SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_1122 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1122 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P46154 Glutathione-independent formaldehyde dehydrogenase; FALDH; FDH; Formaldehyde dismutase; EC 1.2.1.46; EC 1.2.98.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
93% identity, 100% coverage: 1:399/399 of query aligns to 1:399/399 of P46154

query
sites
P46154

M

M

S

S

G

G

N

N

R

R

G

G

V

V

Y

Y

L

L

G

G

A

S

G

G

K

K

V

V

E

E

V

V

Q

Q

K

K

I

I

D

D

Y

Y

P

P

K

K

M

M

Q

Q

D

D

P

P

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R

G

G

R

K

K

K

I

I

N

E

H

H

A

G

V

V

I

I

L

L

R

K

V

V

S

S

T

T

N

N

I

I

C
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C

G
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G

S
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S

D
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D

Q
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Q

H
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H

M

M

V

V

R

R

G

G

R

R

T

T

A

A

Q

Q

T

V

G

G

L

L

V

V

L

L

G

G

H
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H

E

E

I

I

T

T

G

G

E

E

V

V

I

I

E

E

K

K

G

G

S

R

D

D

V

V

E

E

N

N

L

L

Q

Q

I

I

G

G

D

D

L

L

V

V

S

S

V

V

P

P

F

F

N

N

V

V

A

A

C
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C

G

G

R

R

C
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C

R

R

S

S

C
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C

K

K

E

E

M

M

H

H

T

T

G

G

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V

C
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C

L

L

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T

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V

N

N

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P

A

A

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R

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A

G

G

A

A

Y

Y

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G

Y

Y

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V

D

D

M

M

G

G

D

D

W

W

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T

G

G

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Q

A

A

E

E

Y

Y

A

L

M

L

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V

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P

Y

Y

A

A

D

D

F

F

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N

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L

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K

L

L

P

P

D

D

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R

D

D

R

K

A

A

M

M

E

E

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K

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D

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L

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T

C

C

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L

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S

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D

I

I

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L

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P

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T

G

G

Y

Y

H

H

G

G

A

A

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T

T

A

A

G

G

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V

G

G

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P

G

G

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S

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T

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V

Y

Y

I

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A

G

G

A

A

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V
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V

G

G

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L

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A

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S

A

A

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L

G

G

A

A

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I

I

I

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D

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R

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L

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A

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H

A

A

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K

A

A

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Q

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G

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F

E

E

I

I

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D

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I

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A

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G

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P

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E

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V

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D

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C

A

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A

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V

G

G

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F

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E

A

A

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R

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H
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H

G

G

H

H

D

E

G

G

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A

K

K

H

H

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E

A

A

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P

A

A

T

T

V

V

L

L

N

N

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S

L

L

M

M

G

Q

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V

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T

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R

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V

A

A

G

G

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K

I

I

G

G

I

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L
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L

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Y

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T

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E

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D

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G

A

A

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D

D

A

A

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M

I

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G

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L

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S

I

I

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R

F

F

G

G

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L

G

G

W

W

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A

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K

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S

H

H

S

S

F

F

H

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T

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G

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P

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V

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M

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K

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Y

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L

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M

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A

A

I

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M

M

W

W

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D

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I

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N

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I

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A

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E

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A

C47 (= C47) binding
GSDQH 48:52 (= GSDQH 48:52) binding
H68 (= H68) binding
C98 (= C98) binding
C101 (= C101) binding
C104 (= C104) binding
C112 (= C112) binding
V198 (= V198) binding
D218 (= D218) binding
R223 (= R223) binding
V263 (= V263) binding
RGH 268:270 (= RGH 268:270) binding
PGL 300:302 (= PGL 300:302) binding
GQT 337:339 (= GQT 337:339) binding

1kolA Crystal structure of formaldehyde dehydrogenase (see paper)
93% identity, 99% coverage: 3:398/399 of query aligns to 1:396/396 of 1kolA

query
sites
1kolA

G

G

N

N

R

R

G

G

V

V

Y

Y

L

L

G

G

A

S

G

G

K

K

V

V

E

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Q

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K

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I

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D

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Y

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P

K

K

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M

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Q

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D

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P

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R

G

G

R

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K

K

I

I

N

E

H

H

A

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V

V

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I

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L

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K

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V

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S

T

T

N

N

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I

C
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C

G
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G

S
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S

D

D

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Q

H
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H

M

M

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V

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R

G

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L

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V

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L

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E
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E

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I

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T

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G

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K

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D

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L

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Q

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I

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G

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D

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L

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V

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S

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V

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P

F
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F

N

N

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V

A

A

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C

G

G

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C
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C

R

R

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C

K

K

E

E

M

M

H

H

T

T

G

G

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V

C
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C

L

L

S

T

V

V

N
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N

P

P

A

A

R

R

P

A

G

G

A

A

Y

Y

G

G

Y

Y

V

V

D

D

M

M

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G

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D

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W

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T

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G

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Q

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A

E

E

Y

Y

A

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M

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V

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A

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D

F

F

N

N

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L

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K

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L

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P

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D

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R

D

D

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K

A

A

M

M

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E

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K

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I

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D

L

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T

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C

C

L

L

S

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D
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D

I

I

L

L

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T

G

G

Y

Y

H

H

G

G

A

A

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V

T

T

A

A

G

G

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G

G

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P

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I

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A

G
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G

A

A

G
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G

P
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P

V
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G

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L

L

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A

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R

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L

G

G

A

A

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V

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I

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G

D
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D

V
x
L

N

N

T

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V

A

R
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R

L

L

A

A

H

H

A

A

K

K

A

A

Q

Q

G

G

F

F

E

E

I

I

A

A

D

D

L
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L

S

S

L

L

D

D

T

T

P

P

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L

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H

E

E

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Q

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I

A

A

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L

G

G

E

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D

D

C

C

A

A

I

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D

D

A
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A

V
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V

G

G

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F

E

E

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R
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R

G

G

H
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H

G

G

H

H

D

E

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G

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A

K

K

H

H

E

E

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A

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P

A

A

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V

L

L

N

N

S

S

L

L

M

M

G

Q

V

V

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T

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R

V

V

A

A

G

G

K

K

I

I

G

G

I

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P
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P

G
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G

L
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L

Y

Y

V

V

T

T

E

E

D

D

P

P

G

G

A

A

V

V

D

D

A

A

K

K

M

I

G

G

S

S

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L

S

S

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I

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R

F

F

G

G

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L

G

G

W

W

A

A

K

K

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S

H

H

S

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F

F

H

H

T

T

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G

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Q

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T

P

P

V

V

M

M

K

K

Y

Y

N

N

R

R

A

A

L

L

M

M

Q

Q

A

A

I

I

M

M

W

W

D

D

R

R

I

I

N

N

I

I

A

A

E

E

V

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G

G

V

V

Q

Q

V

V

I

I

S

S

L

L

D

D

Q

D

A

A

P

P

Q

R

G

G

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Y

G

G

E

E

F
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F

D

D

A

A

G

G

V

V

P

P

K

K

K
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K

F

F

V

V

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D

D

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P

H

H

K

K

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T

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F

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S

A

A

active site: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), H66 (= H68), E67 (= E69), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), N114 (= N116), D168 (= D170), T172 (= T174), K385 (= K387)
binding nicotinamide-adenine-dinucleotide: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), F92 (= F94), D168 (= D170), T172 (= T174), A191 (= A193), G192 (= G194), G194 (= G196), P195 (= P197), V196 (= V198), G215 (= G217), D216 (= D218), L217 (≠ V219), R221 (= R223), L235 (= L237), A260 (= A262), V261 (= V263), A265 (= A267), R266 (= R268), H268 (= H270), V281 (= V283), P298 (= P300), G299 (= G301), L300 (= L302), G335 (= G337), Q336 (= Q338), T337 (= T339), F378 (= F380)
binding zinc ion: C45 (= C47), H66 (= H68), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), D168 (= D170)

4jlwA Crystal structure of formaldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
87% identity, 99% coverage: 3:396/399 of query aligns to 1:394/395 of 4jlwA

query
sites
4jlwA

G

G

N

N

R

R

G

G

V

V

Y

Y

L

L

G

G

A

P

G

G

K

K

V

V

E

E

V

V

Q

Q

K

N

I

I

D

P

Y

Y

P

P

K

K

M

M

Q

Q

D

D

P

P

R

Q

G

G

R

R

K

Q

I

I

N

D

H

H

A

G

V

V

I

I

L

L

R

R

V

V

S

S

T

T

N

N

I

I

C
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C

G
|
G

S
|
S

D

D

Q

Q

H
|
H

M

M

V

V

R

R

G

G

R

R

T

T

A

A

Q

P

T

E

G

G

L

L

V

V

L

L

G

G

H
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H

E

E

I

I

T

T

G

G

E

E

V

V

I

V

E

E

K

I

G

G

S

R

D

G

V

V

E

E

N

T

L

M

Q

K

I

I

G

G

D

D

L

L

V

V

S

S

V

V

P

P

F
|
F

N

N

V

V

A

A

C
|
C

G

G

R

H

C
|
C

R

R

S

T

C
|
C

K

K

E

E

M

Q

H

H

T

T

G

G

V

V

C
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C

L

L

S

T

V

V

N

N

P

P

A

A

R

R

P

A

G

G

A

A

Y

Y

G

G

Y

Y

V

V

D

D

M

M

G

G

D

G

W

W

T

V

G

G

Q

Q

A

A

E

E

Y

Y

A

V

M

L

V

V

P

P

Y

Y

A

A

D

D

F

F

N

N

L

L

K

K

L

L

P

P

D

N

R

R

D

E

R

A

A

A

M

M

E

E

K

K

I

I

R

R

D

D

L

L

T

T

C

C

L

L

S

S

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D

I

I

L

L

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T
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T

G

G

Y

Y

H

H

G

G

A

A

V

V

T

T

A

A

G

G

V

V

G

G

P

P

G

G

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S

T

T

V

V

Y

Y

I

I

A

A

G
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G

A

A

G
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G

P
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P

V
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V

G

G

L

L

A

A

S

S

A

A

R

R

L

L

G

G

A

A

V

V

I

I

I

V

G
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G

D
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D

V
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V

N

N

T

P

V

T

R
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R

L

L

A

A

H

H

A

A

K

K

A

K

Q

Q

G

G

F

F

E

E

I

I

A

A

D

D

L

L

S

S

L

K

D

D

T

T

P

P

L

L

H

H

E

E

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Q

I

I

A

A

L

L

G

G

E

E

P

P

E

E

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D

D

C

C

A

A

I

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D

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A

V
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V

G

G

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F

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A

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R

G

G

H
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H

G

G

H

H

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S

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G

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S

K

Q

H

Q

E

E

A

A

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P

A

A

T

T

V
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V

L

L

N

N

S

S

L

L

M

M

G

G

V

I

V

T

R

R

V

V

A

A

G

G

K

K

I

I

G

G

I

I

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P

G
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G

L
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L

Y

Y

V

V

T

T

E

E

D

D

P

P

G

G

A

A

V

V

D

D

A

A

K

K

M

H

G

G

S

A

L

L

S

S

I

I

R

R

F

F

G

G

L

L

G

G

W

W

A

A

K

K

S

S

H

H

S

S

F

F

H

H

T

T

G

G

Q
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Q

T
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T

P

P

V

V

M

M

K

K

Y

Y

N

N

R

R

A

Q

L

L

M

M

Q

Q

A

A

I

I

M

M

W

W

D

D

R

R

I

I

N

K

I

I

A

A

E

D

V

I

V

V

G

G

V

V

Q

E

V

V

I

I

S

T

L

L

D

D

Q

D

A

A

P

P

Q

K

G

G

Y

Y

G

G

E

E

F
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F

D

D

A

A

G

G

V

V

P

P

K

K

F

F

V

V

I

I

D

D

P

P

H

H

K

N

M

L

F

F

binding nicotinamide-adenine-dinucleotide: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), F92 (= F94), D168 (= D170), T172 (= T174), G192 (= G194), G194 (= G196), P195 (= P197), V196 (= V198), G215 (= G217), D216 (= D218), V217 (= V219), R221 (= R223), A260 (= A262), V261 (= V263), A265 (= A267), R266 (= R268), H268 (= H270), V281 (= V283), P298 (= P300), G299 (= G301), L300 (= L302), Q336 (= Q338), T337 (= T339), F378 (= F380)
binding zinc ion: C45 (= C47), H66 (= H68), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), D168 (= D170)

Q52078 Formaldehyde dismutase; EC 1.2.98.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
59% identity, 99% coverage: 1:397/399 of query aligns to 1:397/399 of Q52078

query
sites
Q52078

M
|
M

S
x
A

G
|
G

N
|
N

R
x
K

G
x
S

V
|
V

Y
|
Y

L
x
H

G
|
G

A
x
T

G
x
R

K
x
D

V
x
L

E
x
R

V
|
V

Q
x
E

K
x
T

I
x
V

D
x
P

Y
|
Y

P
|
P

K
|
K

M
x
L

Q
x
E

D
x
H

P

-

R
x
N

G
x
N

R
|
R

K
|
K

I
x
L

N
x
E

H
|
H

A
|
A

V
|
V

I
|
I

L
|
L

R
x
K

V
|
V

S
|
S

T
|
T

N
|
N

I
|
I

C
|
C

G
|
G

S
|
S

D
|
D

Q
|
Q

H
|
H

M
x
I

V
x
Y

R
|
R

G
|
G

R
|
R

T
x
F

T
x
I

A
x
V

Q
x
P

T
x
K

G
|
G

L
x
H

V
|
V

L
|
L

G
|
G

H
|
H

E
|
E

I
|
I

T
|
T

G
|
G

E
|
E

V
|
V

I
x
V

E
|
E

K
|
K

G
|
G

S
|
S

D
|
D

V
|
V

E
|
E

N
x
L

L
x
M

Q
x
D

I
|
I

G
|
G

D
|
D

L
|
L

V
|
V

S
|
S

V
|
V

P
|
P

F
|
F

N
|
N

V
|
V

A
|
A

C
|
C

G
|
G

R
|
R

C
|
C

R
|
R

S
x
N

C
|
C

K
|
K

E
|
E

M
x
A

H
x
R

T
x
S

G
x
D

V
|
V

C
|
C

L
x
E

S
x
N

-
x
N

-
x
L

V
|
V

N
|
N

P
|
P

A
x
D

R
x
A

P
x
D

G
x
L

G
|
G

A
|
A

Y
x
F

G
|
G

Y
x
F

V

-

D
|
D

M
x
L

G
x
K

D
x
G

W
|
W

T
x
S

G
|
G

Q
|
Q

A
|
A

E
|
E

Y
|
Y

A
x
V

M
x
L

V
|
V

P
|
P

Y
|
Y

A
|
A

D
|
D

F
x
Y

N
x
M

L
|
L

K
|
K

L
x
F

P
x
G

D
|
D

R
x
K

D
x
E

R
x
Q

A
|
A

M
|
M

E
|
E

K
|
K

I
|
I

R
x
K

D
|
D

L
|
L

T
|
T

C
x
L

L
x
I

S
|
S

D
|
D

I
|
I

L
|
L

P
|
P

T
|
T

G
|
G

Y
x
F

H
|
H

G
|
G

A
x
C

V
|
V

T
x
S

A
|
A

G
|
G

V
|
V

G
x
K

P
|
P

G
|
G

S
|
S

T
x
H

V
|
V

Y
|
Y

I
|
I

A
|
A

G
|
G

A
|
A

G
|
G

P
|
P

V
|
V

G
|
G

L
x
R

A
x
C

A
|
A

S
x
G

A
|
A

R
|
R

L
|
L

G
|
G

A
|
A

V
x
C

V
|
V

I
|
I

I
x
V

G
|
G

D
|
D

V
x
Q

N
|
N

T
x
P

V
x
E

R
|
R

L
|
L

A
x
K

H
x
L

A
x
L

K
x
S

A
x
D

Q
x
A

G
|
G

F
|
F

E
|
E

I
x
T

A
x
I

D
|
D

L
|
L

S
x
R

L
x
N

D
x
S

T
x
A

P
|
P

L
|
L

H
x
R

E
x
D

Q
|
Q

I
|
I

A
x
D

A
x
Q

L
x
I

L
|
L

G
|
G

E
x
K

P
|
P

E
|
E

V
|
V

D
|
D

C
|
C

A
x
G

I
x
V

D
|
D

A
|
A

V
|
V

G
|
G

F
|
F

E
|
E

A
|
A

R
x
H

G
|
G

H
x
L

G
|
G

H

-

D
|
D

G
x
E

V
x
A

K
x
N

H
x
T

E
|
E

A
x
T

P
|
P

A
x
N

T
x
G

V
x
A

L
|
L

N
|
N

S
|
S

L
|
L

M
x
F

G
x
D

V
|
V

R
|
R

V
x
A

A
x
G

G
|
G

K
x
A

I
|
I

G
|
G

I
|
I

P
|
P

G
|
G

L
x
I

Y
|
Y

V
|
V

T
x
G

E
x
S

D
|
D

P
|
P

G
x
D

A
x
P

V
|
V

D
x
N

A
x
K

A
x
D

A
|
A

K
x
G

M
x
S

G
|
G

S
x
R

L
|
L

S
x
H

I
x
L

R
x
D

F
|
F

G
|
G

L
x
K

G
x
M

W
|
W

A
x
T

K
|
K

S
|
S

H
x
I

S
x
R

F
x
I

H
x
M

T
|
T

G
|
G

Q
x
M

T
x
A

P
|
P

V
|
V

M
x
T

K
x
N

Y
|
Y

N
|
N

R
|
R

A
x
H

L
|
L

M
x
T

Q
x
E

A
|
A

I
|
I

M
x
L

W
|
W

D
|
D

R
x
Q

I
x
M

-
x
P

N
x
Y

I
x
L

A
x
S

E
x
K

V
|
V

V
x
M

G
x
N

V
x
I

Q
x
E

V
|
V

I
|
I

S
x
T

L
|
L

D
|
D

Q
|
Q

A
|
A

P
|
P

Q
x
D

G
|
G

Y
|
Y

G
x
A

E
x
K

F
|
F

D
|
D

A
x
K

G
|
G

V
x
S

P
|
P

K
x
A

K
|
K

F
|
F

V
|
V

I
|
I

D
|
D

P
|
P

H
|
H

K
x
G

M
|
M

F
x
L

S
x
K

M1 (= M1) modified: Initiator methionine, Removed
C46 (= C47) binding
GSDQH 47:51 (= GSDQH 48:52) binding
H67 (= H68) binding
C97 (= C98) binding
C100 (= C101) binding
C103 (= C104) binding
C111 (= C112) binding
D170 (= D170) binding
T174 (= T174) binding
PV 197:198 (= PV 197:198) binding
DQ 218:219 (≠ DV 218:219) binding
R223 (= R223) binding
V263 (= V263) binding
H268 (≠ R268) binding
P299 (= P300) binding
PGI 299:301 (≠ PGL 300:302) binding
GMA 336:338 (≠ GQT 337:339) binding

Sites not aligning to the query:

2:399 modified: mature protein, Formaldehyde dismutase

2dphA Crystal structure of formaldehyde dismutase
59% identity, 99% coverage: 2:397/399 of query aligns to 1:396/398 of 2dphA

query
sites
2dphA

S

A

G

G

N

N

R

K

G

S

V

V

Y

Y

L

H

G

G

A

T

G

R

K

D

V

L

E

R

V

V

Q

E

K

T

I

V

D

P

Y

Y

P

P

K

K

M

L

Q

E

D

H

P

-

R

N

G

N

R

R

K

K

I

L

N

E

H

H

A

A

V

V

I

I

L

L

R

K

V

V

S

S

T

T

N

N

I

I

C
|
C

G
|
G

S
|
S

D

D

Q

Q

H
|
H

M

I

V

Y

R

R

G

G

R

R

T

F

T

I

A

V

Q

P

T

K

G

G

L

H

V

V

L

L

G

G

H
|
H

E
|
E

I

I

T

T

G

G

E

E

V

V

I

V

E

E

K

K

G

G

S

S

D

D

V

V

E

E

N

L

L

M

Q

D

I

I

G

G

D

D

L

L

V

V

S

S

V

V

P

P

F
|
F

N

N

V

V

A

A

C
|
C

G

G

R

R

C
|
C

R

R

S

N

C
|
C

K

K

E

E

M

A

H

R

T

S

G

D

V

V

C
|
C

L

E

S

N

-

N

-
x
L

V

V

N

N

P

P

A

D

R

A

P

D

G

L

G

G

A

A

Y

F

G

G

Y

F

V

-

D

D

M

L

G

K

D

G

W

W

T

S

G

G

Q

Q

A

A

E

E

Y

Y

A

V

M

L

V

V

P

P

Y

Y

A

A

D

D

F

Y

N

M

L

L

K

K

L

F

P

G

D

D

R

K

D

E

R

Q

A

A

M

M

E

E

K

K

I

I

R

K

D

D

L

L

T

T

C

L

L

I

S
|
S

D
|
D

I

I

L

L

P
|
P

T
|
T

G

G

Y

F

H

H

G

G

A

C

V

V

T

S

A

A

G

G

V

V

G

K

P

P

G

G

S

S

T

H

V

V

Y

Y

I

I

A
|
A

G
|
G

A

A

G
|
G

P
|
P

V
|
V

G

G

L

R

A

C

A

A

S

G

A

A

R

R

L

L

G

G

A

A

V

C

V

V

I

I

I

V

G
|
G

D
|
D

V
x
Q

N

N

T

P

V

E

R
|
R

L

L

A

K

H

L

A

L

K

S

A

D

Q

A

G

G

F

F

E

E

I

T

A

I

D

D

L
|
L

S

R

L

N

D

S

T

A

P

P

L

L

H

R

E

D

Q

Q

I

I

A

D

A

Q

L

I

L

L

G

G

E

K

P

P

E

E

V

V

D

D

C

C

A

G

I

V

D

D

A
|
A

V
|
V

G
|
G

F

F

E

E

A
|
A

R
x
H

G

G

H

L

G

G

H

-

D

D

G

E

V

A

K

N

H

T

E

E

A

T

P

P

A

N

T
x
G

V
x
A

L

L

N

N

S

S

L

L

M

F

G

D

V

V

R

R

V

A

A

G

G

G

K

A

I

I

G

G

I

I

P
|
P

G
|
G

L
x
I

Y

Y

V

V

T

G

E

S

D

D

P

P

G

D

A

P

V

V

D

N

A

K

A

D

A

A

K

G

M

S

G

G

S

R

L

L

S

H

I

L

R

D

F

F

G

G

L

K

G

M

W

W

A

T

K

K

S

S

H

I

S

R

F

I

H

M

T

T

G
|
G

Q
x
M

T
x
A

P

P

V

V

M

T

K

N

Y

Y

N

N

R

R

A

H

L

L

M

T

Q

E

A

A

I

I

M

L

W

W

D

D

R

Q

I

M

-

P

N

Y

I

L

A

S

E

K

V

V

V

M

G

N

V

I

Q

E

V

V

I

I

S

T

L

L

D

D

Q

Q

A

A

P

P

Q

D

G

G

Y

Y

G

A

E

K

F
|
F

D

D

A

K

G

G

V

S

P

P

K

A

K
|
K

F

F

V

V

I

I

D

D

P

P

H

H

K

G

M

M

F

L

S

K

active site: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), H66 (= H68), E67 (= E69), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), L114 (vs. gap), S168 (= S169), D169 (= D170), P172 (= P173), K386 (= K387)
binding nicotinamide-adenine-dinucleotide: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), F92 (= F94), D169 (= D170), T173 (= T174), A192 (= A193), G193 (= G194), G195 (= G196), P196 (= P197), V197 (= V198), G216 (= G217), D217 (= D218), Q218 (≠ V219), R222 (= R223), L236 (= L237), A261 (= A262), V262 (= V263), G263 (= G264), A266 (= A267), H267 (≠ R268), G280 (≠ T282), A281 (≠ V283), P298 (= P300), G299 (= G301), I300 (≠ L302), G335 (= G337), M336 (≠ Q338), A337 (≠ T339), F379 (= F380)
binding zinc ion: C45 (= C47), H66 (= H68), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), D169 (= D170)

4cpdA Alcohol dehydrogenase tadh from thermus sp. Atn1
30% identity, 94% coverage: 5:381/399 of query aligns to 2:334/346 of 4cpdA

query
sites
4cpdA

R

R

G

A

V

V

Y

F

L

E

G

N

A

K

G

E

K

R

V

V

E

A

V

V

Q

K

K

E

I

V

D

N

Y

A

P

P

K

R

M

L

Q

Q

D

H

P

P

R

L

G

D

R

-

K

-

I

-

N

-

H

-

A

-

V

A

I

L

L

V

R

R

V

V

V

H

S

L

T

A

N

G

I

I

C
|
C

G
|
G

S
|
S

D

D

Q

L

H
|
H

M

L

V

Y

R

H

G

G

R

K

T

I

T

P

A

V

Q

L

T

P

G

G

L

S

V

V

L

L

G

G

H
|
H

E
|
E

I

F

T

V

G

G

E

Q

V

V

I

E

E

A

K

V

G

G

S

E

D

G

V

I

E

Q

N

D

L

L

Q

Q

I

P

G

G

D

D

L

W

V

V

S

V

V

G

P

P

F
|
F

N

H

V

I

A

A

C
|
C

G

G

R

T

C
|
C

R

P

S

Y

C
|
C

K

R

E

R

M

H

H

Q

T

Y

G

N

V

L

C
|
C

L

-

S

-

V

-

N

-

P

-

A

-

R

E

P

R

G

G

G
|
G

A

V

Y

Y

G

G

Y

Y

V

G

D

P

M

M

-

F

G

G

D

N

W

L

T

Q

G

G

G

A

Q

Q

A

A

E

E

Y

I

A

L

M

R

V

V

P

P

Y

F

A

S

D

N

F

V

N

N

L

L

L

R

K

K

L

L

P

P

D

P

R

N

D

L

R

S

A

P

M

E

E

R

K

A

I

I

R

-

D

-

L

-

T

-

C

F

L

A

S

G

D
|
D

I

I

L

L

P

S

T
|
T

G

A

Y

Y

H

G

G

G

A

L

V

I

T

Q

A

G

G

Q

V

L

G

R

P

P

G

G

S

D

T

S

V

V

Y

A

I

V

A

I

G

G

A

A

G
|
G

P
|
P

V
|
V

G

G

L

L

A

M

A

A

A

I

A

E

S

V

A

A

R

Q

L

V

L

L

G

G

A

A

A

S

V

K

V

I

I

L

I

A

G

I

D
|
D

V
x
R

N
x
I

T

P

V

E

R
|
R

L

L

A

E

H

R

A

A

K

A

A

S

Q

L

G

G

F

A

E

I

I

P

A

I

D

N

L

A

S

E

L

Q

D

E

T

N

P

P

L

V

H

R

E

-

Q

R

I

V

A

R

A

S

L

E

L

T

G

N

E

D

P

E

E

G

V

P

D

D

C

L

A

V

I

L

D

E

A
|
A

V
|
V

G

G

F

G

E

-

A

-

R

-

G

-

H

-

G

-

H

-

D

-

G

-

V

-

K

-

H

-

E

-

A

-

P

-

A

A

T

A

V
x
T

L

L

N

S

S

L

L

A

M

L

G

E

V

M

V

V

R

R

V

P

A

G

G

G

K

R

I

V

G

S

I

A

P
x
V

G
|
G

L

-

Y

-

V

-

T

-

E

-

D

-

P

-

G

-

A

-

V
|
V

D

D

A

N

A

A

A

P

K

-

M

-

G

-

S

S

L

F

S

P

I

F

R

P

F

L

G

A

L

S

G

G

W

L

A

V

K

K

S

D

H

L

S

T

F

F

H

R

T

I

G
|
G

Q
x
L

T
x
A

P

N

V

V

M

H

K

L

Y

Y

N

I

R

D

A

A

L

V

M

L

Q

A

A

L

I

L

M

A

W

S

D

G

R

R

I

L

N

Q

I

P

A

E

E

R

V

I

V

V

G

S

V

-

Q

H

V

Y

I

L

S

P

L

L

D

E

Q

E

A

A

P

P

Q

R

G

G

Y

Y

G

E

E

L

F
|
F

D

D

active site: C38 (= C47), G39 (= G48), S40 (= S49), H43 (= H52), H59 (= H68), E60 (= E69), C89 (= C98), C92 (= C101), C95 (= C104), C103 (= C112), G107 (= G122), D152 (= D170), T156 (= T174)
binding nicotinamide-adenine-dinucleotide: G39 (= G48), S40 (= S49), H43 (= H52), F85 (= F94), D152 (= D170), T156 (= T174), G178 (= G196), P179 (= P197), V180 (= V198), D200 (= D218), R201 (≠ V219), I202 (≠ N220), R205 (= R223), A243 (= A262), V244 (= V263), T249 (≠ V283), V266 (≠ P300), G267 (= G301), V268 (= V311), G291 (= G337), L292 (≠ Q338), A293 (≠ T339), F333 (= F380)
binding zinc ion: C38 (= C47), H59 (= H68), C89 (= C98), C92 (= C101), C95 (= C104), C103 (= C112), D152 (= D170)

Sites not aligning to the query:

active site: 340

5ylnA Zinc dependent alcohol dehydrogenase 2 from streptococcus pneumonia - apo form
35% identity, 67% coverage: 1:267/399 of query aligns to 2:246/348 of 5ylnA

query
sites
5ylnA

M

M

S

G

G

S

N

M

R

K

G

A

V

Y

V

T

Y

Y

L

V

G

K

A

P

G

G

K

L

V

A

E

S

V

F

Q

V

K

D

I

V

D

D

Y

K

P

P

K

V

M

I

Q

R

D

K

P

P

R

T

G

A

R

-

K

-

I

-

N

-

H

-

A

-

V

-

I

I

L

V

R

R

V

I

V

V

S

K

T

T

N

T

I

I

C
|
C

G

G

S
x
T

D

D

Q

L

H
|
H

M

I

V

I

R

K

G

G

R

D

T

V

-

P

T

T

A

C

Q

Q

T

S

G

G

L

T

V

I

L

L

G

G

H
|
H

E

E

I

G

T

I

G

G

E

I

V

V

I

E

E

E

K

V

G

G

S

E

D

G

V

V

E

S

N

N

L

F

Q

K

I

K

G

G

D

D

L

K

V

V

S

L

V

I

P

S

F

C

N

V

V

C

A

A

C
|
C

G

G

R

K

C
|
C

R

Y

S

Y

C
|
C

K

K

E

K

M

-

H

-

T

-

G

-

V

-

C

-

L

-

S

-

V

-

N

-

P

-

A

-

R

-

P

-

G

-

G

G

A

I

Y

Y

G

A

Y

H

V
x
C

-

E

D

D

M

E

G

G

D

G

W

W

T

I

-

F

-

I

-

D

G

G

G

M

Q

Q

A

A

E

E

Y

Y

A

L

M

R

V

V

P

P

Y

H

A

A

D

D

F

N

N

T

L

L

L

Y

K

H

L

T

P

P

D

-

R

-

D

-

R

-

A

-

M

-

E

E

K

D

I

L

R

S

D

D

L

-

T

E

C

A

L

L

S

V

D

M

I

L

L

L

P

P

T

T

G

G

Y

Y

H

E

-

I

G

G

A

V

V

L

T

K

A

G

G

K

V

V

G

E

P

P

G

G

S

C

T

S

V

V

Y

A

I

I

A

I

G

G

A

S

G

G

P

P

V

V

G

G

L

L

A

A

A

L

S

T

A

A

R

Q

L

F

L

Y

G

S

A

P

A

A

V

K

V

L

I

I

I

M

G

V

D

D

V

L

N

D

T

D

V

N

R

R

L

L

A

E

H

T

A

A

K

L

A

S

Q

F

G

G

F

A

E

T

I

H

A

K

D

V

L

N

S

S

L

S

D

D

-

P

T

E

P

K

L

A

H

I

E

K

Q

E

I

I

A

Y

A

D

L

L

L

T

G

D

E

G

P

R

E

G

V

V

D

D

C

V

A

A

I

I

D

E

A

A

V

V

G

G

F

I

E

P

A

A

active site: C41 (= C47), T43 (≠ S49), H46 (= H52), H63 (= H68)
binding zinc ion: C41 (= C47), H63 (= H68), C93 (= C98), C96 (= C101), C99 (= C104), C107 (≠ V127)

P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
33% identity, 54% coverage: 37:250/399 of query aligns to 28:227/341 of P07913

query
sites
P07913

V

L

I

L

L

I

R

K

V

I

V

R

S

K

T

T

N

A

I

I

C
|
C

G

G

S

T

D

D

Q

V

H

H

M

I

V

Y

R

N

G

W

R

D

T

E

T

W

A

S

Q

Q

T

K

G

T

-

I

-

P

-

V

-

P

L

M

V

V

L

V

G

G

H

H

E

E

I

Y

T

V

G

G

E

E

V

V

I

V

E

G

K

I

G

G

S

Q

D

E

V

V

E

K

N

G

L

F

Q

K

I

I

G

G

D

D

L

R

V

V

S

S

V

G

P

E

F

G

N

H

V

I

A

T

C

C

G

G

R

H

C

C

R

R

S

N

C

C

K

R

E

G

M

G

H

R

T

T

G

H

V

L

C

C

-

R

-

N

-

T

L

I

S

G

V

V

N

G

P

V

A

N

R

R

P

P

G

G

G

C

A

F

Y

-

G

-

Y

-

V

-

D

-

M

-

G

-

D

-

W

-

T

-

G

-

Q

-

A

A

E

E

Y

Y

A

L

M

V

V

I

P

P

Y

A

A

-

D

-

F

F

N

N

L

A

L

F

K

K

L

I

P

P

D

D

R

N

D

I

R

S

A

-

M

-

E

-

K

-

I

-

R

D

D

D

L

L

T

A

C

A

L

I

S

F

D

D

I

P

L

F

P

G

T

N

G

A

Y

V

H

H

G

T

A

A

V

L

T

S

A

F

G

D

V

L

G

-

P

V

G

G

S

E

T

D

V

V

Y

L

I

V

A

S

G

G

A

A

G

G

P

P

V

I

G

G

L

I

A

M

A

A

S

V

A

A

R

K

L

H

L

V

G

G

A

A

A

R

V

N

V

V

I

V

I

I

G

T

D

D

V

V

N

N

T

E

V

Y

R

R

L

L

A

E

H

L

A

A

K

R

A

K

Q

M

G

G

F

I

E

T

I

R

A

A

D

V

L

N

S

V

L

A

D

K

T

E

P

N

L

L

H

N

E

D

Q

V

I

M

A

A

A

E

L

L

C38 (= C47) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).

3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
28% identity, 90% coverage: 37:394/399 of query aligns to 30:347/347 of 3gfbA

query
sites
3gfbA

V

V

I

L

L

I

R

K

V

V

V

L

S

A

T

T

N

S

I

I

C
|
C

G
|
G

S
x
T

D

D

Q

L

H
|
H

M

I

V

Y

R

E

G

W

R

N

T

E

T

W

A

A

Q

Q

T

S

G

R

L

I

-

K

-

P

-

Q

V

I

L

M

G

G

H
|
H

E
|
E

I

V

T

A

G

G

E

E

V

V

I

V

E

E

K

V

G

G

S

P

D

G

V

V

E

E

N

D

L

L

Q

Q

I

V

G

G

D

D

L

Y

V

I

S

S

V

V

P

E

F

T

N

H

V

I

A

V

C
|
C

G

G

R

K

C
|
C

R

Y

S

A

C
|
C

K

K

E

H

M

N

H

R

T

Y

G

H

V

V

C
|
C

L

Q

S

N

V

T

N
x
K

P

-

A

-

R

-

P

-

G

-

A

I

Y

F

G

G

Y

-

V

V

D

D

M

M

G

-

D

-

W

-

T

D

G

G

G

V

Q

F

A

A

E

H

Y

Y

A

A

M

I

V

V

P

P

Y

A

A

K

D

-

F

-

N

N

L

A

L

W

K

K

L

N

P

P

D

-

R

K

D

D

R

M

A

P

M

P

E

E

K

Y

I

A

R

A

D

L

L

Q

T

E

C
x
P

L

L

S

G

D

N

I
x
A

L

V

P

D

T

T

G

-

Y

-

H

-

G

-

A

-

V

V

T

L

A

A

G

G

V

P

G

I

P

A

G

G

S

R

T

S

V

T

Y

L

I

I

A

T

G
|
G

A
|
A

G
|
G

P
|
P

V
x
L

G

G

L

L

A

L

A

G

A

I

A

A

S

V

A

A

R

K

L

A

L

S

G

G

A

A

A

Y

V

P

V

V

I

I

I

V

G
x
S

D
x
E

V
x
P

N

S

T

E

V

F

R
|
R

L

R

A

K

H

L

A

A

K

K

A

K

Q

V

G

G

F

-

E

-

I

-

A

A

D

D

L

Y

S

V

L

V

D

N

T

-

P
|
P

L

F

H

E

E

E

Q

D

I

P

A

V

A

K

L

F

L

V

G

M

E

D

P

-

E

-

V

-

D

-

C

-

A

I

I

T

D

D

A

G

V

A

G

G

F

V

E

E

A

V

R

F

G

-

H

-

G

-

H

-

D

-

G

-

V

L

K

E

H
x
F

E
x
S

A

G

P
x
A

A

P

T

K

V
x
A

L

L

N

E

S

Q

L

G

M

L

G

K

V

A

V

V

R

T

V

P

A

G

G

G

K

R

I

V

G

S

I

L

P
x
L

G
|
G

L
|
L

Y

F

V

P

T

R

E

E

D

V

P

T

G

I

A

D

V

F

D

N

A

N

A

L

A

I

K
x
I

M
x
F

G

K

S

A

L

L

S

E

I

V

R

H

F

-

G

-

L

-

G

G

W
x
I

A
x
T

K

G

S

R

H

H

S

L

F

W

H

E

T

T

G

W

Q

Y

T

T

P

-

V

-

M

-

K

-

Y

-

N

V

R

S

A

S

L

L

M

I

Q

Q

A

S

I

-

M

-

W

-

D

G

R

K

I

L

N

N

I

L

A

D

E

P

V

I

G

T

V

H

Q

K

V

Y

I

K

S

G

L

F

D

D

Q

K

A

F

P

E

Q

E

G

A

Y

F

G

E

E

L

F

M

D

R

A

A

G

G

V

K

P

T

K

G

K
|
K

F

V

V

V

I

F

D

F

P

P

H

H

K

K

active site: C40 (= C47), G41 (= G48), T42 (≠ S49), H45 (= H52), H65 (= H68), E66 (= E69), C95 (= C98), C98 (= C101), C101 (= C104), C109 (= C112), K113 (≠ N116), P151 (≠ C167), A155 (≠ I171), K340 (= K387)
binding nicotinamide-adenine-dinucleotide: G173 (= G194), A174 (= A195), G175 (= G196), P176 (= P197), L177 (≠ V198), S196 (≠ G217), E197 (≠ D218), P198 (≠ V219), R202 (= R223), P217 (= P242), F241 (≠ H277), S242 (≠ E278), A244 (≠ P280), A247 (≠ V283), L264 (≠ P300), G265 (= G301), L266 (= L302), I280 (≠ K316), F281 (≠ M317), I289 (≠ W328), T290 (≠ A329)

Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
28% identity, 90% coverage: 37:394/399 of query aligns to 32:349/350 of Q5JI69

query
sites
Q5JI69

V

V

I

L

L

I

R

K

V

V

V

L

S

A

T

T

N

S

I

I

C

C

G

G

S

T

D

D

Q

L

H

H

M

I

V

Y

R

E

G

W

R

N

T

E

T

W

A

A

Q

Q

T

S

G

R

L

I

-

K

-

P

-

Q

V

I

L

M

G

G

H

H

E

E

I

V

T

A

G

G

E

E

V

V

I

V

E

E

K

V

G

G

S

P

D

G

V

V

E

E

N

D

L

L

Q

Q

I

V

G

G

D

D

L

Y

V

I

S

S

V

V

P

E

F

T

N

H

V

I

A

V

C

C

G

G

R

K

C

C

R

Y

S

A

C

C

K

K

E

H

M

N

H

R

T

Y

G

H

V

V

C

C

L

Q

S

N

V

T

N

K

P

-

A

-

R

-

P

-

G

-

A

I

Y

F

G

G

Y

-

V

V

D

D

M

M

G

-

D

-

W

-

T

D

G

G

G

V

Q

F

A

A

E

H

Y

Y

A

A

M

I

V

V

P

P

Y

A

A

K

D

-

F

-

N

N

L

A

L

W

K

K

L

N

P

P

D

-

R

K

D

D

R

M

A

P

M

P

E

E

K

Y

I

A

R

A

D

L

L

Q

T

E

C

P

L

L

S

G

D

N

I

A

L

V

P

D

T

T

G

-

Y

-

H

-

G

-

A

-

V

V

T

L

A

A

G

G

V

P

G

I

P

A

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Y

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A

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L179 (≠ V198) binding
E199 (≠ D218) binding
R204 (= R223) binding
LGL 266:268 (≠ PGL 300:302) binding
IT 291:292 (≠ WA 328:329) binding

O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
31% identity, 69% coverage: 37:311/399 of query aligns to 32:273/348 of O58389

query
sites
O58389

V

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