SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS05085 FitnessBrowser__Cup4G11:RR42_RS05085 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

P52759 2-iminobutanoate/2-iminopropanoate deaminase; Liver perchloric acid-soluble protein; L-PSP; Reactive intermediate imine deaminase A homolog; Translation inhibitor L-PSP ribonuclease; UK114 antigen homolog; rp14.5; EC 3.5.99.10 from Rattus norvegicus (Rat) (see paper)
39% identity, 57% coverage: 57:136/140 of query aligns to 51:129/137 of P52759

query
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P52759

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

7cd4A Crystal structure of the s103f mutant of bacillus subtilis (natto) yabj protein. (see paper)
41% identity, 56% coverage: 56:134/140 of query aligns to 44:121/124 of 7cd4A

query
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7cd4A

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binding zinc ion: E47 (= E59)

Sites not aligning to the query:

binding zinc ion: 5, 8

P80601 2-iminobutanoate/2-iminopropanoate deaminase; 14.3 kDa perchloric acid soluble protein; Translation inhibitor L-PSP ribonuclease; UK114 antigen; EC 3.5.99.10; EC 3.1.-.- from Capra hircus (Goat) (see paper)
38% identity, 57% coverage: 57:136/140 of query aligns to 51:129/137 of P80601

query
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P80601

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

3vczB 1.80 angstrom resolution crystal structure of a putative translation initiation inhibitor from vibrio vulnificus cmcp6
37% identity, 63% coverage: 49:136/140 of query aligns to 37:127/127 of 3vczB

query
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3vczB

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binding magnesium ion: D79 (≠ N91), N81 (= N93)

2b33B Crystal structure of a putative endoribonuclease (tm0215) from thermotoga maritima msb8 at 2.30 a resolution
43% identity, 57% coverage: 57:136/140 of query aligns to 46:124/126 of 2b33B

query
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2b33B

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Sites not aligning to the query:

binding calcium ion: 35, 37

Q94JQ4 Reactive Intermediate Deaminase A, chloroplastic; 2-iminobutanoate/2-iminopropanoate deaminase; EC 3.5.99.10 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
30% identity, 85% coverage: 16:134/140 of query aligns to 77:185/187 of Q94JQ4

query
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Q94JQ4

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R165 (= R113) mutation to A: Loss of activity.

2uynA Crystal structure of e. Coli tdcf with bound 2-ketobutyrate (see paper)
35% identity, 56% coverage: 56:134/140 of query aligns to 44:124/127 of 2uynA

query
sites
2uynA

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binding 2-ketobutyric acid: F83 (= F95), R104 (= R113), S105 (≠ T114), C106 (≠ T115)

2uykC Crystal structure of e. Coli tdcf with bound serine (see paper)
35% identity, 56% coverage: 56:134/140 of query aligns to 44:124/127 of 2uykC

query
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2uykC

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binding serine: R104 (= R113), S105 (≠ T114), C106 (≠ T115)

5hp8A Crystal structures of rida in complex with pyruvate (see paper)
38% identity, 56% coverage: 57:134/140 of query aligns to 30:106/108 of 5hp8A

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5hp8A

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binding pyruvic acid: R86 (= R113), T88 (= T115)

A0A1J1DL12 2-iminobutanoate/2-iminopropanoate deaminase; Allergen Der f 34; Enamine/imine deaminase; Allergen Der f 34.0101; EC 3.5.99.10 from Dermatophagoides farinae (American house dust mite) (see paper)
31% identity, 55% coverage: 57:133/140 of query aligns to 49:124/128 of A0A1J1DL12

query
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A0A1J1DL12

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N59 (= N67) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-91; A-105; A-114 and A-120.
N91 (= N99) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-105; A-114 and A-120.
R105 (= R113) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-114 and A-120.
P114 (= P122) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-120.
E120 (= E129) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-114.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
19 Y→A: Loss of imine deaminase activity; when associated with A-33; A-59; A-91; A-105; A-114 and A-120.
33 G→A: Loss of imine deaminase activity; when associated with A-19; A-59; A-91; A-105; A-114 and A-120.

Q7CP78 2-iminobutanoate/2-iminopropanoate deaminase; Enamine/imine deaminase; EC 3.5.99.10 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
33% identity, 61% coverage: 51:136/140 of query aligns to 40:127/128 of Q7CP78

query
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Q7CP78

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R105 (= R113) mutation to A: Deaminase activity is defective. At pH 8 and 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
E120 (= E129) Important for catalytic activity at high pH; mutation to A: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.; mutation to K: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.

Sites not aligning to the query:

17 Y→F: Deaminase activity is defective. At pH 8, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.

5v4dA Crystal structure of the protein of unknown function of the conserved rid protein family yyfa from yersinia pestis
38% identity, 56% coverage: 56:134/140 of query aligns to 47:125/127 of 5v4dA

query
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5v4dA

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E

E

I

I

K

E

A

V

V

I

A

A

binding calcium ion: D91 (≠ E100), D95 (≠ E104)

P0AFQ5 3-aminoacrylate deaminase RutC; 3-AA deaminase; EC 3.5.-.- from Escherichia coli (strain K12) (see paper)
27% identity, 90% coverage: 10:135/140 of query aligns to 10:125/128 of P0AFQ5

query
sites
P0AFQ5

G

G

K

S

A

S

T

A

P

P

R

L

G

A

R

P

F

F

P

V

H

P

V

G

K

T

R

L

A

A

G

D

D

G

F

V

L

V

F

Y

V

V

S

S

G

G

T

T

S

L

S

A

R

F

R

D

A

Q

D

H

N

N

S

N

F

V

A

L

G

F

A

A

S

D

A

-

D

-

A

-

M

-

G

-

V

-

T

-

A

-

L

-

D

D

I

P

H

K

E

A

Q

Q

T

T

R

R

A

H

V

V

I

L

G

E

N

T

I

I

R

R

E

K

I

V

L

I

Q

E

S

T

V

A

G

G

C

G

D

T

L

M

S

A

D

D

L

V

V

T

E

F

I

N

T

S

T

I

F

F

L

I

V

T

N

D

M

W

N

K

D

N

F

Y

G

A

Y

I

N

N

E

E

V

I

Y

Y

G

A

E

E

Y

F

F

F

D

P

Y

G

D

D

G

K

P

P

T

A

R
|
R

T

F

T

C

V

I

A

Q

V

C

H

-

Q

G

L

L

P

V

H

K

P

P

H

D

L

A

L

L

I

V

E

E

I

I

K

A

A

T

V

I

A

A

Y

H

R104 (= R113) mutation to A: Loss of activity.

3k0tC Crystal structure of pspto -psp protein in complex with d-beta-glucose from pseudomonas syringae pv. Tomato str. Dc3000 (see paper)
31% identity, 48% coverage: 57:123/140 of query aligns to 45:111/124 of 3k0tC

query
sites
3k0tC

I

I

H

E

E

A

Q

Q

T

I

R

T

A

Q

V

V

I

F

G

E

N

N

I

L

R

K

E

S

I

V

L

A

Q

Q

S

A

V

A

G

G

C

G

D

S

L

F

S

K

D

D

L

I

V

V

E

K

I

L

T

N

T

I

F

F

L

L

V

T

N

D

M

L

N

G

D

H

F
|
F

G

A

G

K

Y

V

N

N

E

E

V

I

Y

M

G

G

E

S

Y

Y

F

F

D

S

Y

Q

D

P

G

Y

P

P

T

A

R
|
R

T

A

V

I

A

G

V

V

H

A

Q

A

L

L

P

P

H
x
R

binding beta-D-glucopyranose: F83 (= F95), R101 (= R113), R111 (≠ H123)

Sites not aligning to the query:

binding beta-D-glucopyranose: 16, 30, 32, 116

Query Sequence

>RR42_RS05085 FitnessBrowser__Cup4G11:RR42_RS05085
MTTLSKVLTGKATPRGRFPHVKRAGDFLFVSGTSSRRADNSFAGASADAMGVTALDIHEQ
TRAVIGNIREILQSVGCDLSDLVEITTFLVNMNDFGGYNEVYGEYFDYDGPTRTTVAVHQ
LPHPHLLIEIKAVAYQPLPR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory