

-

S

-

V

-

F

-

G

G

G

S

W

W

M

L

S

C

S

-

K

-

L
x
M

M

I

K

L

A

S

G

G

K

Q

S

A

A

A

N

D

Y

N

A

L

R

R

K

A

L

K

T

W

M

N

F

F

V

S

C

T

A

L

C

C

L

V

binding leucine: M279 (≠ L298)
binding Fluorenylmethyloxycarbonyl chloride: H235 (≠ K244), Y238 (≠ I247), Y243 (≠ W252)

Sites not aligning to the query:

8u3gA Structure of naag-bound sialin
24% identity, 46% coverage: 122:319/432 of query aligns to 111:302/427 of 8u3gA

query
sites
8u3gA

G

G

I

L

G

G

E

E

S

G

G

V

N

T

F
|
F

P

P

A

A

G

M

I

H

K

A

A

M

V

W

T

S

E

S

W

W

F

A

P

P

A

P

K

L

E

E

R

R

A

S

F

K

A

L

I

L

G

S

V

I

F

S

N

Y

A

A

G

G

A

A

N

Q

I

L

G

G

A

T

V

V

I

I

T

S

P

L

L

P

V

L

V

S

P

G

L

I

M

I

V

C

I

Y

A

Y

W

M

G

N

W

W

R

T

M

Y

A

V

F

F

V

Y

V

F

T

F

G

G

V

T

I

I

S

G

F

I

V

F

W

W

L

F

I

L

A

L

W

W

-

I

W

W

A

L

M

V

Y

S

R

D

S

T

P

P

R

Q

Q

K

H

H

P

K

K

R

V

I

S

S

E

H

G

Y

E

E

L

K

A

E

W

Y

I

I

-

L

-

S

E

L

Q

R

D

N

P

Q

A

L

D

S

P

S

V

Q

K

K

P

S

I

V

G

P

Y

W

A

V

T

P

V

I

L

L

T

K

R

S

K

L

E

P

T

L

W

W

A

A

Y

I

S

V

L

V

G

A

K

H

F

F

F

S

I

Y

D

N

P

W

I

T

W

F

W

Y

F

T

F

L

L

L

F

T

W

L

L

L

P

P

G

T

Y

Y

L

M

F

K

K

E

Q

I

Y

L

D

R

L

F

N

N

L

V

L

Q

T

E

F

N

G

G

I

F

P

L

L

S

A

S

A

L

I

P

Y

Y

L

L

I

-

S

-

D

-

V

-

G

-

S

-

V

-

F

-

G

G

G

S

W

W

M

L

S

C

S

-

K

-

L

M

M

I

K

L

A

S

G

G

K

Q

S

A

A

A

N

D

Y

N

A

L

R

R

K

A

L

K

T

W

M

N

F

F

V

S

C

T

A

L

C

C

L

V

binding aspartic acid: F118 (= F129)

Sites not aligning to the query:

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 63% coverage: 118:389/432 of query aligns to 184:465/582 of Q9JI12

query
sites
Q9JI12

R
|
R

F

I

G

L

L

Q

G

G

I

L

G

V

E
|
E

S

G

G

V

N

T

F

Y

P

P

A

A

G

C

I

H

K

G

A

I

V

W

T

S

E

K

W

W

F

A

P

P

A

P

K

L

E

E

R

R

A

S

F

R

A

L

I

A

G

T

V

T

F

S

N

F

A

C

G

G

A

S

N

Y

I

A

G

G

A

A

V

V

I

I

T

A

P

M

L

P

V

L

V

A

P

G

L

I

M

L

V

V

I

Q

A

Y

W

T

G

G

W

W

R

S

M

S

A

V

F

F

V

Y

V

V

T

Y

G

G

V

S

I

F

S

G

F

M

V

V

W

W

L

Y

I

M

A

F

W

W

W

L

A

L

M

V

-

S

Y

Y

R

E

S

S

P

P

R

A

Q

K

H

H

P

P

K

T

V

I

S

T

E

D

G

E

E

E

L

R

A

R

W

Y

I

I

E

E

Q

E

D

S

P

I

A

G

D

E

P

S

V

-

K

-

P

-

I

-

G

-

Y

-

A

A

T

N

V

L

L

L

T

G

R

A

K

M

E

E

T

K

W

F

A

K

Y

T

S

P

L

W

G

R

K

K

F

F

I

T

D

S

-

M

P

P

I

V

-

Y

-

A

-

I

-

V

-

A

-

N

-

F

-

C

-
x
R

-

S

W

W

W

T

F

F

F

Y

L

L

F

L

W

L

L

I

-

S

-

Q

P

P

G

A

Y

Y

L

F

F

E

K

E

Q

V

Y

F

D

G

L

F

N

E

L

I

L

S

T

K

F

V

G

G

-

M

-

L

-

S

-

A

I

V

P

P

L

H

A

L

A

V

I

M

Y

T

L

I

I

I

S

V

D

P

V

I

G

G

S

G

V

Q

F

I

G

A

G

D

W

F

M

L

S

R

S

S

K

K

L

Q

M

I

K

L

A

S

G

T

K

T

S

T

A

V

N

R

Y

K

A

I

R

M

K

N

L

C

T

G

M

G

F

F

V

G

C

M

A

E

C

A

L

T

V

L

L

L

P

L

V

V

F

G

A

Y

E

S

S

H

I

T

T

R

S

G

V

V

W

A

V

I

A

S

V

F

L

L

V

V

I

L

G

A

V

V

A

G

T

F

A

S

G

G

H

F

Q

A

A

I

F

S

S

G

A

F

N

N

L

V

Y

N

A

H

L

L

P

-

S

-

D

D

L

I

F

A

P

P

R

R

G

Y

A

A

V

-

G

S

S

I

V

L

V

M

G

G

I

I

G

S

G

N

T

G

V

V

G

G

A

T

V

L

G

S

G

G

I

M

L

V

M

C

A

P

F

I

Y

I

A

V

G

G

R184 (= R118) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E125) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (vs. gap) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
128 H→A: Greatly lowers L-glutamate transport.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
22% identity, 63% coverage: 122:394/432 of query aligns to 130:412/452 of 7t3nA

query
sites
7t3nA

G

G

I

L

G

V

E

Q

S

G

G

V

N

T

F
x
Y

P

P

A

A

G

C

I

H

K

G

A

I

V

W

T

S

E

K

W

W

F

A

P

P

A

P

K

L

E
|
E

R

R

A

S

F

R

A

L

I

A

G

T

V

T

F

S

N

F

A

C

G
|
G

A

S

N
x
Y

I

A

G

G

A

A

V

V

I

I

T

A

P

M

L

P

V

L

V

A

P

G

L

I

M

L

V

V

I

Q

A

Y

W

T

G

G

W

W

R

S

M

S

A

V

F

F

V

Y

V

V

T

Y

G

G

V

S

I

F

S

G

F

M

V

V

W

W

L

Y

I

M

A

F

W

W

W

L

A

L

M

V

-

S

Y

Y

R

E

S

S

P

P

R

A

Q

K

H

H

P

P

K

T

V

I

S

T

E

D

G

E

E

E

L

R

A

R

W

Y

I

I

E

E

Q

E

D

S

P

I

A

G

D

E

P

S

V

A

K

N

P

L

I

L

G

G

-

A

-

M

-

E

-

K

-

F

-

K

-

T

-

P

Y

W

A

R

T

K

V

F

L

F

T

T

R

S

K

M

E

P

T

V

W

Y

A

A

Y

I

S

I

L

V

G

A

K

N

F

F

F

C

I
x
R

D

S

P

W

I

T

W
x
F

W
x
Y

F

L

L

L

F

I

W

S

L

Q

P

P

G

A

Y

Y

L

F

F

E

K

E

Q

V

Y

F

D

G

L

F

N

E

L

I

L

S

T

K

F

V

G

G

-

M

-

L

-

S

-

A

I

V

P

P

L

H

A

L

A

V

I

M

Y

T

L

I

I

I

S

V

D

P

V

I

G

G

S

G

V

Q

F

I

G

A

G

D

W

F

M

L

S

R

S

S

K

K

L

Q

M

I

K

L

A

S

G

T

K

T

S

T

A

V

N

R

Y

K

A

I

R

M

K

N

L

C

T

G

M

G

F

F

V

G

C

M

A

E

C

A

L

T

V

L

L

L

P

L

V

V

F

G

A

Y

E

S

S

H

I

T

T

R

S

G

V

V

W

A

V

I

A

S

V

F

L

L

V

V

I

L

G

A

V

V

A

G

T

F

A

S

G

G

H

F

Q

A

A

I

F

S

S

G

A

F

N

N

L

V

Y

N

A

H

L

L

P

-

S

-

D

D

L

I

F

A

P

P

R

R

G

Y

A

A

V

-

G

S

S

I

V

L

V

M

G

G

I

I

G

S

G

N

T

G

V

V

G

G

A

T

V

L

G

S

G

G

I

M

L

V

M

C

A

P

F

I

Y

I

A

V

G

G

E

A

I

M

L

T

D

K

R

N

binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y137 (≠ F129), Y165 (≠ N157), R264 (≠ I247), F268 (≠ W251), Y269 (≠ W252)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E144), G163 (= G155)

Sites not aligning to the query:

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
23% identity, 95% coverage: 14:425/432 of query aligns to 13:441/452 of Q5EXK5

query
sites
Q5EXK5

A

A

N

P

A

V

G

G

R

A

Y

R

R

Q

W

W

M

R

I

V

V

I

A

I

L

C

F

F

A

L

A

V

T

V

V

M

I

L

N

D

Y

G

I

F

D

D

R

T

Q

A

M

A

I

I

G

G

V

F

L

I

K

A

P

P

T

D

L

I

M

R

Q

S

D

H

L

W

H

Q

W

L

S

T

E

A

R

G

D

D

F

L

A

A

G

P

V

L

I

F

I

G

W

A

F

G

Q

L

I

L

A

G

Y

L

A

T

I

A

G

G

Y

A

I

L

G

L

F

C

G

G

K

P

F

L

V

S

D
|
D

M

R

V

F

G

G

A

R

R

K

V

R

G

V

Y

I

A

E

V

F

A

C

F

V

V

A

I

L

W

F

Q

G

L

L

A

F

H

S

I

L

A

L

H

S

G

A

G

F

A

A

Y

P

S

D

M

L

T

Q

Q

M

F

L

A

V

M

F

A

L

R

R

F

F

G

L

L

T

G

G

I

L

G

G

E

L

S

G

G

G

N

A

F

M

P

P

A

N

G

T

I

I

K

T

A

M

V

T

T

S

E

E

W

Y

F

L

P

P

A

A

K

R

E

R

R

R

A

G

F

A

A

L

I

V

G

T

V

L

-

M

-

F

-

C

-

G

F

F

N

T

A

L

G

G

A

S

N

A

I

F

G

G

A

G

V

V

I

V

T

S

P

A

L

Q

V

L

V

V

P

P

L

L

M

I

V

-

I

-

A

-

W

-

G

G

W

W

R

H

M

G

A

I

F

L

V

V

V

L

T

G

G

G

V

V

I

L

S

P

F

L

V

I

W

L

L

A

I

V

A

A

W

L

W

V

A

P

M

L

Y

L

-

P

R

E

S

S

P

P

R

R

-

W

-

Q

-

I

-

R

Q

Q

H

L

P

P

K

Q

V

A

S

T

-

I

-

A

-

R

-

T

-

V

-

G

-

A

-

I

E

T

G

G

E

E

L

R

A

Y

-

N

-

D

-

T

-

H

-

F

W

W

I

L

E

D

Q

-

D

E

P

P

A

A

D

A

P

G

V

A

K

K

P

-

I

-

G

G

Y

S

A

I

T

S

V

Q

L

L

T

F

R

A

K

G

E

R

T

Q

W

L

A

A

Y

I

S

T

L

L

G

M

K

L

F

W

F

V

I

V

D

-

P

-

I

-

W

-

F

F

L

M

F

S

W

L

L

L

P

I

G

I

Y

Y

L

L

F

L

K

S

Q

S

Y

W

D

M

L

P

N

T

L

L

T

N

F

H

-

R

G

G

I

I

P

D

L

L

A

Q

A

H

I

A

Y

S

L

W

I

V

S

T

-

A

-

F

D

Q

V

I

G

G

S

G

V

T

F

F

G

G

G

A

W

L

M

L

S

L

S

G

K
x
V

L

L

M

M

K
x
D

A

-

G

-

K

-

S

R

A

F

N

N

Y

P

A

Y

R

R

K

V

L

L

T

A

M

L

F

S

V

Y

C

G

A

L

C

G

L

A

V

V

L

C

P

I

V

V

V

M

F

I

A

G

E

L

S

S

I

E

T

N

S

G

V

L

W

W

V

L

A

M

V

A

L

L

V

A

I

I

-

F

-

G

-

T

G

G

V

I

A

G

T

I

A

S

G

G

H

S

Q

Q

A

V

F

-

S

-

A

-

N

G

L

L

Y

N

A

A

L

L

P

T

S

A

D

T

L

L

F

Y

P

P

R

T

G

Q

A

S

V

R

G

A

S

T

V

G

V

V

G

S

I

W

G

S

G

N

T

A

V

V

G

G

A

R

V

C

G

G

G

A

I

I

L

V

M

G

A

S

F

L

Y

S

A

G

G

G

E

V

I

M

L

M

D

A

R

M

L

N

G

F

V

S

Y

F

W

D

P

T

L

L

F

F

W

F

V

V

A

I

G

A

-

V

-

P

-

A

-

V

S

S

A

A

Y

V

F

M

V

L

A

I

L

L

V

T

V

L

H

A

L

V

L

R

S

N

P

P

R

T

L

S

E

V

P

P

D82 (= D83) mutation to A: Loss of activity.
V311 (≠ K297) mutation to W: Loss of activity.
D314 (≠ K300) mutation to A: Loss of activity.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
23% identity, 38% coverage: 19:184/432 of query aligns to 25:190/448 of Q51955

query
sites
Q51955

R

R

Y

Y

R

Q

W

W

M

R

I

V

V

V

A

L

L

L

L

C

F

F

A

L

A

I

T

V

V

F

I

L

N
x
D

Y

G

I

L

D
|
D

R

T

Q

A

M

A

I

M

G

G

V

F

L

I

K

A

P

P

T

A

L

L

M

S

Q

Q

D

E

L

W

H

G

W

I

S

D

E

R

R

A

D

S

F

L

A

G

G

P

V

V

I

M

I

S

W

A

F

A

Q

L

I

I

A

G

Y

M

A

V

I

F

G

G

Y

A

I

L

G

G

F

S

G
|
G

K

P

F

L

V

A

D
|
D

M

R

V

F

G
|
G

A

R

R

K

V

G

G

V

Y

L

A

V

V

G

A

A

F

V

V

L

I

V

W

F

Q

G

L

G

A

F

H

S

I

L

A

A

H

S

G

A

G

Y

A

A

Y

T

S

N

M

V

T

D

Q

Q

F

L

A

L

M

V

A

L

R
|
R

F

F

G

L

L

T

G

G

I

L

G

G

E

L

S

G

G

A

N

G

F

M

P

P

A

N

G

A

I

T

K

T

A

L

V

L

T

S

E
|
E

W

Y

F

T

P

P

A

E

K

R

E

L

R

K

A

S

F

L

A

L

I

V

G

T

V

S

F

M

N

F

A

C

G

G

A

F

N

N

I

L

G

G

A

M

V

A

I

G

T

G

P

G

L

F

V

I

V

S

P

A

L

K

M

M

V

I

I

P

A

A

W

Y

G

G

W

W

R
x
H

M

S

A

L

F

L

V

V

V

I

T

G

G

G

D41 (≠ N35) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D38) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (= G79) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (= D83) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G86) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R118) mutation to A: Abolishes 4-HBA transport.
E144 (= E138) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ R177) mutation to A: Decrease in 4-HBA transport and chemotaxis.

Sites not aligning to the query:

323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
24% identity, 38% coverage: 138:300/432 of query aligns to 190:357/497 of Q9Y2C5

query
sites
Q9Y2C5

E

K

W

W

F

A

P

P

A

P

K

L

E

E

R

R

A

S

F

Q

A

L

I

T

G

T

V

I

F

A

N

G

A

S

G

G

A

S

N

M

I

L

G

G

A

S

V

F

I

I

T

V

P

L

L

L

V

A

V

G

P

G

L

L

M

L

V

C

I

Q

A

T

W

I

G

G

W

W

R

P

M

Y

A

V

F

F

V

Y

V

I

T

F

G

G

V

G

I

I

S

G

F

C

V

A

W

C

L

C

I

P

A

L

W

W

W

F

A

P

M

L

-

I

Y

Y

R

D

S

D

P

P

R

V

Q

N

H

H

P

P

K

F

V

I

S

S

E

A

G

G

E

E

L

K

A

R

W

Y

I

I

-

V

-

C

-

S

-

L

E

A

Q

Q

D

Q

P

D

A

C

D

S

P

P

V

G

K

W

P

S

I

L

G

P

Y

I

A

R

T

A

V

M

L

I

T

K

R

S

K

L

E

P

T

L

W

W

A

A

Y

I

S

-

L

L

G

V

K

S

F

Y

F

F

I

C

D

E

P

-

I

-

W

Y

W

W

F

L

F

F

-

Y

-

T

-

I

L

M

F

A

W

Y

L

T

P

P

G

T

Y

Y

L

I

F

S

K

S

Q

V

Y

L

D

Q

L

A

N

N

L

L

L

R

T

D

F

S

G

G

I

I

P

L

L

S

A

A

A

L

I

P

Y

F

L

V

I

V

S

G

D

C

V

I

G

C

S

I

V

I

F

L

G

G

W

L

M

L

S

A

S

D

K

F

L

L

M

L

K

S

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
372 A → T: in dbSNP:rs11754288

Query Sequence

>Ga0059261_2977 FitnessBrowser__Korea:Ga0059261_2977
MDASQAEGIRTGAAANAGRYRWMIVALLFAATVINYIDRQMIGVLKPTLMQDLHWSERDF
AGVIIWFQIAYAIGYIGFGKFVDMVGARVGYAVAFVIWQLAHIAHGGAYSMTQFAMARFG
LGIGESGNFPAGIKAVTEWFPAKERAFAIGVFNAGANIGAVITPLVVPLMVIAWGWRMAF
VVTGVISFVWLIAWWAMYRSPRQHPKVSEGELAWIEQDPADPVKPIGYATVLTRKETWAY
SLGKFFIDPIWWFFLFWLPGYLFKQYDLNLLTFGIPLAAIYLISDVGSVFGGWMSSKLMK
AGKSANYARKLTMFVCACLVLPVVFAESITSVWVAVLVIGVATAGHQAFSANLYALPSDL
FPRGAVGSVVGIGGTVGAVGGILMAFYAGEILDRLGVYWPLFWVAGSAYFVALLVVHLLS
PRLEPVKVDETA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory