SitesBLAST
Comparing RR42_RS02235 FitnessBrowser__Cup4G11:RR42_RS02235 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
42% identity, 97% coverage: 3:303/311 of query aligns to 2:298/304 of 1wwkA
- active site: S96 (≠ N98), R230 (= R235), D254 (= D259), E259 (= E264), H278 (= H283)
- binding nicotinamide-adenine-dinucleotide: V100 (= V102), G146 (= G149), F147 (= F150), G148 (= G151), R149 (≠ D152), I150 (= I153), Y168 (≠ H171), D169 (= D172), P170 (= P173), V201 (= V206), P202 (= P207), T207 (= T212), T228 (= T233), S229 (≠ A234), D254 (= D259), H278 (= H283), G280 (≠ A285)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
41% identity, 98% coverage: 4:307/311 of query aligns to 5:302/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
41% identity, 98% coverage: 4:307/311 of query aligns to 4:301/526 of 3dc2A
Sites not aligning to the query:
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 9:294/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N98), A100 (≠ V102), R149 (≠ D152), I150 (= I153), Y168 (≠ H171), D169 (= D172), P170 (= P173), I171 (≠ A174), H200 (= H205), T201 (≠ V206), P202 (= P207), T207 (= T212), C228 (≠ T233), A229 (= A234), R230 (= R235), H277 (= H283), G279 (≠ A285)
7dkmA Phgdh covalently linked to oridonin (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 11:296/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V74), A102 (≠ V102), G148 (= G149), R151 (≠ D152), I152 (= I153), Y170 (≠ H171), D171 (= D172), P172 (= P173), I173 (≠ A174), H202 (= H205), T203 (≠ V206), P204 (= P207), T209 (= T212), C230 (≠ T233), A231 (= A234), R232 (= R235), H279 (= H283), G281 (≠ A285)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (= C21), K17 (≠ L24), I18 (≠ R25), E293 (≠ M297)
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 10:295/303 of 6plgA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 11:296/305 of 6plfA
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 10:295/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I148), G147 (= G149), L148 (≠ F150), G149 (= G151), R150 (≠ D152), I151 (= I153), G152 (= G154), D170 (= D172), H201 (= H205), T202 (≠ V206), P203 (= P207)
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 7:292/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G151), I148 (= I153), Y166 (≠ H171), D167 (= D172), P168 (= P173), I169 (≠ A174), I170 (≠ L175), H198 (= H205), T199 (≠ V206), L208 (= L215), R228 (= R235)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 10:295/302 of 6rihA
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 9:294/297 of 6rj3A
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 10:295/301 of 6rj5A
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
37% identity, 91% coverage: 18:300/311 of query aligns to 15:300/533 of O43175
- T78 (≠ V74) binding
- R135 (= R131) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ DI 152:153) binding
- D175 (= D172) binding
- T207 (≠ V206) binding
- CAR 234:236 (≠ TAR 233:235) binding
- D260 (= D259) binding
- V261 (= V260) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIAG 283:286) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 91% coverage: 18:300/311 of query aligns to 9:286/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (≠ D152), Y160 (≠ H171), D161 (= D172), P162 (= P173), I164 (≠ L175), L179 (= L192), T193 (≠ V206), P194 (= P207), S198 (≠ A211), L202 (= L215)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
37% identity, 89% coverage: 34:311/311 of query aligns to 36:313/334 of 5aovA
- active site: L100 (≠ N98), R241 (= R235), D265 (= D259), E270 (= E264), H288 (= H283)
- binding glyoxylic acid: M52 (≠ R50), L53 (≠ N51), L53 (≠ N51), Y74 (≠ L72), A75 (≠ G73), V76 (= V74), G77 (= G75), R241 (= R235), H288 (= H283)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V74), T104 (≠ V102), F158 (= F150), G159 (= G151), R160 (≠ D152), I161 (= I153), S180 (≠ D172), R181 (≠ P173), A211 (≠ H205), V212 (= V206), P213 (= P207), T218 (= T212), I239 (≠ T233), A240 (= A234), R241 (= R235), H288 (= H283), G290 (≠ A285)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
39% identity, 87% coverage: 34:305/311 of query aligns to 35:312/332 of 6biiA
- active site: L99 (≠ N98), R240 (= R235), D264 (= D259), E269 (= E264), H287 (= H283)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V74), T103 (≠ V102), G156 (= G149), F157 (= F150), G158 (= G151), R159 (≠ D152), I160 (= I153), A179 (= A174), R180 (≠ L175), S181 (≠ P176), K183 (≠ D178), V211 (= V206), P212 (= P207), E216 (≠ A211), T217 (= T212), V238 (≠ T233), A239 (= A234), R240 (= R235), D264 (= D259), H287 (= H283), G289 (≠ A285)
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
35% identity, 87% coverage: 34:303/311 of query aligns to 88:364/466 of P87228
- S258 (≠ V209) modified: Phosphoserine
Sites not aligning to the query:
- 87 modified: Phosphoserine
8atiA Human ctbp2(31-364) in complex with rai2 peptide(315-322)
40% identity, 80% coverage: 58:307/311 of query aligns to 58:313/330 of 8atiA
- binding nicotinamide-adenine-dinucleotide: S74 (≠ V74), T102 (≠ V102), G155 (= G149), G157 (= G151), R158 (≠ D152), T159 (≠ I153), D178 (= D172), P179 (= P173), Y180 (≠ A174), H210 (= H205), C211 (≠ V206), N212 (≠ P207), A238 (≠ T233), R240 (= R235), H289 (= H283), A291 (= A285), W292 (≠ G286)
Sites not aligning to the query:
4lcjA Ctbp2 in complex with substrate mtob (see paper)
40% identity, 80% coverage: 58:307/311 of query aligns to 58:313/330 of 4lcjA
- active site: A98 (≠ N98), R240 (= R235), D264 (= D259), E269 (= E264), H289 (= H283)
- binding 4-(methylsulfanyl)-2-oxobutanoic acid: I72 (≠ L72), G73 (= G73), S74 (≠ V74), G75 (= G75), R240 (= R235), H289 (= H283), W292 (≠ G286)
- binding nicotinamide-adenine-dinucleotide: S74 (≠ V74), T102 (≠ V102), I154 (= I148), G155 (= G149), G157 (= G151), R158 (≠ D152), T159 (≠ I153), D178 (= D172), Y180 (≠ A174), H210 (= H205), C211 (≠ V206), N212 (≠ P207), N214 (≠ V209), N217 (≠ T212), A238 (≠ T233), A239 (= A234), R240 (= R235), H289 (= H283), W292 (≠ G286)
Sites not aligning to the query:
6v89A Human ctbp1 (28-375) in complex with amp (see paper)
39% identity, 80% coverage: 58:307/311 of query aligns to 59:314/332 of 6v89A
Query Sequence
>RR42_RS02235 FitnessBrowser__Cup4G11:RR42_RS02235
MKQVCISEFMDADAVAVLDACVRLRYEPGWVDQRDALLGALAQTDALIVRNRTQVDTELL
DAAPALRVVGRLGVGLDNIDVKSCEARGIRVIPATGANARSVAEYVVTTALMLLRGAYQS
DAAMVAGKWPRARLSEGREALGKTLGLIGFGDIGRLTARLAQALGMQVVAHDPALPGDHP
VWRDTGVEPMKLEALLASADAVSLHVPLVPATRHLINAERLRGMKRGAVLVNTARGGVVD
ETALAAALHEGLLAGAALDVFEAEPLGAGGILAHAPNLVLTPHIAGVSFEANVRVSMMIA
DAVKNALGVQR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory