SitesBLAST

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
25% identity, 82% coverage: 28:158/159 of query aligns to 42:182/203 of P07464

query
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P07464

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S71 (≠ D57) binding
N85 (≠ S71) binding in other chain; binding in other chain
D93 (≠ S78) binding
H115 (= H91) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S118) binding in other chain
A160 (= A136) binding in other chain
TK 165:166 (≠ RG 141:142) binding
R180 (= R156) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
183 binding in other chain

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
26% identity, 73% coverage: 41:156/159 of query aligns to 86:217/259 of 3fscA

query
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3fscA

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binding coenzyme a: F150 (vs. gap), A178 (≠ G117), A179 (≠ S118), G196 (= G135), A197 (= A136), V212 (= V151)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (≠ Q69), G114 (≠ S71), T115 (≠ S72), H132 (vs. gap), S133 (vs. gap)

Sites not aligning to the query:

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d- glucose n-acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
26% identity, 73% coverage: 41:156/159 of query aligns to 86:217/259 of 3fs8A

query
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3fs8A

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binding acetyl coenzyme *a: F150 (vs. gap), P151 (vs. gap), A178 (≠ G117), A179 (≠ S118), G196 (= G135), A197 (= A136), V212 (= V151)
binding thymine: R102 (≠ D57)

Sites not aligning to the query:

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
26% identity, 73% coverage: 41:156/159 of query aligns to 86:217/260 of 3fsbA

query
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3fsbA

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binding coenzyme a: F150 (vs. gap), A178 (≠ G117), A179 (≠ S118), G196 (= G135), A197 (= A136), V212 (= V151)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: G114 (≠ S71), H132 (vs. gap), F150 (vs. gap)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 241, 243

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
37% identity, 69% coverage: 50:158/159 of query aligns to 142:233/233 of 4n6bA

query
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4n6bA

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binding coenzyme a: H150 (≠ A58), L169 (= L77), G176 (= G89), K208 (≠ V133), G210 (= G135), A211 (= A136), L216 (≠ R141), V226 (= V151)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
34% identity, 69% coverage: 50:158/159 of query aligns to 149:243/243 of 4n69A

query
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4n69A

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binding serine: G183 (≠ T97), R191 (≠ Q105), H192 (≠ A106)

2fkoA Structure of ph1591 from pyrococcus horikoshii ot3 (see paper)
45% identity, 31% coverage: 110:158/159 of query aligns to 91:141/173 of 2fkoA

query
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2fkoA

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Sites not aligning to the query:

active site: 47, 59, 65, 68, 82, 87, 161
binding zinc ion: 65, 87

1v3wA Structure of ferripyochelin binding protein from pyrococcus horikoshii ot3 (see paper)
45% identity, 31% coverage: 110:158/159 of query aligns to 91:141/173 of 1v3wA

query
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1v3wA

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Sites not aligning to the query:

active site: 47, 59, 65, 68, 82, 87, 161
binding calcium ion: 40, 61
binding zinc ion: 65, 87

7l7yAAA Putative acetyl transferase protein (see paper)
44% identity, 31% coverage: 110:159/159 of query aligns to 163:217/217 of 7l7yAAA

query
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7l7yAAA

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binding acetyl coenzyme *a: Y168 (≠ F115), G170 (= G117), T171 (≠ S118), G193 (= G135), M194 (≠ A136), I209 (≠ V151)

Sites not aligning to the query:

binding uridine-5'-diphosphate: 10, 12, 39, 40, 73, 74

7l82AAA Putative acetyl transferase protein (see paper)
44% identity, 31% coverage: 110:158/159 of query aligns to 163:216/216 of 7l82AAA

query
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7l82AAA

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binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: Y168 (≠ F115), T171 (≠ S118), G193 (= G135), M194 (≠ A136), I209 (≠ V151)

Sites not aligning to the query:

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: 10, 12, 39, 40, 73, 74

7l81AAA Putative acetyl transferase protein (see paper)
44% identity, 31% coverage: 110:158/159 of query aligns to 163:216/216 of 7l81AAA

query
sites
7l81AAA

I

I

G

H

D

D

F

H

T

A

F
x
Y

V

I

G

G

S
x
T

G

G

A

A

I

V

I

I

L

K

P

Q

G

G

T

T

R

P

-

D

-

K

-

P

-

L

-

I

I

I

G

G

K

K

G

G

C

A

V

I

I

V

G
|
G

A
x
M

G

G

S

A

V

V

R

T

G

K

E

E

I

V

P

P

D

A

H

G

S

A

V

V

V
x
I

G

G

N

N

P

P

G

A

R

R

I

L

V

L

binding coenzyme a: Y168 (≠ F115), T171 (≠ S118), G193 (= G135), M194 (≠ A136), I209 (≠ V151)

Sites not aligning to the query:

Query Sequence

>RR42_RS15740 FitnessBrowser__Cup4G11:RR42_RS15740
MNLLGMLLRPFMVYGYFSRREGRFLRFTRVSSSAKILSERDLVIGDHCWIGPHCLIDASG
GVEIGEGVQISSLNAVLSHSSHVSIRLLGRHFISTPTPQRTGFIQAPVAIGDFTFVGSGA
IILPGTRIGKGCVIGAGSVVRGEIPDHSVVVGNPGRIVG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory