SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_3663 Ga0059261_3663 UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8Z9S7 Bifunctional protein GlmU; EC 2.7.7.23; EC 2.3.1.157 from Yersinia pestis
42% identity, 97% coverage: 1:438/451 of query aligns to 1:451/456 of Q8Z9S7

query
sites
Q8Z9S7

M

M

T

S

T

N

S

S

P

S

I

M

A

S

A

V

V

V

I

I

L

L

A

A

G

G

S

K

G

G

T

T

R

R

M

M

K

Y

S

S

D

D

L

L

H

P

K

K

V

V

L

L

H

H

P

P

I

L

A

A

G

G

R

K

P

P

M

M

L

V

L

Q

H

H

L

V

I

I

D

D

T

A

V

A

A

M

S

K

L

L

S

G

P

A

E

Q

R

H

A

V

V

H

V

L

V

V

V

Y

G

G

A

H

R

G

E

E

Q

L

V

L

E

K

A

K

A

T

V

L

A

A

P

D

H

P

G

S

V

L

A

N

S

W

A

V

H

L

Q

Q

A

A

E

E

Q

Q

L

L

G

G

T

T

G

G

H

H

A

A

V

M

M

Q

Q

Q

A

A

R

A

E

P

A

H

L

F

A

A

G

D

F

-

D

D

G

E

D

D

V

I

L

L

V

M

L

L

Y

Y

G

G

D

D

V

V

P

P

L

L

V

I

T

S

E

V

E

D

T

T

M

L

R

Q

R

R

M

L

V

L

G

A

R

A

L

K

H

P

E

E

S

G

D

-

A

-

P

-

S

G

V

I

V

G

V

L

L

L

G

T

F

V

R

K

P

L

A

D

D

N

A

P

A

S

A

G

Y

Y

G

G

R

R

V

I

I

V

V

R

D

E

G

N

G

G

G

-

R

D

I

V

E

V

K

G

I

I

V

V

E

E

Y

H

K

K

D

D

A

A

S

S

P

D

E

A

E

Q

R

R

A

E

V

I

T

N

L

E

C

I

N

N

S

T

G

G

L

I

M

L

A

V

V

A

R

N

S

G

A

R

D

D

L

L

F

K

A

R

L

W

L

L

D

S

R

L

L

L

G

D

N

N

D

N

N

N

A

A

A

Q

S

G

E

E

Y

F

Y

Y

L

I

T

T

D

D

I

I

V

I

E

A

L

L

A

A

N

H

A

A

H

D

V

G

R

K

G

K

A

I

A

A

V

T

I

V

E

H

-

P

T

T

D

R

A

L

A

S

E

E

V

V

A

E

G

G

V

V

N

N

S

N

R

R

S

L

D

Q

L

L

A

S

A

A

V

L

E

E

A

R

A

V

W

F

Q

Q

Q

T

V

E

R

Q

R

A

A

E

R

K

A

L

M

L

A

L

E

A

G

G

V

V

T

M

L

L

T

L

A

D

P

P

E

S

T

R

V

F

W

D

F

L

A

R

Y

G

D

E

T

L

V

T

L

H

G

G

R

R

D

D

V

I

T

T

V

I

E

D

P

T

N

N

V

V

V

I

F

I

-

E

-

G

-

H

-

V

-

I

-

L

-

G

-

D

-

R

-

V

-

R

-

I

G

G

P

T

G

G

V

C

T

V

-

L

-

K

-

N

-

C

-

V

V

I

A

G

D

D

V

S

N

E

I

I

R

S

A

P

F

Y

S

T

H

V

I

L

E

E

G

D

A

A

S

R

I

L

A

D

T

A

G

N

A

C

E

T

I

V

G

G

P

P

Y

F

A

A

R

R

L

L

R
|
R

P

P

G

G

T

A

V

E

V

L

G

A

E

E

K

G

A

A

K

H

I

V

G

G

N

N

F

F

V

V

E

E

T

I

K
|
K

K

K

T

A

V

R

L

L

G

G

K

K

G

G

A

S

K

K

A

A

N

G

H

H

L

L

T

S

Y
|
Y

L

L

G

G

D

D

A

A

E

E

V

I

G

G

A

A

G

G

A

V

N
|
N

I

I

G

G

A

A

G

G

T

T

I

I

T

T

C

C

N

N

Y

Y

D

D

G

G

F

A

F

N

K

K

Y

F

G

K

T

T

R

I

I

I

G

G

A

D

G

D

A

V

F

F

I

V

G

G

S

S

N

D

S

T

A

Q

L

L

V

V

A

A

P

P

V

V

A

T

I

V

G

A

E

N

G

G

A

A

I

T

V

I

A

G

A

A

G

G

A

T

V

T

V

V

T

T

K

R

D

D

V

V

E

A

A

E

D

N

A

E

L

L

A

V

L

I

V

S

R

R

P

V

E

K

Q

Q

V

V

A

H

K

I

P

Q

G

G

W

W

A

K

K

R

R333 (= R320) binding
K351 (= K338) binding
Y366 (= Y353) binding
N377 (= N364) binding

P0ACC7 Bifunctional protein GlmU; EC 2.7.7.23; EC 2.3.1.157 from Escherichia coli (strain K12) (see 6 papers)
42% identity, 97% coverage: 1:438/451 of query aligns to 1:451/456 of P0ACC7

query
sites
P0ACC7

M
|
M

T
x
L

T
x
N

S
x
N

P
x
A

I
x
M

A
x
S

A
x
V

V
|
V

I
|
I

L
|
L

A
|
A

G
|
G

S
x
K

G
|
G

T
|
T

R
|
R

M
|
M

K
x
Y

S
|
S

D
|
D

L
|
L

H
x
P

K
|
K

V
|
V

L
|
L

H
|
H

P
x
T

I
x
L

A
|
A

G
|
G

R
x
K

P
x
A

M
|
M

L
x
V

L
x
Q

H
|
H

L
x
V

I
|
I

D
|
D

T
x
A

V
x
A

A
x
N

S
x
E

L
|
L

S
x
G

P
x
A

E
x
A

R
x
H

A
x
V

V
x
H

V
x
L

V
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V

V
x
Y

G
|
G

A
x
H

R
x
G

E
x
D

Q
x
L

V
x
L

E
x
K

A
x
Q

A
|
A

V
x
L

A
x
K

P
x
D

H
x
D

G
x
N

V
x
L

A
x
N

S
x
W

A
x
V

H
x
L

Q
|
Q

A
|
A

E
|
E

Q
|
Q

L
|
L

G
|
G

T
|
T

G
|
G

H
|
H

A
|
A

V
x
M

M
x
Q

Q
|
Q

A
|
A

R
x
A

E
x
P

A
x
F

L
x
F

A
|
A

G
x
D

F

-

D
|
D

G
x
E

D
|
D

V
x
I

L
|
L

V
x
M

L
|
L

Y
|
Y

G
|
G

D
|
D

V
|
V

P
|
P

L
|
L

V
x
I

T
x
S

E
x
V

E
|
E

T
|
T

M
x
L

R
x
Q

R
|
R

M
x
L

V

-

G

-

R

-

L

-

H

-

E

-

S
x
R

D
|
D

A
|
A

P
x
K

-
x
P

-
x
Q

-
x
G

S
x
G

V
x
I

V
x
G

V
x
L

L
|
L

G
x
T

F
x
V

R
x
K

P
x
L

A
x
D

D
|
D

A
x
P

A
x
T

A
x
G

Y
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Y

G
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G

R
|
R

V
x
I

I
x
T

V
x
R

D
x
E

G
x
N

G
|
G

G

-

R
x
K

I
x
V

E
x
T

K
x
G

I
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I

V
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V

E
|
E

Y
x
H

K
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K

D
|
D

A
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A

S
x
T

P
x
D

E
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E

E
x
Q

R
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R

A
x
Q

V
x
I

T
x
Q

L
x
E

C
x
I

N
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N

S
x
T

G
|
G

L
x
I

M
x
L

A
x
I

V
x
A

R
x
N

S
x
G

A
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A

D
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D

L
x
M

F
x
K

A
x
R

L
x
W

L
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L

D
x
A

R
x
K

L
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L

G
x
T

N
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N

D
x
N

N
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N

A
|
A

A
x
Q

S
x
G

E
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E

Y
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Y

L
x
I

T
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T

D
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D

I
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I

V
x
I

E
x
A

L
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L

A
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A

N
x
Y

A
x
Q

H
x
E

V
x
G

R
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R

G
x
E

-
x
I

A
x
V

A
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A

V
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V

I
x
H

E
x
P

T
x
Q

D
x
R

A
x
L

A
x
S

E
|
E

V
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V

A
x
E

G
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G

V
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V

N
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N

S
x
N

R
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R

S
x
L

D
x
Q

L
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L

A
x
S

A
x
R

V
x
L

E
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E

A
x
R

A
x
V

W
x
Y

Q
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Q

Q
x
S

V
x
E

R
x
Q

R
x
A

A
x
E

R
x
K

A
x
L

M
x
L

A
x
L

E
x
A

G
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G

V
|
V

T
x
M

L
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L

T
x
R

A
x
D

P
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P

E
x
A

T
x
R

V
x
F

W
x
D

F
x
L

A
x
R

Y
x
G

D
x
T

T
x
L

V
x
T

L
x
H

G
|
G

R
|
R

D
|
D

V
|
V

T
x
E

V
x
I

E
x
D

P
x
T

N
|
N

V
|
V

V
x
I

F
x
I

G
x
E

P
x
G

G
x
N

V
|
V

T
|
T

-
x
L

-
x
G

-
x
H

-
x
R

-
x
V

-
x
K

-
x
I

-
x
G

-
x
T

-
x
G

-
x
C

-
x
V

-
x
I

-
x
K

-
x
N

-
x
S

-
x
V

V
x
I

A
x
G

D
|
D

V
x
C

N
x
E

I
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I

R
x
S

A
x
P

F
x
Y

S
x
T

H
x
V

I
x
V

E
|
E

G
x
D

A
|
A

S
x
N

I
x
L

A
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A

T
x
A

G
x
A

A
x
C

E
x
T

I
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I

G
|
G

P
|
P

Y
x
F

A
|
A

R
|
R

L
|
L

R
|
R

P
|
P

G
|
G

T
x
A

V
x
E

V
x
L

G
x
L

E
|
E

K
x
G

A
|
A

K
x
H

I
x
V

G
|
G

N
|
N

F
|
F

V
|
V

E
|
E

T
x
M

K
|
K

T
x
A

V
x
R

L
|
L

G
|
G

K
|
K

G
|
G

A
x
S

K
|
K

A
|
A

N
x
G

H
|
H

L
|
L

T
|
T

Y
|
Y

L
|
L

G
|
G

D
|
D

A
|
A

E
|
E

V
x
I

G
|
G

A
x
D

G
x
N

A
x
V

N
|
N

I
|
I

G
|
G

A
|
A

G
|
G

T
|
T

I
|
I

T
|
T

C
|
C

N
|
N

Y
|
Y

D
|
D

G
|
G

F
x
A

F
x
N

K
|
K

Y
x
F

G
x
K

T
|
T

R
x
I

I
|
I

G
|
G

A
x
D

G
x
D

A
x
V

F
|
F

I
x
V

G
|
G

S
|
S

N
x
D

S
x
T

A
x
Q

L
|
L

V
|
V

A
|
A

P
|
P

V
|
V

A
x
T

I
x
V

G
|
G

E
x
K

G
|
G

A
|
A

I
x
T

V
x
I

A
|
A

G
|
G

A
x
T

V
x
T

V
|
V

T
|
T

K
x
R

D
x
N

V
|
V

E
x
G

A
x
E

D
x
N

A
|
A

L
|
L

A
|
A

L
x
I

V
x
S

R
|
R

P
x
V

E
x
P

Q
|
Q

V
x
T

A
x
Q

K
|
K

P
x
E

G
|
G

W
|
W

A
x
R

K
x
R

1:229 (vs. 1:233, 41% identical) Pyrophosphorylase
LAAG 11:14 (= LAAG 11:14) binding
G14 (= G14) mutation to A: 8-fold decrease in uridyltransferase activity. Creates steric conflict and decreases affinity for UTP.
R18 (= R18) mutation to A: Dramatically impairs the uridyltransferase activity.
K25 (= K25) mutation to A: 8-fold decrease in uridyltransferase activity.
Q76 (= Q76) binding
GT 81:82 (= GT 81:82) binding
YGD 103:105 (= YGD 104:106) binding
D105 (= D106) binding ; binding
G140 (= G144) binding
E154 (= E159) binding
N169 (= N174) binding
N227 (= N231) binding ; binding
230:250 (vs. 234:254, 14% identical) Linker
C296 (vs. gap) mutation to A: No effect.
C307 (≠ V294) mutation to A: 1350-fold decrease in acetyltransferase activity.
C324 (≠ A311) mutation to A: 8-fold decrease in acetyltransferase activity.
R333 (= R320) binding
K351 (= K338) binding
Y366 (= Y353) binding
N377 (= N364) binding
A380 (= A367) binding
C385 (= C372) mutation to A: No effect.
S405 (= S392) binding
A423 (= A410) binding
R440 (= R427) binding

Sites not aligning to the query:

251:456 N-acetyltransferase

2oi6B E. Coli glmu- complex with udp-glcnac, coa and glcn-1-po4 (see paper)
42% identity, 97% coverage: 3:438/451 of query aligns to 1:449/452 of 2oi6B

query
sites
2oi6B

T

N

S

N

P

A

I

M

A

S

A

V

V

V

I

I

L
|
L

A
|
A

G
|
G

S

K

G

G

T

T

R
|
R

M

M

K

Y

S

S

D

D

L

L

H

P

K
|
K

V

V

L

L

H

H

P

T

I

L

A

A

G

G

R

K

P

A

M

M

L

V

L

Q

H

H

L

V

I

I

D

D

T

A

V

A

A

N

S

E

L

L

S

G

P

A

E

A

R

H

A

V

V

H

V

L

V

V

V

Y

G

G

A

H

R

G

E

D

Q

L

V

L

E

K

A

Q

A

A

V

L

A

K

P

D

H

D

G

N

V

L

A

N

S

W

A

V

H

L

Q
|
Q

A

A

E

E

Q
|
Q

L
|
L

G
|
G

T
|
T

G

G

H

H

A
|
A

V

M

M

Q

Q

Q

A

A

R

A

E

P

A

F

L

F

A

A

G

D

F

-

D

D

G

E

D

D

V

I

L

L

V

M

L

L

Y
|
Y

G
|
G

D
|
D

V

V

P

P

L

L

V

I

T

S

E

V

E

E

T

T

M

L

R

Q

R

R

M

L

V

-

G

-

R

-

L

-

H

-

E

-

S

R

D

D

A

A

P

K

-

P

-

Q

-

G

S

G

V

I

V

G

V

L

L

L

G

T

F

V

R

K

P

L

A

D

D

D

A

P

A

T

A

G

Y
|
Y

G
|
G

R

R

V

I

I

T

V

R

D

E

G

N

G

G

G

-

R

K

I

V

E

T

K

G

I

I

V

V

E
|
E

Y

H

K

K

D

D

A

A

S

T

P

D

E

E

E

Q

R

R

A

Q

V

I

T

Q

L

E

C

I

N
|
N

S
x
T

G

G

L

I

M

L

A

I

V

A

R

N

S

G

A

A

D

D

L

M

F

K

A

R

L

W

L

L

D

A

R

K

L

L

G

T

N

N

D

N

N

N

A

A

A

Q

S

G

E

E

Y

Y

Y
|
Y

L

I

T
|
T

D

D

I

I

V

I

E

A

L

L

A

A

N

Y

A

Q

H

E

V

G

R

R

G

E

-

I

A

V

A

A

V

V

I

H

E

P

T

Q

D

R

A

L

A

S

E

E

V

V

A

E

G

G

V

V

N
|
N

S

N

R

R

S

L

D

Q

L

L

A

S

A

R

V

L

E

E

A

R

A

V

W

Y

Q

Q

Q

S

V

E

R

Q

R

A

A

E

R

K

A

L

M

L

A

L

E

A

G

G

V

V

T

M

L

L

T

R

A

D

P

P

E

A

T

R

V

F

W

D

F

L

A

R

Y

G

D

T

T

L

V

T

L

H

G

G

R

R

D

D

V

V

T

E

V

I

E

D

P

T

N

N

V

V

V

I

F

I

G

E

P

G

G

N

V

V

T

T

-

L

-

G

-

H

-

R

-

V

-

K

-

I

-

G

-

T

-

G

-

C

-

V

-

I

-

K

-

N

-

S

-

V

V

I

A

G

D

D

V

C

N

E

I

I

R

S

A

P

F

Y

S

T

H

V

I

V

E

E

G

D

A

A

S

N

I

L

A

A

T

A

G

A

A

C

E

T

I

I

G

G

P

P

Y

F

A

A

R

R

L

L

R

R

P

P

G

G

T

A

V

E

V

L

G

L

E

E

K

G

A

A

K

H

I

V

G

G

N

N

F

F

V

V

E

E

T

M

K

K

T

A

V

R

L

L

G

G

K

K

G

G

A

S

K
|
K

A

A

N

G

H
|
H

L

L

T

T

Y

Y

L

L

G

G

D

D

A

A

E

E

V

I

G

G

A

D

G

N

A

V

N
|
N

I

I

G

G

A

A

G

G

T

T

I

I

T

T

C

C

N

N

Y

Y

D

D

G

G

F

A

F

N

K

K

Y

F

G

K

T

T

R

I

I

I

G

G

A

D

G

D

A

V

F
|
F

I

V

G
|
G

S
|
S

N

D

S

T

A

Q

L

L

V

V

A

A

P

P

V

V

A

T

I

V

G

G

E

K

G

G

A

A

I

T

V

I

A
|
A

G

G

A

T

V

T

V

V

T

T

K

R

D

N

V

V

E

G

A

E

D

N

A

A

L

L

A

A

L
x
I

V

S

R
|
R

P

V

E

P

Q

Q

V

T

A

Q

K

K

P

E

G

G

W

W

A

R

K

R

active site: R16 (= R18)
binding coenzyme a: F400 (= F389), G402 (= G391), S403 (= S392), A420 (= A409), A421 (= A410), I436 (≠ L425), R438 (= R427)
binding 2-amino-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: K358 (= K347), H361 (= H350), N375 (= N364)
binding magnesium ion: D103 (= D106), N225 (= N231)
binding uridine-diphosphate-n-acetylglucosamine: L9 (= L11), A10 (= A12), A11 (= A13), G12 (= G14), R16 (= R18), K23 (= K25), Q74 (= Q76), Q77 (= Q79), L78 (= L80), G79 (= G81), T80 (= T82), A83 (= A85), Y101 (= Y104), G102 (= G105), D103 (= D106), Y137 (= Y143), G138 (= G144), E152 (= E159), N167 (= N174), T168 (≠ S175), Y195 (= Y202), T197 (= T204), N225 (= N231)

1hv9B Structure of e. Coli glmu: analysis of pyrophosphorylase and acetyltransferase active sites (see paper)
42% identity, 97% coverage: 3:438/451 of query aligns to 1:449/450 of 1hv9B

query
sites
1hv9B

T

N

S

N

P

A

I

M

A

S

A

V

V

V

I

I

L
|
L

A
|
A

G
|
G

S

K

G

G

T

T

R
|
R

M

M

K

Y

S

S

D

D

L

L

H

P

K
|
K

V

V

L

L

H

H

P

T

I

L

A

A

G

G

R

K

P

A

M

M

L

V

L

Q

H

H

L

V

I

I

D

D

T

A

V

A

A

N

S

E

L

L

S

G

P

A

E

A

R

H

A

V

V

H

V

L

V

V

V

Y

G

G

A

H

R

G

E

D

Q

L

V

L

E

K

A

Q

A

A

V

L

A

K

P

D

H

D

G

N

V

L

A

N

S

W

A

V

H

L

Q
|
Q

A

A

E

E

Q
|
Q

L

L

G
|
G

T
|
T

G

G

H

H

A
|
A

V

M

M

Q

Q

Q

A

A

R

A

E

P

A

F

L

F

A

A

G

D

F

-

D

D

G

E

D

D

V

I

L

L

V

M

L

L

Y
|
Y

G
|
G

D
|
D

V

V

P

P

L

L

V

I

T

S

E

V

E

E

T

T

M

L

R

Q

R

R

M

L

V

-

G

-

R

-

L

-

H

-

E

-

S

R

D

D

A

A

P

K

-

P

-

Q

-

G

S

G

V

I

V

G

V

L

L

L

G

T

F

V

R

K

P

L

A

D

D

D

A

P

A

T

A

G

Y
|
Y

G
|
G

R

R

V

I

I

T

V

R

D

E

G

N

G

G

G

-

R

K

I

V

E

T

K

G

I

I

V

V

E
|
E

Y

H

K

K

D

D

A

A

S

T

P

D

E

E

E

Q

R

R

A

Q

V

I

T

Q

L

E

C

I

N
|
N

S
x
T

G

G

L

I

M

L

A

I

V

A

R

N

S

G

A

A

D

D

L

M

F

K

A

R

L

W

L

L

D

A

R

K

L

L

G

T

N

N

D

N

N

N

A

A

A

Q

S

G

E

E

Y

Y

Y
|
Y

L

I

T
|
T

D

D

I

I

V

I

E

A

L

L

A

A

N

Y

A

Q

H

E

V

G

R

R

G

E

-

I

A

V

A

A

V

V

I

H

E

P

T

Q

D

R

A

L

A

S

E

E

V

V

A

E

G

G

V

V

N
|
N

S

N

R

R

S

L

D

Q

L

L

A

S

A

R

V

L

E

E

A

R

A

V

W

Y

Q

Q

Q

S

V

E

R

Q

R

A

A

E

R

K

A

L

M

L

A

L

E

A

G

G

V

V

T

M

L

L

T

R

A

D

P

P

E

A

T

R

V

F

W

D

F

L

A

R

Y

G

D

T

T

L

V

T

L

H

G

G

R

R

D

D

V

V

T

E

V

I

E

D

P

T

N

N

V

V

V

I

F

I

G

E

P

G

G

N

V

V

T

T

-

L

-

G

-

H

-

R

-

V

-

K

-

I

-

G

-

T

-

G

-

C

-

V

-

I

-

K

-

N

-

S

-

V

V

I

A

G

D

D

V

C

N

E

I

I

R

S

A

P

F

Y

S

T

H

V

I

V

E

E

G

D

A

A

S

N

I

L

A

A

T

A

G

A

A

C

E

T

I

I

G

G

P

P

Y

F

A

A

R

R

L

L

R

R

P

P

G

G

T

A

V

E

V

L

G

L

E

E

K

G

A

A

K

H

I

V

G

G

N

N

F

F

V

V

E

E

T

M

K

K

T

A

V

R

L

L

G

G

K

K

G

G

A

S

K

K

A

A

N

G

H

H

L

L

T

T

Y

Y

L

L

G

G

D

D

A

A

E

E

V

I

G

G

A

D

G

N

A

V

N

N

I

I

G

G

A

A

G

G

T

T

I

I

T

T

C

C

N

N

Y

Y

D

D

G

G

F

A

F

N

K

K

Y

F

G

K

T

T

R

I

I

I

G

G

A

D

G

D

A

V

F

F

I

V

G
|
G

S
|
S

N

D

S

T

A

Q

L

L

V

V

A

A

P

P

V

V

A

T

I

V

G

G

E

K

G

G

A

A

I

T

V

I

A
|
A

G

G

A

T

V

T

V

V

T

T

K

R

D

N

V

V

E

G

A

E

D

N

A

A

L
|
L

A

A

L
x
I

V

S

R
|
R

P

V

E

P

Q

Q

V

T

A

Q

K

K

P

E

G

G

W

W

A

R

K

R

active site: R16 (= R18)
binding cobalt (ii) ion: D103 (= D106), N225 (= N231)
binding coenzyme a: G402 (= G391), S403 (= S392), A420 (= A409), A421 (= A410), L434 (= L423), I436 (≠ L425), R438 (= R427)
binding uridine-diphosphate-n-acetylglucosamine: L9 (= L11), A10 (= A12), A11 (= A13), G12 (= G14), R16 (= R18), K23 (= K25), Q74 (= Q76), Q77 (= Q79), G79 (= G81), T80 (= T82), A83 (= A85), Y101 (= Y104), G102 (= G105), D103 (= D106), Y137 (= Y143), G138 (= G144), E152 (= E159), N167 (= N174), T168 (≠ S175), Y195 (= Y202), T197 (= T204), N225 (= N231)

2oi7A E. Coli glmu- complex with udp-glcnac, desulpho-coa and glcnac-1-po4 (see paper)
42% identity, 96% coverage: 4:438/451 of query aligns to 1:448/449 of 2oi7A

query
sites
2oi7A

S

N

P

A

I

M

A

S

A

V

V

V

I

I

L
|
L

A
|
A

G
|
G

S

K

G

G

T

T

R
|
R

M

M

K

Y

S

S

D

D

L

L

H

P

K

K

V

V

L

L

H

H

P

T

I

L

A

A

G

G

R

K

P

A

M

M

L

V

L

Q

H

H

L

V

I

I

D

D

T

A

V

A

A

N

S

E

L

L

S

G

P

A

E

A

R

H

A

V

V

H

V

L

V

V

V

Y

G

G

A

H

R

G

E

D

Q

L

V

L

E

K

A

Q

A

A

V

L

A

K

P

D

H

D

G

N

V

L

A

N

S

W

A

V

H

L

Q
|
Q

A

A

E

E

Q
|
Q

L
|
L

G
|
G

T
|
T

G

G

H

H

A
|
A

V

M

M

Q

Q

Q

A

A

R

A

E

P

A

F

L

F

A

A

G

D

F

-

D

D

G

E

D

D

V

I

L

L

V

M

L

L

Y
|
Y

G

G

D
|
D

V

V

P

P

L

L

V

I

T

S

E

V

E

E

T

T

M

L

R

Q

R

R

M

L

V

-

G

-

R

-

L

-

H

-

E

-

S

R

D

D

A

A

P

K

-

P

-

Q

-

G

S

G

V

I

V

G

V

L

L

L

G

T

F

V

R

K

P

L

A

D

D

D

A

P

A

T

A

G

Y
|
Y

G
|
G

R

R

V

I

I

T

V

R

D

E

G

N

G

G

G

-

R

K

I

V

E

T

K

G

I

I

V

V

E
|
E

Y

H

K

K

D

D

A

A

S

T

P

D

E

E

E

Q

R

R

A

Q

V

I

T

Q

L

E

C

I

N
|
N

S
x
T

G

G

L

I

M

L

A

I

V

A

R

N

S

G

A

A

D

D

L

M

F

K

A

R

L

W

L

L

D

A

R

K

L

L

G

T

N

N

D

N

N

N

A

A

A

Q

S

G

E

E

Y

Y

Y
|
Y

L

I

T
|
T

D

D

I

I

V

I

E

A

L

L

A

A

N

Y

A

Q

H

E

V

G

R

R

G

E

-

I

A

V

A

A

V

V

I

H

E

P

T

Q

D

R

A

L

A

S

E

E

V

V

A

E

G

G

V

V

N

N

S

N

R

R

S

L

D

Q

L

L

A

S

A

R

V

L

E

E

A

R

A

V

W

Y

Q

Q

Q

S

V

E

R

Q

R

A

A

E

R

K

A

L

M

L

A

L

E

A

G

G

V

V

T

M

L

L

T

R

A

D

P

P

E

A

T

R

V

F

W

D

F

L

A

R

Y

G

D

T

T

L

V

T

L

H

G

G

R

R

D

D

V

V

T

E

V

I

E

D

P

T

N

N

V

V

V

I

F

I

G

E

P

G

G

N

V

V

T

T

-

L

-

G

-

H

-

R

-

V

-

K

-

I

-

G

-

T

-

G

-

C

-

V

-

I

-

K

-

N

-

S

-

V

V

I

A

G

D

D

V

C

N

E

I

I

R

S

A

P

F

Y

S

T

H

V

I

V

E

E

G

D

A

A

S

N

I

L

A

A

T

A

G

A

A

C

E

T

I

I

G

G

P

P

Y

F

A

A

R

R

L

L

R

R

P

P

G

G

T

A

V

E

V

L

G

L

E

E

K

G

A

A

K

H

I

V

G

G

N

N

F

F

V

V

E

E

T

M

K

K

T

A

V

R

L

L

G

G

K

K

G

G

A

S

K
|
K

A

A

N

G

H
|
H

L

L

T

T

Y

Y

L

L

G

G

D

D

A

A

E

E

V

I

G

G

A

D

G

N

A

V

N
|
N

I
|
I

G
|
G

A
|
A

G

G

T

T

I

I

T

T

C

C

N

N

Y

Y

D

D

G

G

F

A

F

N

K

K

Y

F

G

K

T

T

R

I

I

I

G

G

A

D

G

D

A

V

F

F

I

V

G
|
G

S
|
S

N

D

S

T

A

Q

L

L

V

V

A

A

P

P

V

V

A

T

I

V

G

G

E

K

G

G

A

A

I

T

V

I

A
|
A

G

G

A

T

V

T

V

V

T

T

K

R

D

N

V

V

E

G

A

E

D

N

A

A

L

L

A

A

L
x
I

V

S

R
|
R

P

V

E
x
P

Q

Q

V

T

A

Q

K

K

P

E

G

G

W

W

A

R

K

R

active site: R15 (= R18)
binding desulfo-coenzyme a: G401 (= G391), S402 (= S392), A419 (= A409), A420 (= A410), I435 (≠ L425), R437 (= R427), P439 (≠ E429)
binding 2-acetamido-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: K357 (= K347), H360 (= H350), N374 (= N364), I375 (= I365), G376 (= G366), A377 (= A367)
binding uridine-diphosphate-n-acetylglucosamine: L8 (= L11), A9 (= A12), A10 (= A13), G11 (= G14), Q73 (= Q76), Q76 (= Q79), L77 (= L80), G78 (= G81), T79 (= T82), A82 (= A85), Y100 (= Y104), D102 (= D106), Y136 (= Y143), G137 (= G144), E151 (= E159), N166 (= N174), T167 (≠ S175), Y194 (= Y202), T196 (= T204)

2oi5A E. Coli glmu- complex with udp-glcnac and acetyl-coa (see paper)
42% identity, 96% coverage: 4:438/451 of query aligns to 1:448/449 of 2oi5A

query
sites
2oi5A

S

N

P

A

I

M

A

S

A

V

V

V

I

I

L
|
L

A
|
A

G
|
G

S

K

G

G

T

T

R
|
R

M

M

K

Y

S

S

D

D

L

L

H

P

K

K

V

V

L

L

H

H

P

T

I

L

A

A

G

G

R

K

P

A

M

M

L

V

L

Q

H

H

L

V

I

I

D

D

T

A

V

A

A

N

S

E

L

L

S

G

P

A

E

A

R

H

A

V

V

H

V

L

V

V

V

Y

G

G

A

H

R

G

E

D

Q

L

V

L

E

K

A

Q

A

A

V

L

A

K

P

D

H

D

G

N

V

L

A

N

S

W

A

V

H

L

Q
|
Q

A

A

E

E

Q
|
Q

L
|
L

G
|
G

T
|
T

G

G

H

H

A

A

V

M

M

Q

Q

Q

A

A

R

A

E

P

A

F

L

F

A

A

G

D

F

-

D

D

G

E

D

D

V

I

L

L

V

M

L

L

Y
|
Y

G

G

D
|
D

V

V

P

P

L

L

V

I

T

S

E

V

E

E

T

T

M

L

R

Q

R

R

M

L

V

-

G

-

R

-

L

-

H

-

E

-

S

R

D

D

A

A

P

K

-

P

-

Q

-

G

S

G

V

I

V

G

V

L

L

L

G

T

F

V

R

K

P

L

A

D

D

D

A

P

A

T

A

G

Y
|
Y

G
|
G

R

R

V

I

I

T

V

R

D

E

G

N

G

G

G

-

R

K

I

V

E

T

K

G

I

I

V

V

E
|
E

Y

H

K

K

D

D

A

A

S

T

P

D

E

E

E

Q

R

R

A

Q

V

I

T

Q

L

E

C

I

N
|
N

S
x
T

G

G

L

I

M

L

A

I

V

A

R

N

S

G

A

A

D

D

L

M

F

K

A

R

L

W

L

L

D

A

R

K

L

L

G

T

N

N

D

N

N

N

A

A

A

Q

S

G

E

E

Y

Y

Y
|
Y

L

I

T
|
T

D

D

I

I

V

I

E

A

L

L

A

A

N

Y

A

Q

H

E

V

G

R

R

G

E

-

I

A

V

A

A

V

V

I

H

E

P

T

Q

D

R

A

L

A

S

E

E

V

V

A

E

G

G

V

V

N

N

S

N

R

R

S

L

D

Q

L

L

A

S

A

R

V

L

E

E

A

R

A

V

W

Y

Q

Q

Q

S

V

E

R

Q

R

A

A

E

R

K

A

L

M

L

A

L

E

A

G

G

V

V

T

M

L

L

T

R

A

D

P

P

E

A

T

R

V

F

W

D

F

L

A

R

Y

G

D

T

T

L

V

T

L

H

G

G

R

R

D

D

V

V

T

E

V

I

E

D

P

T

N

N

V

V

V

I

F

I

G

E

P

G

G

N

V

V

T

T

-

L

-

G

-

H

-

R

-

V

-

K

-

I

-

G

-

T

-

G

-

C

-

V

-

I

-

K

-

N

-

S

-

V

V

I

A

G

D

D

V

C

N

E

I

I

R

S

A

P

F

Y

S

T

H

V

I

V

E

E

G

D

A

A

S

N

I

L

A

A

T

A

G

A

A

C

E

T

I

I

G

G

P

P

Y

F

A

A

R

R

L

L

R

R

P

P

G

G

T

A

V

E

V

L

G

L

E

E

K

G

A

A

K

H

I

V

G

G

N

N

F

F

V

V

E

E

T

M

K

K

T

A

V

R

L

L

G

G

K

K

G

G

A

S

K

K

A

A

N

G

H
|
H

L

L

T

T

Y

Y

L

L

G

G

D

D

A

A

E

E

V

I

G

G

A

D

G

N

A

V

N

N

I

I

G
|
G

A
|
A

G

G

T

T

I

I

T

T

C

C

N

N

Y

Y

D

D

G

G

F

A

F

N

K

K

Y

F

G

K

T

T

R

I

I

I

G

G

A

D

G

D

A

V

F
|
F

I

V

G
|
G

S
|
S

N

D

S

T

A

Q

L

L

V

V

A

A

P

P

V

V

A

T

I

V

G

G

E

K

G

G

A

A

I

T

V

I

A
|
A

G

G

A

T

V

T

V

V

T

T

K

R

D

N

V

V

E

G

A

E

D

N

A

A

L

L

A

A

L
x
I

V

S

R
|
R

P

V

E

P

Q

Q

V

T

A

Q

K

K

P

E

G

G

W

W

A

R

K

R

active site: R15 (= R18)
binding acetyl coenzyme *a: H360 (= H350), G376 (= G366), A377 (= A367), F399 (= F389), G401 (= G391), S402 (= S392), A419 (= A409), A420 (= A410), I435 (≠ L425), R437 (= R427)
binding uridine-diphosphate-n-acetylglucosamine: L8 (= L11), A9 (= A12), A10 (= A13), G11 (= G14), Q73 (= Q76), Q76 (= Q79), L77 (= L80), G78 (= G81), T79 (= T82), Y100 (= Y104), D102 (= D106), Y136 (= Y143), G137 (= G144), E151 (= E159), N166 (= N174), T167 (≠ S175), Y194 (= Y202), T196 (= T204)

4fceA Crystal structure of yersinia pestis glmu in complex with alpha-d- glucosamine 1-phosphate (gp1)
42% identity, 96% coverage: 4:436/451 of query aligns to 1:441/441 of 4fceA

query
sites
4fceA

S

S

P

S

I

M

A

S

A

V

V

V

I

I

L

L

A

A

G

G

S

K

G

G

T

T

R
|
R

M

M

K

Y

S

S

D

D

L

L

H

P

K

K

V

V

L

L

H

H

P

P

I

L

A

A

G

G

R

K

P

P

M

M

L

V

L

Q

H

H

L

V

I

I

D

D

T

A

V

A

A

M

S

K

L

L

S

G

P

A

E

Q

R

H

A

V

V

H

V

L

V

V

V

Y

G

G

A

H

R

G

E

E

Q

L

V

L

E

K

A

K

A

T

V

L

A

N

P

-

H

-

G

-

V

-

A

-

S

W

A

V

H

L

Q

Q

A

A

E

E

Q

Q

L

L

G

G

T

T

G

G

H

H

A

A

V

M

M

Q

Q

Q

A

A

R

A

E

P

A

H

L

F

A

A

G

D

F

-

D

D

G

E

D

D

V

I

L

L

V

M

L

L

Y

Y

G

G

D

D

V

V

P

P

L

L

V

I

T

S

E

V

E

D

T

T

M

L

R

Q

R

R

M

L

V

L

G

A

R

A

L

K

H

P

E

E

S

G

D

-

A

-

P

-

S

G

V

I

V

G

V

L

L

L

G

T

F

V

R

K

P

L

A

D

D

N

A

P

A

S

A

G

Y

Y

G

G

R

R

V

I

I

V

V

R

D

E

G

N

G

G

G

-

R

D

I

V

E

V

K

G

I

I

V

V

E

E

Y

H

K

K

D

D

A

A

S

S

P

D

E

A

E

Q

R

R

A

E

V

I

T

N

L

E

C

I

N

N

S

T

G

G

L

I

M

L

A

V

V

A

R

N

S

G

A

R

D

D

L

L

F

K

A

R

L

W

L

L

D

S

R

L

L

L

G

D

N

N

D

N

N

N

A

A

A

Q

S

G

E

E

Y

F

Y

Y

L

I

T

T

D

D

I

I

V

I

E

A

L

L

A

A

N

H

A

A

H

D

V

G

R

K

G

K

A

I

A

A

V

T

I

V

E

H

-

P

T

T

D

R

A

L

A

S

E

E

V

V

A

E

G

G

V

V

N

N

S

N

R

R

S

L

D

Q

L

L

A

S

A

A

V

L

E

E

A

R

A

V

W

F

Q

Q

Q

T

V

E

R

Q

R

A

A

E

R

K

A

L

M

L

A

L

E

A

G

G

V

V

T

M

L

L

T

L

A

D

P

P

E

S

T

R

V

F

W

D

F

L

A

R

Y

G

D

E

T

L

V

T

L

H

G

G

R

R

D

D

V

I

T

T

V

I

E

D

P

T

N

N

V

V

V

I

F

I

-

E

-

G

-

H

-

V

-

I

-

L

-

G

-

D

-

R

-

V

-

R

-

I

G

G

P

T

G

G

V

C

T

V

-

L

-

K

-

N

-

C

-

V

V

I

A

G

D

D

V

S

N

E

I

I

R

S

A

P

F

Y

S

T

H

V

I

L

E

E

G

D

A

A

S

R

I

L

A

D

T

A

G

N

A

C

E

T

I

V

G

G

P

P

Y

F

A

A

R

R

L

L

R
|
R

P

P

G

G

T

A

V

E

V

L

G

A

E

E

K

G

A

A

K

H

I

V

G

G

N

N

F

F

V

V

E

E

T

I

K
|
K

K

K

T

A

V

R

L

L

G

G

K

K

G

G

A

S

K

K

A

A

N

G

H

H

L

L

T

S

Y
|
Y

L

L

G

G

D

D

A

A

E

E

V

I

G

G

A

A

G

G

A

V

N

N

I

I

G

G

A

A

G

G

T

T

I

I

T

T

C

C

N
|
N

Y
|
Y

D

D

G

G

F

A

F

N

K
|
K

Y

F

G

K

T

T

R

I

I

I

G

G

A

D

G

D

A

V

F

F

I

V

G

G

S

S

N

D

S

T

A

Q

L

L

V

V

A

A

P

P

V

V

A

T

I

V

G

A

E

N

G

G

A

A

I

T

V

I

A

G

A

A

G

G

A

T

V

T

V

V

T

T

K

R

D

D

V

V

E

A

A

E

D

N

A

E

L

L

A

V

L

I

V

S

R

R

P

V

E

K

Q

Q

V

V

A

H

K

I

P

Q

G

G

W

W

active site: R15 (= R18)
binding 2-amino-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: R325 (= R320), K343 (= K338), Y358 (= Y353), N378 (= N373), Y379 (= Y374), K384 (= K379)

P43889 Bifunctional protein GlmU; EC 2.7.7.23; EC 2.3.1.157 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see 3 papers)
40% identity, 98% coverage: 1:441/451 of query aligns to 1:454/456 of P43889

query
sites
P43889

M

M

T

T

T

K

S

K

P

A

I

L

A

S

A

A

V

V

I

I

L
|
L

A
|
A

G
|
G

S

K

G

G

T

T

R

R

M

M

K

Y

S

S

D

D

L

L

H

P

K
|
K

V

V

L

L

H

H

P

T

I

I

A

A

G

G

R

K

P

P

M

M

L

V

L

K

H

H

L

V

I

I

D

D

T

T

V

A

A

H

S

Q

L

L

S

G

P

S

E

E

R

N

A

I

V

H

V

L

V

I

V

Y

G

G

A

H

R

G

E

D

Q

L

V

M

E

R

A

T

A

H

V

L

A

A

P

N

H

E

G

Q

V

V

A

N

S

W

A

V

H

L

Q
|
Q

A

T

E

E

Q

Q

L

L

G
|
G

T
|
T

G

A

H

H

A

A

V

V

M

Q

Q

Q

A

A

R

A

E

P

A

F

L

F

A

K

G

D

F

N

D

E

G

-

D

N

V

I

L

V

V

V

L

L

Y
|
Y

G
|
G

D
|
D

V

A

P

P

L

L

V

I

T

T

E

K

E

E

T

T

M

L

R

E

R

K

M

L

V

I

G

E

R

A

L

K

H

P

E

E

S

N

D

-

A

-

P

-

S

G

V

I

V

A

V

L

L

L

G

T

F

V

R

N

P

L

A

D

D

N

A

P

A

T

A

G

Y

Y

G
|
G

R

R

V

I

I

I

V

R

D

E

G

N

G

G

G

N

R

V

I

V

E

-

K

A

I

I

V

V

E
|
E

Y

Q

K

K

D

D

A

A

S

N

P

A

E

E

E

Q

R

L

A

N

V

I

T

K

L

E

C

V

N
|
N

S

T

G

G

L

V

M

M

A

V

V

S

R

D

S

G

A

A

D

S

L

F

F

K

A

K

L

W

L

L

D

A

R

R

L

V

G

G

N

N

D

N

N

N

A

A

A

Q

S

G

E

E

Y

Y

L

L

T

T

D

D

I

L

V

I

E

A

L

L

A

A

N

N

A

Q

-

D

H

N

V

C

R

Q

G

V

A

V

A

A

V

V

I

Q

E

A

T

T

D

D

A

V

A

M

E

E

V
|
V

A
x
E

G

G

V

A

N

N

S

N

R

R

S

L

D

Q

L

L

A

A

V

L

E

E

A

R

A

Y

W

F

Q

Q

Q

N

V

K

R

Q

R

A

A

S

R

K

A

L

M

L

A

L

E

E

G

G

V

V

T

M

L

I

T

Y

A

D

P

P

E

A

-

R

-

F

-

D

-

L

-

R

-

G

T

T

V

L

W

E

F

H

A

G

Y

K

D

D

T

V

V

E

L

I

G

D

R

V

D

N

V

V

T

I

V

I

E

E

P

G

N

N

V

V

V

K

F

L

G

G

-

D

-

R

-

V

-

K

-

I

-

G

-

T

-

G

-

C

-

V

-

L

P

K

G

N

V

V

T

V

V

I

A

G

D

N

D

D

V

V

N

E

I

I

R

K

A

P

F

Y

S

S

H

V

I

L

E

E

G

D

A

S

S

I

I

V

A

G

T

E

G

K

A

A

E

A

I

I

G

G

P

P

Y

F

A

S

R

R

L

L

R

R

P

P

G

G

T

A

V

E

V

L

G

A

E

A

K

E

A

T

K

H

I

V

G

G

N

N

F

F

V

V

E

E

T

I

K

K

T

S

V

T

L

V

G

G

K

K

G

G

A

S

K

K

A

V

N

N

H

H

L

L

T

T

Y

Y

L

V

G

G

D

D

A

S

E

E

V

I

G

G

A

S

G

N

A

C

N

N

I

I

G

G

A

A

G

G

T

V

I

I

T

T

C

C

N

N

Y

Y

D

D

G

G

F

A

F

N

K

K

Y

F

G

K

T

T

R

I

I

I

G

G

A

D

G

D

A

V

F

F

I

V

G

G

S

S

N

D

S

T

A

Q

L

L

V

V

A

A

P

P

V

V

A

K

I

V

G

A

E

N

G

G

A

A

I

T

V

I

A

G

A

A

G

G

A

T

V

T

V

I

T

T

K

R

D

D

V

V

E

G

A

E

D

N

A

E

L

L

A

V

L

I

V

T

R

R

P

V

E

A

Q

Q

V

R

A

H

K

I

P

Q

G

G

W

W

A

Q

K

R

R

P

F

I

R

K

LAAG 11:14 (= LAAG 11:14) binding
K25 (= K25) mutation to A: No pyrophosphorylase activity.
Q76 (= Q76) binding ; mutation to A: No pyrophosphorylase activity.
GT 81:82 (= GT 81:82) binding
Y103 (= Y104) mutation to A: Reduces the pyrophosphorylase activity.
YGD 103:105 (= YGD 104:106) binding
D105 (= D106) mutation to A: No pyrophosphorylase activity.
G140 (= G144) binding
E154 (= E159) binding
N169 (= N174) binding
V223 (= V227) mutation to A: Reduces slightly the pyrophosphorylase activity.
E224 (≠ A228) mutation to A: Reduces the pyrophosphorylase activity.

4kqlA Hin glmu bound to wg578 (see paper)
40% identity, 96% coverage: 6:438/451 of query aligns to 3:448/450 of 4kqlA

query
sites
4kqlA

I

L

A

S

A

A

V

V

I

I

L
|
L

A
|
A

G
|
G

S

K

G

G

T

T

R
|
R

M

M

K

Y

S

S

D

D

L

L

H

P

K

K

V

V

L

L

H

H

P

T

I

I

A

A

G

G

R

K

P

P

M

M

L

V

L

K

H

H

L

V

I

I

D

D

T

T

V

A

A

H

S

Q

L

L

S

G

P

S

E

E

R

N

A

I

V

H

V

L

V

I

V

Y

G

G

A

H

R

G

E

D

Q

L

V

M

E

R

A

T

A

H

V

L

A

A

P

N

H

E

G

Q

V

V

A

N

S

W

A

V

H

L

Q
|
Q

A

T

E

E

Q
|
Q

L
|
L

G
|
G

T
|
T

G

A

H

H

A
|
A

V

V

M

Q

Q

Q

A

A

R

A

E

P

A

F

L

F

A

K

G

D

F

N

D

E

G

-

D

N

V

I

L

V

V

V

L

L

Y
|
Y

G

G

D
|
D

V

A

P

P

L

L

V

I

T

T

E

K

E

E

T

T

M

L

R

E

R

K

M

L

V

I

G

E

R

A

L

K

H

P

E

E

S

N

D

-

A

-

P

-

S

G

V

I

V

A

V

L

L

L

G

T

F
x
V

R

N

P

L

A

D

D

N

A

P

A

T

A

G

Y
|
Y

G

G

R

R

V

I

I

I

V

R

D

E

G

N

G

G

G

-

R

N

I

V

E

V

K

A

I

I

V

V

E

E

Y

Q

K

K

D

D

A

A

S

N

P

A

E

E

E

Q

R

L

A

N

V

I

T

K

L

E

C

V

N
|
N

S
x
T

G

G

L

V

M

M

A

V

V

S

R

D

S

G

A

A

D

S

L

F

F

K

A

K

L

W

L

L

D

A

R

R

L

V

G

G

N

N

D

N

N

N

A

A

A

Q

S

G

E

E

Y

Y

L

L

T

T

D

D

I

L

V

I

E

A

L

L

A

A

N

N

A

Q

-

D

H

N

V

C

R

Q

G

V

A

V

A

A

V

V

I

Q

E

A

T

T

D

D

A

V

A

M

E

E

V
|
V

A
x
E