SitesBLAST

CV 59:60 (≠ VV 13:14) binding
EK 80:81 (≠ EA 33:34) binding
87:95 (vs. 40:46, 11% identical) binding
H91 (vs. gap) modified: Tele-8alpha-FAD histidine
H109 (≠ S60) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
V219 (= V172) binding

Sites not aligning to the query:

279 S → P: in dbSNP:rs532964
397:402 binding
530 A → G: in dbSNP:rs1805073
646 S → P: in dbSNP:rs1805074

Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
23% identity, 60% coverage: 2:223/368 of query aligns to 41:260/857 of Q63342

query
sites
Q63342

D

D

Q

R

I

A

E

E

C

T

V

V

V

I

I

I

G

G

A

G

G

G

V
x
C

V
|
V

G

G

L

V

A

S

V

L

A

A

R

Y

A

H

L

L

A

A

A

K

R

A

G

G

-

M

R

R

E

D

V

V

I

V

V

L

L

L

E
|
E

A
x
K

A

S

E

E

A

L

I

T

G

A

V
x
G

G
x
S

T
|
T

-
x
W

-
x
H

S
x
A

R
x
G

N
x
L

S

T

E

T

V

Y

I

F

H

H

A

P

G

G

I

I

Y

N

Y

L

P

K

R

K

G

I

S

H

L

Y

K

D

A

S

T

I

L

-

C

-

V

-

R

-

G

-

R

-

E

-

M

K

L

L

Y

Y

D

E

Y

R

C

L

V

E

E

E

H

E

N

T

-

G

-

Q

-

V

V

V

P

G

H

F

S

H

R

Q

C

P

G

G

K

S

L

I

L

R

V

L

A

A

T

T

S

T

S

P

N

E

Q

R

I

V

P

D

Q

E

L

F

E

K

S

Y

I

Q

M

M

H

T

K

R

G

T

R

N

E

W

N

H

G

A

V

T

L

E

D

Q

L

Y

M

I

R

-

I

I

T

E

G

P

D

E

Q

K

A

I

Q

H

A

E

L

L

E

F

P

P

A

L

L

L

-

N

-

M

-

D

E

K

C

I

V

L

A

A

A

G

V

L

F

Y

S

N

P

P

Q

G

T

D

G

G

I

H

V

I

D

D

S

P

H

Y

Q

S

L

L

M

T

L

M

A

A

L

L

Q

A

G

T

D

G

A

A

E

R

R

K

D

Y

G

G

A

V

V

L

F

L

A

K

F

Y

H

P

S

A

P

P

V
|
V

E

T

A

S

I

L

E

K

A

P

S

R

-

P

N

D

G

G

R

T

F

W

I

D

I

V

K

E

V

T

G

-

D

-

A

P

P

Q

A

G

T

S

I

V

S

R

S

A

A

N

C

R

V

I

I

V

N

N

S

A

A

A

G

G

L

F

H
x
W

A

A

N

R

A

E

L

V

A

G

R

K

K

M

I

I

R

-

G

G

L

L

D

D

A

H

R

P

H

L

V

I

P

P

CV 52:53 (≠ VV 13:14) binding
EK 73:74 (≠ EA 33:34) binding
80:88 (vs. 40:46, 11% identical) binding
H84 (vs. gap) modified: Tele-8alpha-FAD histidine
V212 (= V172) binding
W244 (≠ H206) binding

Sites not aligning to the query:

390:395 binding
573:575 binding
669 binding
676:678 binding
737 binding

4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
23% identity, 60% coverage: 2:223/368 of query aligns to 4:223/824 of 4pabB

query
sites
4pabB

D

D

Q

R

I

A

E

E

C

T

V

V

V

I

I
|
I

G
|
G

A
x
G

G
|
G

V
x
C

V
|
V

G

G

L

V

A

S

V

L

A

A

R

Y

A

H

L

L

A

A

A

K

R

A

G

G

-

M

R

R

E

D

V

V

I

V

V

L

L
|
L

E
|
E

A
x
K

A

S

E

E

A

L

I

T

G
x
A

V
x
G

G
x
S

T
|
T

-

W

-
x
H

S
x
A

R
x
G

N
x
L

S

T

E
x
T

V

Y

I

F

H

H

A

P

G

G

I

I

Y

N

Y

L

P

K

R

K

G

I

S

H

L

Y

K

D

A

S

T

I

L

-

C

-

V

-

R

-

G

-

R

-

E

-

M

K

L

L

Y

Y

D

E

Y

R

C

L

V

E

E

E

H

E

N

T

-

G

-

Q

-

V

V

V

P

G

H

F

S

H

R

Q

C

P

G

G

K

S

L

I

L

R

V

L

A

A

T

T

S

T

S

P

N

E

Q

R

I

V

P

D

Q
x
E

L

F

E

K

S

Y

I

Q

M

M

H

T

K

R

G

T

R

N

E

W

N

H

G

A

V

T

L

E

D

Q

L

Y

M

I

R

-

I

I

T

E

G

P

D

E

Q

K

A

I

Q

H

A

E

L

L

E

F

P

P

A

L

L

L

-

N

-

M

-

D

E

K

C

I

V

L

A

A

A

G

V

L

F

Y

S

N

P

P

Q

G

T

D

G

G

I

H

V

I

D

D

S

P

H

Y

Q

S

L

L

M

T

L

M

A

A

L

L

Q

A

G

T

D

G

A

A

E

R

R

K

D

Y

G

G

A

V

V

L

F

L

A

K

F

Y

H

P

S

A

P
|
P

V
|
V

E

T

A

S

I

L

E

K

A

P

S

R

-

P

N

D

G

G

R

T

F

W

I

D

I

V

K

E

V

T

G

-

D

-

A

P

P

Q

A

G

T

S

I

V

S

R

S

A

A

N

C

R

V

I

I

V

N

N

S
x
A

A
|
A

G
|
G

L
x
F

H
x
W

A

A

N

R

A

E

L

V

A

G

R

K

K

M

I

I

R

-

G

G

L

L

D

D

A

H

R

P

H

L

V

I

P

P

active site: T53 (≠ E48), E102 (≠ Q101)
binding flavin-adenine dinucleotide: I11 (= I9), G12 (= G10), G13 (≠ A11), G14 (= G12), C15 (≠ V13), V16 (= V14), L35 (= L32), E36 (= E33), K37 (≠ A34), A42 (≠ G39), G43 (≠ V40), S44 (≠ G41), T45 (= T42), H47 (vs. gap), A48 (≠ S43), A49 (≠ S44), G50 (≠ R45), L51 (≠ N46), P174 (= P171), V175 (= V172), A203 (≠ S202), A204 (= A203), G205 (= G204), F206 (≠ L205), W207 (≠ H206)

Sites not aligning to the query:

active site: 226, 255, 536
binding flavin-adenine dinucleotide: 226, 228, 324, 326, 327, 328, 353, 354, 355, 356, 357, 358
binding (6s)-5,6,7,8-tetrahydrofolate: 493, 523, 536, 538, 550, 612, 613, 630, 632, 639, 641, 680, 700, 701

P40873 Monomeric sarcosine oxidase; MSOX; EC 1.5.3.1 from Arthrobacter sp. (strain TE1826) (see 3 papers)
23% identity, 57% coverage: 5:215/368 of query aligns to 8:216/389 of P40873

query
sites
P40873

E

D

C

V

V

I

V

V

I

V

G
|
G

A
|
A

G
|
G

V
x
S

V
x
M

G
|
G

L

M

A

A

V

A

A

G

R

Y

A

Y

L

L

A

S

A

K

R

Q

G

G

R

V

E

K

V

T

I

L

V

L

L

V

E
x
D

A

S

A

F

E

H

A

P

I

P

G

H

V

T

-

N

G

G

T

S

S

H

R

G

N

D

S

T

E

R

V

I

I

I

-

R

H

H

A

A

G

-

I

-

Y

-

Y

Y

P

G

R

E

G

G

S

R

L

E

K

Y

A

V

T

P

L

F

C

A

V

L

R

R

G

A

R

Q

E

E

M

L

L

W

Y

Y

D

E

Y

L

C

E

V

K

E

E

-

T

H

H

N

H

V

K

P

I

H

F

S

T

R

K

C

T

G

G

K

-

L

V

L

L

V

V

A

F

T

G

S

P

S

K

N

G

Q

E

I

A

P

P

Q

F

L

V

E

A

S

E

I

T

M

M

H

E

K

A

G

A

R

K

E

E

N

H

G

S

V

-

L

L

D

D

L

V

M

D

R

L

I

L

T

E

G

G

D

S

Q

E

A

I

Q

N

A

K

L

R

E

W

P

P

A

G

L

V

-

T

-

V

-

P

E

E

C

N

V

Y

A

N

A

A

V

I

F

F

S

E

P

K

Q

N

T

S

G

G

I

V

V

L

D

F

S

S

H

E

Q

N

L

C

M

I

L

R

A

A

L

Y

Q

R

G

E

D

L

A

A

E

E

R

A

D

N

G

G

A

A

V

K

F

V

A

L

F

T

H

Y

S

T

P

P

V

V

E

E

A

D

I

F

E

E

A

I

S

A

N

E

G

D

R

F

F

V

I

K

I

I

K

Q

V

T

G

-

D

-

A

A

P

Y

A

G

T

S

I

F

S

T

S

A

A

S

C

K

V

L

I

I

N

V

S

S

A

M

G

G

L

A

H

W

A

N

N

S

A

K

L

L

A

L

R

S

K

K

I

L

R

N

G13 (= G10) mutation to A: Loss of activity.
A14 (= A11) mutation A->D,K: Loss of activity.; mutation A->I,V: Decrease in activity.; mutation A->L,W,Y: Weak activity.
G15 (= G12) mutation to A: Loss of activity.
S16 (≠ V13) mutation to P: Weak activity. Loss of activity; when associated with G-17.
M17 (≠ V14) mutation to G: Weak activity. Loss of activity; when associated with P-16.
G18 (= G15) mutation to A: Weak activity.
D36 (≠ E33) mutation to A: Loss of activity.; mutation to E: Almost no change in activity.; mutation to N: 100-fold decrease in activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
265 mutation C->A,D: Decrease in activity. Stabilizes SoxA against chemicals and metal ions.; mutation C->R,S: Almost no change in activity. Stabilizes SoxA against chemicals and metal ions.
318 C→S: Weak activity.

1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
24% identity, 52% coverage: 19:210/368 of query aligns to 20:207/374 of 1y56B

query
sites
1y56B

A

A

R

H

A

E

L

L

A

A

A

K

R

R

G

G

R

E

E

E

V

V

I

T

V

V

L
x
I

E
|
E

A

-

A
x
K

E

R

A

F

I

I

G

G

V
x
S

G
|
G

T
x
S

S
x
T

S
x
F

R
|
R

N
x
C

S
x
G

E
x
T

V
x
G

I

I

H

R

A

Q

G

Q

I

F

Y

-

P

-

R

N

G

D

S

E

L

A

K

N

A

V

T

R

L

V

C

M

V

K

R

R

G

S

R

V

E

E

M

L

L

W

Y

K

D

K

Y

Y

C

S

V

E

E

E

H

Y

N

G

V

F

P

S

H

F

S

K

R

Q

C

T

G

G

K

Y

L

L

L

F

V

L

A

L

T

Y

S

D

N

E

Q

E

I

V

P

K

Q

T

L

F

E

K

S

R

I

N

M

I

H

E

K

I

G

Q

R

N

E

K

N

F

G

G

V

V

L

P

D

T

L

K

M

L

R

-

I

I

T

T

G

P

D

E

Q

E

A

A

Q

K

A

E

L

I

E

V

P

P

A

L

L

L

-

D

-

I

-

S

E

E

C

V

V

I

A

A

V

S

F

W

S

N

P

P

Q

T

T

D

G

G

I

K

V

A

D

D

S

P

H

F

Q

E

L

A

M

T

L

T

A

A

L

F

Q

A

G

V

D

K

A

A

E

K

R

E

D

Y

G

G

A

A

V

K

F

L

A

L

F

E

H

Y

S

T

P
x
E

V
|
V

E

K

A

G

I

F

E

L

A

I

S

E

N

N

G

N

R

E

F

-

I

-

I

I

K

K

V

G

G

V

G

K

D

T

A

N

P

K

A

G

T

I

I

I

S

K

S

T

A

G

C

I

V

V

I

V

N

N

S
x
A

A
x
T

G
x
N

L

A

H
x
W

A

A

N

N

A

L

L
x
I

active site: F44 (≠ S44), G47 (≠ S47), T48 (≠ E48)
binding flavin-adenine dinucleotide: I33 (≠ L32), E34 (= E33), K35 (≠ A35), S40 (≠ V40), G41 (= G41), S42 (≠ T42), T43 (≠ S43), R45 (= R45), C46 (≠ N46), G47 (≠ S47), T48 (≠ E48), G49 (≠ V49), E170 (≠ P171), V171 (= V172), A199 (≠ S202), T200 (≠ A203), N201 (≠ G204), W203 (≠ H206), I207 (≠ L210)
binding flavin mononucleotide: F44 (≠ S44), R45 (= R45), C46 (≠ N46)

Sites not aligning to the query:

active site: 224, 239, 305, 338
binding flavin-adenine dinucleotide: 10, 11, 13, 14, 15, 250, 305, 306, 307, 332, 334, 335, 336, 337, 338
binding flavin mononucleotide: 228, 260, 301, 303

Query Sequence

>H281DRAFT_02167 FitnessBrowser__Burk376:H281DRAFT_02167
MDQIECVVIGAGVVGLAVARALAARGREVIVLEAAEAIGVGTSSRNSEVIHAGIYYPRGS
LKATLCVRGREMLYDYCVEHNVPHSRCGKLLVATSSNQIPQLESIMHKGRENGVLDLMRI
TGDQAQALEPALECVAAVFSPQTGIVDSHQLMLALQGDAERDGAVFAFHSPVEAIEASNG
RFIIKVGGDAPATISSACVINSAGLHANALARKIRGLDARHVPPLYLARGNYFGISGRAP
FSRLIYPMPNEAGLGVHLTIDLGGQARFGPDVEWVDAINYDVDPRRAESFYAAIRAYWPA
LPDNALQPAYAGIRPKLSGPGEPAADFVIQGPAAHGVRGLVNLFGIESPGLTASLAIAQR
VCELSGRA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory