SitesBLAST

HLN 66:68 (= HLN 69:71) binding
Y138 (= Y141) binding
GFA 199:201 (= GFA 202:204) binding
N203 (= N206) binding
R206 (= R209) binding
PL 236:237 (≠ PV 239:240) mutation to FW: No detectable water or glycerol permeability.

1fx8A Crystal structure of the e. Coli glycerol facilitator (glpf) with substrate glycerol (see paper)
72% identity, 90% coverage: 9:262/283 of query aligns to 1:254/254 of 1fx8A

query
sites
1fx8A

S

T

L

L

P

K

S

G

Q

Q

C

C

L

I

A

A

E

E

F

F

L

L

G

G

T

T

A

G

L

L

I

I

F

F

G

G

T

V

G

G

C

C

V

V

A

A

L

L

K

K

V

V

A

A

G

G

A

A

S

S

F

F

G

G

L

Q

W

W

E

E

I

I

S

S

L

V

I

I

W
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W

G

G

V

L

G

G

V
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V

S

A

M

M

A

A

I

I

Y

Y

L

L

S

T

A

A

G

G

I

V

S

S

G

G

A

A

H
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H

L
|
L

N
|
N

P

P

A

A

V

V

S

T

I

I

A

A

L

L

C

W

I

L

F

F

T

A

G

C

F

F

E

D

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K

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R

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K

L

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I

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Y

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M

I

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Q

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V

A

A

G

G

A

A

F

F

C

C

G

A

A

A

F

A

L

L

V

V

Y

Y

T

G

L

L

Y

Y

S

Y

N

N

L

L

F

F

D

D

F

F

E

E

Q

Q

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R

H

H

H

M

I

I

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R

R

G

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A

E

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E

D

L

L

A

A

S

G

V

T

F

F

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S

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T

Y
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Y

P
|
P

H
x
N

A
x
P

A

H

L

I

S

N

T

F

G

V

Q

Q

A

A

F

F

L

A

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V

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E

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M

I

V

I

I

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T

A

A

I

I

L

L

M

M

G

G

V

L

I

I

M

L

S

A

L

L

T

T

D

D

N

G

N

N

G

G

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P

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R

G

G

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P

L

L

A

A

P

P

L

L

I

I

G

G

L

L

I
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I

A

A

V

V

I

I

G

G

S

A

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S

M

M

G
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G

P
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P

L

L

T

T

G
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G

F
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F

A
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A

M

M

N
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N

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A

A

R
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R

D

D

F

F

G

G

P

P

K

K

L

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M

F

T

A

F

W

F

L

T

A

G

G

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W

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G

E

N

I

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S

A

L

F

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T

G

G

R

R

D

D

I

I

P

P

Y

Y

F

F

L

L

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V

P

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V

L

F

F

A

G

P

P

I

I

V

V

G

G

A

A

C

I

L

V

G

G

A

A

A

F

A

A

Y

Y

R

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L

L

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I

A

G

R

R

H

H

L

L

binding glycerol: W43 (= W51), V47 (= V55), H61 (= H69), L62 (= L70), N63 (= N71), Y133 (= Y141), P134 (= P142), N135 (≠ H143), P136 (≠ A144), I182 (= I190), G190 (= G198), P191 (= P199), G194 (= G202), G194 (= G202), F195 (= F203), A196 (= A204), N198 (= N206), R201 (= R209)

B1VB61 Propanediol uptake facilitator PduF from Citrobacter freundii (see paper)
64% identity, 95% coverage: 6:273/283 of query aligns to 1:268/269 of B1VB61

query
sites
B1VB61

Q

M

Q

N

P

D

S

S

L

L

P

K

S

A

Q

Q

C

C

L

I

A

A

E

E

F

F

L

L

G

G

T

T

A

G

L

L

L

F

I

L

F

F

G

G

T

I

G

G

C

C

V

L

A

S

A

A

L

L

K

K

V

V

A

A

G

G

A

A

S

S

F

L

G

G

L

L

W

W

E

E

I

I

S

C

L

I

I

I

W

W

G

G

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L

G

G

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I

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S

M

L

A

A

I

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Y

Y

L

L

S

T

A

A

G

G

I

I

S

S

G

G

A

A

H

H

L

L

N
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N

P
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P

A
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A

V

I

S

T

I

I

A

A

L

L

C

W

I

L

F

F

T

A

G

C

F

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E

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G

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R

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K

L

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F

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Y

Y

M

T

L

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S

A

Q

Q

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V

A

A

G

G

A

A

F

F

C

G

G

G

A

A

F

L

L

L

V

A

Y

Y

T

L

L

L

Y

Y

S

G

N

S

L

L

F

F

F

T

D

E

F

Y

E

E

Q

S

S

A

R

H

H

Q

M

M

I

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R

G

G

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S

Q

L

A

E

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E

H

L

L

A

A

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I

F

F

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T

Y

Y

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P

H

A

A

A

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L

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V

G

W

Q

Q

A

A

F

A

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L

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V

E

E

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V

I

V

I

I

T

T

A

S

I

I

L

L

M

M

G

G

V

M

I

I

M

M

S

A

L

L

T

T

D

D

N

G

N

N

G

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R

K

G

G

P

P

L

L

A

A

P

P

L

L

I

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G

G

L

I

L

L

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A

A

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V

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I

G

G

S

A

S

S

M

T

G

G

P

P

L

L

T

T

G

G

F

F

A

A

M

M

N
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N

P
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P

A
|
A

R

R

D

D

F

F

G

G

P

P

K

K

L

L

M

F

T

A

F

W

F

M

T

A

G

G

W

W

G

G

E

D

I

V

S

A

L

M

T

T

G

G

R

R

D

D

I

I

P

P

Y

Y

F

F

L

I

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V

P

P

V

I

F

V

A

A

P

P

I

I

V

I

G

G

A

A

C

C

L

A

G

G

A

A

A

I

Y

Y

R

R

G

Y

L

L

I

I

A

G

R

K

H

N

L

L

P

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S

C

A

N

I

T

T

C

A

K

T

L

K

D

D

E

A

N

E

E

NPA 66:68 (= NPA 71:73) NPA 1
NPA 201:203 (= NPA 206:208) NPA 2

P37451 Propanediol uptake facilitator PduF from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
66% identity, 91% coverage: 6:263/283 of query aligns to 1:258/264 of P37451

query
sites
P37451

Q

M

Q

N

P

D

S

S

L

L

P

K

S

A

Q

Q

C

C

L

G

A

A

E

E

F

F

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L

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T

T

A

G

L

L

L

F

I

L

F

F

G

G

T

I

G

G

C

C

V

L

A

S

A

A

L

L

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K

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V

A

A

G

G

A

A

S

S

F

L

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G

L

L

W

W

E

E

I

I

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C

L

I

I

I

W

W

G

G

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L

G

G

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M

L

A

A

I

V

Y

Y

L

L

S

T

A

A

G

G

I

I

S

S

G

G

A

G

H

H

L

L

N
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N

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P

A
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A

V

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T

I

I

A

A

L

L

C

W

I

L

F

F

T

A

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C

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Q

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A

Q

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F

A

A

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A

A

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C

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G

A

A

F

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Y

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N

S

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L

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E

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F

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E

Q

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H

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L

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F

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Y

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H

A

A

A

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Q

Q

A

A

F

A

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L

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E

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I

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L

M

M

G

G

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M

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M

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A

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L

T

T

D

D

N

G

N

N

G

G

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I

P

P

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K

G

G

P

P

L

L

A

A

P

P

L

L

I

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G

G

L

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L

L

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V

A

A

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I

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G

S

A

S

S

M

T

G

G

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P

L

L

T

T

G

G

F

F

A

A

M

M

N
|
N

P
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P

A
|
A

R

R

D

D

F

F

G

G

P

P

K

K

L

L

M

F

T

T

F

W

F

L

T

A

G

G

W

W

G

G

E

N

I

M

S

A

L

M

T

S

G

G

R

R

D

E

I

I

P

P

Y

Y

F

F

L

I

V

V

P

P

V

I

F

V

A

A

P

P

I

V

V

I

G

G

A

A

C

C

L

A

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G

A

A

A

I

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Y

R

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Y

L

F

I

I

A

G

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H

N

L

L

P

P

NPA 66:68 (= NPA 71:73) NPA 1
NPA 201:203 (= NPA 206:208) NPA 2

Q96PS8 Aquaporin-10; AQP-10; Aquaglyceroporin-10; Small intestine aquaporin from Homo sapiens (Human) (see 2 papers)
39% identity, 93% coverage: 10:271/283 of query aligns to 20:277/301 of Q96PS8

query
sites
Q96PS8

L

L

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A

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Q

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C

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L

A

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E

F

F

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L

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L

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F

L

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T

Q

G

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C

A

V

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A

A

A

Q

L

A

K

V

V

T

A

S

G

G

A

E

S

T

F

K

G

G

-

N

L

F

W

F

E

T

I

M

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F

L

L

I

A

W

G

G

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L

G

A

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T

M

I

A

A

I

I

Y

Y

L

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S
x
G

A

G

G

N

I

V

S
|
S

G

G

A

A

H
|
H

L

L

N

N

P

P

A

A

V
x
F

S

S

I

L

A

A

L

M

C

C

I

I

F

V

T

G

G
x
R

F

L

E

P

K

W

R

V

K

K

L

L

P

P

F

I

Y

Y

M

I

L

L

S

V

Q

Q

V

L

A

L

G

S

A

A

F

F

C

C

G

A

A

S

F

G

L

A

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Y

Y

T

V

L

L

Y

Y

S

H

N

D

L

A

F

L

F

Q

D

N

F

Y

E

T

Q

G

S

G

R
x
N

H

L

M

T

I

V

R

T

G

G

T

P

Q

K

A

-

S

-

L

-

E

E

L

T

A

A

S

S

V

I

F

F

S

A

T

T

Y

Y

P

P

H

A

A

P

A

Y

L

L

S

S

T

L

G

N

Q

N

A

G

F

F

L

L

V

D

E

Q

V

V

I

L

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G

T

T

A

G

I

M

L

L

M

I

-

V

G

G

V

L

I

L

M

A

S

I

L

L

T

D

D

R

N

N

N

K

G

G

L

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P

R

A

G

G

P

-

L

L

A

E

P

P

L

V

I

V

G

G

L

M

L

L

I

I

A

L

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A

I

L

G

G

S

L

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S

M

M

G

G

P

A

L

N

T

C

G

G

F

I

A

P

M

L

N

N

P

P

A

A

R

R

D

D

F

L

G

G

P

P

K

R

L

L

M

F

T

T

F

Y

F

V

T

A

G

G

W

W

G

G

E

P

I

E

S

V

L

F

T

S

G

A

G

G

R

N

D

G

I

-

P

-

Y

W

F

W

L

W

V

V

P

P

V

V

F

V

A

A

P

P

I

L

V

V

G

G

A

A

C

T

L

V

G

G

A

T

A

A

A

T

Y

Y

R

Q

G

L

L

L

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V

A

A

R

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H

L

H

P

P

S

E

A

G

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E

A

P

T

A

K

Q

D

D

E27 (= E17) mutation to Q: Abolishes permeability to glycerol.
G73 (≠ S62) mutation to A: Increased permeability to glycerol at acidic pH.; mutation to F: Abolishes permeability to glycerol.
S77 (= S66) mutation S->A,D: Nearly abolishes permeability to glycerol.
H80 (= H69) mutation to A: Abolishes permeability to glycerol.
F85 (≠ V74) mutation to A: Nearly abolishes permeability to glycerol.
R94 (≠ G83) mutation to A: Abolishes permeability to glycerol.
N133 (≠ R122) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Abolishes N-glycosylation.

6f7hD Crystal structure of human aqp10 (see paper)
40% identity, 89% coverage: 10:261/283 of query aligns to 8:255/256 of 6f7hD

query
sites
6f7hD

L

L

P

A

S

R

Q

Q

C

C

L

L

A

A

E

E

F

F

L

L

G

G

T

V

A

F

L

V

L

L

I

M

F

L

G

T

T

Q

G

G

C

A

V

V

A

A

A

Q

L

A

K

V

V

T

A

S

G

G

A

E

S

T

F

K

G

G

-

N

L

F

W

F

E

T

I

M

S

F

L

L

I

A

W

G

G

S

V

L

G

A

V

V

S

T

M

I

A

A

I

I

Y

Y

L

V

S

G

A

G

G

N

I

V

S

S

G

G

A

A

H
|
H

L

L

N
|
N

P

P

A

A

V

F

S

S

I

L

A

A

L

M

C

C

I

I

F

V

T

G

G

R

F

L

E

P

K

W

R

V

K

K

L

L

P

P

F

I

Y

Y

M

I

L

L

S

V

Q

Q

V

L

A

L

G

S

A

A

F

F

C

C

G

A

A

S

F

G

L

A

V

T

Y

Y

T

V

L

L

Y

Y

S

H

N

D

L

A

F

L

F

Q

D

N

F

Y

E

T

Q

G

S

G

R

N

H

L

M

T

I

V

R

T

G

G

T

P

Q

K

A

-

S

-

L

-

E

E

L

T

A

A

S

S

V

I

F

F

S

A

T

T

Y

Y

P

P

H

A

A

P

A

Y

L

L

S

S

T

L

G

N

Q

N

A

G

F

F

L

L

V

D

E

Q

V

V

I

L

I

G

T

T

A

G

I

M

L
|
L

M

I

-

V

G

G

V
x
L

I

L

M

A

S

I

L

L

T

D

D

R

N

N

N

K

G

G

L

V

P

P

R

A

G

G

P

-

L

L

A

E

P

P

L

V

I

V

G

G

L

M

L

L

I

I

A

L

V

A

I

L

G

G

S

L

S

S

M

M

G

G

P

A

L

N

T

C

G

G

F

I

A

P

M

L

N
|
N

P

P

A

A

R

R

D

D

F

L

G

G

P

P

K

R

L

L

M

F

T

T

F

Y

F

V

T

A

G

G

W

W

G

G

E

P

I

E

S

V

L

F

T

S

G

A

G

G

R

N

D

G

I

-

P

-

Y

W

F

W

L

W

V

V

P

P

V

V

F

V

A

A

P

P

I

L

V

V

G

G

A

A

C

T

L

V

G

G

A

T

A

A

A

T

Y

Y

R

Q

G

L

L

L

I

V

A

A

R

L

H

H

binding glycerol: H68 (= H69), N70 (= N71), L158 (= L162), L162 (≠ V165), N202 (= N206)

O14520 Aquaporin-7; AQP-7; Aquaglyceroporin-7; Aquaporin adipose; AQPap; Aquaporin-7-like from Homo sapiens (Human) (see 4 papers)
34% identity, 94% coverage: 7:272/283 of query aligns to 30:290/342 of O14520

query
sites
O14520

Q
|
Q

P
x
R

S
x
K

L

M

P

V

S

R

Q

E

C

F

L

L

A

A

E

E

F

F

L

M

G

S

T

T

A

Y

L

V

L

M

I

M

F

V

F

F

G

G

T

L

G

G

C

S

V

V

A

A

A

H

L

M

-
x
V

-

L

-

N

K

K

V

K

A

Y

G

G

A

S

S

Y

F

L

G

G

L

-

W

-

E

-

I

V

S

N

L

L

I

G

W

F

G

G

V

F

G

G

V

V

S

T

M

M

A

G

I

V

Y

H

L

V

S

A

A

G

G

R

I

I

S

S

G

G

A

A

H

H

L

M

N

N

P

A

A

A

V

V

S

T

I

F

A

A

L

N

C

C

I

A

F

L

T

G

G

R

F

V

E

P

K

W

R

R

K

K

L

F

P

P

F

V

Y

Y

M

V

L

L

S

G

Q

Q

V

F

A

L

G

G

A

S

F

F

C

L

G

A

A

A

F

A

L

T

V

I

Y

Y

T

S

L

L

Y

F

S
x
Y

N

T

L

A

F

I

F

L

D

H

F

F

E

S

Q

G

S

G

R

Q

H

L

M

M

I

V

R

T

G

G

T

P

Q

V

A

A

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

A

T

T

Y

Y

P

L

H

P

A

D

A
x
H

L

M

S

T

T

L

G

W

Q

R

A

G

F

F

L

L

V

N

E

E

V

A

I

W

I

L

T

T

A

G

I

M

L

L

M

Q

G

L

V

C

I

L

M

F

S

A

L

I

T

T

D

D

D

Q

N

E

N

N

G

N

L

P

P

A

R

L

G

P

P

G

L

T

A

E

P

A

L

L

L

V

I

I

G

G

L

I

L

L

I

V

A

V

V

I

I

I

G

G

S

V

S

S

M

L

G

G

P

M

L

N

T

T

G

G

F

Y

A

A

M

I

N

N

P

P

A

S

R

R

D

D

F

L

G

P

P

P

K

R

L

I

M

F

T

T

F

F

F

I

T

A

G

G

W

W

G

G

E

K

I

Q

S

V

L

F

T

S

G

N

G

G

R

E

D

N

I

-

P

-

Y

W

F

W

L

W

V

V

P

P

V

V

F

V

A

A

P

P

I

L

V

L

G
|
G

A

A

C

Y

L

L

G

G

A

G

A

I

Y

Y

R

L

G

V

L

F

I

I

A

G

R

S

H

T

L

I

P

P

S

R

A

E

I

P

T

L

A

K

T

L

K

E

D

D

A

S

V59 (vs. gap) to L: in dbSNP:rs4008659
Y135 (≠ S112) Important for permeability to glycerol; mutation to A: Strongly decreased permeability to glycerol. Mildly decreased water permeability.
H165 (≠ A145) mutation to A: Decreased permeability to glycerol. Mildly decreased water permeability.
G264 (= G246) to V: affects water and glycerol transport; dbSNP:rs62542743

Sites not aligning to the query:

1:32 mutation Missing: Decreased interaction with PLIN1.
12 R → C: in dbSNP:rs139297434

6n1gA Crystal structure of aquaglyceroporin aqp7 (see paper)
35% identity, 87% coverage: 9:254/283 of query aligns to 1:241/249 of 6n1gA

query
sites
6n1gA

S

K

L

M

P

V

S

R

Q

E

C

F

L

L

A

A

E

E

F

F

L

M

G

S

T

T

A

Y

L

V

L

M

I

M

F

V

F

F

G

G

T

L

G

G

C

S

V

V

A

A

A

H

L

M

-

V

-

L

-

N

K

K

V

K

A

Y

G

G

A

S

S

Y

F

L

G

G

L

-

W

-

E

-

I

V

S

N

L

L

I

G

W

F

G

G

V

F

G

G

V

V

S

T

M

M

A

G

I

V

Y

H

L

V

S

A

A

G

G

R

I

I

S

S

G

G

A

A

H

H

L

M

N

N

P

A

A

A

V

V

S

T

I

F

A

A

L

N

C

C

I

A

F
x
L

T
x
G

G
x
R

F

V

E

P

K

W

R

R

K

K

L

F

P

P

F

V

Y

Y

M

V

L

L

S

G

Q

Q

V

F

A

L

G

G

A

S

F

F

C

L

G

A

A

A

F

A

L

T

V

I

Y

Y

T

S

L

L

Y

F

S

Y

N

T

L

A

F

I

F

L

D

H

F

F

E

S

Q

G

S

G

R

Q

H

L

M

M

I

V

R

T

G

G

T

P

Q

V

A

A

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

A

T

T

Y

Y

P

L

H

P

A

D

A

H

L

M

S

T

T

L

G

W

Q

R

A

G

F

F

L

L

V

N

E

E

V

A

I

W

I

L

T

T

A

G

I

M

L

L

M

Q

G

L

V

C

I

L

M
x
F

S

A

L

I

T

T

D

D

D

Q

N

E

N

N

G

N

L

P

P

A

R

L

G

P

P

G

L

T

A

E

P

A

L

L

L

V

I

I

G

G

L

I

L

L

I

V

A

V

V

I

I

I

G

G

S

V

S

S

M

L

G

G

P

M

L

N

T

T

G

G

F

Y

A

A

M

I

N

N

P

P

A

S

R

R

D

D

F

L

G

P

P

P

K

R

L

I

M

F

T

T

F

F

F

I

T

A

G

G

W

W

G

G

E

K

I

Q

S

V

L

F

T

S

G

N

G

G

R

E

D

N

I

-

P

-

Y

W

F

W

L

W

V

V

P

P

V

V

F

V

A

A

P

P

I

L

V

L

G

G

A

A

C

Y

L

L

G

G

A

G

A

I

Y

Y

binding glycerol: L73 (≠ F81), G74 (≠ T82), R75 (≠ G83), F156 (≠ M167)

I1CR68 Aquaporin-1 from Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar) (see paper)
39% identity, 84% coverage: 13:250/283 of query aligns to 60:292/306 of I1CR68

query
sites
I1CR68

Q

E

C

F

L

L

A

A

E

E

F

F

L

I

G

G

T

T

A

V

L

I

L

L

I

V

F

L

G

T

T

C

G

G

C

F

V

C

A

A

A

E

L

Q

K

T

V

L

A

H

G

I

A

E

S

E

F

S

G

K

L

S

W

W

E

L

I

T

S

S

L

S

I

F

-

G

W

S

G

G

V

L

G

S

V

V

S

L

M

I

A

G

I

I

Y

C

L

V

S

S

A

G

G

H

I

V

S

S

G

G

A

A

H

H

L

L

N

N

P

P

A

A

V

V

S

T

I

I

A

A

L

F

C

C

I

I

F

F

T

S

G

G

F

F

E

P

K

I

R

R

K

K

L

V

P

P

F

S

Y

Y

M

I

L

T

S

A

Q

Q

V

L

A

L

G

G

A

A

F

F

C

A

G

G

A

A

F

A

L

L

V

L

Y

Y

T

I

L

I

Y

I

S

E

N

P

L

A

F

I

F

V

D

Q

F

F

E

D

Q

G

S

G

R

Q

H

R

M

Y

I

I

R

L

G

G

T

E

Q

K

A

S

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

G

T

T

Y

Y

P

P

H

P

A

L

A

Y

L

V

S

G

T

I

G

G

Q

S

A

A

F

I

L

A

V

S

E

E

V

I

I

M

I

G

T

T

A

A

I

M

L

L

M

L

G

L

V

V

I

I

M

M

S

-

L

V

T

T

D

G

D

H

N

P

N

N

G

N

L

L

P

P

R

Y

G

K

P

S

L

A

A

Q

P

G

L

A

L

M

I

I

G

A

L

L

L

G

I

I

A

T

V

T

I

I

G

S

S

L

S

C

M

I

G

G

P

Y

L

T

T

S

G

G

F

F

A

S

M

L

N

N

P

P

A

A

R

R

D

D

F

F

G

G

P

P

K

R

L

L

M

F

T

T

F

A

F

I

T

A

G

G

W

W

G

-

E

-

I

-

S

-

L

-

T

-

G

-

G

G

R

F

D

D

I

V

-

F

-

K

-

V

-

Y

-
x
H

P

Y

Y

Y

F

A

L

L

V

V

P

P

V

M

F

F

A

A

P

P

I

I

V

L

G

G

A

G

C

L

L

V

G

G

H275 (vs. gap) mutation to A: Affects pH sensing; when associated with A-85.

3c02A X-ray structure of the aquaglyceroporin from plasmodium falciparum (see paper)
35% identity, 89% coverage: 9:259/283 of query aligns to 1:236/242 of 3c02A

query
sites
3c02A

S

S

L

Y

P

V

S

R

Q

E

C

F

L

I

A

G

E

E

F

F

L

L

G

G

T

T

A

F

L

V

L
|
L

I

M

F

F

F

L

G

G

T

E

G

G

C

A

V

T

A

A

A

N

L
x
F

K

H

V

T

A

T

G

G

A

L

S

S

F

G

G

D

L
x
W

W

Y

E

K

I

L

S

C

L

L

I

G

W
|
W

G

G

V

L

G

A

V
|
V

S

F

M

F

A

G

I
|
I

Y

L

L

V

S

S

A

A

G

K

I

L

S

S

G
|
G

A
|
A

H
|
H

L
|
L

N
|
N

P

L

A

A

V

V

S

S

I

I

A

G

L
|
L

C

S

I

S

F

I

T

N

G

K

F

F

E

D

K

L

R

K

K

K

L

I

P

P

F

V

Y

Y

M

F

L

F

S

A

Q

Q

V

L

A

L

G

G

A

A

F

F

C

V

G

G

A

T

F

S

L

T

V

V

Y

Y

T

G

L

L

Y

Y

S

-

N

-

L

-

F

-

D

-

F

-

E

-

Q

-

S

-

R

-

H

H

M

G

I

F

R

I

G

S

T

N

Q

S

A

K

S

I

L

P

E

Q

L

F

A

A

S

-

V

-

F

W

S

E

T

T

Y
x
S

P

R

H
x
N

A
x
P

A

S

L

I

S

S

T

L

G

T

Q

G

A

A

F

F

L

F

V

N

E

E

V

L

I

I

I

L

T

T

A

G

I

I

L
|
L

M

L

G

L

V

V

I
|
I

M

L

S

V

L

V

T
x
V

D

D

D

E

N

N

N

-

G

-

L

-

P

I

R

C

G

G

P

K

L

F

A

H

P

I

L

L

-

K

-

L

-

S

L

V

I

V

G

G

L

L

L

I

I
|
I

A

L

V

C

I

I

G

G

S

I

S

T

M

F

G

G

P
x
G

L

N

T
|
T

G
|
G

F
|
F

A
|
A

M

L

N
|
N

P

P

A

S

R
|
R

D

D

F

L

G

G

P

S

K

R

L

F

M

L

T

S

F

-

F

L

T

I

G

A

W

Y

G

G

E

K

I

D

S

T

L

F

T

T

G

-

R

K

D

D

I

N

P

F

Y

Y

F

F

L

W

V

V

P

P

V

L

F

V

A

A

P

P

I

C

V

V

G

G

A

S

C

V

L

V

G

F

A

C

A

Q

A

F

Y

Y

R

D

G

K

L

V

I

I

A

C

binding glycerol: L16 (= L24), F27 (≠ L35), W36 (≠ L44), W43 (= W51), V47 (= V55), I51 (= I59), G59 (= G67), A60 (= A68), H61 (= H69), H61 (= H69), L62 (= L70), N63 (= N71), L70 (= L78), S120 (≠ Y141), N122 (≠ H143), P123 (≠ A144), L141 (= L162), I145 (= I166), V149 (≠ T170), I170 (= I190), G179 (≠ P199), G179 (≠ P199), T181 (= T201), G182 (= G202), G182 (= G202), F183 (= F203), A184 (= A204), N186 (= N206), R189 (= R209)

2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
34% identity, 88% coverage: 9:257/283 of query aligns to 3:243/245 of 2evuA

query
sites
2evuA

S

S

L

L

P

T

S

K

Q

R

C

C

L

I

A

A

E

E

F

F

L

I

G

G

T

T

A

F

L

I

L
|
L

I

V

F

F

G

G

T

A

G

G

C

S

V

-

A
|
A

A

A

L

V

K

T

V

L

A

M

G

I

A
|
A

S
|
S

F

G

G
|
G

L

T

-
x
S

-

P

-

N

-

P

-

F

-
x
N

-
x
I

-
x
G

-
x
I

-

G

-

L

-

G

-

L

-

G

-

D

W
|
W

-

V

E

A

I

I

S

G

L

L

I

A

W

F

G

G

V

F

G

A

V
x
I

S

A

M

A

A

S

I

I

Y

Y

L

A

S

L

A

G

G

N

I

I

S

S

G
|
G

A
x
C

H
|
H

L
x
I

N
|
N

P

P

A

A

V

V

S

T

I

I

A

G

L
|
L

C

W

I

S

F

V

T

K

G

K

F

F

E

P

K

G

R

R

K

E

L

V

P

V

F

P

Y

Y

M

I

L

I

S

A

Q

Q

V

L

A

L

G

G

A

A

F

A

C

F

G

G

A

S

F

F

L

I

V

-

Y

-

T

-

L

-

Y

-

S

-

N

-

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binding glycerol: L18 (= L24), A26 (= A33), A33 (= A40), S34 (= S41), G36 (= G43), S38 (vs. gap), N43 (vs. gap), I44 (vs. gap), I44 (vs. gap), G45 (vs. gap), I46 (vs. gap), W55 (= W45), I66 (≠ V55), G78 (= G67), C79 (≠ A68), H80 (= H69), I81 (≠ L70), N82 (= N71), L89 (= L78), T128 (= T128), G131 (≠ S131), G133 (≠ E133), A134 (≠ S139), T135 (= T140), A136 (≠ Y141), P137 (= P142), F138 (≠ H143), P139 (≠ A144), L157 (= L162), I161 (= I166), A165 (≠ T170), I179 (= I186), G195 (= G202), N199 (= N206), R202 (= R209)

P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 11 papers)
31% identity, 94% coverage: 16:281/283 of query aligns to 15:249/271 of P41181

query
sites
P41181

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G64 (= G67) to R: in NDI2; loss of water channel activity; dbSNP:rs104894326
S148 (≠ L169) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: Retained in the endoplasmic reticulum.
R187 (= R209) to C: in NDI2; loss of water channel activity; mutant protein does not fold properly; dbSNP:rs104894328
A190 (≠ G212) to T: in NDI2; mutant protein does not fold properly and is not functional; dbSNP:rs104894341
V194 (≠ M216) to I: in dbSNP:rs772051028
S216 (≠ A251) to P: in NDI2; loss of water channel activity; dbSNP:rs104894329
S229 (≠ H261) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
S231 (≠ P263) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
T244 (≠ I276) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to E: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.

Sites not aligning to the query:

254 R → L: in NDI2; results in the loss of arginine vasopressin-mediated phosphorylation at S-256; R → Q: in NDI2; exerts a dominant-negative effect on wild-type-AQP2 in that it interferes with its trafficking to the apical membrane; is a loss of function instead of a gain of function mutation on dominant nephrogenic diabetes insipidus
256 modified: Phosphoserine; by PKA; S→A: Retained in vesicles.; S→D: Expressed in the apical membrane.
258 E → K: in NDI2; retained in the Golgi compartment; dbSNP:rs104894332
262 P → L: in NDI2; mutant protein folds properly and is functional but is retained in intracellular vesicles; able to assemble into tetramers with wild-type AQP2 that properly localize to the apical membrane; dbSNP:rs104894339; P→A: No effect on expression at the apical cell membrane.

P56402 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Mus musculus (Mouse) (see 2 papers)
30% identity, 98% coverage: 5:281/283 of query aligns to 4:249/271 of P56402

query
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P56402

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T126 (≠ S147) mutation to M: Does not cause loss of water channel activity, but impairs trafficking from cytoplasmic vesicles to the cell membrane.

Sites not aligning to the query:

256 modified: Phosphoserine; S → L: in cph; loss of a phosphorylation site and loss of trafficking to the apical cell membrane; causes aberrant location at the basolateral cell membrane

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
30% identity, 95% coverage: 12:279/283 of query aligns to 38:267/271 of P08995

query
sites
P08995

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S262 (≠ P274) modified: Phosphoserine; by CPK

O94778 Aquaporin-8; AQP-8 from Homo sapiens (Human) (see 4 papers)
33% identity, 72% coverage: 14:216/283 of query aligns to 38:220/261 of O94778

query
sites
O94778

C
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L

F

L

S

I

I

G

G

L

F

L

A

I

V

A

T

V

V

I

D

G

I

S

L

S

A

M

G

G

G

P

P

L

V

T

S

G

G

F

G

A
x
C

M

M

N

N

P

P

A

A

R
|
R

D

A

F

F

G

G

P

P

K

A

L

V

M

V

C38 (= C14) mutation to S: Does not affect loss of hydrogen peroxide transport after stress.
F48 (≠ L24) mutation to A: Loss of hydrogen peroxide transport activity under stress condition.
C53 (= C31) modified: Cysteine persulfide; modified: Cysteine sulfenic acid (-SOH); mutation to S: Does not affect hydrogen peroxide transport under stress condition.
H72 (≠ W51) mutation to A: Does not affect hydrogen peroxide transport activity under stress condition.
C173 (≠ I166) mutation to A: Loss of hydrogen peroxide transporter activity.; mutation to S: Slightly affect hydrogen peroxide transport after stress.
C208 (≠ A204) mutation to S: Does not affect loss of hydrogen peroxide transport after stress.
R213 (= R209) mutation to A: Does not affect hydrogen peroxide transport activity under stress condition.

Sites not aligning to the query:

8 C→S: Does not affect loss of hydrogen peroxide transport after stress.
229 I → M: in a breast cancer sample; somatic mutation
247 C→S: Does not affect loss of hydrogen peroxide transport after stress.

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
29% identity, 85% coverage: 8:247/283 of query aligns to 45:251/298 of Q6Z2T3

query
sites
Q6Z2T3

P

P

S

H

L

L

P

L

S

K

Q

K

C

V

L

V

A

S

E

E

F

V

L

V

G

A

T

T

A

F

L

L

I

V

F

F

F

M

G

T

T

C

G

G

C

A

V

A

A

G

A

I

L

S

K

G

V

S

A

D

G

L

A

S

S

R

F

I

G

S

L

Q

W

L

E

G

I

Q

S

S

L

I

I

A

W

G

G

G

V

L

G

I

V

V

S

T

M

V

A

M

I

I

Y

Y

L

A

S

V

A

G

G

H

I

I

S

S

G

G

A

A

H

H

L

M

N

N

P

P

A

A

V

V

S

T

I

L

A

A

L

F

C

A

I

V

F

F

T

R

G

H

F

F

E

P

K

W

R

I

K

Q

L

V

P

P

F

F

Y

Y

M

W

L

A

S
x
A

Q

Q

V

F

A

T

G

G

A

A

F

I

C

C

G

A

A

S

F

F

L

V

V

L

Y

-

T

-

L

-

Y

-

S

-

N

-

L

-

F

-

D

-

F

-

E

-

Q

-

S

-

R

-

H

-

M

-

I

-

R

-

G

-

T

-

Q

K

A

A

S

V

L

I

E

H

L

P

A

V

S

D

V

V

F

I

S

G

T

T

Y

T

-

T

-

P

-

V

-

G

P

P

H

H

A

-

L

-

S

-

T

-

G

W

Q

H

A

S

F

L

L

V

V

V

E

E

V

V

I

I

I

V

T

T

A

F

I

N

L

M

M

M

G

F

V

V

I

T

M

L

S

A

L

V

T

A

D

T

D

D

N

T

N

R

G

A

L

V

P

-

R

-

G

G

P

E

L

L

A

A

P

G

L

L

L

A

I

V

G

G

L

S

L

A

I

V

A

C

V

I

I

T

G

S

S

I

S

F

M

A

G

G

P

A

L

I

T

S

G

G

F

G

A

S

M

M

N

N

P

P

A

A

R

R

D

T

F

L

G

G

P

P

K

A

L

L

M

A

T

S

F

N

-

K

F

F

T

D

G

G

-

L

W

W

G

-

E

-

I

-

S

-

L

-

T

-

G

-

R

-

D

-

I

-

P

I

Y

Y

F

F

L

L

V

G

P

P

V

V

F

M

A

G

P

T

I

L

V

S

G

G

A

A

A132 (≠ S95) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

4nefA X-ray structure of human aquaporin 2 (see paper)
31% identity, 89% coverage: 3:255/283 of query aligns to 1:219/239 of 4nefA

query
sites
4nefA

T

S

A

E

L

L

Q

R

Q

S

P

I

S

A

L

F

P

S

S

R

Q

A

C

V

L

F

A

A

E

E

F

F

L

L

G

A

T

T

A

L

L

L

L

F

I

V

F

F

G

G

T

L

G

G

C

-

V

-

A

S

A

A

L

L

K

N

V

W

A

P

G

Q

A

A

S

L

F

P

G

S

L

V

W

L

E

Q

I

I

S

A

L

M

I

A

W

F

G

G

V

L

G

G

V

I

S

G

M

T

A

L

I

V

Y

Q

L

A

S

L

A

G

G

H

I

I

S

S

G

G

A

A

H

H

L

I

N

N

P

P

A

A

V

V

S

T

I

V

A

A

L
x
C

C

L

I

V

F

G

T

C

G
x
H

F

V

E

S

K

V

R

L

K

R

L

A

P

A

F

F

Y

Y

M

V

L

A

S

A

Q

Q

V

L

A

L

G

G

A

A

F

V

C

A

G

G

A

A

F

A

L

L

V

L

Y

H

T

-

L

-

Y

-

S

-

N

-

L

-

F

-

D

-

F

-

E

E

Q

I

S

T

R

P

H

A

M

D

I

I

R

R

G

G

T

D

Q

L

A

A

S

V

L

N

E

A

L

L

A

S

S

N

V

-

F

-

S

-

T

-

Y

-

P

-

H

-

A

-

A

S

L

T

S

T

T

A

G

G

Q

Q

A

A

F

V

L

T

V

V

E

E

V

L

I

F

I

L

T

T

A

L

I

Q

L

L

M

V

G

L

V

C

I

I

M

F

S

A

L

S

T

T

D

D

D

E

N

R

G

G

L

-

P

-

R

E

G

N

P

P

L

G

A

T

P

P

L

A

L

L

-

S

I

I

G

G

L

F

L

S

I

V

A

A

V

-

I

L

G

G

S

H

S

L

M

L

G

G

-

I

P

H

L

Y

T

T

G

G

F

C

A

S

M

M

N

N

P

P

A

A

R

R

D

S

F

L

G

A

P

P

K

A

L

V

M

V

T

T

-

G

-

K

F

F

F

D

T

D

G

H

W

W

G

-

E

-

I

-

S

-

L

-

T

-

G

-

R

-

D

-

I

-

P

-

Y

-

F

-

L

-

V

V

P

F

V

W

F

I

A

G

P

P

I

L

V

V

G

G

A

A

C

I

L

L

G

G

A

S

A

L

Y

Y

R

N

binding zinc ion: C74 (≠ L78), H79 (≠ G83)

Q9SAI4 Aquaporin NIP6-1; NOD26-like intrinsic protein 6-1; AtNIP6;1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 90% coverage: 1:254/283 of query aligns to 67:285/305 of Q9SAI4

query
sites
Q9SAI4

M

V

T

T

T

C

A

S

L

L

Q

P

P

P

-

N

-

V

S

S

L

L

P

Y

S

R

Q

K

C

L

L

G

A

A

E

E

F

F

L

V

G

G

T

T

A

L

L

I

L

L

I

I

F

F

F

A

G

G

T

T

G

A

C

T

V

A

A

I

A

V

L

N

K

Q

V

K

A

T

G

D

A

G

S

A

F

E

G

T

L

L

W

I

E

G

I

C

S

A

L

A

I

S

W
x
A

G

G

V

L

G

A

V

V

S

M

M

I

A

V

I

I

Y

L

L

S

S

T

A

G

G

H

I

I

S

S

G

G

A

A

H

H

L

L

N

N

P

P

A

A

V

V

S

T

I

I

A

A

L

F

C

A

I

A

F

L

T

K

G

H

F

F

E

P

K

W

R

K

K

H

L

V

P

P

F

V

Y

Y

M

I

L

G

S

A

Q

Q

V

V

A

M

G

A

A

S

F

V

C

S

G

A

A

A

F

F

L

A

V

L

Y

K

T

A

L

V

Y

-

S

-

N

-

L

-

F

-

D

-

F

F

E

E

Q

P

S

T

R

-

H

-

M

M

I

S

R

G

G

G

T

V

Q

-