SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

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Comparing MCAODC_10750 to proteins with known functional sites using BLASTp with E ≤ 0.001.

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Found 10 hits to proteins with known functional sites (download)

3n6hB Crystal structure of mandelate racemase/muconate lactonizing protein from actinobacillus succinogenes 130z complexed with magnesium/sulfate
74% identity, 96% coverage: 4:73/73 of query aligns to 1:70/432 of 3n6hB

query
sites
3n6hB

Q

Q

S

S

S

V

P

P

V

V

I

I

T

T

D

D

M

M

K

K

V

V

I

I

P

P

V

V

A

A

G

G

Y

H

D

D

S

S

M

M

L

L

L

M

N

N

I

V

G

G

A

A

H

H

N

S

A

P

Y

Y

F

F

T

T

R

R

N

N

I

I

V

V

V

I

L

L

T

T

D

D

N

N

A

S

G

G

H

H

T

T

G

G

I

V

G

G

E

E

A

A

P

P

G

G

E

A

V

T

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I

Y

E

Q

N

T

A

L

L

V

T

D

E

A

A

I

I

P

P

M

H

V

V

L

V

G

G

Sites not aligning to the query:

active site: 189, 191, 219, 221, 244, 273, 297, 323, 325
binding magnesium ion: 219, 244, 273

3pfrA Crystal structure of d-glucarate dehydratase related protein from actinobacillus succinogenes complexed with d-glucarate
74% identity, 95% coverage: 5:73/73 of query aligns to 1:69/426 of 3pfrA

query
sites
3pfrA

S

S

S

V

P

P

V

V

I

I

T

T

D

D

M

M

K

K

V

V

I

I

P

P

V

V

A

A

G

G

Y

H

D

D

S

S

M

M

L

L

L

M

N
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N

I

V

G

G

A

A

H
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H

N

S

A

P

Y

Y

F

F

T

T

R

R

N

N

I

I

V

V

V

I

L

L

T

T

D

D

N

N

A

S

G

G

H

H

T

T

G

G

I

V

G

G

E

E

A

A

P

P

G

G

E

A

V

T

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I

Y

E

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N

T

A

L

L

V

T

D

E

A

A

I

I

P

P

M

H

V

V

L

V

G

G

binding d-glucarate: N22 (= N26), H27 (= H31)

Sites not aligning to the query:

active site: 185, 187, 215, 217, 240, 269, 293, 319, 321
binding d-glucarate: 130, 132, 187, 215, 217, 269, 319, 320, 321, 348
binding magnesium ion: 215, 240, 269

P0AES2 Glucarate dehydratase; GDH; GlucD; D-glucarate dehydratase; EC 4.2.1.40 from Escherichia coli (strain K12) (see 3 papers)
64% identity, 100% coverage: 1:73/73 of query aligns to 1:74/446 of P0AES2

query
sites
P0AES2

M
|
M

T

S

Q

Q

-

F

S

T

P

P

V

V

I

V

T

T

D

E

M

M

K

Q

V

V

I

I

P

P

V

V

A

A

G

G

Y

H

D

D

S

S

M

M

L

L

L

M

N

N

I

L

G

S

G

G

A

A

H

H

N

A

A

P

Y

F

F

F

T

T

R

R

N

N

I

I

V

V

V

I

L

I

T

K

D

D

N

N

A

S

G

G

H

H

T

T

G

G

I

V

G

G

E

E

A

I

P

P

G

G

E

E

V

K

I

I

Y

R

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K

T

T

L

L

V

E

D

D

A

A

I

I

P

P

M

L

V

V

L

V

G

G

M1 (= M1) modified: Initiator methionine, Removed

Sites not aligning to the query:

150 Y→F: Reduces activity 100-fold.
207 active site, Proton acceptor; K→Q: Reduces activity 1000-fold.; K→R: Reduces activity 10000-fold.
235 binding Mg(2+)
266 binding Mg(2+)
289 binding Mg(2+)
339 active site, Proton acceptor; H→A: Loss of activity.; H→N: Reduces activity 10000-fold.; H→Q: Reduces activity 1000-fold.
341 N→D: Inactive in the dehydration reaction of D-glucarate, L-idarate, and 4F-Gluc.; N→L: Almost no effect on the dehydration reaction of D-glucarate, L-idarate, and 4F-Gluc.
366 mutation D->A,N: Reduces activity over 100-fold.

1ecqA E. Coli glucarate dehydratase bound to 4-deoxyglucarate (see paper)
65% identity, 95% coverage: 5:73/73 of query aligns to 4:72/444 of 1ecqA

query
sites
1ecqA

S

T

P

P

V

V

I

V

T

T

D

E

M

M

K

Q

V

V

I

I

P

P

V

V

A

A

G

G

Y

H

D

D

S

S

M

M

L

L

L

M

N
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N

I

L

G

S

G

G

A

A

H
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H

N

A

A

P

Y

F

F

F

T

T

R

R

N

N

I

I

V

V

V

I

L

I

T

K

D

D

N

N

A

S

G

G

H

H

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T

G

G

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G

G

E

E

A

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P

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G

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T

L

L

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E

D

D

A

A

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I

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P

M

L

V

V

L

V

G

G

binding 4-deoxyglucarate: N25 (= N26), H30 (= H31)

Sites not aligning to the query:

active site: 203, 205, 233, 235, 258, 287, 288, 311, 337, 339, 363
binding 4-deoxyglucarate: 101, 148, 150, 205, 233, 235, 287, 337, 338, 339, 366, 420
binding magnesium ion: 233, 258, 287

1ec9D E. Coli glucarate dehydratase bound to xylarohydroxamate (see paper)
65% identity, 95% coverage: 5:73/73 of query aligns to 4:72/444 of 1ec9D

query
sites
1ec9D

S

T

P

P

V

V

I

V

T

T

D

E

M

M

K

Q

V

V

I

I

P

P

V

V

A

A

G

G

Y

H

D

D

S

S

M

M

L

L

L

M

N

N

I

L

G

S

G

G

A

A

H
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H

N

A

A

P

Y

F

F

F

T

T

R

R

N

N

I

I

V

V

V

I

L

I

T

K

D

D

N

N

A

S

G

G

H

H

T

T

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G

E

E

A

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T

L

L

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E

D

D

A

A

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I

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P

M

L

V

V

L

V

G

G

binding xylarohydroxamate: H30 (= H31)

Sites not aligning to the query:

active site: 203, 205, 233, 235, 258, 287, 288, 311, 337, 339, 363
binding magnesium ion: 233, 258, 287
binding xylarohydroxamate: 101, 148, 150, 205, 233, 235, 287, 337, 338, 339, 366, 420

1ec8A E. Coli glucarate dehydratase bound to product 2,3-dihydroxy-5-oxo- hexanedioate (see paper)
65% identity, 95% coverage: 5:73/73 of query aligns to 2:70/442 of 1ec8A

query
sites
1ec8A

S

T

P

P

V

V

I

V

T

T

D

E

M

M

K

Q

V

V

I

I

P

P

V

V

A

A

G

G

Y

H

D

D

S

S

M

M

L

L

L

M

N
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N

I

L

G

S

G

G

A

A

H
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H

N

A

A

P

Y

F

F

F

T

T

R

R

N

N

I

I

V

V

V

I

L

I

T

K

D

D

N

N

A

S

G

G

H

H

T

T

G

G

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G

G

E

E

A

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P

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G

E

E

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T

T

L

L

V

E

D

D

A

A

I

I

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P

M

L

V

V

L

V

G

G

binding 2,3-dihydroxy-5-oxo-hexanedioate: N23 (= N26), H28 (= H31)

Sites not aligning to the query:

active site: 201, 203, 231, 233, 256, 285, 286, 309, 335, 337, 361
binding 2,3-dihydroxy-5-oxo-hexanedioate: 99, 146, 203, 231, 233, 285, 335, 336, 337, 364, 418
binding magnesium ion: 231, 256, 285

1jctA Glucarate dehydratase, n341l mutant orthorhombic form (see paper)
65% identity, 95% coverage: 5:73/73 of query aligns to 3:71/443 of 1jctA

query
sites
1jctA

S

T

P

P

V

V

I

V

T

T

D

E

M

M

K

Q

V

V

I

I

P

P

V

V

A

A

G

G

Y

H

D

D

S

S

M

M

L

L

L

M

N
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N

I

L

G

S

G

G

A

A

H
|
H

N

A

A

P

Y

F

F

F

T

T

R

R

N

N

I

I

V

V

V

I

L

I

T

K

D

D

N

N

A

S

G

G

H

H

T

T

G

G

I

V

G

G

E

E

A

I

P

P

G

G

E

E

V

K

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I

Y

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K

T

T

L

L

V

E

D

D

A

A

I

I

P

P

M

L

V

V

L

V

G

G

binding d-glucarate: N24 (= N26), H29 (= H31)

Sites not aligning to the query:

active site: 202, 204, 232, 234, 257, 286, 287, 310, 336, 338, 362
binding d-glucarate: 100, 147, 149, 204, 232, 286, 337, 419
binding magnesium ion: 232, 257, 286

3p0wB Crystal structure of d-glucarate dehydratase from ralstonia solanacearum complexed with mg and d-glucarate
63% identity, 93% coverage: 6:73/73 of query aligns to 2:69/428 of 3p0wB

query
sites
3p0wB

S

T

P

P

V

R

I

V

T

T

D

E

M

M

K

Q

V

V

I

I

P

P

V

V

A

A

G

G

Y

R

D

D

S

S

M

M

L

L

N

N

I

L

G

C

G

G

A

A

H
|
H

N

A

A

P

Y

F

F

F

T

T

R

R

N

N

I

L

V

V

V

I

L

L

T

K

D

D

N

N

A

A

G

G

H

R

T

T

G

G

I

V

G

G

E

E

A

V

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P

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G

E

E

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G

I

I

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R

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Q

T

A

L

L

V

E

D

R

A

V

I

I

P

P

M

L

V

V

L

V

G

G

binding d-glucarate: H27 (= H31)

Sites not aligning to the query:

active site: 189, 191, 219, 221, 244, 273, 297, 323, 325
binding d-glucarate: 134, 191, 219, 221, 273, 323, 325, 352, 406
binding magnesium ion: 219, 244, 273

3nxlC Crystal structure of glucarate dehydratase from burkholderia cepacia complexed with magnesium
59% identity, 93% coverage: 6:73/73 of query aligns to 2:69/425 of 3nxlC

query
sites
3nxlC

S

T

P

P

V

R

I

V

T

T

D

R

M

M

K

Q

V

V

I

I

P

P

V

V

A

A

G

G

Y

R

D

D

S

S

M

M

L

L

N

N

I

L

G

C

G

G

A

A

H
|
H

N

A

A

P

Y

Y

F

F

T

T

R

R

N

N

I

L

V

V

V

I

L

L

T

D

D

D

N

S

A

S

G

G

H

H

T

T

G

G

I

V

G

G

E

E

A

V

P

P

G

G

E

E

V

G

I

I

Y

R

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H

T

A

L

L

V

E

D

R

A

M

I

T

P

D

M

L

V

V

L

V

G

G

binding carbonate ion: H27 (= H31)

Sites not aligning to the query:

3nfuA Crystal structure of probable glucarate dehydratase from chromohalobacter salexigens dsm 3043 complexed with magnesium
45% identity, 92% coverage: 7:73/73 of query aligns to 3:69/441 of 3nfuA

query
sites
3nfuA

P

P

V

K

I

I

T

T

D

K

M

M

K

N

V

V

I

V

P

P

V

V

A

A

G

G

Y

E

D

D

S

G

M

F

L

L

N

N

I

L

G

S

G

G

A

G

H

H

N

E

A

P

Y

W

F

F

T

I

R

R

N

C

I

V

V

L

V

V

L

L

T

E

D

D

N

E

A

S

G

G

H

N

T

R

G

G

I

V

G

G

E

E

A

I

P

P

G

S

E

E

V

G

I

I

Y

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Q

N

T

G

L

L

V

E

D

K

A

C

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R

P

S

M

L

V

V

L

E

G

G

Sites not aligning to the query:

active site: 201, 203, 231, 233, 256, 285, 309, 335, 337
binding magnesium ion: 231, 233, 256, 257, 285

Query Sequence

>MCAODC_10750
MTTQSSPVITDMKVIPVAGYDSMLLNIGGAHNAYFTRNIVVLTDNAGHTGIGEAPGGEVI
YQTLVDAIPMVLG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory