SitesBLAST

Q7CRQ0 Uronate dehydrogenase; D-galacturonate dehydrogenase; D-glucuronate dehydrogenase; Hexuronate dehydrogenase; EC 1.1.1.203 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
45% identity, 94% coverage: 1:255/272 of query aligns to 1:256/265 of Q7CRQ0

query
sites
Q7CRQ0

M

M

T

K

K

R

I

L

A

L

L

V

S

T

G

G

A

A

G

A

G

G

Q

Q

L

L

G

G

T

R

V

V

L

M

R

R

T

E

A

R

L

L

L

A

A

P

R

M

G

A

Y

E

D

I

L

L

R

R

S

L

S

A

G

D

N

L

S

S

Q

-

S

P

L

L

A

D

P

P

V

A

A

G

E

P

G

N

E

E

V

C

M

V

R

Q

G

C

D

D

L

L

C

A

D

D

P

A

A

N

V

A

V

V

D

N

R

A

L

M

L

V

D

A

G

G

V

C

D

D

V

G

L

I

I

V

H

H

M

L

A

G

G

G

T

I

S

S

V

V

E

E

R

K

P

P

L

F

P

E

E

Q

I

I

I

L

D

Q

N

G

N

N

L

I

R

I

A

G

L

L

V

Y

E

N

V

L

Y

Y

E

E

G

A

A

A

R

R

R

A

Q

H

K

G

V

Q

G

P

R

R

I

I

V

V

F

F

A

A

S

S

N

N

H

H

A

T

I

I

G

G

M

Y

Y

Y

P

P

V

Q

D

T

Q

E

Q

R

L

L

S

G

T

P

D

D

C

V

E

P

F

A

R

R

P

P

D

D

G

G

F

L

Y
|
Y

G

G

L

V

S

S

K

K

M

C

W

F

G

G

E

E

G

N

L

L

A

A

R

R

L

M

Y

Y

W

F

D

D

K

K

H

F

G

G

I

Q

E

E

S

T

V

A

C

L

V

V

R

R

I

I

G

G

S

S

C

C

V

T

E

P

R

E

P

P

Q

N

E

N

F

Y

R

R

H

M

L

L

S

S

T

T

W

W

L

F

G

S

H

H

E

D

D

D

L

F

V

V

H

S

L

L

I

I

E

E

Q

A

G

V

I

F

T

R

V

A

P

P

D

V

L

L

G

G

F

C

L

P

V

V

W

W

G

G

V

A

S

S

A

A

N

N

A

D

R

A

S

G

C

W

W

W

N

D

N

N

D

S

G

H

A

L

A

G

R

F

L

L

R

G

Y

W

R

K

P

P

K

K

Q

D

N

N

A

A

E

E

D

A

F

F

-

R

-

H

A

I

E

T

E

E

I

T

L

T

A

P

G

P

P

P

N

D

P

P

L

N

D

D

A

A

I

L

G

V

R

R

Q

-

Y

F

Q

Q

G

G

S

T

F

F

Y134 (= Y135) mutation to A: 0.1% of wild-type activity.

3rfxA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens, y136a mutant complexed with NAD (see paper)
45% identity, 94% coverage: 1:255/272 of query aligns to 2:257/265 of 3rfxA

query
sites
3rfxA

M

M

T

K

K

R

I

L

A

L

L

V

S

T

G
|
G

A

A

G

A

G
|
G

Q
|
Q

L
|
L

G

G

T

R

V

V

L

M

R

R

T

E

A

R

L

L

L

A

A

P

R

M

G

A

Y

E

D

I

L

L

R

R

S

L

S

A

G
x
D

N
x
L

S

S

Q

-

S

P

L

L

A

D

P

P

V

A

A

G

E

P

G

N

E

E

V

C

M

V

R

Q

G

C

D
|
D

L
|
L

C

A

D

D

P

A

A

N

V

A

V

V

D

N

R

A

L

M

L

V

D

A

G

G

V

C

D

D

V

G

L

I

I

V

H

H

M

L

A

G

G
|
G

T

I

S
|
S

V

V

E

E

R

K

P

P

L

F

P

E

E

Q

I

I

I

L

D

Q

N

G

N

N

L

I

R

I

A

G

L

L

V

Y

E

N

V

L

Y

Y

E

E

G

A

A

A

R

R

R

A

Q

H

K

G

V

Q

G

P

R

R

I

I

V

V

F

F

A

A

S

S

N

N

H

H

A

T

I

I

G

G

M

Y

Y

Y

P

P

V

Q

D

T

Q

E

Q

R

L

L

S

G

T

P

D

D

C

V

E

P

F

A

R

R

P

P

D

D

G

G

F

L

Y

A

G

G

L

V

S

S

K

K

M

C

W

F

G

G

E

E

G

N

L

L

A

A

R

R

L

M

Y

Y

W

F

D

D

K

K

H

F

G

G

I

Q

E

E

S

T

V

A

C

L

V

V

R

R

I

I

G

G

S

S

C

C

V

T

E

P

R

E

P

P

Q

N

E

N

F

Y

R

R

H

M

L

L

S

S

T

T

W

W

L

F

G

S

H

H

E

D

D

D

L

F

V

V

H

S

L

L

I

I

E

E

Q

A

G

V

I

F

T

R

V

A

P

P

D

V

L

L

G

G

F

C

L

P

V

V

W

W

G

G

V

A

S

S

A

A

N

N

A

D

R

A

S

G

C

W

W

W

N

D

N

N

D

S

G

H

A

L

A

G

R

F

L

L

R

G

Y

W

R

K

P

P

K

K

Q

D

N

N

A

A

E

E

D

A

F

F

-

R

-

H

A

I

E

T

E

E

I

T

L

T

A

P

G

P

P

P

N

D

P

P

L

N

D

D

A

A

I

L

G

V

R

R

Q

-

Y

F

Q

Q

G

G

S

T

F

F

binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), Q13 (= Q12), L14 (= L13), D33 (≠ G32), L34 (≠ N33), D50 (= D50), L51 (= L51), G72 (= G72), S74 (= S74)

6k0iA Crystal structure of udp-glucose 4-epimerase from bifidobacterium longum in complex with NAD+ and udp-glc (see paper)
30% identity, 54% coverage: 2:147/272 of query aligns to 1:161/335 of 6k0iA

query
sites
6k0iA

T

T

K

T

I

V

A

L

L

V

S

T

G
|
G

A

G

G

A

G
|
G

Q
x
F

L
x
I

G

A

T

T

V

H

L

T

R

D

T

I

A

E

L

L

A

N

R

K

G

G

Y

Y

D

D

L

V

R

I

S

S

S

V

G
x
D

N
|
N

S

Y

Q

G

S
x
N

L
x
S

A

S

P

P

V

V

A

A

-

L

-

E

-

R

-

V

-

E

-

Q

-

I

E

T

G

G

E

K

E

P

V

V

M

K

R

R

-

Y

-

D

G

G

D
|
D

L
x
V

C

R

D

D

P

E

A

A

V

L

V

M

D

E

R

R

L

V

L

F

-

A

-

E

D

N

G

N

V

I

D

D

V

W

L

V

I

I

H

H

M
x
F

A
|
A

-
x
G

-

L

-
x
K

-

A

-

V

G

G

T

E

S

S

V

V

E

A

R

K

P

P

L

I

P

-

E

E

I

Y

D

D

N
|
N

N

N

L

L

R

Y

A

S

L

T

V

L

E

V

V

L

Y

L

E

K

G

V

A

M

R

K

Q

H

K

N

V

V

G

K

R

K

I

I

V

I

F

F

A
x
S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

T

-

P

-

K

-

E

Y

L

P

P

V

I

D

T

Q

E

L

T

S

P

T

T

D

G

C

G

E

T

F

T

R

N

P

P

D

-

G

-

F

-

Y
|
Y

G

G

L

T

S

S

K
|
K

M

L

W

F

G

Q

E

E

G

Q

L

I

A

L

R

R

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (≠ Q12), I12 (≠ L13), D31 (≠ G32), N32 (= N33), N35 (≠ S36), S36 (≠ L37), D58 (= D50), V59 (≠ L51), F80 (≠ M70), A81 (= A71), G82 (vs. gap), K84 (vs. gap), N99 (= N85), S122 (≠ A108), S123 (= S109), Y149 (= Y135), K153 (= K139)
binding uridine-5'-diphosphate-glucose: K84 (vs. gap), S124 (= S110), Y149 (= Y135)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-5'-diphosphate-glucose: 178, 179, 198, 199, 200, 216, 217, 218, 231, 233, 268, 291, 294

6k0hA Crystal structure of udp-glucose 4-epimerase from bifidobacterium longum in complex with NAD+ and udp-glcnac (see paper)
30% identity, 54% coverage: 2:147/272 of query aligns to 1:161/335 of 6k0hA

query
sites
6k0hA

T

T

K

T

I

V

A

L

L

V

S

T

G
|
G

A

G

G

A

G
|
G

Q
x
F

L
x
I

G

A

T

T

V

H

L

T

R

D

T

I

A

E

L

L

A

N

R

K

G

G

Y

Y

D

D

L

V

R

I

S

S

S

V

G
x
D

N
|
N

S

Y

Q
x
G

S
x
N

L
x
S

A

S

P

P

V

V

A

A

-

L

-

E

-

R

-

V

-

E

-

Q

-

I

E

T

G

G

E

K

E

P

V

V

M

K

R

R

-

Y

-

D

G

G

D
|
D

L
x
V

C

R

D

D

P

E

A

A

V

L

V

M

D

E

R

R

L

V

L

F

-

A

-

E

D

N

G

N

V

I

D

D

V

W

L

V

I

I

H

H

M
x
F

A
|
A

-
x
G

-

L

-
x
K

-

A

-

V

G

G

T

E

S

S

V

V

E

A

R

K

P

P

L

I

P

-

E

E

I

Y

D

D

N
|
N

N

N

L

L

R

Y

A

S

L

T

V

L

E

V

V

L

Y

L

E

K

G

V

A

M

R

K

Q

H

K

N

V

V

G

K

R

K

I

I

V

I

F

F

A

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

T

-

P

-

K

-

E

Y

L

P

P

V

I

D

T

Q

E

L

T

S

P

T

T

D

G

C

G

E

T

F

T

R

N

P

P

D

-

G

-

F

-

Y
|
Y

G

G

L

T

S

S

K
|
K

M

L

W

F

G

Q

E

E

G

Q

L

I

A

L

R

R

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (≠ Q12), I12 (≠ L13), D31 (≠ G32), N32 (= N33), G34 (≠ Q35), N35 (≠ S36), S36 (≠ L37), D58 (= D50), V59 (≠ L51), F80 (≠ M70), A81 (= A71), G82 (vs. gap), K84 (vs. gap), N99 (= N85), S123 (= S109), Y149 (= Y135), K153 (= K139)
binding uridine-diphosphate-n-acetylglucosamine: K84 (vs. gap), S124 (= S110), Y149 (= Y135)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-diphosphate-n-acetylglucosamine: 178, 179, 198, 199, 200, 216, 217, 218, 231, 233, 268, 291, 294

6k0gA Crystal structure of udp-glucose 4-epimerase from bifidobacterium longum in complex with NAD+ and udp (see paper)
30% identity, 54% coverage: 2:147/272 of query aligns to 1:161/335 of 6k0gA

query
sites
6k0gA

T

T

K

T

I

V

A

L

L

V

S

T

G
|
G

A

G

G

A

G
|
G

Q
x
F

L
x
I

G

A

T

T

V

H

L

T

R

D

T

I

A

E

L

L

A

N

R

K

G

G

Y

Y

D

D

L

V

R

I

S

S

S

V

G
x
D

N
|
N

S

Y

Q

G

S
x
N

L
x
S

A

S

P

P

V

V

A

A

-

L

-

E

-

R

-

V

-

E

-

Q

-

I

E

T

G

G

E

K

E

P

V

V

M

K

R

R

-

Y

-

D

G

G

D
|
D

L
x
V

C

R

D

D

P

E

A

A

V

L

V

M

D
x
E

R

R

L

V

L

F

-

A

-

E

D

N

G

N

V

I

D

D

V

W

L

V

I

I

H

H

M
x
F

A
|
A

-
x
G

-

L

-
x
K

-

A

-

V

G

G

T

E

S

S

V

V

E

A

R

K

P

P

L

I

P

-

E

E

I

Y

D

D

N
|
N

N

N

L

L

R

Y

A

S

L

T

V

L

E

V

V

L

Y

L

E

K

G

V

A

M

R

K

Q
x
H

K

N

V

V

G

K

R

K

I

I

V

I

F

F

A
x
S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

T

-

P

-

K

-

E

Y

L

P

P

V

I

D

T

Q

E

L

T

S

P

T

T

D

G

C

G

E

T

F

T

R

N

P

P

D

-

G

-

F

-

Y
|
Y

G

G

L

T

S

S

K
|
K

M

L

W

F

G

Q

E

E

G

Q

L

I

A

L

R

R

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding magnesium ion: E66 (≠ D58), H114 (≠ Q100)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (≠ Q12), I12 (≠ L13), D31 (≠ G32), N32 (= N33), N35 (≠ S36), S36 (≠ L37), D58 (= D50), V59 (≠ L51), F80 (≠ M70), A81 (= A71), G82 (vs. gap), K84 (vs. gap), N99 (= N85), S122 (≠ A108), S123 (= S109), Y149 (= Y135), K153 (= K139)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-5'-diphosphate: 179, 198, 199, 200, 216, 217, 218, 231, 233, 268, 291, 294

1r6dA Crystal structure of desiv double mutant (dtdp-glucose 4,6- dehydratase) from streptomyces venezuelae with NAD and dau bound (see paper)
27% identity, 80% coverage: 3:220/272 of query aligns to 2:225/322 of 1r6dA

query
sites
1r6dA

K

R

I

L

A

L

L

V

S

T

G

G

A

G

G

A

G
|
G

Q
x
F

L
x
I

G

G

T

S

V

H

L

F

R

V

T

R

A

Q

L

L

A

A

R

G

G

A

Y

Y

-

P

-

D

-

V

-

P

-

A

-

D

-

E

-

V

-

I

-

V

-

L

-
x
D

D
x
S

L
|
L

R
x
T

S

Y

S
x
A

G
|
G

N

N

S

R

Q

A

S

N

L

L

A

A

P

P

V

V

A

D

E

A

G

D

E

P

E

R

V

L

-

R

-

F

M

V

R

H

G

G

D
|
D

L
x
I

C

R

D

D

P

A

A

G

V

L

D

A

R

R

L

E

L

L

D

R

G

G

V

V

D

D

V

A

L

I

I

V

H

H

M
x
F

A
|
A

G
x
A

T

E

S
|
S

-
x
H

V

V

E

D

R

R

P

S

L

I

-

A

-

G

-

A

P

S

E

V

I

F

I

T

D

E

N
x
T

N

N

L

V

R

Q

A

G

L

T

V

Q

E

T

V

L

Y

L

E

Q

G

C

A

A

R

V

R

D

Q

A

K

G

V

V

G

G

R

R

I

V

V

V

F

H

A
x
V

S
|
S

S
x
T

N
|
N

H
x
Q

A

V

I

Y

G

G

M

-

Y

-

P

S

V

I

D

D

Q

S

Q

G

L

S

S

W

T

T

D

E

C

S

E

S

-

P

F

L

R

E

P

P

D

N

G

S

F

P

Y
|
Y

G

A

L

A

S

S

K
|
K

M

A

W

G

G

S

E

D

G

L

L

V

A

A

R

R

L

A

Y

Y

W

H

D

R

K

T

H

Y

G

G

I

L

E

D

S

-

V

-

C

-

V

V

R

R

I

I

G

T

S

R

C

C

V

C

E
x
N

R
x
N

P

Y

Q

G

E

P

F

Y

R

Q

H

H

L

-

S

-

T

-

W

-

L

-

G

-

H

P

E

E

D
x
K

L
|
L

V

I

H

P

L

L

I

F

E

V

Q

T

G

N

I

L

T

L

V

-

P

-

D

D

L

G

G

G

F

T

L

L

V
x
P

V

L

W
x
Y

G

G

V

D

S

G

A

A

N

N

A

V

R
|
R

S

E

C

W

W

V

N

H

N

T

D

D

G

D

A

H

A

C

R

R

active site: T127 (≠ S110), N128 (= N111), Q129 (≠ H112), Y151 (= Y135), K155 (= K139)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S87 (= S74), H88 (vs. gap), T127 (≠ S110), N128 (= N111), Q129 (≠ H112), Y151 (= Y135), N180 (≠ E167), K190 (≠ D183), L191 (= L184), P206 (≠ V201), Y208 (≠ W203), R215 (= R210)
binding nicotinamide-adenine-dinucleotide: G10 (= G11), F11 (≠ Q12), I12 (≠ L13), D37 (vs. gap), S38 (≠ D27), L39 (= L28), T40 (≠ R29), A42 (≠ S31), G43 (= G32), D63 (= D50), I64 (≠ L51), F83 (≠ M70), A84 (= A71), A85 (≠ G72), S87 (= S74), T102 (≠ N85), V125 (≠ A108), S126 (= S109), Y151 (= Y135), K155 (= K139), N181 (≠ R168)

Sites not aligning to the query:

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: 250, 274, 277, 281

1r66A Crystal structure of desiv (dtdp-glucose 4,6-dehydratase) from streptomyces venezuelae with NAD and tyd bound (see paper)
26% identity, 80% coverage: 3:220/272 of query aligns to 2:225/322 of 1r66A

query
sites
1r66A

K

R

I

L

A

L

L

V

S

T

G

G

A

G

G

A

G
|
G

Q
x
F

L
x
I

G

G

T

S

V

H

L

F

R

V

T

R

A

Q

L

L

A

A

R

G

G

A

Y

Y

-

P

-

D

-

V

-

P

-

A

-

D

-

E

-

V

-

I

-

V

-

L

-
x
D

D
x
S

L
|
L

R
x
T

S

Y

S

A

G
|
G

N

N

S

R

Q

A

S

N

L

L

A

A

P

P

V

V

A

D

E

A

G

D

E

P

E

R

V

L

-

R

-

F

M

V

R

H

G

G

D
|
D

L
x
I

C

R

D

D

P

A

A

G

V

L

D

A

R

R

L

E

L

L

D

R

G

G

V

V

D

D

V

A

L

I

I

V

H

H

M
x
F

A
|
A

G
x
A

T

E

S
|
S

-
x
H

V

V

E

D

R

R

P

S

L

I

-

A

-

G

-

A

P

S

E

V

I

F

I

T

D

E

N
x
T

N

N

L

V

R

Q

A

G

L

T

V

Q

E

T

V

L

Y

L

E

Q

G

C

A

A

R

V

R

D

Q

A

K

G

V

V

G

G

R

R

I

V

V

V

F

H

A
x
V

S
|
S

S
x
T

N
x
D

H
x
E

A

V

I

Y

G

G

M

-

Y

-

P

S

V

I

D

D

Q

S

Q

G

L

S

S

W

T

T

D

E

C

S

E

S

-

P

F

L

R

E

P

P

D

N

G

S

F

P

Y
|
Y

G

A

L

A

S

S

K
|
K

M

A

W

G

G

S

E

D

G

L

L

V

A

A

R

R

L

A

Y

Y

W

H

D

R

K

T

H

Y

G

G

I

L

E

D

S

-

V

-

C

-

V

V

R

R

I

I

G

T

S

R

C

C

V

C

E
x
N

R
x
N

P

Y

Q

G

E

P

F

Y

R

Q

H

H

L

-

S

-

T

-

W

-

L

-

G

-

H

P

E

E

D
x
K

L
|
L

V

I

H

P

L

L

I

F

E

V

Q

T

G

N

I

L

T

L

V

-

P

-

D

D

L

G

G

G

F

T

L

L

V
x
P

V

L

W
x
Y

G

G

V

D

S

G

A

A

N

N

A

V

R
|
R

S

E

C

W

W

V

N

H

N

T

D

D

G

D

A

H

A

C

R

R

active site: T127 (≠ S110), D128 (≠ N111), E129 (≠ H112), Y151 (= Y135), K155 (= K139)
binding nicotinamide-adenine-dinucleotide: G10 (= G11), F11 (≠ Q12), I12 (≠ L13), D37 (vs. gap), S38 (≠ D27), L39 (= L28), T40 (≠ R29), G43 (= G32), D63 (= D50), I64 (≠ L51), F83 (≠ M70), A84 (= A71), A85 (≠ G72), S87 (= S74), T102 (≠ N85), V125 (≠ A108), S126 (= S109), Y151 (= Y135), K155 (= K139), N181 (≠ R168)
binding thymidine-5'-diphosphate: H88 (vs. gap), E129 (≠ H112), N180 (≠ E167), K190 (≠ D183), L191 (= L184), P206 (≠ V201), Y208 (≠ W203), R215 (= R210)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 250, 274, 277

7eprB Partial consensus l-threonine 3-dehydrogenase (c-change) (see paper)
30% identity, 58% coverage: 3:161/272 of query aligns to 3:166/310 of 7eprB

query
sites
7eprB

K

K

I

I

A

L

L

I

S

V

G
|
G

A

A

G

C

G
|
G

Q
|
Q

L
x
I

G

G

T

S

V

E

L

L

R

A

T

L

A

A

L

L

L

A

A

E

R

R

-

Y

-

G

-

N

-

T

-

N

-

V

-

I

G

T

Y

S

D
|
D

L
x
I

R
|
R

S

E

S

G

G

G

N

R

S

H

Q

N

S

S

L

-

A

-

P

-

V

-

A

-

E

-

G

G

E

T

E

H

V

E

M

M

-

L

-
x
D

-
x
A

-

T

-

D

R

R

G

G

D

E

L

L

C

A

D

-

P

-

A

T

V

V

D

E

R

R

-

H

L

K

L

I

D

T

G

Q

V

V

D

Y

V

L

L
|
L

I
x
A

H
x
A

M

L

A

L

G

S

T

A

S

T

V

G

E

E

R

K

P

N

L

P

P

Q

E

W

I

A

I

W

D

N

N
x
L

N

N

L

M

R

T

A

S

L

L

V

L

E

N

V

V

Y

L

E

E

G

L

A

A

R

R

R

Q

Q

T

K

K

V

I

G

K

R

R

I

V

V

F

F

W

A
x
P

S

S

N

I

H

A

A

V

I

F

G

G

M

P

Y

T

P

T

V

P

D

K

Q

E

Q

N

L

T

S

P

T

Q

D

Y

C

T

E

V

F

M

R

E

P

P

D

S

G

T

F

V

Y
|
Y

G

G

L

I

S

S

K
|
K

M

Q

W

A

G

G

E

E

G

G

L

W

A

C

R

R

L

W

Y

Y

W

H

D

A

K

N

H

H

G

G

I

V

E

D

S

V

V

R

C

S

V

V

R

R

binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), Q12 (= Q12), I13 (≠ L13), D34 (= D27), I35 (≠ L28), R36 (= R29), D50 (vs. gap), A51 (vs. gap), L72 (= L67), A73 (≠ I68), A74 (≠ H69), L90 (≠ N85), P113 (≠ A108), Y140 (= Y135), K144 (= K139)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 167, 170

6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
28% identity, 59% coverage: 3:162/272 of query aligns to 2:170/307 of 6wjaA

query
sites
6wjaA

K

R

I

I

A

L

L

V

S

T

G
|
G

A

G

G

A

G
|
G

Q
x
F

L
x
I

G

G

T

S

V

H

L

L

R

V

T

D

A

A

L

L

A

A

R

K

G

G

Y

Y

D

A

L

V

R

R

-

V

-

L

-
x
D

-

L

S
|
S

S
x
T

G
|
G

N

K

S

V

Q

G

S

N

L

L

A

P

P

M

V

G

A

D

E

A

G

G

E

L

E

E

V

L

M

L

R

V

G

G

D

D

L
x
A

C

A

D

D

P

A

A

A

V

L

D

A

R

D

L

A

L

V

D

Q

G

G

V

C

D

D

V

A

L

V

I

V

H

H

M
x
L

A
|
A

G
x
A

T

V

-

A

-

S

-
x
V

-

Q

-

A

S

S

V

V

E

E

R

D

P

P

L

V

P

-

E

A

I

T

I

H

D

Q

N
x
S

N

N

L

F

R

I

A

A

L

T

V

L

E

R

V

L

Y

C

E

E

G

A

A

M

R

T

R

A

Q

A

K

G

V

I

G

R

R

R

I

V

V

V

F

F

A

A

S

S

S
x
A

N
x
A

H
x
A

A

V

I

Y

G

G

M

N

Y

N

P

G

V

E

D

G

Q

T

Q

P

L

I

S

A

T

E

D

D

C

T

E

P

F

K

R

S

P

P

D

L

G

T

F

P

Y
x
F

G

A

L

A

S

D

K
|
K

M

L

W

A

G

S

E

E

G

Y

L

Y

A

L

R

D

L

F

Y

Y

W

R

D

R

K

Q

H

H

G

G

I

L

E

E

S

P

V

V

C

I

V

L

R

R

I
x
F

active site: A118 (≠ S110), A119 (≠ N111), A120 (≠ H112), F143 (≠ Y135), K147 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (≠ Q12), I12 (≠ L13), D31 (vs. gap), D32 (vs. gap), S34 (= S30), T35 (≠ S31), G36 (= G32), A55 (≠ L51), L74 (≠ M70), A75 (= A71), A76 (≠ G72), S93 (≠ N85), F143 (≠ Y135), K147 (= K139), F170 (≠ I162)
binding uridine-diphosphate-n-acetylgalactosamine: V80 (vs. gap), A120 (≠ H112)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 171, 173
binding uridine-diphosphate-n-acetylgalactosamine: 172, 186, 187, 191, 202, 204, 211, 247, 270, 273

6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
28% identity, 59% coverage: 3:162/272 of query aligns to 3:171/308 of 6wj9B

query
sites
6wj9B

K

R

I

I

A

L

L

V

S

T

G
|
G

A

G

G

A

G
|
G

Q
x
F

L
x
I

G

G

T

S

V

H

L

L

R

V

T

D

A

A

L

L

A

A

R

K

G

G

Y

Y

D

A

L

V

R

R

-

V

-

L

-
x
D

-

L

S
|
S

S
x
T

G
|
G

N

K

S

V

Q

G

S

N

L

L

A

P

P

M

V

G

A

D

E

A

G

G

E

L

E

E

V

L

M

L

R

V

G

G

D
|
D

L
x
A

C

A

D

D

P

A

A

A

V

L

D

A

R

D

L

A

L

V

D

Q

G

G

V

C

D

D

V

A

L

V

I

V

H

H

M
x
L

A
|
A

G
x
A

T

V

-

A

-

S

-
x
V

-

Q

-

A

S

S

V

V

E

E

R

D

P

P

L

V

P

-

E

A

I

T

I

H

D

Q

N
x
S

N

N

L

F

R

I

A

A

L

T

V

L

E

R

V

L

Y

C

E

E

G

A

A

M

R

T

R

A

Q

A

K

G

V

I

G

R

R

R

I

V

V

V

F

F

A
|
A

S

S

S
x
A

N
x
A

H
x
A

A

V

I

Y

G

G

M

N

Y

N

P

G

V

E

D

G

Q

T

Q

P

L

I

S

A

T

E

D

D

C

T

E

P

F

K

R

S

P

P

D

L

G

T

F

P

Y
x
F

G

A

L

A

S

D

K
|
K

M

L

W

A

G

S

E

E

G

Y

L

Y

A

L

R

D

L

F

Y

Y

W

R

D

R

K

Q

H

H

G

G

I

L

E

E

S

P

V

V

C

I

V

L

R

R

I
x
F

active site: A119 (≠ S110), A120 (≠ N111), A121 (≠ H112), F144 (≠ Y135), K148 (= K139)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), F12 (≠ Q12), I13 (≠ L13), D32 (vs. gap), D33 (vs. gap), S35 (= S30), T36 (≠ S31), G37 (= G32), D55 (= D50), A56 (≠ L51), L75 (≠ M70), A76 (= A71), A77 (≠ G72), S94 (≠ N85), A117 (= A108), A119 (≠ S110), F144 (≠ Y135), K148 (= K139), F171 (≠ I162)
binding uridine-diphosphate-n-acetylglucosamine: V81 (vs. gap)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 172, 174
binding uridine-diphosphate-n-acetylglucosamine: 173, 187, 188, 192, 203, 204, 205, 212, 248, 271, 274

5z75A Artificial l-threonine 3-dehydrogenase designed by ancestral sequence reconstruction. (see paper)
28% identity, 59% coverage: 1:161/272 of query aligns to 2:166/306 of 5z75A

query
sites
5z75A

M

M

T

M

K

K

I

I

A

L

L

V

S

I

G
|
G

A

A

G
x
C

G
|
G

Q
|
Q

L
x
I

G

G

T

T

V

E

L

L

R

-

T

T

A

V

L

A

L

L

A

R

R

E

G

I

Y

Y

D

G

L

N

R

E

S

N

S

V

G

V

N

A

S

S

Q
x
D

S
x
I

L

R

A

E

P

P

V

N

A

E

E

E

G

S

E

G

E

P

V

F

M

E

R

K

G
x
L

D

D

L
x
V

C

M

D

D

P

K

A

E

V

R

V

L

D

E

R

E

L

I

L

V

D

E

G

K

-

H

-

K

V

I

D

T

V

Q

L

V

I

Y

H

H

M
x
L

A
|
A

G

A

-

I

-
x
L

-

S

-

A

T

T

S

G

V

E

E

K

R

N

P

P

L

L

P

-

E

F

I

A

I

W

D

D

N

L

N

N

L

M

R

N

A

S

L

L

V

L

E

N

V

V

Y

L

E

E

G

L

A

A

R

R

R

E

Q

G

K

K

V

I

G

D

R

K

I

I

V

F

F

W

A
x
P

S

S

S
|
S

N

I

H

A

A

V

I

F

G

G

M

P

Y

T

P

T

V

P

D

K

Q

E

Q

N

L

T

S

P

T

Q

D

H

C

T

E

V

F

M

R

D

P

P

D

S

G

T

F

V

Y
|
Y

G

G

L

I

S

S

K
|
K

M

L

W

A

G

G

E

E

G

R

L

W

A

C

R

E

L

Y

Y

Y

W

H

D

E

K

K

H

Y

G

G

I

V

E

D

S

V

V

R

C

S

V

I

R

R

binding nicotinamide-adenine-dinucleotide: G9 (= G8), C11 (≠ G10), G12 (= G11), Q13 (= Q12), I14 (≠ L13), D35 (≠ Q35), I36 (≠ S36), L49 (≠ G49), V51 (≠ L51), L72 (≠ M70), A73 (= A71), L76 (vs. gap), P113 (≠ A108), S115 (= S110), Y140 (= Y135), K144 (= K139)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 167, 168, 170, 176, 177

7epsA Partial consensus l-threonine 3-dehydrogenase (e-change) (see paper)
30% identity, 58% coverage: 3:161/272 of query aligns to 2:165/310 of 7epsA

query
sites
7epsA

K

K

I

I

A

L

L

I

S

I

G

G

A

A

G
x
N

G
|
G

Q
|
Q

L
x
I

G

G

T

S

V

E

L

L

R

A

T

L

A

A

L

L

L

A

A

E

R

R

-

Y

-

G

-

R

-

E

-

N

-

V

-

I

G

A

Y

S

D
|
D

L
x
V

R

V

S

P

S

T

G

G

N

R

S

H

Q

V

S

H

L

L

A

-

P

P

V

F

A

E

E

V

G
x
L

E

N

E
x
A

V

T

M

D

R

R

G

G

D

E

L

L

C

A

D

-

P

-

A

T

V

V

D

E

R

R

L

H

L

G

D

I

G

T

V

Q

D

V

V

Y

L

L

I
x
L

H
x
A

M
x
A

A

A

G

L

T
x
S

S

A

V

T

E

G

R

E

P

K

L

A

P

P

E

Q

I

W

I

A

D

W

N

N

-
x
L

N

N

L

M

R

T

A

S

L

L

V

L

E

N

V

V

Y

L

E

E

G

L

A

A

R

R

R

Q

Q

T

K

G

V

L

G

E

R

K

I

I

V

F

F

W

A
x
P

S

S

S
|
S

N

I

H

A

A

A

I

F

G

G

M

P

Y

T

P

T

V

P

D

A

Q

G

Q

Q

L

T

S

P

T

Q

D

K

C

T

E

V

F

M

R

E

P

P

D

T

G

T

F

V

Y
|
Y

G

G

L

I

S

S

K
|
K

M

Q

W

A

G

G

E

E

G

G

L

W

A

C

R

R

L

W

Y

Y

W

H

D

A

K

N

H

H

G

G

I

V

E

D

S

V

V

R

C

S

V

I

R

R

binding nicotinamide-adenine-dinucleotide: N9 (≠ G10), G10 (= G11), Q11 (= Q12), I12 (≠ L13), D33 (= D27), V34 (≠ L28), L48 (≠ G43), A50 (≠ E45), L71 (≠ I68), A72 (≠ H69), A73 (≠ M70), L89 (vs. gap), P112 (≠ A108), Y139 (= Y135), K143 (= K139)
binding threonine: S76 (≠ T73), S114 (= S110), Y139 (= Y135)

Sites not aligning to the query:

3a1nA Crystal structure of l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
23% identity, 62% coverage: 4:171/272 of query aligns to 2:173/315 of 3a1nA

query
sites
3a1nA

I

I

A

L

L

V

S

T

G

G

A

S

G
x
S

G
|
G

Q
|
Q

L
x
I

G

G

T

T

V

E

L

L

R

-

T

V

A

P

L

Y

L

L

A

A

R

E

G

K

Y

Y

D

G

L

K

R

K

S

N

S

V

G

I

N

A

S

S

Q
x
D

S
x
I

L

V

A

Q

P

R

V

D

A

T

E

G

G

G

E

I

E

K

V

F

M

I

R

T

G
x
L

D
|
D

L

V

C

S

D

N

P

R

A

D

V

E

V

I

D

D

R

R

L

A

L

V

D

E

-

K

-

Y

G

S

V

I

D

D

V

A

L

I

I

F

H

H

M
x
L

A
|
A

G
|
G

-

I

-
x
L

-

S

T

A

S

K

V

G

E

E

R

K

P

D

L

P

P

A

E

L

I

A

I

Y

D

K

N

V

N

N

L

M

R

N

A

G

L

T

V

Y

E

N

V

I

Y

L

E

E

G

A

A

A

R

K

R

Q

Q

H

K

R

V

V

G

E

R

K

I

V

V

V

F

I

A

P

S
|
S

S
x
T

N

I

H

G

A

V

I

F

G

G

M

P

Y

E

P

T

V

P

D

K

Q

N

Q

K

L

V

S

P

T

S

D

I

C

T

E

I

F

T

R

R

P

P

D

R

G

T

F

M

Y
|
Y

G

G

L

V

S

T

K
|
K

M

I

W

A

G

A

E

E

G

L

L

L

A

G

R

Q

L

Y

Y

Y

W

Y

D

E

K

K

H

F

G

G

I

L

E

D

S

V

V

R

C

S

V

L

R

R

I
x
Y

G

P

S

G

C

I

V

I

E

S

R

Y

P

K

Q

A

E

E

active site: T112 (≠ S110), Y137 (= Y135), K141 (= K139)
binding nicotinamide-adenine-dinucleotide: S8 (≠ G10), G9 (= G11), Q10 (= Q12), I11 (≠ L13), D32 (≠ Q35), I33 (≠ S36), L46 (≠ G49), D47 (= D50), L69 (≠ M70), A70 (= A71), G71 (= G72), L73 (vs. gap), S111 (= S109), Y137 (= Y135), K141 (= K139), Y164 (≠ I162)

3a4vA Crystal structure of pyruvate bound l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
23% identity, 62% coverage: 4:171/272 of query aligns to 2:173/311 of 3a4vA

query
sites
3a4vA

I

I

A

L

L

V

S

T

G

G

A

S

G
x
S

G
|
G

Q
|
Q

L
x
I

G

G

T

T

V

E

L

L

R

-

T

V

A

P

L

Y

L

L

A

A

R

E

G

K

Y

Y

D

G

L

K

R

K

S

N

S

V

G

I

N

A

S

S

Q
x
D

S
x
I

L

V

A

Q

P

R

V

D

A

T

E

G

G

G

E

I

E

K

V

F

M

I

R

T

G
x
L

D
|
D

L
x
V

C

S

D

N

P

R

A

D

V

E

V

I

D

D

R

R

L

A

L

V

D

E

-

K

-

Y

G

S

V

I

D

D

V

A

L

I

I

F

H

H

M
x
L

A
|
A

G
|
G

-

I

-
x
L

-
x
S

T

A

S

K

V

G

E

E

R

K

P

D

L

P

P

A

E

L

I

A

I

Y

D

K

N

V

N

N

L

M

R

N

A

G

L

T

V

Y

E

N

V

I

Y

L

E

E

G

A

A

A

R

K

R

Q

Q

H

K

R

V

V

G

E

R

K

I

V

V

V

F

I

A
x
P

S
|
S

S
x
T

N

I

H

G

A

V

I

F

G

G

M

P

Y

E

P

T

V

P

D

K

Q

N

Q

K

L

V

S

P

T

S

D

I

C

T

E

I

F

T

R

R

P

P

D

R

G

T

F

M

Y
|
Y

G

G

L

V

S

T

K
|
K

M

I

W

A

G

A

E

E

G

L

L

L

A

G

R

Q

L

Y

Y

Y

W

Y

D

E

K

K

H

F

G

G

I

L

E

D

S

V

V

R

C

S

V

L

R

R

I
x
Y

G

P

S

G

C
x
I

V

I

E

S

R

Y

P

K

Q

A

E

E

active site: T112 (≠ S110), Y137 (= Y135), K141 (= K139)
binding nicotinamide-adenine-dinucleotide: S8 (≠ G10), G9 (= G11), Q10 (= Q12), I11 (≠ L13), D32 (≠ Q35), I33 (≠ S36), L46 (≠ G49), D47 (= D50), V48 (≠ L51), L69 (≠ M70), A70 (= A71), G71 (= G72), L73 (vs. gap), P110 (≠ A108), S111 (= S109), T112 (≠ S110), Y137 (= Y135), K141 (= K139), Y164 (≠ I162), I167 (≠ C165)
binding pyruvic acid: S74 (vs. gap), Y137 (= Y135)

Sites not aligning to the query:

binding pyruvic acid: 178, 179, 273

2udpA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
28% identity, 53% coverage: 3:145/272 of query aligns to 2:159/338 of 2udpA

query
sites
2udpA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

T

S

V

H

L

T

R

C

T

V

A

Q

L

L

A

Q

R

N

G

G

Y

H

D

D

-

V

-

I

L

L

R
x
D

S
x
N

S
x
L

G
x
C

N
|
N

S
|
S

Q

K

-

R

S

S

L

V

A

L

P

P

V

V

A

I

E

E

-

R

-

L

-

G

-

K

G

H

E

P

E

T

V

F

M

V

R

E

G

G

D
|
D

L
x
I

C

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

D

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

M
x
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

D

D

N

N

L

V

R

N

A

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

K

N

V

V

G

K

R

N

I

F

V

I

F

F

A
x
S

S

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

Q

P

P

V

K

D

I

Q

P

Q

Y

L

V

S

E

T

S

D

F

C

P

E

T

F

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

M

L

W

M

G

V

E

E

G

Q

L

I

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), L33 (≠ S31), C34 (≠ G32), N35 (= N33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (≠ M70), A81 (= A71), G82 (= G72), K84 (vs. gap), S122 (≠ A108), S124 (= S110), Y149 (= Y135), K153 (= K139)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177
binding phenyl-uridine-5'-diphosphate: 179, 199, 200, 216, 217, 218, 231, 233, 269, 292, 295

1udcA Structure of udp-galactose-4-epimerase complexed with udp-mannose (see paper)
28% identity, 53% coverage: 3:145/272 of query aligns to 2:159/338 of 1udcA

query
sites
1udcA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

T

S

V

H

L

T

R

C

T

V

A

Q

L

L

A

Q

R

N

G

G

Y

H

D

D

-

V

-

I

L

L

R
x
D

S
x
N

S

L

G
x
C

N
|
N

S
|
S

Q

K

-

R

S

S

L

V

A

L

P

P

V

V

A

I

E

E

-

R

-

L

-

G

-

K

G

H

E

P

E

T

V

F

M

V

R

E

G

G

D
|
D

L
x
I

C

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

D

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

M
x
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

D

D

N

N

L

V

R

N

A

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

K

N

V

V

G

K

R

N

I

F

V

I

F

F

A
x
S

S

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

Q

P

P

V

K

D

I

Q

P

Q

Y

L

V

S

E

T

S

D

F

C

P

E

T

F

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

M

L

W

M

G

V

E

E

G

Q

L

I

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), C34 (≠ G32), N35 (= N33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (≠ M70), A81 (= A71), G82 (= G72), K84 (vs. gap), S122 (≠ A108), Y149 (= Y135), K153 (= K139)
binding uridine-5'-diphosphate-mannose: T126 (≠ H112), Y149 (= Y135)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-5'-diphosphate-mannose: 179, 199, 200, 215, 216, 217, 218, 231, 233, 269, 292

P09147 UDP-glucose 4-epimerase; Galactowaldenase; UDP-galactose 4-epimerase; EC 5.1.3.2 from Escherichia coli (strain K12) (see 10 papers)
28% identity, 53% coverage: 3:145/272 of query aligns to 2:159/338 of P09147

query
sites
P09147

K

R

I

V

A

L

L

V

S

T

G

G

A

G

G

S

G

G

Q
x
Y

L
x
I

G

G

T

S

V

H

L

T

R

C

T

V

A

Q

L

L

A

Q

R

N

G

G

Y

H

D

D

-

V

-

I

L

L

R
x
D

S
x
N

S
x
L

G
x
C

N
|
N

S
|
S

Q

K

-

R

S

S

L

V

A

L

P

P

V

V

A

I

E

E

-

R

-

L

-

G

-

K

G

H

E

P

E

T

V

F

M

V

R

E

G

G

D
|
D

L
x
I

C

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

D

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

M
x
F

A
|
A

G
|
G

-
x
L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

D

D

N
|
N

N

N

L

V

R

N

A

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

K

N

V

V

G

K

R

N

I

F

V

I

F

F

A

S

S

S

S
|
S

N

A

H

T

A

V

I

Y

G

G

M

D

Y

Q

P

P

V

K

D

I

Q

P

Q

Y

L

V

S

E

T

S

D

F

C

P

E

T

F

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

M

L

W

M

G

V

E

E

G

Q

L

I

YI 11:12 (≠ QL 12:13) binding
DNLCNS 31:36 (≠ RSSGNS 29:34) binding
DI 58:59 (≠ DL 50:51) binding
FAGLK 80:84 (≠ MAG-- 70:72) binding
N99 (= N85) binding
S124 (= S110) binding ; mutation to A: No major structural changes. Catalytic efficiency is very low and affinity binding is 21% of the wild-type enzyme.; mutation to T: No major structural changes. Catalytic efficiency is about 30% of that of the wild-type enzyme, and affinity binding is similar to that of the native enzyme.
Y149 (= Y135) active site, Proton acceptor; binding ; mutation to F: No major structural changes. Catalytic efficiency is very low and affinity binding is 12% of the wild-type enzyme.
K153 (= K139) binding ; mutation to A: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.; mutation to M: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.

Sites not aligning to the query:

178 binding
299 Y→C: Loss of epimerase activity with UDP-Gal by almost 5-fold, but it results in a gain of epimerase activity with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by 230-fold with minimal changes in its three-dimensional structure.

1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
28% identity, 53% coverage: 3:145/272 of query aligns to 2:159/338 of 1udaA

query
sites
1udaA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

T

S

V

H

L

T

R

C

T

V

A

Q

L

L

A

Q

R

N

G

G

Y

H

D

D

-

V

-

I

L

L

R
x
D

S
x
N

S
x
L

G
x
C

N
|
N

S
|
S

Q

K

-

R

S

S

L

V

A

L

P

P

V

V

A

I

E

E

-

R

-

L

-

G

-

K

G

H

E

P

E

T

V

F

M

V

R

E

G

G

D
|
D

L
x
I

C

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

D

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

M
x
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

D

D

N

N

L

V

R

N

A

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

K

N

V

V

G

K

R

N

I

F

V

I

F

F

A
x
S

S

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

N

P

P

V

K

D

I

Q

P

Q

Y

L

V

S

E

T

S

D

F

C

P

E

T

F

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

M

L

W

M

G

V

E

E

G

Q

L

I

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), L33 (≠ S31), C34 (≠ G32), N35 (= N33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (≠ M70), A81 (= A71), G82 (= G72), K84 (vs. gap), S122 (≠ A108), Y149 (= Y135), K153 (= K139)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: T126 (≠ H112)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: 179, 199, 200, 216, 217, 218, 231, 233, 292, 295, 299

1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
28% identity, 53% coverage: 3:145/272 of query aligns to 2:159/338 of 1naiA

query
sites
1naiA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

T

S

V

H

L

T

R

C

T

V

A

Q

L

L

A

Q

R

N

G

G

Y

H

D

D

-

V

-

I

L

L

R
x
D

S
x
N

S
x
L

G
x
C

N
|
N

S
|
S

Q

K

-

R

S

S

L

V

A

L

P

P

V

V

A

I

E

E

-

R

-

L

-

G

-

K

G

H

E

P

E

T

V

F

M

V

R

E

G

G

D
|
D

L
x
I

C

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

D

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

M
x
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

D

D

N

N

L

V

R

N

A

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

K

N

V

V

G

K

R

N

I

F

V

I

F

F

A

S

S

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

N

P

P

V

K

D

I

Q

P

Q

Y

L

V

S

E

T

S

D

F

C

P

E

T

F

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

M

L

W

M

G

V

E

E

G

Q

L

I

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), L33 (≠ S31), C34 (≠ G32), N35 (= N33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (≠ M70), A81 (= A71), G82 (= G72), K84 (vs. gap), Y149 (= Y135), K153 (= K139)
binding 1,3-propandiol: N35 (= N33), K84 (vs. gap)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding 1,3-propandiol: 191, 193
binding uridine-5'-diphosphate: 179, 199, 200, 215, 216, 231, 233, 292

Query Sequence

>RR42_RS11300 FitnessBrowser__Cup4G11:RR42_RS11300
MTKIALSGAGGQLGTVLRTALLARGYDLRSSGNSQSLAPVAEGEEVMRGDLCDPAVVDRL
LDGVDVLIHMAGTSVERPLPEIIDNNLRALVEVYEGARRQKVGRIVFASSNHAIGMYPVD
QQLSTDCEFRPDGFYGLSKMWGEGLARLYWDKHGIESVCVRIGSCVERPQEFRHLSTWLG
HEDLVHLIEQGITVPDLGFLVVWGVSANARSCWNNDGAARLRYRPKQNAEDFAEEILAGP
NPLDAIGRQYQGGSFASIDFTPEAQRHGGAHR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory