SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_5257 FitnessBrowser__Putida:PP_5257 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
24% identity, 82% coverage: 35:390/432 of query aligns to 28:386/824 of Q8GAI3

query
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Q8GAI3

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W66 (≠ G72) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
H67 (≠ R73) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.

5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
23% identity, 86% coverage: 31:400/432 of query aligns to 23:379/383 of 5i39A

query
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active site: F66 (≠ N74), Q69 (≠ N79), A70 (= A80), Q248 (vs. gap), P267 (≠ D285)
binding flavin-adenine dinucleotide: V30 (≠ I38), G31 (= G39), G33 (= G41), I34 (= I42), L35 (≠ T43), V53 (≠ L61), E54 (= E62), K55 (≠ A63), Q62 (≠ G70), S63 (= S71), F66 (≠ N74), Y67 (≠ V75), Q69 (≠ N79), A196 (≠ S211), A197 (≠ V212), G226 (≠ T240), G227 (= G241), W229 (= W248), Q248 (vs. gap), Q250 (vs. gap), G321 (= G343), M323 (≠ I345), T348 (≠ N369), G349 (= G370), W350 (≠ R371), G351 (= G372), M352 (≠ N373), T353 (= T374)

5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
25% identity, 51% coverage: 32:252/432 of query aligns to 16:230/433 of 5hxwA

query
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active site: F58 (≠ N74), Q61 (≠ N79), A62 (= A80)
binding flavin-adenine dinucleotide: V22 (≠ I38), G23 (= G39), G25 (= G41), I26 (= I42), L27 (≠ T43), E46 (= E62), K47 (≠ A63), E53 (≠ G69), Q54 (≠ G70), S55 (= S71), R57 (= R73), F58 (≠ N74), Y59 (≠ V75), G60 (= G76), Q61 (≠ N79), A188 (≠ S211), A189 (≠ V212), G218 (≠ T240), G219 (= G241), W221 (≠ Y243)

Sites not aligning to the query:

active site: 240, 284, 288
binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
binding flavin-adenine dinucleotide: 240, 242, 331, 371, 373, 398, 399, 400, 401, 402, 403

Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
23% identity, 91% coverage: 9:401/432 of query aligns to 27:429/866 of Q9UI17

query
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S

L

A

A

A

G

L

L

L

Y

D

N

R

P

R

G

A

D

G

G

T

H

I

I

N

D

P

P

M

Y

G

S

Y

L

T

T

Q

M

G

A

L

L

A

A

A

G

V

A

T

R

R

K

L

C

G

G

G

A

K

L

I

L

F

K

Q

Y

Q

P

S

A

S

P

V
|
V

E

T

G

S

L

L

E

K

R

A

E

R

G

S

D

D

G

G

-

T

W

W

R

D

V

V

K

E

T

T

A

P

R

Q

G

G

A

S

V

M

R

R

A

A

E

N

K

R

V

I

V

V

I

N

S

A

T

A

G

G

A

F

Y

W

T

A

E

R

-

E

-

V

G

G

D

K

W

M

S

I

N

G

L

L

Q

E

K

H

Q

P

F

L

F

I

R

P

G

V

Y

Q

Y

H

Y

Q

Q

Y

V

V

A

V

S

T

K

S

P

T

L

I

Q
x
S

G

E

I

V

A

K

A

A

D

L

K

K

-

R

-

E

-

L

-

P

V

V

L

L

P

R

H

D

G

L

Q

E

G

G

S

S

W

Y

D

Y

T

L

R

R

T

Q

V

E

L

R

S

D

S

G

I

L

-

L

-

F

-

G

R

P

R

Y

D

E

D

S

Q

Q

G

E

R

K

L

M

L

K

L

V

G

Q

S

D

L

S

G

W

R

V

V

T

D

N

N

G

K

V

P

P

A

P

W

G

F

F

-

G

-

K

-

E

-

L

V

F

R

E

S

S

W

D

A

L

D

D

R

R

I

I

Q

M

S

E

H

H

-

I

-

K

-

A

-

M

-

E

Y

M

Y

V

P

P

E

V

L

L

G

K

K

K

V

A

E

D

W

I

E

I

M

N

H

V

W

V

T

N

G

G

C

P

I

I

D

T

F

Y

T

S

P

P

D

D

H

-

L

I

M

L

R

P

L

M

F

V

E

G

P

P

A

H

P

Q

G

G

L

V

-

R

-

N

-

Y

-

W

V

V

A

A

V

I

T

-

G

G

Y
x
F

N

-

G
|
G

R
x
Y

G
|
G

N
x
I

T
x
I

T

H

G

A

T

G

V

G

I

V

G

G

R

K

A

Y

F

L

A

S

E

D

F

W

L

I

L

L

K

H

G

G

E

E

A

P

D

P

S

F

L

D

P

L

I

I

P

E

F

L

S

D

P

P

CV 59:60 (≠ IT 42:43) binding
EK 80:81 (≠ EA 62:63) binding
87:95 (vs. 69:77, 33% identical) binding
H91 (≠ R73) modified: Tele-8alpha-FAD histidine
H109 (≠ L105) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
V219 (= V212) binding
S279 (≠ Q269) to P: in dbSNP:rs532964
F-GYGII 397:402 (≠ YNGRGNT 368:374) binding

Sites not aligning to the query:

530 A → G: in dbSNP:rs1805073
646 S → P: in dbSNP:rs1805074

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
25% identity, 82% coverage: 32:387/432 of query aligns to 1:344/363 of 7cyxA

query
sites
7cyxA

K

K

A

Y

D

D

V

V

C

A

V

I

I
|
I

G
|
G

G

G

G
|
G

I
x
V

T
x
I

G

G

L

S

S

S

A

V

A

A

I

H

H

F

L

L

L

A

E

E

Q

R

G

G

K

H

S

K

V

V

I

A

V

I

L
x
V

E
|
E

A
x
K

W

Q

K

S

I

I

G

A

H

S

G
x
E

G
x
A

S
|
S

G

K

R

A

N
x
A

V

A

G
|
G

L
|
L

V

L

N

-

A

-

G

G

T

V

W

A

I

Y

R

N

P

P

D

L

D

F

V

E

E

L

A

A

T

R

L

E

G

S

Q

R

K

A

Q

I

G

F

S

P

R

Q

L

L

N

A

K

A

V

V

L

L

G

R

E

-

A

-

P

-

A

-

E

-

V

-

F

-

A

-

M

-

I

-

E

E

R

K

L

T

G

G

I

V

D

D

C

I

Q

G

A

Y

Q

E

H

E

K

K

G

G

T

I

L

Y

H

R

M

I

A

A

H

Q

N

N

A

E

T

D

G

E

I

K

A

E

D

R

L

I

E

L

A

H

R

I

H

M

E

D

Q

W

W

Q

R

Q

R

K

R

T

G

G

A

E

D

D

V

S

E

Y

L

F

L

L

T

T

G

G

A

D

Q

H

C

V

Q

R

E

E

-

K

-

E

-

P

Y

Y

C

L

G

S

T

E

D

S

K

I

I

I

S

G

A

A

A

V

L

Y

D

P

R

K

R

D

A

G

G

H

T

V

I

I

N

A

P

P

M

E

G

-

Y

L

T

T

Q

K

G

A

L

F

A

A

A

H

A

S

V

A

T

A

R

I

L

S

G

G

G

A

K

D

I

I

F

Y

Q

E

Q

Q

S

T

S

E

V
|
V

E

F

G

D

L

I

E

R

R

I

E

E

G

N

D

N

G

K

W

V

R

T

-

G

V

V

K

I

T

T

A

S

R

E

G

G

A

I

V

V

R

T

A

C

E

E

K

K

V

V

I

I

S

A

T
x
G

G
|
G

A

S

Y
x
W

T

S

E

-

G

-

D

-

W

-

S

T

N

K

L

L

Q

L

K

S

Q

Y

F

F

F

H

R

R

G

D

Y

W

Y

-

Q

-

V

-

A

G

S

T

K

Y

P

P

L

V

Q

K

G

G

-

E

-

V

-

A

I

V

A

R

A

S

D

R

K

K

V

Q

L

L

P

L

H

K

G

A

Q

P

G

-

S

I

W

F

D

Q

T

E

R

R

T

F

V

Y

L

I

S

T

S

P

I

K

R

R

R

-

D

-

Q

G

G

G

R

R

L

Y

L

V

L

I

G

G

S

A

L

-

G

-

R

-

V

-

D

T

N

M

K

K

P

P

A

H

W

T

F

F

V

N

R

K

-

T

-

V

-

Q

-

P

-

E

S

S

W

I

A

T

D

S

R

I

I

L

Q

E

S

R

H

A

Y

Y

-

T

-

I

Y

L

P

P

E

A

L

L

G

K

K

E

V

A

E

E

W

W

E

E

M

S

H

T

W

W

T

A

G
|
G

C

-

I

L

D
x
R

F
x
P

T

Q

P

S

D

N

H

H

-

E

-

A

-

P

L

Y

M

M

R

G

L

E

F

H

E

E

P

E

A

I

P

K

G

G

L

L

V

Y

A

A

V

C

T

T

G

G

Y

H

N
x
Y

G
x
R

R
x
N

G
|
G

N
x
I

T

L

G

S

T

P

V

I

I

S

G

G

R

Q

A

Y

F

M

A

A

E

D

F

L

L

I

binding flavin-adenine dinucleotide: I7 (= I38), G8 (= G39), G10 (= G41), V11 (≠ I42), I12 (≠ T43), V30 (≠ L61), E31 (= E62), K32 (≠ A63), E38 (≠ G69), A39 (≠ G70), S40 (= S71), A43 (≠ N74), G45 (= G76), L46 (= L77), V171 (= V212), G200 (≠ T240), G201 (= G241), W203 (≠ Y243), G298 (= G343), R300 (≠ D346), P301 (≠ F347), Y326 (≠ N369), R327 (≠ G370), N328 (≠ R371), G329 (= G372), I330 (≠ N373)

Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 49% coverage: 32:241/432 of query aligns to 42:242/857 of Q63342

query
sites
Q63342

K

R

A

A

D

E

V

T

C

V

V

I

I

I

G

G

I
x
C

T
x
V

G

G

L

V

S

S

A

L

A

A

I

Y

H

H

L

L

L

A

E

K

Q

A

G

G

-

M

K

R

S

D

V

V

I

V

V

L

L

L

E
|
E

A
x
K

W

S

K

E

I

L

G

T

H

A

G
|
G

G
x
S

S
x
T

G
x
W

R
x
H

N
x
A

V
x
A

G
|
G

L
|
L

V

T

N

-

A

-

G

-

T

T

W

Y

I

F

R

H

P

P

D

-

V

-

E

-

A

-

T

-

L

-

G

-

Q

-

K

-

Q

-

G

G

S

I

R

N

L

L

N

K

K

K

V

I

L

H

G

Y

E

D

A

S

P

I

A

K

E

L

V

Y

F

E

A

R

M

L

I

E

E

E

R

E

L

T

G

G

I

Q

D

V

C

V

Q

G

A

F

Q

H

H

Q

K

P

G

G

T

S

L

I

H

R

M

L

A

A

H

T

N

T

A

P

T

E

G

R

I

V

A

D

D

E

L

F

E

K

A

Y

R

Q

H

M

E

T

Q

R

W

T

R

N

R

W

R

H

G

A

A

T

D

E

V

Q

E

Y

L

I

T

E

G

P

A

E

Q

K

C

I

Q

H

E

E

-

L

-

F

-

P

Y

L

C

L

G

N

T

M

D

D

K

K

I

I

S

L

A

A

A

G

L

L

L

Y

D

N

R

P

R

G

A

D

G

G

T

H

I

I

N

D

P

P

M

Y

G

S

Y

L

T

T

Q

M

G

A

L

L

A

A

A

T

A

G

V

A

T

R

R

K

L

Y

G

G

G

V

K

L

I

L

F

K

Q

Y

Q

P

S

A

S

P

V
|
V

E

T

G

S

L

L

E

K

R

P

E

R

G

P

D

D

G

G

-

T

W

W

R

D

V

V

K

E

T

T

A

P

R

Q

G

G

A

S

V

V

R

R

A

A

E

N

K

R

V

I

V

V

I

N

S

A

T

A

G

G

CV 52:53 (≠ IT 42:43) binding
EK 73:74 (≠ EA 62:63) binding
80:88 (vs. 69:77, 33% identical) binding
H84 (≠ R73) modified: Tele-8alpha-FAD histidine
V212 (= V212) binding

Sites not aligning to the query:

244 binding
390:395 binding
573:575 binding
669 binding
676:678 binding
737 binding

4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
24% identity, 49% coverage: 32:241/432 of query aligns to 5:205/824 of 4pabB

query
sites
4pabB

K

R

A

A

D

E

V

T

C

V

V

I

I
|
I

G
|
G

G

G

G
|
G

I
x
C

T
x
V

G

G

L

V

S

S

A

L

A

A

I

Y

H

H

L

L

L

A

E

K

Q

A

G

G

-

M

K

R

S

D

V

V

I

V

V

L

L
|
L

E
|
E

A
x
K

W

S

K

E

I

L

G

T

H

A

G
|
G

G
x
S

S
x
T

G

W

R
x
H

N
x
A

V
x
A

G
|
G

L
|
L

V

T

N

-

A

-

G

-

T
|
T

W

Y

I

F

R

H

P

P

D

-

V

-

E

-

A

-

T

-

L

-

G

-

Q

-

K

-

Q

-

G

G

S

I

R

N

L

L

N

K

K

K

V

I

L

H

G

Y

E

D

A

S

P

I

A

K

E

L

V

Y

F

E

A

R

M

L

I

E

E

E

R

E

L

T

G

G

I

Q

D

V

C

V

Q

G

A

F

Q

H

H

Q

K

P

G

G

T

S

L

I

H

R

M

L

A

A

H

T

N

T

A

P

T

E

G

R

I

V

A

D

D
x
E

L

F

E

K

A

Y

R

Q

H

M

E

T

Q

R

W

T

R

N

R

W

R

H

G

A

A

T

D

E

V

Q

E

Y

L

I

T

E

G

P

A

E

Q

K

C

I

Q

H

E

E

-

L

-

F

-

P

Y

L

C

L

G

N

T

M

D

D

K

K

I

I

S

L

A

A

A

G

L

L

L

Y

D

N

R

P

R

G

A

D

G

G

T

H

I

I

N

D

P

P

M

Y

G

S

Y

L

T

T

Q

M

G

A

L

L

A

A

A

T

A

G

V

A

T

R

R

K

L

Y

G

G

G

V

K

L

I

L

F

K

Q

Y

Q

P

S

A

S

P

V
|
V

E

T

G

S

L

L

E

K

R

P

E

R

G

P

D

D

G

G

-

T

W

W

R

D

V

V

K

E

T

T

A

P

R

Q

G

G

A

S

V

V

R

R

A

A

E

N

K

R

V

I

V

V

I

N

S

A

T
x
A

G
|
G

active site: T53 (= T82), E102 (≠ D142)
binding flavin-adenine dinucleotide: I11 (= I38), G12 (= G39), G14 (= G41), C15 (≠ I42), V16 (≠ T43), L35 (= L61), E36 (= E62), K37 (≠ A63), G43 (= G69), S44 (≠ G70), T45 (≠ S71), H47 (≠ R73), A48 (≠ N74), A49 (≠ V75), G50 (= G76), L51 (= L77), V175 (= V212), A204 (≠ T240), G205 (= G241)

Sites not aligning to the query:

active site: 226, 255, 536
binding flavin-adenine dinucleotide: 207, 226, 228, 326, 328, 353, 355, 356, 357, 358
binding (6s)-5,6,7,8-tetrahydrofolate: 523, 536, 538, 550, 612, 613, 632, 639, 680, 700

2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
24% identity, 85% coverage: 34:400/432 of query aligns to 21:383/403 of 2gagB

query
sites
2gagB

D

D

V

A

C

I

V

I

I

V

G
|
G

G

G

G
|
G

I
x
G

T
x
H

G

G

L

L

S

A

A

T

A

A

I

Y

H

F

L

L

L

A

E

K

Q

N

G

H

-

G

-

I

K

T

S

N

V

V

I

A

V

V

L

L

E
|
E

A
x
K

W

G

K

W

I

L

G

A

H

G

G
|
G

G
x
N

S
x
M

G
x
A

R
|
R

N
|
N

V
x
T

G
x
T

L
x
I

V

I

N
x
R

A

S

G

N

-

Y

T

L

W

W

I

-

R

-

P

-

D

-

D

D

V

E

E

S

A

A

T

G

L

I

G

Y

Q

E

K

K

Q

S

G

-

S

-

R

-

L

-

N

L

K

K

V

L

L

W

G

E

E

Q

A

L

P

P

A

-

E

-

V

-

F

-

A

-

M

-

I

-

E

E

R

D

L

L

G

E

I

Y

D

D

C

F

Q

L

A

F

Q

S

H

Q

K

R

G

G

T

V

L

L

H

N

M

L

A

A

H

H

N

T

A

L

T

G

G

D

I

V

A

R

D

E

L

S

E

V

A

R

R

R

H

V

E

E

Q

A

W

N

R

K

R

L

R

N

G

G

A

V

D

D

V

A

E

E

L

W

L

L

T

D

G

P

A

S

Q

Q

C

V

Q

K

E

E

Y

A

C

C

-

P

-

I

-

N

G

T

T

S

D

D

K

D

I

I

S

R

A

Y

A

P

L

V

L

M

-

G

-

A

-

T

-

W

D

Q

R

P

R

R

A

A

G

G

T

I

I

A

N
x
K

P
x
H

M

D

G

H

Y

V

T

A

Q

W

G

A

L

F

A

A

A

R

A

K

V

A

T

N

R

E

L

M

G

G

G

V

K

D

I

I

F

I

Q

Q

Q

N

S

C

S

E

V
|
V

E

T

G

G

L

F

E

I

R

K

E

D

G

G

D

E

G

K

W

V

R

T

-

G

V

V

K

K

T

T

A

T

R

R

G

G

A

T

V

I

R

H

A

A

E

G

K

K

V

V

V

A

I

L

S

A

T
x
G

G
x
A

A

G

Y
x
H

T

S

E

-

G

-

D

-

W

-

S

S

N

V

L
|
L

Q

A

K

E

Q

-

F

-

F

M

R

A

G

G

Y

F

Y

E

Y

L

Q

P

V

I

A

Q

S

S

K

H

P

P

L
|
L

Q

Q

G

A

I

L

A
x
V

A

S

D

E

K

L

V

F

L

E

P

P

H

V

G

H

Q

P

G

T

S

V

W

V

D
x
M

T

S

R

N

T

H

V

I

L

H

S

V

S
x
Y

I

V

R

S

R

Q

D

A

D

H

Q
x
K

G

G

R
x
E

L

L

L

V

L

M

G

G

S

A

L

-

G

-

R

G

V

I

D

D

N

S

K

Y

P

N

A

G

W

Y

F

G

V

Q

R

R

S

G

W

A

A

F

D

H

R

V

I

I

Q

Q

S

E

H

Q

Y

M

-

A

-

V

-

E

-

L

Y

F

P

P

E

I

L

F

G

A

K

R

V

A

E

H

W

V

E

L

M
x
R

H

T

W
|
W

T

G

G

G

C

I

I

V

D

D

F

T

T

T

P

M

D

D

H

A

L

S

M

P

R

I

L

I

F

S

E

K

-

T

P

P

A

I

P

Q

G

N

L

L

V

Y

A

V

V

N

T

C

G

G

Y

W

N

-

G
|
G

R
x
T

G
|
G

N
x
G

T
x
F

T
x
K

G

G

T

T

V

P

-

G

I

A

G

G

R

F

A

T

F

L

A

A

E

H

F

T

L

I

L

A

K

N

G

D

E

E

A

P

D

H

S

E

L

L

P

N

I

K

P

P

F

F

S

S

active site: A61 (≠ G72), T64 (≠ V75), T65 (≠ G76)
binding flavin-adenine dinucleotide: G26 (= G39), G28 (= G41), G29 (≠ I42), H30 (≠ T43), E51 (= E62), K52 (≠ A63), G58 (= G69), N59 (≠ G70), M60 (≠ S71), R62 (= R73), N63 (= N74), T64 (≠ V75), I66 (≠ L77), V195 (= V212), G224 (≠ T240), A225 (≠ G241), H227 (≠ Y243), L231 (= L251), L246 (= L268), G352 (= G370), T353 (≠ R371), G354 (= G372), G355 (≠ N373), F356 (≠ T374), K357 (≠ T375)
binding flavin mononucleotide: A61 (≠ G72), R62 (= R73), H171 (≠ P188), V250 (≠ A272), E278 (≠ R300), R321 (≠ M339), W323 (= W341)
binding 2-furoic acid: T64 (≠ V75), I66 (≠ L77), R68 (≠ N79), M263 (≠ D285), Y270 (≠ S292), K357 (≠ T375)
binding sulfite ion: K170 (≠ N187), K276 (≠ Q298)

Sites not aligning to the query:

binding 2-furoic acid: 401

P40875 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 3 papers)
24% identity, 66% coverage: 117:400/432 of query aligns to 94:385/405 of P40875

query
sites
P40875

E

E

R

E

L

L

G

D

I

Y

D

D

C

F

Q

L

A

F

Q

S

H

Q

K

R

G

G

T

V

L

L

H

N

M

L

A

A

H

H

N

T

A

L

T

G

G

D

I

V

A

R

D

E

L

S

E

V

A

R

R

R

H

V

E

E

Q

A

W

N

R

K

R

F

R

N

G

G

A

V

D

D

V

A

E

E

L

W

L

L

T

T

G

P

A

E

Q

Q

C

V

Q

K

E

E

Y

V

C
|
C

-

P

-

I

-

N

-

I

G

G

T

D

D

D

-

I

-

R

-

Y

K

P

I

V

S

M

A

G

A

A

L

T

L

Y

D

Q

R

P

R

R

A

A

G

G

T

I

I

A

N

K

P
x
H

M

D

G
x
H

Y

V

T

A

Q

W

G

A

L

F

A

A

A

R

A

K

V

A

T

N

R

E

L

M

G

G

G

V

K

D

I

I

F

I

Q

Q

Q

N

S
x
C

S

E

V

V

E

T

G

G

L

F

E

L

R

K

E

D

G

G

D

E

G

K

W

V

R

T

-

G

V

V

K

K

T

T

A

T

R

R

G

G

A

T

V

I

R

H

A

A

E

G

K

K

V

V

V

A

I

L

S

A

T

G

G

A

A

G

Y

H

T

S

E

-

G

-

D

-

W

-

S

S

N

V

L

L

Q

A

K

E

Q

-

F

-

F

L

R

A

G

G

Y

F

Y

E

Y

L

Q

P

V

I

A

Q

S

S

K

H

P

P

L

L

Q

Q

G

A

I

L

A

V

A

S

D

E

K

L

V

F

L

E

P

P

H

V

G

H

Q

P

G

T

S

V

W

V

D

M

T

S

R

N

T

H

V

I

L

H

S

V

S

Y

I

V

R

S

R

Q

D

A

D

H

Q

K

G

G

R

E

L

L

L

V

L

M

G

G

-

A

-

G

-

I

-

D

S

S

L

Y

G

N

R

G

V

Y

D

G

N

Q

K

R

P

G

A

A

W

F

F

H

V

V

R

I

S

E

W

E

A

Q

D

M

R

A

I

A

Q

A

S

V

H

E

Y

L

Y

F

P

P

E

I

L

F

G

A

K

R

V

A

E

H

W

V

E

L

M

R

H

T

W

W

T

G

G

G

C

I

I

V

D

D

F

T

T

T

P

M

D

D

H

A

L

S

M

P

R

I

L

I

F

S

E

K

-

T

P

P

A

I

P

Q

G

N

L

L

V

Y

A

V

V

N

T
x
C

G

G

Y

W

N

-

G

G

R

T

G

G

N

G

T

F

T

K

G

G

T

T

V

P

-

G

I

A

G

G

R

F

A

T

F

L

A

A

E

H

F

T

L

I

L

A

K

N

G

D

E

E

A

A

D

H

S

A

L

L

P

N

I

A

P

P

F

F

S

S

C146 (= C169) mutation to S: No change in activity.
H173 (≠ P188) modified: Tele-8alpha-FMN histidine; mutation to N: Prevents covalent attachment of FMN. Loss of activity. The mutant is considerably less stable than wild-type enzyme, the beta and delta subunits are lost during purification, which yields a stable alpha-gamma complex.
H175 (≠ G190) mutation to A: No effect on FMN binding and activity.
C195 (≠ S210) mutation to S: No change in activity.
C351 (≠ T366) mutation to A: No change in activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
30 G→A: Prevents covalent attachment of FMN. Blocks subunit assembly.

1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
23% identity, 65% coverage: 122:400/432 of query aligns to 98:384/402 of 1vrqB

query
sites
1vrqB

D

D

C

F

Q

L

A

F

Q

S

H

Q

K

R

G

G

T

V

L

L

H

N

M

L

A

A

H

H

N

T

A

L

T

G

G

D

I

V

A

R

D

E

L

S

E

I

A

R

R

R

H

V

E

E

Q

A

W

N

R

K

R

F

R

N

G

G

A

V

D

D

V

A

E

E

L

W

L

L

T

T

G

P

A

E

Q

Q

C

V

Q

K

E

E

Y

V

C

C

G

P

T

I

-

I

-

N

-

T

-

G

D

D

K

N

I

I

S

R

-

Y

-

P

-

V

-

M

A

G

A

A

L

T

L

Y

D

Q

R

P

R

R

A

A

G

G

T

I

I

A

N

K

P
x
H

M

D

G

H

Y

V

T

A

Q

W

G

A

L

F

A

A

A

R

A

K

V

A

T

N

R

E

L

M

G

G

G

V

K

D

I

I

F

I

Q

Q

Q

N

S

C

S

E

V
|
V

E

T

G

G

L

F

E

L

R

K

E

D

G

G

D

E

G

K

W

V

R

T

-

G

V

V

K

K

T

T

A

T

R

R

G

G

A

T

V

I

R

L

A

A

E

G

K

K

V

V

V

A

I

L

S
x
A

T
x
G

G

A

A

G

Y
x
H

T

S

E

-

G

-

D

-

W

-

S

S

N

V

L

L

Q

A

K

E

Q

-

F

-

F

L

R

A

G

G

Y

F

Y

E

Y

L

Q

P

V

I

A

Q

S

S

K

H

P

P

L
|
L

Q

Q

G

A

I

L

A
x
V

A

S

D

E

K

L

V

F

L

E

P

P

H

V

G

H

Q

P

G

T

S

V

W

V

D

M

T

S

R

N

T

H

V

I

L

H

S

V

S

Y

I

V

R

S

R

Q

D

A

D

H

Q

K

G

G

R
x
E

L

L

L

V

L

M

G

G

-

A

-

G

-

I

-

D

S

S

L

Y

G

N

R

G

V

Y

D

G

N

Q

K

R

P

G

A

A

W

F

F

H

V

V

R

I

S

E

W

E

A

Q

D

M

R

A

I

A

Q

A

S

V

H

E

Y

L

Y

F

P

P

E

I

L

F

G

A

K

R

V

A

E

H

W

V

E

L

M
x
R

H

T

W
|
W

T

G

G
|
G

C

I

I

V

D

D

F

T

T

T

P

M

D

D

H

A

L

S

M

P

R

I

L

I

F

S

E

K

-

T

P

P

A

I

P

Q

G

N

L

L

V

Y

A

V

V

N

T

C

G

G

Y

W

N

-

G
|
G

R
x
T

G
|
G

N
x
G

T
x
F

T
x
K

G

G

T

T

V

P

-

G

I

A

G

G

R

Y

A

T

F

L

A

A

E

H

F

T

L

I

L

A

K

H

G

D

E

E

A

P

D

H

S

K

L

L

P

N

I

A

P

P

F

F

S

A

active site: G326 (= G343), K358 (≠ T375)
binding n,n-dimethylglycine: K358 (≠ T375)
binding flavin-adenine dinucleotide: V196 (= V212), A224 (≠ S239), G225 (≠ T240), H228 (≠ Y243), L247 (= L268), G353 (= G370), T354 (≠ R371), G355 (= G372), G356 (≠ N373), F357 (≠ T374), K358 (≠ T375)
binding flavin mononucleotide: H172 (≠ P188), V251 (≠ A272), E279 (≠ R300), R322 (≠ M339), W324 (= W341)

Sites not aligning to the query:

active site: 62, 65, 66
binding n,n-dimethylglycine: 65, 67, 69, 401
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 51, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63

3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
23% identity, 65% coverage: 122:400/432 of query aligns to 98:384/404 of 3ad8B

query
sites
3ad8B

D

D

C

F

Q

L

A

F

Q

S

H

Q

K

R

G

G

T

V

L

L

H

N

M

L

A

A

H

H

N

T

A

L

T

G

G

D

I

V

A

R

D

E

L

S

E

I

A

R

R

R

H

V

E

E

Q

A

W

N

R

K

R

F

R

N

G

G

A

V

D

D

V

A

E

E

L

W

L

L

T

T

G

P

A

E

Q

Q

C

V

Q

K

E

E

Y

V

C

C

G

P

T

I

-

I

-

N

-

T

-

G

D

D

K

N

I

I

S

R

-

Y

-

P

-

V

-

M

A

G

A

A

L

T

L

Y

D

Q

R

P

R

R

A

A

G

G

T

I

I

A

N

K

P
x
H

M

D

G

H

Y

V

T

A

Q

W

G

A

L

F

A

A

A

R

A

K

V

A

T

N

R

E

L

M

G

G

G

V

K

D

I

I

F

I

Q

Q

Q

N

S

C

S

E

V
|
V

E

T

G

G

L

F

E

L

R

K

E

D

G

G

D

E

G

K

W

V

R

T

-

G

V

V

K

K

T

T

A

T

R

R

G

G

A

T

V

I

R

L

A

A

E

G

K

K

V

V

V

A

I

L

S

A

T
x
G

G
x
A

A

G

Y
x
H

T

S

E

-

G

-

D

-

W

-

S

S

N

V

L

L

Q

A

K

E

Q

-

F

-

F

L

R

A

G

G

Y

F

Y

E

Y

L

Q

P

V

I

A

Q

S

S

K

H

P

P

L
|
L

Q

Q

G

A

I

L

A
x
V

A

S

D

E

K

L

V

F

L

E

P

P

H

V

G

H

Q

P

G

T

S

V

W

V

D
x
M

T

S

R

N

T

H

V

I

L

H

S

V

S
x
Y

I

V

R

S

R

Q

D

A

D

H

Q

K

G

G

R
x
E

L

L

L

V

L

M

G

G

-

A

-

G

-

I

-

D

S

S

L

Y

G

N

R

G

V

Y

D

G

N

Q

K

R

P

G

A

A

W

F

F

H

V

V

R

I

S

E

W

E

A

Q

D

M

R

A

I

A

Q

A

S

V

H

E

Y

L

Y

F

P

P

E

I

L

F

G

A

K

R

V

A

E

H

W

V

E

L

M
x
R

H

T

W
|
W

T

G

G
|
G

C

I

I

V

D

D

F

T

T

T

P

M

D

D

H

A

L

S

M

P

R

I

L

I

F

S

E

K

-

T

P

P

A

I

P

Q

G

N

L

L

V

Y

A

V

V

N

T

C

G

G

Y

W

N

-

G
|
G

R
x
T

G
|
G

N
x
G

T
x
F

T
x
K

G

G

T

T

V

P

-

G

I

A

G

G

R

Y

A

T

F

L

A

A

E

H

F

T

L

I

L

A

K

H

G

D

E

E

A

P

D

H

S

K

L

L

P

N

I

A

P

P

F

F

S

A

active site: G326 (= G343), K358 (≠ T375)
binding flavin-adenine dinucleotide: V196 (= V212), G225 (≠ T240), A226 (≠ G241), H228 (≠ Y243), L247 (= L268), G353 (= G370), T354 (≠ R371), G355 (= G372), G356 (≠ N373), F357 (≠ T374), K358 (≠ T375)
binding flavin mononucleotide: H172 (≠ P188), V251 (≠ A272), E279 (≠ R300), R322 (≠ M339), W324 (= W341)
binding pyrrole-2-carboxylate: M264 (≠ D285), Y271 (≠ S292), T354 (≠ R371), K358 (≠ T375)

Sites not aligning to the query:

active site: 62, 65, 66
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63
binding pyrrole-2-carboxylate: 65, 67, 69, 402

3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
23% identity, 65% coverage: 122:400/432 of query aligns to 98:384/404 of 3ad7B

query
sites
3ad7B

D

D

C

F

Q

L

A

F

Q

S

H

Q

K

R

G

G

T

V

L

L

H

N

M

L

A

A

H

H

N

T

A

L

T

G

G

D

I

V

A

R

D

E

L

S

E

I

A

R

R

R

H

V

E

E

Q

A

W

N

R

K

R

F

R

N

G

G

A

V

D

D

V

A

E

E

L

W

L

L

T

T

G

P

A

E

Q

Q

C

V

Q

K

E

E

Y

V

C

C

G

P

T

I

-

I

-

N

-

T

-

G

D

D

K

N

I

I

S

R

-

Y

-

P

-

V

-

M

A

G

A

A

L

T

L

Y

D

Q

R

P

R

R

A

A

G

G

T

I

I

A

N

K

P
x
H

M

D

G

H

Y

V

T

A

Q

W

G

A

L

F

A

A

A

R

A

K

V

A

T

N

R

E

L

M

G

G

G

V

K

D

I

I

F

I

Q

Q

Q

N

S

C

S

E

V
|
V

E

T

G

G

L

F

E

L

R

K

E

D

G

G

D

E

G

K

W

V

R

T

-

G

V

V

K

K

T

T

A

T

R

R

G

G

A

T

V

I

R

L

A

A

E

G

K

K

V

V

V

A

I

L

S

A

T
x
G

G
x
A

A

G

Y
x
H

T

S

E

-

G

-

D

-

W

-

S

S

N

V

L

L

Q

A

K

E

Q

-

F

-

F

L

R

A

G

G

Y

F

Y

E

Y

L

Q

P

V

I

A

Q

S

S

K

H

P

P

L
|
L

Q

Q

G

A

I

L

A
x
V

A

S

D

E

K

L

V

F

L

E

P

P

H

V

G

H

Q

P

G

T

S

V

W

V

D
x
M

T

S

R

N

T

H

V

I

L

H

S

V

S
x
Y

I

V

R

S

R

Q

D

A

D

H

Q
x
K

G

G

R
x
E

L

L

L

V

L

M

G

G

-

A

-

G

-

I

-

D

S

S

L

Y

G

N

R

G

V

Y

D

G

N

Q

K

R

P

G

A

A

W

F

F

H

V

V

R

I

S

E

W

E

A

Q

D

M

R

A

I

A

Q

A

S

V

H

E

Y

L

Y

F

P

P

E

I

L

F

G

A

K

R

V

A

E

H

W

V

E

L

M
x
R

H

T

W
|
W

T

G

G
|
G

C

I

I

V

D

D

F

T

T

T

P

M

D

D

H

A

L

S

M

P

R

I

L

I

F

S

E

K

-

T

P

P

A

I

P

Q

G

N

L

L

V

Y

A

V

V

N

T

C

G

G

Y

W

N

-

G
|
G

R
x
T

G
|
G

N
x
G

T
x
F

T
x
K

G

G

T

T

V

P

-

G

I

A

G

G

R

Y

A

T

F

L

A

A

E

H

F

T

L

I

L

A

K

H

G

D

E

E

A

P

D

H

S

K

L

L

P

N

I

A

P

P

F

F

S

A

active site: G326 (= G343), K358 (≠ T375)
binding flavin-adenine dinucleotide: V196 (= V212), G225 (≠ T240), A226 (≠ G241), H228 (≠ Y243), L247 (= L268), G353 (= G370), T354 (≠ R371), G355 (= G372), G356 (≠ N373), F357 (≠ T374), K358 (≠ T375)
binding flavin mononucleotide: H172 (≠ P188), V251 (≠ A272), K277 (≠ Q298), E279 (≠ R300), R322 (≠ M339), W324 (= W341)
binding [methylthio]acetate: M264 (≠ D285), Y271 (≠ S292), T354 (≠ R371), K358 (≠ T375)

Sites not aligning to the query:

active site: 62, 65, 66
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63
binding [methylthio]acetate: 67, 69, 402

Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
23% identity, 65% coverage: 122:400/432 of query aligns to 99:385/405 of Q50LF2

query
sites
Q50LF2

D

D

C

F

Q

L

A

F

Q

S

H

Q

K

R

G

G

T

V

L

L

H

N

M

L

A

A

H

H

N

T

A

L

T

G

G

D

I

V

A

R

D

E

L

S

E

I

A

R

R

R

H

V

E

E

Q

A

W

N

R

K

R

F

R

N

G

G

A

V

D

D

V

A

E

E

L

W

L

L

T

T

G

P

A

E

Q

Q

C

V

Q

K

E

E

Y

V

C

C

G

P

T

I

-

I

-

N

-

T

-

G

D

D

K

N

I

I

S

R

-

Y

-

P

-

V

-

M

A

G

A

A

L

T

L

Y

D

Q

R

P

R

R

A

A

G

G

T

I

I

A

N
x
K

P
x
H

M

D

G

H

Y

V

T

A

Q

W

G

A

L

F

A

A

A

R

A

K

V

A

T

N

R

E

L

M

G

G

G

V

K

D

I

I

F

I

Q

Q

Q

N

S

C

S

E

V
|
V

E

T

G

G

L

F

E

L

R

K

E

D

G

G

D

E

G

K

W

V

R

T

-

G

V

V

K

K

T

T

A

T

R

R

G

G

A

T

V

I

R

L

A

A

E

G

K

K

V

V

V

A

I

L

S

A

T

G

G

A

A

G

Y

H

T

S

E

-

G

-

D

-

W

-

S

S

N

V

L

L

Q

A

K

E

Q

-

F

-

F

L

R

A

G

G

Y

F

Y

E

Y

L

Q

P

V

I

A

Q

S

S

K

H

P

P

L

L

Q

Q

G

A

I

L

A

V

A

S

D

E

K

L

V

F

L

E

P

P

H

V

G

H

Q

P

G

T

S

V

W

V

D

M

T

S

R

N

T

H

V

I

L
x
H

S

V

S
x
Y

I

V

R

S

R

Q

D

A

D

H

Q

K

G

G

R

E

L

L

L

V

L

M

G

G

-

A

-

G

-

I

-

D

S

S

L

Y

G

N

R

G

V

Y

D

G

N

Q

K

R

P

G

A

A

W

F

F

H

V

V

R

I

S

E

W

E

A

Q

D

M

R

A

I

A

Q

A

S

V

H

E

Y

L

Y

F

P

P

E

I

L

F

G

A

K

R

V

A

E

H

W

V

E

L

M

R

H

T

W

W

T

G

G

G

C

I

I

V

D

D

F

T

T

T

P

M

D

D

H

A

L

S

M

P

R

I

L

I

F

S

E

K

-

T

P

P

A

I

P

Q

G

N

L

L

V

Y

A

V

V

N

T

C

G

G

Y

W

N

-

G
|
G

R

T

G

G

N
x
G

T

F

T
x
K

G

G

T

T

V

P

-

G

I

A

G

G

R

Y

A

T

F

L

A

A

E

H

F

T

L

I

L

A

K

H

G

D

E

E

A

P

D

H

S

K

L

L

P

N

I

A

P

P

F

F

S

A

K172 (≠ N187) mutation to A: Retains 39% of wild-type activity.; mutation to D: Retains 32% of wild-type activity.; mutation to R: Retains 58% of wild-type activity.
H173 (≠ P188) modified: Tele-8alpha-FMN histidine
V197 (= V212) binding
H270 (≠ L290) mutation to A: 10-fold decrease in catalytic efficiency.
Y272 (≠ S292) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
G354 (= G370) binding
G357 (≠ N373) binding
K359 (≠ T375) binding ; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.

Sites not aligning to the query:

31 binding
32 binding
53 binding
61 binding
62 binding
66 binding
68 binding

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
22% identity, 51% coverage: 50:270/432 of query aligns to 22:224/364 of 3if9A

query
sites
3if9A

H

Y

L

L

L

A

E

K

Q

E

G

N

K

K

S

N

V

T

I

A

V

L

L

F

E
|
E

A
x
S

W

G

K

T

I

M

G

G

H

G

G

R

G
x
T

S
x
T

G

S

R

A

N
x
A

V
x
A

G
|
G

L
x
M

V

L

N

S

A

A

G

H

T

R

W

E

I

C

R

E

P

E

D

R

D

D

V

A

E

F

A

F

T

D

L

F

G

A

Q

M

K

-

Q

H

G

S

S

Q

R

R

L

L

N

Y

K

K

V

G

L

L

G

G

E

E

A

-

P

-

A

-

E

E

V

L

F

Y

A

A

M

L

I

S

E

-

R

-

L

-

G

G

I

V

D

D

C

I

Q

R

A

Q

Q

H

H

N

K

G

G

G

T

M

L

F

H

K

M

L

A

A

H

F

N

S

A

E

T

E

G

D

I

V

A

L

D

Q

L

L

E

-

A

-

R

-

H

-

E

-

Q

-

W

-

R

-

R

R

R

Q

G

M

A

D

D

D

V

L

E

D

L

S

L

V

T

S

G

W

A

Y

Q

S

C

K

Q

E

E

E

-

V

-

L

-

E

-

K

-

E

-

P

Y

Y

C

A

G

S

T

G

D

D

K

I

I

F

S

G

A

A

A

S

L

F

L

I

D

Q

R

D

A

V

G

-

T

H

I

V

N

E

P

P

M

Y

G

F

Y

V

T

C

Q

K

G

A

L

Y

A

V

A

K

A

A

V

A

T

K

R

M

L

L

G

G

G

A

K

E

I

I

F

F

Q

E

Q

H

S

T

S
x
P

V
|
V

E

L

G

H

L

V

E

E

R

R

E

D

G

G

D

E

G

A

W

L

R

F

V

I

K

K

T

T

A

P

R

S

G

G

A

D

V

V

R

W

A

A

E

N

K

H

V

V

I

V

S

A

T
x
S

G
|
G

A

V

Y

-

T

-

E

-

G

-

D

-

W
|
W

S

S

N

G

L

M

Q

-

K

-

Q

-

F
|
F

F

F

R

K

G

Q

Y

L

Y

G

Y

L

Q

N

V

N

A

A

S

F

K

L

P

P

L

V

Q

K

G

G

active site: A47 (≠ V75), G48 (= G76), M49 (≠ L77)
binding flavin-adenine dinucleotide: E34 (= E62), S35 (≠ A63), T42 (≠ G70), T43 (≠ S71), A46 (≠ N74), A47 (≠ V75), G48 (= G76), M49 (≠ L77), P173 (≠ S211), V174 (= V212), S202 (≠ T240), G203 (= G241), W205 (= W248), F209 (= F255)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 15, 300, 302, 327, 328, 329, 330, 331, 332
binding glycolic acid: 246, 302, 329

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
22% identity, 51% coverage: 50:270/432 of query aligns to 22:224/364 of 1ng3A

query
sites
1ng3A

H

Y

L

L

L

A

E

K

Q

E

G

N

K

K

S

N

V

T

I

A

V

L

L
x
F

E
|
E

A
x
S

W

G

K

T

I

M

G

G

H

G

G
x
R

G
x
T

S
x
T

G

S

R

A

N
x
A

V
x
A

G
|
G

L
x
M

V

L

N

G

A

A

G

H

T

A

W

E

I

C

R

E

P

E

D

R

D

D

V

A

E

F

A

F

T

D

L

F

G

A

Q

M

K

-

Q

H

G

S

S

Q

R

R

L

L

N

Y

K

K

V

G

L

L

G

G

E

E

A

-

P

-

A

-

E

E

V

L

F

Y

A

A

M

L

I

S

E

-

R

-

L

-

G

G

I

V

D

D

C

I

Q
x
R

A

Q

Q

H

H

N

K

G

G

G

T

M

L

F

H

K

M

L

A

A

H

F

N

S

A

E

T

E

G

D

I

V

A

L

D

Q

L

L

E

-

A

-

R

-

H

-

E

-

Q

-

W

-

R

-

R

R

R

Q

G

M

A

D

D

D

V

L

E

D

L

S

L

V

T

S

G

W

A

Y

Q

S

C

K

Q

E

E

E

-

V

-

L

-

E

-

K

-

E

-

P

Y

Y

C

A

G

S

T

G

D

D

K

I

I

F

S

G

A

A

A

S

L

F

L

I

D

Q

R

D

A

V

G

-

T

H

I

V

N

E

P

P

M

Y

G

F

Y

V

T

C

Q

K

G

A

L

Y

A

V

A

K

A

A

V

A

T

K

R

M

L

L

G

G

G

A

K

E

I

I

F

F

Q

E

Q

H

S

T

S

P

V
|
V

E

L

G

H

L

V

E

E

R

R

E

D

G

G

D

E

G

A

W

L

R

F

V

I

K

K

T

T

A

P

R

S

G

G

A

D

V

V

R

W

A

A

E

N

K

H

V

V

I

V

S

A

T
x
S

G
|
G

A

V

Y

-

T

-

E

-

G

-

D

-

W
|
W

S

S

N

G

L

M

Q

-

K

-

Q

-

F
|
F

F

F

R

K

G

Q

Y

L

Y

G

Y

L

Q

N

V

N

A

A

S

F

K

L

P

P

L

V

Q

K

G

G

active site: A47 (≠ V75), G48 (= G76), M49 (≠ L77)
binding flavin-adenine dinucleotide: F33 (≠ L61), E34 (= E62), S35 (≠ A63), R41 (≠ G69), T42 (≠ G70), T43 (≠ S71), A46 (≠ N74), A47 (≠ V75), G48 (= G76), M49 (≠ L77), V174 (= V212), S202 (≠ T240), G203 (= G241), W205 (= W248), F209 (= F255)
binding phosphate ion: R89 (≠ Q124)

Sites not aligning to the query:

binding acetylamino-acetic acid: 246, 302, 329
binding flavin-adenine dinucleotide: 11, 13, 15, 300, 302, 327, 329, 330, 331, 332
binding phosphate ion: 254

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
22% identity, 51% coverage: 50:270/432 of query aligns to 22:224/369 of O31616

query
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O31616

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ES 34:35 (≠ EA 62:63) binding
TT 42:43 (≠ GS 70:71) binding
AGM 47:49 (≠ VGL 75:77) binding
G51 (≠ N79) mutation to R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; mutation to S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
A54 (≠ T82) mutation to R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
V174 (= V212) binding

Sites not aligning to the query:

14:15 binding
244 H→A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
302 binding
327:333 binding
329 binding

Query Sequence

>PP_5257 FitnessBrowser__Putida:PP_5257
MEPAMSELRQQCLWEHVSKPTVAAQALAGEHKADVCVIGGGITGLSAAIHLLEQGKSVIV
LEAWKIGHGGSGRNVGLVNAGTWIRPDDVEATLGQKQGSRLNKVLGEAPAEVFAMIERLG
IDCQAQHKGTLHMAHNATGIADLEARHEQWRRRGADVELLTGAQCQEYCGTDKISAALLD
RRAGTINPMGYTQGLAAAVTRLGGKIFQQSSVEGLEREGDGWRVKTARGAVRAEKVVIST
GAYTEGDWSNLQKQFFRGYYYQVASKPLQGIAADKVLPHGQGSWDTRTVLSSIRRDDQGR
LLLGSLGRVDNKPAWFVRSWADRIQSHYYPELGKVEWEMHWTGCIDFTPDHLMRLFEPAP
GLVAVTGYNGRGNTTGTVIGRAFAEFLLKGEADSLPIPFSPMSGVSAPSLRTAFYESGFS
LYHAGQCLRVVL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory