SitesBLAST
Comparing HSERO_RS08375 FitnessBrowser__HerbieS:HSERO_RS08375 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
24% identity, 93% coverage: 3:405/433 of query aligns to 4:348/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ L6), G8 (= G7), G10 (= G9), V11 (= V10), I12 (= I11), V30 (≠ L29), E31 (≠ D30), K32 (≠ R31), E38 (= E38), A39 (≠ T39), S40 (= S40), A43 (≠ N43), G45 (= G45), L46 (≠ Q46), V171 (≠ I224), G200 (≠ L253), G201 (= G254), W203 (≠ Y256), G298 (= G356), R300 (= R358), P301 (= P359), Y326 (≠ G383), R327 (≠ T384), N328 (≠ L385), G329 (= G386), I330 (≠ W387)
2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
22% identity, 76% coverage: 91:418/433 of query aligns to 59:387/403 of 2gagB
- active site: A61 (≠ L93), T64 (≠ C96), T65 (≠ N97)
- binding flavin-adenine dinucleotide: N59 (≠ Q91), M60 (= M92), R62 (= R94), N63 (= N95), T64 (≠ C96), I66 (≠ A98), V195 (≠ I224), G224 (≠ L253), A225 (≠ G254), H227 (≠ Y256), L231 (= L261), L246 (≠ I280), G352 (= G383), T353 (= T384), G354 (≠ L385), G355 (= G386), F356 (≠ W387), K357 (≠ T388)
- binding flavin mononucleotide: A61 (≠ L93), R62 (= R94), H171 (≠ C200), V250 (≠ I284), E278 (≠ G315), R321 (≠ S352), W323 (= W354)
- binding 2-furoic acid: T64 (≠ C96), I66 (≠ A98), R68 (= R100), M263 (≠ E298), Y270 (≠ F307), K357 (≠ T388)
- binding sulfite ion: K170 (≠ D199), K276 (≠ V313)
Sites not aligning to the query:
6j39A Crystal structure of cmis2 with inhibitor (see paper)
29% identity, 76% coverage: 92:418/433 of query aligns to 44:368/368 of 6j39A
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: M44 (= M92), P46 (≠ R94), N49 (= N97), R243 (≠ S293), Y252 (≠ I302), Y267 (≠ A317), R308 (= R358), R334 (≠ T384), I335 (≠ L385)
- binding flavin-adenine dinucleotide: M44 (= M92), A174 (= A228), A203 (≠ L253), W206 (≠ Y256), I228 (≠ S279), Y252 (≠ I302), R308 (= R358), S333 (≠ G383), R334 (≠ T384), I335 (≠ L385), G336 (= G386), V337 (≠ W387), Q338 (≠ T388)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 7, 9, 10, 11, 29, 30, 31, 32, 36, 37, 38, 40, 41, 42, 43
6j38A Crystal structure of cmis2 (see paper)
29% identity, 76% coverage: 92:418/433 of query aligns to 44:368/368 of 6j38A
- binding flavin-adenine dinucleotide: M44 (= M92), A174 (= A228), A203 (≠ L253), W206 (≠ Y256), G226 (= G277), G306 (= G356), R308 (= R358), S333 (≠ G383), R334 (≠ T384), I335 (≠ L385), G336 (= G386), V337 (≠ W387), Q338 (≠ T388)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 7, 9, 11, 29, 30, 31, 36, 37, 38, 41, 42, 43
1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
23% identity, 70% coverage: 100:402/433 of query aligns to 51:352/374 of 1y56B
- active site: H224 (≠ G277), P239 (= P291), G305 (= G356), M338 (≠ T388)
- binding flavin-adenine dinucleotide: E170 (≠ D223), V171 (≠ I224), T200 (≠ L253), N201 (≠ G254), W203 (≠ Y256), G305 (= G356), Y306 (≠ L357), Y307 (≠ R358), G334 (≠ T384), H335 (≠ L385), G336 (= G386), F337 (≠ W387), M338 (≠ T388)
- binding flavin mononucleotide: I260 (≠ R312), R301 (≠ S352), W303 (= W354)
Sites not aligning to the query:
- active site: 44, 47, 48
- binding flavin-adenine dinucleotide: 11, 13, 14, 15, 33, 34, 35, 42, 43, 45, 46, 47, 49
- binding flavin mononucleotide: 44, 45
6pxsA Crystal structure of iminodiacetate oxidase (idaa) from chelativorans sp. Bnc1 (see paper)
27% identity, 97% coverage: 1:422/433 of query aligns to 1:367/370 of 6pxsA
- binding flavin-adenine dinucleotide: G7 (= G7), G9 (= G9), I10 (≠ V10), D30 (= D30), N32 (vs. gap), H33 (≠ Q32), K36 (≠ P35), A37 (= A36), T38 (≠ S40), A40 (≠ G42), G41 (≠ N43), A42 (= A44), G43 (= G45), V44 (≠ Q46), Y174 (≠ I224), A203 (≠ L253), W206 (≠ Y256), I210 (≠ L260), Y250 (= Y300), G305 (= G356), R307 (= R358), G333 (= G383), A334 (≠ T384), S335 (≠ L385), G336 (= G386), L337 (≠ W387), T338 (= T388)
P40875 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 3 papers)
21% identity, 76% coverage: 91:418/433 of query aligns to 61:389/405 of P40875
- C146 (≠ E176) mutation to S: No change in activity.
- H173 (≠ C200) modified: Tele-8alpha-FMN histidine; mutation to N: Prevents covalent attachment of FMN. Loss of activity. The mutant is considerably less stable than wild-type enzyme, the beta and delta subunits are lost during purification, which yields a stable alpha-gamma complex.
- H175 (≠ L202) mutation to A: No effect on FMN binding and activity.
- C195 (≠ V222) mutation to S: No change in activity.
- C351 (≠ T380) mutation to A: No change in activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 30 G→A: Prevents covalent attachment of FMN. Blocks subunit assembly.
Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
25% identity, 73% coverage: 108:423/433 of query aligns to 62:375/377 of Q5L2C2
- V180 (≠ D223) binding
- R309 (= R358) binding
- 334:340 (vs. 382:388, 29% identical) binding
- R336 (≠ T384) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
25% identity, 72% coverage: 108:419/433 of query aligns to 61:369/370 of 4yshA
- active site: I262 (≠ V313), L283 (= L334), G305 (= G356), N335 (≠ L385), L338 (≠ T388)
- binding flavin-adenine dinucleotide: V178 (≠ D223), S206 (≠ L253), G207 (= G254), W209 (≠ Y256), R307 (= R358), H332 (= H382), R334 (≠ T384), N335 (≠ L385), G336 (= G386), I337 (≠ W387)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
23% identity, 97% coverage: 1:418/433 of query aligns to 26:400/824 of Q8GAI3
- W66 (= W55) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- H67 (≠ A56) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
25% identity, 72% coverage: 108:418/433 of query aligns to 61:368/368 of 4yshB
- active site: I262 (≠ V313), L283 (= L334), G305 (= G356), N335 (≠ L385), L338 (≠ T388)
- binding flavin-adenine dinucleotide: V178 (≠ D223), S206 (≠ L253), W209 (≠ Y256), R307 (= R358), H332 (= H382), R334 (≠ T384), N335 (≠ L385), G336 (= G386), I337 (≠ W387), L338 (≠ T388)
- binding glycine: G249 (≠ Y300), Y251 (≠ I302), Y251 (≠ I302), A264 (≠ G315), R307 (= R358), R334 (≠ T384), R334 (≠ T384)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48
Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
22% identity, 66% coverage: 134:418/433 of query aligns to 104:389/405 of Q50LF2
- K172 (≠ D199) mutation to A: Retains 39% of wild-type activity.; mutation to D: Retains 32% of wild-type activity.; mutation to R: Retains 58% of wild-type activity.
- H173 (≠ C200) modified: Tele-8alpha-FMN histidine
- V197 (≠ I224) binding
- H270 (≠ A303) mutation to A: 10-fold decrease in catalytic efficiency.
- Y272 (≠ F307) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
- G354 (= G383) binding
- G357 (= G386) binding
- K359 (≠ T388) binding ; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.
Sites not aligning to the query:
- 31 binding
- 32 binding
- 53 binding
- 61 binding
- 62 binding
- 66 binding
- 68 binding
1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
22% identity, 66% coverage: 134:418/433 of query aligns to 103:388/402 of 1vrqB
- active site: G326 (= G356), K358 (≠ T388)
- binding n,n-dimethylglycine: K358 (≠ T388)
- binding flavin-adenine dinucleotide: V196 (≠ I224), A224 (= A252), G225 (≠ L253), H228 (≠ Y256), L247 (≠ I280), G353 (= G383), T354 (= T384), G355 (≠ L385), G356 (= G386), F357 (≠ W387), K358 (≠ T388)
- binding flavin mononucleotide: H172 (≠ C200), V251 (≠ I284), E279 (≠ G315), R322 (≠ S352), W324 (= W354)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding n,n-dimethylglycine: 65, 67, 69, 401
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 51, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
22% identity, 66% coverage: 134:418/433 of query aligns to 103:388/404 of 3ad8B
- active site: G326 (= G356), K358 (≠ T388)
- binding flavin-adenine dinucleotide: V196 (≠ I224), G225 (≠ L253), A226 (≠ G254), H228 (≠ Y256), L247 (≠ I280), G353 (= G383), T354 (= T384), G355 (≠ L385), G356 (= G386), F357 (≠ W387), K358 (≠ T388)
- binding flavin mononucleotide: H172 (≠ C200), V251 (≠ I284), E279 (≠ G315), R322 (≠ S352), W324 (= W354)
- binding pyrrole-2-carboxylate: M264 (≠ E298), Y271 (≠ F307), T354 (= T384), K358 (≠ T388)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
- binding pyrrole-2-carboxylate: 65, 67, 69, 402
3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
22% identity, 66% coverage: 134:418/433 of query aligns to 103:388/404 of 3ad7B
- active site: G326 (= G356), K358 (≠ T388)
- binding flavin-adenine dinucleotide: V196 (≠ I224), G225 (≠ L253), A226 (≠ G254), H228 (≠ Y256), L247 (≠ I280), G353 (= G383), T354 (= T384), G355 (≠ L385), G356 (= G386), F357 (≠ W387), K358 (≠ T388)
- binding flavin mononucleotide: H172 (≠ C200), V251 (≠ I284), K277 (≠ V313), E279 (≠ G315), R322 (≠ S352), W324 (= W354)
- binding [methylthio]acetate: M264 (≠ E298), Y271 (≠ F307), T354 (= T384), K358 (≠ T388)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
- binding [methylthio]acetate: 67, 69, 402
1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
21% identity, 96% coverage: 2:418/433 of query aligns to 4:391/828 of 1pj6A
- active site: H223 (≠ K276), Y257 (≠ A303)
- binding flavin-adenine dinucleotide: G9 (= G7), G11 (= G9), I12 (≠ V10), V13 (≠ I11), D33 (= D30), Q34 (≠ R31), G42 (= G42), S43 (≠ N43), T44 (≠ A44), H46 (≠ Q46), P48 (= P58), L50 (≠ I60), V172 (≠ I224), A201 (≠ L253), G202 (= G254), W204 (≠ Y256), H223 (≠ K276), Y257 (≠ A303), G331 (= G356), I332 (≠ L357), V358 (≠ L385), W359 (≠ G386), V360 (≠ W387), T361 (= T388)
Sites not aligning to the query:
1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
21% identity, 96% coverage: 2:418/433 of query aligns to 3:390/827 of 1pj7A
- active site: H222 (≠ K276), Y256 (≠ A303)
- binding flavin-adenine dinucleotide: G8 (= G7), G10 (= G9), I11 (≠ V10), V12 (≠ I11), D32 (= D30), Q33 (≠ R31), G41 (= G42), S42 (≠ N43), T43 (≠ A44), H45 (≠ Q46), P47 (= P58), L49 (≠ I60), T170 (≠ D223), V171 (≠ I224), A200 (≠ L253), G201 (= G254), W203 (≠ Y256), H222 (≠ K276), Y256 (≠ A303), I331 (≠ L357), V357 (≠ L385), W358 (≠ G386), V359 (≠ W387), T360 (= T388)
Sites not aligning to the query:
- active site: 549
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696
3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
21% identity, 96% coverage: 2:418/433 of query aligns to 3:390/827 of 3gsiA
- active site: H222 (≠ K276), Y256 (≠ A303)
- binding flavin-adenine dinucleotide: G10 (= G9), I11 (≠ V10), V12 (≠ I11), D32 (= D30), Q33 (≠ R31), G41 (= G42), S42 (≠ N43), T43 (≠ A44), H45 (≠ Q46), P47 (= P58), L49 (≠ I60), T170 (≠ D223), V171 (≠ I224), A200 (≠ L253), G201 (= G254), W203 (≠ Y256), H222 (≠ K276), Y256 (≠ A303), G330 (= G356), I331 (≠ L357), F332 (≠ R358), V357 (≠ L385), W358 (≠ G386), V359 (≠ W387), T360 (= T388)
- binding magnesium ion: D254 (≠ K301)
Sites not aligning to the query:
- active site: 549
- binding magnesium ion: 409
- binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696
Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
21% identity, 96% coverage: 2:418/433 of query aligns to 6:393/830 of Q9AGP8
- IV 14:15 (≠ VI 10:11) binding
- DQ 35:36 (≠ DR 30:31) binding
- STSH 45:48 (≠ NAGQ 43:46) binding
- L52 (≠ I60) binding
- V174 (≠ I224) binding
- H225 (≠ K276) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
- Y259 (≠ A303) Important for catalytic activity; binding ; mutation to F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
- VWVT 360:363 (≠ LGWT 385:388) binding
Sites not aligning to the query:
- 539 binding
- 552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
27% identity, 37% coverage: 245:403/433 of query aligns to 194:348/369 of S5FMM4
- S202 (≠ L253) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ W387) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ L397) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Sites not aligning to the query:
- 51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- 54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- 81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
Query Sequence
>HSERO_RS08375 FitnessBrowser__HerbieS:HSERO_RS08375
MRVVILGSGVIGVTSAWYLARAGHDVTVLDRQPGPALETSFGNAGQISPGYASPWAAPGI
PLKAIKWMFQEHAPLAIRLDGSFNQLRWMWQMLRNCNAERYAVNKERMVRLAEYSRDCLR
DLRADVGIPYEGRQQGTLQLFRSQEQLEGAAKDIAVLKEAGVPFELLTPDQLGNAEPALA
KVRDKLTGGLRLPNDETGDCQLFTTRLATMAEQIGVKFRYGVDIDALAMAGGKIAGVVCG
KELVQADSYVVALGSYSPQLLGEVPGLPAIPVYPLKGYSITVPITDASAAPVSTILDETY
KIAVTRFDDRIRVGGMAEIVGYDTALKAKRRATLELVVNDLFPGAGDTAQASFWTGLRPM
TPDGTPVVGATPVSNLYINTGHGTLGWTMSCGSGQLLADLISGRRPAIAHEDLSVERYLS
SGGRAQTPRLAGA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory