SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing OH686_13630 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

8cd1X phikz014 (see paper)
92% identity, 97% coverage: 2:77/78 of query aligns to 1:76/77 of 8cd1X

query
sites
8cd1X

S
|
S

R
|
R

V

V

C

C

Q

Q

V

V

T

T

G

G

K
|
K

G

G

P
|
P

V

V

T
|
T

G

G

N
|
N

N

N

V
x
I

S
|
S

H

H

A
|
A

N
x
H

N
|
N

K
|
K

T

T

R
|
R

R

R

R
|
R

F
|
F

L
|
L

P
|
P

N
|
N

L

L

Q
|
Q

H

H

H
|
H

R
|
R

F

F

W

W

V

V

E

E

S

S

E

E

K

K

R

R

F

F

V

V

R
|
R

L

L

R
|
R

V

V

S

S

A
|
A

K
|
K

G

G

M

M

R
|
R

I

I

I

I

D

D

K
|
K

R
|
R

G

G

I

I

D

E

V

A

V

V

L

L

S

A

E

D

L

L

R

R

A

A

R

R

G

G

E

E

K

K

binding : S1 (= S2), R2 (= R3), K9 (= K10), P11 (= P12), T13 (= T14), N15 (= N16), I17 (≠ V18), S18 (= S19), A20 (= A21), H21 (≠ N22), N22 (= N23), K23 (= K24), R25 (= R26), R27 (= R28), F28 (= F29), L29 (= L30), P30 (= P31), N31 (= N32), Q33 (= Q34), H35 (= H36), R36 (= R37), R47 (= R48), R49 (= R50), A52 (= A53), K53 (= K54), R56 (= R57), K60 (= K61), R61 (= R62)

8cgvw Tiamulin bound to the 50s subunit (see paper)
84% identity, 97% coverage: 2:77/78 of query aligns to 1:76/77 of 8cgvw

query
sites
8cgvw

S
|
S

R
|
R

V

V

C

C

Q

Q

V

V

T

T

G

G

K
|
K

G

R

P

P

V
|
V

T
|
T

G

G

N
|
N

N

N

V
x
R

S
|
S

H
|
H

A
|
A

N
x
L

N
|
N

K

A

T

T

R
x
K

R
|
R

R
|
R

F
|
F

L
|
L

P
|
P

N
|
N

L

L

Q
x
H

H

S

H
|
H

R
|
R

F

F

W

W

V

V

E

E

S

S

E

E

K

K

R

R

F

F

V

V

R

T

L

L

R
|
R

V

V

S

S

A
|
A

K
|
K

G

G

M
|
M

R
|
R

I

V

I

I

D
|
D

K
|
K

R
x
K

G

G

I

I

D

D

V

T

V

V

L

L

S

A

E

E

L

L

R

R

A

A

R

R

G

G

E

E

K

K

binding : H35 (= H36), D59 (= D60)
binding : S1 (= S2), R2 (= R3), K9 (= K10), V12 (= V13), T13 (= T14), N15 (= N16), R17 (≠ V18), S18 (= S19), H19 (= H20), A20 (= A21), L21 (≠ N22), N22 (= N23), K25 (≠ R26), R26 (= R27), R27 (= R28), F28 (= F29), L29 (= L30), P30 (= P31), N31 (= N32), H33 (≠ Q34), H35 (= H36), R36 (= R37), R49 (= R50), A52 (= A53), K53 (= K54), M55 (= M56), R56 (= R57), K60 (= K61), K61 (≠ R62)

8a3lw 50S ribosomal protein L36 (see paper)
84% identity, 97% coverage: 2:77/78 of query aligns to 1:76/77 of 8a3lw

query
sites
8a3lw

S
|
S

R
|
R

V

V

C

C

Q

Q

V

V

T

T

G

G

K
|
K

G

R

P

P

V

V

T
|
T

G

G

N
|
N

N

N

V
x
R

S
|
S

H
|
H

A
|
A

N
x
L

N
|
N

K

A

T

T

R
x
K

R
|
R

R
|
R

F
|
F

L
|
L

P
|
P

N
|
N

L

L

Q
x
H

H

S

H
|
H

R
|
R

F

F

W

W

V

V

E

E

S

S

E

E

K

K

R

R

F

F

V

V

R

T

L

L

R
|
R

V

V

S

S

A
|
A

K
|
K

G

G

M
|
M

R
|
R

I
x
V

I

I

D

D

K
|
K

R

K

G

G

I

I

D

D

V

T

V

V

L

L

S

A

E

E

L

L

R

R

A

A

R

R

G

G

E

E

K
|
K

binding : S1 (= S2), R2 (= R3), K9 (= K10), T13 (= T14), N15 (= N16), R17 (≠ V18), S18 (= S19), H19 (= H20), A20 (= A21), L21 (≠ N22), L21 (≠ N22), N22 (= N23), K25 (≠ R26), R26 (= R27), R27 (= R28), F28 (= F29), L29 (= L30), P30 (= P31), N31 (= N32), H33 (≠ Q34), H35 (= H36), R36 (= R37), R49 (= R50), A52 (= A53), K53 (= K54), M55 (= M56), R56 (= R57), V57 (≠ I58), K60 (= K61), K76 (= K77)

8rd8WD Small ribosomal subunit protein uS5 (see paper)
83% identity, 97% coverage: 2:77/78 of query aligns to 1:76/76 of 8rd8WD

query
sites
8rd8WD

S
|
S

R
|
R

V

V

C

C

Q

Q

V

V

T

T

G

G

K
|
K

G
x
R

P

P

V
x
M

T
x
V

G
|
G

N
|
N

N

N

V

V

S
|
S

H
|
H

A
|
A

N
|
N

N
|
N

K
|
K

T

T

R
|
R

R
|
R

R
|
R

F
|
F

L
|
L

P
|
P

N
|
N

L

L

Q
x
H

H

H

H
|
H

R
|
R

F

F

W

W

V

V

E

E

S

S

E

E

K

N

R

R

F

F

V

V

R
|
R

L

L

R
|
R

V

I

S

S

A
x
T

K
|
K

G

G

M

M

R
|
R

I

I

I

I

D

D

K
|
K

R
x
L

G

G

I

I

D

D

V

K

V

V

L

L

S

V

E

D

L

L

R

R

A

A

R

Q

G

G

E

Q

K

K

binding : S1 (= S2), R2 (= R3), K9 (= K10), R10 (≠ G11), M12 (≠ V13), V13 (≠ T14), G14 (= G15), N15 (= N16), S18 (= S19), H19 (= H20), A20 (= A21), N21 (= N22), N22 (= N23), K23 (= K24), R25 (= R26), R26 (= R27), R27 (= R28), F28 (= F29), L29 (= L30), P30 (= P31), N31 (= N32), H33 (≠ Q34), H35 (= H36), R36 (= R37), R47 (= R48), R49 (= R50), T52 (≠ A53), K53 (= K54), R56 (= R57), K60 (= K61), L61 (≠ R62)

7uvxW 50S ribosomal protein L28 (see paper)
76% identity, 100% coverage: 1:78/78 of query aligns to 1:78/78 of 7uvxW

query
sites
7uvxW

M
|
M

S
|
S

R
x
K

V

V

C

C

Q

Q

V

V

T

T

G

G

K
|
K

G

R

P

P

V

V

T

V

G

G

N
|
N

N

N

V

V

S
|
S

H
|
H

A
|
A

N
|
N

N
|
N

K

K

T

T

R
x
K

R
|
R

R

R

F
|
F

L
x
E

P
|
P

N
|
N

L

L

Q
x
H

H

H

H
|
H

R
|
R

F

F

W

W

V

L

E

E

S

S

E

E

K

K

R

R

F

F

V

V

R

R

L

L

R
|
R

V

L

S

T

A
x
T

K
|
K

G

G

M

M

R
|
R

I

I

I

I

D

D

K
|
K

R

L

G

G

I

I

D

E

V

K

V

V

L

V

S

A

E

D

L

L

R

R

A

A

R

Q

G

G

E

Q

K

K

V

I

binding Streptothricin F: H20 (= H20), N22 (= N22)
binding : M1 (= M1), S2 (= S2), K3 (≠ R3), K10 (= K10), N16 (= N16), S19 (= S19), A21 (= A21), N22 (= N22), N23 (= N23), K26 (≠ R26), R27 (= R27), F29 (= F29), E30 (≠ L30), P31 (= P31), N32 (= N32), H34 (≠ Q34), H36 (= H36), R37 (= R37), R50 (= R50), T53 (≠ A53), K54 (= K54), R57 (= R57), K61 (= K61)

7uvyW 50S ribosomal protein L2 (see paper)
75% identity, 99% coverage: 2:78/78 of query aligns to 1:77/77 of 7uvyW

query
sites
7uvyW

S
|
S

R
x
K

V

V

C

C

Q

Q

V

V

T

T

G

G

K
|
K

G

R

P

P

V
|
V

T
x
V

G

G

N
|
N

N
|
N

V
|
V

S
|
S

H
|
H

A
|
A

N
|
N

N
|
N

K
|
K

T

T

R
x
K

R
|
R

R
|
R

F
|
F

L
x
E

P
|
P

N
|
N

L

L

Q
x
H

H

H

H
|
H

R
|
R

F

F

W

W

V

L

E

E

S

S

E

E

K

K

R

R

F

F

V

V

R

R

L

L

R
|
R

V

L

S

T

A
x
T

K
|
K

G

G

M
|
M

R
|
R

I

I

I

I

D

D

K
|
K

R

L

G

G

I

I

D

E

V

K

V

V

L

V

S

A

E

D

L

L

R

R

A

A

R

Q

G

G

E

Q

K

K

V

I

binding Streptothricin D: N16 (= N17), V17 (= V18), S18 (= S19), H19 (= H20), N21 (= N22)
binding : S1 (= S2), K2 (≠ R3), K9 (= K10), V12 (= V13), V13 (≠ T14), N15 (= N16), S18 (= S19), A20 (= A21), N21 (= N22), N22 (= N23), K23 (= K24), K25 (≠ R26), R26 (= R27), R27 (= R28), F28 (= F29), E29 (≠ L30), P30 (= P31), N31 (= N32), H33 (≠ Q34), H35 (= H36), R36 (= R37), R49 (= R50), T52 (≠ A53), K53 (= K54), M55 (= M56), R56 (= R57), K60 (= K61)

7jilX 50S ribosomal protein L15 (see paper)
47% identity, 95% coverage: 2:75/78 of query aligns to 1:74/76 of 7jilX

query
sites
7jilX

S
|
S

R
|
R

V

V

C

C

Q

D

V

L

T

T

G

G

K
|
K

G
x
R

P

A

V
x
M

T
x
V

G

G

N
|
N

N

N

V
|
V

S
|
S

H

H

A
|
A

N
x
M

N
|
N

K
|
K

T

T

R
x
K

R
|
R

R
x
K

F
|
F

L
x
S

P
x
V

N
|
N

L

L

Q

V

H
x
K

H
x
K

R
|
R

F

F

W

Y

V

L

E

P

S

E

E

E

K

D

R

R

F

W

V

I

R

T

L

L

R
|
R

V

V

S

Q

A
x
R

K

S

G

T

M

I

R
x
K

I

T

I

I

D

N

K
|
K

R

N

G

G

I

I

D

T

V

A

V

V

L

L

S

K

E

K

L

A

R

Q

A

S

R

A

G

G

binding : S1 (= S2), R2 (= R3), K9 (= K10), R10 (≠ G11), M12 (≠ V13), V13 (≠ T14), N15 (= N16), V17 (= V18), S18 (= S19), A20 (= A21), M21 (≠ N22), N22 (= N23), K23 (= K24), K25 (≠ R26), R26 (= R27), K27 (≠ R28), F28 (= F29), S29 (≠ L30), V30 (≠ P31), N31 (= N32), K34 (≠ H35), K35 (≠ H36), R36 (= R37), R49 (= R50), R52 (≠ A53), K56 (≠ R57), K60 (= K61)

5mmiY Structure of the large subunit of the chloroplast ribosome (see paper)
49% identity, 83% coverage: 3:67/78 of query aligns to 2:66/77 of 5mmiY

query
sites
5mmiY

R
|
R

V

I

C

C

Q

P

V

F

T

T

G

G

K
|
K

G

K

P

S

V
x
N

T
x
K

G
x
A

N
|
N

N
x
R

V

V

S
|
S

H

H

A
x
S

N
|
N

N
x
H

K

K

T

T

R
x
K

R
|
R

R

L

F
x
Q

L
x
F

P
x
V

N
|
N

L

L

Q

Q

H
x
Y

H
x
K

R
|
R

F

V

W

W

V

W

E

E

S

A

E

G

K

K

R

R

F

F

V

V

R
x
K

L

L

R
|
R

V

L

S

S

A
x
T

K
|
K

G

A

M

L

R
x
K

I

T

I

I

D

E

K
|
K

R
x
N

G

G

I

L

D

D

V

A

V

V

binding : R2 (= R3), K9 (= K10), N12 (≠ V13), K13 (≠ T14), A14 (≠ G15), N15 (= N16), R16 (≠ N17), S18 (= S19), S20 (≠ A21), N21 (= N22), H22 (≠ N23), K25 (≠ R26), R26 (= R27), Q28 (≠ F29), F29 (≠ L30), V30 (≠ P31), N31 (= N32), Y34 (≠ H35), K35 (≠ H36), R36 (= R37), K47 (≠ R48), R49 (= R50), T52 (≠ A53), K53 (= K54), K56 (≠ R57), K60 (= K61), N61 (≠ R62)

Sites not aligning to the query:

binding : 1, 68, 75

4v61BY Ribosomal Protein S8 (see paper)
46% identity, 83% coverage: 3:67/78 of query aligns to 1:65/76 of 4v61BY

query
sites
4v61BY

R
|
R

V

I

C

C

Q

P

V
x
F

T
|
T

G

G

K

K

G

K

P

S

V

N

T

R

G
x
A

N
|
N

N
x
K

V
|
V

S

S

H
|
H

A
x
S

N
|
N

N
x
H

K

K

T

T

R
x
K

R

K

R
x
L

F

Q

L

F

P
x
V

N
|
N

L
|
L

Q
|
Q

H

Y

H

K

R
|
R

F

I

W
|
W

V

W

E
|
E

S

A

E

G

K

K

R
|
R

F
x
Y

V

V

R

K

L

L

R

R

V

L

S

S

A

T

K
|
K

G

A

M

I

R

K

I

T

I

I

D

E

K

K

R

N

G

G

I

L

D

D

V

A

V

V

binding : R1 (= R3), F5 (≠ V7), T6 (= T8), A13 (≠ G15), N14 (= N16), K15 (≠ N17), V16 (= V18), H18 (= H20), S19 (≠ A21), N20 (= N22), H21 (≠ N23), K24 (≠ R26), L26 (≠ R28), V29 (≠ P31), N30 (= N32), L31 (= L33), Q32 (= Q34), R35 (= R37), W37 (= W39), E39 (= E41), R43 (= R45), Y44 (≠ F46), K52 (= K54)

Sites not aligning to the query:

binding : 68, 69, 71, 72, 73, 76

6yweS structure of the mitoribosome from Neurospora crassa in the P/E tRNA bound state (see paper)
52% identity, 63% coverage: 15:63/78 of query aligns to 63:112/179 of 6yweS

query
sites
6yweS

G

G

N
|
N

N

N

V

V

S
|
S

H

K

A
x
R

N
|
N

N
x
E

-
x
I

K
|
K

T

T

R
|
R

R
|
R

R

K

F
x
W

L
x
R

P
|
P

N
|
N

L

V

Q
|
Q

H
x
Q

H
x
K

R
|
R

F

L

W

W

V

S

E

K

S

S

E

L

K

G

R

V

F

F

V

V

R

R

L

T

R

R

V

V

S

T

A
x
T

K
x
R

G

V

M

L

R
|
R

I
x
T

I

I

D

D

K
|
K

R

V

G

G

binding : N64 (= N16), S67 (= S19), R69 (≠ A21), N70 (= N22), E71 (≠ N23), I72 (vs. gap), K73 (= K24), R75 (= R26), R76 (= R27), W78 (≠ F29), R79 (≠ L30), P80 (= P31), N81 (= N32), Q83 (= Q34), Q84 (≠ H35), K85 (≠ H36), R86 (= R37), T102 (≠ A53), R103 (≠ K54), R106 (= R57), T107 (≠ I58), K110 (= K61)

Sites not aligning to the query:

binding magnesium ion: 47, 49
binding : 2, 5, 17, 19, 45, 47, 48, 49, 61, 62, 121, 122, 123, 124, 125, 157

8fmwAZ 30S ribosomal protein S10 (see paper)
34% identity, 88% coverage: 2:70/78 of query aligns to 1:82/91 of 8fmwAZ

query
sites
8fmwAZ

S
x
A

R
|
R

V

K

C

C

Q

E

V

I

T

T

G

G

K
|
K

G

K

P
x
T

V
x
M

T
x
F

G

G

N
|
N

N

N

V

V

-

P

-
x
R

-
x
K

-

G

-

L

-

A

-
x
K

-

K

-
x
K

-

G

-

G

-
x
A

-

G

S
x
Q

H
|
H

A
x
I

N
x
G

N

V

K
|
K

T
|
T

R
x
K

R
|
R

R

T

F
|
F

L
x
K

P
x
V

N
|
N

L

L

Q
x
I

H

N

H

K

R
x
K

F

F

W

F

V

I

E

P

S

N

E

L

K

G

R
|
R

F
x
S

V

V

R

S

L

I

R

K

V

V

S

S

A
|
A

K

N

G

A

M

L

R
|
R

I

S

I

I

D

S

K
|
K

R

I

G

G

I

L

D

D

V

A

V

F

L

L

S
x
K

E
x
K

binding : A1 (≠ S2), R2 (= R3), K9 (= K10), T11 (≠ P12), M12 (≠ V13), F13 (≠ T14), N15 (= N16), R19 (vs. gap), K20 (vs. gap), K24 (vs. gap), K26 (vs. gap), A29 (vs. gap), Q31 (≠ S19), H32 (= H20), I33 (≠ A21), G34 (≠ N22), K36 (= K24), T37 (= T25), K38 (≠ R26), R39 (= R27), F41 (= F29), K42 (≠ L30), V43 (≠ P31), N44 (= N32), I46 (≠ Q34), K49 (≠ R37), R57 (= R45), S58 (≠ F46), A65 (= A53), R69 (= R57), K73 (= K61), K81 (≠ S69), K82 (≠ E70)

Sites not aligning to the query:

binding : 86, 88

5mrcS of the yeast mitochondrial ribosome - Class A (see paper)
35% identity, 76% coverage: 15:73/78 of query aligns to 61:120/185 of 5mrcS

query
sites
5mrcS

G

G

N
|
N

N
|
N

V
x
I

S
|
S

H

E

A
x
S

N
x
K

N

A

K
|
K

T

T

R
|
R

R
x
K

R

K

F
x
W

L
|
L

P
|
P

N
|
N

L

V

Q

V

H

K

H
x
K

R

G

F

L

W

W

V

S

E

E

S

T

E

L

K

N

R

R

F

K

V
x
I

R
x
S

L
x
I

R

K

V

M

S

T

A
|
A

K
|
K

G

V

M
x
L

R
x
K

I
x
T

I

I

D

S

K
|
K

R

E

-

G

G

G

I

I

D

D

V

N

V

Y

L

L

S

T

E
x
K

L

E

R
x
K

A
x
S

binding : N62 (= N16), N63 (= N17), I64 (≠ V18), S65 (= S19), S67 (≠ A21), K68 (≠ N22), K70 (= K24), R72 (= R26), K73 (≠ R27), W75 (≠ F29), L76 (= L30), P77 (= P31), N78 (= N32), K82 (≠ H36), I93 (≠ V47), S94 (≠ R48), I95 (≠ L49), A99 (= A53), K100 (= K54), L102 (≠ M56), K103 (≠ R57), T104 (≠ I58), K107 (= K61), K117 (≠ E70), K119 (≠ R72), S120 (≠ A73)

Sites not aligning to the query:

binding : 1, 2, 3, 4, 8, 10, 11, 12, 13, 25, 26, 34, 43, 44, 45, 46, 47, 50, 56, 59, 60, 121, 122, 124, 133, 136, 140, 148, 149, 151, 152, 153, 172, 175, 177

7f0dX Cryo-em structure of mycobacterium tuberculosis 50s ribosome subunit bound with clarithromycin (see paper)
50% identity, 44% coverage: 1:34/78 of query aligns to 1:34/63 of 7f0dX

query
sites
7f0dX

M
|
M

S
x
A

R

A

V

V

C

C

Q

D

V

I

T

C

G

G

K
|
K

G

G

P
|
P

V

G

T
x
F

G

G

N
x
K

N

S

V
|
V

S
|
S

H
|
H

A
x
S

N
x
H

N
x
R

K
x
R

T
|
T

R

S

R
|
R

R

R

F
x
W

L
x
D

P
|
P

N
|
N

L

I

Q
|
Q

binding : M1 (= M1), A2 (≠ S2), K10 (= K10), P12 (= P12), F14 (≠ T14), K16 (≠ N16), V18 (= V18), S19 (= S19), H20 (= H20), S21 (≠ A21), H22 (≠ N22), R23 (≠ N23), R24 (≠ K24), T25 (= T25), R27 (= R27), W29 (≠ F29), D30 (≠ L30), P31 (= P31), N32 (= N32), Q34 (= Q34)

Sites not aligning to the query:

binding : 35, 36, 37, 45, 46, 47, 53, 57, 58

Query Sequence

>OH686_13630
MSRVCQVTGKGPVTGNNVSHANNKTRRRFLPNLQHHRFWVESEKRFVRLRVSAKGMRIID
KRGIDVVLSELRARGEKV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory