SitesBLAST
Comparing PP_4737 FitnessBrowser__Putida:PP_4737 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (≠ A34), P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), W322 (≠ F380), E413 (= E474), H414 (= H475), N450 (= N511)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R376), H369 (= H429), H376 (= H436), H414 (= H475)
- binding manganese (ii) ion: H369 (= H429), H376 (= H436), E413 (= E474)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
25% identity, 54% coverage: 12:515/936 of query aligns to 15:463/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ F69), G75 (≠ A71), S76 (≠ A72), G77 (= G73), T78 (= T74), G79 (≠ S75), L80 (= L76), A83 (≠ Q79), C84 (≠ A80), P137 (= P131), G138 (≠ A132), E139 (≠ S133), A142 (= A136), T143 (≠ C137), G146 (= G141), N147 (≠ I142), S149 (≠ A144), T150 (≠ N145), A152 (= A147), G153 (≠ S148), E203 (= E249), G204 (= G250), I209 (= I255), E422 (= E474), H423 (= H475)
- binding fe (iii) ion: H377 (= H429), H384 (= H436), E422 (= E474)
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (≠ A34), P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), W323 (≠ F380), E414 (= E474), H415 (= H475), N451 (= N511)
- binding manganese (ii) ion: H370 (= H429), H377 (= H436), E414 (= E474)
- binding pyruvic acid: R319 (= R376), H370 (= H429), H377 (= H436), H415 (= H475)
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ T74), R317 (= R376), W321 (≠ F380), H368 (= H429), H375 (= H436), H413 (= H475)
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), W321 (≠ F380), Y322 (≠ P381), E412 (= E474), H413 (= H475), N449 (= N511)
- binding manganese (ii) ion: H368 (= H429), H375 (= H436), E412 (= E474)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), W322 (≠ F380), E413 (= E474), H414 (= H475), N450 (= N511)
- binding lactic acid: R318 (= R376), H369 (= H429), H376 (= H436), H414 (= H475)
- binding manganese (ii) ion: H369 (= H429), H376 (= H436), E413 (= E474)
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R376), W322 (≠ F380), H369 (= H429), H376 (= H436), H414 (= H475)
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), W322 (≠ F380), E413 (= E474), N450 (= N511)
- binding manganese (ii) ion: H369 (= H429), H376 (= H436), E413 (= E474)
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R376), H369 (= H429), H376 (= H436), H414 (= H475)
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), W322 (≠ F380), E413 (= E474), H414 (= H475), N450 (= N511)
- binding manganese (ii) ion: H369 (= H429), H376 (= H436), E413 (= E474)
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R376), W322 (≠ F380), H369 (= H429), H376 (= H436), H413 (= H475)
- binding flavin-adenine dinucleotide: E32 (≠ A34), P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), W322 (≠ F380), E412 (= E474), H413 (= H475), N449 (= N511)
- binding manganese (ii) ion: H369 (= H429), H376 (= H436), E412 (= E474)
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (= R376), W322 (≠ F380), S336 (≠ I395), H369 (= H429), H376 (= H436), H413 (= H475)
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), E412 (= E474), N449 (= N511)
- binding manganese (ii) ion: H369 (= H429), H376 (= H436), E412 (= E474)
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), Y324 (≠ P381), H370 (= H429), E414 (= E474), N451 (= N511)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R376), W323 (≠ F380), H415 (= H475)
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:456/456 of 8jdqA
- binding (2R)-2-hydroxy-4-methylpentanoic acid: R319 (= R376), W323 (≠ F380), H370 (= H429), H415 (= H475)
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), H370 (= H429), E414 (= E474), N451 (= N511)
8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:456/456 of 8jdoA
- binding (2R)-2-hydroxyhexanoic acid: R319 (= R376), W323 (≠ F380), H415 (= H475)
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), Y324 (≠ P381), H370 (= H429), E414 (= E474), N451 (= N511)
8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:456/456 of 8jdnA
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), H370 (= H429), E414 (= E474), N451 (= N511)
- binding (2R)-2-oxidanylpentanoic acid: R319 (= R376), W323 (≠ F380), H415 (= H475)
8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:456/456 of 8jdgA
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), H370 (= H429), E414 (= E474), N451 (= N511)
- binding (2R)-2-oxidanylbutanoic acid: R319 (= R376), H415 (= H475)
8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:456/456 of 8jdbA
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), Y324 (≠ P381), H370 (= H429), E414 (= E474), N451 (= N511)
- binding (2R)-2-oxidanyloctanoic acid: V75 (≠ T74), R319 (= R376), W323 (≠ F380), H415 (= H475)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
26% identity, 53% coverage: 23:516/936 of query aligns to 22:465/465 of 3pm9A
- active site: A149 (= A147), L159 (≠ N157)
- binding flavin-adenine dinucleotide: P69 (≠ F69), Q70 (≠ R70), G71 (≠ A71), G72 (≠ A72), N73 (≠ G73), T74 (= T74), G75 (≠ S75), L76 (= L76), G79 (≠ Q79), Q80 (≠ A80), L91 (= L89), L133 (≠ P131), G134 (≠ A132), A135 (≠ S133), C139 (= C137), T140 (≠ K138), G142 (= G140), G143 (= G141), S146 (≠ A144), T147 (≠ N145), A149 (= A147), G150 (≠ S148), E200 (= E249), G201 (= G250), I205 (≠ F254), I206 (= I255), E423 (= E474)
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
26% identity, 52% coverage: 31:516/936 of query aligns to 29:455/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ F69), T71 (≠ R70), G72 (≠ A71), T73 (≠ A72), G74 (= G73), G78 (≠ S77), V79 (≠ G78), L90 (= L89), P132 (= P131), G133 (≠ A132), A134 (≠ S133), G140 (= G141), M141 (≠ I142), A143 (= A144), T144 (≠ N145), A146 (= A147), S147 (= S148), E200 (= E249), G201 (= G250), I206 (= I255), H369 (= H429), E413 (= E474), N450 (= N511)
- binding deaminohydroxyvaline: R319 (= R376), H414 (= H475)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
26% identity, 51% coverage: 41:518/936 of query aligns to 40:458/459 of P9WIT1
- K354 (≠ E407) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
25% identity, 54% coverage: 12:512/936 of query aligns to 71:513/521 of Q8N465
- S109 (≠ E50) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ T68) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (≠ A72) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V88) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ N94) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ A118) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (vs. gap) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A144) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (= A170) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G172) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (= D365) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R376) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- T390 (≠ F380) binding ; binding ; mutation to A: Significantly reduced catalytic activity.
- V399 (= V393) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (≠ I395) binding ; binding ; mutation to A: Loss of catalytic activity.
- R419 (≠ I413) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ Y421) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H429) binding ; mutation to A: Loss of catalytic activity.
- G436 (≠ L431) to V: slight reduction in catalytic activity
- N439 (= N434) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H436) binding ; mutation to A: Loss of catalytic activity.
- N443 (≠ V438) binding ; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ F439) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ Q441) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E474) binding ; mutation to A: Loss of catalytic activity.
- H476 (= H475) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- G477 (= G476) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
21% identity, 51% coverage: 37:512/936 of query aligns to 64:492/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Query Sequence
>PP_4737 FitnessBrowser__Putida:PP_4737
MSLPAAFLRDAERLIPAERRFDDPTSTLAFGTDASFYRLIPKLVVRVESEDEVVGLIKLA
QRERVPVTFRAAGTSLSGQAISDSVLIVLGDNWNGREIRRQGEQIRLQPGVIGAQANAWL
APFGRKIGPDPASINACKIGGIVANNASGMCCGTAQNTYHTLAGLRLVLADGTRLDSEDP
ASVAAFESSHAELLEALARLGRETRANTALAERIRHKYRLKNTTGLSLNALVDYDQPLDI
LQHLLVGSEGTLGFISAVTYDTVPDHPHKASALLVFPSVESCCRAVPVLKQQPVSAVELL
DRRSLRSVQNMPGMPLWVKGLSDNACALLIESRAASQSLLHEQLQQIMASIADFPLEQQV
DFSEDPAVYNQLWKIRKDTFPAVGAVRQTGTTVIIEDVTFPVEQLAEGVNRLIQLFDKHH
YDEAIIFGHALEGNLHFVFTQGFNSAEEVARYQAFMDDVAQLVAVEFGGSLKAEHGTGRN
MAPFVELEWGHDAYQLMWKLKRLLDPNGILNPDVVLSEDPDIHLKNLKPLPAADKIVDKC
IECGFCEPVCPSKGLTLSPRQRIVMWRDIQAKQRAGIDTRELMQSYQYQGIDTCAATGLC
AQRCPVGINTGELVKKLRSQAADHAKTADWLAEHFHTALSGARLTLTAANTARKLLGAPR
LGRLSASLSKASKGRLPQWTPAMPQPLRPLAFGATSNDARPRVVYLAACVSRVMGPAYAD
REQSSLLDKTRALLEKAGYQVVFPDNADSLCCGQPFASKGYPEQAEHKRQELITALLHAS
RGGLDPIYCDTSPCTLRLVQDLSDTRLDLYDPVRFIRTHLLDRLEFTPQDEPVAVHVTCS
TQHLGESQALIDLARRCSKQVVIPEGIHCCGFAGDKGFTTPELNAHSLRSLKDAVQYCSE
GISTSRTCEIGLSSHSGIDYHGLVYLVDRVTRPRSL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory