SitesBLAST

O60086 Probable acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
47% identity, 88% coverage: 2:73/82 of query aligns to 70:141/289 of O60086

query
sites
O60086

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Sites not aligning to the query:

34 modified: Phosphoserine

P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 87% coverage: 3:73/82 of query aligns to 6:76/168 of P9WKJ3

query
sites
P9WKJ3

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K44 (≠ P41) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

A0QUX7 Acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
46% identity, 87% coverage: 3:73/82 of query aligns to 8:78/170 of A0QUX7

query
sites
A0QUX7

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K46 (≠ P41) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

6u9dL Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
46% identity, 88% coverage: 2:73/82 of query aligns to 37:108/255 of 6u9dL

query
sites
6u9dL

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Sites not aligning to the query:

binding adenosine-5'-triphosphate: 191, 192, 197, 198, 199, 211, 214, 214, 240

6vz8F Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
40% identity, 95% coverage: 2:79/82 of query aligns to 4:79/159 of 6vz8F

query
sites
6vz8F

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binding valine: D13 (≠ N11), E14 (= E12), G16 (= G14), M17 (≠ A15), I18 (≠ L16), N31 (= N29), I32 (= I30)

6wo1B Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit (see paper)
46% identity, 88% coverage: 2:73/82 of query aligns to 4:75/197 of 6wo1B

query
sites
6wo1B

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binding valine: E14 (= E12), G16 (= G14), L18 (= L16)

Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
40% identity, 95% coverage: 2:79/82 of query aligns to 76:151/477 of Q9FFF4

query
sites
Q9FFF4

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G88 (= G14) mutation to D: Resistance to valine ihibition.
T119 (= T47) mutation to I: In vat1-1; resistance to valine ihibition.

Sites not aligning to the query:

453 R→Q: In vat1-3; resistance to valine ihibition.

Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
40% identity, 95% coverage: 2:79/82 of query aligns to 86:161/491 of Q93YZ7

query
sites
Q93YZ7

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D95 (≠ N11) binding
G98 (= G14) mutation to E: Increased feedback inhibition by valine/isoleucine.
M99 (≠ A15) binding
I100 (≠ L16) binding
F107 (= F23) mutation to A: Decreased feedback inhibition by leucine and valine/isoleucine.
Y112 (≠ F28) mutation to A: Increased feedback inhibition by leucine.
N113 (= N29) binding ; mutation to A: Decreased feedback inhibition by valine/isoleucine.
I114 (= I30) binding

Sites not aligning to the query:

328 binding
331 G→D: Decreased feedback inhibition by leucine and valine/isoleucine.
332 binding
333 binding
340 F→A: Decreased feedback inhibition by valine/isoleucine.
345 Y→A: No effect.
346 binding ; mutation N->A,H: Decreased feedback inhibition by leucine and increased feedback inhibition by valine/isoleucine.
347 binding

6vz8G Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
45% identity, 87% coverage: 3:73/82 of query aligns to 4:74/159 of 6vz8G

query
sites
6vz8G

H

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binding valine: D12 (≠ N11), G15 (= G14), V16 (≠ A15), L17 (= L16), N30 (= N29), I31 (= I30), V36 (= V35)

5yumA Crystallographic structures of ilvn.Val/ile complexes:conformational selectivity for feedback inhibition of ahass (see paper)
33% identity, 84% coverage: 5:73/82 of query aligns to 6:73/91 of 5yumA

query
sites
5yumA

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binding cobalt (ii) ion: H13 (≠ E12), D69 (= D69)
binding isoleucine: N12 (= N11), H13 (≠ E12), V16 (≠ A15), M17 (≠ L16), C36 (≠ V35)

5yppE Crystal structure of ilvn.Val-1a (see paper)
33% identity, 84% coverage: 5:73/82 of query aligns to 6:73/91 of 5yppE

query
sites
5yppE

L

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N

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binding valine: H13 (≠ E12), M17 (≠ L16), N30 (= N29), V31 (≠ I30), C36 (≠ V35)

Query Sequence

>N515DRAFT_0566 FitnessBrowser__Dyella79:N515DRAFT_0566
MKHTLSILLQNEAGALVRVAGLFSARGFNIDSLTVAATQDPAVSQLTLVMHGADEAVDQL
IKQTRKLVDVIEVSHPAAEALQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory