SitesBLAST
Comparing N515DRAFT_2924 FitnessBrowser__Dyella79:N515DRAFT_2924 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
51% identity, 97% coverage: 12:488/492 of query aligns to 5:456/456 of 5oqtA
- binding alanine: A37 (= A45), I38 (= I46), G40 (= G48), T41 (≠ A49), G42 (= G50), F226 (= F257), A227 (≠ S258), I229 (= I260), V312 (= V344)
- binding cholesterol: A195 (= A219), W199 (≠ K223), Y200 (= Y224)
- binding : E24 (≠ A32), G26 (≠ T34), A27 (= A35), F28 (≠ R36), D29 (≠ H37), M32 (≠ L40), A176 (= A200), R177 (≠ F201), A180 (≠ S204), V181 (≠ I205), A184 (= A208), I185 (= I209), V187 (= V211), A188 (≠ T212), L192 (= L216), A195 (= A219), V196 (≠ F220), H293 (≠ S325), Q294 (≠ L326), W296 (= W328), V297 (≠ L329), F300 (≠ I332)
6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
50% identity, 99% coverage: 4:488/492 of query aligns to 2:458/458 of 6f34A
- binding arginine: A39 (= A45), I40 (= I46), G42 (= G48), T43 (≠ A49), G44 (= G50), E115 (= E121), Y116 (= Y122), A119 (≠ T125), F228 (= F257), A229 (≠ S258), I231 (= I260), V314 (= V344), S318 (≠ M348)
- binding cholesterol: A197 (= A219), W201 (≠ K223), Y202 (= Y224)
- binding : G28 (≠ T34), A29 (= A35), F30 (≠ R36), D31 (≠ H37), M34 (≠ L40), A178 (= A200), R179 (≠ F201), A182 (≠ S204), V183 (≠ I205), A186 (= A208), I187 (= I209), A190 (≠ T212), L193 (= L215), L194 (= L216), A197 (= A219), V198 (≠ F220), H295 (≠ S325), Q296 (≠ L326), W298 (= W328), V299 (≠ L329), F302 (≠ I332)
P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
34% identity, 84% coverage: 23:433/492 of query aligns to 22:438/629 of P30825
- N226 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
23% identity, 92% coverage: 25:475/492 of query aligns to 14:436/461 of P76037
- Y110 (= Y122) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.
P82251 b(0,+)-type amino acid transporter 1; b(0,+)AT1; Glycoprotein-associated amino acid transporter b0,+AT1; Solute carrier family 7 member 9 from Homo sapiens (Human) (see 11 papers)
24% identity, 91% coverage: 43:490/492 of query aligns to 40:468/487 of P82251
- V40 (≠ I43) to M: in CSNU; unknown pathological significance
- IIGSG 43:47 (≠ IIGAG 46:50) binding
- I44 (= I47) to T: in CSNU; type I; dbSNP:rs121908485
- S51 (≠ I54) to F: in CSNU; unknown pathological significance
- P52 (≠ T55) to L: in CSNU; impairs protein stability and dimer formation; dbSNP:rs1198613438
- A70 (≠ V72) to V: in CSNU; partial loss of amino acid transport activity; dbSNP:rs769448665
- Y99 (= Y101) to H: in CSNU; unknown pathological significance
- G105 (= G107) to R: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908480
- W114 (= W116) to R: in CSNU; unknown pathological significance
- I120 (≠ E121) to L: in CSNU; unknown pathological significance
- T123 (≠ F124) to M: in CSNU; partial loss of amino acid transport activity; dbSNP:rs79987078
- V142 (= V165) to A: no effect on amino acid transport activity; dbSNP:rs12150889
- C144 (≠ I170) modified: Interchain (with C-114 in SLC3A1)
- V170 (≠ I196) to M: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908479
- A182 (= A208) to T: in CSNU; type III; partial loss of amino acid transport activity; dbSNP:rs79389353
- G195 (≠ A221) to R: in CSNU; type III; decreased amino acid transport activity; dbSNP:rs121908482
- L223 (≠ I254) to M: slightly decreased amino acid transport activity; dbSNP:rs1007160
- A224 (≠ V255) to V: in CSNU; non-classic type I; dbSNP:rs140873167
- N227 (≠ S258) to D: in CSNU; decreased amino acid transport activity
- W230 (vs. gap) to R: in CSNU; complete loss of amino acid transport activity; mutation to A: Abolishes amino acid transport activity.
- D233 (≠ I260) binding ; mutation to A: Complete loss of amino acid transport activity.
- W235 (≠ F262) mutation to A: Complete loss of amino acid transport activity.
- Q237 (≠ A264) mutation to A: Reduces amino acid transport activity.
- G259 (≠ S286) to R: in CSNU; type III; impairs protein stability and dimer formation; dbSNP:rs121908483
- P261 (≠ I288) to L: in CSNU; types I and III; dbSNP:rs121908486
- S286 (≠ T312) to F: in CSNU; unknown pathological significance; dbSNP:rs755135545
- C321 (≠ L349) mutation to S: Does not affect amino acid transport activity.
- A324 (≠ Q352) to E: in CSNU; unknown pathological significance
- V330 (≠ S358) to M: in CSNU; type III; dbSNP:rs201618022
- A331 (≠ M359) to V: in CSNU; non-classic type I; dbSNP:rs768466784
- R333 (= R361) to Q: in CSNU; decreased amino acid transport activity; dbSNP:rs769576205; to W: in CSNU; severe loss of amino acid transport activity; dbSNP:rs121908484
- A354 (≠ G382) to T: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs939028046
- S379 (≠ A407) mutation to A: Markedly reduces amino acid transport activity.
- A382 (≠ T410) to T: in CSNU; severe loss of amino acid transport activity; dbSNP:rs774878350
- W383 (≠ L411) mutation to A: Complete loss of amino acid transport activity.
- Y386 (≠ F414) mutation to A: Loss of amino acid transport activity.
- K401 (≠ P429) to E: in CSNU; unknown pathological significance; dbSNP:rs760264924
- L426 (≠ M452) to P: in CSNU; unknown pathological significance
Sites not aligning to the query:
- 482 P → L: in CSNU; severe loss of amino acid transport activity; no effect on localization to the apical membrane; dbSNP:rs146815072; mutation P->A,G,S,V: No effect on amino acid transport activity.; mutation P->F,I,M,W: Decreased amino acid transport activity.
P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
25% identity, 89% coverage: 21:460/492 of query aligns to 4:417/457 of P15993
- Y103 (= Y122) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.
6li9B Heteromeric amino acid transporter b0,+at-rbat complex bound with arginine (see paper)
24% identity, 91% coverage: 43:490/492 of query aligns to 11:439/458 of 6li9B
O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
23% identity, 85% coverage: 24:439/492 of query aligns to 2:388/438 of O34739
- C94 (≠ V114) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
- C141 (≠ V165) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
- C168 (≠ L216) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
- C291 (≠ V344) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.
Sites not aligning to the query:
- 415 C→S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.
7nf6B Ovine b0,+at-rbat heterodimer (see paper)
23% identity, 91% coverage: 43:490/492 of query aligns to 12:440/455 of 7nf6B
- binding cholesterol: K124 (≠ G168), T125 (≠ H169), A128 (= A172), V161 (≠ L215), I162 (≠ L216), I165 (≠ A219), S166 (≠ F220), V169 (≠ K223), L170 (≠ Y224), V269 (vs. gap), I335 (≠ V385), I339 (≠ L389)
- binding 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine: V108 (= V152), P111 (= P155), F112 (≠ Q156), L126 (≠ I170), L133 (≠ I184), W275 (= W328), F283 (vs. gap)
6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
27% identity, 83% coverage: 43:449/492 of query aligns to 14:392/433 of 6f2wA