SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_2830 FitnessBrowser__Putida:PP_2830 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
24% identity, 69% coverage: 10:318/450 of query aligns to 56:374/534 of P53322

query
sites
P53322

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K283 (≠ A238) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
25% identity, 60% coverage: 48:319/450 of query aligns to 9:273/409 of 6e9nA

query
sites
6e9nA

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binding D-gluconic acid: F111 (= F150)

Sites not aligning to the query:

binding D-gluconic acid: 359

P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
25% identity, 60% coverage: 48:319/450 of query aligns to 20:292/430 of P0AA76

query
sites
P0AA76

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Y29 (≠ F57) binding D-galactonate
D31 (= D59) mutation to N: Loss of galactonate transport activity.
R32 (= R60) binding D-galactonate
Y64 (= Y92) binding D-galactonate
E118 (= E146) mutation to Q: Loss of galactonate transport activity.

Sites not aligning to the query:

358 binding D-galactonate

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
24% identity, 60% coverage: 48:319/450 of query aligns to 12:257/393 of 6e9oA

query
sites
6e9oA

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G

binding D-galactonic acid: Y21 (≠ F57), F114 (= F150), Q141 (≠ V177), Q228 (≠ H279)

Sites not aligning to the query:

binding D-galactonic acid: 320, 343

6zguA Crystal structure of a mfs transporter with bound 3-(2-methylphenyl) propanoic acid at 2.41 angstroem resolution
26% identity, 78% coverage: 73:421/450 of query aligns to 35:366/404 of 6zguA

query
sites
6zguA

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A

T

T

G

G

Y

F

F

A

L

V

G

G

V
x
L

C

G

V

L

A

G

N

S

I

F

L

L

S

M

G

G

P

P

L

L

G

A

G

T

A

Y

L

I

E

E

L

K

D

P

G

G

V

M

L

-

G

-

W

-

H

-

G

G

W

W

Q

R

W

Y

L

V

F

F

V

W

L

Y

E

C

G

G

L

V

P

A

A

M

V

G

A

I

L

M

A

A

Y

L

V

I

V

A

W

G

K

A

K

F

L

L

P

E

D

P

G

P

P

P

A

A

S

G

A

-

P

-

W

W

L

K

T

P

A

A

A

G

D

Y

A

T

Q

P

D

K

I

V

E

T

R

R

R

D

L

W

A

T

A

Y

E

E

Q

E

A

A

A

K

A

G

P

-

Q

-

S

-

K

-

L

-

G

-

Q

-

M

-

F

-

R

-

D

D

R

T

Q

K

I

F

W

W

L

L

A

L

I

Y

A

L

V

A

Y

Y

F

F

V

C

H

G

Q

S

I

F

T

A

I

G

Y

L

T

M

V

V

I

I

F

G

F

H

L

L

P

A

G

G

I

-

I

F

G

G

T

R

Y

D

A

A

A

G

L

L

S

T

P

A

F

M

Q

-

V

-

G

-

L

-

T

-

A

-

V

-

P

-

W

-

I

-

A

-

A

A

I

A

G

G

A

A

A

V

T

S

L

S

P

L

R

A

L

F

A

S

T

N

S

A

P

A

R

T

R
|
R

C

I

R

L

T

S

L

G

L

W

F

F

-

V

-

D

-

K

-

I

-

G

-

I

-

R

-

V

-

Y

-

F

-

A

L

L

G

F

L

A

L

L

T

Q

M

T

A

A

G

M

L

I

L

A

L

I

A

F

S

Q

L

L

T

G

N

G

S

S

F

V

I

V

G

G

L

L

-

S

-

I

-

V

-

A

-

I

-

V

I

I

G

G

F

W

S

N

L

Y

T

G

A

A

L

M

M
x
F

-

T

L

L

F

F

V

P

V

A

Q

T

S

C

I

L

I

Q

F

F

V

Y

F

G

P

P

S

T

S

A

R

Q

L

G

S

S

G

N

S

Y

A

G

L

L

A

-

G

-

L

-

A

-

F

L

V

F

T

T

T

A

C
|
C

G

G

L

L

F

-

G

A

G

G

F

F

V

A

G

G

P

P

S

W

V

V

M

G

G

G

L

W

I

L

E

K

Q

D

T

T

G

G

S

T

binding 3-(2-methylphenyl)propanoic acid: F54 (≠ Y92), Y113 (= Y156), L139 (≠ V177), R264 (= R332), F319 (≠ M369), C346 (= C400)

6zgtA Crystal structure of a mfs transporter with bound 2-naphthoic acid at 2.39 angstroem resolution
26% identity, 78% coverage: 73:421/450 of query aligns to 35:366/404 of 6zgtA

query
sites
6zgtA

D

E

L

F

G

G

L

W

S

S

A

R

A

A

A

E

Y

I

G

A

L

M

G

A

A

F

G

A

L

I

F

C

F
x
C

L

L

T

I

Y
x
F

A

G

L

L

A

M

E

T

V

F

P

P

S

A

N

G

L

R

I

L

M

S

H

D

R

K

V

M

G

G

A

P

R

R

F

-

W

-

I

-

T

-

R

K

I

V

M

V

I

M

T

T

W

G

G

G

-

V

L

L

S

A

A

I

G

G

M

F

-

I

-

L

-

S

A

G

F

F

V

I

Q

Q

G

S

E

K

T

Y

S

Q

F

L

Y

Y

I

I

M

T

R

Y

L

G

L

V

L

I

G

-

V

-

A

-

E

-

A

A

G

G

L

-

F

F

P

G

G

G

V

G

M

M

L

I

Y
|
Y

L

L

-

P

-

I

-

A

-

T

-

A

-

P

T

K

Y

W

W

W

F

P

D

D

R

R

E

-

Q

-

R

R

A

A

R

L

A

A

T

T

G

G

Y

F

F

A

L

V

G

G

V
x
L

C

G

V

L

A

G

N

S

I

F

L

L

S

M

G

G

P

P

L

L

G

A

G

T

A

Y

L

I

E

E

L

K

D

P

G

G

V

M

L

-

G

-

W

-

H

-

G

G

W

W

Q

R

W

Y

L

V

F

F

V

W

L

Y

E

C

G

G

L

V

P

A

A

M

V

G

A

I

L

M

A

A

Y

L

V

I

V

A

W

G

K

A

K

F

L

L

P

E

D

P

G

P

P

P

A

A

S

G

A

-

P

-

W

W

L

K

T

P

A

A

A

G

D

Y

A

T

Q

P

D

K

I

V

E

T

R

R

R

D

L

W

A

T

A

Y

E

E

Q

E

A

A

A

K

A

G

P

-

Q

-

S

-

K

-

L

-

G

-

Q

-

M

-

F

-

R

-

D

D

R

T

Q

K

I

F

W

W

L

L

A

L

I

Y

A

L

V

A

Y

Y

F

F

V

C

H

G

Q

S

I

F

T

A

I

G

Y

L

T

M

V

V

I

I

F

G

F

H

L

L

P

A

G

G

I

-

I

F

G

G

T

R

Y

D

A

A

A

G

L

L

S

T

P

A

F

M

Q

-

V

-

G

-

L

-

T

-

A

-

V

-

P

-

W

-

I

-

A

-

A

A

I

A

G

G

A

A

A

V

T

S

L

S

P

L

R

A

L

F

A

S

T

N

S

A

P

A

R

T

R
|
R

C

I

R

L

T

S

L

G

L

W

F

F

-

V

-

D

-

K

-

I

-

G

-

I

-

R

-

V

-

Y

-

F

-

A

L

L

G

F

L

A

L

L

T

Q

M

T

A

A

G

M

L

I

L

A

L

I

A

F

S

Q

L

L

T

G

N

G

S

S

F

V

I

V

G

G

L

L

-

S

-

I

-

V

-

A

-

I

-

V

I

I

G

G

F

W

S

N

L

Y

T

G

A

A

L

M

M
x
F

-

T

L

L

F

F

V

P

V

A

Q

T

S

C

I

L

I

Q

F

F

V

Y

F

G

P

P

S

T

S

A

R

Q

L

G

S

S

G

N

S

Y

A

G

L

L

A

-

G

-

L

-

A

-

F

L

V
x
F

T

T

T

A

C
|
C

G

G

L

L

F

-

G

A

G

G

F

F

V

A

G

G

P

P

S

W

V

V

M

G

G

G

L

W

I

L

E

K

Q

D

T

T

G

G

S

T

binding naphthalene-2-carboxylic acid: C51 (≠ F89), F54 (≠ Y92), Y113 (= Y156), L139 (≠ V177), R264 (= R332), F319 (≠ M369), F343 (≠ V397), C346 (= C400)

6zgsA Crystal structure of a mfs transporter with bound 3-phenylpropanoic acid at 2.39 angstroem resolution
26% identity, 78% coverage: 73:421/450 of query aligns to 35:366/404 of 6zgsA

query
sites
6zgsA

D

E

L

F

G

G

L

W

S

S

A

R

A

A

A

E

Y

I

G

A

L

M

G

A

A

F

G

A

L

I

F

C

L

L

T

I

Y
x
F

A

G

L

L

A

M

E

T

V

F

P

P

S

A

N

G

L

R

I

L

M

S

H

D

R

K

V

M

G

G

A

P

R

R

F

-

W

-

I

-

T

-

R

K

I

V

M

V

I

M

T

T

W

G

G

G

-

V

L

L

S

A

A

I

G

G

M

F

-

I

-

L

-

S

A

G

F

F

V

I

Q

Q

G

S

E

K

T

Y

S

Q

F

L

Y

Y

I

I

M

T

R

Y

L

G

L

V

L

I

G

-

V

-

A

-

E

-

A

A

G

G

L

-

F

F

P

G

G

G

V

G

M

M

L

I

Y
|
Y

L

L

-

P

-

I

-

A

-

T

-

A

-

P

T

K

Y

W

W

W

F

P

D

D

R

R

E

-

Q

-

R

R

A

A

R

L

A

A

T

T

G

G

Y

F

F

A

L

V

G

G

V
x
L

C

G

V

L

A

G

N

S

I

F

L

L

S

M

G

G

P

P

L

L

G

A

G

T

A

Y

L

I

E

E

L

K

D

P

G

G

V

M

L

-

G

-

W

-

H

-

G

G

W

W

Q

R

W

Y

L

V

F

F

V

W

L

Y

E

C

G

G

L

V

P

A

A

M

V

G

A

I

L

M

A

A

Y

L

V

I

V

A

W

G

K

A

K

F

L

L

P

E

D

P

G

P

P

P

A

A

S

G

A

-

P

-

W

W

L

K

T

P

A

A

A

G

D

Y

A

T

Q

P

D

K

I

V

E

T

R

R

R

D

L

W

A

T

A

Y

E

E

Q

E

A

A

A

K

A

G

P

-

Q

-

S

-

K

-

L

-

G

-

Q

-

M

-

F

-

R

-

D

D

R

T

Q

K

I

F

W

W

L

L

A

L

I

Y

A

L

V

A

Y

Y

F

F

V

C

H

G

Q

S

I

F

T

A

I

G

Y

L

T

M

V

V

I

I

F

G

F

H

L

L

P

A

G

G

I

-

I

F

G

G

T

R

Y

D

A

A

A

G

L

L

S

T

P

A

F

M

Q

-

V

-

G

-

L

-

T

-

A

-

V

-

P

-

W

-

I

-

A

-

A

A

I

A

G

G

A

A

A

V

T

S

L

S

P

L

R

A

L

F

A

S

T

N

S

A

P

A

R

T

R
|
R

C

I

R

L

T

S

L

G

L

W

F

F

-

V

-

D

-

K

-

I

-

G

-

I

-

R

-

V

-

Y

-

F

-

A

L

L

G

F

L

A

L

L

T

Q

M

T

A

A

G

M

L

I

L

A

L

I

A

F

S

Q

L

L

T

G

N

G

S

S

F

V

I

V

G

G

L

L

-

S

-

I

-

V

-

A

-

I

-

V

I

I

G

G

F

W

S

N

L

Y

T

G

A

A

L

M

M
x
F

-

T

L

L

F

F

V

P

V

A

Q

T

S

C

I

L

I

Q

F

F

V

Y

F

G

P

P

S

T

S

A

R

Q

L

G

S

S

G

N

S

Y

A

G

L

L

A

-

G

-

L

-

A

-

F

L

V

F

T

T

T

A

C
|
C

G

G

L

L

F

-

G

A

G

G

F

F

V

A

G

G

P

P

S

W

V

V

M

G

G

G

L

W

I

L

E

K

Q

D

T

T

G

G

S

T

binding hydrocinnamic acid: F54 (≠ Y92), Y113 (= Y156), L139 (≠ V177), R264 (= R332), F319 (≠ M369), C346 (= C400)

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
24% identity, 81% coverage: 86:450/450 of query aligns to 84:467/493 of Q03567

query
sites
Q03567

G

G

L

M

F

V

F

L

L

S

T

S

Y

F

A

F

L

Y

A

G

E

Y

V

I

P

G

S

S

N

Q

L

I

I

I

M

G

H

G

R

H

V

L

G

A

A

S

R

R

F

Y

W

G

I

G

T

K

R

R

I

V

M

V

I

F

T

V

W

T

G

I

L

L

L

G

S

S

A

A

G

L

M

L

A

T

F

L

-

L

-

N

-

P

V

V

Q

A

G

A

E

R

T

T

S

S

F

E

Y

Y

-

A

-

L

-

A

I

I

M

L

R

R

L

A

L

I

G

G

V

F

A

L

E

Q

A

G

G

A

L

T

F

F

P

P

G

A

V

M

M

H

L

T

Y

M

L

W

T

S

Y

V

W

W

F

G

D

P

R

P

E

L

Q

E

R

L

A

S

R

V

A

L

T

T

G

G

Y

V

F

T

L

Y

L

A

G

G

V

A

C

Q

V

I

A

G

N

N

I

V

L

I

S

V

G

L

P

P

L

L

G

S

G

G

A

F

L

L

L

C

E

E

-

Y

-

G

L

F

D

D

G

G

V

-

L

-

G

-

W

-

H

-

G

G

W

W

Q

P

W

S

L

I

F

F

V

Y

L

I

E

I

G

G

L

V

P

F

A

G

V

V

A

L

L

W

A

T

Y

A

V

V

V

W

W

W

K

Y

K

V

L

S

P

S

D

D

G

K

P

P

A

A

S

T

A

H

P

P

W

R

L

I

T

T

A

P

A

E

D

E

A

K

Q

Q

D

Y

I

I

-

V

-

T

-

A

-

V

E

E

R

A

R

S

L

M

A

G

A

K

E

D

Q

T

A

G

A

K

A

V

P

P

Q

S

Q

T

S

P

K

W

L

I

G

-

Q

K

M

I

F

L

R

T

D

S

R

P

Q

A

I

V

W

W

L

A

A

C

I

W

A

A

V

G

Y

H

F

F

V

A

H

G

Q

D

I

W

T

G

I

A

Y

Y

T

T

V

M

I

L

F

V

F

S

L

L

P

P

G

S

I

F

I

L

G

K

T

D

Y

V

A

L

A

G

L

L

S

N

P

L

F

S

Q

S

V

L

G

G

L

A

L

V

T

A

A

S

V

I

P

P

W

Y

I

I

-

A

-

Y

-

F

-

L

-

A

-

I

-

N

A

A

A

G

I

V

G

L

A

A

A

D

T

T

L

L

P

R

R

S

-

K

-

G

-

I

L

L

A

S

T

T

-

L

S

N

P

T

R

R

C

A

R

A

T

M

L

L

L

V

F

A

L

L

-

I

-

G

-

Q

G

G

L

I

L

F

T

L

M

V

A

A

A

S

G

G

L

Y

L

C

L

G

A

C

S

G

L

Q

T

D

N

V

S

L

F

V

I

I

G

I

L

F

I

I

-

T

-

C

G

G

F

M

S

A

L

I

T

S

A

G

L

L

M

Q

L

Y

-

A

-

G

F

F

V

V

Q

N

S

Y

I

L

I

E

F

I

V

A

F

P

P

P

S

-

R

-

L

F

S

S

G

G

S

T

A

V

L

M

A

G

A

T

G

G

L

-

A

-

F

-

V

-

T

N

T

T

C

I

G

S

L

A

F

L

G

A

G

G

F

I

V

I

G

S

P

P

S

A

V

V

M

S

G

S

L

Y

I

L

-

T

-

P

E

N

Q

G

T

T

T

Q

G

E

S

E

T

W

R

Q

N

M

G

V

L

L

W

W

I

L

I

T

A

A

A

G

L

I

L

L

V

T

C

I

A

G

A

A

L

L

V

L

S

F

T

S

R

I

L

F

R

A

Q

S

G

G

Q

E

E

V

Q

Q

P

P

Sites not aligning to the query:

69 modified: carbohydrate, N-linked (GlcNAc...) asparagine

6g9xA Crystal structure of a mfs transporter at 2.54 angstroem resolution (see paper)
26% identity, 74% coverage: 90:421/450 of query aligns to 49:358/396 of 6g9xA

query
sites
6g9xA

L

L

T

I

Y

F

A

G

L

L

A

M

E

T

V

F

P

P

S

A

N

G

L

R

I

L

M

S

H

D

R

K

V

M

G

G

A

P

R

R

F

-

W

-

I

-

T

-

R

K

I

V

M

V

I

M

T

T

W

G

G

G

-

V

L

L

S

A

A

I

G

G

M

F

-

I

-

L

-

S

A

G

F

F

V

I

Q

Q

G

S

E

K

T

Y

S

Q

F

L

Y

Y

I

I

M

T

R

Y

L

G

L

V

L

I

G

-

V

-

A

-

E

-

A

A

G

G

L

-

F

F

P

G

G

G

V

G

M

M

L

I

Y
|
Y

L

L

-

P

-

I

-

A

-

T

-

A

-

P

T

K

Y

W

W

W

F

P

D

D

R

R

E

-

Q

-

R

R

A

A

R

L

A

A

T

T

G

G

Y

F

F

A

L

V

G

G

V
x
L

C

G

V

L

A

G

N

S

I

F

L

L

S

M

G

G

P

P

L

L

G

A

G

T

A

Y

L

I

E

E

L

-

D

-

G

-

V

-

L

-

G

-

W

-

H

-

G

-

W

W

Q

R

W

Y

L

V

F

F

V

W

L

Y

E

C

G

G

L

V

P

A

A

M

V

G

A

I

L

M

A

A

Y

L

V

I

V

A

W

G

K

A

K

F

L

L

P

E

D

P

G

P

P

P

A

A

S

G

A

-

P

-

W

W

L

K

T

P

A

A

A

G

D

A

A

A

Q

P

D

K

I

V

E

T

R

R

R

D

L

W

A

T

A

Y

E

E

Q

E

A

A

A

K

A

G

P

-

Q

-

S

-

K

-

L

-

G

-

Q

-

M

-

F

-

R

-

D

D

R

T

Q

K

I

F

W

W

L

L

A

L

I

Y

A

L

V

A

Y

Y

F

F

V

C

H

G

Q

S

I

F

T

A

I

G

Y

L

T

M

V

V

I

I

F

G

F

H

L

L

P

A

G

G

I

-

I

F

G

G

T

R

Y

D

A

A

A

G

L

L

S

T

P

A

F

M

Q

-

V

-

G

-

L

-

T

-

A

-

V

-

P

-

W

-

I

-

A

-

A

A

I

A

G

G

A

A

A

V

T

S

L

S

P

L

R

A

L

F

A

S

T

N

S

A

P

A

R

T

R
|
R

C

I

R

L

T

S

L

G

L

W

F

F

L

V

G

D

-

K

-

I

-

G

-

I

-

R

-

V

-

Y

-

F

-

A

-

L

-

F

L

A

L

L

T

Q

M

T

A

A

G

M

L

I

L

A

L

I

A

F

S

Q

L

L

T

G

N

G

S

S

F

V

I

V

G

G

L

L

-

S

-

I

-

V

-

A

-

I

-

V

I

I

G

G

F

W

S

N

L
x
Y

T

G

A

A

L

M

M
x
F

-

T

L

L

F

F

V

P

V

A

Q

T

S

C

I

L

I

Q

F

F

V

Y

F

G

P

P

S

T

S

A

R

Q

L

G

S

S

G

N

S

Y

A

G

L

L

A

-

G

-

L

-

A

-

F

L

V

F

T

T

T

A

C
|
C

G

G

L

L

F

-

G

A

G

G

F

F

V

A

G

G

P

P

S

W

V

V

M

G

G

G

L

W

I

L

E

K

Q

D

T

T

G

G

S

T

binding 2-sulfanylbenzoic acid: Y110 (= Y156), L136 (≠ V177), R256 (= R332), Y307 (≠ L365), F311 (≠ M369), C338 (= C400)

A0LNN5 L-lactate transporter; SfMCT from Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) (see paper)
26% identity, 78% coverage: 73:421/450 of query aligns to 41:382/412 of A0LNN5

query
sites
A0LNN5

D

E

L

F

G

G

L

W

S

S

A

R

A

A

A

E

Y

I

G

A

L

M

G

A

A

F

G

A

L

I

F

C

L

L

T

I

Y

F

A

G

L

L

A

M

E

T

V

F

P

P

S

A

N

G

L

R

I

L

M

S

H

D

R

K

V

M

G

G

A

P

R

R

F

-

W

-

I

-

T

-

R

K

I

V

M

V

I

M

T

T

W

G

G

G

-

V

L

L

S

A

A

I

G

G

M

F

-

I

-

L

-

S

A

G

F

F

V

I

Q

Q

G

S

E

K

T

Y

S

Q

F

L

Y

Y

I

I

M

T

R

Y

L

G

L

V

L

I

G

-

V

-

A

-

E

-

A

A

G

G

L

-

F

F

P

G

G

G

V

G

M

M

L

I

Y
|
Y

L

L

-

P

-

I

-

A

-

T

-

A

-

P

T

K

Y

W

W

W

F

P

D

D

R

R

E

-

Q

-

R

R

A

A

R

L

A

A

T

T

G

G

Y

F

F

A

L

V

G

G

V
x
L

C

G

V

L

A

G

N

S

I

F

L

L

S

M

G

G

P

P

L

L

G

A

G

T

A

Y

L

I

E

E

L

K

D

P

G

G

V

M

L

-

G

-

W

-

H

-

G

G

W

W

Q

R

W

Y

L

V

F

F

V

W

L

Y

E

C

G

G

L

V

P

A

A

M

V

G

A

I

L

M

A

A

Y

L

V

I

V

A

-

G

-

A

-

F

-

L

-

E

-

P

-

A

-

G

W

W

K

K

K

-

L

-

P

P

D

A

G

G

P

Y

A

T

S

P

A

P

P

A

W

P

L

P

T

A

A

G

A

A

D

A

A

A

Q

P

D

K

I

V

E

T

R

R

R

D

L

W

A

T

A

Y

E

E

Q

E

A

A

A

K

A

G

P

-

Q

-

S

-

K

-

L

-

G

-

Q

-

M

-

F

-

R

-

D

D

R

T

Q

K

I

F

W

W

L

L

A

L

I

Y

A

L

V

A

Y

Y

F

F

V

C

H

G

Q

S

I

F

T

A

I

G

Y

L

T

M

V

V

I

I

F

G

F
x
H

L

L

P

A

G

G

I

-

I

F

G

G

T
x
R

Y
x
D

A

A

A

G

L

L

S

T

P

A

F

M

Q

-

V

-

G

-

L

-

T

-

A

-

V

-

P

-

W

-

I

-

A

-

A

A

I

A

G

G

A

A

A

V

T

S

L

S

P

L

R

A

L

F

A

S

T
x
N

S

A

P

A

R

T

R
|
R

C

I

R

L

T

S

L

G

L

W

F

F

L

V

G

D

-

K

-

I

-

G

-

I

-

R

-

V

-

Y

-

F

-

A

-

L

-

F

L

A

L

L

T

Q

M

T

A

A

G

M

L

I

L

A

L

I

A

F

S

Q

L

L

T

G

N

G

S

S

F

V

I

V

G

G

L

L

-

S

-

I

-

V

-

A

-

I

-

V

I

I

G

G

F

W

S

N

L
x
Y

T

G

A

A

L

M

M
x
F

-

T

L

L

F

F

V

P

V

A

Q

T

S

C

I

L

I

Q

F

F

V

Y

F

G

P

P

S

T

S

A

R

Q

L

G

S

S

G

N

S

Y

A

G

L

L

A

-

G

-

L

-

A

-

F

L

V
x
F

T

T

T

A

C
|
C

G

G

L

L

F

-

G

A

G

G

F

F

V

A

G

G

P

P

S

W

V

V

M

G

G

G

L

W

I

L

E
x
K

Q
x
D

T

T

G

G

S

T

Y119 (= Y156) binding (S)-lactate; mutation Y->A,F: Loss of transport activity.
L145 (≠ V177) mutation to A: Strong decrease in transport activity.
H250 (≠ F289) mutation to A: Strong decrease in transport activity.; mutation to F: Loss of transport activity.
R256 (≠ T296) mutation to A: No change in transport activity.; mutation to D: Increases transport activity.
D257 (≠ Y297) mutation to A: Loss of transport activity.
N276 (≠ T328) mutation to A: Loss of transport activity.
R280 (= R332) binding (S)-lactate; mutation to A: Abolishes L-lactate binding and L-lactate transport.
Y331 (≠ L365) mutation to A: Loss of transport activity.; mutation to F: No change in transport activity.
F335 (≠ M369) mutation to A: Increases transport activity.
F359 (≠ V397) mutation to A: Loss of transport activity.
C362 (= C400) mutation to A: Decrease in transport activity.
K377 (≠ E416) mutation K->A,D: No change in transport activity.
D378 (≠ Q417) mutation to A: Loss of transport activity.

Sites not aligning to the query:

28 L→A: Loss of transport activity.
383 Y→A: Loss of transport activity.; Y→F: Strong decrease in transport activity.

8u3gA Structure of naag-bound sialin
21% identity, 87% coverage: 60:449/450 of query aligns to 22:422/427 of 8u3gA

query
sites
8u3gA

R

R

T

V

N

N

I

L

A

S

L

V

A

A

K

L

A

V

S

D

M

M

-

V

-

D

-

S

-

T

G

G

I

K

D

K

L

Y

G

Q

L

W

S

D

A

A

A

E

A

T

Y

Q

G

G

L

W

G

I

A

L

G

G

L

S

F

F

L

Y

T

G

Y

Y

A

I

L

I

A

T

E

Q

V

I

P

P

S

G

N

G

L

Y

I

V

M

A

H

S

R

K

V

I

G

G

A

G

R

K

F

-

W

-

I

-

T

-

R

-

I

M

M

L

I

L

T

G

W

F

G

G

L

I

L

L

S

G

A

T

G

A

-

V

-

L

-

T

-

L

-

F

-

T

-

P

M

I

A

A

F

A

V

D

Q

L

G

G

E

V

T

G

S

P

F

L

Y

I

I

V

M

L

R

R

L

A

L

L

L

E

G

G

V

L

A

G

E

E

A

G

G

V

L

T

F
|
F

P

P

G

A

V

M

M

H

L

A

Y

M

L

W

T

S

Y

S

W

W

F

A

D

P

R

P

E

L

Q

E

R

R

A

S

R

K

A

L

T

L

G

S

Y

I

F

S

L

Y

L

A

G

G

V

A

C

Q

V

L

A

G

N

T

I

V

L

I

S

S

G

L

P

P

L

L

G

S

G

G

A

I

L

I

L

C

E

Y

L

-

D

-

G

-

V

-

L

-

G

-

W

Y

H

M

G

N

W

W

Q

T

W

Y

L

V

F

F

V

Y

L

F

E

F

G

G

L

T

P

I

A

G

V

I

A

-

L

F

A

W

Y

F

V

L

W

W

K

I

K

W

L

L

-

V

P

S

D

D

G

T

P

P

A

Q

S

K

A

H

P

K

W

R

L

I

T

S

A

H

A

Y

D

E

A

K

Q

E

D

Y

I

I

E

L

R

S

L

L

A

R

A

-

E

N

Q

Q

A

L

A

S

P

Q

Q

K

Q

S

S

V

K

P

L

W

G

V

Q

P

M

I

F

L

R

K

D

S

R

L

Q

P

I

L

W

W

L

A

A

I

I

V

A

V

V

A

Y

H

F

F

V

S

H

Y

Q

N

I

W

T

T

I

F

Y

Y

T

T

V

L

I

L

F

T

F

L

L

L

P

P

G

T

I

Y

I

M

G

K

T

E

Y

I

A

L

A

R

L

F

S

N

P

V

F

Q

Q

E

V

N

G

G

L

F

L

L

T

S

A

S

V

L

P

P

W

Y

I

L

A

G

A

S

A

W

I

L

G

C

A

M

A

I

T

L

L

S

P

G

R

Q

L

A

A

A

T

D

S

N

P

L

R

R

R

A

-

K

-

W

-

N

-

F

-

S

-

T

-

L

C

C

-

V

-

R

R

R

T

I

L

F

L

S

F

L

L

I

G

G

L

M

L

I

T

G

M

P

A

A

A

V

G

F

L

L

L

V

L

A

A

A

S

-

L

-

T

-

N

-

S

G

F

F

I

I

G

G

L

-

I

C

G

D

F

Y

S

S

L

L

T

-

A

A

L

V

M

A

L

F

F

L

V

T

V

I

Q

S

S

T

I

T

I

L

F

G

V

G

F

F

P

C

S

S

R

G

L

F

S
|
S

G

I

S

N

A
x
H

L

L

A

D

-

I

-

A

-

P

-

S

-

Y

A

A

G

G

-

I

-

L

L
|
L

A

G

F

I

V

T

T

N

T

T

C

F

G

A

L

T

F

I

G

P

G

G

F

M

V

V

G

G

P

P

S

V

V

I

M

A

G

K

L

S

I

L

-

T

-

P

E

D

Q

N

T

T

T

V

G

G

S

E

T

W

R

Q

N

T

G

V

L

F

W

Y

I

I

I

A

A

A

L

I

L

N

V

V

C

F

A

G

A

A

L

I

V

F

S

F

T

T

R

L

L

F

R

A

Q

K

G

G

Q

E

E

V

Q

Q

binding aspartic acid: F118 (= F150)
binding glutamic acid: S350 (= S386), H353 (≠ A389), L365 (= L394)

Sites not aligning to the query:

binding aspartic acid: 15, 19

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
20% identity, 56% coverage: 63:313/450 of query aligns to 90:335/495 of Q8BN82

query
sites
Q8BN82

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H183 (≠ M154) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
20% identity, 86% coverage: 63:449/450 of query aligns to 90:483/495 of Q9NRA2

query
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Q9NRA2

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K136 (≠ R109) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ M154) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AT 169:170) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ IE 243:244) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLR-N 268:272 (≠ RRLAAE 245:250) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G306) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P312) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G340) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

8u3hA Structure of fmoc-leu-oh bound sialin
20% identity, 87% coverage: 60:449/450 of query aligns to 22:420/425 of 8u3hA

query
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