SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS33940 FitnessBrowser__Cup4G11:RR42_RS33940 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

5bokA Ferredoxin component of 3-nitrotoluene dioxygenase from diaphorobacter sp. Strain ds2
56% identity, 98% coverage: 3:103/103 of query aligns to 1:101/102 of 5bokA

query
sites
5bokA

K

E

Q

N

W

W

T

I

Q

D

V

A

I

A

P

A

L

R

V

D

A

E

I

V

P

P

K

E

D

G

D

D

V

V

V

I

A

G

V

I

N

N

A

I

L

V

G

G

K

K

E

E

I

I

A

A

L

L

Y

Y

S

E

V

V

G

A

D

G

A

E

I

I

Y

Y

A

A

T

T

D

D

N

N

A

T

C
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C

S

T

H
|
H

G
|
G

N

A

A
|
A

R

R

L

M

C

S

D

D

G

G

F

F

L

L

D

E

G

G

H

R

E

E

I

I

E

E

C
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C

P

P

L
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L

H
|
H

Q

Q

G

G

K

R

F

F

D

D

V

V

R

C

T

T

G

G

R

K

A

A

M

L

C

C

E

T

P
|
P

L

L

T

T

S

Q

D

D

I

I

Q

K

A

T

Y

Y

S

P

V

V

K

K

V

I

E

E

N

N

G

M

A

R

V

V

F

M

V

L

E

K

L

L

binding fe2/s2 (inorganic) cluster: C43 (= C45), H45 (= H47), G46 (= G48), A48 (= A50), C62 (= C64), L64 (= L66), H65 (= H67), P80 (= P82)

P0A185 Naphthalene 1,2-dioxygenase system, ferredoxin component from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
53% identity, 100% coverage: 1:103/103 of query aligns to 1:103/104 of P0A185

query
sites
P0A185

M
|
M

S

T

K

V

Q

K

W

W

T

I

Q

E

V

A

I

V

P

A

L

L

V

S

A

D

I

I

P

L

K

E

D

G

D

D

V

V

V

L

A

G

V

V

N

T

A

V

L

E

G

G

K

K

E

E

I

L

A

A

L

L

Y

Y

S

E

V

V

G

E

D

G

A

E

I

I

Y

Y

A

A

T

T

D

D

N

N

A

L

C
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C

S

T

H
|
H

G

G

N

S

A

A

R

R

L

M

C

S

D

D

G

G

F

Y

L

L

D

E

G

G

H

R

E

E

I

I

E

E

C
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C

P

P

L

L

H
|
H

Q

Q

G

G

K

R

F

F

D

D

V

V

R

C

T

T

G

G

R

K

A

A

M

L

C

C

E

A

P

P

L

V

T

T

S

Q

D

N

I

I

Q

K

A

T

Y

Y

S

P

V

V

K

K

V

I

E

E

N

N

G

L

A

R

V

V

F

M

V

I

E

D

L

L

M1 (= M1) modified: Initiator methionine, Removed
C45 (= C45) binding
H47 (= H47) binding
C64 (= C64) binding
H67 (= H67) binding

2qpzA Naphthalene 1,2-dioxygenase rieske ferredoxin (see paper)
54% identity, 97% coverage: 4:103/103 of query aligns to 3:102/103 of 2qpzA

query
sites
2qpzA

Q

K

W

W

T

I

Q

E

V

A

I

V

P

A

L

L

V

S

A

D

I

I

P

L

K

E

D

G

D

D

V

V

V

L

A

G

V

V

N

T

A

V

L

E

G

G

K

K

E

E

I

L

A

A

L

L

Y

Y

S

E

V

V

G

E

D

G

A

E

I

I

Y

Y

A

A

T

T

D

D

N

N

A

L

C
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C

S

T

H
|
H

G
|
G

N

S

A

A

R

R

L

M

C

S

D

D

G

G

F

Y

L

L

D

E

G

G

H

R

E

E

I

I

E

E

C
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C

P

P

L
|
L

H
|
H

Q

Q

G
|
G

K

R

F

F

D

D

V

V

R

C

T

T

G

G

R

K

A

A

M

L

C

C

E

A

P
|
P

L

V

T

T

S

Q

D

N

I

I

Q

K

A

T

Y

Y

S

P

V

V

K

K

V

I

E

E

N

N

G

L

A

R

V

V

F

M

V

I

E

D

L

L

binding fe2/s2 (inorganic) cluster: C44 (= C45), H46 (= H47), G47 (= G48), C63 (= C64), L65 (= L66), H66 (= H67), G68 (= G69), P81 (= P82)

Q8GI16 Ferredoxin CarAc; Carbazole 1,9a-dioxygenase, ferredoxin component; CARDO from Pseudomonas resinovorans (see 2 papers)
39% identity, 98% coverage: 1:101/103 of query aligns to 1:102/107 of Q8GI16

query
sites
Q8GI16

M

M

S

N

K

Q

Q

I

W

W

T

L

Q

K

V

V

I

C

P

A

L

A

V

S

A

D

I

M

P

Q

K

P

D

G

D

T

V

I

V

R

A

R

V

V

N

N

A

R

L

V

G

G

K

A

-

A

E

P

I

L

A

A

L

V

Y

Y

S

R

V

V

G

G

D

D

A

Q

I

F

Y

Y

A

A

T

T

D

E

N

D

A

T

C
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C

S

T

H
|
H

G

G

N

I

A

A

R

S

L

L

C

S

D

E

G

G

F

T

L

L

D

D

G

G

H

D

E

V

I

I

E

E

C
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C

P

P

L

F

H
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H

Q

G

G

G

K

A

F

F

D

N

V

V

R

C

T

T

G

G

R

M

A

P

M

A

C

S

E

S

P

P

L

C

T

T

S

V

D

P

I

L

Q

G

A

V

Y

F

S

E

V

V

K

E

V

V

E

K

N

E

G

G

A

E

V

V

F

Y

V

V

C46 (= C45) binding
H48 (= H47) binding
C65 (= C64) binding
H68 (= H67) binding

2i7fA Sphingomonas yanoikuyae b1 ferredoxin (see paper)
46% identity, 72% coverage: 29:102/103 of query aligns to 27:100/102 of 2i7fA

query
sites
2i7fA

I

L

A

A

L

V

Y

Y

S

N

V

V

G

D

D

G

A

E

I

V

Y

F

A

V

T

T

D

D

N

N

A

L

C
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C

S

T

H
|
H

G
|
G

N

N

A

A

R

M

L

L

C

T

D

D

G

G

F

Y

L

Q

D

D

G

G

H

T

E

I

I

I

E

E

C
|
C

P

P

L
x
F

H
|
H

Q

G

G

G

K

S

F

F

D

D

V

I

R

A

T

T

G

G

R

A

A

A

M

K

C

A

E

F

P

P

L

C

T

Q

S

I

D

P

I

I

Q

K

A

T

Y

Y

S

P

V

V

K

T

V

I

E

E

N

D

G

G

A

W

V

V

F

C

V

I

E

D

binding fe2/s2 (inorganic) cluster: C43 (= C45), H45 (= H47), G46 (= G48), C62 (= C64), F64 (≠ L66), H65 (= H67)

4nbbE Carbazole- and oxygen-bound oxygenase with ile262 replaced by val and ferredoxin complex of carbazole 1,9a-dioxygenase (see paper)
40% identity, 94% coverage: 5:101/103 of query aligns to 4:101/114 of 4nbbE

query
sites
4nbbE

W

W

T

L

Q

K

V

V

I

C

P

A

L

A

V

S

A

D

I

M

P

Q

K

P

D

G

D

T

V

I

V

R

A

R

V

V

N

N

A

R

L

V

G

G

K

A

-

A

E

P

I

L

A

A

L

V

Y

Y

S

R

V

V

G

G

D

D

A

Q

I

F

Y

Y

A

A

T

T

D

E

N

D

A

T

C
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C

S

T

H
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H

G
|
G

N

I

A

A

R

S

L

L

C

S

D

E

G

G

F

T

L

L

D

D

G

G

H

D

E

V

I

I

E

E

C
|
C

P

P

L
x
F

H
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H

Q

G

G
|
G

K

A

F

F

D

N

V

V

R

C

T

T

G

G

R

M

A

P

M

A

C

S

E

S

P

P

L

C

T

T

S

V

D

P

I

L

Q

G

A

V

Y

F

S

E

V

V

K

E

V

V

E

K

N

E

G

G

A

E

V

V

F

Y

V

V

binding fe2/s2 (inorganic) cluster: C45 (= C45), H47 (= H47), G48 (= G48), C64 (= C64), F66 (≠ L66), H67 (= H67), G69 (= G69)

1fqtA Crystal structure of the rieske-type ferredoxin associated with biphenyl dioxygenase (see paper)
32% identity, 97% coverage: 4:103/103 of query aligns to 2:102/109 of 1fqtA

query
sites
1fqtA

Q

K

W

F

T

T

Q

R

V

V

I

C

P

D

L

R

V

R

A

D

I

V

P

P

K

E

D

G

D

E

V

A

V

L

A

K

V

V

N

E

A

S

L

G

G

G

K

T

E

S

I

V

A

A

L

I

Y

F

S

N

V

V

G

D

D

G

A

E

I

L

Y

F

A

A

T

T

D

Q

N

D

A

R

C
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C

S

T

H
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H

G
|
G

N

D

A

W

R

S

L

L

C

S

D

D

G

G

-

G

F

Y

L

L

D

E

G

G

H

D

E

V

I

V

E

E

C
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C

P

S

L

L

H
|
H

Q

M

G
|
G

K

K

F

F

D

C

V

V

R

R

T

T

G

G

R

K

A

V

M

K

C

S

E

P

P

P

L
x
P

T

C

S

E

D

A

I

L

Q

K

A

I

Y

F

S

P

V

I

K

R

V

I

E

E

N

D

G

N

A

D

V

V

F

L

V

V

E

D

L

F

binding fe2/s2 (inorganic) cluster: C43 (= C45), H45 (= H47), G46 (= G48), C63 (= C64), H66 (= H67), G68 (= G69), P82 (≠ L83)

2e4pA Crystal structure of bpha3 (oxidized form) (see paper)
31% identity, 96% coverage: 5:103/103 of query aligns to 2:101/108 of 2e4pA

query
sites
2e4pA

W

F

T

T

Q

K

V
x
A

I

C

P

S

L

V

V

D

A
x
E

I

V

P

P

K

P

D

G

D

E

V

A

V

L

A

Q

V

V

N

S

A

H

L

D

G

A

K

Q

E

K

I

V

A

A

L

I

Y

F

S

N

V

V

G

D

D

G

A

E

I

F

Y

F

A

A

T

T

D

Q

N

D

A

Q

C
|
C

S

T

H
|
H

G
|
G

N

E

A

W

R

S

L

L

C

S

D

E

G

G

-

G

F

Y

L

L

D

D

G

G

H

D

E

V

I

V

E

E

C
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C

P

S

L

L

H
|
H

Q

M

G
|
G

K

K

F

F

D

C

V

V

R

R

T

T

G

G

R

K

A

V

M

K

C

S

E

P

P

P

L

P

T

C

S

E

D

P

I

L

Q

K

A

V

Y

Y

S

P

V

I

K

R

V

I

E

E

N

G

G

R

A

D

V

V

F

L

V

V

E

D

L

F

binding fe2/s2 (inorganic) cluster: C42 (= C45), H44 (= H47), G45 (= G48), C62 (= C64), H65 (= H67), G67 (= G69)
binding alpha-D-glucopyranose: A5 (≠ V8), E10 (≠ A13)

3dqyA Crystal structure of toluene 2,3-dioxygenase ferredoxin (see paper)
33% identity, 96% coverage: 5:103/103 of query aligns to 2:100/106 of 3dqyA

query
sites
3dqyA

W

W

T

T

Q

Y

V

I

I

L

P

R

L

Q

V

G

A

D

I

L

P

P

K

P

D

G

D

E

V

M

V

Q

A

R

V

Y

N

E

A

G

L

G

G

P

K

E

E

P

I

V

A

M

L

V

Y

C

S

N

V

V

G

D

D

G

A

E

I

F

Y

F

A

A

T

V

D

Q

N

D

A

T

C
|
C

S

T

H
|
H

G
|
G

N

D

A

W

R

A

L

L

C

S

D

D

G

G

F

Y

L

L

D

D

G

G

H

D

E

I

I

V

E

E

C
|
C

P

T

L

L

H
|
H

Q

F

G
|
G

K

K

F

F

D

C

V

V

R

R

T

T

G

G

R

K

A

V

M

K

C

A

E

L

P

P

L

A

T

C

S

K

D

P

I

I

Q

K

A

V

Y

F

S

P

V

I

K

K

V

V

E

E

N

G

G

D

A

E

V

V

F

H

V

V

E

D

L

L

binding fe2/s2 (inorganic) cluster: C42 (= C45), H44 (= H47), G45 (= G48), C61 (= C64), H64 (= H67), G66 (= G69)

3gceA Ferredoxin of carbazole 1,9a-dioxygenase from nocardioides aromaticivorans ic177 (see paper)
41% identity, 79% coverage: 21:101/103 of query aligns to 19:101/104 of 3gceA

query
sites
3gceA

A

A

V

F

N

D

A

V

L

D

G

G

K

D

E

E

I

V

A

M

L

V

Y

V

S

R

V

D

G

G

D

D

A

S

I

V

Y

Y

A

A

T

I

D

S

N

N

A

L

C
|
C

S

S

H
|
H

G
x
A

N

E

A

A

R

Y

L

L

C

D

D

M

G

G

F

V

L

F

-

H

-

A

D

E

G

S

H

L

E

E

I

I

E

E

C
|
C

P

P

L

L

H
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H

Q

V

G
|
G

K

R

F

F

D

D

V

V

R

R

T

T

G

G

R

A

A

P

M

T

C

A

E

L

P
|
P

L

C

T

V

S

L

D

P

I

V

Q

R

A

A

Y

Y

S

D

V

V

K

V

V

V

E

D

N

G

G

T

A

E

V

I

F

L

V

V

binding fe2/s2 (inorganic) cluster: C43 (= C45), H45 (= H47), A46 (≠ G48), C64 (= C64), H67 (= H67), G69 (= G69), P82 (= P82)

6icoA Pseudomonas putida cbb5 ndma with theophylline (see paper)
30% identity, 52% coverage: 20:73/103 of query aligns to 31:84/344 of 6icoA

query
sites
6icoA

V

L

A

A

V

V

N

K

A

L

L

L

G

N

K

E

E

Q

I

L

A

V

L

V

Y

A

S

K

V

L

G

G

D

D

A

E

I

Y

Y

V

A

A

T

M

D

R

N

D

A

R

C
|
C

S

A

H
|
H

G
x
R

N

S

A

A

R

K

L

L

C

S

D

L

G

G

F

T

L

V

D

S

G

G

H

N

E

R

I

L

E

Q

C
|
C

P

P

L
x
Y

H
|
H

Q

G

G
x
W

K

Q

F

Y

D

D

V

T

binding fe2/s2 (inorganic) cluster: C56 (= C45), H58 (= H47), R59 (≠ G48), C75 (= C64), Y77 (≠ L66), H78 (= H67), W80 (≠ G69)

Sites not aligning to the query:

binding cobalt (ii) ion: 161, 167, 172, 283
binding theophylline: 162, 213, 242, 276

6icnA Pseudomonas putida cbb5 ndma with caffeine (see paper)
30% identity, 52% coverage: 20:73/103 of query aligns to 31:84/344 of 6icnA

query
sites
6icnA

V

L

A

A

V

V

N

K

A

L

L

L

G

N

K

E

E

Q

I

L

A

V

L

V

Y

A

S

K

V

L

G

G

D

D

A

E

I

Y

Y

V

A

A

T

M

D

R

N

D

A

R

C
|
C

S

A

H
|
H

G
x
R

N

S

A

A

R

K

L

L

C

S

D

L

G

G

F

T

L

V

D

S

G

G

H

N

E

R

I

L

E

Q

C
|
C

P

P

L
x
Y

H
|
H

Q

G

G
x
W

K

Q

F

Y

D

D

V

T

binding fe2/s2 (inorganic) cluster: C56 (= C45), H58 (= H47), R59 (≠ G48), C75 (= C64), Y77 (≠ L66), H78 (= H67), W80 (≠ G69)

Sites not aligning to the query:

binding caffeine: 162, 168, 213, 242, 276, 279, 280
binding cobalt (ii) ion: 161, 167, 172, 283

6icqA Pseudomonas putida cbb5 ndma ql mutant with theobromine (see paper)
30% identity, 52% coverage: 20:73/103 of query aligns to 31:84/334 of 6icqA

query
sites
6icqA

V

L

A

A

V

V

N

K

A

L

L

L

G

N

K

E

E

Q

I

L

A

V

L

V

Y

A

S

K

V

L

G

G

D

D

A

E

I

Y

Y

V

A

A

T

M

D

R

N

D

A

R

C
|
C

S

A

H
|
H

G
x
R

N

S

A

A

R

K

L

L

C

S

D

L

G

G

F

T

L

V

D

S

G

G

H

N

E

R

I

L

E

Q

C
|
C

P

P

L
x
Y

H
|
H

Q

G

G
x
W

K

Q

F

Y

D

D

V

T

binding fe2/s2 (inorganic) cluster: C56 (= C45), H58 (= H47), R59 (≠ G48), C75 (= C64), Y77 (≠ L66), H78 (= H67), W80 (≠ G69)

Sites not aligning to the query:

binding theobromine: 232, 266
binding fe (iii) ion: 161, 167, 172, 273

6ickA Pseudomonas putida cbb5 ndma (see paper)
30% identity, 52% coverage: 20:73/103 of query aligns to 31:84/330 of 6ickA

query
sites
6ickA

V

L

A

A

V

V

N

K

A

L

L

L

G

N

K

E

E

Q

I

L

A

V

L

V

Y

A

S

K

V

L

G

G

D

D

A

E

I

Y

Y

V

A

A

T

M

D

R

N

D

A

R

C
|
C

S

A

H
|
H

G
x
R

N

S

A

A

R

K

L

L

C

S

D

L

G

G

F

T

L

V

D

S

G

G

H

N

E

R

I

L

E

Q

C
|
C

P

P

L
x
Y

H
|
H

Q

G

G
x
W

K

Q

F

Y

D

D

V

T

binding fe2/s2 (inorganic) cluster: C56 (= C45), H58 (= H47), R59 (≠ G48), C75 (= C64), Y77 (≠ L66), H78 (= H67), W80 (≠ G69)

Sites not aligning to the query:

binding fe (iii) ion: 167, 172, 269

Query Sequence

>RR42_RS33940 FitnessBrowser__Cup4G11:RR42_RS33940
MSKQWTQVIPLVAIPKDDVVAVNALGKEIALYSVGDAIYATDNACSHGNARLCDGFLDGH
EIECPLHQGKFDVRTGRAMCEPLTSDIQAYSVKVENGAVFVEL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory