SitesBLAST

D54 (≠ E25) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (≠ E28) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (≠ M80) mutation to A: Loss of transport activity.
W309 (= W297) mutation to V: Loss of transport activity.
D312 (= D300) mutation to A: Loss of transport activity.
R313 (= R301) mutation to A: Loss of transport activity.

Sites not aligning to the query:

317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

O42885 Putative inorganic phosphate transporter C8E4.01c from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
27% identity, 28% coverage: 69:221/546 of query aligns to 108:268/572 of O42885

query
sites
O42885

L

F

G

G

A

Q

L

L

V

L

F

F

G

G

R

F

I

M

G

G

D

D

M

F

I

F

G

G

R

R

K

K

R

F

T

V

F

Y

M

G

I

K

T

E

I

M

C

V

M

I

M

V

G

I

A

A

T

T

I

V

G

L

V

V

G

I

C

A

L

M

P

P

T

K

Y

S

A

I

S

H

A

S

G

P

I

L

V

S

S

K

P

M

I

M

A

W

L

V

L

F

C

C

L

W

R

R

I

W

L

L

Q

L

G

G

F

V

S

G

A

I

G

G

E

D

Y

Y

T

P

G

M

A

S

L

A

T

A

Y

I

V

T

A

S

E

E

Y

R

S

S

P

K

G

I

R

K

R

R

G

G

L

T

N

L

M

I

S

S

W

L

T

I

T

F

A

A

S

F

S

Q

T

G

V

F

G

G

L

T

L

L

L

A

S

G

F

A

L

I

V

V

I

T

L

I

A

I

S

L

R

L

T

G

I

C

A

F

G

E

D

H

S

P

F

L

D

N

D

R

W

E

G

G

-

H

-

Y

-

H

-

K

-

L

-

E

-

G

-

V

W

W

R

R

L

L

P

Q

F

F

L

G

A

L

A

A

S

L

V

V

M

P

L

A

L

I

V

G

S

V

L

L

L

I

L

P

R

R

V

L

R

I

M

M

D

K

E

E

S

S

P

K

A

S

F

Y

R

E

K

N

L

S

R

K

S

A

A

L

N

N

Sites not aligning to the query:

12 modified: Phosphoserine
14 modified: Phosphoserine
292 modified: Phosphoserine
296 modified: Phosphoserine

4gc0A The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to 6-bromo-6-deoxy-d-glucose (see paper)
25% identity, 36% coverage: 122:320/546 of query aligns to 129:353/475 of 4gc0A

query
sites
4gc0A

R

R

I

I

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

G

L

E

A

Y

S

T

M

G

L

A

S

L

P

T

M

Y

Y

V

I

A

A

E

E

Y

L

S

A

P

P

G

A

R

H

R

I

R

R

G

G

L

K

N

L

M

V

S

S

W

F

T

N

T
x
Q

A

F

S

A

S

I

T

I

V

F

G

G

L

Q

L

L

S

V

F

Y

L

C

V

V

-

N

I

Y

L

F

A

I

S

A

R

R

T

S

I

G

A

D

G

A

D

S

S

W

F

L

D

N

D

T

W

D

G

G

W

W

R

R

L

Y

P

M

F

F

L

A

A

S

A

E

S

C

V

I

M

P

L

A

L

L

V

L

S

F

L

L

L

M

L

L

R

L

V

Y

R

T

M

V

D

P

E

E

S

S

P

P

A

R

F

W

R

L

K

M

L

S

R

R

S

G

A

K

N

Q

K

E

L

Q

S

A

K

E

A

G

P

I

L

L

R

R

D

K

A

I

L

M

-

G

-

N

-

T

-

L

-

A

-

T

-

Q

-

A

-

V

-

Q

-

E

-

I

-

K

-

H

-

S

L

L

D

D

R

H

A

G

N

R

-

K

-

T

-

G

-

R

-

L

-

M

-

F

-

G

L

V

G

G

R

V

I

I

V

V

I

I

A

G

-

V

-

M

-

L

-

S

-

I

F

F

A
x
Q

L

Q

C

F

A

V

G

G

M

I

T
x
N

S

V

M

V

Y
x
L

Y

Y

M

Y

A

A

A

-

L

-

Y

-

P

P

T

E

F

V

F

F

L

K

T

T

K

L

S

G

L

A

K

S

V

T

D

D

P

I

T

A

T

L

V

L

N

Q

T

T

V

I

L

V

L

G

A

V

T

I

A

N

L

L

C

T

V

F

P

T

V

V

F

-

P

-

L

L

A

A

G

I

W

M

A

T

C

V

D

D

R

K

F

F

G

G

R

R

K

K

P

P

V

L

L

Q

L

I

V

I

G

G

F

A

L

L

T

G

S

M

A

A

L

I

L

G

A

M

F

F

binding 6-bromo-6-deoxy-beta-D-glucopyranose: Q164 (≠ T157), Q284 (≠ A246), N290 (≠ T252), L293 (≠ Y255)

Sites not aligning to the query:

binding 6-bromo-6-deoxy-beta-D-glucopyranose: 388

4gbzA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-glucose (see paper)
25% identity, 36% coverage: 122:320/546 of query aligns to 129:353/475 of 4gbzA

query
sites
4gbzA

R

R

I

I

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

G

L

E

A

Y

S

T

M

G

L

A

S

L

P

T

M

Y

Y

V

I

A

A

E

E

Y

L

S

A

P

P

G

A

R

H

R

I

R

R

G

G

L

K

N

L

M

V

S

S

W

F

T

N

T
x
Q

A

F

S

A

S

I

T

I

V

F

G

G

L

Q

L

L

S

V

F

Y

L

C

V

V

-

N

I

Y

L

F

A

I

S

A

R

R

T

S

I

G

A

D

G

A

D

S

S

W

F

L

D

N

D

T

W

D

G

G

W

W

R

R

L

Y

P

M

F

F

L

A

A

S

A

E

S

C

V

I

M

P

L

A

L

L

V

L

S

F

L

L

L

M

L

L

R

L

V

Y

R

T

M

V

D

P

E

E

S

S

P

P

A

R

F

W

R

L

K

M

L

S

R

R

S

G

A

K

N

Q

K

E

L

Q

S

A

K

E

A

G

P

I

L

L

R

R

D

K

A

I

L

M

-

G

-

N

-

T

-

L

-

A

-

T

-

Q

-

A

-

V

-

Q

-

E

-

I

-

K

-

H

-

S

L

L

D

D

R

H

A

G

N

R

-

K

-

T

-

G

-

R

-

L

-

M

-

F

-

G

L

V

G

G

R

V

I

I

V

V

I

I

A

G

-

V

-

M

-

L

-

S

-

I

F

F

A
x
Q

L

Q

C

F

A

V

G

G

M

I

T
x
N

S

V

M

V

Y

L

Y

Y

M

Y

A

A

A

-

L

-

Y

-

P

P

T

E

F

V

F

F

L

K

T

T

K

L

S

G

L

A

K

S

V

T

D

D

P

I

T

A

T

L

V

L

N

Q

T

T

V

I

L

V

L

G

A

V

T

I

A

N

L

L

C

T

V

F

P

T

V

V

F

-

P

-

L

L

A

A

G

I

W

M

A

T

C

V

D

D

R

K

F

F

G

G

R

R

K

K

P

P

V

L

L

Q

L

I

V

I

G

G

F

A

L

L

T

G

S

M

A

A

L

I

L

G

A

M

F

F

binding beta-D-glucopyranose: Q164 (≠ T157), Q284 (≠ A246), N290 (≠ T252)

Sites not aligning to the query:

binding beta-D-glucopyranose: 388

4gbyA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-xylose (see paper)
25% identity, 36% coverage: 122:320/546 of query aligns to 129:353/475 of 4gbyA

query
sites
4gbyA

R

R

I

I

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

G

L

E

A

Y

S

T

M

G

L

A

S

L

P

T

M

Y

Y

V

I

A

A

E

E

Y

L

S

A

P

P

G

A

R

H

R

I

R

R

G

G

L

K

N

L

M

V

S

S

W

F

T

N

T
x
Q

A

F

S

A

S

I

T

I

V

F

G

G

L

Q

L

L

S

V

F

Y

L

C

V

V

-

N

I

Y

L

F

A

I

S

A

R

R

T

S

I

G

A

D

G

A

D

S

S

W

F

L

D

N

D

T

W

D

G

G

W

W

R

R

L

Y

P

M

F

F

L

A

A

S

A

E

S

C

V

I

M

P

L

A

L

L

V

L

S

F

L

L

L

M

L

L

R

L

V

Y

R

T

M

V

D

P

E

E

S

S

P

P

A

R

F

W

R

L

K

M

L

S

R

R

S

G

A

K

N

Q

K

E

L

Q

S

A

K

E

A

G

P

I

L

L

R

R

D

K

A

I

L

M

-

G

-

N

-

T

-

L

-

A

-

T

-

Q

-

A

-

V

-

Q

-

E

-

I

-

K

-

H

-

S

L

L

D

D

R

H

A

G

N

R

-

K

-

T

-

G

-

R

-

L

-

M

-

F

-

G

L

V

G

G

R

V

I

I

V

V

I

I

A

G

-

V

-

M

-

L

-

S

-

I

F

F

A
x
Q

L

Q

C

F

A

V

G

G

M

I

T
x
N

S

V

M

V

Y

L

Y

Y

M

Y

A

A

A

-

L

-

Y

-

P

P

T

E

F

V

F

F

L

K

T

T

K

L

S

G

L

A

K

S

V

T

D

D

P

I

T

A

T

L

V

L

N

Q

T

T

V

I

L

V

L

G

A

V

T

I

A

N

L

L

C

T

V

F

P

T

V

V

F

-

P

-

L

L

A

A

G

I

W

M

A

T

C

V

D

D

R

K

F

F

G

G

R

R

K

K

P

P

V

L

L

Q

L

I

V

I

G

G

F

A

L

L

T

G

S

M

A

A

L

I

L

G

A

M

F

F

binding beta-D-xylopyranose: Q164 (≠ T157), Q284 (≠ A246), N290 (≠ T252)

Sites not aligning to the query:

binding beta-D-xylopyranose: 388

P0AGF4 D-xylose-proton symporter; D-xylose transporter from Escherichia coli (strain K12) (see paper)
25% identity, 36% coverage: 122:320/546 of query aligns to 133:357/491 of P0AGF4

query
sites
P0AGF4

R
|
R

I

I

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

G

L

E

A

Y

S

T

M

G

L

A

S

L

P

T

M

Y

Y

V

I

A

A

E
|
E

Y

L

S

A

P

P

G

A

R

H

R

I

R
|
R

G

G

L

K

N

L

M

V

S

S

W

F

T

N

T
x
Q

A

F

S

A

S

I

T

I

V

F

G

G

L

Q

L

L

S

V

F

Y

L

C

V

V

-

N

I

Y

L

F

A

I

S

A

R

R

T

S

I

G

A

D

G

A

D

S

S

W

F

L

D

N

D

T

W

D

G

G

W

W

R

R

L

Y

P

M

F

F

L

A

A

S

A

E

S

C

V

I

M

P

L

A

L

L

V

L

S

F

L

L

L

M

L

L

R

L

V

Y

R

T

M

V

D

P

E

E

S

S

P

P

A

R

F

W

R

L

K

M

L

S

R

R

S

G

A

K

N

Q

K

E

L

Q

S

A

K

E

A

G

P

I

L

L

R

R

D

K

A

I

L

M

-

G

-

N

-

T

-

L

-

A

-

T

-

Q

-

A

-

V

-

Q

-

E

-

I

-

K

-

H

-

S

L

L

D

D

R

H

A

G

N

R

-

K

-

T

-

G

-

R

-

L

-

M

-

F

-

G

L

V

G

G

R

V

I

I

V

V

I

I

A

G

-

V

-

M

-

L

-

S

-

I

F

F

A
x
Q

L
x
Q

C

F

A

V

G

G

M

I

T
x
N

S

V

M

V

Y

L

Y
|
Y

M

Y

A

A

A

-

L

-

Y

-

P

P

T

E

F

V

F

F

L

K

T

T

K

L

S

G

L

A

K

S

V

T

D

D

P

I

T

A

T

L

V

L

N

Q

T

T

V

I

L

V

L

G

A

V

T

I

A
x
N

L

L

C

T

V

F

P

T

V

V

F

-

P

-

L

L

A

A

G

I

W

M

A

T

C

V

D

D

R

K

F

F

G
|
G

R
|
R

K

K

P

P

V

L

L

Q

L

I

V

I

G

G

F

A

L

L

T

G

S

M

A

A

L

I

L

G

A

M

F

F

R133 (= R122) mutation R->C,H,L: Abolishes xylose transport.
E153 (= E142) mutation to A: Abolishes xylose transport.
R160 (= R149) mutation to A: Abolishes xylose transport.
Q168 (≠ T157) binding ; mutation to A: Abolishes xylose transport.
Q288 (≠ A246) mutation to A: Abolishes xylose transport.
QQ 288:289 (≠ AL 246:247) binding
Q289 (≠ L247) mutation to A: Strongly decreases xylose transport.
N294 (≠ T252) binding ; mutation to A: Abolishes xylose transport.
Y298 (= Y256) mutation to A: Abolishes xylose transport.
N325 (≠ A286) mutation to A: No effect on xylose transport.
G340 (= G303) mutation to A: Abolishes xylose transport.
R341 (= R304) mutation R->A,W: Abolishes xylose transport.

Sites not aligning to the query:

24 F→A: Decreases xylose transport.
83 G→A: Abolishes xylose transport.
392 binding ; W→A: Abolishes xylose transport.
397 E→A: Abolishes xylose transport.
404 R→A: Strongly decreases xylose transport.
415 binding
416 W→A: Strongly decreases xylose transport.

A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
30% identity, 29% coverage: 75:235/546 of query aligns to 63:212/446 of A0A0H2VG78

query
sites
A0A0H2VG78

G

G

R

P

I

L

G

A

D

D

M

K

I

L

G

G

R

R

K

R

R

R

T

L

F

V

M

M

-

L

I

I

T

A

I

I

C

V

M

F

M

I

G

-

A

-

T

-

I

-

G

-

V

I

G

G

C

A

L

L

P

I

T

L

Y

A

A

A

S

S

A

-

G

-

I

-

V

-

S

T

P

N

I

L

A

A

L

L

C

I

L

I

-

G

R
|
R

I

L

L

I

Q
x
I

G

G

F

L

S

A

A

V

G

G

E

S

Y

M

T

S

G

T

A

V

L

P

T

V

Y

Y

V

L

A

S

E
|
E

Y

M

S

A

P

P

G

T

R

E

R

Y

R

R

G

G

L

S

N

L

M

G

S

S

W

L

T

N

T
x
Q

A

L

S

M

S

I

T

T

V

I

G

G

L

I

L

L

L

A

S

A

F

Y

L

L

V

V

I

N

L

Y

A

A

S

F

R

A

T

D

I

I

A

E

G

G

D

-

S

-

F

-

D

-

W

W

G

R

W

W

R

M

L

L

P

G

F

L

-

A

L

V

A

V

A

P

S

S

V

V

M

I

L

L

V

V

S

G

L

I

L

Y

L

F

R

-

V

-

R

-

M

M

D

P

E

E

S

S

P

P

A

R

F

W

-

L

-

E

-

N

R

R

K

N

L

E

R

E

S

A

A

A

N

R

K

Q

L

V

S

M

K

K

A

I

P

T

L

Y

R

D

D

D

A

S

L

E

L

I

D

D

R

K

R102 (= R122) mutation to A: Loss of transport activity.
I105 (≠ Q125) mutation to S: Affects symport activity. May function as an uniporter.
E122 (= E142) mutation to A: Loss of transport activity.
Q137 (≠ T157) mutation to A: Loss of transport activity.

Sites not aligning to the query:

22 D→N: Affects symport activity. May function as an uniporter.
250 Q→A: Loss of transport activity.
251 Q→A: Loss of transport activity.
256 N→A: Loss of transport activity.
357 W→A: Loss of transport activity.

O08966 Solute carrier family 22 member 1; Organic cation transporter 1; mOCT1 from Mus musculus (Mouse) (see paper)
29% identity, 29% coverage: 69:226/546 of query aligns to 162:299/556 of O08966

query
sites
O08966

L

L

G

G

A

S

L

L

V

V

F

V

G

G

R

Y

I

I

G

A

D

D

M

R

I

F

G

G

R

R

K

K

R

L

T

C

F

L

M

L

I

V

T

T

I

T

C

L

M

V

M

T

G

S

G

L

A

S

T

G

I

V

G

L

V

T

G

A

C

V

L

A

P

P

T

D

Y

Y

A

T

S

S

A

-

G

-

I

-

V

-

S

-

P

-

I

-

A

-

L

M

L

L

C

L

L

F

R

R

I

L

L

L

Q

Q

G

G

F

M

S

V

A

S

G

K

G

G

E

S

Y

W

T

V

G

S

A

G

L

Y

T

T

Y

L

V

I

A

T

E

E

Y

F

-

V

S

G

P

S

G

G

R

Y

R

R

G

T

L

T

N

A

M

I

S

L

W

Y

T

Q

T

V

A

A

S

-

S

F

T

T

V

V

G

G

L

L

L

V

L

-

S

-

F

-

L

-

V

-

I

-

L

-

A

-

S

-

R

-

T

G

I

L

A

A

G

G

D

V

S

A

F

Y

D

A

D

I

W

P

G

D

W

W

R

R

L

W

P

L

F

Q

L

L

A

A

A

V

S

S

V

L

M

P

L

T

L

F

V

L

S

F

L

L

L

Y

R

Y

V

W

R

F

M

V

D

P

E

E

S

S

P

P

A

-

F

-

R

R

K

W

L

L

R

L

S

S

A

Q

N

K

K

R

L

T

S

T

K

Q

A

A

Sites not aligning to the query:

32 L→F: Increased trospium uptake. Increased trospium affinity. No change in fenoterol uptake.
36 Y→C: Decreased fenoterol uptake. Decreased fenoterol affinity. No change in trospium uptake. No change in terbutaline affinity.

Query Sequence

>RR42_RS24545 FitnessBrowser__Cup4G11:RR42_RS24545
MAHGPDATSPMSRAIWAASIGTIFEWYEFALYGALASVLADKFFAGVDPSTAFIFALLTF
AVGFMMRPLGALVFGRIGDMIGRKRTFMITICMMGGATIGVGCLPTYASAGIVSPIALLC
LRILQGFSAGGEYTGALTYVAEYSPGRRRGLNMSWTTASSTVGLLLSFLVILASRTIAGD
SFDDWGWRLPFLAASVMLLVSLLLRVRMDESPAFRKLRSANKLSKAPLRDALLDRANLGR
IVIAFALCAGMTSMYYMAALYPTFFLTKSLKVDPTTVNTVVLLATALCVPVFPLAGWACD
RFGRKPVLLVGFLTSALLAFPVFKGLVIYANHALAAAQAKAPISIEVDRAACSLMFNPLG
NRKFASSCDLVKQALANAGASYQIIDAAPGTTARIRIGDDVLDAYDAGGMSADQAKAQSS
QLSQALHAALEKYSYPGKSDPKAFNVTVVLLLLALFFASSILTVMTIGPALVEMFPTRIR
YTSMGVPYNLATGWVGGLLPTFVFAIAAQTGNSLCGLWYPVGWTILSLVALCFFRETRDI
DIAADA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory