SitesBLAST

6wo1A Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit (see paper)
25% identity, 44% coverage: 1:224/512 of query aligns to 9:234/551 of 6wo1A

query
sites
6wo1A

M

L

N

S

G

G

A

G

E

Q

S

I

L

F

V

Q

K

E

T

M

L

M

L

L

A

R

C

H

G

D

V

V

D

K

T

Q

C

V

F

F

A

G

N
x
Y

P

P

G
|
G

T
x
G

S
x
A

E
x
I

M

L

H

P

F

V

V

F

A

D

A

A

L

I

D

Y

R

N

I

S

P

P

G

H

M

F

R

E

C

F

V

V

L

L

G

P

L

R

F

H

E
|
E

G

Q

V

G

V

A

T

G

G

H

A

M

A

A

D

E

G

G

Y

Y

A

A

R

R

M

V

A

S

D

G

K

K

P

P

A

G

A

V

T

V

L

L

L

V

H
x
T

C

S

G

G

P

P

G

G

L

A

A

T

N

N

G

V

L

I

A

T

N

P

L

M

H

Q

N

D

A

A

R

L

R

S

A
x
D

Q

G

T

V

P

P

V

M

I

V

N

V

I

F

V

C

G

G

D

Q

Q

V

A

A

T

T

Y

N

H

L

R

I

P

G

F

S

D

D

A

A

P
x
F

L
x
Q

T
x
E

A

A

D

D

T

V

E

V

G

G

W

I

A

S

R

R

P

S

V

C

S

T

V

K

W

W

T

N

R

V

T

M

A

V

T

K

D

D

A

I

A

A

S

E

V

L

G

P

A

R

D

R

A

I

A

N

T

E

A

A

V

F

Q

K

A

I

A

A

C

T

A

T

A

G

P
x
R

G

P

G

G

V

P

A

V

S

L

L

V

I

D

L

L

P

P

S
x
K

D

D

V

V

C

T

W

-

D

-

A

-

G

-

K

-

V

A

G

A

V

I

P

L

L

R

A

T

P

P

L

I

P

P

V

A

P

P

-

L

K

P

V

G

S

D

P

A

D

D

A

L

V

I

Q

T

Q

E

A

A

R

Q

L

M

L

I

R

N

S

K

G

A

Q

K

P

R

T

P

L

-

I

I

V

I

L

F

A

A

G
|
G

T

N

A
x
G

L

V

R

L

E
x
S

V

S

P

P

-

E

-

G

-

P

-

K

-

L

-

K

-

E

L

L

A

S

D

D

A

K

H

G

R

R

I

I

A

P

A

V

A

T

T

T

active site: Y30 (≠ N22), G32 (= G24), G33 (≠ T25), A34 (≠ S26), I35 (≠ E27), E56 (= E48), T79 (≠ H71), F118 (≠ P110), Q119 (≠ L111), E120 (≠ T112), K168 (≠ S160)
binding flavin-adenine dinucleotide: D97 (≠ A89), R158 (≠ P150), G208 (= G206), G210 (≠ A208), S213 (≠ E211)

Sites not aligning to the query:

active site: 255, 282, 398, 424, 426, 451, 478
binding 2-methylpyrimidin-4-amine: 424, 425, 426
binding diphosphate: 398, 399, 400, 401, 450, 451, 452, 453
binding flavin-adenine dinucleotide: 235, 236, 237, 253, 254, 255, 275, 276, 277, 279, 281, 282, 308, 309, 327, 328, 402, 421, 422
binding magnesium ion: 451, 478

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
27% identity, 42% coverage: 296:512/512 of query aligns to 419:635/664 of P09114

query
sites
P09114

V

I

L

L

A

E

R

S

P

K

E

E

Q

G

D

K

L

L

A

K

E

L

A

D

L

F

A

S

R

A

L

W

A

R

D

Q

E

E

L

L

G

T

A

V

P

Q

S

K

V

V

S

K

V

Y

P

P

A

L

S

N

V

F

A

K

P

T

R

F

G

G

I

-

A

-

K

-

G

D

A

A

V

I

T

P

S

P

E

Q

A

Y

V

A

A

I

Q

Q

T

V

L

L

T

D

A

E

L

L

L

T

P

N

E

G

Q

S

A

A

I

I

V

I

-

S

-

T

-

G

V

V

E

G

E

Q

S

H

V

Q

S

M

F

W

G

A

R

A

A

Q

F

Y

Y

Y

P

K

G

-

T

-

V

-

H

Y

A

R

A

K

P

P

H

R

D

Q

W

W

L

L

Q

T

L

S

T

G

G

G

-

L

G

G

A

A

I

M

G

G

D

F

G

G

L

L

P

P

L

A

A

A

T

I

G

G

A

A

V

V

A

G

A

R

P

P

G

D

R

E

R

V

V

V

A

D

L

I

Q

D

A

G

D

D

G

G

S

S

G

F

M

I

Y

M

T

N

L

V

Q

Q

S

E

L

L

W

A

T

T

M

I

A

K

R

V

E

E

K

N

L

L

D

P

V

V

T

K

V

I

V

M

I

L

L

L

A

N

N

N

R

Q

K

H

Y

L

A

G

I

M

L

V

G

Q

-
x
W

E

E

L

D

A

R

G

F

V

Y

G

K

A

A

N

N

P

R

G

A

K

H

T

T

A

Y

L

L

D

-

M

-

L

-

D

-

I

-

G

G

N

N

P

P

D

S

L

N

D

E

-

A

-

E

-

I

-

F

-

P

-

N

W

M

V

L

Q

K

L

F

A

A

N

E

G

A

M

C

G

G

V

V

E

P

A

A

R

R

A

V

E

T

D

H

M

R

E

D

R

D

F

L

A

R

E

A

L

A

F

I

G

Q

M

K

A

M

N

L

G

D

R

T

R

P

G

G

P

P

F

Y

V

L

I

L

E

D

L

V

V

I

I

V

W568 (vs. gap) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

Sites not aligning to the query:

191 P→A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
25% identity, 41% coverage: 2:210/512 of query aligns to 13:227/582 of 7tzzA

query
sites
7tzzA

N

K

G

G

A

A

E

D

S

I

L

L

V

V

K

E

T

A

L

L

L

E

A

R

C

Q

G

G

V

V

D

E

T

T

C

V

F

F

A

A

N

Y

P

P

G

G

T

G

S
x
A

E

S

M

M

H

E

F

I

V

H

A

Q

A

A

L

L

D

T

R

R

I

S

P

S

G

S

M

I

R

R

C

N

V

V

L

L

G

P

L

R

F

H

E

E

G

Q

V

G

T

V

G

F

A

A

D

E

G

G

Y

Y

A

A

R

R

M

S

A

S

D

G

K

K

P

P

A

G

A

I

T

C

L

I

L

A

H
x
T

C
x
S

G

G

P

P

G

G

L

A

A

T

N

N

G

L

L

V

A

S

N

G

L

L

H

A

N

D

A

A

R

L

A

D

Q

S

T

V

P

P

V

L

I

V

N

A

I

I

V

T

G

G

D

Q

Q

V

A

T

T

R

Y

R

H

M

R

I

P

G

F

T

D

D

A

A

P

F

L
x
Q

T

E

A

T

D

P

T

I

E

V

G

E

W

V

A

T

R

R

P

S

V

I

S

T

V

K

W

H

T

N

R

Y

T

L

A

V

T

M

D

D

A

V

A

E

S

D

V

I

G

P

A

R

D

I

A

I

A

E

T

E

A

A

V

F

Q

F

A

L

A

A

C

T

A

S

A

G

P
x
R

G

P

G

G

V

P

A

V

S

L

L

V

I

D

L

V

P

P

S

K

D

D

V

I

-

Q

-

L

-

A

-

I

-

P

C

N

W

W

D

E

A

Q

G

A

G

M

K

R

V

L

G

P

V

G

P

Y

L

M

A

S

P

R

L

M

P

P

V

K

P

P

K

P

V

-

S

E

P

D

D

S

A

H

V

L

Q

E

Q

Q

A

I

A

V

R

R

L

L

L

I

R

S

S

E

G

S

Q

K

P

K

T

P

L

V

I

L

V

Y

L

V

A
x
G

G
|
G

T
x
G

A

C

L

L

R

N

binding flavin-adenine dinucleotide: R161 (≠ P150), G222 (≠ A205), G223 (= G206), G224 (≠ T207)
binding magnesium ion: A37 (≠ S26), T82 (≠ H71), S83 (≠ C72), Q122 (≠ L111)

Sites not aligning to the query:

binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 266, 292, 489, 568
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: 400, 401, 402, 403, 426, 428, 452, 453, 454, 455, 483, 484, 485, 486
binding flavin-adenine dinucleotide: 246, 247, 248, 263, 264, 266, 267, 286, 288, 293, 310, 311, 329, 330, 405, 423
binding magnesium ion: 381, 453, 458, 461, 480, 482, 533

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
38% identity, 19% coverage: 2:98/512 of query aligns to 98:194/670 of P17597

query
sites
P17597

N

K

G

G

A

A

E

D

S

I

L

L

V

V

K

E

T

A

L

L

L

E

A

R

C

Q

G

G

V

V

D

E

T

T

C

V

F

F

A

A

N

Y

P

P

G

G

T

G

S
x
A

E

S

M
|
M

H

E

F

I

V

H

A

Q

A

A

L

L

D

T

R

R

I

S

P

S

G

S

M

I

R

R

C

N

V

V

L

L

G

P

L

R

F

H

E
|
E

G

Q

V

G

T

V

G

F

A

A

D

E

G

G

Y

Y

A

A

R

R

M

S

A

S

D

G

K

K

P

P

A

G

A

I

T

C

L

I

L

A

H

T

C

S

G

G

P

P

G

G

L

A

A

T

N

N

G

L

L

V

A

S

N

G

L

L

H

A

N

D

A

A

R

L

A

D

Q
x
S

T

V

P

P

V

L

I

V

N

A

I

I

V

T

G

G

A122 (≠ S26) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (= M28) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E48) binding
S186 (≠ Q90) binding

Sites not aligning to the query:

197 P→S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
199 mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
207 binding
220 binding
246 binding ; binding
256 binding
308 binding
331:332 binding
340 modified: Cysteine sulfinic acid (-SO2H)
349:352 binding
371:375 binding
376:377 binding
395:396 binding
414:415 binding
487:488 binding
508:509 binding
511:513 binding
538 binding
538:540 binding
565 binding
565:570 binding
567 binding
574 binding ; W→L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; W→S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
653 binding ; S→A: No effect on catalytic activity or sensitivity to herbicides.; S→F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; S→N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; S→T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
38% identity, 19% coverage: 2:98/512 of query aligns to 13:109/583 of 5k3sA

query
sites
5k3sA

N

K

G

G

A

A

E

D

S

I

L

L

V

V

K

E

T

A

L

L

L

E

A

R

C

Q

G

G

V

V

D

E

T

T

C

V

F

F

A

A

N
x
Y

P

P

G
|
G

T
x
G

S
x
A

E
x
S

M

M

H

E

F

I

V

H

A

Q

A

A

L

L

D

T

R

R

I

S

P

S

G

S

M

I

R

R

C

N

V

V

L

L

G

P

L

R

F

H

E
|
E

G

Q

V

G

T

V

G

F

A

A

D

E

G

G

Y

Y

A

A

R

R

M

S

A

S

D

G

K

K

P

P

A

G

A

I

T

C

L

I

L

A

H
x
T

C

S

G

G

P

P

G

G

L

A

A

T

N

N

G

L

L

V

A

S

N

G

L

L

H

A

N

D

A

A

R

L

A

D

Q

S

T

V

P

P

V

L

I

V

N

A

I

I

V

T

G

G

active site: Y33 (≠ N22), G35 (= G24), G36 (≠ T25), A37 (≠ S26), S38 (≠ E27), E59 (= E48), T82 (≠ H71)

Sites not aligning to the query:

active site: 121, 122, 123, 171, 266, 293, 400, 426, 428, 453, 480, 482, 483, 485, 486, 489, 558
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 292, 485, 489, 569
binding flavin-adenine dinucleotide: 161, 222, 223, 224, 246, 247, 248, 264, 266, 286, 288, 290, 293, 310, 311, 329, 330, 405, 423
binding magnesium ion: 453, 480, 482
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: 400, 401, 402, 403, 426, 428, 453, 454, 455, 480, 482, 483, 484, 485, 486

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
38% identity, 19% coverage: 2:98/512 of query aligns to 13:109/582 of 8et4A

query
sites
8et4A

N

K

G

G

A

A

E

D

S

I

L

L

V

V

K

E

T

A

L

L

L

E

A

R

C

Q

G

G

V

V

D

E

T

T

C

V

F

F

A

A

N

Y

P

P

G

G

T

G

S

A

E

S

M

M

H

E

F

I

V

H

A

Q

A

A

L

L

D

T

R

R

I

S

P

S

G

S

M

I

R

R

C

N

V

V

L

L

G

P

L

R

F

H

E

E

G

Q

V

G

T

V

G

F

A

A

D

E

G

G

Y

Y

A

A

R

R

M

S

A

S

D

G

K

K

P

P

A

G

A

I

T

C

L

I

L

A

H

T

C

S

G

G

P

P

G

G

L

A

A

T

N

N

G

L

L

V

A

S

N

G

L

L

H

A

N

D

A

A

R

L

A

D

Q

S

T

V

P

P

V

L

I

V

N

A

I

I

V

T

G

G

Sites not aligning to the query:

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: 400, 401, 402, 403, 426, 428, 452, 453, 454, 455, 458, 480, 482, 483, 484, 485, 486
binding flavin-adenine dinucleotide: 161, 222, 223, 224, 246, 247, 248, 264, 266, 267, 286, 287, 288, 290, 292, 293, 310, 311, 329, 330, 405, 423
binding magnesium ion: 370, 453, 458, 461, 480, 482, 533
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: 266, 292, 485, 489, 568

Query Sequence

>RR42_RS36655 FitnessBrowser__Cup4G11:RR42_RS36655
MNGAESLVKTLLACGVDTCFANPGTSEMHFVAALDRIPGMRCVLGLFEGVVTGAADGYAR
MADKPAATLLHCGPGLANGLANLHNARRAQTPVINIVGDQATYHRPFDAPLTADTEGWAR
PVSVWTRTATDAASVGADAATAVQAACAAPGGVASLILPSDVCWDAGGKVGVPLAPLPVP
KVSPDAVQQAARLLRSGQPTLIVLAGTALREVPLADAHRIAAATDARLITPMSNARVSRG
RGRFNVDRVPYSGDVARDKLAGIRNVILVGAPPPVTFFAYPGKSPRPYPEDAEIHVLARP
EQDLAEALARLADELGAPSVSVPASVAPRGIAKGAVTSEAVAQTLTALLPEQAIVVEESV
SFGRAFYPGTVHAAPHDWLQLTGGAIGDGLPLATGAAVAAPGRRVVALQADGSGMYTLQS
LWTMAREKLDVTVVILANRKYAILLGELAGVGANPGKTALDMLDIGNPDLDWVQLANGMG
VEAARAEDMERFAELFGMANGRRGPFVIELVI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory