SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_4413 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4413 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
32% identity, 88% coverage: 11:405/451 of query aligns to 15:420/425 of O59010

query
sites
O59010

Q

K

V

I

L

L

I

I

A

G

V

L

I

I

L

L

G

G

A

A

I

I

V

V

G

G

V

L

F

I

V

L

P

G

E

H

T

Y

G

G

-

Y

-

A

-

D

-

A

-

V

-

K

T

T

A

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

F

L

A

T
x
S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M

I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L

V

I

V

Y

Y

F

Y

E

L

V

L

V

T

S

S

T

A

L

F

A

A

L

V

V

T

V

L

G

G

L

I

V

I

V

M

V

A

D

R

V

L

F

F

K

N

P

P

G

G

A

A

G

G

M

I

N

H

I

L

D

A

V

V

A

G

S

G

L

Q

D

Q

T

F

S

Q

S

P

L

-

A

-

T

-

Y

-

T

-

A

-

V

-

K

K

H

Q

A

A

S

P

-

P

F

L

M

V

D

K

F

I

V

L

M

L

N

D

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

G

A

A

F

L

A

A

K

N

G

G

N

Q

V

V

L

L

Q

P

I

T

L

I

L

F

F

F

S

A

I

I

L

I

L

L

G

G

V

I

A

A

L

I

A

T

H

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

P

K

R

S

A

A

K

E

V

T

F

L

V

L

D

D

T

A

L

I

D

N

S

G

L

L

M

A

Q

E

G

A

M

M

F

Y

R

K

I

I

V

V

N

N

M

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

K

S

V

L

V

F

G

S

E

L

L

G

A

K

K

L

V

M

T

A

A

C

A

V

V

Y

Y

L

V

T

G

C

L

A

T

V

L

F

Q

V

I

I

L

F

L

V

V

L

Y

G

F

P

V

I

L

C

L

R

K

Y

I

S

Y

G

G

F

I

S

D

L

P

W

I

K

S

F

F

L

I

K

K

F

K

I

A

K

K

E

D

E

A

L

M

F

L

T

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

Q

V

M

T

I

M

S

R

K

V

M

A

E

K

K

E

A

M

G

G

V

I

S

S

K

E

P

G

V

I

A

Y

G

S

M

F

I

T

I

L

P

P

S

L

G

G

L

A

T

T

F

I

N

N

P
x
M

D

D

G

G

Q
x
T

A

A

I

L

Y

Y

Y

Q

T

G

I

V

A

C

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

T

S

P

H

L

L

T

T

L

V

T

G

D

Q

Q

Q

L

L

I

T

V

I

L

V

A

L

V

T

L

A

M

V

F

L

T

A

S

S

K

I

G

G

S

T

A

A

G

G

V
|
V

T

P

G

G

S

A

G

G

F

A

I

I

I

M

L

L

A

A

A

M

T

V

L

L

S

E

S

S

L

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

G

A

M

Y

V

A

L

M

L

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E
x
M

A

G

R
|
R

A

T

I

M

T

V

N
|
N

T

V

I

T

G

G

N
x
D

G

L

V

T

G

G

T

T

M

A

A

I

I

V

A

A

K

K

W

T

V

E

G

G

A

E

L

L

D

E

S65 (≠ T55) mutation to V: Strongly decreased chloride conductance.
R276 (≠ S266) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ SSS 266:268) binding
M311 (≠ P301) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (≠ Q304) binding
V355 (= V345) binding
D394 (≠ S379) binding
M395 (≠ E380) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R382) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N386) binding
D405 (≠ N390) mutation to N: Strongly decreased affinity for aspartate.

2nwwA Crystal structure of gltph in complex with tboa (see paper)
32% identity, 86% coverage: 11:399/451 of query aligns to 6:405/407 of 2nwwA

query
sites
2nwwA

Q

K

V

I

L

L

I

I

A

G

V

L

I

I

L

L

G

G

A

A

I

I

V

V

G

G

V

L

F

I

V

L

P

G

E

H

T

Y

G

G

-

Y

-

A

-

H

-

A

-

V

-

H

T

T

A

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M

I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L

V

I

V

Y

Y

F

Y

E

L

V

L

V

T

S

S

T

A

L

F

A

A

L

V

V

T

V

L

G

G

L

I

V

I

V

M

V

A

D

R

V

L

F

F

K

N

P

P

G

G

A

A

G

G

M

I

N

H

I

L

D

A

V

V

A

G

S

G

L

Q

D

Q

T

F

S

Q

S

P

L

H

A

-

T

-

Y

-

T

-

A

-

V

-

K

Q

H

A

A

P

S

P

F

L

M

V

D

H

F

I

V

L

M

L

N

D

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

G

A

A

F

L

A

A

K

N

G

G

N

Q

V

V

L

L

Q

P

I

T

L

I

L

F

F

F

S

A

I

I

L

I

L

L

G

G

V

I

A

A

L

I

A

T

H

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

P

K

R

S

A

A

K

E

V

T

F

L

V

L

D

D

T

A

L

I

D

N

S

G

L

L

M

A

Q

E

G

A

M

M

F

Y

R

K

I

I

V

V

N

N

M

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

H

S

V

L

V

F

G

S

E

L

L

G

A

K

K

L

V

M

T

A

A

C

A

V

V

Y

Y

L

V

T

G

C

L

A

T

V

L

F

Q

V

I

I

L

F

L

V

V

L

Y

G

F

P

V

I

L

C

L

R

K

Y

I

S

Y

G

G

F

I

S

D

L

P

W

I

K

S

F

F

L

I

K

K

F

H

I

A

K

K

E

D

E

A

L

M

F

L

T

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

Q

V

M

T

I

M

S

R

K

V

M

A

E

K

K

E

A

M

G

G

V

I

S

S

K

E

P

G

V

I

A

Y

G

S

M

F

I

T

I

L

P

P

S

L

G

G

L

A

T

T

F

I

N

N

P

M

D

D

G

G

Q
x
T

A

A

I

L

Y

Y

Y

Q

T

G

I

V

A

C

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

T

S

P

H

L

L

T

T

L

V

T

G

D

Q

Q

Q

L

L

I

T

V

I

L

V

A

L

V

T

L

A

M

V

F

L

T

A

S

S

K

I

G

G

S

T

A

A

G

G

V

V

T

P

G
|
G

S
x
A

G
|
G

F

A

I

I

I

M

L

L

A

A

A

M

T

V

L

L

S

H

S

S

L

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

G

A

M

Y

V

A

L

M

L

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

I

M

T

V

N
|
N

T

V

I

T

G

G

N

D

G

L

V

T

G

G

T

T

M

A

A

I

I

V

A

A

K

K

binding (3s)-3-(benzyloxy)-l-aspartic acid: R267 (≠ S266), S268 (= S267), S269 (= S268), T305 (≠ Q304), G348 (= G347), A349 (≠ S348), G350 (= G349), D385 (≠ S379), R388 (= R382), T389 (≠ A383), N392 (= N386)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
32% identity, 87% coverage: 11:404/451 of query aligns to 15:419/419 of 6x15A

query
sites
6x15A

Q
x
K

V

I

L

L

I
|
I

A

G

V

L

I

I

L

L

G

G

A

A

I

I

V

V

G

G

V

L

F

I

V

L

P

G

E

H

T

Y

G

G

-

Y

-

A

-

H

-

A

-

V

-

H

T

T

A
x
Y

L

V

K

K

P
|
P

L
x
F

G

G

D

D

A
x
L

F

F

I

V

K

R

L
|
L

I

L

K

C

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M
x
I

E

S

N
x
P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R
x
K

A

I

L

V

I

V

Y

Y

F
x
Y

E
x
L

V
x
L

V
x
T

S
|
S

T

A

L
x
F

A

A

L

V

V

T

V

L

G

G

L

I

V

I

V

M

V

A

D

R

V

L

F

F

K

N

P

P

G

G

A

A

G

G

M

I

N

H

I

L

D

A

V

V

A

G

S

G

L

Q

D

Q

T

F

S

Q

S

P

L

H

A

-

T

-

Y

-

T

-

A

-

V

-

K

Q

H

A

A

P

S

P

F
x
L

M
x
V

D

H

F
x
I

V
x
L

M

L

N

D

I
|
I

I

V

P

P

D

T

T

N

I

P

V

F

D

G

A

A

F

L

A

A

K

N

G

G

N

Q

V

V

L
|
L

Q

P

I

T

L

I

L
x
F

F

F

S

A

I
|
I

L

I

L

L

G

G

V
x
I

A
|
A

L

I

A

T

H
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

P

K

R

S

A

A

K

E

V

T

F

L

V

L

D

D

T

A

L

I

D

N

S

G

L

L

M

A

Q

E

G

A

M

M

F

Y

R
x
K

I
|
I

V

V

N
|
N

M
x
G

V

V

M

M

R

Q

L
x
Y

A

A

P

P

I
|
I

G

G

A

V

F
|
F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

H

S

V

L

V

F

G

S

E

L

L

G

A

K

K

L

V

M

T

A

A

C

A

V

V

Y

Y

L

V

T

G

C

L

A

T

V

L

F

Q

V

I

I

L

F

L

V

V

L

Y

G

F

P

V

I
x
L

C

L

R

K

Y
x
I

S
x
Y

G

G

F

I

S

D

L

P

W

I

K

S

F

F

L
x
I

K

K

F

H

I

A

K
|
K

E

D

E

A

L
x
M

F

L

T

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

Q

V

M

T

I

M

S

R

K

V

M

A

E

K

K

E

A

M

G

G

V

I

S

S

K

E

P

G

V

I

A

Y

G

S

M

F

I

T

I

L

P

P

S

L

G
|
G

L
x
A

T
|
T

F

I

N
|
N

P
x
M

D
|
D

G

G

Q
x
T

A

A

I

L

Y

Y

Y

Q

T

G

I

V

A

A

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

T

S

P

H

L

L

T
|
T

L
x
V

T
x
G

D
x
Q

Q

Q

L
|
L

I
x
T

V

I

L

V

A
x
L

V

T

L

A

M

V

F

L

T

A

S
|
S

K
x
I

G
|
G

S
x
T

A

A

G
|
G

V
|
V

T

P

G
|
G

S
x
A

G
|
G

F

A

I

I

I

M

L

L

A

C

A

M

T

V

L

L

S

H

S
|
S

L

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

G

A

M

Y

V

A

L

M

L

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

I
x
M

T

V

N
|
N

T
x
V

I

T

G

G

N
x
D

G

L

V

T

G

G

T

T

M

A

A

I

I

V

A

A

K

K

W

T

V

E

G

G

A

T

L

L

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: K15 (≠ Q11), I18 (= I14), Y42 (≠ A32), P45 (= P35), F46 (≠ L36), F46 (≠ L36), F46 (≠ L36), L49 (≠ A39), L49 (≠ A39), L53 (= L43), I73 (≠ M63), P75 (≠ N65), K84 (≠ R75), L90 (≠ E81), L91 (≠ V82), L91 (≠ V82), F95 (≠ L86), L130 (≠ F128), V131 (≠ M129), I133 (≠ F131), L134 (≠ V132), I137 (= I135), L152 (= L150), F156 (≠ L154), I159 (= I157), I163 (≠ V161), I163 (≠ V161), I163 (≠ V161), A164 (= A162), Y167 (≠ H165), Y167 (≠ H165), K196 (≠ R186), I197 (= I187), N199 (= N189), G200 (≠ M190), Y204 (≠ L194), I207 (= I197), F210 (= F200), L250 (≠ I240), I253 (≠ Y243), Y254 (≠ S244), Y254 (≠ S244), I262 (≠ L252), K266 (= K256), M269 (≠ L259), T334 (= T324), V335 (≠ L325), G336 (≠ T326), Q337 (≠ D327), L339 (= L329), T340 (≠ I330), L343 (≠ A333), S369 (= S359), M399 (≠ I384)
binding aspartic acid: R276 (≠ S266), S277 (= S267), S278 (= S268), T314 (≠ Q304), T352 (≠ S342), G354 (= G344), V355 (= V345), G357 (= G347), A358 (≠ S348), G359 (= G349), D394 (≠ S379), R397 (= R382), T398 (≠ A383), N401 (= N386)
binding sodium ion: Y89 (≠ F80), T92 (≠ V83), S93 (= S84), G306 (= G296), A307 (≠ L297), T308 (= T298), N310 (= N300), N310 (= N300), M311 (≠ P301), D312 (= D302), S349 (= S339), I350 (≠ K340), G351 (= G341), T352 (≠ S342), N401 (= N386), V402 (≠ T387), D405 (≠ N390)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 3, 4, 4, 6, 7, 8

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
32% identity, 86% coverage: 11:399/451 of query aligns to 12:411/413 of 6x14A

query
sites
6x14A

Q

K

V
x
I

L

L

I

I

A

G

V

L

I

I

L

L

G

G

A

A

I

I

V

V

G

G

V

L

F

I

V

L

P

G

E

H

T

Y

G

G

-

Y

-

A

-

H

-

A

-

V

-

H

T

T

A

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F
|
F

I

V

K

R

L

L

I

L

K

C

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

V

V

T

V

G
|
G

I
x
A

A

A

R

S

M
x
I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L
x
V

I

V

Y

Y

F

Y

E

L

V

L

V

T

S

S

T

A

L

F

A

A

L

V

V

T

V

L

G

G

L

I

V

I

V

M

V

A

D

R

V

L

F

F

K

N

P

P

G

G

A

A

G

G

M

I

N

H

I

L

D

A

V

V

A

G

S

G

L

Q

D

Q

T

F

S

Q

S

P

L

H

A

-

T

-

Y

-

T

-

A

-

V

-

K

Q

H

A

A

P

S

P

F

L

M

V

D

H

F

I

V

L

M

L

N

D

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

G

A

A

F

L

A

A

K

N

G

G

N

Q

V

V

L

L

Q

P

I

T

L

I

L

F

F

F

S

A

I

I

L
x
I

L

L

G

G

V

I

A

A

L

I

A

T

H
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

P

K

R

S

A

A

K

E

V

T

F

L

V

L

D

D

T

A

L

I

D

N

S

G

L

L

M

A

Q

E

G

A

M

M

F

Y

R
x
K

I
|
I

V

V

N
|
N

M
x
G

V

V

M
|
M

R
x
Q

L
x
Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

H

S

V

L

V

F

G

S

E

L

L

G

A

K

K

L

V

M

T

A

A

C

A

V

V

Y

Y

L

V

T

G

C

L

A

T

V

L

F

Q

V

I

I

L

F

L

V

V

L

Y

G

F

P

V

I

L

C

L

R

K

Y

I

S

Y

G

G

F

I

S

D

L

P

W

I

K

S

F

F

L

I

K

K

F

H

I

A

K

K

E

D

E

A

L

M

F

L

T

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

Q

V

M

T

I

M

S

R

K

V

M

A

E

K

K

E

A

M

G

G

V

I

S

S

K

E

P

G

V

I

A

Y

G

S

M

F

I

T

I

L

P

P

S
x
L

G

G

L

A

T
|
T

F
x
I

N

N

P
x
M

D

D

G

G

Q
x
T

A

A

I

L

Y

Y

Y

Q

T

G

I

V

A

A

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

T

S

P

H

L

L

T

T

L

V

T

G

D

Q

Q

Q

L

L

I

T

V

I

L

V

A

L

V

T

L

A

M

V

F

L

T

A

S

S

K
x
I

G

G

S

T

A
|
A

G

G

V

V

T
x
P

G

G

S

A

G
|
G

F
x
A

I

I

I

M

L

L

A

C

A

M

T

V

L

L

S

H

S

S

L

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

G

A

M

Y

V

A

L

M

L

I

L
|
L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

I

M

T

V

N

N

T

V

I

T

G

G

N

D

G

L

V

T

G

G

T

T

M

A

A

I

I

V

A

A

K

K

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G59), A67 (≠ I60), I70 (≠ M63), V83 (≠ L77), I157 (≠ L158), Y164 (≠ H165), K193 (≠ R186), I194 (= I187), N196 (= N189), G197 (≠ M190), Q200 (≠ R193), Y201 (≠ L194), L302 (≠ S295), T305 (= T298), I306 (≠ F299), I347 (≠ K340), A350 (= A343)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ V12), F47 (= F40), M199 (= M192), R273 (≠ S266), S274 (= S267), S275 (= S268), M308 (≠ P301), T311 (≠ Q304), P353 (≠ T346), G356 (= G349), A357 (≠ F350), L384 (= L372), D391 (≠ S379), R394 (= R382), T395 (≠ A383)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 4

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
32% identity, 87% coverage: 11:402/451 of query aligns to 7:409/409 of 6bavA

query
sites
6bavA

Q

K

V

I

L

L

I

I

A

G

V

L

I

I

L

L

G

G

A

A

I

I

V

V

G

G

V

L

F

I

V

L

P

G

E

H

T

Y

G

G

-

Y

-

A

-

D

-

A

-

V

-

K

T

T

A

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M

I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L

V

I

V

Y

Y

F

Y

E

L

V

L

V

T

S

S

T

A

L

F

A

A

L

V

V

T

V

L

G

G

L

I

V

I

V

M

V

A

D

R

V

L

F

F

K

N

P

P

G

G

A

A

G

G

M

I

N

H

I

L

D

A

V

V

A

G

S

G

L

Q

D

Q

T

F

S

Q

S

P

L

-

A

-

T

-

Y

-

T

-

A

-

V

-

K

K

H

Q

A

A

S

P

-

P

F

L

M

V

D

K

F

I

V

L

M

L

N

D

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

G

A

A

F

L

A

A

K

N

G

G

N

Q

V

V

L

L

Q

P

I

T

L

I

L

F

F

F

S

A

I

I

L

I

L

L

G

G

V

I

A

A

L

I

A

T

H

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

P

K

R

S

A

A

K

E

V

T

F

L

V

L

D

D

T

A

L

I

D

N

S

G

L

L

M

A

Q

E

G

A

M

M

F

Y

R

K

I

I

V

V

N

N

M

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

K

S

V

L

V

F

G

S

E

L

L

G

A

K

K

L

V

M

T

A

A

C

A

V

V

Y

Y

L

V

T

G

C

L

A

T

V

L

F

Q

V

I

I

L

F

L

V

V

L

Y

G

F

P

V

I

L

C

L

R

K

Y

I

S

Y

G

G

F

I

S

D

L

P

W

I

K

S

F

F

L

I

K

K

F

K

I

A

K

K

E

D

E

A

L

M

F

L

T

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

Q

V

M

T

I

M

S

R

K

V

M

A

E

K

K

E

A

M

G

G

V

I

S

S

K

E

P

G

V

I

A

Y

G

S

M

F

I

T

I

L

P

P

S

L

G

G

L

A

T

T

F

I

N

N

P
x
M

D

D

G

G

Q
x
T

A

A

I

L

Y
|
Y

Y

Q

T

G

I

V

A

C

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

T

S

P

H

L

L

T

T

L

V

T

G

D

Q

Q

Q

L

L

I

T

V

I

L

V

A

L

V

T

L

A

M

V

F

L

T

A

S

S

K

I

G

G

S

T

A

A

G

G

V

V

T

P

G

G

S

A

G

G

F

A

I

I

I

M

L

L

A

A

A

M

T

V

L

L

S

E

S

S

L

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

G

A

M

Y

V

A

L

M

L

I

L

L

G

G

V

I

D
|
D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
x
C

A
x
T

I

M

T

V

N
|
N

T

V

I

T

G

G

N

D

G

L

V

T

G

G

T

T

M

A

A

I

I

V

A

A

K

K

W

T

V

E

G

G

binding benzylcysteine: R268 (≠ S266), S269 (= S267), S270 (= S268), M303 (≠ P301), T306 (≠ Q304), Y309 (= Y307), D382 (= D375), D386 (≠ S379), C389 (≠ R382), T390 (≠ A383), N393 (= N386)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
32% identity, 86% coverage: 11:399/451 of query aligns to 7:406/408 of 6bauA

query
sites
6bauA

Q

K

V

I

L

L

I

I

A

G

V

L

I

I

L

L

G

G

A

A

I

I

V

V

G

G

V

L

F

I

V

L

P

G

E

H

T

Y

G

G

-

Y

-

A

-

D

-

A

-

V

-

K

T

T

A

Y

L

V

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

F

L

A

T

S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M

I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L

V

I

V

Y

Y

F

Y

E

L

V

L

V

T

S

S

T

A

L

F

A

A

L

V

V

T

V

L

G

G

L

I

V

I

V

M

V

A

D

R

V

L

F

F

K

N

P

P

G

G

A

A

G

G

M

I

N

H

I

L

D

A

V

V

A

G

S

G

L

Q

D

Q

T

F

S

Q

S

P

L

-

A

-

T

-

Y

-

T

-

A

-

V

-

K

K

H

Q

A

A

S

P

-

P

F

L

M

V

D

K

F

I

V

L

M

L

N

D

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

G

A

A

F

L

A

A

K

N

G

G

N

Q

V

V

L

L

Q

P

I

T

L

I

L

F

F

F

S

A

I

I

L

I

L

L

G

G

V

I

A

A

L

I

A

T

H

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

V

V

G

R

P

K

R

S

A

A

K

E

V

T

F

L

V

L

D

D

T

A

L

I

D

N

S

G

L

L

M

A

Q

E

G

A

M

M

F

Y

R

K

I

I

V

V

N

N

M

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

K

S

V

L

V

F

G

S

E

L

L

G

A

K

K

L

V

M

T

A

A

C

A

V

V

Y

Y

L

V

T

G

C

L

A

T

V

L

F

Q

V

I

I

L

F

L

V

V

L

Y

G

F

P

V

I

L

C

L

R

K

Y

I

S

Y

G

G

F

I

S

D

L

P

W

I

K

S

F

F

L

I

K

K

F

K

I

A

K

K

E

D

E

A

L

M

F

L

T

T

V

A

L

F

G

V

T

T

S
x
R

S

S

S
|
S

E

S

S

G

V

T

L

L

P

P

Q

V

M

T

I

M

S

R

K

V

M

A

E

K

K

E

A

M

G

G

V

I

S

S

K

E

P

G

V

I

A

Y

G

S

M

F

I

T

I

L

P

P

S

L

G

G

L

A

T

T

F

I

N

N

P
x
M

D

D

G

G

Q
x
T

A

A

I

L

Y

Y

Y

Q

T

G

I

V

A

C

A

T

I

F

F

F

I

I

A

A

Q

N

A

A

T

L

N

G

T

S

P

H

L

L

T

T

L

V

T

G

D

Q

Q

Q

L

L

I

T

V

I

L

V

A

L

V

T

L

A

M

V

F

L

T

A

S

S

K

I

G

G

S

T

A
|
A

G
|
G

V
|
V

T
x
P

G

G

S

A

G
|
G

F

A

I

I

I

M

L

L

A

A

A

M

T

V

L

L

S

E

S

S

L

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

G

A

M

Y

V

A

L

M

L

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
x
C

A
x
T

I

M

T

V

N
|
N

T

V

I

T

G

G

N

D

G

L

V

T

G

G

T

T

M

A

A

I

I

V

A

A

K

K

binding cysteine: R268 (≠ S266), S270 (= S268), M303 (≠ P301), T306 (≠ Q304), A345 (= A343), G346 (= G344), V347 (= V345), P348 (≠ T346), G351 (= G349), D386 (≠ S379), C389 (≠ R382), T390 (≠ A383), N393 (= N386)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
32% identity, 88% coverage: 11:408/451 of query aligns to 6:412/416 of 6r7rA

query
sites
6r7rA

Q

K

V

I

L

L

I

W

A

G

V

L

I

V

L

L

G

G

A

A

I

V

V

F

G

G

V

L

F

I

V

A

P

G

E
x
H

T

F

G

G

-

Y

-

A

-

G

-

A

-

V

-

K

T

T

A

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M

I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L

V

I

V

Y

Y

F

Y

E

L

V

A

V

T

S

S

T

A

L

M

A

A

L

V

V

F

G

G

L

L

V

I

V

V

V

G

D

R

V

L

F

F

K

N

P

V

G

G

A

A

G

N

M

V

N

N

I

L

D

G

V

S

A

G

S

-

L

-

D

-

T

-

S

-

L

-

A

-

T

-

Y

-

T

-

T

T

A

G

V

A

K

Q

H

P

A

P

S

S

F

L

M

V

D

Q

F

T

V

L

M

L

N

N

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

A

A

S

F

L

A

A

K

K

G

G

N

E

V

V

L

L

Q

P

I

V

L

I

L

F

F

F

S

A

I

I

L

I

L

L

G

G

V

I

A

A

L

I

A

T

H

Y

V

L

G

M

P

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

K

E

V

T

F

L

V

L

D

R

T

V

L

F

D

D

S

G

L

L

M

A

Q

E

G

A

M

M

F

Y

R

L

I

I

V

V

N

G

M

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

R

S
x
V

L
x
V

F

G

S

P

L

L

G
x
A

K
|
K

L

V

M

V

A
x
G

C

A

V

V

Y

Y

L

T

T

G

C

L

A

F

V

L

F

Q

V

I

I

V

F

I

V

T

L

Y

G

F

P

I

I

L

C

L

R

K

Y

V

S

F

G

G

F

I

S

D

L

P

W

I

K

K

F

F

L

I

K

R

F

K

I

A

K

K

E

D

E

A

L

M

F

I

T

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

-

V

Q

T

M

M

I

R

S

V

K

A

M

E

E

E

K

E

A

M

G

G

V

V

S

D

K

K

P

G

V

I

A

F

G

S

M

F

I

T

I

L

P

P

S

L

G

G

L

A

T

T

F

I

N

N

P
x
M

D

D

G

G

Q
x
T

A

A

I

L

Y

Y

Y

Q

T

G

I

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

A

A

T

I

N

G

T

H

P

P

L

L

T

T

L

L

T

G

D

Q

Q

Q

L

L

I

V

V

V

L

V

A

L

V

T

L

A

M

V

F

L

T

A

S

S

K

I

G

G

S
x
T

A
|
A

G
|
G

V
|
V

T

P

G

G

S

A

G
|
G

F

A

I

I

I

M

L

L

A

A

A

M

T

V

L

L

S

Q

S

S

L

V

G

G

T

L

-

D

-

L

-

T

-

P

-

G

I

S

P

P

V

V

A

A

-

L

G

A

M

Y

V

A

L

M

L

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

I

M

T

V

N
|
N

T

V

I

T

G

G

N

D

G

L

V

A

G

G

T

T

M

V

A

I

I

V

A

A

K

K

W

T

V

E

G

K

A

E

L

L

D

D

T

E

V

S

K

K

binding d-aspartic acid: R263 (≠ S266), S264 (= S267), S265 (= S268), M299 (≠ P301), T302 (≠ Q304), T340 (≠ S342), A341 (= A343), G342 (= G344), V343 (= V345), G347 (= G349), D383 (≠ S379), R386 (= R382), T387 (≠ A383), N390 (= N386)
binding decyl-beta-d-maltopyranoside: H23 (≠ E28), V211 (≠ S214), V212 (≠ L215), A216 (≠ G219), K217 (= K220), G220 (≠ A223)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
32% identity, 88% coverage: 11:408/451 of query aligns to 10:420/424 of 6zl4A

query
sites
6zl4A

Q

K

V

I

L

L

I

W

A

G

V

L

I

V

L

L

G

G

A

A

I

V

V

F

G

G

V

L

F

I

V

A

P

G

E

H

T

F

G

G

-

Y

-

A

-

G

-

A

-

V

-

K

T

T

A

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M

I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L

V

I

V

Y

Y

F

Y

E

L

V

A

V

T

S

S

T

A

L

M

A

A

L

V

V

F

G

G

L

L

V

I

V

V

V

G

D

R

V

L

F

F

K

N

P

V

G

G

A

A

G

N

M

V

N

N

I

L

D

-

V

-

A

-

S

-

L

-

D

-

T

-

S

G

S

S

L

G

A

T

T

G

Y

K

T

A

T

I

A

E

V

A

K

Q

H

P

A

P

S

S

F

L

M

V

D

Q

F

T

V

L

M

L

N

N

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

A

A

S

F

L

A

A

K

K

G

G

N

E

V

V

L

L

Q

P

I

V

L

I

L

F

F

F

S

A

I

I

L

I

L

L

G

G

V

I

A

A

L

I

A

T

H

Y

V

L

G

M

P

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

K

E

V

T

F

L

V

L

D

R

T

V

L

F

D

D

S

G

L

L

M

A

Q

E

G

A

M

M

F
x
Y

R
x
L

I

I

V

V

N
x
G

M
x
G

V

V

M

M

R
x
Q

L

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

R

S

V

L

V

F

G

S

P

L

L

G

A

K

K

L

V

M

V

A

G

C

A

V

V

Y

Y

L

T

T

G

C

L

A

F

V

L

F

Q

V

I

I

V

F

I

V

T

L

Y

G

F

P

I

I

L

C

L

R

K

Y

V

S

F

G

G

F

I

S

D

L

P

W

I

K

K

F

F

L

I

K

R

F

K

I

A

K

K

E

D

E

A

L

M

F

I

T

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

-

V

Q

T

M

M

I
x
R

S

V

K

A

M

E

E
|
E

K

E

A

M

G

G

V

V

S

D

K

K

P

G

V

I

A

F

G

S

M

F

I

T

I

L

P

P

S

L

G

G

L

A

T

T

F

I

N

N

P
x
M

D

D

G

G

Q
x
T

A

A

I

L

Y

Y

Y

Q

T

G

I

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

A

A

T

I

N

G

T

H

P

P

L

L

T

T

L

L

T

G

D

Q

Q

Q

L

L

I

V

V

V

L

V

A

L

V

T

L

A

M

V

F

L

T

A

S

S

K

I

G

G

S

T

A

A

G

G

V
|
V

T
x
P

G
|
G

S
x
A

G
|
G

F

A

I

I

I

M

L

L

A

A

A

M

T

V

L

L

S

Q

S

S

L

V

G

G

T

L

-

D

-

L

-

T

-

P

-

G

I

S

P

P

V

V

A

A

-

L

G

A

M

Y

V

A

L

M

L

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

I

M

T

V

N
|
N

T

V

I

T

G

G

N

D

G

L

V

A

G

G

T

T

M

V

A

I

I

V

A

A

K

K

W

T

V

E

G

K

A

E

L

L

D

D

T

E

V

S

K

K

binding decyl-beta-d-maltopyranoside: Y190 (≠ F185), L191 (≠ R186), G194 (≠ N189), G195 (≠ M190), Q198 (≠ R193), R282 (≠ I276), E286 (= E280)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S266), S272 (= S267), S273 (= S268), M307 (≠ P301), T310 (≠ Q304), V351 (= V345), P352 (≠ T346), G353 (= G347), A354 (≠ S348), G355 (= G349), D391 (≠ S379), R394 (= R382), T395 (≠ A383), N398 (= N386)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
32% identity, 88% coverage: 11:408/451 of query aligns to 11:421/425 of 6zgbA

query
sites
6zgbA

Q

K

V

I

L

L

I

W

A

G

V

L

I

V

L

L

G

G

A

A

I

V

V

F

G

G

V

L

F

I

V

A

P

G

E

H

T

F

G

G

-

Y

-

A

-

G

-

A

-

V

-

K

T

T

A

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M

I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L

V

I

V

Y

Y

F

Y

E

L

V

A

V

T

S

S

T

A

L

M

A

A

L

V

V

F

G

G

L

L

V

I

V

V

V

G

D

R

V

L

F

F

K

N

P

V

G

G

A

A

G

N

M

V

N

N

I

L

D

-

V

-

A

-

S

-

L

-

D

-

T

-

S

G

S

S

L

G

A

T

T

G

Y

K

T

A

T

I

A

E

V

A

K

Q

H

P

A

P

S

S

F

L

M

V

D

Q

F

T

V

L

M

L

N

N

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

A

A

S

F

L

A

A

K

K

G

G

N

E

V

V

L

L

Q

P

I

V

L

I

L

F

F

F

S

A

I

I

L

I

L

L

G

G

V

I

A

A

L

I

A

T

H

Y

V

L

G

M

P

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

K

E

V

T

F

L

V

L

D

R

T

V

L

F

D

D

S

G

L

L

M

A

Q

E

G

A

M

M

F

Y

R

L

I

I

V

V

N

G

M

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

R

S

V

L

V

F

G

S

P

L

L

G

A

K

K

L

V

M

V

A

G

C

A

V

V

Y

Y

L

T

T

G

C

L

A

F

V

L

F

Q

V

I

I

V

F

I

V

T

L

Y

G

F

P

I

I

L

C

L

R

K

Y

V

S

F

G

G

F

I

S

D

L

P

W

I

K

K

F

F

L

I

K

R

F

K

I

A

K

K

E

D

E

A

L

M

F

I

T

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

-

V

Q

T

M

M

I

R

S

V

K

A

M

E

E

E

K

E

A

M

G

G

V

V

S

D

K

K

P

G

V

I

A

F

G

S

M

F

I

T

I

L

P

P

S

L

G

G

L

A

T

T

F

I

N

N

P
x
M

D

D

G

G

Q
x
T

A

A

I

L

Y

Y

Y

Q

T

G

I

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

A

A

T

I

N

G

T

H

P

P

L

L

T

T

L

L

T

G

D

Q

Q

Q

L

L

I

V

V

V

L

V

A

L

V

T

L

A

M

V

F

L

T

A

S

S

K

I

G

G

S

T

A

A

G

G

V

V

T

P

G

G

S

A

G

G

F

A

I

I

I

M

L

L

A

A

A

M

T

V

L

L

S

Q

S

S

L

V

G

G

T

L

-

D

-

L

-

T

-

P

-

G

I

S

P

P

V

V

A

A

-

L

G

A

M

Y

V

A

L

M

L

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

I

M

T

V

N

N

T

V

I

T

G

G

N

D

G

L

V

A

G

G

T

T

M

V

A

I

I

V

A

A

K

K

W

T

V

E

G

K

A

E

L

L

D

D

T

E

V

S

K

K

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ S266), S273 (= S267), S274 (= S268), M308 (≠ P301), T311 (≠ Q304), D392 (≠ S379), R395 (= R382), T396 (≠ A383)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
32% identity, 88% coverage: 11:408/451 of query aligns to 13:423/426 of 6xwnB

query
sites
6xwnB

Q

K

V

I

L

L

I

W

A

G

V

L

I

V

L

L

G

G

A

A

I

V

V

F

G

G

V

L

F

I

V

A

P

G

E

H

T

F

G

G

-

Y

-

A

-

G

-

A

-

V

-

K

T

T

A

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M

I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L

V

I

V

Y

Y

F

Y

E

L

V

A

V

T

S

S

T

A

L

M

A

A

L

V

V

F

G

G

L

L

V

I

V

V

V

G

D

R

V

L

F

F

K

N

P

V

G

G

A

A

G

N

M

V

N

N

I

L

D

-

V

-

A

-

S

-

L

-

D

-

T

-

S

G

S

S

L

G

A

T

T

G

Y

K

T

A

T

I

A

E

V

A

K

Q

H

P

A

P

S

S

F

L

M

V

D

Q

F

T

V

L

M

L

N

N

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

A

A

S

F

L

A

A

K

K

G

G

N

E

V

V

L

L

Q

P

I

V

L

I

L

F

F

F

S

A

I

I

L

I

L

L

G

G

V

I

A

A

L

I

A

T

H

Y

V

L

G

M

P

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

K

E

V

T

F

L

V

L

D

R

T

V

L

F

D

D

S

G

L

L

M

A

Q

E

G

A

M

M

F

Y

R

L

I

I

V

V

N

G

M

G

V

V

M

M

R

Q

L

Y

A

A

P

P

I

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

I

M

G

A

K

E

Y

Q

G

G

F

V

G

R

S

V

L

V

F

G

S

P

L

L

G

A

K

K

L

V

M

V

A

G

C

A

V

V

Y

Y

L

T

T

G

C

L

A

F

V

L

F

Q

V

I

I

V

F

I

V

T

L

Y

G

F

P

I

I

L

C

L

R

K

Y

V

S

F

G

G

F

I

S

D

L

P

W

I

K

K

F

F

L

I

K

R

F

K

I

A

K

K

E

D

E

A

L

M

F

I

T

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

V

T

L

L

P

P

-

V

Q

T

M

M

I

R

S

V

K

A

M

E

E

E

K

E

A

M

G

G

V

V

S

D

K

K

P

G

V

I

A

F

G

S

M

F

I

T

I

L

P

P

S

L

G

G

L

A

T
|
T

F

I

N

N

P
x
M

D

D

G

G

Q
x
T

A

A

I

L

Y

Y

Y

Q

T

G

I

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

A

A

T

I

N

G

T

H

P

P

L

L

T

T

L

L

T

G

D

Q

Q

Q

L

L

I

V

V

V

L

V

A

L

V

T

L

A

M

V

F

L

T

A

S

S

K

I

G

G

S

T

A

A

G

G

V

V

T

P

G

G

S

A

G
|
G

F

A

I

I

I

M

L

L

A

A

A

M

T

V

L

L

S

Q

S

S

L

V

G

G

T

L

-

D

-

L

-

T

-

P

-

G

I

S

P

P

V

V

A

A

-

L

G

A

M

Y

V

A

L

M

L

I

L

L

G

G

V

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

I

M

T

V

N

N

T

V

I

T

G

G

N

D

G

L

V

A

G

G

T

T

M

V

A

I

I

V

A

A

K

K

W

T

V

E

G

K

A

E

L

L

D

D

T

E

V

S

K

K

binding (3s)-3-(benzyloxy)-l-aspartic acid: R274 (≠ S266), S275 (= S267), S276 (= S268), T307 (= T298), M310 (≠ P301), T313 (≠ Q304), G358 (= G349), D394 (≠ S379), R397 (= R382), T398 (≠ A383)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
32% identity, 88% coverage: 11:408/451 of query aligns to 13:423/427 of 5e9sA

query
sites
5e9sA

Q

K

V

I

L

L

I

W

A

G

V

L

I

V

L

L

G

G

A

A

I

V

V

F

G

G

V

L

F

I

V

A

P

G

E

H

T

F

G

G

-

Y

-

A

-

G

-

A

-

V

-

K

T

T

A

Y

L

I

K

K

P

P

L

F

G

G

D

D

A

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

L

L

L

V

A

M

P

P

V

I

I

V

F

L

L

A

T

S

V

L

V

V

T

V

G

G

I

A

A

A

R

S

M

I

E

S

N

P

M

A

K

R

E

-

L

L

G

G

R

R

V

V

G

G

F

V

R

K

A

I

L

V

I

V

Y

Y

F
x
Y

E

L

V

A

V
x
T

S
|
S

T

A

L

M

A

A

L

V

V

F

G

G

L

L

V

I

V

V

V

G

D

R

V

L

F

F

K

N

P

V

G

G

A

A

G

N

M

V

N

N

I

L

D

-

V

-

A

-

S

-

L

-

D

-

T

-

S

G

S

S

L

G

A

T

T

G

Y

K

T

A

T

I

A

E

V

A

K

Q

H

P

A

P

S

S

F

L

M

V

D

Q

F

T

V

L

M

L

N

N

I

I

I

V

P

P

D

T

T

N

I

P

V

F

D

A

A

S

F

L

A

A

K

K

G

G

N

E

V

V

L

L

Q

P

I

V

L

I

L

F

F

F

S

A

I

I

L

I

L

L

G

G

V

I

A

A

L

I

A

T

H

Y

V

L

G

M

P

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

K

E

V

T

F

L

V

L

D

R

T

V

L

F

D

D