SitesBLAST
Comparing RR42_RS11100 FitnessBrowser__Cup4G11:RR42_RS11100 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
66% identity, 95% coverage: 9:492/509 of query aligns to 4:489/489 of P25737
- Y102 (= Y107) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- W106 (≠ L111) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- K163 (= K168) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- F216 (= F221) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- E222 (= E227) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
- E230 (= E235) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
- D275 (= D280) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
- D278 (= D283) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.
- E438 (≠ Q443) mutation to A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
- D443 (≠ V448) mutation to A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
- D446 (≠ R451) mutation to A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
37% identity, 92% coverage: 13:481/509 of query aligns to 79:553/590 of P04817
- P113 (≠ S47) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
- P148 (= P82) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
- V149 (= V83) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
- S152 (= S86) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
- Y173 (= Y107) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
- G308 (= G234) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
- P313 (= P239) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
- TS 354:355 (≠ VT 281:282) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
- Y356 (≠ D283) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
- W451 (= W378) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
- F461 (= F388) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.
P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
37% identity, 91% coverage: 14:477/509 of query aligns to 4:453/469 of P46349
- G33 (≠ A43) mutation to D: Lack of activity.
- G42 (= G52) mutation to S: Lack of activity.
- G301 (≠ S320) mutation to V: Lack of activity.
- G338 (≠ T357) mutation to E: Lack of activity.
- F341 (≠ V360) mutation to S: Lack of activity.
- G414 (≠ V434) mutation to R: Lack of activity.
Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
31% identity, 95% coverage: 18:502/509 of query aligns to 80:575/587 of Q9URZ4
Sites not aligning to the query:
- 29 modified: Phosphoserine
- 30 modified: Phosphoserine
- 37 modified: Phosphoserine
P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
34% identity, 94% coverage: 12:492/509 of query aligns to 80:569/602 of P19145
- A297 (≠ Q224) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.
Sites not aligning to the query:
- 76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
35% identity, 86% coverage: 1:438/509 of query aligns to 7:426/458 of P24207
- R26 (= R25) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
- P54 (= P53) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
- F87 (= F87) mutation to L: No effect on phenylalanine transport activity.
- F90 (≠ Y90) mutation to L: 65% of wild-type phenylalanine transport activity.
- Y92 (≠ A92) mutation to L: 41% of wild-type phenylalanine transport activity.
- Y94 (= Y94) mutation to L: 69% of wild-type phenylalanine transport activity.
- W95 (≠ V95) mutation to L: 10% of wild-type phenylalanine transport activity.
- F98 (≠ G98) mutation to L: No effect on phenylalanine transport activity.
- F101 (= F101) mutation to L: 38% of wild-type phenylalanine transport activity.
- W105 (= W105) mutation to L: 39% of wild-type phenylalanine transport activity.
- Y107 (= Y107) mutation to L: No effect on phenylalanine transport activity.
- W108 (= W108) mutation to L: 71% of wild-type phenylalanine transport activity.
- F111 (≠ L111) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
- E118 (= E118) mutation E->G,L,V,N: Loss of activity.
- K168 (= K168) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
- E226 (= E227) mutation E->A,Q,K,R,W: Loss of activity.
- R252 (= R253) mutation R->D,E,F,W,P: Loss of activity.
- P341 (= P350) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.
Sites not aligning to the query:
- 442 P→A: 46% of wild-type phenylalanine transport activity.; P→G: 52% of wild-type phenylalanine transport activity.; P→L: 43% of wild-type phenylalanine transport activity.
P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
36% identity, 81% coverage: 16:425/509 of query aligns to 9:405/457 of P15993
- Y103 (≠ L111) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.
P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
29% identity, 92% coverage: 9:477/509 of query aligns to 136:613/663 of P48813
Sites not aligning to the query:
- 132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 92% coverage: 18:486/509 of query aligns to 277:818/852 of Q03770
- T382 (≠ Q123) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.
P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
24% identity, 67% coverage: 73:411/509 of query aligns to 72:396/461 of P76037
- Y110 (≠ L111) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.
6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
27% identity, 83% coverage: 17:438/509 of query aligns to 22:426/458 of 6f34A
- binding arginine: I40 (≠ A35), G42 (= G37), T43 (= T38), G44 (= G39), E115 (= E96), Y116 (≠ E97), A119 (≠ G100), F228 (= F221), A229 (≠ S222), I231 (≠ Q224), V314 (≠ A316)
- binding cholesterol: W201 (≠ M181), Y202 (≠ M182)
- binding : G28 (≠ K23), F30 (≠ R25), D31 (≠ H26), M34 (= M29), A178 (≠ G158), R179 (≠ E159), A186 (≠ L166), I187 (= I167), A190 (= A170), L194 (≠ I174), Q296 (≠ A296), V299 (≠ A301)
5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
27% identity, 83% coverage: 17:438/509 of query aligns to 20:424/456 of 5oqtA
- binding alanine: I38 (≠ A35), G40 (= G37), T41 (= T38), G42 (= G39), F226 (= F221), A227 (≠ S222), I229 (≠ Q224)
- binding : E24 (≠ K21), G26 (≠ K23), F28 (≠ R25), D29 (≠ H26), M32 (= M29), A176 (≠ G158), R177 (≠ E159), A184 (≠ L166), A188 (= A170), L192 (≠ I174), Q294 (≠ A296), V297 (≠ A301)
6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
21% identity, 86% coverage: 30:465/509 of query aligns to 12:424/433 of 6f2wA
P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
25% identity, 69% coverage: 14:363/509 of query aligns to 24:398/629 of P30825
- N226 (≠ P191) modified: carbohydrate, N-linked (GlcNAc...) asparagine
Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
24% identity, 77% coverage: 2:393/509 of query aligns to 13:391/531 of Q9QXW9
- Y130 (≠ I115) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
- N133 (≠ E118) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
- F242 (= F221) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.
Q22397 Amino acid transporter protein 6 from Caenorhabditis elegans (see paper)
26% identity, 42% coverage: 143:354/509 of query aligns to 152:349/523 of Q22397
Sites not aligning to the query:
- 521:523 PDZ-binding motif; mutation Missing: Abolishes the interaction with nrfl-1.
Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
23% identity, 69% coverage: 18:366/509 of query aligns to 36:379/535 of Q9UHI5
- I53 (≠ A35) binding
- Y93 (≠ L74) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
- N134 (≠ E118) Important for substrate specificity; binding ; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
- C154 (vs. gap) modified: Interchain (with C-210 in SLC3A2)
- W174 (vs. gap) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
- F243 (= F221) mutation to A: Abolishes leucine and tryptophan transport activities.
- G246 (≠ Q224) Important for substrate specificity; binding ; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
- V302 (≠ T282) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity
Sites not aligning to the query:
- 395 binding ; N→Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
- 396 Y→A: Strongly reduces L-leucine uptake activity.
- 402 T → M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity
- 418 R → C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity
- 460 V → E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization
7b00A Human lat2-4f2hc complex in the apo-state (see paper)
24% identity, 67% coverage: 30:368/509 of query aligns to 8:341/457 of 7b00A
Sites not aligning to the query:
7cmiB The lat2-4f2hc complex in complex with leucine (see paper)
24% identity, 67% coverage: 30:368/509 of query aligns to 8:341/458 of 7cmiB
7cmhB The lat2-4f2hc complex in complex with tryptophan (see paper)
24% identity, 67% coverage: 30:368/509 of query aligns to 8:341/458 of 7cmhB
Sites not aligning to the query:
Query Sequence
>RR42_RS11100 FitnessBrowser__Cup4G11:RR42_RS11100
MSSHPHPHQHPVVEHDDLQRKLKARHLTMIAIGGAVGTGLFVASGASISQAGPGGALLMY
CLIGLMVYCLMTSLGELAVHMPVAGSFVTYSALYVEEGFGFALGWSYWFSLAVTIAVELA
AAQLVMQYWFPHVSGVVWSAGFLLLMFGLNAFSVRGFGEAEYWFALIKVATILIFLAAGL
MMIFGIMQGGPQSGWHNFTLGDAPFVGGIPAMFGVAMIAGFSFQGTETVGVAAGEAADPA
RTIPRAIRQTFWRILLFYVLAILIIGVLIPYTDPSLLRNDVTDIGVSPFALVFRHAGLAF
AAGLMNAVVLTALLSAGTSSMYASTRILYGLAVSGRAPRALARLSANGVPYVALFATTAV
GALCFLSSLFGDKAVYLWLLNTSGMTGFIAWLGIAISHYRFRRGLVHQGYKPSDLAYRSP
LYPFGPLFAIVLCVVIVLGQNYQAFSDVRGRWLEIVGTYIGVPLFLVLWLGYRLVKKTRL
IDYEDMPFNCKRAAEAGPAAASLPRHSAG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory