SitesBLAST
Comparing HSERO_RS02375 FitnessBrowser__HerbieS:HSERO_RS02375 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
26% identity, 100% coverage: 1:320/320 of query aligns to 1:307/309 of 4zrnA
- active site: T117 (≠ S118), G119 (vs. gap), A120 (≠ K120), Y143 (= Y144), K147 (= K148), Y181 (vs. gap), G185 (= G178)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D31 (vs. gap), N32 (vs. gap), S34 (vs. gap), S35 (vs. gap), G36 (vs. gap), S51 (≠ T38), I52 (≠ V39), L73 (≠ C72), A74 (= A73), A75 (= A74), T92 (≠ I93), S115 (≠ L116), S116 (= S117), Y143 (= Y144), K147 (= K148), Y170 (≠ P171), V173 (= V174)
- binding uridine-5'-diphosphate-glucose: T117 (≠ S118), G119 (vs. gap), A120 (≠ K120), Y143 (= Y144), N172 (≠ L173), G185 (= G178), V186 (= V179), H201 (≠ P197), F203 (≠ G199), Y208 (≠ N204), R210 (= R206), V244 (≠ T242), R267 (≠ K276), D270 (≠ I279)
6bwlA X-ray structure of pal from bacillus thuringiensis (see paper)
24% identity, 98% coverage: 1:313/320 of query aligns to 1:305/313 of 6bwlA
- active site: T122 (≠ S118), C123 (≠ I119), M124 (≠ K120), Y147 (= Y144), K151 (= K148)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D31 (≠ R29), N32 (≠ G30), L33 (≠ A31), N35 (≠ R33), S36 (≠ P34), D57 (≠ L53), I58 (≠ D54), L79 (≠ C72), A80 (= A73), A81 (= A74), I83 (≠ V76), M120 (≠ L116), K151 (= K148), N176 (≠ L173), T177 (≠ V174)
- binding uridine-5'-diphosphate: N176 (≠ L173), G189 (= G178), V190 (= V179), N205 (≠ P197), I206 (≠ L198), Y207 (≠ G199), Q212 (≠ N204), R214 (= R206), I250 (≠ L253), E275 (≠ G278)
4id9B Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
28% identity, 72% coverage: 3:232/320 of query aligns to 3:251/328 of 4id9B
- active site: S107 (= S118), Y133 (= Y144), K137 (= K148)
- binding nicotinamide-adenine-dinucleotide: G10 (= G10), R11 (≠ F11), V12 (= V12), D31 (≠ A31), L32 (≠ F32), S45 (≠ G52), L46 (= L53), L65 (≠ C72), A67 (= A74), V82 (≠ I93), Y133 (= Y144), K137 (= K148), F160 (vs. gap), S161 (vs. gap), H162 (vs. gap), T163 (vs. gap)
4id9A Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
28% identity, 72% coverage: 3:232/320 of query aligns to 2:250/321 of 4id9A
- active site: S106 (= S118), Y132 (= Y144), K136 (= K148)
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), R10 (≠ F11), V11 (= V12), D30 (≠ A31), L31 (≠ F32), R32 (= R33), S44 (≠ G52), L45 (= L53), L64 (≠ C72), A66 (= A74), V81 (≠ I93), S106 (= S118), Y132 (= Y144), K136 (= K148), F159 (vs. gap), H161 (vs. gap), T162 (vs. gap)
Sites not aligning to the query:
6kv9A Moee5 in complex with udp-glucuronic acid and NAD (see paper)
31% identity, 67% coverage: 3:215/320 of query aligns to 3:208/299 of 6kv9A
- active site: S110 (= S118), S111 (≠ I119), S112 (≠ K120), Y133 (= Y144), K137 (= K148)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D31 (≠ A31), R32 (≠ F32), R33 (= R33), D47 (≠ L53), L48 (≠ D54), L66 (≠ C72), A67 (= A73), A68 (= A74), P70 (≠ V76), C85 (≠ I93), A108 (≠ L116), S109 (= S117), K137 (= K148), F162 (≠ P171), T164 (≠ L173), V165 (= V174), R171 (≠ K180), M174 (vs. gap)
- binding uridine-5'-diphosphate-glucuronic acid: P70 (≠ V76), R73 (≠ M79), S110 (= S118), S111 (≠ I119), Y133 (= Y144), T164 (≠ L173), R171 (≠ K180), M174 (vs. gap), F175 (= F183), R178 (vs. gap), E190 (≠ P197), I191 (≠ L198), Y192 (≠ G199), Q197 (≠ N204), R199 (= R206)
Sites not aligning to the query:
8vr2B Crystal structure of the pcryo_0617 oxidoreductase/decarboxylase from psychrobacter cryohalolentis k5 in the presence of NAD and udp
25% identity, 98% coverage: 3:315/320 of query aligns to 10:309/321 of 8vr2B
- binding nicotinamide-adenine-dinucleotide: G14 (= G7), G17 (= G10), F18 (= F11), I19 (≠ V12), D37 (≠ G30), N38 (≠ A31), E40 (≠ R33), R41 (≠ P34), N61 (vs. gap), V62 (vs. gap), A81 (≠ C72), A82 (= A73), A83 (= A74), F124 (≠ L116), K154 (= K148), P177 (= P171), N179 (≠ L173)
- binding uridine-5'-diphosphate: R147 (vs. gap), G189 (≠ N182), A190 (≠ F183), M194 (= M186), Y205 (≠ P197), I206 (≠ L198), F207 (≠ G199), R214 (= R206), I251 (≠ T243)
Q9LPG6 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM2; NDP-rhamnose synthase; Protein MUCILAGE-MODIFIED 4; Protein RHAMNOSE BIOSYNTHESIS 2; Rhamnose biosynthetic enzyme 2; AtRHM2; UDP-L-rhamnose synthase MUM4; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
27% identity, 72% coverage: 2:232/320 of query aligns to 10:250/667 of Q9LPG6
- G18 (= G10) mutation to A: Abolishes dehydratase activity.
- K36 (≠ E37) mutation to A: Reduces dehydratase activity.
- D96 (≠ M79) mutation to N: In mum4-1; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.
- K165 (= K148) mutation to A: Abolishes dehydratase activity.
- G193 (= G176) mutation to R: In mum4-2; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.
Sites not aligning to the query:
- 392 G→A: No effect on dehydratase activity.
- 413 K→A: No effect on dehydratase activity.
- 518 K→A: No effect on dehydratase activity.
Q9FX01 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1; At3BETAHSD/D1; 4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase isoform 1-1; Reticulon-like protein B24; AtRTNLB24; Sterol-4-alpha-carboxylate 3-dehydrogenase 1, decarboxylating; EC 1.1.1.418 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 98% coverage: 4:317/320 of query aligns to 13:358/439 of Q9FX01
- D41 (vs. gap) Essential for the 3betaHSD/D activity; mutation to V: Lost activity.
- D72 (≠ G52) Essential for the 3betaHSD/D activity; mutation D->A,V: Lost activity.
- T131 (≠ L116) Essential for the 3betaHSD/D activity; mutation to V: Lost activity.
- S133 (= S118) mutation to A: Reduced activity with slower catalysis and lower substrate binding.
- S135 (≠ K120) mutation to A: Normal activity, but slower catalysis with 4alpha-carboxysterol as substrate.; mutation to T: Altered activity due to reduced affinity and catalysis and leading to lower amount of C4-demethylated sterols and higher quantities of 4,4-dimethylated sterol intermediates, and a higher ratio of 4,4-dimethylsterols to 4alpha-methylsterols.; mutation to Y: Altered activity leading to an exclusive production of C4-methylated sterols and 4alpha-carboxy-3beta-hydroxy sterols.
- Y161 (= Y144) Essential for the 3betaHSD/D activity; mutation to F: Lost activity.
- K165 (= K148) Essential for the 3betaHSD/D activity; mutation to I: Lost activity.
- R320 (vs. gap) mutation to I: Normal activity.
- R328 (≠ L287) Essential for the 3betaHSD/D activity; mutation to I: Lost activity.
6kvcA Moee5 in complex with udp-glucose and NAD (see paper)
29% identity, 67% coverage: 3:215/320 of query aligns to 3:207/299 of 6kvcA
- active site: S109 (= S118), S110 (≠ I119), S111 (≠ K120), Y132 (= Y144), K136 (= K148)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D31 (≠ A31), R32 (≠ F32), R33 (= R33), D46 (≠ E46), L47 (≠ R47), L65 (≠ C72), A66 (= A73), A67 (= A74), P69 (≠ V76), A107 (≠ L116), S109 (= S118), K136 (= K148), F161 (≠ P171), T163 (≠ L173), V164 (= V174), R170 (≠ K180), M173 (vs. gap)
- binding uridine-5'-diphosphate-glucose: P69 (≠ V76), R72 (≠ M79), S109 (= S118), S110 (≠ I119), Y132 (= Y144), T163 (≠ L173), M173 (vs. gap), F174 (= F183), R177 (vs. gap), E189 (≠ P197), I190 (≠ L198), Y191 (≠ G199), Q196 (≠ N204), R198 (= R206)
Sites not aligning to the query:
6x3bA Structure of rmd from pseudomonas aeruginosa complexed with NADPH
26% identity, 98% coverage: 3:317/320 of query aligns to 8:296/300 of 6x3bA
- active site: S108 (= S118), G109 (≠ I119), D110 (≠ K120), Y134 (= Y144), K138 (= K148)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G7), S14 (= S9), G15 (= G10), F16 (= F11), V17 (= V12), D42 (≠ E43), L43 (≠ R44), L63 (≠ C72), A64 (= A73), G65 (≠ A74), T67 (≠ V76), Y134 (= Y144), K138 (= K148), H164 (≠ V174), Q169 (≠ V179)
6x3bB Structure of rmd from pseudomonas aeruginosa complexed with NADPH
26% identity, 98% coverage: 3:317/320 of query aligns to 4:293/297 of 6x3bB
- active site: S104 (= S118), G105 (≠ I119), D106 (≠ K120), Y130 (= Y144), K134 (= K148)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), S10 (= S9), G11 (= G10), F12 (= F11), V13 (= V12), D38 (≠ E43), L39 (≠ R44), L59 (≠ C72), A60 (= A73), G61 (≠ A74), T63 (≠ V76), I102 (≠ L116), Y130 (= Y144), K134 (= K148), H160 (≠ V174), Q165 (≠ V179)
- binding pyrophosphate 2-: D106 (≠ K120), R199 (= R205)
Q9SYM5 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1; Protein REPRESSOR OF LRX1 1; Rhamnose biosynthetic enzyme 1; AtRHM1; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
24% identity, 99% coverage: 2:317/320 of query aligns to 8:319/669 of Q9SYM5
- R283 (= R282) mutation to K: In rol1-2; Abolishes dehydratase activity in vitro (PubMed:16766693). Induces aberrant accumulation of flavonols leading to alterations in plant growth and cell shape formation (PubMed:18567791, PubMed:18757557).
6zllA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-galacturonic acid and NAD (see paper)
23% identity, 100% coverage: 1:319/320 of query aligns to 1:312/321 of 6zllA
- active site: T126 (≠ S118), S127 (≠ I119), S128 (≠ K120), Y149 (= Y144), K153 (= K148)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D32 (≠ F32), H33 (≠ R33), F34 (vs. gap), I35 (vs. gap), K43 (≠ T38), D62 (vs. gap), I63 (vs. gap), L81 (≠ C72), A82 (= A73), A83 (= A74), I124 (≠ L116), T126 (≠ S118), Y149 (= Y144), K153 (= K148), Y176 (≠ P171), V179 (= V174), R185 (≠ K180), M188 (vs. gap)
- binding (2S,3R,4S,5R,6R)-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4,5-trihydroxy-oxane-2-carboxylic acid: P85 (≠ H77), V87 (≠ M79), R88 (≠ N80), T126 (≠ S118), S127 (≠ I119), Y149 (= Y144), T178 (≠ L173), R185 (≠ K180), A189 (vs. gap), R192 (≠ S185), T204 (≠ P197), F206 (≠ G199), Q211 (≠ N204), R213 (= R206), I250 (≠ T242), E276 (vs. gap)
6zldA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-glucuronic acid and NAD (see paper)
23% identity, 100% coverage: 1:319/320 of query aligns to 1:312/314 of 6zldA
- active site: T126 (≠ S118), S127 (≠ I119), S128 (≠ K120), Y149 (= Y144), K153 (= K148)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D32 (≠ F32), H33 (≠ R33), F34 (vs. gap), I35 (vs. gap), K43 (≠ T38), D62 (vs. gap), I63 (vs. gap), L81 (≠ C72), A82 (= A73), A83 (= A74), I124 (≠ L116), T126 (≠ S118), K153 (= K148), Y176 (≠ P171), T178 (≠ L173), R185 (≠ K180), M188 (vs. gap)
- binding uridine-5'-diphosphate-glucuronic acid: P85 (≠ H77), R88 (≠ N80), T126 (≠ S118), S127 (≠ I119), S128 (≠ K120), Y149 (= Y144), F177 (≠ V172), T178 (≠ L173), R185 (≠ K180), M188 (vs. gap), A189 (vs. gap), R192 (≠ S185), T204 (≠ P197), F206 (≠ G199), Q211 (≠ N204), R213 (= R206), I250 (≠ T242), E276 (vs. gap)
6zl6A Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp and NAD (see paper)
23% identity, 100% coverage: 1:319/320 of query aligns to 1:312/314 of 6zl6A
- active site: T126 (≠ S118), S127 (≠ I119), S128 (≠ K120), Y149 (= Y144), K153 (= K148)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D32 (≠ F32), H33 (≠ R33), F34 (vs. gap), I35 (vs. gap), K43 (≠ T38), D62 (vs. gap), I63 (vs. gap), L81 (≠ C72), A82 (= A73), A83 (= A74), I124 (≠ L116), T126 (≠ S118), K153 (= K148), Y176 (≠ P171), T178 (≠ L173), V179 (= V174), R185 (≠ K180), M188 (vs. gap)
- binding uridine-5'-diphosphate: T178 (≠ L173), A189 (vs. gap), R192 (≠ S185), T204 (≠ P197), F206 (≠ G199), Q211 (≠ N204), R213 (= R206), I250 (≠ T242), E276 (vs. gap)
7kn1A Crystal structure of udp-glucose-4-epimerase (gale) from stenotrophomonas maltophila with bound NAD and formylated udp- arabinopyranose
32% identity, 61% coverage: 1:195/320 of query aligns to 3:198/336 of 7kn1A
- active site: S126 (= S118), Y150 (= Y144), K154 (= K148)
- binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (≠ V12), D33 (≠ G30), S34 (≠ A31), C36 (≠ R33), N37 (≠ P34), D60 (= D58), I61 (≠ W59), F82 (≠ C72), A83 (= A73), A84 (= A74), K86 (≠ H77), S124 (≠ L116), S125 (= S117), S126 (= S118), Y150 (= Y144), K154 (= K148), Y178 (= Y175), P181 (≠ G178)
- binding UDP-4-deoxy-4-formamido-beta-L-arabinopyranose: V88 (≠ M79), S126 (= S118), Y150 (= Y144), N180 (≠ P177)
Sites not aligning to the query:
- binding UDP-4-deoxy-4-formamido-beta-L-arabinopyranose: 199, 200, 201, 217, 218, 219, 232, 234, 270, 293, 296
6zljA Crystal structure of udp-glucuronic acid 4-epimerase y149f mutant from bacillus cereus in complex with udp-4-deoxy-4-fluoro-glucuronic acid and NAD (see paper)
22% identity, 100% coverage: 1:319/320 of query aligns to 1:312/314 of 6zljA
- active site: T126 (≠ S118), S127 (≠ I119), S128 (≠ K120), F149 (≠ Y144), K153 (= K148)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D32 (≠ F32), H33 (≠ R33), F34 (vs. gap), I35 (vs. gap), K43 (≠ T38), D62 (vs. gap), I63 (vs. gap), L81 (≠ C72), A82 (= A73), A83 (= A74), I124 (≠ L116), T126 (≠ S118), K153 (= K148), Y176 (≠ P171), T178 (≠ L173), V179 (= V174), R185 (≠ K180), M188 (vs. gap)
- binding (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid: P85 (≠ H77), R88 (≠ N80), T126 (≠ S118), S127 (≠ I119), S128 (≠ K120), F149 (≠ Y144), F177 (≠ V172), T178 (≠ L173), R185 (≠ K180), M188 (vs. gap), A189 (vs. gap), R192 (≠ S185), T204 (≠ P197), F206 (≠ G199), Q211 (≠ N204), R213 (= R206), I250 (≠ T242), E276 (vs. gap)
2hunA Crystal structure of hypothetical protein ph0414 from pyrococcus horikoshii ot3
23% identity, 99% coverage: 1:317/320 of query aligns to 2:306/329 of 2hunA
- active site: T125 (≠ S118), D126 (≠ I119), E127 (≠ K120), Y149 (= Y144), K153 (= K148)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (≠ V12), D34 (≠ L28), K35 (≠ R29), S40 (≠ P34), D60 (≠ L53), V61 (≠ D54), L80 (≠ C72), A81 (= A73), A82 (= A74), S99 (≠ I93), T125 (≠ S118), K153 (= K148), C176 (≠ P171), T177 (≠ V172), N178 (≠ L173), N179 (≠ V174)
Q9R1J0 Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating; EC 1.1.1.170 from Mus musculus (Mouse) (see 3 papers)
23% identity, 98% coverage: 5:319/320 of query aligns to 31:357/362 of Q9R1J0
- V53 (≠ L27) to D: in bpa; does not rescue the yeast mutant lacking the ortholog erg26
- A94 (= A73) to T: in bpa; does not rescue the yeast mutant lacking the ortholog erg26
- P98 (≠ H77) to L: in str; does not rescue the yeast mutant lacking the ortholog erg26
- V109 (≠ I93) to M: in str; rescues the yeast mutant lacking the ortholog erg26
Sites not aligning to the query:
- 103:362 natural variant: Missing (in bpa)
- 359:362 Prevents secretion from ER
3c1tB Binding of two substrate analogue molecules to dihydroflavonol 4- reductase alters the functional geometry of the catalytic site (see paper)
26% identity, 73% coverage: 3:235/320 of query aligns to 8:252/326 of 3c1tB
- binding 3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4h-chromen-4-one: S125 (= S118), S125 (= S118), A126 (vs. gap), N130 (≠ K120), T156 (≠ P140), A157 (≠ T141), Y160 (= Y144), F161 (≠ G145), L189 (= L173), P201 (≠ K192), S202 (≠ G193), T205 (≠ L196), H216 (≠ R205), I219 (≠ L208), Q224 (≠ N213)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S14 (= S9), G15 (= G10), F16 (= F11), I17 (≠ V12), R37 (= R33), K44 (vs. gap), D64 (≠ A62), L65 (= L63), V84 (= V78), A85 (≠ M79), T86 (≠ N80), M88 (≠ T82), T123 (≠ L116), S124 (= S117), K164 (= K148), P187 (= P171), T188 (≠ V172), L189 (= L173), V190 (= V174), S202 (≠ G193)
Query Sequence
>HSERO_RS02375 FitnessBrowser__HerbieS:HSERO_RS02375
MHILVTGASGFVGRALLSRLDSEGQHLLRGAFRPSSETVMDRERIERTVISGLDPETDWS
AALHNIEAVVHCAARVHVMNETAGDPLAEFRRINLEGSLALARQAQQAGVQRFIFLSSIK
VNGEQTSLGRPFTADDKPAPTDPYGISKMEAEQALLELGRTGKMQVVIIRPVLVYGPGVK
ANFLSMMRWLDKGMPLPLGGITHNRRSLVALDNLVDLITVCLAHPAAAGQVFLASDGEDL
STTGMLQRLGRALDKSPTLLPVPASWLTLAARLMGKKGIAQRLCGSLQVDISKNRELLGW
SPPITVDAAFRKTVDAYRRA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory