SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS17000 FitnessBrowser__HerbieS:HSERO_RS17000 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5iaiA Crystal structure of abc transporter solute binding protein arad_9887 from agrobacterium radiobacter k84, target efi-510945 in complex with ribitol
31% identity, 87% coverage: 43:410/423 of query aligns to 20:391/399 of 5iaiA

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binding D-ribitol: D67 (= D90), Q176 (≠ W195), W227 (= W245), W245 (≠ S263), G283 (= G297), W284 (≠ H298), R357 (≠ K372)

Sites not aligning to the query:

binding D-ribitol: 15

4ryaA Crystal structure of abc transporter solute binding protein avi_3567 from agrobacterium vitis s4, target efi-510645, with bound d-mannitol
34% identity, 67% coverage: 44:327/423 of query aligns to 17:306/417 of 4ryaA

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4ryaA

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binding d-mannitol: E40 (= E66), R44 (= R70), Y114 (≠ I139), E116 (≠ G141), R163 (= R185), G169 (= G191), F224 (≠ W245), W279 (≠ Y296), W281 (≠ H298)

Sites not aligning to the query:

binding d-mannitol: 367

6dtqA Maltose bound t. Maritima male3 (see paper)
32% identity, 56% coverage: 95:329/423 of query aligns to 72:307/391 of 6dtqA

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6dtqA

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binding alpha-D-glucopyranose: D115 (≠ G141), D115 (≠ G141), G166 (= G191), E222 (≠ W245), W240 (≠ T264), W240 (≠ T264), G277 (vs. gap), W278 (vs. gap), W278 (vs. gap)
binding magnesium ion: E290 (= E310)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 11, 14, 46, 67, 316, 349
binding magnesium ion: 59, 60, 62

3k02A Crystal structures of the gach receptor of streptomyces glaucescens gla.O in the unliganded form and in complex with acarbose and an acarbose homolog. Comparison with acarbose-loaded maltose binding protein of salmonella typhimurium. (see paper)
28% identity, 68% coverage: 46:333/423 of query aligns to 21:308/388 of 3k02A

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L

L

binding 4,6-dideoxy-4-{[(1S,2R,3R,4S,5S)-2,3,4-trihydroxy-5-(hydroxymethyl)cyclohexyl]amino}-alpha-D-allopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranose: F43 (= F64), E67 (≠ D90), W70 (≠ N93), D117 (≠ G141), D164 (≠ N193), Y166 (≠ W195), W167 (≠ R196), W238 (= W269), W274 (vs. gap)

Sites not aligning to the query:

binding 4,6-dideoxy-4-{[(1S,2R,3R,4S,5S)-2,3,4-trihydroxy-5-(hydroxymethyl)cyclohexyl]amino}-alpha-D-allopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranose: 11, 16, 342, 349

3jzjA Crystal structures of the gach receptor of streptomyces glaucescens gla.O in the unliganded form and in complex with acarbose and an acarbose homolog. Comparison with acarbose-loaded maltose binding protein of salmonella typhimurium. (see paper)
28% identity, 68% coverage: 46:333/423 of query aligns to 21:308/388 of 3jzjA

query
sites
3jzjA

K

Q

S

A

L

L

A

A

D

E

Y

G

Q

F

K

E

R

K

S

E

G

H

T

P

R

K

I

V

E

D

I

V

S

K

Y

Y

-

V

-

N

-

V

-

P

F
|
F

G

G

E

E

N

A

D

N

R

-

A

A

K

K

S

F

R

K

L

N

D

A

A

A

A

G

T

G

K

N

A

S

G

G

S

A

Y

P

Q

D

V

V

Y

M

Y
x
R

V

T

D
x
E

E

V

A

A

N
x
W

V

V

A

A

E

D

F

F

A

A

S

S

A

I

G

G

W

Y

I

L

V

A

P

P

L

L

L

-

K

D

Y

G

F

T

P

P

K

A

D

L

A

D

D

D

Y

G

D

S

D

D

F

H

L

L

P

P

G

Q

R

A

A

A

V

S

A

T

S

R

Y

Y

K

E

D

G

V

K

A

T

Y

Y

F

A

A

V

P

P

L

Q

I

V

G

I

G
x
D

S

T

D

L

F

A

L

L

F

F

Y

Y

R

N

R

K

D

E

L

L

E

T

K

K

A

A

G

G

L

V

P

E

V

V

P

P

R

G

T

S

L

V

D

A

E

E

L

L

V

K

A

T

D

A

I

A

K

A

K

E

L

I

H

T

S

E

P

K

P

T

S

G

V

A

Y

T

G

G

W

L

V

Y

A

L

R

R

G

G

Q

D

R

-

G

-

S

-

G

-

M

-

N
x
D

V

P

W
x
Y

R

W

W

F

A

L

P

P

F

Y

M

L

L

Y

A

G

Q

E

G

G

Q

D

W

L

T

V

D

D

-

E

K

K

N

N

N

K

Q

T

P

V

A

T

F

V

N

D

S

D

P

E

A

A

A

G

V

V

K

R

A

A

T

Y

Q

R

L

V

Y

I

A

K

D

D

L

L

F

V

-

D

K

S

Y

K

A

A

P

A

P

I

G

T

A

D

A

A

T

S

Y

D

D

G

W

W

S

N

N

N

A

M

L

Q

E

N

A

A

F

F

R

K

S

S

G

G

K

K

V

V

A

A

F

M

M

M

I

V

E

N

S

G

T

P

P

-

F

-

A

-

D

-

W
|
W

M

A

E

I

D

E

S

D

S

V

K

K

S

A

S

G

V

A

A

R

D

F

K

K

V

D

G

A

Y

G

A

N

R

L

P

G

P

V

A

A

P

P

L

V

P

P

S

A

A

G

A

S

Y

A

G

G

H

Q

G

G

-

S

-

P

-

Q

-

G

-
x
W

-

N

L

L

A

S

I

V

S

Y

A

A

V

-

G

G

A

S

K

K

D

N

E

L

C

D

T

A

R

S

Q

Y

A

A

A

F

G

V

K

K

F

Y

I

M

A

S

F

S

A

A

T

K

G

V

K

Q

E

Q

Q

Q

E

T

Q

T

A

E

R

K

L

L

binding 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose: E67 (≠ D90), W70 (≠ N93), Y166 (≠ W195)
binding alpha-D-glucopyranose: F43 (= F64), R65 (≠ Y88), E67 (≠ D90), D117 (≠ G141), D164 (≠ N193), W238 (= W269), W238 (= W269), W274 (vs. gap), W274 (vs. gap)

Sites not aligning to the query:

1eu8A Structure of trehalose maltose binding protein from thermococcus litoralis (see paper)
28% identity, 65% coverage: 48:322/423 of query aligns to 24:309/407 of 1eu8A

query
sites
1eu8A

L

I

A

A

D

E

Y

F

Q

E

K

K

R

K

-

Y

S

P

G

G

T

V

R

T

I

V

E

E

I

L

S

K

Y

R

F

Q

G

A

E
x
T

N

D

D
x
T

R

E

R

Q

A
x
R

K

R

S

L

R

D

L

L

D

V

A

N

A

A

T

L

K

R

A

G

G

K

S

S

Y

S

Q

D

-

P

-

D

V

V

Y

F

Y

L

V

M

D
|
D

E

V

A

A

N

W

V

L

A

G

E

Q

F

F

A

I

S

A

A

S

G

G

W

W

I

L

V

E

P

P

L

L

L

D

K

D

Y

Y

F

V

P

Q

K

K

D

D

A

N

-

Y

D

D

Y

L

D

S

D

V

F

F

L

F

P

Q

G

S

R

V

R

I

A

N

V

L

A

A

S

D

Y

K

K

Q

D

G

V

G

A

K

Y

L

F

Y

A

A

-

L

P

P

L

V

I
x
Y

G

I

G
x
D

S

A

D

G

F

L

L

L

F

Y

Y

Y

R

R

R

K

D

D

L

L

E

E

K

K

A

Y

G

G

L

Y

P

S

V

K

-

P

P

P

R

E

T

T

L

W

D

Q

E

E

L

L

V

V

A

E

D

M

I

A

K

Q

K

K

L

I

H

Q

S

S

-

G

-

E

-

R

-

E

-

T

P

N

P

P

S

N

V

F

Y

W

G

G

W

F

V

V

A

W

R

Q

G

G

Q

K

R

Q

G

Y

S

E

G
|
G

M

L

N

-

V

V

W

C

R

D

W

F

A

V

P

E

F

Y

M

V

L

Y

A

S

Q

N

G

G

-

G

-

S

-

L

G

G

Q

E

W

F

T

K

D

D

K

G

N

K

N

W

Q

V

P

P

A

T

F

L

N

N

S

K

P

P

A

E

A

N

V

V

K

E

A

A

T

L

Q

Q

L

F

Y

M

A

V

D

D

L

L

-

I

-

H

-

K

F

Y

K

K

Y

I

A

S

P

P

G

N

A

T

A

Y

T

T

Y

E

D

M

W

T

S
x
E

N

E

A

P

L

V

E

R

-

L

A

M

F

F

R

Q

S

Q

G

G

K

N

V

A

A

A

F

F

M

E

I

-

E

R

S

N

T
x
W

P

P

F
x
Y

A

A

D

W

W

G

M

L

E

H

D

N

S

A

S

D

K

D

S

S

S

P

V

V

A

K

D

G

K

K

V

V

G

G

Y

V

A

-

R

-

P

-

A

A

P

P

L

L

P

P

S

H

A

F

A

P

Y

-

G

G

H

H

G

K

L

S

A

A

I

A

S

T

A

L

V
x
G

G
|
G

A
x
W

K

H

D

I

E

G

C

I

T

S

R

K

Q

Y

A

S

A

D

G

N

K

K

F

A

I

L

A

A

F

W

binding alpha-D-glucopyranose: T43 (≠ E66), T45 (≠ D68), R48 (≠ A71), D69 (= D90), Y120 (≠ I139), D122 (≠ G141), G178 (= G191), E238 (≠ S246), W256 (≠ T264), Y258 (≠ F266), G292 (≠ V305), G293 (= G306), W294 (≠ A307)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 12, 16, 363

Q7LYW7 Trehalose/maltose-binding protein MalE; TMBP from Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) (see paper)
27% identity, 65% coverage: 48:322/423 of query aligns to 65:350/450 of Q7LYW7

query
sites
Q7LYW7

L

I

A

A

D

E

Y

F

Q

E

K

K

R

K

-

Y

S

P

G

G

T

V

R

T

I

V

E

E

I

L

S

K

Y

R

F

Q

G

A

E
x
T

N

D

D

T

R

E

R

Q

A
x
R

K

R

S

L

R

D

L

L

D

V

A

N

A

A

T

L

K

R

A

G

G

K

S

S

Y

S

Q

D

-

P

-

D

V

V

Y

F

Y

L

V

M

D
|
D

E

V

A

A

N

W

V

L

A

G

E

Q

F

F

A

I

S

A

A

S

G

G

W

W

I

L

V

E

P

P

L

L

L

D

K

D

Y

Y

F

V

P

Q

K

K

D

D

A

N

-

Y

D

D

Y

L

D

S

D

V

F

F

L

F

P

Q

G

S

R

V

R

I

A

N

V

L

A

A

S

D

Y

K

K

Q

D

G

V

G

A

K

Y

L

F

Y

A

A

-

L

P

P

L

V

I
x
Y

G

I

G
x
D

S

A

D

G

F

L

L

L

F

Y

Y

Y

R

R

R

K

D

D

L

L

E

E

K

K

A

Y

G

G

L

Y

P

S

V

K

-

P

P

P

R

E

T

T

L

W

D

Q

E

E

L

L

V

V

A

E

D

M

I

A

K

Q

K

K

L

I

H

Q

S

S

-

G

-

E

-

R

-

E

-

T

P

N

P

P

S

N

V

F

Y

W

G

G

W

F

V

V

A

W

R

Q

G

G

Q

K

R

Q

G
x
Y

S

E

G

S

M

L

N

-

V

V

W

C

R

D

W

F

A

V

P

E

F

Y

M

V

L

Y

A

S

Q

N

G

G

-

G

-

S

-

L

G

G

Q

E

W

F

T

K

D

D

K

G

N

K

N

W

Q

V

P

P

A

T

F

L

N

N

S

K

P

P

A

E

A

N

V

V

K

E

A

A

T

L

Q

Q

L

F

Y

M

A

V

D

D

L

L

-

I

-

H

-

K

F

Y

K

K

Y

I

A

S

P

P

G

N

A

T

A

Y

T

T

Y

E

D

M

W

T

S
x
E

N

E

A

P

L

V

E

R

-

L

A

M

F

F

R

Q

S

Q

G

G

K

N

V

A

A

A

F

F

M

E

I

-

E

R

S

N

T
x
W

P

P

F
x
Y

A

A

D

W

W

G

M

L

E

H

D

N

S

A

S

D

K

D

S

S

S

P

V

V

A

K

D

G

K

K

V

V

G

G

Y

V

A

-

R

-

P

-

A

A

P

P

L

L

P

P

S

H

A

F

A

P

Y

-

G

G

H

H

G

K

L

S

A

A

I

A

S

T

A

L

V

G

G
|
G

A
x
W

K

H

D

I

E

G

C

I

T

S

R

K

Q

Y

A

S

A

D

G

N

K

K

F

A

I

L

A

A

F

W

T84 (≠ E66) binding
R89 (≠ A71) binding
D110 (= D90) binding
Y161 (≠ I139) binding
D163 (≠ G141) binding
Y217 (≠ G189) binding
E279 (≠ S246) binding
W297 (≠ T264) binding
Y299 (≠ F266) binding
G334 (= G306) binding
W335 (≠ A307) binding

Sites not aligning to the query:

57 binding
371 binding
404 binding

5ci5A Crystal structure of an abc transporter solute binding protein from thermotoga lettingae tmo (tlet_1705, target efi-510544) bound with alpha-d-tagatose
24% identity, 83% coverage: 48:398/423 of query aligns to 23:372/393 of 5ci5A

query
sites
5ci5A

L

I

A

K

D

T

Y

F

Q

E

K

E

R

K

S

Y

G

G

T

I

R

S

I

V

E

E

I

V

S

Q

Y

L

F

F

G

G

E
x
F

N

D

D

T

R

Y

A

D

K

K

S

-

R

-

L

L

D

V

A

T

A

A

T

L

K

Q

A

A

G

G

S

K

Y

G

-

P

Q

D

V

L

Y

A

Y

F

V

A

D
|
D

E

L

A

G

N

G

-

W

V

V

A

P

E

T

F

F

A

A

S

E

A

K

G

G

W

W

I

L

V

E

P

P

L

M

L

E

K

E

Y

H

F

L

P

K

K

N

D

W

A

E

D

G

Y

T

D

A

D

Q

F

I

L

W

P

P

G

N

R

L

R

W

A

P

V

T

A

V

S

T

Y

Y

K

K

D

K

V

I

A

R

Y

Y

F

G

A

L

P

P

L

W

I
x
Y

G

T

G
x
D

S

C

D
x
R

F

L

L

L

F

L

Y

Y

R

N

R

K

D

A

L

M

L

F

E

E

K

K

A

A

G

G

L

L

-

N

-

P

-

D

P

N

V

P

P

P

R

K

T

T

L

W

D

D

E

E

L

L

V

L

-

D

A

A

D

A

I

L

K

K

K

I

L

T

H

D

S

T

P

K

P

N

S

R

V

I

Y

Y

G

G

W

Y

V

G

A

V

R

S

G

G

Q

T

R

K

G

T

S

E

G

H

M

T

N

T

V

L

W

-

R

G

W

Y

A

M

P

M

F

F

M

L

L

Y

A

A

Q

A

G

G

Q

K

-

L

W

L

T

T

D

D

K

D

N

Y

N

S

Q

K

P

A

A

A

F

F

N

D

S

S

P

P

A

E

A

G

V

L

K

K

A

A

T

L

Q

K

L

F

Y

Y

A

T

D

D

L

L

F

A

K

K

-

K

-

Y

Y

N

A

V

P

S

P

P

G

N

A

A

A

I

T

Q

Y

Y

D

H

W

E

S

D

N

D

A

Y

L

R

E

N

A

M

F

M

R

A

S

Q

G

N

K

R

V

V

A

A

F

M

M

A

I

I

E

-

S

G

T

G

P

P

F
x
W

A

S

D

F

W

P

M

L

E

I

D

E

S

A

K

N

S

P

S

D

V

I

A

A

D

G

K

K

V

Y

G

S

Y

V

A

A

R

L

P

H

P

P

A

Y

P

D

L

A

P

K

S

P

A

A

A

S

Y

V

G

L

H
x
G

G
|
G

L
x
W

A

A

I

L

S

-

A

-

V

V

G

I

A

P

K

S

D

S

E

S

C

P

T

N

R

K

Q

E

A

D

A

A

G

W

K

K

F

L

I

A

A

E

F

Y

A

L

T

T

G

S

K

F

E

D

Q

V

E

W

Q

M

A

K

R

W

L

V

R

E

E

E

K

K

V

G

F

G

S

P

D

M

Y

P

N

T

R

R

S

M

S

D

T

V

I

C

Q

K

S

K

D

S

Y

K

F

L

Q

A

K

N

N

D

V

V

K

K

P

W

Q

Q

I

I

L

I

A

F

G

-

L

-

K

-

D

E

T

T

T

F

P

P

V

H

S

A

K

V

L

A

T
x
R

I

P

W

-

P

P

S

I

P

P

Q

Q

W

Y

P

P

D

Q

I

I

G

S

D

E

N

Q

L

I

G

Q

V

T

A

M

L

V

E

Q

E

R

V

V

F

L

T

L

G

G

binding alpha-D-tagatopyranose: F41 (≠ E66), D64 (= D90), Y114 (≠ I139), D116 (≠ G141), R118 (≠ D143), W246 (≠ F266), G278 (≠ H298), G279 (= G299), W280 (≠ L300), R349 (≠ T374)

Sites not aligning to the query:

binding alpha-D-tagatopyranose: 11

A9CEY9 Sulfoquinovosyl glycerol-binding protein SmoF; SQGro-binding protein SmoF; SQ monooxygenase cluster protein F from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see 2 papers)
26% identity, 66% coverage: 11:289/423 of query aligns to 10:291/416 of A9CEY9

query
sites
A9CEY9

A

A

V

L

L

M

G

G

A

A

L

L

P

C

-

A

-

T

-

M

L

L

V

T

A

F

S

S

A

G

Q

Q

S

A

C

F

N

A

V

D

P

A

V

E

L

L

K

K

V

I

L

F

A

V

Q

S

K

S

S
x
Q

L
x
H

G

Q

L

P

T

D

V

I

M

W

E

R

K

K

S

A

L

L

A

D

D

Q

Y

Y

Q

E

K

A

R

K

S

T

-

P

G

G

T

V

R

K

I

V

E

V

I

I

S

E

Y

T

F

G

G

G

E

N

N

T

D
x
S

R

E

R

M

A

Q

K

A

S

Q

R

Y

L

L

D

N

A

T

-

V

-

M

A

S

T

A

K

K

A

D

G

S

S

S

Y

L

Q

D

V

V

Y

L

Y

M

V

L

D
|
D

E

V

A

I

N

R

V

P

A

A

E

Q

F

F

A

A

S

T

A

A

G

G

W

W

I

T

V

S

P

D

L

F

L

-

K

-

Y

-

F

-

P

-

K

S

D

G

A

K

D

D

Y

L

D

S

D

A

F

Y

L

L

P

P

G

T

R

Y

R

A

A

E

V

A

A

N

S

T

Y

V

K

N

D

G

V

K

A

I

Y

V

F

A

A

L

P

P

L

A

I

F

G

A

G
x
D

S

S

D

M

F

F

L

L

F

Y

Y

Y

R

R

R

K

D

D

L

L

E

D

K

K

A

Y

G

G

L

I

P

K

V

P

P

P

R

T

T

T

L

W

D

D

E

E

L

L

V

K

A

E

D

A

I

S

K

K

L

V

-

M

-

E

-

G

H

E

S

K

P

N

P

P

S

E

V

L

Y

Q

G

G

W

L

V

S

A

F

R

Q

G

G

Q

K

R

A

G

I

S

E

G
|
G

M

A

N

V

V

C

W

T

R

F

W

L

A

L

P

P

F

Y

M

W

L

-

A

-

Q

S

G

E

G

G

Q

K

W

S

T

L

D

V

K

E

N

N

N

G

Q

K

P

L

A

N

F

F

N

D

S

N

P

K

A

A

V

V

K

D

A

S

T

L

Q

K

L

L

Y

W

A

K

D

S

L

F

F

V

K

D

-

D

-

G

Y

I

A

S

P

K

G

N

A

I

A

S

T

E

Y

V

D

A

W
x
T

S

D

N

D

A

T

L

R

E

K

A

E

F

F

R

Q

S

A

G

G

K

K

V

V

A

L

F

F

M

A

I

V

E

N

S

W

T

S

P

-

F

Y

A

A

D

W

W

T

M

H

E

F

D

Q

S

G

S

K

K

E

S

S

S

Q

V

V

A

N

D

D

K

K

V

V

G

G

Y

V

A

A

R

R

P

L

P

P

A

A

Q40 (≠ S38) binding
H41 (≠ L39) binding
S71 (≠ D68) binding
D95 (= D90) binding
D141 (≠ G141) binding
G194 (= G191) binding
T248 (≠ W245) binding

Sites not aligning to the query:

303 binding ; binding
304 binding ; binding
373 binding ; binding

7qhvAAA Sulfoquinovosyl binding protein (see paper)
26% identity, 61% coverage: 31:289/423 of query aligns to 4:261/390 of 7qhvAAA

query
sites
7qhvAAA

L

L

K

K

V

I

L

F

A

V

Q
x
S

K
x
S

S

Q

L

H

G

Q

L

P

T

D

V

I

M

W

E

R

K

K

S

A

L

L

A

D

D

Q

Y

Y

Q

E

K

A

R

K

S

T

-

P

G

G

T

V

R

K

I

V

E

V

I

I

S

E

Y

T

F

G

G

G

E
x
N

N
x
T

D
x
S

R

E

R

M

A

Q

K

A

S

Q

R

Y

L

L

D

N

A

T

-

V

-

M

A

S

T

A

K

K

A

D

G

S

S

S

Y

L

Q

D

V

V

Y

L

Y

M

V

L

D
|
D

E

V

A

I

N

R

V

P

A

A

E

Q

F

F

A

A

S

T

A

A

G

G

W

W

I

T

V

S

P

D

L

F

L

S

K

K

Y

-

F

-

P

-

K

-

D

-

A

-

D

D

Y

L

D

S

D

A

F

Y

L

L

P

P

G

T

R

Y

R

A

A

E

V

A

A

N

S

T

Y

V

K

N

D

G

V

K

A

I

Y

V

F

A

A

L

P

P

L

A

I
x
F

G

A

G
x
D

S

S

D

M

F

F

L

L

F

Y

Y

Y

R

R

R

K

D

D

L

L

E

D

K

K

A

Y

G

G

L

I

P

K

V

P

P

P

R

T

T

T

L

W

D

D

E

E

L

L

V

K

A

E

D

A

I

S

K

K

L

V

-

M

-

E

-

G

H

E

S

K

P

N

P

P

S

E

V

L

Y

Q

G

G

W

L

V

S

A

F

R

Q

G

G

Q

K

R

A

G

I

S
x
E

G
|
G

M

A

N

V

V

C

W

T

R

F

W

L

A

L

P

P

F

Y

M

W

L

-

A

-

Q

S

G

E

G

G

Q

K

W

S

T

L

D

V

K

E

N

N

N

G

Q

K

P

L

A

N

F

F

N

D

S

N

P

K

A

A

V

V

K

D

A

S

T

L

Q

K

L

L

Y

W

A

K

D

S

L

F

F

V

K

D

-

D

-

G

Y

I

A

S

P

K

G

N

A

I

A

S

T

E

Y

V

D
x
A

W
x
T

S
x
D

N

D

A

T

L

R

E

K

A

E

F

F

R

Q

S

A

G

G

K

K

V

V

A

L

F

F

M

A

I

V

E

N

S
x
W

T

S

P

-

F

Y

A

A

D

W

W

T

M

H

E

F

D

Q

S

G

S

K

K

E

S

S

S

Q

V

V

A

N

D

D

K

K

V

V

G

G

Y

V

A

A

R

R

P

L

P

P

A

A

binding [(2~{S},3~{S},4~{S},5~{R},6~{S})-6-[(2~{S})-3-butanoyloxy-2-heptanoyloxy-propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: S9 (≠ Q36), S10 (≠ K37), N40 (≠ E66), T41 (≠ N67), S42 (≠ D68), D66 (= D90), F109 (≠ I139), D111 (≠ G141), E163 (≠ S190), G164 (= G191), A217 (≠ D244), T218 (≠ W245), D219 (≠ S246), W236 (≠ S263)

Sites not aligning to the query:

binding [(2~{S},3~{S},4~{S},5~{R},6~{S})-6-[(2~{S})-3-butanoyloxy-2-heptanoyloxy-propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 272, 273, 274, 343

7yzsAAA Sulfoquinovosyl binding protein (see paper)
25% identity, 61% coverage: 31:289/423 of query aligns to 3:261/384 of 7yzsAAA

query
sites
7yzsAAA

L

L

K

K

V

I

L

F

A

V

Q

S

K

S

S

Q

L
x
H

G

Q

L

P

T

D

V

I

M

W

E

R

K

K

S

A

L

L

A

D

D

Q

Y

Y

Q

E

K

A

R

K

S

T

-

P

G

G

T

V

R

K

I

V

E

V

I

I

S

E

Y

T

F

G

G

G

E

N

N
x
T

D
x
S

R

E

R

M

A

Q

K

A

S

Q

R

Y

L

L

D

N

A

T

-

V

-

M

A

S

T

A

K

K

A

D

G

S

S

S

Y

L

Q

D

V

V

Y

L

Y

M

V

L

D
|
D

E

V

A

I

N

R

V

P

A

A

E

Q

F

F

A

A

S

T

A

A

G

G

W

W

I

T

V

S

P

D

L

F

L

-

K

-

Y

-

F

-

P

-

K

S

D

G

A

K

D

D

Y

L

D

S

D

A

F

Y

L

L

P

P

G

T

R

Y

R

A

A

E

V

A

A

N

S

T

Y

V

K

N

D

G

V

K

A

I

Y

V

F

A

A

L

P

P

L

A

I

F

G

A

G
x
D

S

S

D

M

F

F

L

L

F

Y

Y

Y

R

R

R

K

D

D

L

L

E

D

K

K

A

Y

G

G

L

I

P

K

V

P

P

P

R

T

T

T

L

W

D

D

E

E

L

L

V

K

A

E

D

A

I

S

K

K

L

V

-

M

-

E

-

G

H

E

S

K

P

N

P

P

S

E

V

L

Y

Q

G

G

W

L

V

S

A

F

R

Q

G

G

Q

K

R

A

G

I

S
x
E

G
|
G

M

A

N

V

V

C

W

T

R

F

W

L

A

L

P

P

F

Y

M

W

L

-

A

-

Q

S

G

E

G

G

Q

K

W

S

T

L

D

V

K

E

N

N

N

G

Q

K

P

L

A

N

F

F

N

D

S

N

P

K

A

A

V

V

K

D

A

S

T

L

Q

K

L

L

Y

W

A

K

D

S

L

F

F

V

K

D

-

D

-

G

Y

I

A

S

P

K

G

N

A

I

A

S

T

E

Y

V

D

A

W
x
T

S

D

N

D

A

T

L

R

E

K

A

E

F

F

R

Q

S

A

G

G

K

K

V

V

A

L

F

F

M

A

I

V

E

N

S
x
W

T

S

P

-

F

Y

A

A

D

W

W

T

M

H

E

F

D

Q

S

G

S

K

K

E

S

S

S

Q

V

V

A

N

D

D

K

K

V

V

G

G

Y

V

A

A

R

R

P

L

P

P

A

A

binding 6-deoxy-6-sulfo-beta-D-glucopyranose: H11 (≠ L39), T40 (≠ N67), S41 (≠ D68), D65 (= D90), D111 (≠ G141), E163 (≠ S190), G164 (= G191), T218 (≠ W245), W236 (≠ S263)

Sites not aligning to the query:

binding 6-deoxy-6-sulfo-beta-D-glucopyranose: 272, 273, 274, 343

7ofyA Crystal structure of sq binding protein from agrobacterium tumefaciens in complex with sulfoquinovosyl glycerol (sqgro) (see paper)
25% identity, 61% coverage: 31:289/423 of query aligns to 5:263/389 of 7ofyA

query
sites
7ofyA

L

L

K

K

V

I

L

F

A

V

Q

S

K

S

S
x
Q

L

H

G

Q

L

P

T

D

V

I

M

W

E

R

K

K

S

A

L

L

A

D

D

Q

Y

Y

Q

E

K

A

R

K

S

T

-

P

G

G

T

V

R

K

I

V

E

V

I

I

S

E

Y

T

F

G

G

G

E

N

N
x
T

D
x
S

R

E

R

M

A

Q

K

A

S

Q

R

Y

L

L

D

N

A

T

-

V

-

M

A

S

T

A

K

K

A

D

G

S

S

S

Y

L

Q

D

V

V

Y

L

Y

M

V

L

D
|
D

E

V

A

I

N

R

V

P

A

A

E

Q

F

F

A

A

S

T

A

A

G

G

W

W

I

T

V

S

P

D

L

F

L

-

K

-

Y

-

F

-

P

-

K

S

D

G

A

K

D

D

Y

L

D

S

D

A

F

Y

L

L

P

P

G

T

R

Y

R

A

A

E

V

A

A

N

S

T

Y

V

K

N

D

G

V

K

A

I

Y

V

F

A

A

L

P

P

L

A

I

F

G

A

G
x
D

S

S

D

M

F

F

L

L

F

Y

Y

Y

R

R

R

K

D

D

L

L

E

D

K

K

A

Y

G

G

L

I

P

K

V

P

P

P

R

T

T

T

L

W

D

D

E

E

L

L

V

K

A

E

D

A

I

S

K

K

L

V

-

M

-

E

-

G

H

E

S

K

P

N

P

P

S

E

V

L

Y

Q

G

G

W

L

V

S

A

F

R

Q

G

G

Q

K

R

A

G

I

S

E

G
|
G

M

A

N

V

V

C

W

T

R

F

W

L

A

L

P

P

F

Y

M

W

L

-

A

-

Q

S

G

E

G

G

Q

K

W

S

T

L

D

V

K

E

N

N

N

G

Q

K

P

L

A

N

F

F

N

D

S

N

P

K

A

A

V

V

K

D

A

S

T

L

Q

K

L

L

Y

W

A

K

D

S

L

F

F

V

K

D

-

D

-

G

Y

I

A

S

P

K

G

N

A

I

A

S

T

E

Y

V

D

A

W
x
T

S

D

N

D

A

T

L

R

E

K

A

E

F

F

R

Q

S

A

G

G

K

K

V

V

A

L

F

F

M

A

I

V

E

N

S
x
W

T

S

P

-

F
x
Y

A

A

D

W

W

T

M

H

E

F

D

Q

S

G

S

K

K

E

S

S

S

Q

V

V

A

N

D

D

K

K

V

V

G

G

Y

V

A

A

R

R

P

L

P

P

A

A

binding [(2S,3S,4S,5R,6S)-6-[(2R)-2,3-bis(oxidanyl)propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: Q12 (≠ S38), T42 (≠ N67), S43 (≠ D68), D67 (= D90), D113 (≠ G141), G166 (= G191), T220 (≠ W245), W238 (≠ S263), Y240 (≠ F266)

Sites not aligning to the query:

binding [(2S,3S,4S,5R,6S)-6-[(2R)-2,3-bis(oxidanyl)propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 274, 275, 276, 345

7yzuA Crystal structure of the sulfoquinovosyl binding protein smof complexed with sqme (see paper)
26% identity, 61% coverage: 31:289/423 of query aligns to 2:258/382 of 7yzuA

query
sites
7yzuA

L

L

K

K

V

I

L

F

A

V

Q

S

K

S

S
x
Q

L

H

G

Q

L

P

T

D

V

I

M

W

E

R

K

K

S

A

L

L

A

D

D

Q

Y

Y

Q

E

K

A

R

K

S

T

-

P

G

G

T

V

R

K

I

V

E

V

I

I

S

E

Y

T

F

G

G

G

E

N

N
x
T

D
x
S

R

E

R

M

A

Q

K

A

S

Q

R

Y

L

L

D

N

A

T

-

V

-

M

A

S

T

A

K

K

A

D

G

S

S

S

Y

L

Q

D

V

V

Y

L

Y

M

V

L

D
|
D

E

V

A

I

N

R

V

P

A

A

E

Q

F

F

A

A

S

T

A

A

G

G

W

W

I

T

V

S

P

D

L

F

L

-

K

-

Y

-

F

-

P

-

K

S

D

G

A

K

D

D

Y

L

D

S

D

A

F

Y

L

L

P

P

G

T

R

Y

R

A

A

E

V

A

A

N

S

T

Y

V

K

N

D

G

V

K

A

I

Y

V

F

A

A

L

P

P

L

A

I

F

G

A

G
x
D

S

S

D

M

F

F

L

L

F

Y

Y

Y

R

R

R

K

D

D

L

L

E

D

K

K

A

Y

G

G

L

I

P

K

V

P

P

P

R

T

T

T

L

W

D

D

E

E

L

L

V

K

A

E

D

A

I

S

K

K

L

V

-

M

-

E

-

G

H

E

S

K

P

N

P

P

S

E

V

L

Y

Q

G

G

W

L

V

S

A

F

R

Q

G

G

Q

K

R

A

G

I

S
x
E

G
|
G

M

A

N

V

V

C

W

T

R

F

W

L

A

L

P

P

F

Y

M

W

L

-

A

S

Q

E

G

G

G

K

Q

S

W

L

T

V

D

G

K

K

N

L

N

N

Q

-

P

-

A

-

F

F

N

D

S

N

P

K

A

A

V

V

K

D

A

S

T

L

Q

K

L

L

Y

W

A

K

D

S

L

F

F

V

K

D

-

D

-

G

Y

I

A

S

P

K

G

N

A

I

A

S

T

E

Y

V

D

A

W
x
T

S

D

N

D

A

T

L

R

E

K

A

E

F

F

R

Q

S

A

G

G

K

K

V

V

A

L

F

F

M

A

I

V

E

N

S
x
W

T

S

P

-

F

Y

A

A

D

W

W

T

M

H

E

F

D

Q

S

G

S

K

K

E

S

S

S

Q

V

V

A

N

D

D

K

K

V

V

G

G

Y

V

A

A

R

R

P

L

P

P

A

A

binding [(2S,3S,4S,5R,6S)-6-methoxy-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: Q9 (≠ S38), T39 (≠ N67), S40 (≠ D68), D64 (= D90), D110 (≠ G141), E162 (≠ S190), G163 (= G191), T215 (≠ W245), W233 (≠ S263)

Sites not aligning to the query:

binding [(2S,3S,4S,5R,6S)-6-methoxy-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 269, 270, 271, 340

G7CES0 Trehalose-binding lipoprotein LpqY; Extracellular solute-binding protein; SugABC transporter substrate-binding protein LpqY; SugABC transporter SBP LpqY from Mycolicibacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316) (Mycobacterium thermoresistibile) (see paper)
28% identity, 42% coverage: 83:261/423 of query aligns to 93:277/471 of G7CES0

query
sites
G7CES0

S

S

Y

L

Q

D

V

V

Y

M

Y

A

V

L

D
|
D

E

V

A

V

N

W

V

T

A

A

E

E

F

F

A

A

S

E

A

A

G

G

W

W

I

A

V

L

P

P

L

L

L

S

K

E

Y

D

F

P

P

A

K

G

D

L

A

A

D

E

Y

A

D

D

-

A

-

T

D

N

F

T

L

L

P

P

G

G

R

P

R

L

A

E

V

T

A

A

S

K

Y

W

K

N

D

G

V

E

A

L

Y

Y

F

A

A

A

P

P

L

I

I

T

G

T

G
x
N

S

T

D

Q

F

L

L

L

F

W

Y

Y

R

R

R

A

D

D

L

L

L

M

E

D

K

E

A

P

G

-

L

-

P

-

V

-

P

P

R

A

T

T

L

W

D

D

E

E

L

M

V

L

A

S

D

E

I

A

K

A

K

R

L

L

H

H

S

A

P

Q

-

G

P

P

S

S

V

-

Y

-

G

-

W

W

V

I

A

A

-

V

R

Q

G

G

Q

K

R

Q

G
x
Y

S

E

G

G

M

L

N

V

V

V

W

W

R

-

W

F

A

N

P

T

F

L

M

L

L

E

A

S

Q

A

G

G

Q

Q

W

V

T

L

D

S

K

D

N

D

N

G

Q

Q

P

R

A

V

-

T

-

L

F

T

N

D

S

T

P

P

A

E

A

H

V

R

K

A

A

A

T

T

Q

V

L

K

Y

A

A

L

D

E

L

I

F

I

K

K

Y

A

-

V

-

A

-

T

-

A

-

P

-

G

A

A

P

D

P

P

G

S

A

I

A

T

T

Q

Y

T

D

D

W
x
E

S

N

N

T

A

A

L

R

E

L

A

A

F

L

R

E

S

Q

G

G

K

R

V

A

A

A

F

L

M

E

I

V

D100 (= D90) binding ; mutation to A: Structurally unstable.
N154 (≠ G141) binding ; mutation to A: About 100-fold increase in constant dissociation (Kd) value of trehalose compared to wild-type.
Y198 (≠ G189) binding
E261 (≠ W245) mutation to A: Loss of trehalose-binding.

Sites not aligning to the query:

45 binding ; N→A: About 3-fold increase in constant dissociation (Kd) value of trehalose compared to wild-type.; N→T: Increased trehalose-binding; when associated with D-46.
46 binding ; E→A: About 3-fold increase in constant dissociation (Kd) value of trehalose compared to wild-type.; E→D: Increased trehalose-binding; when associated with T-45.
57 modified: Disulfide link with 375
79 binding ; Q→A: About 10-fold increase in constant dissociation (Kd) value of trehalose compared to wild-type.
279 binding ; W→A: Loss of trehalose-binding.
281 binding
354 binding
355 L→A: About 13-fold increase in constant dissociation (Kd) value of trehalose compared to wild-type.
375 modified: Disulfide link with 57
424 binding ; R→A: Loss of trehalose-binding.

8ja7E Cryo-em structure of mycobacterium tuberculosis lpqy-sugabc in complex with trehalose
28% identity, 46% coverage: 83:278/423 of query aligns to 65:267/443 of 8ja7E

query
sites
8ja7E

S

T

Y

L

Q

D

V

V

Y

M

Y

A

V

L

D
|
D

E

V

A

V

N

W

V

T

A

A

E

E

F

F

A

A

S

E

A

A

G

G

W

W

I

A

V

L

P

P

L

L

L

S

K

D

Y

D

F

P

P

A

K

G

D

L

A

A

D

E

Y

N

D

D

-

A

-

V

-

A

D

D

F

T

L

L

P

P

G

G

R

P

R

L

A

A

V

T

A

A

S

G

Y

W

K

N

D

H

V

K

A

L

Y

Y

F

A

A

A

P

P

L

V

I

T

G

T

G
x
N

S

T

D

Q

F

L

L

L

F

W

Y

Y

R

R

R

P

D

D

L

L

L

V

E

N

K

S

A

P

G

-

L

-

P

-

V

-

P

P

R

T

T

D

L

W

D

N

E

A

L

M

V

I

A

A

D

E

I

A

K

A

K

R

L

L

H

H

S

A

-

A

-

G

P

E

P

P

S

S

V

-

Y

-

G

-

W

W

V

I

A

A

-

V

R

Q

G

A

Q

N

R

Q

G

G

S

E

G

G

M

L

N

V

V

V

W

W

R

F

W

N

A

T

P

L

F

L

-

V

-

S

-

A

-

G

-

S

M

V

L

L

A

S

Q

E

G

D

G

G

Q

R

W

H

T

V

D

T

K

L

N

T

N

D

Q

T

P

P

A

A

F

H

N

R

S

A

P

-

A

A

A

T

V

V

K

S

A

A

T

L

Q

Q

L

I

Y

L

A

K

D

S

L

V

F

A

K

T

Y

T

-

P

-

G

A

A

P

D

P

P

G

S

A

I

A

T

T

R

Y

T

D

E

W

E

S

G

N

S

A

A

L

R

E

L

A

A

F

F

R

E

S

Q

G

G

K

K

V

A

A

A

F

L

M

E

I

V

E

N

-
x
W

S

P

T
x
F

P

V

F

F

A

A

D

S

W

M

M

L

E

E

D

N

S

A

S

V

K

K

S

G

V

V

binding alpha-D-glucopyranose: D72 (= D90), N126 (≠ G141), W251 (vs. gap), F253 (≠ T264)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 17, 48, 325, 326, 396

P9WGU9 Trehalose-binding lipoprotein LpqY; SugABC transporter substrate-binding protein LpqY; SugABC transporter SBP LpqY from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
28% identity, 46% coverage: 83:278/423 of query aligns to 90:292/468 of P9WGU9

query
sites
P9WGU9

S

T

Y

L

Q

D

V

V

Y

M

Y

A

V

L

D
|
D

E

V

A

V

N

W

V

T

A

A

E

E

F

F

A

A

S

E

A

A

G

G

W

W

I

A

V

L

P

P

L

L

L

S

K

D

Y

D

F

P

P

A

K

G

D

L

A

A

D

E

Y

N

D

D

-

A

-

V

-

A

D

D

F

T

L

L

P

P

G

G

R

P

R

L

A

A

V

T

A

A

S

G

Y

W

K

N

D

H

V

K

A

L

Y

Y

F

A

A

A

P

P

L

V

I

T

G

T

G
x
N

S

T

D

Q

F

L

L

L

F

W

Y

Y

R

R

R

P

D

D

L

L

L

V

E

N

K

S

A

P

G

-

L

-

P

-

V

-

P

P

R

T

T

D

L

W

D

N

E

A

L

M

V

I

A

A

D

E

I

A

K

A

K

R

L

L

H

H

S

A

-

A

-

G

P

E

P

P

S

S

V

-

Y

-

G

-

W

W

V

I

A

A

-

V

R

Q

G

A

Q

N

R

Q

G

G

S

E

G

G

M

L

N

V

V

V

W

W

R

F

W

N

A

T

P

L

F

L

-

V

-

S

-

A

-

G

-

S

M

V

L

L

A

S

Q

E

G

D

G

G

Q

R

W

H

T

V

D

T

K

L

N

T

N

D

Q

T

P

P

A

A

F

H

N

R

S

A

P

-

A

A

A

T

V

V

K

S

A

A

T

L

Q

Q

L

I

Y

L

A

K

D

S

L

V

F

A

K

T

Y

T

-

P

-

G

A

A

P

D

P

P

G

S

A

I

A

T

T

R

Y

T

D

E

W

E

S

G

N

S

A

A

L

R

E

L

A

A

F

F

R

E

S

Q

G

G

K

K

V

A

A

A

F

L

M

E

I

V

E

N

-
x
W

S

P

T
x
F

P

V

F

F

A

A

D

S

W

M

M

L

E

E

D

N

S

A

S

V

K

K

S

G

V

V

D97 (= D90) binding
N151 (≠ G141) binding
W276 (vs. gap) binding
F278 (≠ T264) binding

Sites not aligning to the query:

54 modified: Disulfide link with 372
351 binding
372 modified: Disulfide link with 54
421 binding

7apeA Crystal structure of lpqy from mycobacterium thermoresistible in complex with trehalose (see paper)
28% identity, 42% coverage: 83:261/423 of query aligns to 60:244/435 of 7apeA

query
sites
7apeA

S

S

Y

L

Q

D

V

V

Y

M

Y

A

V

L

D
|
D

E

V

A

V

N

W

V

T

A

A

E

E

F

F

A

A

S

E

A

A

G

G

W

W

I

A

V

L

P

P

L

L

L

S

K

E

Y

D

F

P

P

A

K

G

D

L

A

A

D

E

Y

A

D

D

-

A

-

T

D

N

F

T

L

L

P

P

G

G

R

P

R

L

A

E

V

T

A

A

S

K

Y

W

K

N

D

G

V

E

A

L

Y

Y

F

A

A

A

P

P

L

I

I

T

G

T

G

N

S

T

D

Q

F

L

L

L

F

W

Y

Y

R

R

R

A

D

D

L

L

L

M

E

D

K

E

A

P

G

-

L

-

P

-

V

-

P

P

R

A

T

T

L

W

D

D

E

E

L

M

V

L

A

S

D

E

I

A

K

A

K

R

L

L

H

H

S

A

P

Q

-

G

P

P

S

S

V

-

Y

-

G

-

W

W

V

I

A

A

-

V

R

Q

G

G

Q

K

R

Q

G

Y

S
x
E

G
|
G

M

L

N

V

V

V

W

W

R

-

W

F

A

N

P

T

F

L

M

L

L

E

A

S

Q

A

G

G

Q

Q

W

V

T

L

D

S

K

D

N

D

N

G

Q

Q

P

R

A

V

-

T

-

L

F

T

N

D

S

T

P

P

A

E

A

H

V

R

K

A

A

A

T

T

Q

V

L

K

Y

A

A

L

D

E

L

I

F

I

K

K

Y

A

-

V

-

A

-

T

-

A

-

P

-

G

A

A

P

D

P

P

G

S

A

I

A

T

T

Q

Y

T

D

D

W
x
E

S

N

N

T

A

A

L

R

E

L

A

A

F

L

R

E

S

Q

G

G

K

R

V

A

A

A

F

L

M

E

I

V

binding alpha-D-glucopyranose: D67 (= D90), E166 (≠ S190), G167 (= G191), E228 (≠ W245)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 10, 12, 13, 246, 248, 320, 321, 358, 391

8jadA Crystal structure of mycobacterium tuberculosis lpqy in complex with trehalose analogue yb-17
28% identity, 46% coverage: 83:278/423 of query aligns to 59:261/434 of 8jadA

query
sites
8jadA

S

T

Y

L

Q

D

V

V

Y

M

Y

A

V

L

D
|
D

E

V

A

V

N

W

V

T

A

A

E

E

F

F

A

A

S

E

A

A

G

G

W

W

I

A

V

L

P

P

L

L

L

S

K

D

Y

D

F

P

P

A

K

G

D

L

A

A

D

E

Y

N

D

D

-

A

-

V

-

A

D

D

F

T

L

L

P

P

G

G

R

P

R

L

A

A

V

T

A

A

S

G

Y

W

K

N

D

H

V

K

A

L

Y

Y

F

A

A

A

P

P

L

V

I

T

G

T

G
x
N

S

T

D

Q

F

L

L

L

F

W

Y

Y

R

R

R

P

D

D

L

L

L

V

E

N

K

S

A

P

G

-

L

-

P

-

V

-

P

P

R

T

T

D

L

W

D

N

E

A

L

M

V

I

A

A

D

E

I

A

K

A

K

R

L

L

H

H

S

A

-

A

-

G

P

E

P

P

S

S

V

-

Y

-

G

-

W

W

V

I

A

A

-

V

R

Q

G

A

Q

N

R

Q

G

G

S
x
E

G

G

M

L

N

V

V

V

W

W

R

F

W

N

A

T

P

L

F

L

-

V

-

S

-

A

-

G

-

S

M

V

L

L

A

S

Q

E

G

D

G

G

Q

R

W

H

T

V

D

T

K

L

N

T

N

D

Q

T

P

P

A

A

F

H

N

R

S

A

P

-

A

A

A

T

V

V

K

S

A

A

T

L

Q

Q

L

I

Y

L

A

K

D

S

L

V

F

A

K

T

Y

T

-

P

-

G

A

A

P

D

P

P

G

S

A

I

A

T

T

R

Y

T

D

E

W

E

S

G

N

S

A

A

L

R

E

L

A

A

F

F

R

E

S

Q

G

G

K

K

V

A

A

A

F

L

M

E

I

V

E

N

-
x
W

S

P

T

F

P

V

F

F

A

A

D

S

W

M

M

L

E

E

D

N

S

A

S

V

K

K

S

G

V

V

binding alpha-D-glucopyranose: N120 (≠ G141)
binding 6-amino-6-deoxy-alpha-D-glucopyranose: D66 (= D90), N120 (≠ G141), E165 (≠ S190), W245 (vs. gap)

Sites not aligning to the query:

8jacA Crystal structure of mycobacterium tuberculosis lpqy in complex with trehalose analogue yb-16
28% identity, 46% coverage: 83:278/423 of query aligns to 59:261/434 of 8jacA

query
sites
8jacA

S

T

Y

L

Q

D

V

V

Y

M

Y

A

V

L

D
|
D

E

V

A

V

N

W

V

T

A

A

E

E

F

F

A

A

S

E

A

A

G

G

W

W

I

A

V

L

P

P

L

L

L

S

K

D

Y

D

F

P

P

A

K

G

D

L

A

A

D

E

Y

N

D

D

-

A

-

V

-

A

D

D

F

T

L

L

P

P

G

G

R

P

R

L

A

A

V

T

A

A

S

G

Y

W

K

N

D

H

V

K

A

L

Y

Y

F

A

A

A

P

P

L

V

I

T

G

T

G
x
N

S

T

D

Q

F

L

L

L

F

W

Y

Y

R

R

R

P

D

D

L

L

L

V

E

N

K

S

A

P

G

-

L

-

P

-

V

-

P

P

R

T

T

D

L

W

D

N

E

A

L

M

V

I

A

A

D

E

I

A

K

A

K

R

L

L

H

H

S

A

-

A

-

G

P

E

P

P

S

S

V

-

Y

-

G

-

W

W

V

I

A

A

-

V

R

Q

G

A

Q

N

R

Q

G

G

S
x
E

G
|
G

M

L

N

V

V

V

W

W

R

F

W

N

A

T

P

L

F

L

-

V

-

S

-

A

-

G

-

S

M

V

L

L

A

S

Q

E

G

D

G

G

Q

R

W

H

T

V

D

T

K

L

N

T

N

D

Q

T

P

P

A

A

F

H

N

R

S

A

P

-

A

A

A

T

V

V

K

S

A

A

T

L

Q

Q

L

I

Y

L

A

K

D

S

L

V

F

A

K

T

Y

T

-

P

-

G

A

A

P

D

P

P

G

S

A

I

A

T

T

R

Y

T

D

E

W

E

S

G

N

S

A

A

L

R

E

L

A

A

F

F

R

E

S

Q

G

G

K

K

V

A

A

A

F

L

M

E

I

V

E

N

-
x
W

S

P

T

F

P

V

F

F

A

A

D

S

W

M

M

L

E

E

D

N

S

A

S

V

K

K

S

G

V

V

binding alpha-D-glucopyranose: N120 (≠ G141), W245 (vs. gap)
binding N-[[(2R,3S,4S,5R,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl]ethanamide: D66 (= D90), N120 (≠ G141), E165 (≠ S190), G166 (= G191), W245 (vs. gap)

Sites not aligning to the query:

8ja9A Crystal structure of mycobacterium tuberculosis lpqy in complex with trehalose analogue yb-03
28% identity, 46% coverage: 83:278/423 of query aligns to 59:261/434 of 8ja9A

query
sites
8ja9A

S

T

Y

L

Q

D

V

V

Y

M

Y

A

V

L

D
|
D

E

V

A

V

N

W

V

T

A

A

E

E

F

F

A

A

S

E

A

A

G

G

W

W

I

A

V

L

P

P

L

L

L

S

K

D

Y

D

F

P

P

A

K

G

D

L

A

A

D

E

Y

N

D

D

-

A

-

V

-

A

D

D

F

T

L

L

P

P

G

G

R

P

R

L

A

A

V

T

A

A

S

G

Y

W

K

N

D

H

V

K

A

L

Y

Y

F

A

A

A

P

P

L

V

I

T

G

T

G

N

S

T

D

Q

F

L

L

L

F

W

Y

Y

R

R

R

P

D

D

L

L

L

V

E

N

K

S

A

P

G

-

L

-

P

-

V

-

P

P

R

T

T

D

L

W

D

N

E

A

L

M

V

I

A

A

D

E

I

A

K

A

K

R

L

L

H

H

S

A

-

A

-

G

P

E

P

P

S

S

V

-

Y

-

G

-

W

W

V

I

A

A

-

V

R

Q

G

A

Q

N

R

Q

G

G

S
x
E

G
|
G

M

L

N

V

V

V

W

W

R

F

W

N

A

T

P

L

F

L

-

V

-

S

-

A

-

G

-

S

M

V

L

L

A

S

Q

E

G

D

G

G

Q

R

W

H

T

V

D

T

K

L

N

T

N

D

Q

T

P

P

A

A

F

H

N

R

S

A

P

-

A

A

A

T

V

V

K

S

A

A

T

L

Q

Q

L

I

Y

L

A

K

D

S

L

V

F

A

K

T

Y

T

-

P

-

G

A

A

P

D

P

P

G

S

A

I

A

T

T

R

Y

T

D

E

W

E

S

G

N

S

A

A

L

R

E

L

A

A

F

F

R

E

S

Q

G

G

K

K

V

A

A

A

F

L

M

E

I

V

E

N

-
x
W

S

P

T
x
F

P

V

F

F

A

A

D

S

W

M

M

L

E

E

D

N

S

A

S

V

K

K

S

G

V

V

binding alpha-D-glucopyranose: F247 (≠ T264)
binding (2~{S},3~{R},4~{S},5~{S},6~{S})-6-[(2-azanylhydrazinyl)methyl]oxane-2,3,4,5-tetrol: D66 (= D90), E165 (≠ S190), G166 (= G191), W245 (vs. gap)

Sites not aligning to the query:

Query Sequence

>HSERO_RS17000 FitnessBrowser__HerbieS:HSERO_RS17000
VNKICLGAACAVLGAALPLVASAQSCNVPVLKVLAQKSLGLTVMEKSLADYQKRSGTRIE
ISYFGENDRRAKSRLDAATKAGSYQVYYVDEANVAEFASAGWIVPLLKYFPKDADYDDFL
PGRRAVASYKDVAYFAPLIGGSDFLFYRRDLLEKAGLPVPRTLDELVADIKKLHSPPSVY
GWVARGQRGSGMNVWRWAPFMLAQGGQWTDKNNQPAFNSPAAVKATQLYADLFKYAPPGA
ATYDWSNALEAFRSGKVAFMIESTPFADWMEDSSKSSVADKVGYARPPAPLPSAAYGHGL
AISAVGAKDECTRQAAGKFIAFATGKEQEQARLREKVFSDYNRSSTIQSDYFQKNVKPQI
LAGLKDTTPVSKLTIWPSPQWPDIGDNLGVALEEVFTGTQKDITRALDEAAQYAQDAMEH
ARK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory