SitesBLAST
Comparing PP_2784 FitnessBrowser__Putida:PP_2784 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3sjuA Hedamycin polyketide ketoreductase bound to NADPH (see paper)
35% identity, 97% coverage: 5:251/254 of query aligns to 1:253/255 of 3sjuA
- active site: G11 (= G15), S138 (= S136), Y151 (= Y149), K155 (= K153), Y196 (≠ Q194)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G11), S10 (≠ N14), A31 (≠ L35), R32 (= R37), D33 (= D38), C56 (≠ A60), D57 (≠ N61), V58 (≠ I62), S84 (= S88), A85 (= A89), G86 (= G90), I136 (≠ M134), Y151 (= Y149), K155 (= K153), P181 (= P179), G182 (≠ A180), Y183 (≠ W181), V184 (≠ I182), T186 (= T184), M188 (= M186)
1xr3A Actinorhodin polyketide ketoreductase with NADP and the inhibitor isoniazid bound (see paper)
35% identity, 96% coverage: 8:251/254 of query aligns to 5:254/256 of 1xr3A
- active site: G12 (= G15), N109 (= N110), S139 (= S136), Y152 (= Y149), K156 (= K153), Y197 (≠ Q194)
- binding 4-(diazenylcarbonyl)pyridine: T140 (≠ I137), G141 (≠ L138), V146 (≠ K143)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (≠ A13), S11 (≠ N14), G12 (= G15), I13 (= I16), A32 (≠ L35), R33 (= R37), G34 (≠ D38), C57 (≠ A60), D58 (≠ N61), V59 (≠ I62), N85 (≠ S88), A86 (= A89), G87 (= G90), S139 (= S136), Y152 (= Y149), K156 (= K153), G183 (≠ A180), V185 (≠ I182), T187 (= T184), P188 (= P185)
2rh4B Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
35% identity, 96% coverage: 8:251/254 of query aligns to 17:266/268 of 2rh4B
- active site: G24 (= G15), N121 (= N110), S151 (= S136), Y164 (= Y149), K168 (= K153), Y209 (≠ Q194)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G11), T22 (≠ A13), S23 (≠ N14), I25 (= I16), A44 (≠ L35), R45 (= R37), G46 (≠ D38), C69 (≠ A60), D70 (≠ N61), V71 (≠ I62), N97 (≠ S88), S151 (= S136), Y164 (= Y149), K168 (= K153), G195 (≠ A180), V197 (≠ I182), T199 (= T184), M201 (= M186)
2rh4A Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
35% identity, 96% coverage: 8:251/254 of query aligns to 6:255/257 of 2rh4A
- active site: G13 (= G15), N110 (= N110), S140 (= S136), Y153 (= Y149), K157 (= K153), Y198 (≠ Q194)
- binding 3-methyl-1,6,8-trihydroxyanthraquinone: T141 (≠ I137), Q145 (≠ A141), V147 (≠ K143), Y153 (= Y149), F185 (≠ W181), L254 (≠ A250)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G11), T11 (≠ A13), S12 (≠ N14), G13 (= G15), I14 (= I16), A33 (≠ L35), R34 (= R37), G35 (≠ D38), C58 (≠ A60), D59 (≠ N61), V60 (≠ I62), N86 (≠ S88), G88 (= G90), S140 (= S136), Y153 (= Y149), K157 (= K153), P183 (= P179), G184 (≠ A180), V186 (≠ I182), T188 (= T184), M190 (= M186)
P16544 Putative ketoacyl reductase; EC 1.3.1.- from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
35% identity, 96% coverage: 8:251/254 of query aligns to 10:259/261 of P16544
- 11:39 (vs. 9:38, 27% identical) binding
- D63 (≠ N61) binding
- K161 (= K153) binding
1w4zA Structure of actinorhodin polyketide (actiii) reductase (see paper)
35% identity, 96% coverage: 8:251/254 of query aligns to 8:257/259 of 1w4zA
- active site: G15 (= G15), N112 (= N110), S142 (= S136), Y155 (= Y149), K159 (= K153), Y200 (≠ Q194)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), T13 (≠ A13), S14 (≠ N14), G15 (= G15), I16 (= I16), R36 (= R37), G37 (≠ D38), D61 (≠ N61), V62 (≠ I62), N88 (≠ S88), G90 (= G90), S142 (= S136), Y155 (= Y149), K159 (= K153), P185 (= P179), G186 (≠ A180), V188 (≠ I182), T190 (= T184)
5b4tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and a substrate d-3- hydroxybutyrate (see paper)
31% identity, 98% coverage: 1:249/254 of query aligns to 1:256/260 of 5b4tA
- active site: G15 (= G15), N114 (= N110), S142 (= S136), Y155 (= Y149), K159 (= K153), I200 (≠ Q194)
- binding (3R)-3-hydroxybutanoic acid: Q94 (≠ C92), S142 (= S136), H144 (≠ L138), K152 (≠ N146), Y155 (= Y149), W187 (= W181), Q196 (≠ E190)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), T13 (≠ A13), G15 (= G15), I16 (= I16), F36 (vs. gap), D63 (≠ N61), L64 (≠ I62), N90 (≠ S88), G92 (= G90), L113 (≠ I109), I140 (≠ M134), Y155 (= Y149), K159 (= K153), P185 (= P179), G186 (≠ A180), W187 (= W181), V188 (≠ I182), T190 (= T184), L192 (≠ M186), V193 (≠ L187)
3w8dA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and an inhibitor methylmalonate
31% identity, 98% coverage: 1:249/254 of query aligns to 1:256/260 of 3w8dA
- active site: G15 (= G15), N114 (= N110), S142 (= S136), Y155 (= Y149), K159 (= K153), I200 (≠ Q194)
- binding methylmalonic acid: Q94 (≠ C92), S142 (= S136), H144 (≠ L138), K152 (≠ N146), Y155 (= Y149), W187 (= W181), Q196 (≠ E190)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), T13 (≠ A13), S14 (≠ N14), G15 (= G15), I16 (= I16), F36 (vs. gap), A62 (= A60), D63 (≠ N61), L64 (≠ I62), N90 (≠ S88), A91 (= A89), G92 (= G90), L113 (≠ I109), S142 (= S136), Y155 (= Y149), K159 (= K153), P185 (= P179), G186 (≠ A180), W187 (= W181), V188 (≠ I182), T190 (= T184), L192 (≠ M186), V193 (≠ L187)
Sites not aligning to the query:
3vdrA Crystal structure of d-3-hydroxybutyrate dehydrogenase, prepared in the presence of the substrate d-3-hydroxybutyrate and NAD(+) (see paper)
31% identity, 98% coverage: 1:249/254 of query aligns to 1:256/260 of 3vdrA
- active site: G15 (= G15), N114 (= N110), S142 (= S136), Y155 (= Y149), K159 (= K153), I200 (≠ Q194)
- binding (3R)-3-hydroxybutanoic acid: Q94 (≠ C92), H144 (≠ L138), K152 (≠ N146), Y155 (= Y149), W187 (= W181), Q196 (≠ E190)
- binding acetoacetic acid: Q94 (≠ C92), H144 (≠ L138), K152 (≠ N146), Y155 (= Y149), W187 (= W181), Q196 (≠ E190)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), T13 (≠ A13), I16 (= I16), F36 (vs. gap), D63 (≠ N61), L64 (≠ I62), N90 (≠ S88), A91 (= A89), G92 (= G90), L113 (≠ I109), K159 (= K153), G186 (≠ A180), V188 (≠ I182), T190 (= T184), L192 (≠ M186), V193 (≠ L187)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G11), T13 (≠ A13), I16 (= I16), F36 (vs. gap), D63 (≠ N61), L64 (≠ I62), N90 (≠ S88), A91 (= A89), G92 (= G90), L113 (≠ I109), S142 (= S136), Y155 (= Y149), K159 (= K153), G186 (≠ A180), V188 (≠ I182), T190 (= T184), L192 (≠ M186), V193 (≠ L187)
Sites not aligning to the query:
3vdqA Crystal structure of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase in complex with NAD(+) and acetate (see paper)
31% identity, 98% coverage: 1:249/254 of query aligns to 1:256/260 of 3vdqA
- active site: G15 (= G15), N114 (= N110), S142 (= S136), Y155 (= Y149), K159 (= K153), I200 (≠ Q194)
- binding acetate ion: Q94 (≠ C92), H144 (≠ L138), K152 (≠ N146), W187 (= W181), L192 (≠ M186), Q196 (≠ E190)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), S14 (≠ N14), I16 (= I16), F36 (vs. gap), D63 (≠ N61), L64 (≠ I62), N90 (≠ S88), A91 (= A89), G92 (= G90), L113 (≠ I109), I140 (≠ M134), S142 (= S136), Y155 (= Y149), K159 (= K153), P185 (= P179), G186 (≠ A180), W187 (= W181), V188 (≠ I182), T190 (= T184), L192 (≠ M186), V193 (≠ L187)
5t5qC Crystal structure of short-chain dehydrogenase/reductase sdr:glucose/ribitol dehydrogenase from brucella melitensis
34% identity, 96% coverage: 5:249/254 of query aligns to 8:241/245 of 5t5qC
- active site: G18 (= G15), S140 (= S136), N150 (= N146), Y153 (= Y149), K157 (= K153)
- binding nicotinamide-adenine-dinucleotide: N16 (≠ A13), G17 (≠ N14), G18 (= G15), I19 (= I16), D38 (= D36), L39 (≠ R37), D63 (≠ N61), A64 (≠ I62), S90 (= S88), I113 (= I109), Y153 (= Y149), K157 (= K153), P182 (= P179), I185 (= I182), T187 (= T184), M189 (= M186)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
33% identity, 97% coverage: 3:249/254 of query aligns to 6:241/244 of 4nbuB
- active site: G18 (= G15), N111 (= N110), S139 (= S136), Q149 (≠ N146), Y152 (= Y149), K156 (= K153)
- binding acetoacetyl-coenzyme a: D93 (≠ E94), K98 (≠ P97), S139 (= S136), N146 (≠ K143), V147 (= V144), Q149 (≠ N146), Y152 (= Y149), F184 (≠ W181), M189 (= M186), K200 (≠ N208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G11), N17 (= N14), G18 (= G15), I19 (= I16), D38 (= D36), F39 (≠ R37), V59 (≠ A60), D60 (≠ N61), V61 (≠ I62), N87 (≠ S88), A88 (= A89), G89 (= G90), I90 (≠ V91), T137 (≠ M134), S139 (= S136), Y152 (= Y149), K156 (= K153), P182 (= P179), F184 (≠ W181), T185 (≠ I182), T187 (= T184), M189 (= M186)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
34% identity, 97% coverage: 3:249/254 of query aligns to 4:244/247 of 4jroC
- active site: G16 (= G15), S142 (= S136), Q152 (≠ N146), Y155 (= Y149), K159 (= K153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), S14 (≠ A13), R15 (≠ N14), G16 (= G15), I17 (= I16), N35 (≠ L34), Y36 (≠ L35), N37 (≠ D36), G38 (vs. gap), S39 (≠ R37), N63 (= N61), V64 (≠ I62), N90 (≠ S88), A91 (= A89), I93 (≠ V91), I113 (= I109), S142 (= S136), Y155 (= Y149), K159 (= K153), P185 (= P179), I188 (= I182), T190 (= T184)
2ztlA Closed conformation of d-3-hydroxybutyrate dehydrogenase complexed with NAD+ and l-3-hydroxybutyrate (see paper)
31% identity, 98% coverage: 1:249/254 of query aligns to 1:256/260 of 2ztlA
- active site: G15 (= G15), N114 (= N110), S142 (= S136), Y155 (= Y149), K159 (= K153), L200 (≠ Q194)
- binding (3s)-3-hydroxybutanoic acid: Q94 (≠ C92), S142 (= S136), H144 (≠ L138), K152 (≠ N146), Y155 (= Y149), Q196 (≠ E190)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), G15 (= G15), I16 (= I16), F36 (≠ W45), L64 (vs. gap), N90 (≠ S88), A91 (= A89), G92 (= G90), L113 (≠ I109), Y155 (= Y149), K159 (= K153), P185 (= P179), W187 (= W181), V188 (≠ I182), T190 (= T184), V193 (≠ L187)
1wmbA Crystal structure of NAD dependent d-3-hydroxybutylate dehydrogenase (see paper)
31% identity, 98% coverage: 1:249/254 of query aligns to 1:256/260 of 1wmbA
Sites not aligning to the query:
5yssB Crystal structure of aminocaproic acid cyclase in complex with NAD (+) (see paper)
31% identity, 96% coverage: 5:249/254 of query aligns to 5:251/255 of 5yssB
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), T13 (≠ A13), S14 (≠ N14), G15 (= G15), I16 (= I16), G35 (≠ L35), F36 (≠ D36), L60 (≠ I62), N86 (≠ S88), G88 (= G90), I89 (≠ V91), A137 (≠ S135), Y151 (= Y149), K155 (= K153), P181 (= P179), G182 (≠ A180), V184 (≠ I182), T186 (= T184)
4b79A The aeropath project and pseudomonas aeruginosa high-throughput crystallographic studies for assessment of potential targets in early stage drug discovery. (see paper)
36% identity, 96% coverage: 7:249/254 of query aligns to 13:232/236 of 4b79A
- active site: G21 (= G15), S133 (= S136), R143 (≠ N146), Y146 (= Y149), K150 (= K153)
- binding nicotinamide-adenine-dinucleotide: G17 (= G11), S19 (≠ A13), S20 (≠ N14), G21 (= G15), I22 (= I16), L42 (= L35), L60 (≠ A60), D61 (≠ N61), I62 (= I62), N84 (≠ S88), G86 (= G90), L105 (≠ I109), I131 (≠ M134), Y146 (= Y149), K150 (= K153), P176 (= P179), G177 (≠ A180), I179 (= I182), T181 (= T184)
4iiuA Crystal structure of a putative 3-oxoacyl-[acyl-carrier protein]reductase from escherichia coli strain cft073 complexed with NADP+ at 2.1 a resolution
35% identity, 96% coverage: 5:249/254 of query aligns to 3:241/243 of 4iiuA
- active site: G13 (= G15), S140 (= S136), Y153 (= Y149), K157 (= K153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G11), S11 (≠ A13), K12 (≠ N14), G13 (= G15), I14 (= I16), H32 (≠ L34), H34 (≠ D36), D36 (= D38), D60 (≠ G74), V61 (≠ L75), N87 (≠ S88), A88 (= A89), G89 (= G90), I90 (≠ V91), S140 (= S136), Y153 (= Y149), K157 (= K153), P183 (= P179), G184 (≠ A180), I186 (= I182), T188 (= T184), M190 (= M186), I191 (≠ L187)
2q2qD Structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas putida (see paper)
32% identity, 96% coverage: 5:249/254 of query aligns to 5:251/255 of 2q2qD
- active site: G15 (= G15), S138 (= S136), Y151 (= Y149), K155 (= K153), R196 (≠ Q194)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), T13 (≠ A13), S14 (≠ N14), G15 (= G15), I16 (= I16), F36 (≠ D36), D59 (≠ N61), L60 (≠ I62), N86 (≠ S88), G88 (= G90), L109 (≠ I109), I136 (≠ M134), S138 (= S136), Y151 (= Y149), K155 (= K153), P181 (= P179), G182 (≠ A180), W183 (= W181), V184 (≠ I182), T186 (= T184), L188 (≠ M186), V189 (≠ L187)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
33% identity, 98% coverage: 1:249/254 of query aligns to 1:243/246 of 3osuA
Query Sequence
>PP_2784 FitnessBrowser__Putida:PP_2784
MSQLRTIVITGAANGIGRAVAESFAAQAEHLLILLDRDLATLQGWVTEGEFAARIETHQA
NIADLASLQLLFKGLADRVGFVDVLVNSAGVCDENEPEDLDNWHKVISINLNGTFYVTSL
CLPLMADGGRIVNMSSILGRAGKVRNTAYCASKHGIIGMTKALALDLAPRRITVNAILPA
WIDTPMLQGELAAQARIAGITHEQILRNAKKKLPLRRFIQGDEVAAMVRYLASPQASGVT
AQSLMIDGGAGLGM
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory