SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SM_b20316 FitnessBrowser__Smeli:SM_b20316 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4pz0A The crystal structure of a solute binding protein from bacillus anthracis str. Ames in complex with quorum-sensing signal autoinducer-2 (ai-2)
28% identity, 90% coverage: 34:333/333 of query aligns to 13:321/321 of 4pz0A

query
sites
4pz0A

V

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F

binding (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran: K15 (= K36), F21 (≠ W42), D96 (≠ E118), D145 (≠ T166), Q146 (≠ H167), W149 (= W170), P199 (≠ G220), D200 (≠ S221), A201 (= A222), W246 (= W267)

3c6qC Apo and ligand-bound form of a thermophilic glucose/xylose binding protein
31% identity, 71% coverage: 42:279/333 of query aligns to 14:255/305 of 3c6qC

query
sites
3c6qC

W

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F
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binding beta-D-xylopyranose: W15 (≠ F43), D89 (≠ E118), N137 (≠ T166), R141 (≠ W170), E164 (≠ N195), Y190 (≠ S221), A191 (= A222), D216 (≠ G247), Q236 (≠ W267)

Sites not aligning to the query:

binding beta-D-xylopyranose: 9

2h3hA Crystal structure of the liganded form of thermotoga maritima glucose binding protein (see paper)
31% identity, 71% coverage: 42:279/333 of query aligns to 14:255/313 of 2h3hA

query
sites
2h3hA

W

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binding beta-D-glucopyranose: D89 (≠ E118), N137 (≠ T166), R141 (≠ W170), E164 (≠ N195), Y190 (≠ S221), A191 (= A222), D216 (≠ G247), Q236 (≠ W267)

Sites not aligning to the query:

binding beta-D-glucopyranose: 9

1tjyA Crystal structure of salmonella typhimurium ai-2 receptor lsrb in complex with r-thmf (see paper)
26% identity, 92% coverage: 28:333/333 of query aligns to 3:316/316 of 1tjyA

query
sites
1tjyA

A

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F

binding (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran: K11 (= K36), F17 (≠ W42), F18 (= F43), D92 (≠ E118), D142 (vs. gap), Q143 (≠ H167), W146 (= W170), P196 (≠ G220), D197 (≠ S221), A198 (= A222), W242 (= W267)

6guqA Crystal structure of ganp, a glucose-galactose binding protein from geobacillus stearothermophilus, in complex with glucose
27% identity, 75% coverage: 41:289/333 of query aligns to 14:260/278 of 6guqA

query
sites
6guqA

N

D

W
x
Y

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E

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D

D

S

A

L

P

K

T

N

S

T

R

Q

R

V

V

D

A

I

Y

E

V

A

G

F

T

D

D

N

N

K

Y

V

Y

F

A

G

G

A

F

R

L

F

A

N

G

E

R

K

A

L

L

A

A

E

E

C

-

M

-

G

D

K

T

S

K

G

G

K

K

W

A

T

T

S

V

F

A

V

I

G

I

S

T

L

-

G

G

S

S

L

L

T

T

-

A

H
|
H

V

Q

Q

Q

W

L

A
x
R

D

V

G

R

G

G

A

F

E

E

N

D

A

A

K

V

K

R

Y

Q

P

E

E

K

M

G

E

I

L

R

V

I

S

V

E

A

K

I

N

E

E

E

S

S

F

H

N
x
I

D

T

A

R

N

V

K

Q

A

A

Y

A

E

E

K

K

A

A

R

Y

E

T

I

I

L

L

R

K

K

K

Y

H

P

P

D

D

I

V

K

N

G

A

F

F

Q

Y

G

G

G

T

S
|
S

A
|
A

I

L

D

D

V

A

I

I

G

G

I

V

G

A

R

K

A

V

V

V

E

E

E

Q

A

F

G

H

L

R

V

E

G

Q

K

K

V

T

C

Y

V

I

G

G

L

F

G
x
D

L

T

P

L

K

P

D

E

T

T

A

I

K

R

Y

Y

L

L

E

Q

S

K

G

G

A

T

V

I

Q

A

S

A

I

T

S

V

F

V

W
x
Q

D

E

P

P

K

Y

D

E

A

M

G

G

Y

Y

V

K

M

A

N

V

K

K

V

M

A

M

Q

A

L

E

V

I

I

V

E

A

G

G

K

K

E

D

I

V

binding beta-D-glucopyranose: Y15 (≠ W42), D90 (≠ E118), H138 (= H167), R142 (≠ A171), I166 (≠ N195), S192 (= S221), A193 (= A222), D218 (≠ G247), Q238 (≠ W267)

Sites not aligning to the query:

binding beta-D-glucopyranose: 9, 13

5hqjA Crystal structure of abc transporter solute binding protein b1g1h7 from burkholderia graminis c4d1m, target efi-511179, in complex with d-arabinose
28% identity, 67% coverage: 67:288/333 of query aligns to 43:266/289 of 5hqjA

query
sites
5hqjA

P

P

A

Q

K

N

A

T

D

Q

A

G

A

S

Q

T

Q

Q

L

V

R

Q

L

I

I

I

E

E

D

D

L

L

V

I

A

S

K

R

N

H

V

V

N

D

A

G

I

I

A

A

V

I

V

S

P

V

M

N

D

E

P

P

S

K

A

S

L

V

E

E

G

S

V

V

F

M

K

K

R

R

A

A

M

E

N

Q

R

S

G

G

I

I

K

K

I

V

I

L

T

T

H

Y

E
x
D

A

S

D

D

S

S

L

P

K

K

N

S

T

G

Q

R

V

S

D

M

I

Y

E

I

A

G

F

T

D

N

N

N

K

E

V

Q

F

A

G

G

A

A

R

T

F

M

N

A

E

E

K

T

L

M

A

G

E

K

C

A

M

L

G

N

K

G

S

Q

G

G

K

E

W

V

T

A

S

I

F

I

V

T

G

G

S

Q

L

L

G

G

S

A

L

V

T
x
N

H

L

V

N

Q

E

W
x
R

A

I

D

A

G

G

G

I

A

K

E

K

N

G

A

L

K

A

K

K

Y

Y

P

P

E

G

M

I

E

K

L

V

V

V

S

-

E

E

K

T

N

Q

E

G

S

T

F

D

N

D

D

D

A

L

N

A

K

R

A

G

Y

V

E

S

K

V

A

V

R

E

E

T

I

T

L

L

R

R

K

A

Y

H

P

P

D

N

I

L

K

K

G

G

F

I

Q

F

G

G

G

V

S
|
S

A
x
Q

I

V

D

G

V

G

I

P

G

A

I

V

G

A

R

K

A

V

V

L

-

N

-

T

-

R

E

E

E

F

A

G

G

A

L

M

V

K

G

G

K

K

V

L

C

E

V

V

V

L

G

A

L

F

G
x
D

L

D

P

L

K

P

D

D

T

T

A

L

K

K

Y

G

L

L

E

K

S

D

G

G

A

Y

V

I

Q

Q

S

G

I

I

S

M

F

V

W
x
Q

D

R

P

P

K

V

D

T

A

M

G

G

Y

S

-

L

-

A

V

V

M

D

N

H

K

L

V

V

A

A

Q

Q

L

-

V

-

I

I

E

Q

G

G

K

Q

E

E

binding alpha-D-arabinopyranose: D94 (≠ E118), N142 (≠ T166), R146 (≠ W170), S196 (= S221), Q197 (≠ A222), D225 (≠ G247), Q245 (≠ W267)

Sites not aligning to the query:

binding alpha-D-arabinopyranose: 14, 19

6gt9A Crystal structure of ganp, a glucose-galactose binding protein from geobacillus stearothermophilus, in complex with galactose
27% identity, 75% coverage: 41:289/333 of query aligns to 19:265/283 of 6gt9A

query
sites
6gt9A

N

D

W
x
Y

F
x
W

T

R

R

L

M

V

E

E

E

K

G

G

V

A

V

K

A

A

Y

A

G

A

K

K

D

E

N

-

P

L

A

G

V

V

S

D

T

L

S

E

Q

Y

I

I

G

G

P

P

A

R

K

Q

A

A

D

N

A

I

A

D

Q

E

Q

H

L

L

R

R

L

I

I

L

E

K

D

K

L

A

V

A

A

A

K

A

N

K

V

V

N

D

A

G

I

I

A

I

V

T

V

Q

P

G

M

L

D

T

P

E

S

A

A

E

L

F

E

V

G

P

V

V

F

I

K

N

R

E

A

I

M

T

N

D

R

K

G

N

I

I

K

P

I

V

T

T

H

I

E
x
D

A

T

D

D

S

A

L

P

K

T

N

S

T

R

Q

R

V

V

D

A

I

Y

E

V

A

G

F

T

D

D

N

N

K

Y

V

Y

F

A

G

G

A

F

R

L

F

A

N

G

E

R

K

A

L

L

A

A

E

E

C

-

M

-

G

D

K

T

S

K

G

G

K

K

W

A

T

T

S

V

F

A

V

I

G

I

S

T

L

-

G

G

S

S

L

L

T

T

-

A

H
|
H

V

Q

Q

Q

W

L

A
x
R

D

V

G

R

G

G

A

F

E

E

N

D

A

A

K

V

K

R

Y

Q

P

E

E

K

M

G

E

I

L

R

V

I

S

V

E

A

K

I

N

E

E

E

S

S

F

H

N
x
I

D

T

A

R

N

V

K

Q

A

A

Y

A

E

E

K

K

A

A

R

Y

E

T

I

I

L

L

R

K

K

K

Y

H

P

P

D

D

I

V

K

N

G

A

F

F

Q

Y

G

G

G

T

S
|
S

A
|
A

I

L

D

D

V

A

I

I

G

G

I

V

G

A

R

K

A

V

V

V

E

E

E

Q

A

F

G

H

L

R

V

E

G

Q

K

K

V

T

C

Y

V

I

G

G

L

F

G
x
D

L

T

P

L

K

P

D

E

T

T

A

I

K

R

Y

Y

L

L

E

Q

S

K

G

G

A

T

V

I

Q

A

S

A

I

T

S

V

F

V

W
x
Q

D

E

P

P

K

Y

D

E

A

M

G

G

Y

Y

V

K

M

A

N

V

K

K

V

M

A

M

Q

A

L

E

V

I

I

V

E

A

G

G

K

K

E

D

I

V

binding beta-D-galactopyranose: Y20 (≠ W42), W21 (≠ F43), D95 (≠ E118), H143 (= H167), R147 (≠ A171), I171 (≠ N195), S197 (= S221), A198 (= A222), D223 (≠ G247), Q243 (≠ W267)

Sites not aligning to the query:

binding beta-D-galactopyranose: 14, 18

3t95A Crystal structure of lsrb from yersinia pestis complexed with autoinducer-2 (see paper)
26% identity, 92% coverage: 28:333/333 of query aligns to 1:314/314 of 3t95A

query
sites
3t95A

A

A

P

E

S

R

V

I

V

A

T

F

V

I

V

P

K
|
K

V

L

T

V

G

G

E

V

N

G

W
x
F

F
|
F

T

T

R

S

M

G

E

G

E

K

G

G

V

A

V

V

A

D

Y

A

G

G

K

K

D

A

N

-

P

L

A

G

V

V

S

D

T

V

S

T

Q

Y

I

D

G

G

P

P

A

T

K

E

A

P

D

S

A

V

A

S

Q

G

Q

Q

L

V

R

Q

L

L

I

I

E

N

D

N

L

F

V

V

A

N

K

Q

N

G

V

Y

N

N

A

A

I

I

A

V

V

V

V

S

P

A

M

V

D

S

P

P

S

D

A

G

L

L

E

C

G

P

V

A

F

L

K

K

R

R

A

A

M

M

N

Q

R

R

G

G

I

V

K

K

I

I

I

L

T

T

H

W

E
x
D

A

S

D

D

S

T

L

K

K

P

N

E

T

C

Q

R

-

S

V

V

D

Y

I

I

E

N

A

Q

F

G

D

T

N

P

K

N

V

Q

F

L

G

G

A

S

R

M

F

L

N

V

E

D

K

M

L

A

A

A

E

N

C

Q

M

V

G

K

K

K

S

E

G

Q

K

A

W

K

T

V

S

A

F

F

V

F

G

Y

S

S

L

S

G

P

S

T

L

V

T

T

-
x
D

H
x
Q

V

N

Q

Q

W
|
W

A

V

D

N

G

E

G

A

A

K

E

K

N

K

A

I

K

Q

-

E

Y

H

P

P

E

G

M

W

E

E

L

I

V

V

S

T

E

T

K

Q

N

-

E

F

S

G

F

Y

N

N

D

D

A

A

N

T

K

K

A

S

Y

L

E

Q

K

T

A

A

R

E

E

G

I

I

L

L

R

K

K

A

Y

Y

P

A

D

D

I

L

K

D

G

A

F

I

Q

I

G

A

G
x
P

S
x
D

A
|
A

I

N

D

A

V

L

I

P

G

A

I

A

G

A

R

Q

A

A

V

A

E

E

E

N

A

L

G

K

L

R

V

-

G

A

K

N

V

V

C

A

V

I

V

V

G

G

L

F

G

S

L

T

P

P

K

N

D

V

T

M

A

R

K

P

Y

Y

L

V

E

E

S

R

G

G

A

T

V

V

Q

K

S

E

I

F

S

G

F

L

W
|
W

D

D

P

V

K

V

D

N

A

Q

G

G

Y

K

V

I

M

S

N

V

K

Y

V

V

A

A

Q

N

L

E

V

M

I

L

E

K

G

K

K

G

E

D

I

L

T

N

D

V

G

G

M

D

D

K

L

I

G

D

V

I

P

P

G

N

Y

I

N

G

K

V

V

V

S

E

V

V

K

S

-

P

-

N

-

R

-

V

-

Q

-

G

-

Y

-

D

-

Y

N

E

G

A

P

K

G

G

D

N

G

G

I

I

I

V

V

L

G

P

E

Q

A

R

W

-

V

V

D

I

V

F

D

T

K

K

S

E

N

N

Y

I

S

S

Q

K

Y

Y

P

D

F

F

binding (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran: K9 (= K36), F15 (≠ W42), F16 (= F43), D90 (≠ E118), D140 (vs. gap), Q141 (≠ H167), W144 (= W170), P194 (≠ G220), D195 (≠ S221), A196 (= A222), W240 (= W267)

3ejwA Crystal structure of the sinorhizobium meliloti ai-2 receptor, smlsrb (see paper)
26% identity, 91% coverage: 31:333/333 of query aligns to 4:315/315 of 3ejwA

query
sites
3ejwA

V

I

V

A

T

F

V

I

V

P

K
|
K

V

L

T

V

G

G

E

V

N

G

W
x
F

F
|
F

T

T

R

S

M

G

E

G

E

A

G

G

V

A

V

V

A

K

Y

A

G

G

K

E

D

E

N

V

P

G

A

A

V

K

S

V

T

T

S

Y

Q

D

I

-

G

G

P

P

A

T

K

E

A

P

D

S

A

V

A

S

Q

G

Q

Q

L

V

R

Q

L

F

I

I

E

N

D

N

L

F

V

V

A

N

K

Q

N

G

V

Y

N

N

A

A

I

L

A

I

V

V

V

S

P

S

M

V

D

S

P

P

S

D

A

G

L

L

E

C

G

P

V

A

F

L

K

K

R

R

A

A

M

M

N

E

R

R

G

G

I

V

K

L

I

V

I

M

T

T

H

W

E
x
D

A

S

D

D

S

V

L

N

K

P

N

D

T

C

Q

R

-

S

V

Y

D

Y

I

I

E

N

A

Q

F

G

D

T

N

P

K

E

V

Q

F

L

G

G

A

G

R

L

F

L

N

V

E

D

K

M

L

A

A

A

E

E

C

G

M

V

G

K

K

K

S

E

G

K

K

A

W

K

T

V

S

A

F

F

V

F

G

Y

S

S

L

S

G

P

S

T

L

V

T

T

-
x
D

-
x
Q

H

N

V

A

Q
x
W

W

A

A

E

D

A

G

A

G

K

A

A

E

K

N

I

A

A

K

K

K

E

Y

H

P

P

E

G

M

W

E

E

L

I

V

V

S

T

E

T

K

Q

N

-

E

Y

S

G

F

Y

N

N

D

D

A

A

N

Q

K

K

A

S

Y

L

E

Q

K

T

A

A

R

E

E

S

I

I

L

L

R

Q

K

T

Y

Y

P

P

D

D

I

L

K

D

G

A

F

I

Q

I

G

A

G
x
P

S
x
D

A
|
A

I

N

D

A

V

L

I

P

G

A

I

A

G

A

R

Q

A

A

V

A

E

E

E

N

A

L

G

K

L

R

V

A

G

E

K

G

V

V

C

T

V

I

V

V

G

G

L

F

G

S

L

T

P

P

K

N

D

V

T

M

A

R

K

P

Y

Y

L

I

E

E

S

R

G

G

A

T

V

I

Q

Q

S

R

I

F

S

G

F

L

W
|
W

D

D

P

V

K

T

D

Q

A

Q

G

G

Y

K

V

I

M

S

N

V

K

F

V

V

A

A

Q

D

L

H

V

V

I

L

E

K

G

N

K

G

E

P

I

M

T

K

D

V

G

G

M

E

D

K

L

L

G

E

V

I

P

P

G

G

Y

V

N

G

K

T

V

V

S

E

V

V

K

S

-

A

-

N

-

K

-

V

-

Q

-

G

-

Y

-

D

-

Y

N

E

G

A

P

D

G

G

D

N

G

G

I

I

V

L

G

P

E

E

A

R

W

T

V

V

D

-

V

F

D

T

K

K

S

E

N

N

Y

I

S

G

Q

N

Y

F

P

D

F

F

binding (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran: K9 (= K36), F15 (≠ W42), F16 (= F43), D90 (≠ E118), D140 (vs. gap), Q141 (vs. gap), W144 (≠ Q169), P194 (≠ G220), D195 (≠ S221), A196 (= A222), W241 (= W267)

5dteB Crystal structure of an abc transporter periplasmic solute binding protein (ipr025997) from actinobacillus succinogenes 130z(asuc_0081, target efi-511065) with bound d-allose
28% identity, 73% coverage: 27:270/333 of query aligns to 1:249/287 of 5dteB

query
sites
5dteB

D

D

A

K

P

P

S

Q

V

I

V

A

T

L

V

L

V

M

K
|
K

V

T

T

L

G

S

E
x
N

N

E

W
x
Y

F

F

T

I

R

S

M

M

E

R

E

Q

G

G

V

A

V

E

A

E

Y

T

G

A

K

K

D

Q

N

K

P

D

A

I

V

D

S

L

T

I

S

V

Q

Q

I

V

G

A

P

E

A

K

K

E

A

D

D

S

A

T

A

E

Q

Q

Q

L

L

V

R

G

L

L

I

V

E

E

D

N

L

M

V

I

A

A

K

K

N

K

V

V

N

D

A

A

I

I

A

I

V

V

V

T

P

P

M

N

D

D

P

S

S

I

A

A

L

F

E

I

G

P

V

A

F

F

K

Q

R

K

A

A

M

E

N

K

R

A

G

G

I

I

K

P

I

I

-

D

-

L

-
x
D

-

V

-

R

-

L

-

D

-

A

-

K

T

A

H

A

E

E

A

A

D

A

S

G

L

L

K

K

N

F

T

N

Q

Y

V

V

D

G

I

V

E

D

A

N

F

F

D

N

N

G

K

G

V

Y

F

L

G

E

A

A

R

K

F

-

N

-

E

-

K

N

L

L

A

A

E

E

C

A

M

I

G

G

K

K

S

K

G

G

K

N

W

V

T

A

S

I

F

L

V

E

G

G

S

I

L

P

G

G

S

V

L

D

T
x
N

H

G

V

E

Q

Q

W
x
R

A

K

D

G

G

G

G

A

A

L

E

K

N

A

A

F

K

A

K

E

Y

Y

P

P

E

D

M

I

E

K

L

I

V

V

S

A

E

S

K

Q

N

S

E

A

S

N

F
x
W

N

-

D

E

A

T

N

E

K

Q

A

A

Y

L

E

N

K

V

A

T

R

T

E

N

I

I

L

L

R

T

K

A

Y

N

P

P

D

N

I

I

K

N

G

G

F

I

Q

F

G

A

S

N

A

D

I

N

D

M

V

A

I

I

G

G

I

A

G

V

R

T

A

A

V

V

E

E

E

N

A

A

G

G

L

L

V

A

G

G

K

K

V

V

C

L

V

V

V

S

G

G

L

Y

-
x
D

G

G

L

I

P

P

K

L

D

-

T

A

A

I

K

E

Y

Y

L

V

E

K

S

Q

G

G

A

K

V

M

Q

Q

S

N

I

T

S

I

F

D

W
x
Q

D

L

P

P

K

K

binding beta-D-allopyranose: K10 (= K36), N14 (≠ E40), Y16 (≠ W42), D92 (vs. gap), N146 (≠ T166), R150 (≠ W170), W174 (≠ F194), D226 (vs. gap), Q246 (≠ W267)

1rpjA Crystal structure of d-allose binding protein from escherichia coli (see paper)
30% identity, 62% coverage: 67:273/333 of query aligns to 40:253/288 of 1rpjA

query
sites
1rpjA

P

P

A

S

K
x
E

A

G

D

D

A

F

A

Q

Q

S

Q

Q

L

L

R

Q

L

L

I

F

E

E

D

D

L

L

V

S

A

N

K

K

N

N

V

Y

N

K

A

G

I

I

A

A

V

F

V

A

P

P

M

L

D

S

P

S

S

V

A

N

L

L

E

V

G

M

V

P

F

V

K

A

R

R

A

A

M

W

N

K

R

K

G

G

I

I

K

Y

I

L

I

V

T

N

H

L

E
x
D

-

E

-

K

-

I

-

D

A

M

D

D

S

N

L

L

K

K

N

K

T

A

Q

G

V

G

D

N

I

V

E

E

A

A

F

F

-

V

-

T

D

D

N

N

K

V

V

A

F

V

G

G

A

A

R

K

F

G

N

A

E

S

K

F

L

I

A

I

E

D

C

K

M

L

G

G

K

A

S

E

G

G

K

G

W

E

T

V

S

A

F

I

V

I

-

E

G

G

S

K

L

A

G

G

S

N

L

A

T
x
S

H

G

V

E

Q

A

W
x
R

A

R

D

N

G

G

G

A

A

T

E

E

N

A

A

F

K

K

Y

A

P

S

E

Q

M

I

E

K

L

L

V

V

S

A

E

S

K

Q

N

P

E

A

S

D

F
x
W

N

-

D

D

A

R

N

I

K

K

A

A

Y

L

E

D

K

V

A

A

R

T

E

N

I

V

L

L

R

Q

K

R

Y

N

P

P

D

N

I

I

K

K

G

A

F

I

Q

Y

G

C

G

A

S
x
N

A

D

I

T

D

M

V

A

I

M

G

G

I

V

G

A

R

Q

A

A

V

V

E

A

E

N

A

A

G

G

L

K

V

T

G

G

K

K

V

V

C

L

V

V

G

G

L

T

-
x
D

G

G

L

I

P

P

K

-

D

E

T

A

A

R

K

K

Y

M

L

V

E

E

S

A

G

G

A

Q

V

M

Q

T

S

A

I

T

S

V

F

A

W
x
Q

D

N

P

P

K

A

D

D

A

I

G

G

binding beta-D-allopyranose: E42 (≠ K69), D91 (≠ E118), S147 (≠ T166), R151 (≠ W170), W175 (≠ F194), N201 (≠ S221), D227 (vs. gap), Q247 (≠ W267)

Sites not aligning to the query:

binding beta-D-allopyranose: 9, 13, 15

1gudA Hinge-bending motion of d-allose binding protein from escherichia coli: three open conformations (see paper)
30% identity, 62% coverage: 67:273/333 of query aligns to 40:253/288 of 1gudA

query
sites
1gudA

P

P

A

S

K

E

A

G

D

D

A

F

A

Q

Q

S

Q

Q

L

L

R

Q

L

L

I

F

E

E

D

D

L

L

V

S

A

N

K

K

N

N

V

Y

N

K

A

G

I

I

A

A

V

F

V

A

P

P

M

L

D

S

P

S

S

V

A

N

L

L

E

V

G

M

V

P

F

V

K

A

R

R

A

A

M

W

N

K

R

K

G

G

I

I

K

Y

I

L

I

V

T

N

H

L

E

D

-

E

-

K

-

I

-

D

A

M

D

D

S

N

L

L

K

K

N

K

T

A

Q

G

V

G

D

N

I

V

E

E

A

A

F

F

-

V

-

T

D

D

N

N

K

V

V

A

F

V

G

G

A

A

R

K

F

G

N

A

E

S

K

F

L

I

A

I

E

D

C

K

M

L

G

G

K

A

S

E

G

G

K

G

W

E

T

V

S

A

F

I

V

I

-

E

G

G

S

K

L

A

G

G

S

N

L

A

T

S

H

G

V

E

Q

A

W

R

A

R

D

N

G

G

G

A

A

T

E

E

N

A

A

F

K

K

Y

A

P

S

E

Q

M

I

E

K

L

L

V

V

S

A

E

S

K

Q

N

P

E

A

S

D

F

W

N

-

D

D

A

R

N

I

K

K

A

A

Y

L

E

D

K

V

A

A

R

T

E

N

I

V

L

L

R

Q

K

R

Y

N

P

P

D

N

I

I

K

K

G

A

F

I

Q

Y

G

C

G

A

S

N

A

D

I

T

D

M

V

A

I

M

G

G

I

V

G

A

R

Q

A

A

V

V

E

A

E

N

A

A

G

G

L

K

V

T

G

G

K

K

V

V

C

L

V

V

G

G

L

T

-

D

G

G

L

I

P

P

K

-

D

E

T

A

A

R

K

K

Y

M

L

V

E

E

S

A

G

G

A

Q

V

M

Q

T

S

A

I

T

S

V

F

A

W

Q

D

N

P

P

K

A

D

D

A

I

G

G

Sites not aligning to the query:

binding zinc ion: 3, 35

8wlbA X-ray structure of enterobacter cloacae allose-binding protein in complex with d-psicose (see paper)
29% identity, 62% coverage: 67:273/333 of query aligns to 40:253/288 of 8wlbA

query
sites
8wlbA

P

P

A

S

K

E

A

G

D

D

A

F

A

Q

Q

S

Q

Q

L

L

R

Q

L

L

I

F

E

E

D

D

L

L

V

S

A

N

K

K

N

K

V

Y

N

K

A

G

I

I

A

A

V

F

V

A

P

P

M

L

D

S

P

S

S

V

A

N

L

L

E

V

G

M

V

P

F

V

K

A

R

R

A

A

M

W

N

Q

R

K

G

G

I

L

K

Y

I

L

I

V

T

N

H

L

E
x
D

-
x
E

-

K

-

I

-

D

A

M

D

D

S

N

L

L

K

K

N

K

T

A

Q

G

V

G

D

N

I

V

E

E

A

G

F

F

-

V

-

T

D

D

N

N

K

V

V

A

F

V

G

G

A

A

R

K

F

G

N

A

E

D

K

F

L

I

A

I

E

N

C

K

M

L

G

G

K

A

S

E

G

G

K

G

W

E

T

V

S

A

F

I

V

I

-

E

G

G

S

K

L

A

G

G

S

N

L

A

T
x
S

H

G

V

E

Q

A

W
x
R

A

R

D

N

G

G

G

A

A

T

E

E

N

A

A

F

K

K

Y

A

P

N

E

Q

M

I

E

K

L

L

V

V

S

A

E

S

K

Q

N

P

E

A

S

D

F

W

N

-

D

D

A

R

N

I

K

K

A

A

Y

L

E

D

K

V

A

A

R

T

E

N

I

V

L

L

R

Q

K

R

Y

N

P

P

D

N

I

L

K

K

G

A

F

F

Q

Y

G

C

G

A

S
x
N

A

D

I

T

D

M

V

A

I

M

G

G

I

V

G

A

R

Q

A

A

V

V

E

A

E

N

A

A

G

G

L

K

V

I

G

G

K

K

V

V

C

L

V

V

G

G

L

T

-
x
D

G

G

L

I

P

P

K

-

D

E

T

A

A

R

K

K

Y

M

L

V

E

E

S

A

G

G

A

Q

V

M

Q

T

S

A

I

T

S

V

F

A

W
x
Q

D

N

P

P

K

A

D

D

A

I

G

G

binding alpha-D-psicopyranose: D91 (≠ E118), E92 (vs. gap), S147 (≠ T166), R151 (≠ W170), N201 (≠ S221), D227 (vs. gap), Q247 (≠ W267)

Sites not aligning to the query:

binding alpha-D-psicopyranose: 9, 13, 15, 16

8wl9A X-ray structure of enterobacter cloacae allose-binding protein in complex with d-ribose (see paper)
29% identity, 62% coverage: 67:273/333 of query aligns to 40:253/288 of 8wl9A

query
sites
8wl9A

P

P

A

S

K

E

A

G

D

D

A

F

A

Q

Q

S

Q

Q

L

L

R

Q

L

L

I

F

E

E

D

D

L

L

V

S

A

N

K

K

N

K

V

Y

N

K

A

G

I

I

A

A

V

F

V

A

P

P

M

L

D

S

P

S

S

V

A

N

L

L

E

V

G

M

V

P

F

V

K

A

R

R

A

A

M

W

N

Q

R

K

G

G

I

L

K

Y

I

L

I

V

T

N

H

L

E
x
D

-

E

-

K

-

I

-

D

A

M

D

D

S

N

L

L

K

K

N

K

T

A

Q

G

V

G

D

N

I

V

E

E

A

G

F

F

-

V

-

T

D

D

N

N

K

V

V

A

F

V

G

G

A

A

R

K

F

G

N

A

E

D

K

F

L

I

A

I

E

N

C

K

M

L

G

G

K

A

S

E

G

G

K

G

W

E

T

V

S

A

F

I

V

I

-

E

G

G

S

K

L

A

G

G

S

N

L

A

T

S

H

G

V

E

Q

A

W
x
R

A

R

D

N

G

G

G

A

A

T

E

E

N

A

A

F

K

K

Y

A

P

N

E

Q

M

I

E

K

L

L

V

V

S

A

E

S

K

Q

N

P

E

A

S

D

F
x
W

N

-

D

D

A

R

N

I

K

K

A

A

Y

L

E

D

K

V

A

A

R

T

E

N

I

V

L

L

R

Q

K

R

Y

N

P

P

D

N

I

L

K

K

G

A

F

F

Q

Y

G

C

G

A

S
x
N

A

D

I

T

D

M

V

A

I

M

G

G

I

V

G

A

R

Q

A

A

V

V

E

A

E

N

A

A

G

G

L

K

V

I

G

G

K

K

V

V

C

L

V

V

G

G

L

T

-
x
D

G

G

L

I

P

P

K

-

D

E

T

A

A

R

K

K

Y

M

L

V

E

E

S

A

G

G

A

Q

V

M

Q

T

S

A

I

T

S

V

F

A

W
x
Q

D

N

P

P

K

A

D

D

A

I

G

G

binding beta-D-ribopyranose: D91 (≠ E118), R151 (≠ W170), W175 (≠ F194), N201 (≠ S221), D227 (vs. gap), Q247 (≠ W267)

Sites not aligning to the query:

binding beta-D-ribopyranose: 13, 15

4wutA Crystal structure of an abc transporter solute binding protein (ipr025997) from agrobacterium vitis (avi_5133, target efi-511220) with bound d-fucose
25% identity, 70% coverage: 31:263/333 of query aligns to 3:238/290 of 4wutA

query
sites
4wutA

V

I

V

A

T

V

V

I

V

V

K
|
K

V

T

T

V

G

N

E

S

N

T

W

F

F
x
W

T

Q

R

N

M

V

E

Q

E

K

G

G

V

A

-

D

V

A

A

A

Y

I

G

G

K

K

D

Q

N

K

P

A

A

H

V

T

S

I

T

T

S

F

Q

Q

I

-

G

G

P

P

A

A

K

A

A
x
E

D

S

A

A

-

I

A

A

Q

D

Q

Q

L

V

R

N

L

M

I

V

E

E

D

N

L

A

V

V

A

N

K

R

N

K

V

V

N

D

A

A

I

I

A

L

V

L

V

A

P

P

M

S

D

D

P

P

S

D

A

A

L

L

E

V

G

P

V

A

F

V

K

K

R

K

A

A

M

W

N

E

R

A

G

R

I

I

K

P

I

V

T

I

H

I

E
x
D

A

S

D

M

S

L

L

S

K

K

N

D

T

A

Q

E

V

K

D

Y

I

Y

E

Q

A

A

F

F

-

L

-

A

-

T

D

D

N

N

K

K

V

A

F

A

G

G

A

E

R

L

F

A

N

A

E

K

K

A

L

M

A

I

E

Q

C

K

M

V

G

G

K

T

S

E

G

G

K

K

W

I

T

A

S

V

F

M

V

S

G

Y

S

V

L

A

G

G

S

A

L

G

T

S

H

E

V

I

Q

G

W
x
R

A

V

D

G

G

G

G

F

A

T

E

D

N

Y

A

I

K
|
K

K

A

Y

N

P
x
S

E

K

M

L

E

Q

L

I

V

V

S

G

E

P

K

Y

N

Y

E

S

S

Q

F

S

N

Q

D

M

A

A

N

T

K

-

A

A

Y

L

E
x
N

K

Q

A

T

R

T

E
x
D

I

V

L

L

R

A

K

A

Y

N

P

A

D

D

I

L

K

K

G

G

F

I

Q

F

G

G

G

A

S
x
N

A

E

I

P

D

T

V

A

I

I

G

G

I

M

G

G

R

R

A

A

V

I

E

K

E

Q

A

A

G

G

L

K

V

A

G

G

K

K

V

L

C

V

V

A

V

I

G

G

L

F

G
x
D

L

G

P

N

K

Q

D

D

T

L

A

Q

K

E

Y

F

L

V

E

K

S

D

G

G

A

T

V

L

Q

E

S

A

binding calcium ion: K155 (= K179), S158 (≠ P182), N177 (≠ E202), D181 (≠ E206)
binding beta-D-fucopyranose: K8 (= K36), W15 (≠ F43), E42 (≠ A70), D91 (≠ E118), R146 (≠ W170), N196 (≠ S221), D222 (≠ G247)

Sites not aligning to the query:

binding beta-D-fucopyranose: 242

4wzzA Crystal structure of an abc transporter solute binding protein (ipr025997) from clostridium phytofermentas (cphy_0583, target efi- 511148) with bound l-rhamnose
24% identity, 62% coverage: 67:273/333 of query aligns to 42:253/321 of 4wzzA

query
sites
4wzzA

P

P

A

K

K

S

A

A

D

T

A

A

A

E

Q

D

Q

Q

L

I

R

T

L

C

I

I

E

N

D

N

L

A

V

I

A

S

K

Q

N

G

V

V

N

D

A

C

I

I

A

A

V

I

V

A

P

A

M

N

D

D

P

T

S

D

A

A

L

L

E

E

G

P

V

A

F

L

K

T

R

E

A

A

M

K

N

N

R

Q

G

G

I

I

K

H

I

V

I

L

T

S

H

L

E
x
D

A

S

D

A

S

T

L

N

K

A

N

N

T

S

Q

R

V

-

D

-

I

-

E

K

A

V

F

F

D

V

N

N

K

Q

V

A

F

G

G

T

A

T

R

Q

F

I

N

A

E

Q

K

A

L

L

A

M

E

D

C

A

M

I

-

L

-

D

-

I

-

S

G

G

K

G

S

S

G

G

K

D

W

W

T

A

S

V

F

L

V

S

G

A

S

A

L

S

G

T

S

A

L

T

T
x
N

H

Q

V

N

Q

A

W

W

A

I

D

D

G

G

-

M

-

K

G

T

A

V

E

M

N

Q

A

D

K

S

K

K

Y

Y

P

S

E

K

M

L

E

N

L

L

V

I

S

G

E

V

K

Y

N

Y

E

G

S

D

F

-

N
x
D

D

E

A

Y

N

Q

K

A

A

S

Y

C

E

D

K

Q

A

T

R

E

E

A

I

I

L

L

R

A

K

A

Y

D

P

P

D

N

I

I

K

K

G

V

F

I

Q

C

G

A

G

P

S
x
T

A
x
T

I

V

D

G

V

I

I

M

G

A

I

A

G

A

R

K

A

V

V

L

E

Q

E

D

A

K

G

G

L

L

V

S

G

G

K

K

V

V

C

K

V

L

V

T

G

G

L

L

G

G

L

L

P

P

K

S

D

E

T

M

A

A

K

D

Y

Y

L

I

E

G

S

D

G

D

A

D

V

Q

Q

H

S

S

I

C

S

P

F

Y

-

M

-

F

-

L

W
|
W

D

N

P

P

K

I

D

Q

A

L

G

G

binding alpha-L-rhamnopyranose: D93 (≠ E118), N142 (≠ T166), D172 (≠ N195), T198 (≠ S221), T199 (≠ A222), W247 (= W267)

Sites not aligning to the query:

binding alpha-L-rhamnopyranose: 12, 16, 18

5dkvA Crystal structure of an abc transporter solute binding protein from agrobacterium vitis(avis_5339, target efi-511225) bound with alpha-d- tagatopyranose
27% identity, 70% coverage: 75:307/333 of query aligns to 52:293/303 of 5dkvA

query
sites
5dkvA

Q

Q

L

V

R

P

L

V

I

L

E

D

D

A

L

V

V

I

A

A

K

K

N

K

V

P

N

D

A

A

I

I

A

L

V

I

V

A

P

P

M

T

D

D

P

T

S

T

A

Q

L

L

E

V

G

Q

V

P

F

L

K

K

R

K

A

A

M

A

N

D

R

A

G

G

I

I

K

P

I

M

I

I

T

T

H

-

E

-

A

V

D
|
D

S

T

L

F

K

I

N

G

T

T

-

G

-

D

-

Y

-

Q

-

T

-

G

-

A

-

G

-

D

-

G

-

D

-

F

Q

P

V

L

D

S

I

Y

E

I

A

A

F

S

D

D

N

N

K

V

V

L

F

G

G

G

A

E

R

I

F

A

N

A

E

R

K

S

L

L

A

A

E

L

C

A

M

I

G

G

K

D

S

K

G

G

K

K

-

V

W

Y

T

V

S

S

F

N

V

V

G

K

S

P

L

-

G

G

S

V

L

S

T
|
T

H

T

V

D

Q

Q

W
x
R

A

E

D

Q

G

G

G

F

A

K

E

S

N

E

A

M

K

A

K

K

Y

H

P

P

E

G

M

I

E

T

L

V

V

L

S

-

E

E

K

T

N

Q

E

F

S

N

F

D

N

N

D

D

A

A

N

N

K

K

A

A

Y

A

E

S

K

Q

A

L

R

Q

E

A

I

V

L

Y

R

A

K

R

Y

N

P

P

D

D

I

L

K

A

G

G

F

V

Q

F

G

G

G

A

S
x
N

A
x
L

I

F

D

S

V

G

I

L

G

G

I

S

G

A

R

N

A

G

V

V

E

Q

A

A

G

G

L

Q

V

S

G

G

K

T

V

I

C

K

V

V

G

A

L

F

G
x
D

L

A

P

P

K

G

D

S

T

V

A

V

K

D

Y

N

L

L

E

K

S

S

G

G

A

L

V

I

Q

D

S

F

I

A

S

I

F

A

W
x
Q

D

H

P

P

K

A

D

E

A

I

G

G

Y

Y

-

Y

-

G

V

V

M

I

N

S

K

A

V

Y

A

A

Q

H

L

L

V

T

I

-

E

-

G

G

K

Q

E

S

I

I

T

P

D

T

G

K

M

I

D

G

L

T

G

G

V

F

P

T

G

V

Y

I

N

N

K

K

V

S

S

N

V

V

K

T

N

D

binding alpha-D-tagatopyranose: D95 (= D120), T153 (= T166), R157 (≠ W170), N207 (≠ S221), L208 (≠ A222), D233 (≠ G247), Q253 (≠ W267)

Sites not aligning to the query:

binding alpha-D-tagatopyranose: 18, 20

7e7mC Crystal structure analysis of the streptococcus agalactiae ribose binding protein rbsb
24% identity, 77% coverage: 35:289/333 of query aligns to 15:263/284 of 7e7mC

query
sites
7e7mC

V

I

K

S

V

T

T

T

G

N

E
x
N

N

P

W
x
Y

F
|
F

T

V

R

A

M

M

E

K

E

D

G

G

V

I

V

D

A

K

Y

Y

G

A

K

S

D

-

N

N

P

K

A

K

V

I

S

S

T

I

S

-

Q

K

I

V

G

A

P

D

A

A

K

Q

A

D

D

D

A

A

Q

R

Q

Q

L

A

R

D

L

D

I

V

E

Q

D

N

L

F

V

I

A

S

K

Q

N

N

V

V

N

D

A

A

I

I

A

L

V

I

V

N

P

P

M

V

D

D

P

S

S

K

A

A

L

I

E

V

G

T

V

A

F

I

K

K

R

S

A

A

M

N

N

N

R

A

G

N

I

I

K

P

I

V

I

I

T

L

H

M

E
x
D

A
x
R

D

G

S

S

L

E

K

G

N

G

T

K

Q

V

V

L

D

T

I

T

E

V

A

A

F

S

D

D

N

N

K

V

V

A

F

A

G

G

A

K

R

M

F

A

N

A

E

D

K

Y

L

A

A

V

E

K

C

K

M

L

G

G

K

K

S

K

G

A

K

K

W

A

T

F

S

E

F

L

V

S

G

G

S

V

L

P

G

G

S

A

L

S

T

A

H

T

V

V

Q

D

W
x
R

A

G

D

K

G

G

G

F

A

H

E

S

N

V

A

A

K

K

K

-

Y

-

P

S

E

K

M

L

E

D

L

M

V

L

S

S

E

S

K

Q

N

S

E

A

S

N

F
|
F

N

D

D

R

A

A

N

-

K

K

A

A

Y

L

E

N

K

T

A

T

R

Q

E

N

I

M

L

I

R

Q

K

G

Y

H

P

K

D

D

I

V

K

Q

G

I

F

I

Q

F

G

A

G

Q

S
x
N

A

D

I

E

D

M

V

A

I

L

G

G

I

A

G

A

R

Q

A

A

V

V

E

K

E

S

A

A

G

G

L

L

V

-

G

Q

K

N

V

V

C

L

V

I

V

V

G

G

L

I

G
x
D

L

G

P

Q

K

P

D

D

T

A

A

H

K

D

Y

A

L

I

E

K

S

K

G

G

A

D

V

I

Q

S

S

A

I

T

S

I

F

A

W
x
Q

D

Q

P

P

K

A

D

K

A

M

G

G

Y

E

V

I

M

A

N

I

K

Q

V

A

A

A

Q

I

L

D

V

Y

I

Y

E

K

G

G

K

K

E

K

I

V

binding beta-D-ribopyranose: N20 (≠ E40), Y22 (≠ W42), F23 (= F43), D96 (≠ E118), R97 (≠ A119), R148 (≠ W170), F170 (= F194), N196 (≠ S221), D221 (≠ G247), Q241 (≠ W267)

2ioyA Crystal structure of thermoanaerobacter tengcongensis ribose binding protein (see paper)
24% identity, 66% coverage: 71:289/333 of query aligns to 41:257/274 of 2ioyA

query
sites
2ioyA

D

D

A

S

Q

K

Q

E

L

L

R

S

L

N

I

V

E

E

D

D

L

L

V

I

A

Q

K

Q

N

K

V

V

N

D

A

V

I

L

A

L

V

I

V

N

P

P

M

V

D

D

P

S

S

D

A

A

L

V

E

V

G

T

V

A

F

I

K

K

R

E

A

A

M

N

N

S

R

K

G

N

I

I

K

P

I

V

I

I

T

T

H

I

E
x
D

A
x
R

D

S

S

A

L

N

K

G

N

G

T

D

Q

V

V

V

D

C

I

H

E

I

A

A

F

S

D

D

N

N

K

V

V

K

F

G

G

G

A

E

R

M

F

A

N

A

E

E

K

F

L

I

A

A

E

K

C

A

M

L

G

K

K

G

S

K

G

G

K

N

W

V

T

V

S

E

F

L

V

E

G

G

S

I

L

P

G

G

S

A

L

S

T
x
A

H

A

V

R

Q

D

W
x
R

A

G

D

K

G

G

G

F

A

D

E

E

N

A

A

I

K

A

K

K

Y

Y

P

P

E

D

M

I

E

K

L

I

V

V

S

A

E

K

K

Q

N

A

E

A

S

D

F
|
F

N

-

D

D

A

R

N

S

K

K

A

G

Y

L

E

S

K

V

A

M

R

E

E

N

I

I

L

L

R

Q

K

A

Y

Q

P

P

D

K

I

I

K

D

G

A

F

V

Q

F

G

A

G

Q

S
x
N

A

D

I

E

D

M

V

A

I

L

G

G

I

A

G

I

R

K

A

A

V

I

E

E

E

A

A

A

G

N

L

R

V

Q

G

G

K

-

V

I

C

I

V

V

G

G

L

F

G
x
D

L

G

P

T

K

E

D

D

T

A

A

L

K

K

Y

A

L

I

E

K

S

E

G

G

A

K

V

M

Q

A

S

A

I

T

S

I

F

A

W
x
Q

D

Q

P

P

K

A

D

L

A

M

G

G

Y

S

V

L

M

G

N

V

K

E

V

M

A

A

Q

D

L

K

V

Y

I

L

E

K

G

G

K

E

E

K

I

I

binding beta-D-ribopyranose: D88 (≠ E118), R89 (≠ A119), A136 (≠ T166), R140 (≠ W170), F164 (= F194), N190 (≠ S221), D215 (≠ G247), Q235 (≠ W267)

Sites not aligning to the query:

binding beta-D-ribopyranose: 12, 14, 15

5xssA Xylfii molecule (see paper)
21% identity, 78% coverage: 29:287/333 of query aligns to 4:260/274 of 5xssA

query
sites
5xssA

P

P

S

K

V

I

V

V

T

L

V

I

V

S

K
x
H

V

I

T

K

G

T

E

N

N

P

W

Y

F
x
W

T

L

R

D

M

I

E

K

E

A

G

G

V

A

V

E

A

R

Y

A

G

A

K

K

D

E

N

R

P

G

A

A

V

V

S

-

T

V

S

E

Q

F

I

L

G

G

P

P

A

T

K

T

A

A

D

S

A

T

A

E

Q

D

Q

G

L

L

R

K

L

L

I

F

E

D

D

M

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|
S

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|
A

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binding beta-D-xylopyranose: H11 (≠ K36), W18 (≠ F43), D92 (≠ E118), R144 (≠ W170), S194 (= S221), A195 (= A222), D220 (≠ G247), Q240 (≠ W267)

Query Sequence

>SM_b20316 FitnessBrowser__Smeli:SM_b20316
MSVYKKIAACTVALGALCAAQLAVAQDAPSVVTVVKVTGENWFTRMEEGVVAYGKDNPAV
STSQIGPAKADAAQQLRLIEDLVAKNVNAIAVVPMDPSALEGVFKRAMNRGIKIITHEAD
SLKNTQVDIEAFDNKVFGARFNEKLAECMGKSGKWTSFVGSLGSLTHVQWADGGAENAKK
YPEMELVSEKNESFNDANKAYEKAREILRKYPDIKGFQGGSAIDVIGIGRAVEEAGLVGK
VCVVGLGLPKDTAKYLESGAVQSISFWDPKDAGYVMNKVAQLVIEGKEITDGMDLGVPGY
NKVSVKNGPGDGIIVVGEAWVDVDKSNYSQYPF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory