SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS32635 FitnessBrowser__Cup4G11:RR42_RS32635 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

Q01468 2-hydroxymuconate tautomerase; 4-oxalocrotonate tautomerase; 4-OT; EC 5.3.2.6 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
46% identity, 100% coverage: 1:63/63 of query aligns to 1:63/63 of Q01468

query
sites
Q01468

M
|
M

P
|
P

I

I

M

A

Q

Q

V

I

F

H

L

I

I

L

E

E

G

G

R

R

T

S

E

D

E

E

Q

Q

K

K

A

E

G

T

L

L

I

I

R

R

A

E

L

V

T

S

D

E

A

A

A

I

V

S

E

R

S

S

I

L

G

D

A

A

P

P

V

L

E

T

S

S

V

V

R

R

V

V

M

I

I

I

T

T

D

E

V

M

P

A

N

K

T

G

Q

H

F

F

G

G

I

I

A

G

G

G

K

E

T

L

A

A

K

S

E

K

L

V

G

R

R

R

M1 (= M1) modified: Initiator methionine, Removed
P2 (= P2) active site, Proton acceptor; via imino nitrogen

5tigA Crystal structure of 4-oxalocrotonate tautomerase inactivated by brhpd (see paper)
45% identity, 98% coverage: 2:63/63 of query aligns to 1:62/62 of 5tigA

query
sites
5tigA

P
|
P

I

I

M

A

Q

Q

V

I

F

H

L

I

I

L

E

E

G

G

R

R

T

S

E

D

E

E

Q

Q

K

K

A

E

G

T

L

L

I

I

R

R

A

E

L

V

T

S

D

E

A

A

A

I

V

S

E

R

S

S

I

L

G

D

A

A

P

P

V

L

E

T

S

S

V

V

R
|
R

V

V

M

I

I

I

T

T

D

E

V

M

P

A

N

K

T

G

Q

H

F
|
F

G

G

I

I

A

G

G

G

K

E

T

L

A

A

K

S

E

K

L

V

G
x
R

R

R

active site: P1 (= P2), R39 (= R40), F50 (= F51), R61 (≠ G62)
binding (3E)-5-hydroxy-2-oxopent-3-enoic acid: F50 (= F51), R61 (≠ G62)

1bjpA Crystal structure of 4-oxalocrotonate tautomerase inactivated by 2- oxo-3-pentynoate at 2.4 angstroms resolution (see paper)
45% identity, 98% coverage: 2:63/63 of query aligns to 1:62/62 of 1bjpA

query
sites
1bjpA

P
|
P

I
|
I

M

A

Q

Q

V

I

F

H

L

I

I

L

E

E

G

G

R
|
R

T

S

E

D

E

E

Q

Q

K

K

A

E

G

T

L

L

I

I

R

R

A

E

L

V

T

S

D

E

A

A

A

I

V

S

E

R

S

S

I

L

G

D

A

A

P

P

V

L

E

T

S

S

V

V

R
|
R

V

V

M

I

I

I

T

T

D

E

V

M

P

A

N

K

T

G

Q

H

F
|
F

G

G

I

I

A

G

G

G

K

E

T

L

A

A

K

S

E

K

L

V

G
x
R

R

R

active site: P1 (= P2), R11 (= R12), R39 (= R40), F50 (= F51)
binding 2-oxo-3-pentenoic acid: P1 (= P2), I2 (= I3), F50 (= F51), R61 (≠ G62)

6bgnA Crystal structure of 4-oxalocrotonate tautomerase after incubation with 5-fluoro-2-hydroxy-2,4-pentadienoate (see paper)
46% identity, 94% coverage: 2:60/63 of query aligns to 1:59/59 of 6bgnA

query
sites
6bgnA

P
|
P

I

I

M

A

Q

Q

V

I

F

H

L

I

I

L

E

E

G

G

R

R

T

S

E

D

E

E

Q

Q

K

K

A

E

G

T

L

L

I

I

R

R

A

E

L

V

T

S

D

E

A

A

A

I

V

S

E

R

S

S

I

L

G

D

A

A

P

P

V

L

E

T

S
|
S

V

V

R
|
R

V

V

M

I

I

I

T

T

D

E

V

M

P

A

N

K

T

G

Q

H

F
|
F

G

G

I

I

A

G

G

G

K

E

T

L

A

A

K

S

E

K

active site: P1 (= P2), R39 (= R40), F50 (= F51)
binding 5-fluoranyl-2-oxidanylidene-pentanoic acid: P1 (= P2), S37 (= S38)

6ghwC Substituting the prolines of 4-oxalocrotonate tautomerase with non- canonical analogue (2s)-3,4-dehydroproline (see paper)
47% identity, 90% coverage: 2:58/63 of query aligns to 1:57/57 of 6ghwC

query
sites
6ghwC

P
|
P

I

I

M

A

Q

Q

V

I

F

H

L

I

I

L

E

E

G

G

R

R

T

S

E

D

E

E

Q

Q

K

K

A

E

G

T

L

L

I

I

R

R

A

E

L

V

T

S

D

E

A

A

A

I

V

S

E
x
R

S

S

I

L

G
x
D

A

A

P

P

V

L

E

T

S

S

V

V

R
|
R

V

V

M

I

I

I

T

T

D

E

V

M

P

A

N

K

T

G

Q

H

F
|
F

G

G

I

I

A

G

G

G

K

E

T

L

A

A

active site: P1 (= P2), R39 (= R40), F50 (= F51)
binding calcium ion: R29 (≠ E30), D32 (≠ G33)

5cloM Crystal structure of a 4-oxalocrotonate tautomerase mutant in complex with nitrostyrene at 2.3 angstrom (see paper)
46% identity, 90% coverage: 2:58/63 of query aligns to 1:57/57 of 5cloM

query
sites
5cloM

P
|
P

I

I

M

A

Q

Q

V

I

F

H

L

I

I

L

E

E

G

G

R

R

T

S

E

D

E

E

Q

Q

K

K

A

E

G

T

L

L

I

I

R

R

A

E

L

V

T

S

D

E

A

A

A

I

V

S

E

R

S

S

I

L

G

D

A

A

P

P

V

L

E

T

S
|
S

V

V

R
|
R

V

V

M

I

I

I

T

T

D

E

V

Y

P

A

N

K

T

G

Q

H

F
x
A

G

G

I

I

A

G

G

G

K

E

T

L

A

A

active site: P1 (= P2), R39 (= R40), A50 (≠ F51)
binding trans beta nitrostyrene: P1 (= P2), S37 (= S38)

P70994 2-hydroxymuconate tautomerase; (2Z,4E)-2-hydroxyhexa-2,4-dienedioate keto-enol isomerase; 4-oxalocrotonate tautomerase; 4-OT; EC 5.3.2.6 from Bacillus subtilis (strain 168) (see paper)
39% identity, 89% coverage: 1:56/63 of query aligns to 1:56/62 of P70994

query
sites
P70994

M

M

P
|
P

I

Y

M

V

Q

T

V

V

F

K

L

M

I

L

E

E

G

G

R
|
R

T

T

E

D

E

E

Q

Q

K

K

A

R

G

N

L

L

I

V

R

E

A

K

L

V

T

T

D

E

A

A

A

V

V

K

E

E

S

T

I

T

G

G

A

A

P

S

V

E

E

E

S

K

V

I

R

V

V

V

M

F

I

I

T

E

D

E

V

M

P

R

N

K

T

D

Q

H

F

Y

G

A

I

V

A

A

G

G

K

K

P2 (= P2) active site, Proton acceptor; via imino nitrogen; mutation to A: Absence of tautomerase activity using 2-hydroxymuconate. For 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate, a strong decrease of catalytic efficiency and a 2-fold decrease of affinity is observed.
R12 (= R12) mutation to A: Absence of tautomerase activity using 2-hydroxymuconate. For 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate, a strong decrease of catalytic efficiency and a 2-fold decrease of affinity is observed.

2opaA Ywhb binary complex with 2-fluoro-p-hydroxycinnamate
38% identity, 87% coverage: 2:56/63 of query aligns to 1:55/61 of 2opaA

query
sites
2opaA

P

P

I

Y

M

V

Q

T

V

V

F

K

L
x
M

I

L

E

E

G

G

R
|
R

T

T

E

D

E

E

Q

Q

K

K

A

R

G

N

L

L

I

V

R

E

A

K

L

V

T

T

D

E

A

A

A

V

V

K

E

E

S

T

I

T

G

G

A

A

P

S

V

E

E

E

S

K

V

I

R
x
V

V

V

M

F

I

I

T

E

D

E

V

M

P

R

N

K

T

D

Q

H

F
x
Y

G

A

I

V

A

A

G

G

K

K

active site: V39 (≠ R40), Y50 (≠ F51)
binding 2-fluoro-3-(4-hydroxyphenyl)-2e-propeneoate: M7 (≠ L8), R11 (= R12), Y50 (≠ F51)

Sites not aligning to the query:

active site: 61

Query Sequence

>RR42_RS32635 FitnessBrowser__Cup4G11:RR42_RS32635
MPIMQVFLIEGRTEEQKAGLIRALTDAAVESIGAPVESVRVMITDVPNTQFGIAGKTAKE
LGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory