SitesBLAST

V174 (= V223) binding
H225 (≠ K298) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
Y259 (vs. gap) Important for catalytic activity; binding ; mutation to F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
VWVT 360:363 (≠ GWAM 408:411) binding

Sites not aligning to the query:

14:15 binding
35:36 binding
45:48 binding
52 binding
539 binding
552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.

3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
27% identity, 64% coverage: 160:440/442 of query aligns to 110:390/827 of 3gsiA

query
sites
3gsiA

G

G

I

I

A

E

Y

G

R

R

E

L

V

L

N

S

P

P

A

A

Q

E

C

C

V

Q

D

E

I

L

E

Y

P

P

G

L

L

L

R

D

W

G

A

E

R

N

A

I

T

-

P

-

V

L

S

G

G

G

I

L

H

H

L

V

P

P

D

S

D

D

E

G

A

L

G

A

D

S

C

A

A

A

R

R

F

A

T

V

R

Q

E

L

L

L

R

I

A

K

I

R

C

T

E

E

R

S

H

A

G

G

V

V

Q

T

F

Y

R

R

F

G

D

S

T

T

R
x
T

V
|
V

T

T

S

G

I

I

D

E

V

Q

R

S

G

G

A

R

V

V

R

T

G

G

V

V

R

Q

V

T

E

-

S

-

E

-

A

-

G

-

R

-

P

-

D

-

G

-

A

A

N

D

G

G

A

-

N

-

G

-

A

-

N

-

G

-

A

-

T

-

G

-

A

-

T

-

G

-

A

-

E

-

I

V

V

I

Q

P

A

A

D

D

S

I

V

V

V

S

A

C

L
x
A

G
|
G

V

F

D
x
W

S

G

A

A

A

K

L

I

L

G

A

A

P

M

L

I

G

G

V

M

K

A

V

V

P

P

L

L

Y

L

P

P

V

L

-

A

K
x
H

G

Q

Y

Y

S

V

A

K

T

T

L

T

P

P

V

V

-

P

-

A

-

Q

-

G

-

R

I

N

D

D

D

Q

E

P

K

N

A

G

P

A

R

R

A

L

A

P

L

I

M

L

-

R

-

H

-

Q

D

D

E

Q

S
x
D

L

L

-
x
Y

-

Y

-

R

-

E

-

H

-

G

-

D

K

R

T

Y

A

G

I

I

T

G

R

S

F

Y

G

A

N

H

N

R

L

P

R

M

V

P

A

V

G

D

T

V

A

D

E

T

L

L

G

G

N

A

R

Y

-

A

-

P

Q

E

T

T

T

V

L

S

R

E

E

H

Q

H

A

M

L

P

Q

S

T

R

L

L

M

D

K

F

V

T

L

L

D

E

D

D

W

F

F

L

P

P

H

A

A

W

A

E

A

A

-

T

-

K

-

Q

-

L

P

P

T

A

S

L

A

A

H

D

F

S

W

E

V

I

-

E

-

D

-

G

-

F

-

N

G
|
G

R
x
I

R
x
F

P

S

M

F

T

T

P

P

D

D

G

G

A

G

P

P

L

L

G

G

P

E

S

S

G

K

-

E

I

L

D

D

N

G

L

F

W

Y

L

V

N

-

L

-

G

-

H

-

G

A

S

E

T

A

G
x
V

W
|
W

A
x
V

M
x
T

-

H

S

S

M

A

G

G

S

V

A

A

R

K

V

A

V

M

A

A

D

E

L

L

I

L

T

T

Q

T

R

G

K

R

P

S

E

E

I

T

D

D

L

L

N

G

G

E

L

C

T

D

L

I

A

T

R

R

Y

F

active site: H222 (≠ K298), Y256 (vs. gap)
binding flavin-adenine dinucleotide: T170 (≠ R222), V171 (= V223), A200 (≠ L277), G201 (= G278), W203 (≠ D280), H222 (≠ K298), Y256 (vs. gap), G330 (= G378), I331 (≠ R379), F332 (≠ R380), V357 (≠ G408), W358 (= W409), V359 (≠ A410), T360 (≠ M411)
binding magnesium ion: D254 (≠ S321)

Sites not aligning to the query:

active site: 549
binding flavin-adenine dinucleotide: 10, 11, 12, 32, 33, 41, 42, 43, 45, 47, 49
binding magnesium ion: 409
binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696

1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
27% identity, 64% coverage: 160:440/442 of query aligns to 111:391/828 of 1pj6A

query
sites
1pj6A

G

G

I

I

A

E

Y

G

R

R

E

L

V

L

N

S

P

P

A

A

Q

E

C

C

V

Q

D

E

I

L

E

Y

P

P

G

L

L

L

R

D

W

G

A

E

R

N

A

I

T

-

P

-

V

L

S

G

G

G

I

L

H

H

L

V

P

P

D

S

D

D

E

G

A

L

G

A

D

S

C

A

A

A

R

R

F

A

T

V

R

Q

E

L

L

L

R

I

A

K

I

R

C

T

E

E

R

S

H

A

G

G

V

V

Q

T

F

Y

R

R

F

G

D

S

T

T

R

T

V
|
V

T

T

S

G

I

I

D

E

V

Q

R

S

G

G

A

R

V

V

R

T

G

G

V

V

R

Q

V

T

E

-

S

-

E

-

A

-

G

-

R

-

P

-

D

-

G

-

A

A

N

D

G

G

A

-

N

-

G

-

A

-

N

-

G

-

A

-

T

-

G

-

A

-

T

-

G

-

A

-

E

-

I

V

V

I

Q

P

A

A

D

D

S

I

V

V

V

S

A

C

L
x
A

G
|
G

V

F

D
x
W

S

G

A

A

A

K

L

I

L

G

A

A

P

M

L

I

G

G

V

M

K

A

V

V

P

P

L

L

Y

L

P

P

V

L

-

A

K
x
H

G

Q

Y

Y

S

V

A

K

T

T

L

T

P

P

V

V

-

P

-

A

-

Q

-

G

-

R

I

N

D

D

D

Q

E

P

K

N

A

G

P

A

R

R

A

L

A

P

L

I

M

L

-

R

-

H

-

Q

D

D

E

Q

S

D

L

L

-
x
Y

-

Y

-

R

-

E

-

H

-

G

-

D

K

R

T

Y

A

G

I

I

T

G

R

S

F

Y

G

A

N

H

N

R

L

P

R

M

V

P

A

V

G

D

T

V

A

D

E

T

L

L

G

G

N

A

R

Y

-

A

-

P

Q

E

T

T

T

V

L

S

R

E

E

H

Q

H

A

M

L

P

Q

S

T

R

L

L

M

D

K

F

V

T

L

L

D

E

D

D

W

F

F

L

P

P

H

A

A

W

A

E

A

A

-

T

-

K

-

Q

-

L

P

P

T

A

S

L

A

A

H

D

F

S

W

E

V

I

-

E

-

D

-

G

-

F

-

N

G
|
G

R
x
I

R

F

P

S

M

F

T

T

P

P

D

D

G

G

A

G

P

P

L

L

G

G

P

E

S

S

G

K

-

E

I

L

D

D

N

G

L

F

W

Y

L

V

N

-

L

-

G

-

H

-

G

A

S

E

T

A

G
x
V

W
|
W

A
x
V

M
x
T

-

H

S

S

M

A

G

G

S

V

A

A

R

K

V

A

V

M

A

A

D

E

L

L

I

L

T

T

Q

T

R

G

K

R

P

S

E

E

I

T

D

D

L

L

N

G

G

E

L

C

T

D

L

I

A

T

R

R

Y

F

active site: H223 (≠ K298), Y257 (vs. gap)
binding flavin-adenine dinucleotide: V172 (= V223), A201 (≠ L277), G202 (= G278), W204 (≠ D280), H223 (≠ K298), Y257 (vs. gap), G331 (= G378), I332 (≠ R379), V358 (≠ G408), W359 (= W409), V360 (≠ A410), T361 (≠ M411)

Sites not aligning to the query:

active site: 550
binding flavin-adenine dinucleotide: 9, 11, 12, 13, 33, 34, 42, 43, 44, 46, 48, 50

1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
27% identity, 64% coverage: 160:440/442 of query aligns to 110:390/827 of 1pj7A

query
sites
1pj7A

G

G

I

I

A

E

Y

G

R

R

E

L

V

L

N

S

P

P

A

A

Q

E

C

C

V

Q

D

E

I

L

E

Y

P

P

G

L

L

L

R

D

W

G

A

E

R

N

A

I

T

-

P

-

V

L

S

G

G

G

I

L

H

H

L

V

P

P

D

S

D

D

E

G

A

L

G

A

D

S

C

A

A

A

R

R

F

A

T

V

R

Q

E

L

L

L

R

I

A

K

I

R

C

T

E

E

R

S

H

A

G

G

V

V

Q

T

F

Y

R

R

F

G

D

S

T

T

R
x
T

V
|
V

T

T

S

G

I

I

D

E

V

Q

R

S

G

G

A

R

V

V

R

T

G

G

V

V

R

Q

V

T

E

-

S

-

E

-

A

-

G

-

R

-

P

-

D

-

G

-

A

A

N

D

G

G

A

-

N

-

G

-

A

-

N

-

G

-

A

-

T

-

G

-

A

-

T

-

G

-

A

-

E

-

I

V

V

I

Q

P

A

A

D

D

S

I

V

V

V

S

A

C

L
x
A

G
|
G

V

F

D
x
W

S

G

A

A

A

K

L

I

L

G

A

A

P

M

L

I

G

G

V

M

K

A

V

V

P

P

L

L

Y

L

P

P

V

L

-

A

K
x
H

G

Q

Y

Y

S

V

A

K

T

T

L

T

P

P

V

V

-

P

-

A

-

Q

-

G

-

R

I

N

D

D

D

Q

E

P

K

N

A

G

P

A

R

R

A

L

A

P

L

I

M

L

-

R

-

H

-

Q

D

D

E

Q

S

D

L

L

-
x
Y

-

Y

-

R

-

E

-

H

-

G

-

D

K

R

T

Y

A

G

I

I

T

G

R

S

F

Y

G

A

N

H

N

R

L

P

R

M

V

P

A

V

G

D

T

V

A

D

E

T

L

L

G

G

N

A

R

Y

-

A

-

P

Q

E

T

T

T

V

L

S

R

E

E

H

Q

H

A

M

L

P

Q

S

T

R

L

L

M

D

K

F

V

T

L

L

D

E

D

D

W

F

F

L

P

P

H

A

A

W

A

E

A

A

-

T

-

K

-

Q

-

L

P

P

T

A

S

L

A

A

H

D

F

S

W

E

V

I

-

E

-

D

-

G

-

F

-

N

G

G

R
x
I

R

F

P

S

M

F

T

T

P

P

D

D

G

G

A

G

P

P

L

L

G

G

P

E

S

S

G

K

-

E

I

L

D

D

N

G

L

F

W

Y

L

V

N

-

L

-

G

-

H

-

G

A

S

E

T

A

G
x
V

W
|
W

A
x
V

M
x
T

-

H

S

S

M

A

G

G

S

V

A

A

R

K

V

A

V

M

A

A

D

E

L

L

I

L

T

T

Q

T

R

G

K

R

P

S

E

E

I

T

D

D

L

L

N

G

G

E

L

C

T

D

L

I

A

T

R

R

Y

F

active site: H222 (≠ K298), Y256 (vs. gap)
binding flavin-adenine dinucleotide: T170 (≠ R222), V171 (= V223), A200 (≠ L277), G201 (= G278), W203 (≠ D280), H222 (≠ K298), Y256 (vs. gap), I331 (≠ R379), V357 (≠ G408), W358 (= W409), V359 (≠ A410), T360 (≠ M411)

Sites not aligning to the query:

active site: 549
binding flavin-adenine dinucleotide: 8, 10, 11, 12, 32, 33, 41, 42, 43, 45, 47, 49
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
28% identity, 37% coverage: 268:431/442 of query aligns to 193:354/364 of 3if9A

query
sites
3if9A

V

V

Q

W

A

A

D

N

S

H

V

V

A

A

L
x
S

G
|
G

V

V

D
x
W

S

S

A

G

A

M

L
x
F

L

F

A

K

P

Q

L

L

G

G

V

L

K

N

V

N

P

A

L

F

Y

L

P

P

V

V

K

K

G

G

Y

-

S

-

A

E

T

C

L

L

P

S

V

V

I

W

D

N

D

D

E

D

K

-

A

I

P

P

R

L

A

T

A

K

L

T

M

L

D

Y

E

H

S

D

L

A

K

C

T
x
Y

A

I

I

V

T

P

R

R

F

K

G

S

N

G

N

R

L

L

R

V

V

V

A

G

G

A

T

T

A

M

E

K

L

P

G

G

N

D

R

W

Q

S

T

E

T

T

L

P

R

D

E

L

Q

G

A

G

L

L

Q

E

T

S

L

V

M

M

K

K

V

K

L

A

D

K

D

T

W

M

F

L

P

P

H

A

A

I

A

Q

A

N

P

M

T

K

S

V

A

D

H

R

F

F

W

W

V

A

G
|
G

R

L

R
|
R

P

P

M

G

T

T

P

K

D

D

G

G

A

K

P

P

L

Y

L

I

G

G

P

R

S

H

G

P

I

E

D

D

N

S

-

R

L

I

W

L

L

F

N

A

L

A

G

G

H
|
H

G
x
F

S
x
R

T
x
N

G
|
G

W
x
I

A

L

M

L

S

A

M

P

G

A

S

T

A

G

R

A

V

L

V

I

A

S

D

D

L

L

I

I

T

M

Q

N

R

K

K

E

P

V

E

N

I

Q

D

D

binding flavin-adenine dinucleotide: S202 (≠ L277), G203 (= G278), W205 (≠ D280), F209 (≠ L284), G300 (= G378), R302 (= R380), H327 (= H404), F328 (≠ G405), R329 (≠ S406), N330 (≠ T407), G331 (= G408), I332 (≠ W409)
binding glycolic acid: Y246 (≠ T324), R302 (= R380), R329 (≠ S406)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49, 173, 174

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
28% identity, 37% coverage: 268:431/442 of query aligns to 193:354/369 of O31616

query
sites
O31616

V

V

Q

W

A

A

D

N

S

H

V

V

A

A

L

S

G

G

V

V

D

W

S

S

A

G

A

M

L

F

A

K

P

Q

L

L

G

G

V

L

K

N

V

N

P

A

L

F

Y

L

P

P

V

V

K

K

G

G

Y

-

S

-

A

E

T

C

L

L

P

S

V

V

I

W

D

N

D

D

E

D

K

-

A

I

P

P

R

L

A

T

A

K

L

T

M

L

D

Y

E

H

S

D

L
x
H

K

C

T

Y

A

I

I

V

T

P

R

R

F

K

G

S

N

G

N

R

L

L

R

V

V

V

A

G

G

A

T

T

A

M

E

K

L

P

G

G

N

D

R

W

Q

S

T

E

T

T

L

P

R

D

E

L

Q

G

A

G

L

L

Q

E

T

S

L

V

M

M

K

K

V

K

L

A

D

K

D

T

W

M

F

L

P

P

H

A

A

I

A

Q

A

N

P

M

T

K

S

V

A

D

H

R

F

F

W

W

V

A

G

G

R

L

R
|
R

P

P

M

G

T

T

P

K

D

D

G

G

A

K

P

P

L

Y

L

I

G

G

P

R

S

H

G

P

I

E

D

D

N

S

-

R

L

I

W

L

L

F

N

A

L

A

G

G

H
|
H

G
x
F

S
x
R

T
x
N

G
|
G

W
x
I

A
x
L

M

L

S

A

M

P

G

A

S

T

A

G

R

A

V

L

V

I

A

S

D

D

L

L

I

I

T

M

Q

N

R

K

K

E

P

V

E

N

I

Q

D

D

H244 (≠ L322) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (= R380) binding
327:333 (vs. 404:410, 29% identical) binding
R329 (≠ S406) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding
51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
174 binding

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
28% identity, 37% coverage: 268:431/442 of query aligns to 193:354/364 of 1ng3A

query
sites
1ng3A

V

V

Q

W

A

A

D

N

S

H

V

V

A

A

L
x
S

G
|
G

V

V

D
x
W

S

S

A

G

A

M

L
x
F

L

F

A

K

P

Q

L

L

G

G

V

L

K

N

V

N

P

A

L

F

Y

L

P

P

V

V

K

K

G

G

Y

-

S

-

A

E

T

C

L

L

P

S

V

V

I

W

D

N

D

D

E

D

K

-

A

I

P

P

R

L

A

T

A

K

L

T

M

L

D

Y

E

H

S

D

L

H

K

C

T
x
Y

A

I

I

V

T

P

R

R

F

K

G

S

N

G

N
x
R

L

L

R

V

V

V

A

G

G

A

T

T

A

M

E

K

L

P

G

G

N

D

R

W

Q

S

T

E

T

T

L

P

R

D

E

L

Q

G

A

G

L

L

Q

E

T

S

L

V

M

M

K

K

V

K

L

A

D

K

D

T

W

M

F

L

P

P

H

P

A

I

A

Q

A

N

P

M

T

K

S

V

A

D

H

R

F

F

W

W

V

A

G
|
G

R

L

R
|
R

P

P

M

G

T

T

P

K

D

D

G

G

A

K

P

P

L

Y

L

I

G

G

P

R

S

H

G

P

I

E

D

D

N

S

-

R

L

I

W

L

L

F

N

A

L

A

G

G

H
|
H

G

F

S
x
R

T
x
N

G
|
G

W
x
I

A

L

M

L

S

A

M

P

G

A

S

T

A

G

R

A

V

L

V

I

A

S

D

D

L

L

I

I

T

M

Q

N

R

K

K

E

P

V

E

N

I

Q

D

D

binding acetylamino-acetic acid: Y246 (≠ T324), R302 (= R380), R329 (≠ S406)
binding flavin-adenine dinucleotide: S202 (≠ L277), G203 (= G278), W205 (≠ D280), F209 (≠ L284), G300 (= G378), R302 (= R380), H327 (= H404), R329 (≠ S406), N330 (≠ T407), G331 (= G408), I332 (≠ W409)
binding phosphate ion: R254 (≠ N332)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49, 174
binding phosphate ion: 89

6j39A Crystal structure of cmis2 with inhibitor (see paper)
28% identity, 74% coverage: 104:430/442 of query aligns to 54:359/368 of 6j39A

query
sites
6j39A

N

S

K

Q

Q

E

R

D

M

L

Q

L

R

R

I

L

A

C

Y

L

Y

A

S

S

R

R

D

E

C

R

L

Y

H

P

E

S

F

F

R

V

S

K

R

E

H

L

P

E

F

A

D

V

Y

S

G

G

R

T

S

S

Q

A

G

G

Y

Y

L

R

Q

R

-

D

-

G

L

V

F

L

R

D

S

A

E

A

Y

F

D

D

V

D

E

E

L

-

A

S

Q

L

P

A

A

A

L

L

A

D

V

G

L

L

R

R

D

N

-

F

-

L

-

A

-

P

A

L

G

G

I

V

A

A

Y

V

R

A

E

P

V

L

N

N

P

A

A

R

Q

R

C

C

V

R

D

E

I

H

E

E

P

P

G

M

L

L

R

-

W

-

A

-

R

-

A

A

T

E

P

S

V

V

S

R

G

G

I

G

H

L

L

L

-

G

P

P

D

D

E

G

A

A

G

V

D

N

C

P

A

R

R

E

F

L

T

T

R

A

E

A

L

L

R

L

A

A

I

-

C

-

E

-

R

-

H

-

G

-

V

-

Q

-

F

-

R

-

F

-

D

-

T

-

R

-

V

-

T

-

S

A

I

I

D

D

V

V

R

R

G

G

A

T

-

L

V

I

R

R

G

R

V

R

R
x
A

V

T

E

E

S

F

E

L

A

A

G

D

G

E

R

R

P

T

D

P

G

G

A

V

N

L

G

L

A

E

N

N

G

G

A

C

N

A

G

-

A

-

T

-

G

-

A

-

T

-

G

-

A

-

E

-

I

-

V

V

Q

H

A

G

D

D

S

R

V

V

V

L

A

S

L
x
A

G

G

V

C

D
x
W

S

T

A

H

A

R

L

L

L

A

A

G

-

L

P

P

L

A

G

G

V

A

K

V

V

P

P

E

L

I

Y

A

P

P

V

A

K

K

G

G

Y

Q

S
x
I

A

L

T

R

L

L

-

R

-

S

-

A

P

P

V

F

I

L

D

-

E

R

K

R

A

A

P

T

R
|
R

A

A

A

V

L

T

M

R

D

G

E

S

S

G

L

V

K
x
Y

T

L

A

-

I

V

T

P

R

R

F

T

G

D

N

G

N

E

L

L

R

V

V

V

A

G

G

A

T

T

A
x
Y

E

E

L

E

G

R

N

D

R

Y

Q

D

T

T

L

V

R

T

E

A

Q

G

A

G

L

V

Q

A

T

E

L

L

M

L

K

G

V

K

L

V

D

L

D

A

W

V

F

L

P

P

H

G

A

A

A

E

P

L

T

E

S

L

A

A

H

E

F

T

W

A

V

A

G

G

R

L

R
|
R

P

P

M

G

T

S

P

P

D

D

G

G

A

L

P

P

L

V

L

L

G

G

P

W

S

T

G

A

I

V

D

P

N

N

L

L

W

L

L

V

N

A

L

T

G

G

H

H

G
x
S

S
x
R

T
x
I

G
|
G

W
x
V

A
x
Q

M

L

S

A

M

P

G

I

S

T

A

A

R

D

V

V

V

M

A

G

D

E

-

M

L

L

I

V

T

T

Q

G

R

R

K

T

P

P

E

E

I

V

binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: R243 (= R314), Y252 (≠ K323), Y267 (≠ A339), R308 (= R380), R334 (≠ S406), I335 (≠ T407)
binding flavin-adenine dinucleotide: A174 (≠ R237), A203 (≠ L277), W206 (≠ D280), I228 (≠ S301), Y252 (≠ K323), R308 (= R380), S333 (≠ G405), R334 (≠ S406), I335 (≠ T407), G336 (= G408), V337 (≠ W409), Q338 (≠ A410)

Sites not aligning to the query:

binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: 44, 46, 49
binding flavin-adenine dinucleotide: 7, 9, 10, 11, 29, 30, 31, 32, 36, 37, 38, 40, 41, 42, 43, 44

6j38A Crystal structure of cmis2 (see paper)
28% identity, 74% coverage: 104:430/442 of query aligns to 54:359/368 of 6j38A

query
sites
6j38A

N

S

K

Q

Q

E

R

D

M

L

Q

L

R

R

I

L

A

C

Y

L

Y

A

S

S

R

R

D

E

C

R

L

Y

H

P

E

S

F

F

R

V

S

K

R

E

H

L

P

E

F

A

D

V

Y

S

G

G

R

T

S

S

Q

A

G

G

Y

Y

L

R

Q

R

-

D

-

G

L

V

F

L

R

D

S

A

E

A

Y

F

D

D

V

D

E

E

L

-

A

S

Q

L

P

A

A

A

L

L

A

D

V

G

L

L

R

R

D

N

-

F

-

L

-

A

-

P

A

L

G

G

I

V

A

A

Y

V

R

A

E

P

V

L

N

N

P

A

A

R

Q

R

C

C

V

R

D

E

I

H

E

E

P

P

G

M

L

L

R

-

W

-

A

-

R

-

A

A

T

E

P

S

V

V

S

R

G

G

I

G

H

L

L

L

-

G

P

P

D

D

E

G

A

A

G

V

D

N

C

P

A

R

R

E

F

L

T

T

R

A

E

A

L

L

R

L

A

A

I

-

C

-

E

-

R

-

H

-

G

-

V

-

Q

-

F

-

R

-

F

-

D

-

T

-

R

-

V

-

T

-

S

A

I

I

D

D

V

V

R

R

G

G

A

T

-

L

V

I

R

R

G

R

V

R

R
x
A

V

T

E

E

S

F

E

L

A

A

G

D

G

E

R

R

P

T

D

P

G

G

A

V

N

L

G

L

A

E

N

N

G

G

A

C

N

A

G

-

A

-

T

-

G

-

A

-

T

-

G

-

A

-

E

-

I

-

V

V

Q

H

A

G

D

D

S

R

V

V

V

L

A

S

L
x
A

G

G

V

C

D
x
W

S

T

A

H

A

R

L

L

L

A

A

G

-

L

P

P

L

A

G

G

V

A

K

V

V

P

P

E

L

I

Y

A

P

P

V

A

K

K

G
|
G

Y

Q

S

I

A

L

T

R

L

L

-

R

-

S

-

A

P

P

V

F

I

L

D

-

E

R

K

R

A

A

P

T

R

R

A

A

A

V

L

T

M

R

D

G

E

S

S

G

L

V

K

Y

T

L

A

-

I

V

T

P

R

R

F

T

G

D

N

G

N

E

L

L

R

V

V

V

A

G

G

A

T

T

A

Y

E

E

L

E

G

R

N

D

R

Y

Q

D

T

T

L

V

R

T

E

A

Q

G

A

G

L

V

Q

A

T

E

L

L

M

L

K

G

V

K

L

V

D

L

D

A

W

V

F

L

P

P

H

G

A

A

A

E

P

L

T

E

S

L

A

A

H

E

F

T

W

A

V

A

G
|
G

R

L

R
|
R

P

P

M

G

T

S

P

P

D

D

G

G

A

L

P

P

L

V

L

L

G

G

P

W

S

T

G

A

I

V

D

P

N

N

L

L

W

L

L

V

N

A

L

T

G

G

H

H

G
x
S

S
x
R

T
x
I

G
|
G

W
x
V

A
x
Q

M

L

S

A

M

P

G

I

S

T

A

A

R

D

V

V

V

M

A

G

D

E

-

M

L

L

I

V

T

T

Q

G

R

R

K

T

P

P

E

E

I

V

binding flavin-adenine dinucleotide: A174 (≠ R237), A203 (≠ L277), W206 (≠ D280), G226 (= G299), G306 (= G378), R308 (= R380), S333 (≠ G405), R334 (≠ S406), I335 (≠ T407), G336 (= G408), V337 (≠ W409), Q338 (≠ A410)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 9, 11, 29, 30, 31, 36, 37, 38, 41, 42, 43, 44

Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
28% identity, 41% coverage: 259:440/442 of query aligns to 195:370/377 of Q5L2C2

query
sites
Q5L2C2

T

T

G

G

A

G

T

T

G

F

A

A

E

E

I

-

V

-

Q

-

A

-

D

-

S

A

V

V

V

I

A

A

L

S

G

G

V

A

D

W

S

A

A

A

A

R

L

L

L

G

A

A

P

R

L

V

G

G

V

L

K

S

V

L

P

S

L

V

Y

Y

P

P

V

V

K

K

G

G

Y

E

S

C

A

V

T

M

L

V

P

-

V

-

I

-

D

-

E

-

K

R

A

A

P

P

R

V

A

P

A

L

L

L

M

Q

D

T

E

T

S

V

L

F

K

A

T

K

-

N

-

G

-

C

-

Y

A

I

I

V

T

P

R

K

F

S

G

G

N

N

N

R

L

L

R

L

V

I

A

G

G

A

T

T

A

S

E

T

L

P

G

G

N

T

R

F

Q

D

T

R

L

V

R

S

E

A

Q

G

A

G

L

V

Q

M

T

N

L

L

M

L

K

H

V

R

L

A

D

A

D

H

W

L

F

V

P

P

H

D

A

I

A

E

A

Q

P

A

T

E

S

W

A

V

H

A

F

S

W

W

V

S

G

G

R

I

R
|
R

P

P

M

Q

T

T

P

E

D

D

G

G

A

L

P

P

L

Y

L

L

G

G

P

E

S

H

-

P

G

E

I

R

D

R

N

G

L

L

W

F

L

V

N

A

L

A

G

G

H
|
H

G
x
Y

S
x
R

T
x
N

G
|
G

W
x
I

A
x
L

M

L

S

S

M

P

G

L

S

T

A

G

R

L

V

L

V

V

A

A

D

D

L

L

I

V

T

E

Q

R

R

K

K

E

P

T

E

A

I

F

D

D

L

L

N

A

G

P

L

F

T

S

L

L

A

T

R

R

Y

H

R309 (= R380) binding
334:340 (vs. 404:410, 29% identical) binding
R336 (≠ S406) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding
180 binding

4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
28% identity, 41% coverage: 259:440/442 of query aligns to 193:368/368 of 4yshB

query
sites
4yshB

T

T

G

G

A

G

T

T

G

F

A

A

E

E

I

-

V

-

Q

-

A

-

D

-

S

A

V

V

V

I

A

A

L
x
S

G

G

V

A

D
x
W

S

A

A

A

A

R

L

L

L

G

A

A

P

R

L

V

G

G

V

L

K

S

V

L

P

S

L

V

Y

Y

P

P

V

V

K

K

G

G

Y

E

S

C

A

V

T

M

L

V

P

-

V

-

I

-

D

-

E

-

K

R

A

A

P

P

R

V

A

P

A

L

L

L

M

Q

D

T

E

T

S

V

L

F

K

A

T

K

-

N

-
x
G

-

C

-
x
Y

A

I

I

V

T

P

R

K

F

S

G

G

N

N

N

R

L

L

R

L

V
x
I

A

G

G
x
A

T

T

A

S

E

T

L

P

G

G

N

T

R

F

Q

D

T

R

L

V

R

S

E

A

Q

G

A

G

L

V

Q

M

T

N

L
|
L

M

L

K

H

V

R

L

A

D

A

D

H

W

L

F

V

P

P

H

D

A

I

A

E

A

Q

P

A

T

E

S

W

A

V

H

A

F

S

W

W

V

S

G
|
G

R

I

R
|
R

P

P

M

Q

T

T

P

E

D

D

G

G

A

L

P

P

L

Y

L

L

G

G

P

E

S

H

-

P

G

E

I

R

D

R

N

G

L

L

W

F

L

V

N

A

L

A

G

G

H
|
H

G

Y

S
x
R

T
x
N

G
|
G

W
x
I

A
x
L

M

L

S

S

M

P

G

L

S

T

A

G

R

L

V

L

V

V

A

A

D

D

L

L

I

V

T

E

Q

R

R

K

K

E

P

T

E

A

I

F

D

D

L

L

N

A

G

P

L

F

T

S

L

L

A

T

R

R

Y

H

active site: I262 (≠ V335), L283 (= L356), G305 (= G378), N335 (≠ T407), L338 (≠ A410)
binding flavin-adenine dinucleotide: S206 (≠ L277), W209 (≠ D280), R307 (= R380), H332 (= H404), R334 (≠ S406), N335 (≠ T407), G336 (= G408), I337 (≠ W409), L338 (≠ A410)
binding glycine: G249 (vs. gap), Y251 (vs. gap), Y251 (vs. gap), A264 (≠ G337), R307 (= R380), R334 (≠ S406), R334 (≠ S406)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48, 178

4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
28% identity, 41% coverage: 259:440/442 of query aligns to 193:368/370 of 4yshA

query
sites
4yshA

T

T

G

G

A

G

T

T

G

F

A

A

E

E

I

-

V

-

Q

-

A

-

D

-

S

A

V

V

V

I

A

A

L
x
S

G
|
G

V

A

D
x
W

S

A

A

A

A

R

L

L

L

G

A

A

P

R

L

V

G

G

V

L

K

S

V

L

P

S

L

V

Y

Y

P

P

V

V

K

K

G

G

Y

E

S

C

A

V

T

M

L

V

P

-

V

-

I

-

D

-

E

-

K

R

A

A

P

P

R

V

A

P

A

L

L

L

M

Q

D

T

E

T

S

V

L

F

K

A

T

K

-

N

-

G

-

C

-

Y

A

I

I

V

T

P

R

K

F

S

G

G

N

N

N

R

L

L

R

L

V
x
I

A

G

G

A

T

T

A

S

E

T

L

P

G

G

N

T

R

F

Q

D

T

R

L

V

R

S

E

A

Q

G

A

G

L

V

Q

M

T

N

L
|
L

M

L

K

H

V

R

L

A

D

A

D

H

W

L

F

V

P

P

H

D

A

I

A

E

A

Q

P

A

T

E

S

W

A

V

H

A

F

S

W

W

V

S

G
|
G

R

I

R
|
R

P

P

M

Q

T

T

P

E

D

D

G

G

A

L

P

P

L

Y

L

L

G

G

P

E

S

H

-

P

G

E

I

R

D

R

N

G

L

L

W

F

L

V

N

A

L

A

G

G

H
|
H

G

Y

S
x
R

T
x
N

G
|
G

W
x
I

A
x
L

M

L

S

S

M

P

G

L

S

T

A

G

R

L

V

L

V

V

A

A

D

D

L

L

I

V

T

E

Q

R

R

K

K

E

P

T

E

A

I

F

D

D

L

L

N

A

G

P

L

F

T

S

L

L

A

T

R

R

Y

H

active site: I262 (≠ V335), L283 (= L356), G305 (= G378), N335 (≠ T407), L338 (≠ A410)
binding flavin-adenine dinucleotide: S206 (≠ L277), G207 (= G278), W209 (≠ D280), R307 (= R380), H332 (= H404), R334 (≠ S406), N335 (≠ T407), G336 (= G408), I337 (≠ W409)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48, 178

S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
27% identity, 38% coverage: 257:423/442 of query aligns to 182:346/369 of S5FMM4

query
sites
S5FMM4

G

G

A

A

T

V

G

R

A

V

T

T

G

S

A

A

A

S

E

G

I

T

V

A

Q

E

A

A

D

E

S

H

V

A

V

V

V

I

A

A

L
x
S

G

G

V

V

D

W

S

S

A

G

A

A

L

L

L

F

A

K

P

Q

L

I

G

G

V

L

K

D

V

K

P

R

L

F

Y

Y

P

P

V

V

K

K

G

G

Y

-

S

-

A

E

T

C

L

L

P

S

V

V

I

W

D

N

D

D

E

G

K

I

A

S

P

L

R

T

A

R

A

T

L

L

M

Y

D

H

E

D

S

H

L

C

K

Y

T

I

A

-

I

V

T

P

R

R

F

H

G

S

N

G

N

R

L

L

R

V

V

V

A

G

G

A

T

T

A

M

E

K

L

P

G

G

N

D

R

W

Q

N

T

E

T

Q

L

P

R

E

E

L

Q

G

A

G

L

I

Q

E

T

E

L

L

M

I

K

R

V

K

L

A

D

K

D

S

W

M

F

L

P

P

H

G

A

I

A

E

A

S

P

M

T

K

S

I

A

D

H

Q

F

C

W

W

V

A

G

G

R

L

R

R

P

P

M

E

T

T

P

G

D

D

G

G

A

N

P

P

L

Y

L

I

G

G

-

R

-

H

P

P

S

E

G

N

I

-

D

D

N

R

L

I

W

L

L

F

N

A

L

A

G

G

H

H

G

F

S

R

T

N

G

G

W
x
I

A

L

M

L

S

A

M

P

G

A

S

T

A

G

R

E

V

M

V
x
M

A

A

D

D

L

M

I

I

S202 (≠ L277) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ W409) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (≠ V419) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

Sites not aligning to the query:

51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
25% identity, 41% coverage: 256:435/442 of query aligns to 179:356/363 of 7cyxA

query
sites
7cyxA

N

N

G

K

A

V

T

T

G

G

A

V

T

I

G

T

A

S

A

E

E

G

I

I

V

V

Q

T

A

C

D

E

S

K

V

V

V

I

A

A

L
x
G

G
|
G

V

S

D
x
W

S

S

A

T

A

K

L

L

A

S

P

Y

L

F

G

H

V

R

K

D

V

W

P

G

L

T

Y

Y

P

P

V

V

K

K

G

G

Y

-

S

-

A

E

T

V

L

V

P

A

V

V

I

R

D

S

D

R

E

K

K

Q

A

L

P

L

R

K

A

A

A

P

L

I

M

F

D

Q

E

E

S

R

L

F

K

Y

T

I

A

T

I

P

T

K

R

R

F

-

G

G

N

G

N

R

L

Y

R

V

V

I

A

G

G

A

T

T

A

M

E

K

L

P

G

H

N

T

R

F

Q

N

T

K

T

T

L

V

R

Q

E

P

Q

E

A

S

L

I

Q

T

T

S

L

I

M

L

K

E

V

R

L

A

D

Y

D

T

W

I

F

L

P

P

H

A

A

L

A

K

A

E

P

A

T

E

S

W

A

E

H

S

F

T

W

W

V

A

G
|
G

R

L

R
|
R

P
|
P

M

Q

T

S

P

N

D

H

G

E

A

A

P

P

L

Y

L

M

G

G

P

E

-

H

S

E

G

E

I

I

D

K

N

G

L

L

W

Y

L

A

N

C

L

T

G

G

H

H

G
x
Y

S
x
R

T
x
N

G
|
G

W
x
I

A

L

M

L

S

S

M

P

G

I

S

S

A

G

R

Q

V

Y

V

M

A

A

D

D

L

L

I

I

T

E

Q

G

R

K

K

Q

P

E

E

N

I

H

D

L

L

L

N

D

G

S

L

L

binding flavin-adenine dinucleotide: G200 (≠ L277), G201 (= G278), W203 (≠ D280), G298 (= G378), R300 (= R380), P301 (= P381), Y326 (≠ G405), R327 (≠ S406), N328 (≠ T407), G329 (= G408), I330 (≠ W409)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 8, 10, 11, 12, 30, 31, 32, 38, 39, 40, 43, 45, 46, 171

4x9mA Oxidized l-alpha-glycerophosphate oxidase from mycoplasma pneumoniae with fad bound (see paper)
47% identity, 11% coverage: 4:50/442 of query aligns to 7:53/384 of 4x9mA

query
sites
4x9mA

I

L

V

I

L

V

G
|
G

G

G

G
|
G

V

V

I
|
I

G

G

V

C

A

A

T

T

A

A

F

Y

Y

E

L

L

R

S

Q

Q

Q

Y

G

K

C

L

E

K

V

V

T

T

V

L

I

V

E
|
E

R
x
K

E

H

P

H

D

Y

V

L

A

A

L

Q

A
x
E

T
|
T

S
|
S

F

H

G
x
A

N
|
N

A
x
S

G

G

V
|
V

I

I

A
x
H

P

T

G

G

binding flavin-adenine dinucleotide: G10 (= G7), G12 (= G9), I14 (= I11), E33 (= E30), K34 (≠ R31), E41 (≠ A38), T42 (= T39), S43 (= S40), A45 (≠ G42), N46 (= N43), S47 (≠ A44), V49 (= V46), H51 (≠ A48)
binding sn-glycerol-3-phosphate: S47 (≠ A44), H51 (≠ A48)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 176, 177, 209, 210, 212, 234, 319, 320, 346, 347, 348, 349, 350, 351, 352
binding sn-glycerol-3-phosphate: 258, 259, 320, 348

P75063 Glycerol 3-phosphate oxidase; GlpO; L-alpha-glycerophosphate oxidase; EC 1.1.3.21 from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) (see paper)
47% identity, 11% coverage: 4:50/442 of query aligns to 7:53/384 of P75063

query
sites
P75063

I

L

V

I

L

V

G

G

V

V

I
|
I

G

G

V

C

A

A

T

T

A

A

F

Y

Y

E

L

L

R

S

Q

Q

Q

Y

G

K

C

L

E

K

V

V

T

T

V

L

I

V

E
|
E

R

K

E

H

P

H

D

Y

V

L

A

A

L

Q

A

E

T
|
T

S
|
S

F

H

G

A

N

N

A
x
S

G
|
G

V
|
V

I

I

A

H

P

T

G

G

I14 (= I11) binding
E33 (= E30) binding
TS 42:43 (= TS 39:40) binding
SGV 47:49 (≠ AGV 44:46) binding

Sites not aligning to the query:

177 binding
346:347 binding
352 binding

Query Sequence

>H281DRAFT_05622 FitnessBrowser__Burk376:H281DRAFT_05622
MKTIVLGGGVIGVATAFYLRQQGCEVTVIEREPDVALATSFGNAGVIAPGYVTPWAAPGM
PTKILKYLFKPASPLIFRPTLDPAQWRWIARWLRECDLERFRVNKQRMQRIAYYSRDCLH
EFRSRHPFDYGRSQGYLQLFRSEYDVELAQPALAVLRDAGIAYREVNPAQCVDIEPGLRW
ARATPVSGIHLPDDEAGDCARFTRELRAICERHGVQFRFDTRVTSIDVRGGAVRGVRVES
EAGGRPDGANGANGANGATGATGAAEIVQADSVVVALGVDSAALLAPLGVKVPLYPVKGY
SATLPVIDDEKAPRAALMDESLKTAITRFGNNLRVAGTAELGNRQTTLREQALQTLMKVL
DDWFPHAAAPTSAHFWVGRRPMTPDGAPLLGPSGIDNLWLNLGHGSTGWAMSMGSARVVA
DLITQRKPEIDLNGLTLARYGK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory