SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS12110 FitnessBrowser__HerbieS:HSERO_RS12110 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

4ru1A Crystal structure of carbohydrate transporter acei_1806 from acidothermus cellulolyticus 11b, target efi-510965, in complex with myo-inositol
30% identity, 93% coverage: 21:327/331 of query aligns to 9:283/288 of 4ru1A

query
sites
4ru1A

L

M

I

I

S

T

H
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H

A

Q

P

Q

D

P

S

G

D
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D

S

T

W

F

W
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W

N

D

T

I

I

I

K

R

N

K

A

G

I

A

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L

Q

A

A

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A

E

A

D

K

F

D

D

N

V

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Y

Y

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S

N

N

P

-

S

-

N

D

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P

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D

L

S

A

T

D

K

M

E

A

A

R

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L

V

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E

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D

A

A

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A

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A

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A

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I

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S

A

G

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T

I

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S

K

E

E

Q

S

L

-

G

G

A

A

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M

H

Y

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G

G

Q

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P

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E

E

Y

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A

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Y

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A

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E

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G

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F

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C

C

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H
x
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Y

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x
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L

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E

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R

C

C

R

N

G

G

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F

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K

A

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Y

Y

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-

G

G

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P

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L

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M

A

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S

A

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K

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N

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L

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A

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P

Y

Y

L

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E

G

G

Y

Y

I

-

P

-

V

E

A

A

I

V

L

D

A

S

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S

W

R

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Y

L

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T

N

N

G

D

D

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T

A

-

V

-

L

-

Q

-

D

-

K

-

L

-

K

-

G

-

N

-

A

-

K

-

F

-

Q

-

A

-

R

-

L

-

A

-

E

-

Y

-

G

-

L

-

A

-

P

-

I

I

Y

G

G

G

P

G

R

E

H

A

I

V

S

K

S

T

G

G

P

P

G

F

Y

F

I

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T

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R

K

D

S

N

N

L

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A

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A

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K

Y

F

A

A

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: H12 (= H24), D17 (= D29), W20 (= W32), N92 (= N106), Q135 (≠ H150), Q141 (≠ S156), R145 (= R160), L190 (= L206), D215 (= D233), Q235 (= Q253)

3c6qC Apo and ligand-bound form of a thermophilic glucose/xylose binding protein
24% identity, 89% coverage: 31:325/331 of query aligns to 14:294/305 of 3c6qC

query
sites
3c6qC

W

Y

W
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W

N

S

T

Q

I

V

K

E

N

Q

A

G

I

V

K

K

Q

A

A

A

G

G

E

K

D

A

F

L

D

G

V

V

S

D

V

T

D

K

Y

F

R

F

N

V

P

P

S

Q

N

K

G

C

D

D

L

I

A

N

D

A

M

Q

A

L

R

Q

L

M

V

L

E

E

Q

S

A

F

A

I

A

A

R

E

N

G

Y

V

N

N

G

G

V

I

V

A

V

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S

A

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A

S

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D

F

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K

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P

T

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E

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A

E

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M

N

G

I

I

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P

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V

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V

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T

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x
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S

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G

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T

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Q

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G

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N

Y

Y

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A

A

G

G

Y

Y

G

T

A

A

G

G

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E

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L

A

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G

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G

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G

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F

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A

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T

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L

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N

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C

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x
E

A

D

V

G

E

A

S

R

K

A

V

V

S

S

-

L

-

A

-

E

A

A

Y

A

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L

R

N

N

A

N

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P

G

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T

L

Q

D

A

A

V

F

L

F

A

G

L

V

-
x
Y

-
x
A

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Y

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N

G

G

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P

D

A

S

Q

A

A

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L

P

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S

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L

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R

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A

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K

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M

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G

L

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K

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I

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C

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F

D
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L

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T

E

P

E

D

I

I

A

L

N

Q

G

Y

I

V

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K

D

E

G

G

S

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V

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F

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Q

R

P

P

Y

Y

L

M

Q

M

G

G

Y

Y

I

L

P

S

V

V

A

T

I

V

L

L

A

Y

L

L

S

M

R

N

Q

K

L

I

K

G

T

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Q

D

N

V

T

A

L

V

M

L

M

Q

L

D

P

K

K

L

V

K

K

G

V

N

D

A

G

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K

F

V

Q

D

A

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R

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L

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A

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E

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Y

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G

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L

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A

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P

-

I

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Y

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G

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P

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R

Y

H

V

I

I

S

D

S

T

G

G

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E

N

N

L

L

D

D

K

E

V

Y

L

L

K

K

binding beta-D-xylopyranose: W15 (= W32), D89 (≠ N106), N137 (≠ S156), R141 (= R160), E164 (≠ N184), Y190 (vs. gap), A191 (vs. gap), D216 (= D233), Q236 (= Q253)

Sites not aligning to the query:

binding beta-D-xylopyranose: 9

2h3hA Crystal structure of the liganded form of thermotoga maritima glucose binding protein (see paper)
24% identity, 89% coverage: 31:325/331 of query aligns to 14:294/313 of 2h3hA

query
sites
2h3hA

W

Y

W

W

N

S

T

Q

I

V

K

E

N

Q

A

G

I

V

K

K

Q

A

A

A

G

G

E

K

D

A

F

L

D

G

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V

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D

V

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D

K

Y

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N

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N

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G

E

D

D

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A

N

D

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M

Q

A

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Q

L

M

V

L

E

E

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S

A

F

A

I

A

A

R

E

N

G

Y

V

N

N

G

G

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I

V

A

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S

A

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A

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D

F

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A

L

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K

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P

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I

I

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A

V

L

A

E

K

M

N

G

I

I

P

P

V

V

I

V

T

T

I

L

N
x
D

S

T

G

-

T

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Q

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Q

D

S

S

E

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Q

D

L

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G

G

A

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L

Y

M

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H

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G

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D

E

N

Y

Y

D

Q

A

A

G

G

Y

Y

G

T

A

A

G

G

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L

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Q

M

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K

A

E

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A

L

A

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G

G

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C

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G

Y

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A

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A

P

M

S
x
N

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S

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E

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R
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R

C

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Q

G

G

F

F

A

K

E

D

A

A

I

I

G

-

A

K

D

D

Y

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K

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K

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S

E

T

I

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V

D

D

T

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G

L

I

N

D

D

P

E

N
x
E

A

D

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G

E

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K

A

V

V

S

S

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L

-

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E

A

A

Y

A

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N

N

A

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D

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D

A

A

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F

L

F

A

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L

V

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x
Y

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x
A

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Y

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N

G

G

P

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D

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A

A

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L

P

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Q

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P

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G

Y

Y

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A

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Y

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M

L

M

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L

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P

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K

L

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V

N

D

A

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F

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Q

D

A

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R

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A

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Y

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I

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Y

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R

Y

H

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I

S

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S

T

G

G

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G

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T

T

R

P

D

E

N

N

L

L

D

D

K

E

V

Y

L

L

K

K

binding beta-D-glucopyranose: D89 (≠ N106), N137 (≠ S156), R141 (= R160), E164 (≠ N184), Y190 (vs. gap), A191 (vs. gap), D216 (= D233), Q236 (= Q253)

Sites not aligning to the query:

binding beta-D-glucopyranose: 9

6gt9A Crystal structure of ganp, a glucose-galactose binding protein from geobacillus stearothermophilus, in complex with galactose
24% identity, 75% coverage: 19:267/331 of query aligns to 9:257/283 of 6gt9A

query
sites
6gt9A

F

F

V

V

L

L

I

V

S

P

H
x
E

A

E

P

L

D

D

S
x
N

D

D

S

-

W
x
Y

W
|
W

N

R

T

L

I

V

K

E

N

K

A

G

I

A

K

K

Q

A

A

A

G

A

E

K

D

E

F

L

D

G

V

V

S

D

V

L

D

E

Y

Y

R

I

N

G

P

P

S

R

N

Q

G

A

D

N

L

I

A

D

D

E

M

H

A

L

R

R

L

I

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L

E

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Q

K

A

A

R

A

N

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Y

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N

D

G

G

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S

Q

I

G

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E

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F

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K

P

P

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M

E

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T

A

D

K

K

N

N

I

I

P

P

V

V

I

V

T

T

I

I

N
x
D

S

T

G

D

T

A

Q

P

Q

T

Q

S

S

R

E

R

Q

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L

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G

-

A

-

L

V

M

A

H

Y

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V

G

G

Q

T

P

D

E

N

Y

Y

D

Y

A

A

G

G

Y

F

G

L

A

A

G

G

Q

R

-

A

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L

-

A

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E

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D

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T

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K

-

G

K

K

A

A

K

T

A

V

A

A

G

I

I

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T

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G

F

S

L

L

C

T

V

A

N

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H
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H

Y

Q

A

Q

T

L

N

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P

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S

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F

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E

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R
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R

C

V

R

R

G

G

F

F

A

E

E

D

A

A

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G

R

A

Q

D

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Y

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K

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T

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G

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I

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A

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I

E

E

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E

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S

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H

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x
I

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T

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R

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V

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Q

-

A

S

E

K

K

V

A

S

Y

A

T

Y

I

L

L

R

K

N

K

N

H

P

P

G

D

T

V

Q

N

A

A

V

F

L

Y

A

G

L

T

G
x
S

P
x
A

D

L

S

D

A

A

V

I

P

G

S

V

L

A

R

K

A

V

L

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Q

E

K

Q

M

F

G

H

L

R

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E

S

Q

K

K

I

T

W

Y

F

I

A

I

T

G

F

F

D
|
D

L

T

S

L

E

P

E

E

I

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A

I

N

R

G

Y

I

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D

K

G

G

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T

V

I

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A

F

A

A

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I

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D

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Q
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Q

Q

E

P

P

Y

Y

L

E

Q

M

G

G

Y

Y

I

K

P

A

V

V

A

K

I

M

L

M

A

A

binding beta-D-galactopyranose: E14 (≠ H24), N18 (≠ S28), Y20 (≠ W31), W21 (= W32), D95 (≠ N106), H143 (= H150), R147 (= R160), I171 (vs. gap), S197 (≠ G207), A198 (≠ P208), D223 (= D233), Q243 (= Q253)

6guqA Crystal structure of ganp, a glucose-galactose binding protein from geobacillus stearothermophilus, in complex with glucose
24% identity, 76% coverage: 18:267/331 of query aligns to 3:252/278 of 6guqA

query
sites
6guqA

R

H

F

F

V

V

L

L

I

V

S

P

H
x
E

A

E

P

L

D

D

S
x
N

D

D

S

-

W
x
Y

W

W

N

R

T

L

I

V

K

E

N

K

A

G

I

A

K

K

Q

A

A

A

G

A

E

K

D

E

F

L

D

G

V

V

S

D

V

L

D

E

Y

Y

R

I

N

G

P

P

S

R

N

Q

G

A

D

N

L

I

A

D

D

E

M

H

A

L

R

R

L

I

V

L

E

K

Q

K

A

A

R

A

N

K

Y

V

N

D

G

G

V

I

V

T

S

Q

I

G

A

L

D

T

F

E

S

A

V

E

L

F

K

V

K

P

P

V

I

I

E

N

M

E

V

I

V

T

A

D

K

K

N

N

I

I

P

P

V

V

I

V

T

T

I

I

N
x
D

S

T

G

D

T

A

Q

P

Q

T

Q

S

S

R

E

R

Q

-

L

-

G

-

A

-

L

V

M

A

H

Y

V

V

G

G

Q

T

P

D

E

N

Y

Y

D

Y

A

A

G

G

Y

F

G

L

A

A

G

G

Q

R

-

A

-

L

-

A

-

E

-

D

-

T

-

K

-

G

K

K

A

A

K

T

A

V

A

A

G

I

I

I

K

T

S

G

F

S

L

L

C

T

V

A

N

A

H
|
H

Y

Q

A

Q

T

L

N

-

P

-

S

-

F

-

E

-

R
|
R

C

V

R

R

G

G

F

F

A

E

E

D

A

A

I

V

G

R

A

Q

D

-

Y

-

K

-

S

-

T

-

L

-

D

E

T

K

G

G

I

I

D

R

P

I

N

V

A

A

V

I

E

E

-

E

-

S

-

H

-
x
I

-

T

-

R

-

V

-

Q

-

A

S

E

K

K

V

A

S

Y

A

T

Y

I

L

L

R

K

N

K

N

H

P

P

G

D

T

V

Q

N

A

A

V

F

L

Y

A

G

L

T

G
x
S

P
x
A

D

L

S

D

A

A

V

I

P

G

S

V

L

A

R

K

A

V

L

V

Q

E

K

Q

M

F

G

H

L

R

K

E

S

Q

K

K

I

T

W

Y

F

I

A

I

T

G

F

F

D
|
D

L

T

S

L

E

P

E

E

I

T

A

I

N

R

G

Y

I

L

K

Q

D

K

G

G

S

T

V

I

Q

A

F

A

A

T

I

V

D

V

Q
|
Q

Q

E

P

P

Y

Y

L

E

Q

M

G

G

Y

Y

I

K

P

A

V

V

A

K

I

M

L

M

A

A

binding beta-D-glucopyranose: E9 (≠ H24), N13 (≠ S28), Y15 (≠ W31), D90 (≠ N106), H138 (= H150), R142 (= R160), I166 (vs. gap), S192 (≠ G207), A193 (≠ P208), D218 (= D233), Q238 (= Q253)

5xssA Xylfii molecule (see paper)
24% identity, 75% coverage: 18:266/331 of query aligns to 5:253/274 of 5xssA

query
sites
5xssA

R

K

F

I

V

V

L

L

I

I

S

S

H
|
H

A

I

P

K

D

-

S

T

D

N

S

P

W

Y

W
|
W

N

L

T

D

I

I

K

K

N

A

A

G

I

A

K

E

Q

R

A

A

G

A

E

K

D

E

F

R

D

G

V

A

S

V

V

V

D

E

Y

F

R

L

N

G

P

P

S

T

N

T

G

A

D

S

L

T

A

E

D

D

M

G

A

L

R

K

L

L

V

F

E

D

Q

M

A

A

A

T

A

S

R

A

N

K

Y

V

N

S

G

G

V

I

V

T

S

Y

I

V

A

Q

D

E

F

E

S

G

V

Q

L

Y

K

K

P

K

I

I

E

N

M

S

V

A

V

M

A

E

K

K

N

G

I

I

P

P

V

V

I

V

T

T

I

I

N
x
D

S

S

G

-

T

-

Q

-

Q

D

S

E

E

E

Q

D

L

S

G

N

A

R

L

I

M

A

H

Y

V

V

G

G

Q

T

P

D

E

N

Y

V

D

L

A

A

G

G

Y

Q

G

V

A

A

G

G

Q

K

K

E

-

M

A

V

K

K

A

Q

A

I

G

G

I

T

K

S

S

G

F

N

L

V

C

A

V

I

N

V

H

M

Y

G

A

G

T

K

N

N

-

V

P

K

S

N

S

Q

F

K

E

E

R
|
R

C

V

R

E

G

G

F

F

A

T

E

Q

A

Y

I

I

G

K

A

S

D

N

Y

S

K

N

K

L

S

K

T

I

L

V

D

D

T

T

-

D

G

S

I

S

D

D

P

A

N

M

A

L

V

L

E

E

S

A

K

E

V

I

-

I

-

T

S

R

A

K

Y

I

L

L

R

N

N

R

N

N

P

D

G

N

T

I

Q

N

A

A

V

L

L

F

A

C

L

T

G
x
S

P
x
A

D

L

S

D

A

G

V

I

P

G

S

A

L

A

R

R

A

A

L

V

Q

K

K

D

M

L

G

N

L

Y

K

K

S

D

K

R

I

V

W

K

F

I

A

I

T

C

F

F

D
|
D

L

D

S

L

E

D

I

T

A

L

N

S

G

N

I

I

K

R

D

N

G

G

S

L

V

V

Q

S

F

A

A

T

I

I

D

V

Q
|
Q

Q

K

P

S

Y

N

L

E

Q

M

G

G

Y

Y

I

R

P

A

V

V

A

N

I

I

L

I

binding beta-D-xylopyranose: H11 (= H24), W18 (= W32), D92 (≠ N106), R144 (= R160), S194 (≠ G207), A195 (≠ P208), D220 (= D233), Q240 (= Q253)

4rxmB Crystal structure of periplasmic abc transporter solute binding protein a7jw62 from mannheimia haemolytica phl213, target efi-511105, in complex with myo-inositol
26% identity, 57% coverage: 67:255/331 of query aligns to 51:243/288 of 4rxmB

query
sites
4rxmB

V

L

E

E

Q

N

A

A

A

I

A

T

R

R

N

G

Y

A

N

K

G

G

V

I

S

S

I

P

A

N

D

D

F

V

S

N

V

A

L

I

K

S

K

G

P

A

I

V

E

E

M

E

V

I

A

K

K

E

N

K

I

I

P

P

V

A

I

A

T

T

I

L

N
x
D

S

-

G

-

T

-

Q

-

Q
x
R

Q

K

S

V

E

E

Q

S

L

S

G

K

A

P

L

V

M

P

H

H

V

F

G

G

Q

A

P

N

E

N

Y

Y

D

T

A

G

G

G

Y

Q

G

E

A

V

G

A

Q

K

K

A

A

V

K

K

A

A

A

K

-

Y

-

P

-

N

G

G

I

A

K

K

S

I

F

I

L

L

C

L

V

T

N

G

H

Q

Y

P

A

G

T

S

N

T

P

-

S
|
S

S

N

F

I

E

E

R
|
R

C

T

R

K

G

G

F

I

A

R

E

D

-

E

-

L

A

A

I

A

G

G

A

G

D

D

Y

K

K

Y

K

K

S

I

T

V

L

V

D

D

-

Q

T

T

G

G

I

-

D

-

P

-

N

N

A
x
W

V

L

E

R

S

S

K

E

-

G

-

L

-

R

-

I

-

E

-

S

V

V

S

L

A

P

Y

T

L
|
L

R
x
K

N
x
E

N

K

P

P

G

-

T

-

Q

E

A

V

V

I

L

I

A

S

L

A

G
x
N

P

D

D

D

S

M

A

A

V

L

P

G

S

A

L

I

R

E

A

A

L

L

Q

R

K

S

M

Q

G

G

L

L

K

K

S

A

-

G

K

D

I

I

W

L

F

V

A

T

T

G

F

F

D
|
D

L

S

S

T

E

P

E

E

I

A

A

L

N

A

G

R

I

V

K

K

D

D

G

G

S

W

V

L

Q

Y

F

L

A

T

I

A

D

D

Q
|
Q

Q

R

P

P

binding aspartic acid: L183 (= L194), K184 (≠ R195), E185 (≠ N196)
binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: D90 (≠ N106), R91 (≠ Q111), S138 (= S156), R142 (= R160), W167 (≠ A185), N194 (≠ G207), D221 (= D233), Q241 (= Q253)

Sites not aligning to the query:

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: 17

4rxmA Crystal structure of periplasmic abc transporter solute binding protein a7jw62 from mannheimia haemolytica phl213, target efi-511105, in complex with myo-inositol
26% identity, 57% coverage: 67:255/331 of query aligns to 53:245/291 of 4rxmA

query
sites
4rxmA

V

L

E

E

Q

N

A

A

A

I

A

T

R

R

N

G

Y

A

N

K

G

G

V

I

S

S

I

P

A

N

D

D

F

V

S

N

V

A

L

I

K

S

K

G

P

A

I

V

E

E

M

E

V

I

A

K

K

E

N

K

I

I

P

P

V

A

I

A

T

T

I

L

N
x
D

S

-

G

-

T

-

Q

-

Q
x
R

Q

K

S

V

E

E

Q

S

L

S

G

K

A

P

L

V

M

P

H

H

V

F

G

G

Q

A

P

N

E

N

Y

Y

D

T

A

G

G

G

Y

Q

G

E

A

V

G

A

Q

K

K

A

A

V

K

K

A

A

A

K

-

Y

-

P

-

N

G

G

I

A

K

K

S

I

F

I

L

L

C

L

V

T

N

G

H

Q

Y

P

A

G

T

S

N

T

P

-

S
|
S

S

N

F

I

E

E

R
|
R

C

T

R

K

G

G

F

I

A

R

E

D

-

E

-

L

A

A

I

A

G

G

A

G

D

D

Y

K

K

Y

K

K

S

I

T

V

L

V

D

D

-

Q

T

T

G

G

I

-

D

-

P

-

N

N

A
x
W

V

L

E

R

S

S

K

E

-

G

-

L

-

R

-

I

-

E

-

S

V

V

S

L

A

P

Y

T

L

L

R

K

N

E

N

K

P

P

G

-

T

-

Q

E

A

V

V

I

L

I

A

S

L

A

G
x
N

P

D

D

D

S

M

A

A

V

L

P

G

S

A

L

I

R

E

A

A

L

L

Q

R

K

S

M

Q

G

G

L

L

K

K

S

A

-

G

K

D

I

I

W

L

F

V

A

T

T

G

F

F

D
|
D

L

S

S

T

E

P

E

E

I

A

A

L

N

A

G

R

I

V

K

K

D

D

G

G

S

W

V

L

Q

Y

F

L

A

T

I

A

D

D

Q
|
Q

Q

R

P

P

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: D92 (≠ N106), R93 (≠ Q111), S140 (= S156), R144 (= R160), W169 (≠ A185), N196 (≠ G207), D223 (= D233), Q243 (= Q253)

Sites not aligning to the query:

2ioyA Crystal structure of thermoanaerobacter tengcongensis ribose binding protein (see paper)
24% identity, 71% coverage: 31:265/331 of query aligns to 14:247/274 of 2ioyA

query
sites
2ioyA

W
x
F

N

V

T

T

I

L

K

K

N

N

A

G

I

A

K

E

Q

E

A

K

G

A

E

K

D

E

F

L

D

G

V

Y

S

K

V

I

-

I

-

V

-

E

D

D

Y

S

R

Q

N

N

P

D

S

S

N

S

G

K

D

E

L

L

A

S

D

N

M

V

A

E

R

D

L

L

V

I

E

Q

Q

Q

A

-

R

-

N

K

Y

V

N

D

G

V

V

L

V

I

S

N

I

P

A

V

D

D

F

S

S

D

V

A

L

V

K

V

K

T

P

A

I

I

E

K

M

E

V

A

V

N

A

S

K

K

N

N

I

I

P

P

V

V

I

I

T

T

I

I

N
x
D

S
x
R

G

S

T

A

Q

N

Q

G

S

D

E

-

Q

-

L

-

G

-

A

V

L

V

M

C

H

H

V

I

G

A

Q

S

P

D

E

N

Y

V

D

K

A

G

G

G

Y

E

G

M

A

A

G

A

Q

E

-

F

K

I

A

A

K

K

A

A

-

L

A

K

G

G

I

K

K

G

S

N

F

V

L

V

C

E

V

L

N

E

H

G

Y

I

A

P

T

G

N

A

P

S

S
x
A

S

A

F

R

E

D

R
|
R

C

G

R

K

G

G

F

F

A

D

E

E

A

A

I

I

G

A

A

K

-

Y

-

P

D

D

Y

I

K

K

-

I

-

V

-

A

K

K

S

Q

T

A

L

A

D

D

T

-

G

-

I
x
F

D

D

P

R

N

S

A

K

V

G

E

L

S

S

K

V

V

M

S

E

A

N

Y

I

L

L

R

Q

N

A

N

Q

P

P

G

K

T

I

Q

D

A

A

V

V

L

F

A

A

L

Q

G
x
N

P

D

D

E

S

M

A

A

V

L

P

G

S

A

L

I

R

K

A

A

L

I

Q

E

K

A

M

A

G

N

L

-

K

R

S

Q

K

G

I

I

W

I

F

V

A

V

T

G

F

F

D
|
D

L

G

S

T

E

E

E

D

I

A

A

L

N

K

G

A

I

I

K

K

D

E

G

G

S

K

V

M

Q

A

F

A

A

T

I

I

D

A

Q
|
Q

Q

Q

P

P

Y

A

L

L

Q

M

G

G

Y

S

I

L

P

G

V

V

A

E

I

M

binding beta-D-ribopyranose: F14 (≠ W31), F15 (≠ W32), D88 (≠ N106), R89 (≠ S107), A136 (≠ S156), R140 (= R160), F164 (≠ I181), N190 (≠ G207), D215 (= D233), Q235 (= Q253)

Sites not aligning to the query:

binding beta-D-ribopyranose: 12

1dbpA Identical mutations at corresponding positions in two homologous proteins with non-identical effects (see paper)
26% identity, 63% coverage: 48:255/331 of query aligns to 35:237/271 of 1dbpA

query
sites
1dbpA

V

V

S

V

V

L

D

D

Y

S

R

Q

N

N

P

N

S

P

N

A

G

K

D

E

L

L

A

A

D

N

M

-

A

-

R

-

L

-

V

V

E

Q

Q

D

A

L

A

T

A

V

R

R

N

G

Y

T

N

K

G

I

V

L

V

I

S

N

I

P

A

T

D

D

F

S

S

D

V

A

L

V

K

D

K

N

P

A

I

V

E

K

M

M

V

A

V

N

A

Q

K

A

N

N

I

I

P

P

V

V

I

I

T

T

I

L

N
x
D

S

-

G

-

T

-

Q

-

Q
x
R

Q

Q

S

A

E

T

Q

K

L

G

G

E

A

V

L

V

M

S

H

H

V

I

G

A

Q

S

P

D

E

N

Y

V

D

L

A

G

G

G

Y

K

G

I

A

A

G

G

Q

D

K

Y

-

I

A

A

K

K

A

K

A

A

G

G

I

E

K

G

S

A

-

K

F

V

L

I

C

E

V

L

N

Q

H

G

Y

I

A

A

T

G

N

T

P

S

S

A

F

R

E

E

R
|
R

C

G

R

E

G

G

F

F

A

Q

E

Q

A

A

I

V

G

A

A

A

D

-

Y

H

K

K

K

F

S

N

T

V

L

L

-

A

-

S

-

Q

-

P

-

A

-

D

-
x
F

D

D

T

R

G

I

I

K

D

G

P

L

N

N

A

V

V

M

E

Q

S

N

K

L

V

L

S

T

A

A

Y

H

L

-

R

-

N

-

P

P

G

D

T

V

Q

Q

A

A

V

V

L

F

A

A

L

Q

G
x
N

P

D

D

E

S

M

A

A

V

L

P

G

S

A

L

L

R

R

A

A

L

L

Q

Q

K

T

M

A

G

G

L

-

K

K

S

S

K

D

I

V

W

M

F

V

A

V

T

G

F

F

D
|
D

L

G

S

T

E

P

E

D

I

G

A

E

N

K

G

A

I

V

K

N

D

D

G

G

S

K

V

L

Q

A

F

A

A

T

I

I

D

A

Q

Q

Q

L

P

P

binding beta-D-ribopyranose: D89 (≠ N106), R90 (≠ Q111), R141 (= R160), F164 (vs. gap), N190 (≠ G207), D215 (= D233)

Sites not aligning to the query:

binding beta-D-ribopyranose: 13, 15

4wutA Crystal structure of an abc transporter solute binding protein (ipr025997) from agrobacterium vitis (avi_5133, target efi-511220) with bound d-fucose
20% identity, 73% coverage: 18:259/331 of query aligns to 2:248/290 of 4wutA

query
sites
4wutA

R

E

F

I

V

A

L

V

I

I

S

V

H
x
K

A

T

P

V

D

N

S

S

D

T

S

F

W
|
W

W

Q

N

N

T

V

I

Q

K

K

N

G

A

A

I

D

K

A

Q

A

A

I

G

G

E

K

D

Q

F

K

D

A

V

H

S

T

V

I

D

T

Y

F

R

Q

N

G

P

P

S

A

-

A

N
x
E

G

S

D

A

L

I

A

A

D

D

M

Q

A

V

R

N

L

M

V

V

E

E

Q

N

A

A

A

V

A

N

R

R

N

K

Y

V

N

D

G

A

V

I

V

L

S

A

I

P

A

S

D

D

F

P

S

D

V

A

L

L

K

V

K

P

P

A

I

V

E

K

M

K

V

A

V

W

A

E

K

A

N

R

I

I

P

P

V

V

I

V

T

I

I

I

N
x
D

S

S

G

M

T

L

Q

S

Q

K

Q

D

S

A

E

E

Q

K

L

Y

G

Y

A

Q

L

A

M

F

H

L

V

A

G

T

Q

D

P

N

E

K

Y

A

D

A

A

G

G

E

Y

L

G

A

A

A

G

K

Q

A

K

M

A

I

K

Q

A

K

A

V

G

G

I

T

K

E

S

G

F

K

L

I

C

A

V

V

N

M

H

S

Y

Y

A

V

T

A

N

G

P

A

-

G

S

S

S

E

F

I

E

G

R
|
R

C

V

R

G

G

G

F

F

A

T

E

D

A

Y

I

I

G
x
K

A

A

D

N

-
x
S

-

K

-

L

-

Q

-

I

-

V

-

G

-

P

-

Y

Y

Y

K

S

K

Q

S

S

T

Q

L

M

D

A

T

T

G

A

I

L

D
x
N

P

-

N

-

A

-

V

-

E

-

S

-

K

Q

V

T

S

T

A
x
D

Y

V

L

L

R

A

N

A

N

N

P

A

G

D

T

L

Q

K

A

G

V

I

L

F

A

G

L

A

G
x
N

P

E

D

P

S

T

A

A

V

I

P

G

S

M

L

G

R

R

A

A

L

I

Q

K

K

Q

M

A

G

G

L

K

K

A

S

G

K

K

I

L

W

V

F

A

A

I

T

G

F

F

D
|
D

L

G

S

N

E

Q

E

D

I

L

A

Q

N

E

G

F

I

V

K

K

D

D

G

G

S

T

V

L

Q

E

F

A

A

T

I

V

D

V

Q
|
Q

Q

G

P

S

Y

Y

L

Q

Q

M

G

G

binding calcium ion: K155 (≠ G169), S158 (vs. gap), N177 (≠ D182), D181 (≠ A192)
binding beta-D-fucopyranose: K8 (≠ H24), W15 (= W31), E42 (≠ N57), D91 (≠ N106), R146 (= R160), N196 (≠ G207), D222 (= D233), Q242 (= Q253)

3ksmA Crystal structure of abc-type sugar transport system, periplasmic component from hahella chejuensis
19% identity, 72% coverage: 27:263/331 of query aligns to 10:251/276 of 3ksmA

query
sites
3ksmA

D

D

S

S

D

N

S

A

W
x
Y

W

W

N

R

T

Q

I

V

K

Y

N

L

A

G

I

A

K

Q

Q

K

A

A

G

A

E

D

D

E

F

A

D

G

V

V

S

T

V

L

D

L

Y

H

R

R

N

S

P

T

S

K

N

D

-

D

G

G

D

D

L

I

A

A

D

G

M

Q

A

I

R

Q

L

I

V

L

E

S

Q

Y

A

H

A

L

A

S

R

Q

N

A

Y

P

-

P

N

D

G

A

V

L

V

I

V

L

S

A

I

P

A

N

D

S

F

A

S

E

V

D

L

L

K

T

K

P

P

S

I

V

E

A

M

Q

V

Y

V

R

A

A

K

R

N

N

I

I

P

P

V

V

I

L

T

V

I

V

N
x
D

S

S

G

D

T

L

Q

A

Q

G

Q

D

S

A

E

H

Q

Q

L

G

G

L

A

V

L

A

M

T

H

D

V

N

G

Y

Q

A

P

A

E

G

Y

Q

D

L

A

A

G

A

Y

R

G

A

A

L

G

L

Q

A

K

T

A

L

K

D

A

L

A

S

G

K

I

E

K

R

S

N

F

I

L

A

C

L

V

L

N

R

H
x
L

Y

R

A

A

T

G

N
|
N

P

A

S
|
S

S

T

F

D

E

Q

R
|
R

C

E

R

Q

G

G

F

F

A

L

E

D

A

V

I

L

G

R

A

K

D

H

Y

D

K

K

K

I

S

R

T

I

L

I

D

A

T

A

-

P

-

Y

-

A

G

G

I

D

D

D

P

R

N

G

A

A

V

A

E

R

S

S

K

E

V

M

S

L

A

R

Y

L

L

L

R

K

N

E

N

T

P

P

G

T

T

I

Q

D

A

G

V

L

L

F

A

T

L

P

G
x
N

P

E

D

S

S

T

A

T

V

I

P

G

S

A

L

L

R

V

A

A

L

I

Q

R

K

Q

M

S

G

G

L

M

K

S

S

K

K

Q

I

F

W

G

F

F

A

I

T

G

F

F

D
|
D

L

Q

S

T

E

E

I

L

A

E

N

A

G

A

I

M

K

Y

D

A

G

G

S

E

V

I

Q

S

F

N

A

L

I

V

D

V

Q
|
Q

Q

N

P

P

Y

E

L

Y

Q

M

G

G

Y

Y

I

L

P

A

V

V

binding beta-D-ribofuranose: Y14 (≠ W31), D91 (≠ N106), L135 (≠ H150), N139 (= N154), S141 (= S156), R145 (= R160), N195 (≠ G207), D221 (= D233), Q241 (= Q253)

Sites not aligning to the query:

binding beta-D-ribofuranose: 8

5dkvA Crystal structure of an abc transporter solute binding protein from agrobacterium vitis(avis_5339, target efi-511225) bound with alpha-d- tagatopyranose
23% identity, 59% coverage: 66:260/331 of query aligns to 55:260/303 of 5dkvA

query
sites
5dkvA

L

V

V

L

E

D

Q

A

A

V

A

I

A

A

R

K

N

K

Y

P

N

D

G

A

V

I

V

L

V

I

S

A

I

P

A

T

D

D

F

T

S

T

V

Q

L

L

K

V

K

Q

P

P

I

L

E

K

M

K

V

A

A

D

K

A

N

G

I

I

P

P

V

M

I

I

T

T

I

V

N
x
D

S

T

G

F

T

I

Q

G

Q

T

Q

G

S

D

E

Y

Q

Q

L

T

G

G

A

A

-

G

-

D

-

G

-

D

-

F

-

P

L

L

M

S

H

Y

V

I

G

A

Q

S

P

D

E

N

Y

V

D

L

A

G

G

G

Y

E

G

I

A

A

G

A

Q

R

K

S

-

L

A

A

K

L

A

A

A

I

G

G

I

D

K

K

S

G

F

K

L

V

C

Y

V

V

N

S

H

N

Y

V

A

K

T

P

N

G

P

V

S

S

S
x
T

F

T

-

D

E

Q

R
|
R

C

E

R

Q

G

G

F

F

A

K

E

S

A

E

I

M

G

A

A

K

D

H

Y

P

K

G

K

I

S

T

T

V

L

L

D

E

T

T

G

Q

I

F

-

N

-

D

-

N

D

D

P

A

N

N

A

K

V

A

E

A

S

S

K

Q

V

L

S

Q

A

A

Y

V

L

Y

R

A

N

R

N

N

P

P

G

D

T

L

Q

A

A

G

V

V

L

F

A

G

L

A

G
x
N

P
x
L

D

F

S

S

A

G

V

L

P

G

S

S

L

A

R

N

A

G

L

V

Q

Q

K

Q

M

A

G

G

L

Q

K

S

S

G

K

T

I

I

W

K

F

V

A

V

T

A

F

F

D
|
D

L

A

S

P

E

G

E

S

I

V

A

V

N

D

G

N

I

L

K

K

D

S

G

G

S

L

V

I

Q

D

F

F

A

A

I

I

D

A

Q
|
Q

Q

H

P

P

Y

A

L

E

Q

I

G

G

Y

Y

binding alpha-D-tagatopyranose: D95 (≠ N106), T153 (≠ S157), R157 (= R160), N207 (≠ G207), L208 (≠ P208), D233 (= D233), Q253 (= Q253)

Sites not aligning to the query:

binding alpha-D-tagatopyranose: 18, 20

4rxtA Crystal structure of carbohydrate transporter solute binding protein arad_9553 from agrobacterium radiobacter, target efi-511541, in complex with d-arabinose
24% identity, 69% coverage: 31:260/331 of query aligns to 18:254/295 of 4rxtA

query
sites
4rxtA

W
x
Y

W

W

N

Q

T

I

I

V

K

L

N

A

A

G

I

A

K

R

Q

K

A

A

G

G

E

K

D

D

F

L

D

G

V

V

S

K

V

V

-

P

D

E

Y

L

R

G

N

A

P

Q

S

A

N
x
E

G

T

D

D

L

V

A

N

D

G

M

Q

A

I

R

S

L

I

V

L

E

E

Q

N

A

A

A

V

A

A

R

G

N

K

Y

P

N

A

G

A

V

I

V

V

V

I

S

S

I

P

A

T

D

E

F

F

S

K

V

A

L

L

K

G

K

K

P

P

I

V

E

D

M

E

V

A

V

-

A

A

K

K

N

S

I

V

P

P

V

I

I

I

T

G

I

I

N
x
D

S

S

G

G

T

A

Q

D

Q

-

S

S

E

K

Q

A

L

F

G

K

A

S

L

F

M

L

H

T

V

T

-

D

-

N

-

T

-

Q

-

G

G

G

Q

R

P

I

E

A

Y

A

D

D

A

-

G

G

Y

L

G

A

A

A

G

A

Q

I

K

K

A

A

K

M

A

T

A

G

G

K

I

E

K

E

S

G

F

D

L

V

C

V

V

I

N

I

H

T

Y

N

A

T

T

P

N

G

P

A

S

G

S
|
S

F

L

E

E

R

Q

C
x
R

R

R

-

T

G

G

F

F

A

L

E

D

A

Q

I

V

G

K

A

T

D

K

Y

Y

K

P

-

G

-

L

K

K

S

V

T

V

L

A

D

D

T

K

G

Y

I

A

D

D

P

G

N

Q

A

A

V

T

E

T

-

G

-

L

S

N

K

I

V

M

S

T

A

D

Y

L

L

I

R

T

N

A

N

N

P

P

G

K

T

I

Q

V

A

G

V

V

L

F

A

A

L

S

G
x
N

P

L

D

I

S

M

A

A

V

Q

P

G

S

V

L

G

R

Q

A

A

L

I

Q

A

K

E

M

N

G

K

L

L

K

S

S

D

K

K

I

V

W

K

F

V

A

I

T

G

F

F

D
|
D

L

S

S

D

E

D

E

K

I

T

A

L

N

G

G

F

I

L

K

K

D

S

G

G

S

A

V

I

Q

A

F

G

A

L

I

V

D

V

Q
|
Q

Q

D

P

P

Y

Y

L

R

Q

M

G

G

Y

Y

binding alpha-L-arabinopyranose: Y18 (≠ W31), E45 (≠ N57), D93 (≠ N106), S146 (= S157), R150 (≠ C161), N201 (≠ G207), D227 (= D233), Q247 (= Q253)

Sites not aligning to the query:

binding alpha-L-arabinopyranose: 12

Query Sequence

>HSERO_RS12110 FitnessBrowser__HerbieS:HSERO_RS12110
MALLAAATPSPSVAANERFVLISHAPDSDSWWNTIKNAIKQAGEDFDVSVDYRNPSNGDL
ADMARLVEQAAARNYNGVVVSIADFSVLKKPIEMVVAKNIPVITINSGTQQQSEQLGALM
HVGQPEYDAGYGAGQKAKAAGIKSFLCVNHYATNPSSFERCRGFAEAIGADYKKSTLDTG
IDPNAVESKVSAYLRNNPGTQAVLALGPDSAVPSLRALQKMGLKSKIWFATFDLSEEIAN
GIKDGSVQFAIDQQPYLQGYIPVAILALSRQLKTNDVAVLQDKLKGNAKFQARLAEYGLA
PIYGPRHISSGPGYITRDNLDKVLKYAGQYR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory