SitesBLAST

binding : M1 (= M1), K2 (= K2), A5 (= A5), S6 (= S6), V7 (= V7), K8 (= K8), K15 (= K15), R18 (≠ K18), R19 (= R19), K20 (≠ D20), P31 (= P31), R32 (≠ K32), K34 (= K34), R36 (= R36), Q37 (= Q37)

4v61B6 Ribosomal Protein S8 (see paper)
71% identity, 100% coverage: 1:38/38 of query aligns to 1:38/38 of 4v61B6

query
sites
4v61B6

M
|
M

K

K

V
|
V

R
|
R

A
x
S

S
|
S

V

V

K
|
K

K

K

L
x
M

C

C

R

E

N

F

C

C

K
|
K

I

T

V

V

K

K

R
|
R

D

R

G

G

V

R

I

V

R

Y

V

V

I

I

C

C

S

S

A

S

E

N

P
|
P

K
|
K

H

H

K

K

Q

Q

R
|
R

Q
|
Q

G

G

binding : M1 (= M1), V3 (= V3), R4 (= R4), S5 (≠ A5), S6 (= S6), K8 (= K8), M10 (≠ L10), K15 (= K15), R19 (= R19), P31 (= P31), K32 (= K32), R36 (= R36), Q37 (= Q37)

7nhk8 50S ribosomal protein L36 (see paper)
63% identity, 100% coverage: 1:38/38 of query aligns to 1:38/38 of 7nhk8

query
sites
7nhk8

M
|
M

K
|
K

V

V

R
|
R

A
x
P

S
|
S

V
|
V

K
|
K

K

P

L
x
M

C

C

R

E

N

H

C

C

K

K

I

V

V

I

K
x
R

R
|
R

D
x
K

G

G

V
x
R

I

V

R

M

V

V

I

I

C
|
C

S

P

A

A

E

N

P

P

K
|
K

H
|
H

K

K

Q

Q

R
|
R

Q
|
Q

G
|
G

binding zinc ion: C27 (= C27), H33 (= H33)
binding : M1 (= M1), K2 (= K2), R4 (= R4), P5 (≠ A5), S6 (= S6), V7 (= V7), K8 (= K8), M10 (≠ L10), R18 (≠ K18), R19 (= R19), K20 (≠ D20), R22 (≠ V22), K32 (= K32), R36 (= R36), Q37 (= Q37), G38 (= G38)

7ood2 Mycoplasma pneumoniae 50s subunit of ribosomes in chloramphenicol- treated cells (see paper)
66% identity, 100% coverage: 1:38/38 of query aligns to 1:37/37 of 7ood2

query
sites
7ood2

M
|
M

K
|
K

V
|
V

R
|
R

A
|
A

S
|
S

V
|
V

K

K

K

P

L
x
I

C
|
C

R

K

N

D

C

C

K

K

I

I

V

I

K

K

R
|
R

D

H

G

Q

V

I

I

V

R

R

V

V

I

I

C
|
C

S

K

A

T

E

Q

P

-

K
|
K

H
|
H

K
|
K

Q

Q

R
|
R

Q
|
Q

G

G

binding zinc ion: C11 (= C11), C27 (= C27), H32 (= H33)
binding : M1 (= M1), K2 (= K2), V3 (= V3), R4 (= R4), A5 (= A5), S6 (= S6), V7 (= V7), I10 (≠ L10), R19 (= R19), K31 (= K32), K33 (= K34), R35 (= R36), Q36 (= Q37)

3cf54 Thiopeptide antibiotic thiostrepton bound to the large ribosomal subunit of deinococcus radiodurans (see paper)
68% identity, 100% coverage: 1:38/38 of query aligns to 1:37/37 of 3cf54

query
sites
3cf54

M
|
M

K
|
K

V

V

R
|
R

A
x
S

S
|
S

V
|
V

K
|
K

L

M

C

C

R

D

N

N

C

C

K
|
K

I
x
V

V

V

K
x
R

R
|
R

D
x
H

G

G

V

R

I
x
V

R

L

V

V

I

I

C

C

S

S

A

-

E

N

P
x
V

K
|
K

H

H

K

K

Q

Q

R
|
R

Q
|
Q

G

G

binding : M1 (= M1), K2 (= K2), R4 (= R4), S5 (≠ A5), S6 (= S6), V7 (= V7), K8 (= K8), K9 (= K9), K15 (= K15), V16 (≠ I16), R18 (≠ K18), R19 (= R19), H20 (≠ D20), V23 (≠ I23), V30 (≠ P31), K31 (= K32), R35 (= R36), Q36 (= Q37)

2zjp4 Thiopeptide antibiotic nosiheptide bound to the large ribosomal subunit of deinococcus radiodurans (see paper)
68% identity, 100% coverage: 1:38/38 of query aligns to 1:37/37 of 2zjp4

query
sites
2zjp4

M
|
M

K
|
K

V

V

R
|
R

A
x
S

S
|
S

V
|
V

K
|
K

L
x
M

C
|
C

R

D

N

N

C

C

K
|
K

I

V

V
|
V

K
x
R

R
|
R

D
x
H

G

G

V

R

I

V

R

L

V

V

I

I

C

C

S

S

A

-

E

N

P
x
V

K
|
K

H
|
H

K

K

Q

Q

R
|
R

Q
|
Q

G

G

binding zinc ion: M10 (≠ L10), C11 (= C11), H32 (= H33)
binding : M1 (= M1), K2 (= K2), R4 (= R4), S5 (≠ A5), S6 (= S6), V7 (= V7), K8 (= K8), K9 (= K9), K15 (= K15), V17 (= V17), R18 (≠ K18), R19 (= R19), H20 (≠ D20), V30 (≠ P31), K31 (= K32), R35 (= R36), Q36 (= Q37)

1vy4B9 50S ribosomal protein L36 (see paper)
63% identity, 100% coverage: 1:38/38 of query aligns to 1:37/37 of 1vy4B9

query
sites
1vy4B9

M
|
M

K
|
K

V
|
V

R
|
R

A
|
A

S
|
S

V
|
V

K
|
K

K
x
R

L

I

C
|
C

R

D

N

K

C
|
C

K
|
K

I
x
V

V

I

K
x
R

R
|
R

D
x
H

G

G

V
x
R

I

V

R

Y

V

V

I

I

C
|
C

S

E

A

-

E

N

P

P

K
|
K

H
|
H

K
|
K

Q

Q

R
|
R

Q
|
Q

G
|
G

binding zinc ion: C11 (= C11), C14 (= C14), C27 (= C27), H32 (= H33)
binding : M1 (= M1), K2 (= K2), V3 (= V3), R4 (= R4), A5 (= A5), S6 (= S6), V7 (= V7), K8 (= K8), R9 (≠ K9), K15 (= K15), V16 (≠ I16), R18 (≠ K18), R19 (= R19), H20 (≠ D20), R22 (≠ V22), K31 (= K32), K33 (= K34), R35 (= R36), Q36 (= Q37), G37 (= G38)

1dfeA Nmr structure of ribosomal protein l36 from thermus thermophilus (see paper)
63% identity, 100% coverage: 1:38/38 of query aligns to 1:37/37 of 1dfeA

query
sites
1dfeA

M

M

K

K

V

V

R

R

A

A

S

S

V

V

K

K

K

R

L

I

C
|
C

R

D

N

K

C
|
C

K

K

I

V

V

I

K

R

R

R

D

H

G

G

V

R

I

V

R

Y

V

V

I

I

C
|
C

S

E

A

-

E

N

P

P

K

K

H
|
H

K

K

Q

Q

R

R

Q

Q

G

G

binding zinc ion: C11 (= C11), C14 (= C14), C27 (= C27), H32 (= H33)

P80256 Large ribosomal subunit protein bL36; 50S ribosomal protein L36; Ribosomal protein B from Thermus thermophilus (see paper)
63% identity, 100% coverage: 1:38/38 of query aligns to 1:37/37 of P80256

query
sites
P80256

M

M

K

K

V

V

R

R

A

A

S

S

V

V

K

K

K

R

L

I

C
|
C

R

D

N

K

C
|
C

K

K

I

V

V

I

K

R

R

R

D

H

G

G

V

R

I

V

R

Y

V

V

I

I

C
|
C

S

E

A

-

E

N

P

P

K

K

H
|
H

K

K

Q

Q

R

R

Q

Q

G

G

C11 (= C11) binding Zn(2+)
C14 (= C14) binding Zn(2+)
C27 (= C27) binding Zn(2+)
H32 (= H33) binding Zn(2+)

5li08 50S ribosomal protein L36 (see paper)
63% identity, 100% coverage: 1:38/38 of query aligns to 1:37/37 of 5li08

query
sites
5li08

M
|
M

K
|
K

V

V

R
|
R

A
x
P

S
|
S

V
|
V

K
|
K

K

P

L
x
I

C

C

R

E

N

K

C

C

K

K

I

V

V

I

K
|
K

R
|
R

D

K

G

G

V

K

I

V

R

M

V

V

I

I

C
|
C

S

E

A

-

E

N

P

P

K
|
K

H
|
H

K
|
K

Q

Q

R
|
R

Q
|
Q

G

G

binding zinc ion: C27 (= C27), H32 (= H33)
binding : M1 (= M1), K2 (= K2), R4 (= R4), P5 (≠ A5), S6 (= S6), V7 (= V7), K8 (= K8), I10 (≠ L10), K18 (= K18), R19 (= R19), K31 (= K32), K33 (= K34), R35 (= R36), Q36 (= Q37)

3j6b0 of the yeast mitochondrial large ribosomal subunit (see paper)
53% identity, 100% coverage: 1:38/38 of query aligns to 1:38/38 of 3j6b0

query
sites
3j6b0

M
x
F

K
|
K

V
|
V

R
|
R

A
x
T

S
|
S

V
|
V

K

K

L
x
F

C
|
C

R

S

N

D

C

C

K
x
Y

I
x
L

V
|
V

K
x
R

R
|
R

D
x
K

G
|
G

V
x
R

I
x
V

R
x
Y

V

I

I

Y

C

C

S
x
K

A

S

E

N

P
x
K

K
|
K

H
|
H

K
|
K

Q

Q

R
|
R

Q
|
Q

G

G

binding zinc ion: C11 (= C11), H33 (= H33)
binding : F1 (≠ M1), K2 (= K2), V3 (= V3), R4 (= R4), T5 (≠ A5), S6 (= S6), V7 (= V7), F10 (≠ L10), Y15 (≠ K15), L16 (≠ I16), V17 (= V17), R18 (≠ K18), R19 (= R19), K20 (≠ D20), G21 (= G21), R22 (≠ V22), V23 (≠ I23), Y24 (≠ R24), K28 (≠ S28), K31 (≠ P31), K32 (= K32), K34 (= K34), R36 (= R36), Q37 (= Q37)

4v8hB9 structure of HPF bound to the 70S ribosome. (see paper)
62% identity, 97% coverage: 2:38/38 of query aligns to 1:36/36 of 4v8hB9

query
sites
4v8hB9

K
|
K

V

V

R
|
R

A
|
A

S
|
S

V
|
V

K
|
K

K
x
R

L

I

C
|
C

R

D

N

K

C
|
C

K
|
K

I
x
V

V

I

K
x
R

R
|
R

D
x
H

G

G

V
x
R

I

V

R

Y

V

V

I

I

C
|
C

S

E

A

-

E

N

P

P

K
|
K

H
|
H

K
|
K

Q
|
Q

R
|
R

Q
|
Q

G
|
G

binding magnesium ion: K1 (= K2), Q33 (= Q35)
binding zinc ion: C10 (= C11), C13 (= C14), C26 (= C27), H31 (= H33)
binding : K1 (= K2), R3 (= R4), A4 (= A5), S5 (= S6), V6 (= V7), K7 (= K8), R8 (≠ K9), K14 (= K15), V15 (≠ I16), R17 (≠ K18), R18 (= R19), H19 (≠ D20), R21 (≠ V22), K30 (= K32), K32 (= K34), R34 (= R36), Q35 (= Q37), G36 (= G38)

6ywx0 structure of the mitoribosome from Neurospora crassa with tRNA bound to the E-site (see paper)
48% identity, 100% coverage: 1:38/38 of query aligns to 1:44/46 of 6ywx0

query
sites
6ywx0

M
|
M

K
|
K

V
|
V

R

H

A
x
S

S
x
A

V
x
I

K
|
K

K

K

L

R

C
|
C

R

E

N

H

C

C

K
|
K

I

V

V
|
V

K
x
R

R
|
R

-
x
K

-

A

-

N

-

K

-

R

-

Q

D
x
N

G

G

V
x
Y

I

L

R
x
Y

V

I

I

I

C
|
C

S

P

A

A

E

N

P

P

K
x
R

H
|
H

K
|
K

Q

Q

R
|
R

Q
|
Q

G
|
G

binding magnesium ion: K20 (vs. gap), Y30 (≠ R24)
binding zinc ion: C11 (= C11), C33 (= C27), H39 (= H33)
binding : M1 (= M1), K2 (= K2), V3 (= V3), S5 (≠ A5), A6 (≠ S6), I7 (≠ V7), K8 (= K8), K15 (= K15), V17 (= V17), R18 (≠ K18), R19 (= R19), K20 (vs. gap), N26 (≠ D20), Y28 (≠ V22), Y30 (≠ R24), R38 (≠ K32), K40 (= K34), R42 (= R36), Q43 (= Q37), G44 (= G38)

Sites not aligning to the query:

binding : 45, 46

6ywe0 structure of the mitoribosome from Neurospora crassa in the P/E tRNA bound state (see paper)
48% identity, 100% coverage: 1:38/38 of query aligns to 1:44/46 of 6ywe0

query
sites
6ywe0

M
|
M

K
|
K

V
|
V

R
x
H

A
x
S

S
x
A

V
x
I

K
|
K

K

K

L

R

C
|
C

R

E

N

H

C

C

K
|
K

I

V

V
|
V

K
x
R

R
|
R

-
x
K

-

A

-

N

-

K

-

R

-

Q

D
x
N

G

G

V
x
Y

I

L

R
x
Y

V

I

I

I

C
|
C

S

P

A

A

E

N

P
|
P

K
x
R

H
|
H

K
|
K

Q

Q

R
|
R

Q
|
Q

G
|
G

binding zinc ion: C11 (= C11), C33 (= C27), H39 (= H33)
binding : M1 (= M1), K2 (= K2), V3 (= V3), H4 (≠ R4), S5 (≠ A5), A6 (≠ S6), I7 (≠ V7), K8 (= K8), K15 (= K15), V17 (= V17), R18 (≠ K18), R19 (= R19), K20 (vs. gap), N26 (≠ D20), Y28 (≠ V22), Y30 (≠ R24), P37 (= P31), R38 (≠ K32), K40 (= K34), R42 (= R36), Q43 (= Q37), G44 (= G38)

Sites not aligning to the query:

binding : 45, 46

7as8j Bacillus subtilis ribosome quality control complex state b. Ribosomal 50s subunit with p-tRNA, rqch, and rqcp/yabo (see paper)
58% identity, 100% coverage: 1:38/38 of query aligns to 1:37/37 of 7as8j

query
sites
7as8j

M
|
M

K
|
K

V

V

R
|
R

A
x
P

S
|
S

V
|
V

K
|
K

K

P

L

I

C

C

R

E

N

K

C

C

K

K

I

V

V

I

K
x
R

R
|
R

D
x
K

G

G

V

K

I

V

R

M

V

V

I

I

C

C

S

E

A

-

E

N

P
|
P

K
|
K

H

H

K

K

Q

Q

R
x
K

Q
|
Q

G
|
G

binding : M1 (= M1), K2 (= K2), R4 (= R4), P5 (≠ A5), S6 (= S6), V7 (= V7), K8 (= K8), R18 (≠ K18), R19 (= R19), K20 (≠ D20), P30 (= P31), K31 (= K32), K35 (≠ R36), Q36 (= Q37), G37 (= G38)

Query Sequence

>OHPLBJKB_00404
MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory