SitesBLAST
Comparing H281DRAFT_04067 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
8cd1X phikz014 (see paper)
75% identity, 97% coverage: 2:76/77 of query aligns to 1:75/77 of 8cd1X
- binding : S1 (≠ A2), R2 (= R3), K9 (= K10), P11 (= P12), T13 (≠ S14), N15 (= N16), I17 (≠ V18), S18 (= S19), A20 (= A21), H21 (≠ N22), N22 (= N23), K23 (= K24), R25 (≠ K26), R27 (= R28), F28 (= F29), L29 (= L30), P30 (= P31), N31 (= N32), Q33 (= Q34), H35 (≠ R36), R36 (= R37), R47 (= R48), R49 (= R50), A52 (≠ N53), K53 (≠ A54), R56 (= R57), K60 (= K61), R61 (≠ N62)
8rd8WD Small ribosomal subunit protein uS5 (see paper)
76% identity, 97% coverage: 2:76/77 of query aligns to 1:75/76 of 8rd8WD
- binding : S1 (≠ A2), R2 (= R3), K9 (= K10), R10 (≠ A11), M12 (= M13), V13 (≠ S14), G14 (= G15), N15 (= N16), S18 (= S19), H19 (= H20), A20 (= A21), N21 (= N22), N22 (= N23), K23 (= K24), R25 (≠ K26), R26 (= R27), R27 (= R28), F28 (= F29), L29 (= L30), P30 (= P31), N31 (= N32), H33 (≠ Q34), H35 (≠ R36), R36 (= R37), R47 (= R48), R49 (= R50), T52 (≠ N53), K53 (≠ A54), R56 (= R57), K60 (= K61), L61 (≠ N62)
8cgvw Tiamulin bound to the 50s subunit (see paper)
72% identity, 97% coverage: 2:76/77 of query aligns to 1:75/77 of 8cgvw
- binding : H35 (≠ R36), D59 (= D60)
- binding : S1 (≠ A2), R2 (= R3), K9 (= K10), V12 (≠ M13), T13 (≠ S14), N15 (= N16), R17 (≠ V18), S18 (= S19), H19 (= H20), A20 (= A21), L21 (≠ N22), N22 (= N23), K25 (= K26), R26 (= R27), R27 (= R28), F28 (= F29), L29 (= L30), P30 (= P31), N31 (= N32), H33 (≠ Q34), H35 (≠ R36), R36 (= R37), R49 (= R50), A52 (≠ N53), K53 (≠ A54), M55 (≠ L56), R56 (= R57), K60 (= K61), K61 (≠ N62)
8a3lw 50S ribosomal protein L36 (see paper)
72% identity, 97% coverage: 2:76/77 of query aligns to 1:75/77 of 8a3lw
- binding : S1 (≠ A2), R2 (= R3), K9 (= K10), T13 (≠ S14), N15 (= N16), R17 (≠ V18), S18 (= S19), H19 (= H20), A20 (= A21), L21 (≠ N22), L21 (≠ N22), N22 (= N23), K25 (= K26), R26 (= R27), R27 (= R28), F28 (= F29), L29 (= L30), P30 (= P31), N31 (= N32), H33 (≠ Q34), H35 (≠ R36), R36 (= R37), R49 (= R50), A52 (≠ N53), K53 (≠ A54), M55 (≠ L56), R56 (= R57), V57 (≠ L58), K60 (= K61)
Sites not aligning to the query:
7uvxW 50S ribosomal protein L28 (see paper)
67% identity, 99% coverage: 1:76/77 of query aligns to 1:76/78 of 7uvxW
- binding Streptothricin F: H20 (= H20), N22 (= N22)
- binding : M1 (= M1), S2 (≠ A2), K3 (≠ R3), K10 (= K10), N16 (= N16), S19 (= S19), A21 (= A21), N22 (= N22), N23 (= N23), K26 (= K26), R27 (= R27), F29 (= F29), E30 (≠ L30), P31 (= P31), N32 (= N32), H34 (≠ Q34), H36 (≠ R36), R37 (= R37), R50 (= R50), T53 (≠ N53), K54 (≠ A54), R57 (= R57), K61 (= K61)
7uvyW 50S ribosomal protein L2 (see paper)
67% identity, 97% coverage: 2:76/77 of query aligns to 1:75/77 of 7uvyW
- binding Streptothricin D: N16 (= N17), V17 (= V18), S18 (= S19), H19 (= H20), N21 (= N22)
- binding : S1 (≠ A2), K2 (≠ R3), K9 (= K10), V12 (≠ M13), V13 (≠ S14), N15 (= N16), S18 (= S19), A20 (= A21), N21 (= N22), N22 (= N23), K23 (= K24), K25 (= K26), R26 (= R27), R27 (= R28), F28 (= F29), E29 (≠ L30), P30 (= P31), N31 (= N32), H33 (≠ Q34), H35 (≠ R36), R36 (= R37), R49 (= R50), T52 (≠ N53), K53 (≠ A54), M55 (≠ L56), R56 (= R57), K60 (= K61)
7jilX 50S ribosomal protein L15 (see paper)
50% identity, 96% coverage: 2:75/77 of query aligns to 1:74/76 of 7jilX
- binding : S1 (≠ A2), R2 (= R3), K9 (= K10), R10 (≠ A11), M12 (= M13), V13 (≠ S14), N15 (= N16), V17 (= V18), S18 (= S19), A20 (= A21), M21 (≠ N22), N22 (= N23), K23 (= K24), K25 (= K26), R26 (= R27), K27 (≠ R28), F28 (= F29), S29 (≠ L30), V30 (≠ P31), N31 (= N32), K34 (≠ S35), K35 (≠ R36), R36 (= R37), R49 (= R50), R52 (≠ N53), K56 (≠ R57), K60 (= K61)
5mmiY Structure of the large subunit of the chloroplast ribosome (see paper)
51% identity, 84% coverage: 3:67/77 of query aligns to 2:66/77 of 5mmiY
- binding : R2 (= R3), K9 (= K10), N12 (≠ M13), K13 (≠ S14), A14 (≠ G15), N15 (= N16), R16 (≠ N17), S18 (= S19), S20 (≠ A21), N21 (= N22), H22 (≠ N23), K25 (= K26), R26 (= R27), Q28 (≠ F29), F29 (≠ L30), V30 (≠ P31), N31 (= N32), Y34 (≠ S35), K35 (≠ R36), R36 (= R37), K47 (≠ R48), R49 (= R50), T52 (≠ N53), K53 (≠ A54), K56 (≠ R57), K60 (= K61), N61 (= N62)
Sites not aligning to the query:
4v61BY Ribosomal Protein S8 (see paper)
49% identity, 84% coverage: 3:67/77 of query aligns to 1:65/76 of 4v61BY
- binding : R1 (= R3), F5 (≠ V7), T6 (= T8), A13 (≠ G15), N14 (= N16), K15 (≠ N17), V16 (= V18), H18 (= H20), S19 (≠ A21), N20 (= N22), H21 (≠ N23), K24 (= K26), L26 (≠ R28), V29 (≠ P31), N30 (= N32), L31 (= L33), Q32 (= Q34), R35 (= R37), W37 (= W39), E39 (= E41), R43 (= R45), Y44 (≠ W46), K52 (≠ A54)
Sites not aligning to the query:
8fmwAZ 30S ribosomal protein S10 (see paper)
38% identity, 87% coverage: 2:68/77 of query aligns to 1:80/91 of 8fmwAZ
- binding : A1 (= A2), R2 (= R3), K9 (= K10), T11 (≠ P12), M12 (= M13), F13 (≠ S14), N15 (= N16), R19 (vs. gap), K20 (vs. gap), K24 (vs. gap), K26 (vs. gap), A29 (vs. gap), Q31 (≠ S19), H32 (= H20), I33 (≠ A21), G34 (≠ N22), K36 (= K24), T37 (= T25), K38 (= K26), R39 (= R27), F41 (= F29), K42 (≠ L30), V43 (≠ P31), N44 (= N32), I46 (≠ Q34), K49 (≠ R37), R57 (= R45), S58 (≠ W46), A65 (≠ N53), R69 (= R57), K73 (= K61)
Sites not aligning to the query:
7p7qZ 30S ribosomal protein S19 (see paper)
45% identity, 70% coverage: 2:55/77 of query aligns to 1:56/61 of 7p7qZ
- binding : A1 (= A2), K2 (≠ R3), R9 (≠ K10), T11 (≠ P12), S12 (≠ M13), S13 (= S14), N15 (= N16), R17 (≠ V18), S18 (= S19), H19 (= H20), A20 (= A21), N22 (= N23), K25 (= K26), R26 (= R27), V28 (≠ F29), K29 (≠ L30), P30 (= P31), N31 (= N32), Q33 (= Q34), R36 (= R37), K42 (≠ E43), K45 (vs. gap), T50 (≠ L49), R51 (= R50), K54 (≠ N53), S55 (≠ A54)
Sites not aligning to the query:
6yweS structure of the mitoribosome from Neurospora crassa in the P/E tRNA bound state (see paper)
44% identity, 77% coverage: 15:73/77 of query aligns to 63:123/179 of 6yweS
- binding : N64 (= N16), S67 (= S19), R69 (≠ A21), N70 (= N22), E71 (≠ N23), I72 (vs. gap), K73 (= K24), R75 (≠ K26), R76 (= R27), W78 (≠ F29), R79 (≠ L30), P80 (= P31), N81 (= N32), Q83 (= Q34), Q84 (≠ S35), K85 (≠ R36), R86 (= R37), T102 (≠ N53), R103 (≠ A54), R106 (= R57), T107 (≠ L58), K110 (= K61), H121 (≠ L71), K122 (≠ R72), A123 (= A73)
Sites not aligning to the query:
- binding magnesium ion: 47, 49
- binding : 2, 5, 17, 19, 45, 47, 48, 49, 61, 62, 124, 125, 157
5mrcS of the yeast mitochondrial ribosome - Class A (see paper)
33% identity, 77% coverage: 15:73/77 of query aligns to 61:120/185 of 5mrcS
- binding : N62 (= N16), N63 (= N17), I64 (≠ V18), S65 (= S19), S67 (≠ A21), K68 (≠ N22), K70 (= K24), R72 (≠ K26), K73 (≠ R27), W75 (≠ F29), L76 (= L30), P77 (= P31), N78 (= N32), K82 (≠ R36), I93 (≠ V47), S94 (≠ R48), I95 (≠ L49), A99 (≠ N53), K100 (≠ A54), L102 (= L56), K103 (≠ R57), T104 (≠ L58), K107 (= K61), K117 (≠ D70), K119 (≠ R72), S120 (≠ A73)
Sites not aligning to the query:
- binding : 1, 2, 3, 4, 8, 10, 11, 12, 13, 25, 26, 34, 43, 44, 45, 46, 47, 50, 56, 59, 60, 121, 122, 124, 133, 136, 140, 148, 149, 151, 152, 153, 172, 175, 177
7f0dX Cryo-em structure of mycobacterium tuberculosis 50s ribosome subunit bound with clarithromycin (see paper)
49% identity, 45% coverage: 1:35/77 of query aligns to 1:35/63 of 7f0dX
- binding : M1 (= M1), A2 (= A2), K10 (= K10), P12 (= P12), F14 (≠ S14), K16 (≠ N16), V18 (= V18), S19 (= S19), H20 (= H20), S21 (≠ A21), H22 (≠ N22), R23 (≠ N23), R24 (≠ K24), T25 (= T25), R27 (= R27), W29 (≠ F29), D30 (≠ L30), P31 (= P31), N32 (= N32), Q34 (= Q34), T35 (≠ S35)
Sites not aligning to the query:
Query Sequence
>H281DRAFT_04067
MARVCQVTGKAPMSGNNVSHANNKTKRRFLPNLQSRRIWVESENRWVRLRISNAGLRLID
KNGIDAVLADLRARGEA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory