SitesBLAST

P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
47% identity, 97% coverage: 5:396/403 of query aligns to 59:459/466 of P87228

query
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P87228

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S87 (≠ A33) modified: Phosphoserine
S258 (≠ T204) modified: Phosphoserine

1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
38% identity, 64% coverage: 49:307/403 of query aligns to 47:303/304 of 1wwkA

query
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active site: S96 (≠ N98), R230 (= R230), D254 (= D254), E259 (= E259), H278 (= H282)
binding nicotinamide-adenine-dinucleotide: V100 (= V102), G146 (= G148), F147 (≠ Y149), G148 (= G150), R149 (≠ N151), I150 (= I152), Y168 (= Y170), D169 (= D171), P170 (≠ V172), Y171 (≠ Q173), H200 (= H200), V201 (= V201), P202 (= P202), T207 (= T207), T228 (≠ A228), S229 (= S229), R230 (= R230), D254 (= D254), H278 (= H282), G280 (= G284), A281 (≠ G285)

O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
34% identity, 67% coverage: 49:316/403 of query aligns to 53:317/533 of O43175

query
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O43175

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T78 (≠ I74) binding
R135 (≠ K131) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
RI 155:156 (≠ NI 151:152) binding
D175 (= D171) binding
T207 (≠ V201) binding
CAR 234:236 (≠ ASR 228:230) binding
D260 (= D254) binding
V261 (= V255) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
HLGA 283:286 (≠ HIGG 282:285) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987

6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
34% identity, 64% coverage: 49:307/403 of query aligns to 49:304/305 of 6plfA

query
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6plfA

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R

K

binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: G148 (= G148), G150 (= G150), Y170 (= Y170), D171 (= D171), P172 (vs. gap), I173 (vs. gap), I174 (≠ V172), L189 (= L187), H202 (= H200), T203 (≠ V201), P204 (= P202), S208 (≠ Q206), T209 (= T207), L212 (≠ M210)

6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
34% identity, 64% coverage: 49:307/403 of query aligns to 48:303/303 of 6plgA

query
sites
6plgA

I

V

R

R

S

S

A

A

T

T

H

K

L

V

G

T

A

A

A

D

E

V

I

I

E

N

A

A

R

E

H

K

L

L

V

Q

A

V

I

V

G

G

C

R

F

A

C

G

I

T

G

G

T

V

S

D

Q

N

V

V

D

D

L

L

D

E

A

A

A

T

R

R

A

K

G

G

M

I

P

L

V

V

F

M

N

N

A

T

P

P

F

N

S

G

N

N

T

S

R

L

S

S

V

A

A

A

E

E

L

L

V

T

I

C

A

G

E

M

A

I

I

M

L

C

L

L

L

A

R

R

R

Q

V

I

P

P

E

Q

K

A

N

T

A

A

L

S

A

M

H

K

A

D

G

G

K

K

W

W

A

E

K

R

G

K

A

K

S

F

G

M

S

G

F

T

E

E

A

L

R

N

G

G

K

K

T

T

I

L

A

G

I

I

I

L

G

G

Y
x
L

G
|
G

N
x
R

I
|
I

G

G

S

R

Q

E

V

V

G

A

V

T

L

R

A

M

E

Q

A

S

M

F

G

G

M

M

R

K

V

T

V

I

Y

G

Y
|
Y

D
|
D

-
x
P

-
x
I

V
x
I

Q

S

A

P

K

E

L

V

S

S

L

A

G

S

S

F

A

G

R

V

A

Q

R

L

S

P

L

L

A

E

D

E

A

I

V

W

S

P

Q

L

A

C

D

D

I

F

V

I

T

T

L

V

H
|
H

V
x
T

P
|
P

A

L

T
x
L

A

P

Q
x
S

T

T

R

T

K

G

M

L

I

L

D

N

A

D

D

N

V

T

L

F

R

A

Q

Q

F

C

R

K

G

G

A

V

I

R

L

V

I

V

N

N

A

C

S
x
A

R
|
R

G

G

T

G

V

I

V

V

D

D

I

E

E

G

A

A

L

L

R

L

A

R

A

A

C

L

D

Q

D

S

N

G

H

Q

I

C

A

A

G

G

L

A

A

A

I

L

D

D

V

V

F

F

P

T

E

E

P

P

K

P

T

R

N

-

A

-

D

D

R

R

F

-

T

-

S

-

P

A

L

L

Q

V

G

D

V

H

P

E

N

N

A

V

I

I

L

S

T

C

P

P

H

H

I

L

G

G

G

A

S

S

T

T

E

K

E

E

A

A

Q

Q

E

S

N

R

I

C

G

G

T

E

E

E

V

I

A

A

T

V

K

Q

L

F

I

V

A

D

F

M

L

V

R

K

binding (2S)-(4-{3-[(4,5-dichloro-1-methyl-1H-indole-2-carbonyl)amino]oxetan-3-yl}phenyl)(pyridin-3-yl)acetic acid: L148 (≠ Y149), G149 (= G150), R150 (≠ N151), I151 (= I152), Y169 (= Y170), D170 (= D171), P171 (vs. gap), I172 (vs. gap), I173 (≠ V172), H201 (= H200), T202 (≠ V201), P203 (= P202), L205 (≠ T204), S207 (≠ Q206), A230 (≠ S229), R231 (= R230)

7dkmA Phgdh covalently linked to oridonin (see paper)
34% identity, 64% coverage: 49:307/403 of query aligns to 49:304/306 of 7dkmA

query
sites
7dkmA

I

V

R

R

S

S

A

A

T

T

H

K

L

V

G

T

A

A

A

D

E

V

I

I

E

N

A

A

R

E

H

K

L

L

V

Q

A

V

I

V

G

G

C

R

F

A

C

G

I
x
T

G

G

T

V

S

D

Q

N

V

V

D

D

L

L

D

E

A

A

A

T

R

R

A

K

G

G

M

I

P

L

V

V

F

M

N

N

A

T

P

P

F

N

S

G

N
|
N

T

S

R

L

S

S

V
x
A

A

A

E

E

L

L

V

T

I

C

A

G

E

M

A

I

I

M

L

C

L

L

L

A

R

R

R

Q

V

I

P

P

E

Q

K

A

N

T

A

A

L

S

A

M

H

K

A

D

G

G

K

K

W

W

A

E

K

R

G

K

A

K

S

F

G

M

S

G

F

T

E

E

A

L

R

N

G

G

K

K

T

T

I

L

A

G

I

I

I
x
L

G
|
G

Y

L

G
|
G

N
x
R

I
|
I

G

G

S

R

Q

E

V

V

G

A

V

T

L

R

A

M

E

Q

A

S

M

F

G

G

M

M

R

K

V

T

V

I

Y

G

Y
|
Y

D
|
D

-
x
P

-
x
I

V

I

Q

S

A

P

K

E

L

V

S

S

L

A

G

S

S

F

A

G

R

V

A

Q

R

L

S

P

L

L

A

E

D

E

A

I

V

W

S

P

Q

L

A

C

D

D

I

F

V

I

T

T

L

V

H
|
H

V
x
T

P
|
P

A

L

T

L

A

P

Q
x
S

T
|
T

R

T

K

G

M

L

I

L

D

N

A

D

D

N

V

T

L

F

R

A

Q

Q

F

C

R

K

G

G

A

V

I

R

L

V

I

V

N

N

A
x
C

S
x
A

R
|
R

G

G

T

G

V

I

V

V

D

D

I

E

E

G

A

A

L

L

R

L

A

R

A

A

C

L

D

Q

D

S

N

G

H

Q

I

C

A

A

G

G

L

A

A

A

I

L

D
|
D

V

V

F

F

P

T

E

E

P

P

K

P

T

R

N

-

A

-

D

D

R

R

F

-

T

-

S

-

P

A

L

L

Q

V

G

D

V

H

P

E

N

N

A

V

I

I

L

S

T

C

P

P

H
|
H

I

L

G
|
G

G
x
A

S

S

T

T

E

K

E

E

A

A

Q

Q

E

S

N

R

I

C

G

G

T
x
E

E

E

V

I

A

A

T

V

K

Q

L

F

I

V

A

D

F

M

L

V

R

K

binding nicotinamide-adenine-dinucleotide: T74 (≠ I74), N98 (= N98), A102 (≠ V102), L147 (≠ I147), G148 (= G148), G150 (= G150), R151 (≠ N151), I152 (= I152), Y170 (= Y170), D171 (= D171), P172 (vs. gap), I173 (vs. gap), H202 (= H200), T203 (≠ V201), P204 (= P202), S208 (≠ Q206), T209 (= T207), C230 (≠ A228), A231 (≠ S229), R232 (= R230), D256 (= D254), H279 (= H282), G281 (= G284), A282 (≠ G285)
binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: E293 (≠ T296)

Sites not aligning to the query:

binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 21

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
36% identity, 62% coverage: 60:307/403 of query aligns to 62:313/334 of 5aovA

query
sites
5aovA

I

F

E

E

A

N

A

A

R

P

H

R

L

L

V

R

A

I

I

V

G

A

C

N

F
x
Y

C
x
A

I
x
V

G
|
G

T

Y

S

D

Q

N

V

I

D

D

L

V

D

E

A

E

A

A

A

T

R

R

A

R

G

G

M

I

P

Y

V

V

F

T

N

N

A

T

P

P

F

D

S

V

N
x
L

T

T

R

N

S

A

V
x
T

A

A

E

D

L

H

V

A

I

F

A

A

E

L

A

L

I

L

L

A

L

T

L

A

R

R

R

H

V

V

P

V

E

K

K

G

N

D

A

K

L

F

A

V

H

R

A

S

G

G

K

E

W

W

A

K

-

R

K

K

G

G

A

I

S

A

G

W

-

H

-

P

-

K

-

W

-

F

-

L

S

G

F

Y

E

E

A

L

R

Y

G

G

K

K

T

T

I

I

A

G

I

I

I

V

G
|
G

Y
x
F

G
|
G

N
x
R

I
|
I

G

G

S

Q

Q

A

V

I

G

A

V

R

L

R

A

A

E

K

A

G

M

F

G

N

M

M

R

R

V

I

V

L

Y

Y

Y
|
Y

-
x
S

-
x
R

-
x
T

-

R

D
x
K

V

S

Q

Q

A

A

K

E

L

K

S

E

L

L

G

G

S

A

A

E

R

-

A

-

A

Y

R

R

S

P

L

L

A

E

D

E

A

V

V

L

S

K

Q

E

A

S

D

D

I

F

V

V

T

I

L

L

H
x
A

V
|
V

P
|
P

A

L

T

T

A

K

Q

E

T
|
T

R

M

K

Y

M

M

I

I

D

N

A

E

D

E

V

R

L

L

R

K

Q

L

F

M

R

K

R

P

G

T

A

A

I

I

L

L

I

V

N

N

A
x
I

S
x
A

R
|
R

G

G

T

K

V

V

D

D

I

T

E

K

A

A

L

L

R

I

A

K

A

A

C

L

D

K

D

E

N

G

H

W

I

I

A

A

G

G

L

A

A

G

I

L

D
|
D

V

V

F

F

P

E

E

E

E
|
E

P

P

K

Y

T

Y

N

N

A

E

D

E

R

L

F

F

T

S

S

-

P

-

L

-

Q

-

G

-

V

L

P

D

N

N

A

V

I

V

L

L

T

T

P

P

H
|
H

I

I

G
|
G

G

S

S

A

T

T

E

F

E

E

A

A

Q

R

E

E

N

A

I

M

G

A

T

E

E

L

V

V

A

A

T

R

K

N

L

L

I

I

A

A

F

F

L

K

R

R

active site: L100 (≠ N98), R241 (= R230), D265 (= D254), E270 (= E259), H288 (= H282)
binding glyoxylic acid: Y74 (≠ F72), A75 (≠ C73), A75 (≠ C73), V76 (≠ I74), G77 (= G75), R241 (= R230), H288 (= H282)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ I74), G77 (= G75), L100 (≠ N98), T104 (≠ V102), G157 (= G148), F158 (≠ Y149), G159 (= G150), R160 (≠ N151), I161 (= I152), Y179 (= Y170), S180 (vs. gap), R181 (vs. gap), T182 (vs. gap), K184 (≠ D171), A211 (≠ H200), V212 (= V201), P213 (= P202), T218 (= T207), I239 (≠ A228), A240 (≠ S229), R241 (= R230), D265 (= D254), H288 (= H282), G290 (= G284)

Sites not aligning to the query:

binding glyoxylic acid: 52, 53, 53

7ewhA Crystal structure of human phgdh in complex with homoharringtonine
35% identity, 63% coverage: 49:303/403 of query aligns to 48:299/302 of 7ewhA

query
sites
7ewhA

I

V

R

R

S

S

A

A

T

T

H

K

L

V

G

T

A

A

A

D

E

V

I

I

E

N

A

A

R

E

H

K

L

L

V

Q

A

V

I

V

G

G

C

R

F

A

C

G

I

T

G

G

T

V

S

D

Q

N

V

V

D

D

L

L

D

E

A

A

A

T

R

R

A

K

G

G

M

I

P

L

V

V

F

M

N

N

A

T

P

P

F

N

S

G

N

N

T

S

R

L

S

S

V

A

A

A

E

E

L

L

V

T

I

C

A

G

E

M

A

I

I

M

L

C

L

L

L

A

R

R

R

Q

V

I

P

P

E

Q

K

A

N

T

A

A

L

S

A

M

H

K

A

D

G

G

K

K

W

W

A

E

K

R

G

K

A

K

S

F

G

M

S

G

F

T

E

E

A

L

R

N

G

G

K

K

T

T

I

L

A

G

I
|
I

I
x
L

G
|
G

Y
x
L

G
|
G

N
x
R

I
|
I

G
|
G

S

R

Q

E

V

V

G

A

V

T

L

R

A

M

E

Q

A

S

M

F

G

G

M

M

R

K

V

T

V

I

Y

G

Y

Y

D
|
D

-
x
P

-
x
I

V

I

Q

S

A

P

K

E

L

V

S

S

L

A

G

S

S

F

A

G

R

V

A

Q

R

L

S

P

L

L

A

E

D

E

A

I

V

W

S

P

Q

L

A

C

D

D

I

F

V

I

T

T

L
x
V

H
|
H

V
x
T

P
|
P

A

L

T

L

A

P

Q

S

T

T

R

T

K

G

M

L

I

L

D

N

A

D

D

N

V

T

L

F

R

A

Q

Q

F

C

R

K

G

G

A

V

I

R

L

V

I

V

N

N

A

C

S

A

R

R

G

G

T

G

V

I

V

V

D

D

I

E

E

G

A

A

L

L

R

L

A

R

A

A

C

L

D

Q

D

S

N

G

H

Q

I

C

A

A

G

G

L

A

A

A

I

L

D

D

V

V

F

F

P

T

E

E

P

P

K

P

T

R

N

-

A

-

D

D

R

R

F

-

T

-

S

-

P

A

L

L

Q

V

G

D

V

H

P

E

N

N

A

V

I

I

L

S

T

C

P

P

H

H

I

L

G

G

G

A

S

S

T

T

E

K

E

E

A

A

Q

Q

E

S

N

R

I

C

G

G

T

E

E

E

V

I

A

A

T

V

K

Q

L

F

I

V

binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: I145 (= I146), L146 (≠ I147), G147 (= G148), L148 (≠ Y149), G149 (= G150), R150 (≠ N151), I151 (= I152), G152 (= G153), D170 (= D171), P171 (vs. gap), I172 (vs. gap), V200 (≠ L199), H201 (= H200), T202 (≠ V201), P203 (= P202)

6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
35% identity, 63% coverage: 49:303/403 of query aligns to 48:299/302 of 6rihA

query
sites
6rihA

I

V

R

R

S

S

A

A

T

T

H

K

L

V

G

T

A

A

A

D

E

V

I

I

E

N

A

A

R

E

H

K

L

L

V

Q

A

V

I

V

G

G

C

R

F

A

C

G

I

T

G

G

T

V

S

D

Q

N

V

V

D

D

L

L

D

E

A

A

A

T

R

R

A

K

G

G

M

I

P

L

V

V