SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing MMP_RS06755 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

8zqdA Anaerobically isolated active [fefe]-hydrogenase cba5h
45% identity, 98% coverage: 1:51/52 of query aligns to 202:254/626 of 8zqdA

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8zqdA

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binding iron/sulfur cluster: I204 (= I3), C208 (= C7), I209 (≠ V8), G210 (= G9), C211 (= C10), G212 (≠ A11), C214 (= C13), C218 (= C17), P219 (= P18), C222 (≠ A21), I223 (= I22), C238 (= C35), T239 (= T36), H240 (≠ N37), C241 (= C38), G242 (≠ A39), C244 (= C41), C248 (= C45), P249 (= P46), I253 (= I50)

Sites not aligning to the query:

binding dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+): 278, 279, 280, 316, 353, 377, 378, 379, 408, 412, 471, 551, 557
binding iron/sulfur cluster: 354, 409, 551, 552, 553, 557
binding zinc ion: 99, 185, 191, 196

6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
44% identity, 92% coverage: 3:50/52 of query aligns to 3:50/393 of 6fahE

query
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6fahE

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binding iron/sulfur cluster: C7 (= C7), C10 (= C10), C13 (= C13), C17 (= C17), P18 (= P18), C35 (= C35), T36 (= T36), N37 (= N37), C38 (= C38), C41 (= C41), C45 (= C45), A49 (= A49)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 180, 200, 281, 282, 307, 308, 320, 321, 322, 323, 324, 337, 338, 339, 343, 344, 358, 359, 377

8a6tB Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
49% identity, 87% coverage: 6:50/52 of query aligns to 581:627/630 of 8a6tB

query
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8a6tB

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binding iron/sulfur cluster: C582 (= C7), I583 (≠ V8), C585 (= C10), C588 (= C13), C592 (= C17), A596 (= A21), I597 (= I22), I607 (= I30), C612 (= C35), C618 (= C41), C622 (= C45), K624 (≠ L47), A626 (= A49), I627 (= I50)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 31, 33, 36, 82, 85, 86
binding flavin mononucleotide: 201, 227, 230, 355, 535, 536
binding nadp nicotinamide-adenine-dinucleotide phosphate: 320, 337, 340, 341, 342, 433
binding iron/sulfur cluster: 487, 488, 489, 491, 494, 534, 536, 537, 575, 577
binding zinc ion: 471, 558, 564

Query Sequence

>MMP_RS06755
MEILDKCVGCAGCVPFCPVGAISAFGKAEIDTEMCTNCAVCKDYCPLDAISE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory