SitesBLAST

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
22% identity, 95% coverage: 8:430/447 of query aligns to 75:469/534 of P53322

query
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P53322

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K283 (≠ Q216) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
22% identity, 65% coverage: 66:356/447 of query aligns to 113:392/495 of Q9NRA2

query
sites
Q9NRA2

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K136 (≠ R89) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ V134) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AM 149:150) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ VI 225:226) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ AAEQE 227:231) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G289) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P295) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (≠ A335) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
23% identity, 74% coverage: 68:398/447 of query aligns to 90:430/493 of Q03567

query
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Q03567

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Sites not aligning to the query:

69 modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 69% coverage: 47:356/447 of query aligns to 94:392/495 of Q5Q0U0

query
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Q5Q0U0

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A

Q

I

L

A

G

S

T

I

V

L

I

S

S

G

L

P

P

I

L

T

S

G

G

A

I

L

I

L

C

L

Y

I

-

E

-

G

-

L

Y

H

M

G

N

W

W

Q

T

W

Y

M

V

F

F

I

Y

I

L

E

F

G

G

G

L

A

V

S

G

M

I

V

V

-

W

F

F

C

I

V

L

V

W

V

M

W

W

L

I

V

V

L

S

D

D

S

-

T

T

P

P

R

E

E

T

A

H

K

K

W

T

L

I

S

S

Q

H

E

Y

Q

E

R

K

E

E

A

-

L

-

A

-

A

Y

V

I

I

V

A
x
S

E
x
L

Q
x
K

E
x
N

Q

Q

R

L

A

S

N

Q

R

K

P

V

A

-

H

-

V

V

S

P

A

W

L

V

S

S

L

I

L

L

R

K

D

S

P

L

Q

P

I

L

V

W

L

A

F

I

C

V

F

V

I

A

Y

H

F

F

A

S

I

Y

Q

N

L

W

T

T

I

F

Y

Y

G

T

A

L

T

L

F

T

W

L

L

L

P

P

T

T

I

Y

I

M

K

K

K

E

M

I

G

L

H

R

F

F

N

N

D

V

F

Q

Q

E

V

N

G

G

L

F

F

L

N

S

S

A

I

L

P
|
P

W

Y

I

F

V

G

A

C

I

W

A

L

A

C

M

M

Y

-

L

I

F

V

A

C

V

G

L

Q

A

A

R

D

Y

Y

K

L

K

R

P

V

Q

K

F

-

W

W

V

N

A

F

T

S

T

T

L

I

V

S

I

V

S

R

A

R

A

I

G

-

M

F

A

S

I

L

S

I

A
x
G

V

M

A

V

G

G

P

P

V

A

I

V

G

F

F

L

V

V

A

A

V

A

C

G

F

F

A

I

G

G

I

C

G

D

F

Y

K

S

A

L

A

A

K136 (≠ R89) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R119) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L122) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G123) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E126) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A127) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (= F130) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P131) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ V134) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ F137) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ AAEQE 227:231) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P295) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (≠ A335) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
22% identity, 69% coverage: 47:356/447 of query aligns to 94:392/495 of Q8BN82

query
sites
Q8BN82

R

H

S

N

H

H

L

T

S

G

T

K

D

K

L

Y

G

K

I

W

G

D

A

A

A

E

A

T

F

Q

G

G

L

W

G

I

A

L

G

G

L

S

F

F

V

Y

G

G

Y

Y

A

I

L

V

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

I

L

A

Q

S

R

R

-

V

-

G

-

K

-

L

Y

L

G

G

A

L

R

G

A

I

W

L

L

G

T

T

R

S

I

V

M

F

F

T

T

L

W

F

G

T

L

P

V

L

A

A

C

D

M

L

A

G

F

V

V

V

Q

-

S

-

E

-

T

-

S

T

F

L

Y

V

F

V

M

L

R

R

F

A

L

L

L

E

G

G

V

L

A

G

E

E

A

G

G

V

F

T

F

F

P

P

G

A

V

M

V
x
H

Y

A

Y

M

F

W

T

S

Q

S

W

W

L

A

P

P

N

P

H

L

E

E

R

R

G

S

R

K

A

L

M

L

A

T

V

I

F

S

L

Y

S

A

G

G

S

A

A

Q

I

L

A

G

S

T

I

V

L

I

S

S

G

L

P

P

I

L

T

S

G

G

A

I

L

I

L

C

L

Y

I

-

E

-

G

-

L

Y

H

M

G

N

W

W

Q

T

W

Y

M

V

F

F

I

Y

I

L

E

F

G

G

G

I

A

V

S

G

M

I

V

V

-

W

F

F

C

I

V

L

V

W

V

M

W

W

L

I

V

V

L

S

D

D

S

-

T

T

P

P

R

E

E

T

A

H

K

K

W

T

L

I

S

S

Q

H

E

Y

Q

E

R

K

E

E

A

-

L

-

A

-

A

Y

V

I

I

V

A

S

E

L

Q

K

E

N

Q

Q

R

L

A

S

N

Q

R

K

P

V

A

-

H

-

V

V

S

P

A

W

L

G

S

S

L

I

L

L

R

K

D

S

P

L

Q

P

I

L

V

W

L

A

F

I

C

V

F

V

I

A

Y

H

F

F

A

S

I

Y

Q

N

L

W

T

S

I

F

Y

Y

G

T

A

L

T

L

F

T

W

L

L

L

P

P

T

T

I

Y

I

M

K

K

K

E

M

I

G

L

H

R

F

F

N

N

D

V

F

Q

Q

E

V

N

G

G

L

F

F

L

N

S

S

A

I

L

P

P

W

Y

I

F

V

G

A

C

I

W

A

L

A

C

M

M

Y

I

L

L

F

C

A

G

V

-

L

Q

A

A

R

D

Y

Y

K

L

K

R

P

V

Q

K

F

-

W

W

V

N

A

F

T

S

T

T

L

I

V

S

I

V

S

R

A

R

A

I

G

-

M

F

A

S

I

L

S

V

A

G

V

M

A

V

G

G

P

P

V

A

I

V

G

F

F

L

V

V

A

A

V

A

C

G

F

F

A

I

G

G

I

C

G

D

F

Y

K

S

A

L

A

A

H183 (≠ V134) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
21% identity, 76% coverage: 62:401/447 of query aligns to 125:470/582 of Q9JI12

query
sites
Q9JI12

G

G

L

M

G

I

A
x
H

G

G

L

S

F

F

V

W

G

G

Y

Y

A

I

L

I

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

I

L

A

Q

S

R

R

Y

L

G

A

A

A

-

N

R

R

A

V

W

F

L

G

T

A

R

A

I

I

M

L

F

L

T

T

W

S

G

T

L

L

V

-

A

-

C

N

M

M

A

L

F

I

V

P

Q

S

S

A

E

A

T

R

S

V

F

H

Y

Y

-

G

-

C

-

V

-

I

F

F

M

V

R
|
R

F

I

L

L

L

Q

G

G

V

L

A

V

E
|
E

A

G

G

V

F

T

F

Y

P

P

G

A

V

C

V

H

Y

G

Y

I

F

W

T

S

Q

K

W

W

L

A

P

P

N

P

H

L

E

E

R

R

G

S

R

R

A

L

M

A

A

T

V

T

F

S

L

F

S

C

G

G

S

S

A

Y

I

A

A

G

S

A

I

V

L

I

S

A

G

M

P

P

I

L

T

A

G

G

A

I

L

L

L

V

L

Q

I

-

E

-

G

-

L

Y

H

T

G

G

W

W

Q

S

W

S

M

V

F

F

I

Y

I

V

E

Y

G

G

G

S

A

F

S

G

M

M

V

V

F

W

C

Y

V

M

V

F

V

W

W

L

L

L

V

V

L

S

D

Y

S

E

T

S

P

P

R

A

E

K

A

H

K

P

W

T

L

I

S

T

Q

D

E

E

Q

E

R

R

E

R

A

Y

L

I

A

E

V

S

I

I

A

G

A

E

E

S

Q

A

E

N

Q

L

R

L

A

G

A

A

N

M

R

E

P

K

A

F

H

K

V

T

S

P

A

W

L

R

S

K

L

F

R

T

D

S

P

M

Q

P

I

V

V

Y

L

A

F

I

C

I

F

V

I

A

Y

N

F

F

A

C

I
x
R

Q

S

L

W

T

T

I

F

Y

Y

G

L

A

L

T

L

F

I

W

S

L

Q

P

P

T

A

I

Y

I

F

K

E

M

V

G

F

H

G

F

F

N

E

D

I

F

S

Q

K

V

V

G

G

L

M

F

L

N

S

S

A

I

V

P

P

W

H

I

L

V

V

A

M

I

T

A

I

M

V

Y

P

L

I

F

G

A

G

V

Q

L

I

A

A

A

D

R

F

Y

L

K

R

K

S

P

K

Q

Q

F

I

W

L

V

S

A

T

T

T

L

V

-

R

-

K

V

I

I

M

S

N

A

C

A

G

G

G

M

F

A

G

I

M

S

E

A

A

V

T

A

L

G

L

P

L

V

V

I

V

G

G

F

Y

-

S

-

H

-

T

-

R

-

G

V

V

A

A

V

I

C

S

F

F

-

L

-

V

A

L

G

A

I

V

G

G

F

F

K

S

A

G

A

F

S

A

S

I

L

S

F

G

W

F

P

N

I

V

P

N

Q

H

A

L

Y

D

L

I

D

A

A

P

R

R

I

Y

A

A

A

S

A

I

V

L

L

M

A

G

L

I

I

S

N

N

S

G

L

V

G

G

N

T

L

L

G

S

G

G

F

M

F

V

A

C

P

P

A

I

T

I

F

V

G

G

Y

A

L

M

E

T

Q

K

K

N

H128 (≠ A65) mutation to A: Greatly lowers L-glutamate transport.
R184 (= R119) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E126) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ I262) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
20% identity, 68% coverage: 19:320/447 of query aligns to 92:401/570 of Q9C0U9

query
sites
Q9C0U9

S

S

K

K

I

L

K

K

W

W

R

K

I

L

L

Y

P

I

L

Y

F

L

V

L

A

L

M

M

F

L

-

G

-

F

-

L

-

D

I

M

N

L

Y

F

I

I

D

G

R

K

V

A

N

T

I

L

G

S

F

Y

V

S

R

T

S

I

-

L

H

G

L

L

S

F

T

D

D

D

L

V

G

H

I

I

G

T

A

S

A

N

A

Q

F

Y

G

N

L

N

G

L

A

N

G

T

L

L

F

F

F

Y

V

V

G

G

Y

Y

A

I

L

V

F

G

E

Q

V

F

P

P

S

G

N

H

M

Y

L

I

L

M

Q

Q

R

T

Y

F

G

P

A

L

R

G

A

K

W

F

L

V

T

G

R

L

I

V

M

T

F

F

T

S

W

W

G

S

L

V

V

I

A

V

A

F

C

L

M

H

A

C

F

C

V

A

Q

Y

S

N

E

Y

T

G

S

G

F

L

Y

I

F

A

M

L

R

R

F

F

L

F

L

L

G

G

V

F

A

T

E

E

A

S

G

C

F

L

P

P

G

A

V

M

V

E

Y

A

Y

T

F

M

T

G

Q

M

W

F

L

F

P

T

N

H

H

Q

E

E

R

Q

G

A

R

F

A

L

M

Q

A

P

V

V

F

F

L

W

S

I

G

S

S

C

A

L

I

S

A

C

S

G

I

I

L

P

S

A

G

G

P

F

I

I

T

A

G

Y

A

G

L

L

L

E

L

F

I

V

E

T

G

-

G

K

G

S

L

I

H

A

G

P

W

W

Q

K

W

L

M

F

F

M

I

I

E

T

G

G

A

I

S

T

M

F

V

F

F

L

C

S

V

I

V

F

V

L

W

F

L

F

V

Y

L

Y

D

P

S

D

T

N

P

P

R

S

E

K

A

A

K

R

W

F

L

L

S

T

Q

D

E

E

Q

E

R

K

-

L

-

Y

-

T

-

I

E

D

A

R

L

V

A

R

A

K

V

S

I

T

A

R

A

G

E

G

Q

I

E

E

Q

N

R

K

A

I

A

F

N

K

R

K

P

H

A

Q

H

F

V

I

S

E

A

A

L

-

S

-

L

-

L

L

R

K

D

D

P

P

Q

I

I

T

V

W

L

L

F

F

C

T

F

F

I

A

Y

A

F

F

A

T

I

L

Q

M

L

L

T

S

-

N

-

L

I

A

Y

Y

G

Q

A

Q

T

N

F

L

W

I

L

F

P

T

T

S

I

L

I

-

K

-

K

N

M

V

G

S

H

D

F

L

N

N

D

S

F

T

Q

L

V

V

G

G

L

V

-

A

-

L

-

A

-

G

F

Y

N

N

S

T

I

V

P

S

W

A

I

I

V

I

A

A

I

T

A

F

A

A

M

M

Y

Y

L

L

F

I

A

P

V

N

L

Q

A

S

A

A

R

Y

Y

H

K

-

P

A

Q

M

F

F

W

W

V

M

Sites not aligning to the query:

14 modified: Phosphoserine

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
23% identity, 58% coverage: 22:281/447 of query aligns to 20:285/452 of Q5EXK5

query
sites
Q5EXK5

K

Q

W

W

R

R

I

V

L

I

P

I

L

C

F

C

V

F

A

L

M

V

F

V

I

M

I

L

N

D

Y

G

I

F

D

D

R

T

V

A

N

A

I

I

G

G

F

F

V

I

-

A

-

P

-

D

-

I

R

R

S

S

H

H

L

W

S

Q

T

L

D

T

L

A

G

G

I

D

G

L

A

A

A

P

A

L

F

F

G

G

L

A

G

G

A

L

G

-

L

-

F

-

V

L

G

G

Y

L

A

T

L

A

F

G

E

A

V

L

P

L

S

C

N

G

M

P

L

L

L

S

Q
x
D

R

R

Y

F

G

G

A

R

R

K

A

R

W

V

L

I

T

E

R

F

I

C

M

V

F

A

T

L

W

F

G

G

L

L

V

F

A

S

A

L

C

L

M

S

A

A

F

F

V

A

Q

P

S

D

E

L

T

Q

S

M

F

L

Y

V

F

F

M

L

R

R

F

F

L

L

L

T

G

G

V

L

A

G

E

L

A

G

G

G

F

A

F

M

P

P

G

N

V

T

V

I

Y

T

Y

M

F

T

T

S

Q

E

W

Y

L

L

P

P

N

A

H

R

E

R

R

R

G

G

R

A

A

L

M

V

A

T

V

L

F

M

L

F

S

C

G

G

S

F

A

T

I

L

A

G

S

S

I

A

L

F

S

G

G

G

P

V

I

V

T

S

G

A

A

Q

L

L

L

V

L

-

I

-

E

-

G

-

G

P

L

L

H

I

G

G

W

W

Q

H

W

G

M

I

F

L

I

V

I

L

E

G

G

G

G

V

A

L

S

P

M

L

V

I

F

L

C

A

V

V

V

A

V

L

W

V

L

P

V

L

L

L

D

P

S

E

T

S

P

P

R

R

-

W

-

Q

E

I

A

R

K

R

W

Q

L

L

S

P

Q

Q

E

A

Q

T

R

I

E

A

A

R

L

T

A

V

A

G

V

A

I

I

A

T

A

G

E

E

Q

R

-

Y

-

N

-

D

-

T

-

H

-

F

-

W

-

L

E

D

Q

E

R

P

A

A

N

G

R

A

P

K

A

G

H

S

V

I

S

S

A

Q

L

L

S

F

L

A

L

G

R

R

D

Q

P

L

Q

A

I

I

V

T

L

L

F

M

C

L

F

W

I

V

Y

V

F

F

A

F

I

M

Q

S

-

L

L

L

T

I

I

I

Y

Y

G

L

A

L

T

S

F

S

W

W

L

M

P

P

T

T

I

L

K

N

K

H

M

R

G

G

D82 (≠ Q84) mutation to A: Loss of activity.

Sites not aligning to the query:

311 V→W: Loss of activity.
314 D→A: Loss of activity.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
21% identity, 76% coverage: 62:401/447 of query aligns to 67:412/452 of 7t3nA

query
sites
7t3nA

G

G

L

M

G

I

A

H

G

G

L

S

F

F

V

W

G

G

Y
|
Y

A

I

L

I

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

I

L

A

Q

S

R

R

-

L

-

A

-

N

-

R

-

V

Y

F

G

G

A

A

R

A

A

I

W

L

L

L

T

T

R

S

I

T

M

L

F

N

T

M

W

L

G

I

L

P

V

S

A

A

-

R

-

V

-

H

-

Y

-

G

C

C

M

V

A

I

F

F

V

V

Q

Q

S

-

E

-

T

-

S

-

F

-

Y

-

F

-

M

-

R

-

F

I

L

L

L

Q

G

G

V

L

A

V

E

Q

A

G

G

V

F

T

F
x
Y

P

P

G

A

V

C

V

H

Y

G

Y

I

F

W

T

S

Q

K

W

W

L

A

P

P

N

P

H

L

E
|
E

R

R

G

S

R

R

A

L

M

A

A

T

V

T

F

S

L

F

S

C

G
|
G

S

S

A
x
Y

I

A

A

G

S

A

I

V

L

I

S

A

G

M

P

P

I

L

T

A

G

G

A

I

L

L

L

V

L

Q

I

Y

E

T

G

G

G

-

L

-

H

-

G

-

W

W

Q

S

W

S

M

V

F

F

I

Y

I

V

E

Y

G

G

G

S

A

F

S

G

M

M

V

V

F

W

C

Y

V

M

V

F

V

W

W

L

L

L

V

V

L

S

D

Y

S

E

T

S

P

P

R

A

E

K

A

H

K

P

W

T

L

I

S

T

Q

D

E

E

Q

E

R

R

E

R

A

Y

L

I

A

E

V

S

I

I

A

G

A

E

E

S

Q

A

E

N

Q

L

R

L

A

G

A

A

N

M

R

E

P

K

A

F

H

K

V

T

S

P

A

W

L

R

S

K

L

F

R

T

D

S

P

M

Q

P

I

V

V

Y

L

A

F

I

C

I

F

V

I

A

Y

N

F

F

A

C

I
x
R

Q

S

L

W

T

T

I
x
F

Y
|
Y

G

L

A

L

T

L

F

I

W

S

L

Q

P

P

T

A

I

Y

I

F

K

E

M

V

G

F

H

G

F

F

N

E

D

I

F

S

Q

K

V

V

G

G

L

M

F

L

N

S

S

A

I

V

P

P

W

H

I

L

V

V

A

M

I

T

A

I

M

V

Y

P

L

I

F

G

A

G

V

Q

L

I

A

A

A

D

R

F

Y

L

K

R

K

S

P

K

Q

Q

F

I

W

L

V

S

A

T

T

T

L

V

-

R

-

K

V

I

I

M

S

N

A

C

A

G

G

G

M

F

A

G

I

M

S

E

A

A

V

T

A

L

G

L

P

L

V

V

I

V

G

G

F

Y

-

S

-

H

-

T

-

R

-

G

V

V

A

A

V

I

C

S

F

F

-

L

-

V

A

L

G

A

I

V

G

G

F

F

K

S

A

G

A

F

S

A

S

I

L

S

F

G

W

F

P

N

I

V

P

N

Q

H

A

L

Y

D

L

I

D

A

A

P

R

R

I

Y

A

A

A

S

A

I

V

L

L

M

A

G

L

I

I

S

N

N

S

G

L

V

G

G

N

T

L

L

G

S

G

G

F

M

F

V

A

C

P

P

A

I

T

I

F

V

G

G

Y

A

L

M

E

T

Q

K

K

N

binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y72), Y137 (≠ F130), Y165 (≠ A158), R264 (≠ I262), F268 (≠ I266), Y269 (= Y267)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E145), G163 (= G156)

Sites not aligning to the query:

binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: 12, 13

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
21% identity, 63% coverage: 1:281/447 of query aligns to 1:294/448 of Q51955

query
sites
Q51955

V

M

N

N

P

Q

S

A

Q

Q

S

N

S

S

T

V

P

G

A

K

G

S

L

L

A

D

L

V

T

Q

S

S

-

F

-

I

-

N

-

Q

A

P

V

L

S

S

K

R

I

Y

K

Q

W

W

R

R

I

V

L

V

P

L

L

L

F

C

V

F

A

L

M

I

F

V

I

F

I

L

N
x
D

Y

G

I

L

D
|
D

R

T

V

A

N

A

I

M

G

G

F

F

V

I

R

A

S

P

H

A

L

L

S

S

T

Q

D

E

L

W

G

G

I

I

G

D

A

R

A

A

A

S

F

L

G

G

L

P

G

V

A

M

G

S

L

A

F

A

F

L

V

I

G

G

Y

M

A

V

L

F

F

G

E

A

V

L

P

G

S

S

N
x
G

M

P

L

L

L

A

Q
x
D

R

R

Y

F

G
|
G

A

R

R

K

A

G

W

V

L

L

T

V

R

G

I

A

M

V

F

L

T

V

W

F

G

G

L

G

V

F

A

S

A

L

C

A

M

S

A

A

F

Y

V

A

Q

T

S

N

E

V

T

D

S

Q

F

L

Y

L

F

V

M

L

R
|
R

F

F

L

L

L

T

G

G

V

L

A

G

E

L

A

G

G

A

F

G

F

M

P

P

G

N

V

A

V

T

Y

T

Y

L

F

L

T

S

Q
x
E

W

Y

L

T

P

P

N

E

H

R

E

L

R

K

G

S

R

L

A

L

M

V

A

T

V

S

F

M

L

F

S

C

G

G

S

F

A

N

I

L

A

G

S

M

I

A

L

G

S

G

G

G

P

F

I

I

T

S

G

A

A

K

L

M

L

I

L

P

I

A

E

-

G

-

L

-

H

Y

G

G

W

W

Q
x
H

W

S

M

L

F

L

I

V

I

I

E

G

G

G

G

V

A

L

S

P

M

L

V

L

F

L

C

A

V

L

V

V

V

-

W

-

L

-

V

L

L

M

D

V

S

W

T

L

P

P

R

E

E

S

A

A

K

R

W

F

L

L

-

V

-

R

-

N

-

R

-

G

S

T

Q

D

E

K

Q

I

R

R

E

K

A

T

L

L

A

S

A

P

V

I

-

A

-

P

-

Q

I

V

A

V

A

A

E

E

Q

A

-

G

-

S

-

F

-

S

-

V

-

P

E

E

Q

Q

R

K

A

A

-

V

A

A

N

A

R

R

P

S

A

V

H

F

V

A

S

V

A

I

L

F

S

S

L

G

L

T

R

Y

D

G

P

L

Q

G

I

T

V

M

L

L

F

L

C

W

F

L

I

T

Y

Y

F

F

A

M

I

G

Q

L

L

V

T

I

I

V

Y

Y

G

L

A

L

T

T

F

S

W

W

L

L

P

P

T

T

I

L

I

M

K

R

K

D

M

S

G

G

D41 (≠ N36) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D39) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (≠ N80) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (≠ Q84) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G87) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R119) mutation to A: Abolishes 4-HBA transport.
E144 (≠ Q139) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ Q183) mutation to A: Decrease in 4-HBA transport and chemotaxis.

Sites not aligning to the query:

323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
23% identity, 63% coverage: 2:282/447 of query aligns to 21:284/444 of Q8NLB7

query
sites
Q8NLB7

N

N

P

S

S

S

Q

Q

S

T

S

I

T

E

P

N

A

K

G

G

L

L

A

T

L

I

T

L

S

G

A

I

V

S

S

G

K

R

I

-

K

-

W

-

R

R

I

L

L

A

P

A

L

V

F

L

V

I

A

G

M

W

F

F

-

F

-

V

I

I

I

F

N
x
D

Y

G

I

Y

D
|
D

R

L

V

I

N

V

I

Y

G

G

F

T

V

V

R

Q

S

S

H

A

L

L

S

A

T

K

D

E

L

W

G

N

I

L

G

S

A

S

A

A

A

T

F

L

G

G

L

T

G

I

A

G

G

S

L

T

F

A

F

F

V

F

G

G

Y

M

A

A

L

I

F

G

E

A

V

V

P

F

S

I

N

G

M

R

L

L

L

S

Q

D

R
|
R

Y

V

G

G

A

R

R

K

A

A

W

A

L

V

T

I

R

G

I

S

M

V

F

L

T

I

W

L

G

S

L

V

V

F

A

T

A

M

C

L

M

C

A

A

F

F

V

A

Q

P

S

N

E

P

T

W

S

V

F

F

Y

G

F

A

M

F

R

R

F

F

L

I

L

A

G

G

V

L

A

G

E

L

A

G

G

G

F

L

F

V

P

P

G

S

V

V

V

N

Y

A

Y

M

F

T

T

S

Q

D

W

L

L

V

P

P

N

R

H

K

E

T

R

M

G

S

R

A

A

W

M

A

A

T

V

V

F

M

L

M

S

S

G

G

S

V

A

P

I

I

A

G

S

G

I

S

L

I

S

A

G

A

P

V

I

L

T

-

G

-

A

A

L

L

L

V

I

V

E

P

G

S

G

E

L

E

H

W

G

G

W

W

Q

R

W

F

M

M

F

F

I

L

I

I

E

-

G

-

A

-

S

A

M

L

V

I

F

P

C

L

V

V

V

-

W

-

L

-

V

-

L

-

D

-

S

G

T

L

P

P

R
</